Pten cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pten
Entrez Gene ID	19211
Full Name	phosphatase and tensin homolog
Synonyms	2310035O07Rik,A130070J02Rik,AI463227,B430203M17Rik,MMAC1,PTENbeta,TEP1
General protein information	Preferred Names phosphatase and tensin homolog Names phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN mitochondrial PTENalpha mutated in multiple advanced cancers 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C1\|19 28.14 cM
Summary	This gene encodes a phosphatase with dual activity against phospholipids and proteins, and acts as a tumor-suppressor. The protein contains four structural domains, a PIP2-binding domain, a catalytic tensin-type phosphatase domain, a C2 tensin-type domain and a PDZ-binding domain. The protein belongs to the protein tyrosine phosphatase family. Deletion of this gene in mice contribute to tumorigenesis in multiple tissues. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008960.2	NP_032986.1	phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
XM_006526769.2	XP_006526832.1	phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pten	NP_032986.1
Gallus gallus (chicken)	PTEN	XP_421555.2
Drosophila melanogaster (fruit fly)	Pten	NP_001162933.1
	PTEN	XP_001102542.1
Bos taurus (cattle)	PTEN	XP_002698416.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009628	XP_553421.2
Pan troglodytes (chimpanzee)	PTEN	XP_521544.3
Danio rerio (zebrafish)	ptenb	NP_001001822.1
Homo sapiens (human)	PTEN	NP_000305.3
Rattus norvegicus (Norway rat)	Pten	NP_113794.1
Canis lupus familiaris (dog)	PTEN	NP_001003192.1
Xenopus tropicalis (tropical clawed frog)	pten	NP_001116943.1

Gene Ontology
Bibliography

Related articles in PubMed

High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice.
Suzuki A, de la Pompa JL, Stambolic V, Elia AJ, Sasaki T, del Barco Barrantes I, Ho A, Wakeham A, Itie A, Khoo W, Fukumoto M, Mak TW
Current biology : CB8(21)1169-78(1998 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CFTR attaches tumor suppressor PTEN to the membrane and promotes anti Pseudomonas aeruginosa immunity.
Title: Cystic Fibrosis Transmembrane Conductance Regulator Attaches Tumor Suppressor PTEN to the Membrane and Promotes Anti Pseudomonas aeruginosa Immunity.

Homozygous deletion of the Pten gene in neuronal subpopulations in the mouse serves as a valuable model of epilepsy caused by mTOR hyperactivation. To better understand how gene deletions lead to altered neuronal activity, we investigated the synaptic and network effects that occur 1 week after Pten deletion.
Title: PTEN Loss Increases the Connectivity of Fast Synaptic Motifs and Functional Connectivity in a Developing Hippocampal Network.

these results demonstrate that miR-26b might participate in regulating adipogenic differentiation in 3T3-L1 cells by inhibiting the PTEN expression, further highlighting the importance of miRNA in adipogenesis.
Title: miR-26b Promotes 3T3-L1 Adipocyte Differentiation Through Targeting PTEN.

further molecular mechanisms of HOTAIR action in cardiac hypertrophy (CH) demonstrated that HOTAIR may act as a competing endogenous RNA (ceRNA) for miR-19, thereby modulating the dis-inhibition of its endogenous target PTEN and playing an important role in inhibiting CH progress.
Title: HOTAIR functions as a competing endogenous RNA to regulate PTEN expression by inhibiting miR-19 in cardiac hypertrophy.

PTEN counteracts FBXL2 to promote IP3R3- and Ca(2+)-mediated apoptosis limiting tumour growth
Title: PTEN counteracts FBXL2 to promote IP3R3- and Ca

The following Pten gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pten cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu55311	XM_006526769.2 Latest version!	Mus musculus phosphatase and tensin homolog (Pten), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu23033	NM_008960.2 Latest version!	Mus musculus phosphatase and tensin homolog (Pten), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pten ( XM_006526769.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526832.1 Mus musculus phosphatase and tensin homolog (Pten), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55311
Clone ID Related Accession (Same CDS sequence)	XM_006526769.2
Accession Version	XM_006526769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTGTG CATATTTATT GCATCGGGGC AAATTTTTAA AGGCACAAGA GGCCCTAGAT 
TTTTATGGGG AAGTAAGGAC CAGAGACAAA AAGGGAGTCA CAATTCCCAG TCAGAGGCGC 
TATGTATATT ATTATAGCTA CCTGCTAAAA AATCACCTGG ATTACAGACC CGTGGCACTG 
CTGTTTCACA AGATGATGTT TGAAACTATT CCAATGTTCA GTGGCGGAAC TTGCAATCCT 
CAGTTTGTGG TCTGCCAGCT AAAGGTGAAG ATATATTCCT CCAATTCAGG ACCCACGCGG 
CGGGAGGACA AGTTCATGTA CTTTGAGTTC CCTCAGCCAT TGCCTGTGTG TGGTGATATC 
AAAGTAGAGT TCTTCCACAA ACAGAACAAG ATGCTCAAAA AGGACAAAAT GTTTCACTTT 
TGGGTAAATA CGTTCTTCAT ACCAGGACCA GAGGAAACCT CAGAAAAAGT GGAAAATGGA 
AGTCTTTGTG ATCAGGAAAT CGATAGCATT TGCAGTATAG AGCGTGCAGA TAATGACAAG 
GAGTATCTTG TACTCACCCT AACAAAAAAC GATCTTGACA AAGCAAACAA AGACAAGGCC 
AACCGATACT TCTCTCCAAA TTTTAAGGTG AAACTATACT TTACAAAAAC AGTAGAGGAG 
CCATCAAATC CAGAGGCTAG CAGTTCAACT TCTGTGACTC CAGATGTTAG TGACAATGAA 
CCTGATCATT ATAGATATTC TGACACCACT GACTCTGATC CAGAGAATGA ACCTTTTGAT 
GAAGATCAGC ATTCACAAAT TACAAAAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526832.1
CDS	1308..2120
Translation	MICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV

Target ORF information:

RefSeq Version	XM_006526769.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatase and tensin homolog (Pten), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526769.2

ORF Insert Sequence:

ATGATTTGTG CATATTTATT GCATCGGGGC AAATTTTTAA AGGCACAAGA GGCCCTAGAT 
TTTTATGGGG AAGTAAGGAC CAGAGACAAA AAGGGAGTCA CAATTCCCAG TCAGAGGCGC 
TATGTATATT ATTATAGCTA CCTGCTAAAA AATCACCTGG ATTACAGACC CGTGGCACTG 
CTGTTTCACA AGATGATGTT TGAAACTATT CCAATGTTCA GTGGCGGAAC TTGCAATCCT 
CAGTTTGTGG TCTGCCAGCT AAAGGTGAAG ATATATTCCT CCAATTCAGG ACCCACGCGG 
CGGGAGGACA AGTTCATGTA CTTTGAGTTC CCTCAGCCAT TGCCTGTGTG TGGTGATATC 
AAAGTAGAGT TCTTCCACAA ACAGAACAAG ATGCTCAAAA AGGACAAAAT GTTTCACTTT 
TGGGTAAATA CGTTCTTCAT ACCAGGACCA GAGGAAACCT CAGAAAAAGT GGAAAATGGA 
AGTCTTTGTG ATCAGGAAAT CGATAGCATT TGCAGTATAG AGCGTGCAGA TAATGACAAG 
GAGTATCTTG TACTCACCCT AACAAAAAAC GATCTTGACA AAGCAAACAA AGACAAGGCC 
AACCGATACT TCTCTCCAAA TTTTAAGGTG AAACTATACT TTACAAAAAC AGTAGAGGAG 
CCATCAAATC CAGAGGCTAG CAGTTCAACT TCTGTGACTC CAGATGTTAG TGACAATGAA 
CCTGATCATT ATAGATATTC TGACACCACT GACTCTGATC CAGAGAATGA ACCTTTTGAT 
GAAGATCAGC ATTCACAAAT TACAAAAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pten ( NM_008960.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032986.1 Mus musculus phosphatase and tensin homolog (Pten), mRNA.

Price & Availability↑

CloneID	OMu23033
Clone ID Related Accession (Same CDS sequence)	NM_008960.2
Accession Version	NM_008960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92437.1, AK138025.1, CK616227.1 and BU701098.1. On Feb 2, 2006 this sequence version replaced NM_008960.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK076980.1, AK088717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AATATTATTG CTATGGGATT TCCTGCAGAA 
AGACTTGAAG GTGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG 
CATAAAAACC ATTACAAGAT ATACAATCTA TGTGCTGAGA GACATTATGA CACCGCCAAA 
TTTAACTGCA GAGTTGCACA GTATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT 
ATCAAACCCT TCTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA 
GCAATTCACT GTAAAGCTGG AAAGGGACGG ACTGGTGTAA TGATTTGTGC ATATTTATTG 
CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TTTATGGGGA AGTAAGGACC 
AGAGACAAAA AGGGAGTCAC AATTCCCAGT CAGAGGCGCT ATGTATATTA TTATAGCTAC 
CTGCTAAAAA ATCACCTGGA TTACAGACCC GTGGCACTGC TGTTTCACAA GATGATGTTT 
GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA 
AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACGCGGC GGGAGGACAA GTTCATGTAC 
TTTGAGTTCC CTCAGCCATT GCCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA 
CAGAACAAGA TGCTCAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC GTTCTTCATA 
CCAGGACCAG AGGAAACCTC AGAAAAAGTG GAAAATGGAA GTCTTTGTGA TCAGGAAATC 
GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AGTATCTTGT ACTCACCCTA 
ACAAAAAACG ATCTTGACAA AGCAAACAAA GACAAGGCCA ACCGATACTT CTCTCCAAAT 
TTTAAGGTGA AACTATACTT TACAAAAACA GTAGAGGAGC CATCAAATCC AGAGGCTAGC 
AGTTCAACTT CTGTGACTCC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT 
GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TTCACAAATT 
ACAAAAGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032986.1
CDS	869..2080
Translation	MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV

Target ORF information:

RefSeq Version	NM_008960.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatase and tensin homolog (Pten), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008960.2

ORF Insert Sequence:

ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AATATTATTG CTATGGGATT TCCTGCAGAA 
AGACTTGAAG GTGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG 
CATAAAAACC ATTACAAGAT ATACAATCTA TGTGCTGAGA GACATTATGA CACCGCCAAA 
TTTAACTGCA GAGTTGCACA GTATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT 
ATCAAACCCT TCTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA 
GCAATTCACT GTAAAGCTGG AAAGGGACGG ACTGGTGTAA TGATTTGTGC ATATTTATTG 
CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TTTATGGGGA AGTAAGGACC 
AGAGACAAAA AGGGAGTCAC AATTCCCAGT CAGAGGCGCT ATGTATATTA TTATAGCTAC 
CTGCTAAAAA ATCACCTGGA TTACAGACCC GTGGCACTGC TGTTTCACAA GATGATGTTT 
GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA 
AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACGCGGC GGGAGGACAA GTTCATGTAC 
TTTGAGTTCC CTCAGCCATT GCCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA 
CAGAACAAGA TGCTCAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC GTTCTTCATA 
CCAGGACCAG AGGAAACCTC AGAAAAAGTG GAAAATGGAA GTCTTTGTGA TCAGGAAATC 
GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AGTATCTTGT ACTCACCCTA 
ACAAAAAACG ATCTTGACAA AGCAAACAAA GACAAGGCCA ACCGATACTT CTCTCCAAAT 
TTTAAGGTGA AACTATACTT TACAAAAACA GTAGAGGAGC CATCAAATCC AGAGGCTAGC 
AGTTCAACTT CTGTGACTCC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT 
GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TTCACAAATT 
ACAAAAGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice.
Current biology : CB8(21)1169-78(1998 Oct)
Suzuki A,de la Pompa JL,Stambolic V,Elia AJ,Sasaki T,del Barco Barrantes I,Ho A,Wakeham A,Itie A,Khoo W,Fukumoto M,Mak TW

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Pten cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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