Ptch1 cDNA ORF clone, Mus musculus(house mouse)

The following Ptch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu68507 XM_011244497.2
Latest version!
Mus musculus patched 1 (Ptch1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu48694 XM_006517162.3
Latest version!
Mus musculus patched 1 (Ptch1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu48694 XM_006517163.3
Latest version!
Mus musculus patched 1 (Ptch1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu48692 NM_001328514.1
Latest version!
Mus musculus patched 1 (Ptch1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu48692 XM_006517160.3
Latest version!
Mus musculus patched 1 (Ptch1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu22749 NM_008957.3
Latest version!
Mus musculus patched 1 (Ptch1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OMu22749 XM_006517159.3
Latest version!
Mus musculus patched 1 (Ptch1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu68507
    Clone ID Related Accession (Same CDS sequence) XM_011244497.2
    Accession Version XM_011244497.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein patched homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG
    CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC
    AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA
    GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT
    GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC
    TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA
    CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA
    GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC
    AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC
    TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG
    AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC
    CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT
    CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG
    GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG
    GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA
    GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC
    TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG
    TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC
    TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG
    ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA
    GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT
    CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT
    TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT
    GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA
    GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG
    TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC
    TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT
    GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG
    TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC
    CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC
    CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT
    CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA
    CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT
    CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA
    AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC
    TCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242799.1
    CDS144..2732
    Translation

    Target ORF information:

    RefSeq Version XM_011244497.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus patched 1 (Ptch1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244497.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA 
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG
    CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC
    AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA
    GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT
    GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC
    TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA
    CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA
    GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC
    AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC
    TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG
    AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC
    CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT
    CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG
    GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG
    GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA
    GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC
    TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG
    TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC
    TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG
    ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA
    GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT
    CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT
    TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT
    GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA
    GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG
    TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC
    TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT
    GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG
    TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC
    CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC
    CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT
    CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA
    CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT
    CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA
    AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC
    TCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48694
    Clone ID Related Accession (Same CDS sequence) XM_006517163.3 , XM_006517162.3
    Accession Version XM_006517163.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein patched homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517163.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
    ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC
    ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG
    GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG
    CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC
    ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA
    AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC
    AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT
    GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG
    GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC
    GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA
    AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT
    GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT
    GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT
    CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC
    TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA
    GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA
    GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC
    CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC
    ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC
    GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG
    CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA
    CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT
    GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA
    GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT
    GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG
    CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC
    AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC
    AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT
    GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT
    GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC
    AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC
    GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA
    GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT
    GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC
    ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC
    ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG
    AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG
    TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC
    TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG
    CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC
    CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT
    GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG
    TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC
    CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT
    CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC
    TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG
    CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC
    CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG
    GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA
    GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517226.1
    CDS429..4322
    Translation

    Target ORF information:

    RefSeq Version XM_006517163.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus patched 1 (Ptch1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517163.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
    ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC
    ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG
    GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG
    CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC
    ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA
    AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC
    AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT
    GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG
    GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC
    GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA
    AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT
    GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT
    GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT
    CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC
    TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA
    GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA
    GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC
    CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC
    ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC
    GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG
    CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA
    CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT
    GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA
    GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT
    GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG
    CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC
    AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC
    AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT
    GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT
    GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC
    AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC
    GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA
    GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT
    GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC
    ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC
    ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG
    AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG
    TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC
    TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG
    CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC
    CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT
    GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG
    TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC
    CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT
    CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC
    TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG
    CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC
    CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG
    GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA
    GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48694
    Clone ID Related Accession (Same CDS sequence) XM_006517163.3 , XM_006517162.3
    Accession Version XM_006517162.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein patched homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517162.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
    ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC
    ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG
    GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG
    CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC
    ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA
    AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC
    AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT
    GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG
    GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC
    GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA
    AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT
    GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT
    GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT
    CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC
    TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA
    GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA
    GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC
    CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC
    ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC
    GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG
    CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA
    CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT
    GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA
    GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT
    GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG
    CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC
    AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC
    AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT
    GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT
    GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC
    AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC
    GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA
    GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT
    GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC
    ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC
    ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG
    AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG
    TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC
    TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG
    CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC
    CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT
    GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG
    TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC
    CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT
    CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC
    TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG
    CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC
    CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG
    GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA
    GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517225.1
    CDS163..4056
    Translation

    Target ORF information:

    RefSeq Version XM_006517162.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus patched 1 (Ptch1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517162.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACT CCAAAAGAAG AAGGCGCTAA TGTTCTGACC 
    ACAGAGGCTC TCCTGCAACA CCTGGACTCA GCACTCCAGG CCAGTCGTGT GCACGTCTAC
    ATGTATAACA GGCAATGGAA GTTGGAACAT TTGTGCTACA AATCAGGGGA ACTTATCACG
    GAGACAGGTT ACATGGATCA GATAATAGAA TACCTTTACC CTTGCTTAAT CATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCAAAGCTA CAGTCCGGGA CAGCATACCT CCTAGGTAAG
    CCTCCTTTAC GGTGGACAAA CTTTGACCCC TTGGAATTCC TAGAAGAGTT AAAGAAAATA
    AACTACCAAG TGGACAGCTG GGAGGAAATG CTGAATAAAG CCGAAGTTGG CCATGGGTAC
    ATGGACCGGC CTTGCCTCAA CCCAGCCGAC CCAGATTGCC CTGCCACAGC CCCTAACAAA
    AATTCAACCA AACCTCTTGA TGTGGCCCTT GTTTTGAATG GTGGATGTCA AGGTTTATCC
    AGGAAGTATA TGCATTGGCA GGAGGAGTTG ATTGTGGGTG GTACCGTCAA GAATGCCACT
    GGAAAACTTG TCAGCGCTCA CGCCCTGCAA ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTATGAAC ACTTCAGGGG CTACGACTAT GTCTCTCACA TCAACTGGAA TGAAGACAGG
    GCAGCCGCCA TCCTGGAGGC CTGGCAGAGG ACTTACGTGG AGGTGGTTCA TCAAAGTGTC
    GCCCCAAACT CCACTCAAAA GGTGCTTCCC TTCACAACCA CGACCCTGGA CGACATCCTA
    AAATCCTTCT CTGATGTCAG TGTCATCCGA GTGGCCAGCG GCTACCTACT GATGCTTGCC
    TATGCCTGTT TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGTTGGTTGC GCTGTCAGTG GCTGCAGGAT TGGGCCTCTG CTCCTTGATT
    GGCATTTCTT TTAATGCTGC GACAACTCAG GTTTTGCCGT TTCTTGCTCT TGGTGTTGGT
    GTGGATGATG TCTTCCTCCT GGCCCATGCA TTCAGTGAAA CAGGACAGAA TAAGAGGATT
    CCATTTGAGG ACAGGACTGG GGAGTGCCTC AAGCGCACCG GAGCCAGCGT GGCCCTCACC
    TCCATCAGCA ATGTCACCGC CTTCTTCATG GCCGCATTGA TCCCTATCCC TGCCCTGCGA
    GCGTTCTCCC TCCAGGCTGC TGTGGTGGTG GTATTCAATT TTGCTATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAC AGACGTGAGG ACAGAAGATT GGATATTTTC
    TGCTGTTTCA CAAGCCCCTG TGTCAGCAGG GTGATTCAAG TTGAGCCACA GGCCTACACA
    GAGCCTCACA GTAACACCCG GTACAGCCCC CCACCCCCAT ACACCAGCCA CAGCTTCGCC
    CACGAAACCC ATATCACTAT GCAGTCCACC GTTCAGCTCC GCACAGAGTA TGACCCTCAC
    ACGCACGTGT ACTACACCAC CGCCGAGCCA CGCTCTGAGA TCTCTGTACA GCCTGTTACC
    GTCACCCAGG ACAACCTCAG CTGTCAGAGT CCCGAGAGCA CCAGCTCTAC CAGGGACCTG
    CTCTCCCAGT TCTCAGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGCAC CAAGTGGACA
    CTCTCTTCGT TTGCAGAGAA GCACTATGCT CCTTTCCTCC TGAAACCCAA AGCCAAGGTT
    GTGGTAATCC TTCTTTTCCT GGGCTTGCTG GGGGTCAGCC TTTATGGGAC CACCCGAGTG
    AGAGACGGGC TGGACCTCAC GGACATTGTT CCCCGGGAAA CCAGAGAATA TGACTTCATA
    GCTGCCCAGT TCAAGTACTT CTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACCT ACTTTACGAC CTTCATAAGA GTTTCAGCAA TGTGAAGTAT
    GTCATGCTGG AGGAGAACAA GCAACTTCCC CAAATGTGGC TGCACTACTT TAGAGACTGG
    CTTCAAGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CTGGGAGGAT CATGCCAAAC
    AATTATAAAA ATGGATCAGA TGACGGGGTC CTCGCTTACA AACTCCTGGT GCAGACTGGC
    AGCCGAGACA AGCCCATCGA CATTAGTCAG TTGACTAAAC AGCGTCTGGT AGACGCAGAT
    GGCATCATTA ATCCGAGCGC TTTCTACATC TACCTGACCG CTTGGGTCAG CAACGACCCT
    GTAGCTTACG CTGCCTCCCA GGCCAACATC CGGCCTCACC GGCCGGAGTG GGTCCATGAC
    AAAGCCGACT ACATGCCAGA GACCAGGCTG AGAATCCCAG CAGCAGAGCC CATCGAGTAC
    GCTCAGTTCC CTTTCTACCT CAACGGCCTA CGAGACACCT CAGACTTTGT GGAAGCCATA
    GAAAAAGTGA GAGTCATCTG TAACAACTAT ACGAGCCTGG GACTGTCCAG CTACCCCAAT
    GGCTACCCCT TCCTGTTCTG GGAGCAATAC ATCAGCCTGC GCCACTGGCT GCTGCTATCC
    ATCAGCGTGG TGCTGGCCTG CACGTTTCTA GTGTGCGCAG TCTTCCTCCT GAACCCCTGG
    ACGGCCGGGA TCATTGTCAT GGTCCTGGCT CTGATGACCG TTGAGCTCTT TGGCATGATG
    GGCCTCATTG GGATCAAGCT GAGTGCTGTG CCTGTGGTCA TCCTGATTGC ATCTGTTGGC
    ATCGGAGTGG AGTTCACCGT CCACGTGGCT TTGGCCTTTC TGACAGCCAT TGGGGACAAG
    AACCACAGGG CTATGCTCGC TCTGGAGCAC ATGTTTGCTC CCGTTCTGGA CGGTGCTGTG
    TCCACTCTGC TGGGTGTACT GATGCTTGCA GGGTCCGAAT TTGATTTCAT TGTCAGATAC
    TTCTTTGCCG TCCTGGCCAT TCTCACCGTC TTGGGGGTTC TCAATGGACT GGTTCTGCTG
    CCTGTCCTCT TATCCTTCTT TGGACCGTGT CCTGAGGTGT CTCCAGCCAA TGGCCTAAAC
    CGACTGCCCA CTCCTTCGCC TGAGCCGCCT CCAAGTGTCG TCCGGTTTGC CGTGCCTCCT
    GGTCACACGA ACAATGGGTC TGATTCCTCC GACTCGGAGT ACAGCTCTCA GACCACGGTG
    TCTGGCATCA GTGAGGAGCT CAGGCAATAC GAAGCACAGC AGGGTGCCGG AGGCCCTGCC
    CACCAAGTGA TTGTGGAAGC CACAGAAAAC CCTGTCTTTG CCCGGTCCAC TGTGGTCCAT
    CCGGACTCCA GACATCAGCC TCCCTTGACC CCTCGGCAAC AGCCCCACCT GGACTCTGGC
    TCCTTGTCCC CTGGACGGCA AGGCCAGCAG CCTCGAAGGG ATCCCCCTAG AGAAGGCTTG
    CGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGACCG CTCAGGGCCC CGTGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAACGTCCA CCGCCATGGG CAGCTCTGTG CCCAGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CTTCGGTGAC TGTTGCTGTG CATCCCCCGC CTGGACCTGG GCGCAACCCC
    CGAGGGGGGC CCTGTCCAGG CTATGAGAGC TACCCTGAGA CTGATCACGG GGTATTTGAG
    GATCCTCATG TGCCTTTTCA TGTCAGGTGT GAGAGGAGGG ACTCAAAGGT GGAGGTCATA
    GAGCTACAGG ACGTGGAATG TGAGGAGAGG CCGTGGGGGA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48692
    Clone ID Related Accession (Same CDS sequence) XM_006517160.3 , NM_001328514.1
    Accession Version NM_001328514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product protein patched homolog 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147626.1, AB164615.1, CN535675.1 and BU700582.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC
    CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG
    ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC
    GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT
    CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA
    GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC
    TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA
    TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA
    GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA
    GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC
    ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA
    TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC
    GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC
    TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG
    GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC
    CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC
    CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC
    CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT
    GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA
    CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC
    AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT
    ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG
    CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC
    AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA
    GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT
    GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC
    TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA
    CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA
    GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC
    AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC
    TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG
    AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC
    CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT
    CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG
    GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG
    GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA
    GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC
    TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG
    TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC
    TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG
    ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA
    GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT
    CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT
    TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT
    GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA
    GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG
    TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC
    TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT
    GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG
    TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC
    CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC
    CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT
    CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA
    CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT
    CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA
    AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC
    TCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001315443.1
    CDS367..4515
    Translation

    Target ORF information:

    RefSeq Version NM_001328514.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus patched 1 (Ptch1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001328514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC
    CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG
    ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC
    GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT
    CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA
    GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC
    TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA
    TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA
    GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA
    GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC
    ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA
    TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC
    GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC
    TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG
    GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC
    CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC
    CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC
    CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT
    GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA
    CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC
    AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT
    ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG
    CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC
    AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA
    GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT
    GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC
    TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA
    CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA
    GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC
    AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC
    TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG
    AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC
    CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT
    CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG
    GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG
    GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA
    GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC
    TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG
    TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC
    TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG
    ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA
    GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT
    CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT
    TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT
    GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA
    GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG
    TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC
    TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT
    GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG
    TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC
    CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC
    CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT
    CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA
    CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT
    CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA
    AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC
    TCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48692
    Clone ID Related Accession (Same CDS sequence) XM_006517160.3 , NM_001328514.1
    Accession Version XM_006517160.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein patched homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517160.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC
    CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG
    ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC
    GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT
    CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA
    GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC
    TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA
    TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA
    GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA
    GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC
    ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA
    TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC
    GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC
    TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG
    GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC
    CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC
    CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC
    CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT
    GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA
    CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC
    AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT
    ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG
    CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC
    AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA
    GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT
    GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC
    TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA
    CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA
    GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC
    AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC
    TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG
    AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC
    CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT
    CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG
    GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG
    GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA
    GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC
    TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG
    TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC
    TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG
    ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA
    GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT
    CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT
    TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT
    GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA
    GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG
    TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC
    TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT
    GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG
    TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC
    CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC
    CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT
    CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA
    CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT
    CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA
    AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC
    TCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517223.1
    CDS523..4671
    Translation

    Target ORF information:

    RefSeq Version XM_006517160.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus patched 1 (Ptch1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517160.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAG AACTGCGGCA AGTTTTTGGT TGTGGGTCTC
    CTCATATTTG GGGCCTTCGC TGTGGGATTA AAGGCAGCTA ATCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG TGGACGAGTG AGTCGAGAAT TAAATTATAC CCGTCAGAAG
    ATAGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACTCCAAA AGAAGAAGGC
    GCTAATGTTC TGACCACAGA GGCTCTCCTG CAACACCTGG ACTCAGCACT CCAGGCCAGT
    CGTGTGCACG TCTACATGTA TAACAGGCAA TGGAAGTTGG AACATTTGTG CTACAAATCA
    GGGGAACTTA TCACGGAGAC AGGTTACATG GATCAGATAA TAGAATACCT TTACCCTTGC
    TTAATCATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCAA AGCTACAGTC CGGGACAGCA
    TACCTCCTAG GTAAGCCTCC TTTACGGTGG ACAAACTTTG ACCCCTTGGA ATTCCTAGAA
    GAGTTAAAGA AAATAAACTA CCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAAGCCGAA
    GTTGGCCATG GGTACATGGA CCGGCCTTGC CTCAACCCAG CCGACCCAGA TTGCCCTGCC
    ACAGCCCCTA ACAAAAATTC AACCAAACCT CTTGATGTGG CCCTTGTTTT GAATGGTGGA
    TGTCAAGGTT TATCCAGGAA GTATATGCAT TGGCAGGAGG AGTTGATTGT GGGTGGTACC
    GTCAAGAATG CCACTGGAAA ACTTGTCAGC GCTCACGCCC TGCAAACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA TGAACACTTC AGGGGCTACG ACTATGTCTC TCACATCAAC
    TGGAATGAAG ACAGGGCAGC CGCCATCCTG GAGGCCTGGC AGAGGACTTA CGTGGAGGTG
    GTTCATCAAA GTGTCGCCCC AAACTCCACT CAAAAGGTGC TTCCCTTCAC AACCACGACC
    CTGGACGACA TCCTAAAATC CTTCTCTGAT GTCAGTGTCA TCCGAGTGGC CAGCGGCTAC
    CTACTGATGC TTGCCTATGC CTGTTTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGTTG GTTGCGCTGT CAGTGGCTGC AGGATTGGGC
    CTCTGCTCCT TGATTGGCAT TTCTTTTAAT GCTGCGACAA CTCAGGTTTT GCCGTTTCTT
    GCTCTTGGTG TTGGTGTGGA TGATGTCTTC CTCCTGGCCC ATGCATTCAG TGAAACAGGA
    CAGAATAAGA GGATTCCATT TGAGGACAGG ACTGGGGAGT GCCTCAAGCG CACCGGAGCC
    AGCGTGGCCC TCACCTCCAT CAGCAATGTC ACCGCCTTCT TCATGGCCGC ATTGATCCCT
    ATCCCTGCCC TGCGAGCGTT CTCCCTCCAG GCTGCTGTGG TGGTGGTATT CAATTTTGCT
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATACAGACG TGAGGACAGA
    AGATTGGATA TTTTCTGCTG TTTCACAAGC CCCTGTGTCA GCAGGGTGAT TCAAGTTGAG
    CCACAGGCCT ACACAGAGCC TCACAGTAAC ACCCGGTACA GCCCCCCACC CCCATACACC
    AGCCACAGCT TCGCCCACGA AACCCATATC ACTATGCAGT CCACCGTTCA GCTCCGCACA
    GAGTATGACC CTCACACGCA CGTGTACTAC ACCACCGCCG AGCCACGCTC TGAGATCTCT
    GTACAGCCTG TTACCGTCAC CCAGGACAAC CTCAGCTGTC AGAGTCCCGA GAGCACCAGC
    TCTACCAGGG ACCTGCTCTC CCAGTTCTCA GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGCACCAAGT GGACACTCTC TTCGTTTGCA GAGAAGCACT ATGCTCCTTT CCTCCTGAAA
    CCCAAAGCCA AGGTTGTGGT AATCCTTCTT TTCCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGGACCACCC GAGTGAGAGA CGGGCTGGAC CTCACGGACA TTGTTCCCCG GGAAACCAGA
    GAATATGACT TCATAGCTGC CCAGTTCAAG TACTTCTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACCTACTTT ACGACCTTCA TAAGAGTTTC
    AGCAATGTGA AGTATGTCAT GCTGGAGGAG AACAAGCAAC TTCCCCAAAT GTGGCTGCAC
    TACTTTAGAG ACTGGCTTCA AGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACTGGG
    AGGATCATGC CAAACAATTA TAAAAATGGA TCAGATGACG GGGTCCTCGC TTACAAACTC
    CTGGTGCAGA CTGGCAGCCG AGACAAGCCC ATCGACATTA GTCAGTTGAC TAAACAGCGT
    CTGGTAGACG CAGATGGCAT CATTAATCCG AGCGCTTTCT ACATCTACCT GACCGCTTGG
    GTCAGCAACG ACCCTGTAGC TTACGCTGCC TCCCAGGCCA ACATCCGGCC TCACCGGCCG
    GAGTGGGTCC ATGACAAAGC CGACTACATG CCAGAGACCA GGCTGAGAAT CCCAGCAGCA
    GAGCCCATCG AGTACGCTCA GTTCCCTTTC TACCTCAACG GCCTACGAGA CACCTCAGAC
    TTTGTGGAAG CCATAGAAAA AGTGAGAGTC ATCTGTAACA ACTATACGAG CCTGGGACTG
    TCCAGCTACC CCAATGGCTA CCCCTTCCTG TTCTGGGAGC AATACATCAG CCTGCGCCAC
    TGGCTGCTGC TATCCATCAG CGTGGTGCTG GCCTGCACGT TTCTAGTGTG CGCAGTCTTC
    CTCCTGAACC CCTGGACGGC CGGGATCATT GTCATGGTCC TGGCTCTGAT GACCGTTGAG
    CTCTTTGGCA TGATGGGCCT CATTGGGATC AAGCTGAGTG CTGTGCCTGT GGTCATCCTG
    ATTGCATCTG TTGGCATCGG AGTGGAGTTC ACCGTCCACG TGGCTTTGGC CTTTCTGACA
    GCCATTGGGG ACAAGAACCA CAGGGCTATG CTCGCTCTGG AGCACATGTT TGCTCCCGTT
    CTGGACGGTG CTGTGTCCAC TCTGCTGGGT GTACTGATGC TTGCAGGGTC CGAATTTGAT
    TTCATTGTCA GATACTTCTT TGCCGTCCTG GCCATTCTCA CCGTCTTGGG GGTTCTCAAT
    GGACTGGTTC TGCTGCCTGT CCTCTTATCC TTCTTTGGAC CGTGTCCTGA GGTGTCTCCA
    GCCAATGGCC TAAACCGACT GCCCACTCCT TCGCCTGAGC CGCCTCCAAG TGTCGTCCGG
    TTTGCCGTGC CTCCTGGTCA CACGAACAAT GGGTCTGATT CCTCCGACTC GGAGTACAGC
    TCTCAGACCA CGGTGTCTGG CATCAGTGAG GAGCTCAGGC AATACGAAGC ACAGCAGGGT
    GCCGGAGGCC CTGCCCACCA AGTGATTGTG GAAGCCACAG AAAACCCTGT CTTTGCCCGG
    TCCACTGTGG TCCATCCGGA CTCCAGACAT CAGCCTCCCT TGACCCCTCG GCAACAGCCC
    CACCTGGACT CTGGCTCCTT GTCCCCTGGA CGGCAAGGCC AGCAGCCTCG AAGGGATCCC
    CCTAGAGAAG GCTTGCGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GACCGCTCAG GGCCCCGTGG GGCCCGTTCT
    CACAACCCTC GGAACCCAAC GTCCACCGCC ATGGGCAGCT CTGTGCCCAG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCTTCG GTGACTGTTG CTGTGCATCC CCCGCCTGGA
    CCTGGGCGCA ACCCCCGAGG GGGGCCCTGT CCAGGCTATG AGAGCTACCC TGAGACTGAT
    CACGGGGTAT TTGAGGATCC TCATGTGCCT TTTCATGTCA GGTGTGAGAG GAGGGACTCA
    AAGGTGGAGG TCATAGAGCT ACAGGACGTG GAATGTGAGG AGAGGCCGTG GGGGAGCAGC
    TCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22749
    Clone ID Related Accession (Same CDS sequence) NM_008957.3 , XM_006517159.3
    Accession Version NM_008957.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product protein patched homolog 1 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BF464755.1, AB164615.1, CK639136.1, AK138524.1, CF165613.1, CD545333.1, CK636036.1, CN531162.1, AK163736.1, CO433508.1, CB520013.1, AK147626.1 and BU700582.1. On Jun 16, 2016 this sequence version replaced NM_008957.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U46155.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
    AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC
    TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA
    GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT
    CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG
    GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA
    CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT
    CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT
    CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC
    AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT
    TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC
    TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA
    CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA
    GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG
    CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC
    AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT
    ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT
    GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA
    CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC
    ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC
    TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC
    TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT
    TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC
    CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT
    TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT
    GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG
    GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC
    AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC
    CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA
    ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC
    ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC
    AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC
    CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG
    TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG
    GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG
    TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG
    TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC
    CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG
    CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG
    TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT
    ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG
    GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA
    CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA
    AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC
    AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT
    AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC
    GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC
    TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC
    CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG
    AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC
    TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG
    GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG
    ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT
    GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG
    GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG
    GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG
    CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC
    GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC
    TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC
    ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG
    AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC
    AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG
    ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC
    AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC
    CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC
    CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC
    AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC
    ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT
    GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG
    CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT
    GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG
    GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032983.1
    CDS216..4520
    Translation

    Target ORF information:

    RefSeq Version NM_008957.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus patched 1 (Ptch1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008957.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
    AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC
    TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA
    GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT
    CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG
    GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA
    CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT
    CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT
    CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC
    AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT
    TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC
    TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA
    CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA
    GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG
    CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC
    AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT
    ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT
    GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA
    CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC
    ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC
    TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC
    TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT
    TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC
    CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT
    TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT
    GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG
    GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC
    AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC
    CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA
    ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC
    ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC
    AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC
    CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG
    TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG
    GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG
    TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG
    TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC
    CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG
    CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG
    TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT
    ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG
    GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA
    CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA
    AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC
    AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT
    AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC
    GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC
    TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC
    CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG
    AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC
    TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG
    GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG
    ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT
    GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG
    GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG
    GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG
    CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC
    GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC
    TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC
    ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG
    AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC
    AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG
    ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC
    AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC
    CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC
    CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC
    AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC
    ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT
    GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG
    CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT
    GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG
    GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22749
    Clone ID Related Accession (Same CDS sequence) NM_008957.3 , XM_006517159.3
    Accession Version XM_006517159.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein patched homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517159.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
    AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC
    TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA
    GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT
    CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG
    GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA
    CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT
    CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT
    CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC
    AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT
    TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC
    TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA
    CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA
    GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG
    CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC
    AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT
    ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT
    GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA
    CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC
    ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC
    TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC
    TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT
    TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC
    CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT
    TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT
    GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG
    GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC
    AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC
    CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA
    ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC
    ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC
    AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC
    CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG
    TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG
    GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG
    TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG
    TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC
    CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG
    CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG
    TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT
    ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG
    GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA
    CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA
    AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC
    AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT
    AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC
    GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC
    TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC
    CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG
    AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC
    TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG
    GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG
    ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT
    GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG
    GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG
    GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG
    CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC
    GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC
    TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC
    ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG
    AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC
    AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG
    ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC
    AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC
    CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC
    CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC
    AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC
    ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT
    GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG
    CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT
    GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG
    GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006517222.1
    CDS298..4602
    Translation

    Target ORF information:

    RefSeq Version XM_006517159.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus patched 1 (Ptch1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006517159.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGGTAACGC CGCCGGGGCC CTGGGCAGGC AGGCCGGCGG CGGGAGGCGC 
    AGACGGACCG GGGGACCGCA CCGCGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC
    TACTGCGACG CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA
    GCGCCGCTGT GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT
    CAAAAGAACT GCGGCAAGTT TTTGGTTGTG GGTCTCCTCA TATTTGGGGC CTTCGCTGTG
    GGATTAAAGG CAGCTAATCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGTGGA
    CGAGTGAGTC GAGAATTAAA TTATACCCGT CAGAAGATAG GAGAAGAGGC TATGTTTAAT
    CCTCAACTCA TGATACAGAC TCCAAAAGAA GAAGGCGCTA ATGTTCTGAC CACAGAGGCT
    CTCCTGCAAC ACCTGGACTC AGCACTCCAG GCCAGTCGTG TGCACGTCTA CATGTATAAC
    AGGCAATGGA AGTTGGAACA TTTGTGCTAC AAATCAGGGG AACTTATCAC GGAGACAGGT
    TACATGGATC AGATAATAGA ATACCTTTAC CCTTGCTTAA TCATTACACC TTTGGACTGC
    TTCTGGGAAG GGGCAAAGCT ACAGTCCGGG ACAGCATACC TCCTAGGTAA GCCTCCTTTA
    CGGTGGACAA ACTTTGACCC CTTGGAATTC CTAGAAGAGT TAAAGAAAAT AAACTACCAA
    GTGGACAGCT GGGAGGAAAT GCTGAATAAA GCCGAAGTTG GCCATGGGTA CATGGACCGG
    CCTTGCCTCA ACCCAGCCGA CCCAGATTGC CCTGCCACAG CCCCTAACAA AAATTCAACC
    AAACCTCTTG ATGTGGCCCT TGTTTTGAAT GGTGGATGTC AAGGTTTATC CAGGAAGTAT
    ATGCATTGGC AGGAGGAGTT GATTGTGGGT GGTACCGTCA AGAATGCCAC TGGAAAACTT
    GTCAGCGCTC ACGCCCTGCA AACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTATGAA
    CACTTCAGGG GCTACGACTA TGTCTCTCAC ATCAACTGGA ATGAAGACAG GGCAGCCGCC
    ATCCTGGAGG CCTGGCAGAG GACTTACGTG GAGGTGGTTC ATCAAAGTGT CGCCCCAAAC
    TCCACTCAAA AGGTGCTTCC CTTCACAACC ACGACCCTGG ACGACATCCT AAAATCCTTC
    TCTGATGTCA GTGTCATCCG AGTGGCCAGC GGCTACCTAC TGATGCTTGC CTATGCCTGT
    TTAACCATGC TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC
    CTGTTGGTTG CGCTGTCAGT GGCTGCAGGA TTGGGCCTCT GCTCCTTGAT TGGCATTTCT
    TTTAATGCTG CGACAACTCA GGTTTTGCCG TTTCTTGCTC TTGGTGTTGG TGTGGATGAT
    GTCTTCCTCC TGGCCCATGC ATTCAGTGAA ACAGGACAGA ATAAGAGGAT TCCATTTGAG
    GACAGGACTG GGGAGTGCCT CAAGCGCACC GGAGCCAGCG TGGCCCTCAC CTCCATCAGC
    AATGTCACCG CCTTCTTCAT GGCCGCATTG ATCCCTATCC CTGCCCTGCG AGCGTTCTCC
    CTCCAGGCTG CTGTGGTGGT GGTATTCAAT TTTGCTATGG TTCTGCTCAT TTTTCCTGCA
    ATTCTCAGCA TGGATTTATA CAGACGTGAG GACAGAAGAT TGGATATTTT CTGCTGTTTC
    ACAAGCCCCT GTGTCAGCAG GGTGATTCAA GTTGAGCCAC AGGCCTACAC AGAGCCTCAC
    AGTAACACCC GGTACAGCCC CCCACCCCCA TACACCAGCC ACAGCTTCGC CCACGAAACC
    CATATCACTA TGCAGTCCAC CGTTCAGCTC CGCACAGAGT ATGACCCTCA CACGCACGTG
    TACTACACCA CCGCCGAGCC ACGCTCTGAG ATCTCTGTAC AGCCTGTTAC CGTCACCCAG
    GACAACCTCA GCTGTCAGAG TCCCGAGAGC ACCAGCTCTA CCAGGGACCT GCTCTCCCAG
    TTCTCAGACT CCAGCCTCCA CTGCCTCGAG CCCCCCTGCA CCAAGTGGAC ACTCTCTTCG
    TTTGCAGAGA AGCACTATGC TCCTTTCCTC CTGAAACCCA AAGCCAAGGT TGTGGTAATC
    CTTCTTTTCC TGGGCTTGCT GGGGGTCAGC CTTTATGGGA CCACCCGAGT GAGAGACGGG
    CTGGACCTCA CGGACATTGT TCCCCGGGAA ACCAGAGAAT ATGACTTCAT AGCTGCCCAG
    TTCAAGTACT TCTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT
    ATCCAGCACC TACTTTACGA CCTTCATAAG AGTTTCAGCA ATGTGAAGTA TGTCATGCTG
    GAGGAGAACA AGCAACTTCC CCAAATGTGG CTGCACTACT TTAGAGACTG GCTTCAAGGA
    CTTCAGGATG CATTTGACAG TGACTGGGAA ACTGGGAGGA TCATGCCAAA CAATTATAAA
    AATGGATCAG ATGACGGGGT CCTCGCTTAC AAACTCCTGG TGCAGACTGG CAGCCGAGAC
    AAGCCCATCG ACATTAGTCA GTTGACTAAA CAGCGTCTGG TAGACGCAGA TGGCATCATT
    AATCCGAGCG CTTTCTACAT CTACCTGACC GCTTGGGTCA GCAACGACCC TGTAGCTTAC
    GCTGCCTCCC AGGCCAACAT CCGGCCTCAC CGGCCGGAGT GGGTCCATGA CAAAGCCGAC
    TACATGCCAG AGACCAGGCT GAGAATCCCA GCAGCAGAGC CCATCGAGTA CGCTCAGTTC
    CCTTTCTACC TCAACGGCCT ACGAGACACC TCAGACTTTG TGGAAGCCAT AGAAAAAGTG
    AGAGTCATCT GTAACAACTA TACGAGCCTG GGACTGTCCA GCTACCCCAA TGGCTACCCC
    TTCCTGTTCT GGGAGCAATA CATCAGCCTG CGCCACTGGC TGCTGCTATC CATCAGCGTG
    GTGCTGGCCT GCACGTTTCT AGTGTGCGCA GTCTTCCTCC TGAACCCCTG GACGGCCGGG
    ATCATTGTCA TGGTCCTGGC TCTGATGACC GTTGAGCTCT TTGGCATGAT GGGCCTCATT
    GGGATCAAGC TGAGTGCTGT GCCTGTGGTC ATCCTGATTG CATCTGTTGG CATCGGAGTG
    GAGTTCACCG TCCACGTGGC TTTGGCCTTT CTGACAGCCA TTGGGGACAA GAACCACAGG
    GCTATGCTCG CTCTGGAGCA CATGTTTGCT CCCGTTCTGG ACGGTGCTGT GTCCACTCTG
    CTGGGTGTAC TGATGCTTGC AGGGTCCGAA TTTGATTTCA TTGTCAGATA CTTCTTTGCC
    GTCCTGGCCA TTCTCACCGT CTTGGGGGTT CTCAATGGAC TGGTTCTGCT GCCTGTCCTC
    TTATCCTTCT TTGGACCGTG TCCTGAGGTG TCTCCAGCCA ATGGCCTAAA CCGACTGCCC
    ACTCCTTCGC CTGAGCCGCC TCCAAGTGTC GTCCGGTTTG CCGTGCCTCC TGGTCACACG
    AACAATGGGT CTGATTCCTC CGACTCGGAG TACAGCTCTC AGACCACGGT GTCTGGCATC
    AGTGAGGAGC TCAGGCAATA CGAAGCACAG CAGGGTGCCG GAGGCCCTGC CCACCAAGTG
    ATTGTGGAAG CCACAGAAAA CCCTGTCTTT GCCCGGTCCA CTGTGGTCCA TCCGGACTCC
    AGACATCAGC CTCCCTTGAC CCCTCGGCAA CAGCCCCACC TGGACTCTGG CTCCTTGTCC
    CCTGGACGGC AAGGCCAGCA GCCTCGAAGG GATCCCCCTA GAGAAGGCTT GCGGCCACCC
    CCCTACAGAC CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC
    AATAGGGACC GCTCAGGGCC CCGTGGGGCC CGTTCTCACA ACCCTCGGAA CCCAACGTCC
    ACCGCCATGG GCAGCTCTGT GCCCAGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT
    GCTTCGGTGA CTGTTGCTGT GCATCCCCCG CCTGGACCTG GGCGCAACCC CCGAGGGGGG
    CCCTGTCCAG GCTATGAGAG CTACCCTGAG ACTGATCACG GGGTATTTGA GGATCCTCAT
    GTGCCTTTTC ATGTCAGGTG TGAGAGGAGG GACTCAAAGG TGGAGGTCAT AGAGCTACAG
    GACGTGGAAT GTGAGGAGAG GCCGTGGGGG AGCAGCTCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.