Tmprss15 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmprss15
Entrez Gene ID	19146
Full Name	transmembrane protease, serine 15
Synonyms	Entk,Prss7
General protein information	Preferred Names transmembrane protease, serine 15 Names enteropeptidase enterokinase protease, serine, 7 (enterokinase)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.1-C3.2
Summary	This gene encodes an enzyme that proteolytically activates the pancreatic proenzyme trypsinogen, converting it into trypsin. The encoded protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008941.3	NP_032967.1	enteropeptidase isoform 1 precursor
NM_178855.4	NP_849186.2	enteropeptidase isoform 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMPRSS15	NP_002763.2
Bos taurus (cattle)	TMPRSS15	NP_776864.1
	TMPRSS15	XP_002803225.1
Rattus norvegicus (Norway rat)	Tmprss15	NP_001099365.1
Gallus gallus (chicken)	TMPRSS15	XP_425539.4
Canis lupus familiaris (dog)	TMPRSS15	XP_544824.2
Pan troglodytes (chimpanzee)	TMPRSS15	XP_514836.2
Danio rerio (zebrafish)	tmprss15	XP_001919639.2
Xenopus tropicalis (tropical clawed frog)	tmprss15	XP_002933130.2
Mus musculus (house mouse)	Tmprss15	NP_849186.2

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition effect of enteropeptidase on RANKL-RANK signalling by cleavage of RANK.
Zhao Y, Jin M, Ma J, Zhang S, Li W, Chen Y, Zhou Y, Tao H, Liu Y, Wang L, Han H, Niu G, Tao H, Liu C, Gao B
FEBS letters587(18)2958-64(2013 Sep)

Gene expression profiling in a mouse model for African trypanosomiasis.
Kierstein S, Noyes H, Naessens J, Nakamura Y, Pritchard C, Gibson J, Kemp S, Brass A
Genes and immunity7(8)667-79(2006 Dec)

Structure of murine enterokinase (enteropeptidase) and expression in small intestine during development.
Yuan X, Zheng X, Lu D, Rubin DC, Pung CY, Sadler JE
The American journal of physiology274(2 Pt 1)G342-9(1998 Feb)

Two-dimensional gel analysis of zymogen-activating factors in the small intestine of the mouse.
Isobe M, Ogita Z
The Journal of experimental zoology231(1)19-26(1984 Jul)

Electrophoretic analysis of pancreatic proteases and zymogen-activating factors in the mouse.
Isobe M, Ogita Z
The Journal of experimental zoology230(3)347-54(1984 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Enteropeptidase could inhibit osteoclastogenesis in vitro through the cleavage of RANK.
Title: Inhibition effect of enteropeptidase on RANKL-RANK signalling by cleavage of RANK.

peptide sequencing of amino terminus of light chain of cow enterokinase, identifying the cleavage site
Title: The amino-terminal sequence of the catalytic subunit of bovine enterokinase.

The following Tmprss15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmprss15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03132	NM_008941.3 Latest version!	Mus musculus transmembrane protease, serine 15 (Tmprss15), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu07234	NM_178855.4 Latest version!	Mus musculus transmembrane protease, serine 15 (Tmprss15), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmprss15 ( NM_008941.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032967.1 Mus musculus transmembrane protease, serine 15 (Tmprss15), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03132
Clone ID Related Accession (Same CDS sequence)	NM_008941.3
Accession Version	NM_008941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	enteropeptidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK033582.1 and U73378.1. On Jan 16, 2013 this sequence version replaced NM_008941.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: U73378.1, BC117917.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849378 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATCCA GCAGAGACGA GGCTGTAGGA CATCACTCTA TCAGCTCTTT TGAAGTGATG 
CTCTCAGCTC TCTTCATTAT GCTCATGGTC TTCTCTATTG GATTAATTGC AGTGTCCTGG 
CTTGCAGTCA AGGAATCTGA AGGAGATGCA GCCCTGGGGA AAAGTCATGA AGTCAGAGGG 
ACATTTAAAA TAACATCTGG AGTGACATAT AATCCTAATT TACAAGACAA ACACTCTGTG 
GATTTCAAAG TTCTTGCTTT TGACCTTCAG CAAATGATAG ACGAGATCTT TGAATCAAGT 
AGTCTGAAGA ATGAATATGA AAAATCGAAG GTTTTTCAAT TTGAAAAAGG CAGCGTTATT 
GTCCTCTTTG ACCTCTTTTT TGCTCAGTGG GTGTCAGATA AAAATGTAAA AGAAGAACTG 
ATTCAGGGCA TTGAAGCAAA TATATCCAGC CAACTGGTCA CTCTCCATAT TGATTTGAAC 
AGCATTGATA TCACAGCATC CTTGAGTGAC TTCACAACAG CAGTCCCTGT AACAACTTCA 
GATAAGCTAA CAACCAGCAG TCCTATGACA ACTTCAGCAT CACTGGGTAA CTTATCAACA 
ACAGTGGCTG CAACAACTTC AGCACCATTG TGTAATTTAT CAACAGCAAC TTTTGCAACA 
ACTTCAGGCC ATGTCTCAAT AGAGTGCCAG CCTGGTTCAC GGCCCTGTGC TCATGCTTGG 
AACTGTGTAG CAACTGATTT GTTTTGTGAT GGAGAAGTGA ACTGTCCAGA TGGTTCAGAT 
GAAGACACTG GGCTATGTGC CACAGCTTGT GATGGAAGAT TTTTGTTGAC TGGAGATTCT 
GGGGTTTTCC AAGCTGATCG TTATCCAAGG CCAGATGAAT CAGGTGTTGT TTGCCGATGG 
ATCATCCGTG TAAACCAAGG ACTTTCCATT AGAATGAACT TTGGATCTTT TATTCCACAT 
TATACAGATG TGTTAGATAT CTATGAAGGT ATAGGACCTA GCAAAATTTT AAGAGGCTCC 
TTTTGGGAAA CTGATCCTGG AACAATTCGA ATTTTTTCCA ACCTAGTTAC TGTCACCTTT 
CTGATAAAAT CAGATGAATA TGATTACATT GGTTTTAATG CAACGTACAG CACTTTCAAC 
AACAGCGAAC TTAACAATTA TGAGAAAATC GACTGTACAT TTGATGATGG ATTTTGCTTC 
TGGACCCAGG ATCTTGATGA TGATAATGAG TGGGAAAGAA TCCAAGTGAC CACGTTTCCT 
TGCTACACTG GCCCACGTTT TGACCACACA TATGGGAATG GCTCAGGATT TTACATTTCT 
ACACCAACTG AACAGGGATG GAGAAGCGAA AGAGTAGGCC TTTCAAGCCT CTCTTTAGAC 
CTCACTTCAG AGCCTGTCTG CCTTCATTTT TGGTATTACA TGTGCTGTGA AAATGTCTAC 
AATTTAAACA TTCATATCAG CAGTGCTGAA ACCACAGACA AGATAGTTTT TCAAAGGAAA 
GGAAACTATG GGAGGAACTG GAATTATGGT CAAGTCACAT TAAATGAAAC AGGAGAATTT 
AAGGTTGTGT TCAATGCTTT CAGAAACCGA GGTTGCAGTA CAATAGCCCT GGATGATATA 
AGCCTAACAA ATGGAATTTG TAGTCAGAGT CCTTATCCAG AACCAACCTT GGTTCCCACC 
CCTCCACCAG AGCTTCCAAC TGACTGCGGA GGACCTTTTG AACTATGGGA GCCAAATTCA 
ACCTTCAGTT CTCCGAACTT TCCAGACAAA TATCCCAATC AAGCTTCCTG TATATGGAAT 
TTAAATGCAC AGAGAGGGAA AAATATTCAA CTTCATTTTC AAGAATTCGA TTTAGAAAAC 
ATCAATGATG TGGTGGAAGT TAGAGATGGG GGAGAATTTG ACTCCCTGCT CCTAGCTGTG 
TACACAGGAC CTGGTCCTGT GAAGGACTTG TTCTCTACCA CCAATAGGAT GACTGTGATT 
TTCACCACAA ACATGGAGAC CAGACGCAAG GGCTTCAAAG CAAATTTCAC TTCCGGCTAT 
TACTTGGGGA TTCCAGAGCC CTGCCAAGAT GACGAATTTC AGTGCAAAGA TGGAAACTGC 
ATTCCTCTGG GGAATCTCTG TGATAGCTAC CCGCACTGTA GAGATGGCTC AGATGAAGCA 
AGCTGTGTGC GTTTTCTTAA TGGCACAAGA AGCAACAATG GGTTGGTACA GTTCAATATC 
CATAGCATAT GGCATATAGC TTGTGCTGAG AACTGGACCA CACAAATTTC AAATGAAGTT 
TGTCATCTTC TGGGACTAGG GAGTGCAAAC TCATCAATGC CAATATCATC TACCGGAGGT 
GGACCATTTG TGAGAGTAAA CCAAGCCCCT AATGGAAGCT TGATACTAAC TCCTAGTCTA 
CAATGCTCCC AAGATTCCTT GATTCTATTG CAATGCAACC ATAAATCATG TGGAGAAAAA 
AAGGTGACTC AGAAAGTCAG CCCAAAGATC GTTGGTGGAA GTGACGCCCA GGCAGGGGCC 
TGGCCCTGGG TTGTGGCTTT GTATCACAGA GACAGGTCTA CAGACCGTCT GCTCTGCGGT 
GCATCTCTTG TCAGCAGTGA CTGGCTGGTG TCAGCTGCAC ACTGTGTGTA TAGAAGAAAT 
CTAGATCCAA CCAGATGGAC AGCAGTCCTG GGCCTGCATA TGCAATCAAA TCTGACTTCT 
CCTCAAGTAG TAAGACGGGT GGTTGATCAA ATTGTCATAA ACCCTCATTA TGACAGACGC 
AGAAAGGTCA ACGACATCGC TATGATGCAC CTTGAGTTTA AAGTAAATTA CACAGATTAT 
ATACAGCCTA TTTGTTTACC AGAAGAGAAT CAAATATTTA TTCCAGGAAG AACTTGTTCG 
ATTGCTGGCT GGGGATATGA TAAAATAAAT GCAGGTTCTA CTGTTGATGT GCTGAAAGAA 
GCTGATGTTC CTCTTATATC CAATGAAAAG TGCCAGCAAC AGCTGCCAGA ATACAACATC 
ACTGAAAGTA TGATATGTGC AGGATATGAA GAAGGAGGAA TAGATTCTTG TCAGGGTGAT 
TCTGGAGGGC CATTAATGTG CCAAGAAAAC AACAGATGGT TTCTTGTTGG TGTGACATCA 
TTTGGAGTCC AGTGTGCACT GCCTAATCAC CCTGGGGTAT ATGTCCGAGT CTCACAGTTT 
ATAGAATGGA TCCATAGCTT TCTACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032967.1
CDS	181..3390
Translation	MKSSRDEAVGHHSISSFEVMLSALFIMLMVFSIGLIAVSWLAVKESEGDAALGKSHEVRGTFKITSGVTYNPNLQDKHSVDFKVLAFDLQQMIDEIFESSSLKNEYEKSKVFQFEKGSVIVLFDLFFAQWVSDKNVKEELIQGIEANISSQLVTLHIDLNSIDITASLSDFTTAVPVTTSDKLTTSSPMTTSASLGNLSTTVAATTSAPLCNLSTATFATTSGHVSIECQPGSRPCAHAWNCVATDLFCDGEVNCPDGSDEDTGLCATACDGRFLLTGDSGVFQADRYPRPDESGVVCRWIIRVNQGLSIRMNFGSFIPHYTDVLDIYEGIGPSKILRGSFWETDPGTIRIFSNLVTVTFLIKSDEYDYIGFNATYSTFNNSELNNYEKIDCTFDDGFCFWTQDLDDDNEWERIQVTTFPCYTGPRFDHTYGNGSGFYISTPTEQGWRSERVGLSSLSLDLTSEPVCLHFWYYMCCENVYNLNIHISSAETTDKIVFQRKGNYGRNWNYGQVTLNETGEFKVVFNAFRNRGCSTIALDDISLTNGICSQSPYPEPTLVPTPPPELPTDCGGPFELWEPNSTFSSPNFPDKYPNQASCIWNLNAQRGKNIQLHFQEFDLENINDVVEVRDGGEFDSLLLAVYTGPGPVKDLFSTTNRMTVIFTTNMETRRKGFKANFTSGYYLGIPEPCQDDEFQCKDGNCIPLGNLCDSYPHCRDGSDEASCVRFLNGTRSNNGLVQFNIHSIWHIACAENWTTQISNEVCHLLGLGSANSSMPISSTGGGPFVRVNQAPNGSLILTPSLQCSQDSLILLQCNHKSCGEKKVTQKVSPKIVGGSDAQAGAWPWVVALYHRDRSTDRLLCGASLVSSDWLVSAAHCVYRRNLDPTRWTAVLGLHMQSNLTSPQVVRRVVDQIVINPHYDRRRKVNDIAMMHLEFKVNYTDYIQPICLPEENQIFIPGRTCSIAGWGYDKINAGSTVDVLKEADVPLISNEKCQQQLPEYNITESMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLVGVTSFGVQCALPNHPGVYVRVSQFIEWIHSFLH

Target ORF information:

RefSeq Version	NM_008941.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protease, serine 15 (Tmprss15), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008941.3

ORF Insert Sequence:

ATGAAATCCA GCAGAGACGA GGCTGTAGGA CATCACTCTA TCAGCTCTTT TGAAGTGATG 
CTCTCAGCTC TCTTCATTAT GCTCATGGTC TTCTCTATTG GATTAATTGC AGTGTCCTGG 
CTTGCAGTCA AGGAATCTGA AGGAGATGCA GCCCTGGGGA AAAGTCATGA AGTCAGAGGG 
ACATTTAAAA TAACATCTGG AGTGACATAT AATCCTAATT TACAAGACAA ACACTCTGTG 
GATTTCAAAG TTCTTGCTTT TGACCTTCAG CAAATGATAG ACGAGATCTT TGAATCAAGT 
AGTCTGAAGA ATGAATATGA AAAATCGAAG GTTTTTCAAT TTGAAAAAGG CAGCGTTATT 
GTCCTCTTTG ACCTCTTTTT TGCTCAGTGG GTGTCAGATA AAAATGTAAA AGAAGAACTG 
ATTCAGGGCA TTGAAGCAAA TATATCCAGC CAACTGGTCA CTCTCCATAT TGATTTGAAC 
AGCATTGATA TCACAGCATC CTTGAGTGAC TTCACAACAG CAGTCCCTGT AACAACTTCA 
GATAAGCTAA CAACCAGCAG TCCTATGACA ACTTCAGCAT CACTGGGTAA CTTATCAACA 
ACAGTGGCTG CAACAACTTC AGCACCATTG TGTAATTTAT CAACAGCAAC TTTTGCAACA 
ACTTCAGGCC ATGTCTCAAT AGAGTGCCAG CCTGGTTCAC GGCCCTGTGC TCATGCTTGG 
AACTGTGTAG CAACTGATTT GTTTTGTGAT GGAGAAGTGA ACTGTCCAGA TGGTTCAGAT 
GAAGACACTG GGCTATGTGC CACAGCTTGT GATGGAAGAT TTTTGTTGAC TGGAGATTCT 
GGGGTTTTCC AAGCTGATCG TTATCCAAGG CCAGATGAAT CAGGTGTTGT TTGCCGATGG 
ATCATCCGTG TAAACCAAGG ACTTTCCATT AGAATGAACT TTGGATCTTT TATTCCACAT 
TATACAGATG TGTTAGATAT CTATGAAGGT ATAGGACCTA GCAAAATTTT AAGAGGCTCC 
TTTTGGGAAA CTGATCCTGG AACAATTCGA ATTTTTTCCA ACCTAGTTAC TGTCACCTTT 
CTGATAAAAT CAGATGAATA TGATTACATT GGTTTTAATG CAACGTACAG CACTTTCAAC 
AACAGCGAAC TTAACAATTA TGAGAAAATC GACTGTACAT TTGATGATGG ATTTTGCTTC 
TGGACCCAGG ATCTTGATGA TGATAATGAG TGGGAAAGAA TCCAAGTGAC CACGTTTCCT 
TGCTACACTG GCCCACGTTT TGACCACACA TATGGGAATG GCTCAGGATT TTACATTTCT 
ACACCAACTG AACAGGGATG GAGAAGCGAA AGAGTAGGCC TTTCAAGCCT CTCTTTAGAC 
CTCACTTCAG AGCCTGTCTG CCTTCATTTT TGGTATTACA TGTGCTGTGA AAATGTCTAC 
AATTTAAACA TTCATATCAG CAGTGCTGAA ACCACAGACA AGATAGTTTT TCAAAGGAAA 
GGAAACTATG GGAGGAACTG GAATTATGGT CAAGTCACAT TAAATGAAAC AGGAGAATTT 
AAGGTTGTGT TCAATGCTTT CAGAAACCGA GGTTGCAGTA CAATAGCCCT GGATGATATA 
AGCCTAACAA ATGGAATTTG TAGTCAGAGT CCTTATCCAG AACCAACCTT GGTTCCCACC 
CCTCCACCAG AGCTTCCAAC TGACTGCGGA GGACCTTTTG AACTATGGGA GCCAAATTCA 
ACCTTCAGTT CTCCGAACTT TCCAGACAAA TATCCCAATC AAGCTTCCTG TATATGGAAT 
TTAAATGCAC AGAGAGGGAA AAATATTCAA CTTCATTTTC AAGAATTCGA TTTAGAAAAC 
ATCAATGATG TGGTGGAAGT TAGAGATGGG GGAGAATTTG ACTCCCTGCT CCTAGCTGTG 
TACACAGGAC CTGGTCCTGT GAAGGACTTG TTCTCTACCA CCAATAGGAT GACTGTGATT 
TTCACCACAA ACATGGAGAC CAGACGCAAG GGCTTCAAAG CAAATTTCAC TTCCGGCTAT 
TACTTGGGGA TTCCAGAGCC CTGCCAAGAT GACGAATTTC AGTGCAAAGA TGGAAACTGC 
ATTCCTCTGG GGAATCTCTG TGATAGCTAC CCGCACTGTA GAGATGGCTC AGATGAAGCA 
AGCTGTGTGC GTTTTCTTAA TGGCACAAGA AGCAACAATG GGTTGGTACA GTTCAATATC 
CATAGCATAT GGCATATAGC TTGTGCTGAG AACTGGACCA CACAAATTTC AAATGAAGTT 
TGTCATCTTC TGGGACTAGG GAGTGCAAAC TCATCAATGC CAATATCATC TACCGGAGGT 
GGACCATTTG TGAGAGTAAA CCAAGCCCCT AATGGAAGCT TGATACTAAC TCCTAGTCTA 
CAATGCTCCC AAGATTCCTT GATTCTATTG CAATGCAACC ATAAATCATG TGGAGAAAAA 
AAGGTGACTC AGAAAGTCAG CCCAAAGATC GTTGGTGGAA GTGACGCCCA GGCAGGGGCC 
TGGCCCTGGG TTGTGGCTTT GTATCACAGA GACAGGTCTA CAGACCGTCT GCTCTGCGGT 
GCATCTCTTG TCAGCAGTGA CTGGCTGGTG TCAGCTGCAC ACTGTGTGTA TAGAAGAAAT 
CTAGATCCAA CCAGATGGAC AGCAGTCCTG GGCCTGCATA TGCAATCAAA TCTGACTTCT 
CCTCAAGTAG TAAGACGGGT GGTTGATCAA ATTGTCATAA ACCCTCATTA TGACAGACGC 
AGAAAGGTCA ACGACATCGC TATGATGCAC CTTGAGTTTA AAGTAAATTA CACAGATTAT 
ATACAGCCTA TTTGTTTACC AGAAGAGAAT CAAATATTTA TTCCAGGAAG AACTTGTTCG 
ATTGCTGGCT GGGGATATGA TAAAATAAAT GCAGGTTCTA CTGTTGATGT GCTGAAAGAA 
GCTGATGTTC CTCTTATATC CAATGAAAAG TGCCAGCAAC AGCTGCCAGA ATACAACATC 
ACTGAAAGTA TGATATGTGC AGGATATGAA GAAGGAGGAA TAGATTCTTG TCAGGGTGAT 
TCTGGAGGGC CATTAATGTG CCAAGAAAAC AACAGATGGT TTCTTGTTGG TGTGACATCA 
TTTGGAGTCC AGTGTGCACT GCCTAATCAC CCTGGGGTAT ATGTCCGAGT CTCACAGTTT 
ATAGAATGGA TCCATAGCTT TCTACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmprss15 ( NM_178855.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_849186.2 Mus musculus transmembrane protease, serine 15 (Tmprss15), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07234
Clone ID Related Accession (Same CDS sequence)	NM_178855.4
Accession Version	NM_178855.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	enteropeptidase isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK033582.1 and U73378.1. On Jan 16, 2013 this sequence version replaced NM_178855.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK033582.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849378 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATCCA GCAGAGACGA GGCTGTAGGA CATCACTCTA TCAGCTCTTT TGAAGTGATG 
CTCTCAGCTC TCTTCATTAT GCTCATGGTC TTCTCTATTG GATTAATTGC AGTGTCCTGG 
CTTGCAGTCA AGGAATCTGA AGGAGATGCA GCCCTGGGGA AAAGTCATGA AGTCAGAGGG 
ACATTTAAAA TAACATCTGG AGTGACATAT AATCCTAATT TACAAGACAA ACACTCTGTG 
GATTTCAAAG TTCTTGCTTT TGACCTTCAG CAAATGATAG ACGAGATCTT TGAATCAAGT 
AGTCTGAAGA ATGAATATGA AAAATCGAAG GTTTTTCAAT TTGAAAAAGG CAGCGTTATT 
GTCCTCTTTG ACCTCTTTTT TGCTCAGTGG GTGTCAGATA AAAATGTAAA AGAAGAACTG 
ATTCAGGGCA TTGAAGCAAA TATATCCAGC CAACTGGTCA CTCTCCATAT TGATTTGAAC 
AGCATTGATA TCACAGCATC CTTGAGTGAC TTCACAACAG CAGTCCCTGT AACAACTTCA 
GATAAGCTAA CAACCAGCAG TCCTATGACA ACTTCAGCAT CACTGGGTAA CTTATCAACA 
ACAGTGGCTG CAACAACTTC AGGCCATGTC TCAATAGAGT GCCAGCCTGG TTCACGGCCC 
TGTGCTCATG CTTGGAACTG TGTAGCAACT GATTTGTTTT GTGATGGAGA AGTGAACTGT 
CCAGATGGTT CAGATGAAGA CACTGGGCTA TGTGCCACAG CTTGTGATGG AAGATTTTTG 
TTGACTGGAG ATTCTGGGGT TTTCCAAGCT GATCGTTATC CAAGGCCAGA TGAATCAGGT 
GTTGTTTGCC GATGGATCAT CCGTGTAAAC CAAGGACTTT CCATTAGAAT GAACTTTGGA 
TCTTTTATTC CACATTATAC AGATGTGTTA GATATCTATG AAGGTATAGG ACCTAGCAAA 
ATTTTAAGAG GCTCCTTTTG GGAAACTGAT CCTGGAACAA TTCGAATTTT TTCCAACCTA 
GTTACTGTCA CCTTTCTGAT AAAATCAGAT GAATATGATT ACATTGGTTT TAATGCAACG 
TACAGCACTT TCAACAACAG CGAACTTAAC AATTATGAGA AAATCGACTG TACATTTGAT 
GATGGATTTT GCTTCTGGAC CCAGGATCTT GATGATGATA ATGAGTGGGA AAGAATCCAA 
GTGACCACGT TTCCTTGCTA CACTGGCCCA CGTTTTGACC ACACATATGG GAATGGCTCA 
GGATTTTACA TTTCTACACC AACTGAACAG GGATGGAGAA GCGAAAGAGT AGGCCTTTCA 
AGCCTCTCTT TAGACCTCAC TTCAGAGCCT GTCTGCCTTC ATTTTTGGTA TTACATGTGC 
TGTGAAAATG TCTACAATTT AAACATTCAT ATCAGCAGTG CTGAAACCAC AGACAAGATA 
GTTTTTCAAA GGAAAGGAAA CTATGGGAGG AACTGGAATT ATGGTCAAGT CACATTAAAT 
GAAACAGGAG AATTTAAGGT TGTGTTCAAT GCTTTCAGAA ACCGAGGTTG CAGTACAATA 
GCCCTGGATG ATATAAGCCT AACAAATGGA ATTTGTAGTC AGAGTCCTTA TCCAGAACCA 
ACCTTGGTTC CCACCCCTCC ACCAGAGCTT CCAACTGACT GCGGAGGACC TTTTGAACTA 
TGGGAGCCAA ATTCAACCTT CAGTTCTCCG AACTTTCCAG ACAAATATCC CAATCAAGCT 
TCCTGTATAT GGAATTTAAA TGCACAGAGA GGGAAAAATA TTCAACTTCA TTTTCAAGAA 
TTCGATTTAG AAAACATCAA TGATGTGGTG GAAGTTAGAG ATGGGGGAGA ATTTGACTCC 
CTGCTCCTAG CTGTGTACAC AGGACCTGGT CCTGTGAAGG ACTTGTTCTC TACCACCAAT 
AGGATGACTG TGATTTTCAC CACAAACATG GAGACCAGAC GCAAGGGCTT CAAAGCAAAT 
TTCACTTCCG GCTATTACTT GGGGATTCCA GAGCCCTGCC AAGATGACGA ATTTCAGTGC 
AAAGATGGAA ACTGCATTCC TCTGGGGAAT CTCTGTGATA GCTACCCGCA CTGTAGAGAT 
GGCTCAGATG AAGCAAGCTG TGTGCGTTTT CTTAATGGCA CAAGAAGCAA CAATGGGTTG 
GTACAGTTCA ATATCCATAG CATATGGCAT ATAGCTTGTG CTGAGAACTG GACCACACAA 
ATTTCAAATG AAGTTTGTCA TCTTCTGGGA CTAGGGAGTG CAAACTCATC AATGCCAATA 
TCATCTACCG GAGGTGGACC ATTTGTGAGA GTAAACCAAG CCCCTAATGG AAGCTTGATA 
CTAACTCCTA GTCTACAATG CTCCCAAGAT TCCTTGATTC TATTGCAATG CAACCATAAA 
TCATGTGGAG AAAAAAAGGT GACTCAGAAA GTCAGCCCAA AGATCGTTGG TGGAAGTGAC 
GCCCAGGCAG GGGCCTGGCC CTGGGTTGTG GCTTTGTATC ACAGAGACAG GTCTACAGAC 
CGTCTGCTCT GCGGTGCATC TCTTGTCAGC AGTGACTGGC TGGTGTCAGC TGCACACTGT 
GTGTATAGAA GAAATCTAGA TCCAACCAGA TGGACAGCAG TCCTGGGCCT GCATATGCAA 
TCAAATCTGA CTTCTCCTCA AGTAGTAAGA CGGGTGGTTG ATCAAATTGT CATAAACCCT 
CATTATGACA GACGCAGAAA GGTCAACGAC ATCGCTATGA TGCACCTTGA GTTTAAAGTA 
AATTACACAG ATTATATACA GCCTATTTGT TTACCAGAAG AGAATCAAAT ATTTATTCCA 
GGAAGAACTT GTTCGATTGC TGGCTGGGGA TATGATAAAA TAAATGCAGG TTCTACTGTT 
GATGTGCTGA AAGAAGCTGA TGTTCCTCTT ATATCCAATG AAAAGTGCCA GCAACAGCTG 
CCAGAATACA ACATCACTGA AAGTATGATA TGTGCAGGAT ATGAAGAAGG AGGAATAGAT 
TCTTGTCAGG GTGATTCTGG AGGGCCATTA ATGTGCCAAG AAAACAACAG ATGGTTTCTT 
GTTGGTGTGA CATCATTTGG AGTCCAGTGT GCACTGCCTA ATCACCCTGG GGTATATGTC 
CGAGTCTCAC AGTTTATAGA ATGGATCCAT AGCTTTCTAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849186.2
CDS	181..3345
Translation	MKSSRDEAVGHHSISSFEVMLSALFIMLMVFSIGLIAVSWLAVKESEGDAALGKSHEVRGTFKITSGVTYNPNLQDKHSVDFKVLAFDLQQMIDEIFESSSLKNEYEKSKVFQFEKGSVIVLFDLFFAQWVSDKNVKEELIQGIEANISSQLVTLHIDLNSIDITASLSDFTTAVPVTTSDKLTTSSPMTTSASLGNLSTTVAATTSGHVSIECQPGSRPCAHAWNCVATDLFCDGEVNCPDGSDEDTGLCATACDGRFLLTGDSGVFQADRYPRPDESGVVCRWIIRVNQGLSIRMNFGSFIPHYTDVLDIYEGIGPSKILRGSFWETDPGTIRIFSNLVTVTFLIKSDEYDYIGFNATYSTFNNSELNNYEKIDCTFDDGFCFWTQDLDDDNEWERIQVTTFPCYTGPRFDHTYGNGSGFYISTPTEQGWRSERVGLSSLSLDLTSEPVCLHFWYYMCCENVYNLNIHISSAETTDKIVFQRKGNYGRNWNYGQVTLNETGEFKVVFNAFRNRGCSTIALDDISLTNGICSQSPYPEPTLVPTPPPELPTDCGGPFELWEPNSTFSSPNFPDKYPNQASCIWNLNAQRGKNIQLHFQEFDLENINDVVEVRDGGEFDSLLLAVYTGPGPVKDLFSTTNRMTVIFTTNMETRRKGFKANFTSGYYLGIPEPCQDDEFQCKDGNCIPLGNLCDSYPHCRDGSDEASCVRFLNGTRSNNGLVQFNIHSIWHIACAENWTTQISNEVCHLLGLGSANSSMPISSTGGGPFVRVNQAPNGSLILTPSLQCSQDSLILLQCNHKSCGEKKVTQKVSPKIVGGSDAQAGAWPWVVALYHRDRSTDRLLCGASLVSSDWLVSAAHCVYRRNLDPTRWTAVLGLHMQSNLTSPQVVRRVVDQIVINPHYDRRRKVNDIAMMHLEFKVNYTDYIQPICLPEENQIFIPGRTCSIAGWGYDKINAGSTVDVLKEADVPLISNEKCQQQLPEYNITESMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLVGVTSFGVQCALPNHPGVYVRVSQFIEWIHSFLH

Target ORF information:

RefSeq Version	NM_178855.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protease, serine 15 (Tmprss15), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178855.4

ORF Insert Sequence:

ATGAAATCCA GCAGAGACGA GGCTGTAGGA CATCACTCTA TCAGCTCTTT TGAAGTGATG 
CTCTCAGCTC TCTTCATTAT GCTCATGGTC TTCTCTATTG GATTAATTGC AGTGTCCTGG 
CTTGCAGTCA AGGAATCTGA AGGAGATGCA GCCCTGGGGA AAAGTCATGA AGTCAGAGGG 
ACATTTAAAA TAACATCTGG AGTGACATAT AATCCTAATT TACAAGACAA ACACTCTGTG 
GATTTCAAAG TTCTTGCTTT TGACCTTCAG CAAATGATAG ACGAGATCTT TGAATCAAGT 
AGTCTGAAGA ATGAATATGA AAAATCGAAG GTTTTTCAAT TTGAAAAAGG CAGCGTTATT 
GTCCTCTTTG ACCTCTTTTT TGCTCAGTGG GTGTCAGATA AAAATGTAAA AGAAGAACTG 
ATTCAGGGCA TTGAAGCAAA TATATCCAGC CAACTGGTCA CTCTCCATAT TGATTTGAAC 
AGCATTGATA TCACAGCATC CTTGAGTGAC TTCACAACAG CAGTCCCTGT AACAACTTCA 
GATAAGCTAA CAACCAGCAG TCCTATGACA ACTTCAGCAT CACTGGGTAA CTTATCAACA 
ACAGTGGCTG CAACAACTTC AGGCCATGTC TCAATAGAGT GCCAGCCTGG TTCACGGCCC 
TGTGCTCATG CTTGGAACTG TGTAGCAACT GATTTGTTTT GTGATGGAGA AGTGAACTGT 
CCAGATGGTT CAGATGAAGA CACTGGGCTA TGTGCCACAG CTTGTGATGG AAGATTTTTG 
TTGACTGGAG ATTCTGGGGT TTTCCAAGCT GATCGTTATC CAAGGCCAGA TGAATCAGGT 
GTTGTTTGCC GATGGATCAT CCGTGTAAAC CAAGGACTTT CCATTAGAAT GAACTTTGGA 
TCTTTTATTC CACATTATAC AGATGTGTTA GATATCTATG AAGGTATAGG ACCTAGCAAA 
ATTTTAAGAG GCTCCTTTTG GGAAACTGAT CCTGGAACAA TTCGAATTTT TTCCAACCTA 
GTTACTGTCA CCTTTCTGAT AAAATCAGAT GAATATGATT ACATTGGTTT TAATGCAACG 
TACAGCACTT TCAACAACAG CGAACTTAAC AATTATGAGA AAATCGACTG TACATTTGAT 
GATGGATTTT GCTTCTGGAC CCAGGATCTT GATGATGATA ATGAGTGGGA AAGAATCCAA 
GTGACCACGT TTCCTTGCTA CACTGGCCCA CGTTTTGACC ACACATATGG GAATGGCTCA 
GGATTTTACA TTTCTACACC AACTGAACAG GGATGGAGAA GCGAAAGAGT AGGCCTTTCA 
AGCCTCTCTT TAGACCTCAC TTCAGAGCCT GTCTGCCTTC ATTTTTGGTA TTACATGTGC 
TGTGAAAATG TCTACAATTT AAACATTCAT ATCAGCAGTG CTGAAACCAC AGACAAGATA 
GTTTTTCAAA GGAAAGGAAA CTATGGGAGG AACTGGAATT ATGGTCAAGT CACATTAAAT 
GAAACAGGAG AATTTAAGGT TGTGTTCAAT GCTTTCAGAA ACCGAGGTTG CAGTACAATA 
GCCCTGGATG ATATAAGCCT AACAAATGGA ATTTGTAGTC AGAGTCCTTA TCCAGAACCA 
ACCTTGGTTC CCACCCCTCC ACCAGAGCTT CCAACTGACT GCGGAGGACC TTTTGAACTA 
TGGGAGCCAA ATTCAACCTT CAGTTCTCCG AACTTTCCAG ACAAATATCC CAATCAAGCT 
TCCTGTATAT GGAATTTAAA TGCACAGAGA GGGAAAAATA TTCAACTTCA TTTTCAAGAA 
TTCGATTTAG AAAACATCAA TGATGTGGTG GAAGTTAGAG ATGGGGGAGA ATTTGACTCC 
CTGCTCCTAG CTGTGTACAC AGGACCTGGT CCTGTGAAGG ACTTGTTCTC TACCACCAAT 
AGGATGACTG TGATTTTCAC CACAAACATG GAGACCAGAC GCAAGGGCTT CAAAGCAAAT 
TTCACTTCCG GCTATTACTT GGGGATTCCA GAGCCCTGCC AAGATGACGA ATTTCAGTGC 
AAAGATGGAA ACTGCATTCC TCTGGGGAAT CTCTGTGATA GCTACCCGCA CTGTAGAGAT 
GGCTCAGATG AAGCAAGCTG TGTGCGTTTT CTTAATGGCA CAAGAAGCAA CAATGGGTTG 
GTACAGTTCA ATATCCATAG CATATGGCAT ATAGCTTGTG CTGAGAACTG GACCACACAA 
ATTTCAAATG AAGTTTGTCA TCTTCTGGGA CTAGGGAGTG CAAACTCATC AATGCCAATA 
TCATCTACCG GAGGTGGACC ATTTGTGAGA GTAAACCAAG CCCCTAATGG AAGCTTGATA 
CTAACTCCTA GTCTACAATG CTCCCAAGAT TCCTTGATTC TATTGCAATG CAACCATAAA 
TCATGTGGAG AAAAAAAGGT GACTCAGAAA GTCAGCCCAA AGATCGTTGG TGGAAGTGAC 
GCCCAGGCAG GGGCCTGGCC CTGGGTTGTG GCTTTGTATC ACAGAGACAG GTCTACAGAC 
CGTCTGCTCT GCGGTGCATC TCTTGTCAGC AGTGACTGGC TGGTGTCAGC TGCACACTGT 
GTGTATAGAA GAAATCTAGA TCCAACCAGA TGGACAGCAG TCCTGGGCCT GCATATGCAA 
TCAAATCTGA CTTCTCCTCA AGTAGTAAGA CGGGTGGTTG ATCAAATTGT CATAAACCCT 
CATTATGACA GACGCAGAAA GGTCAACGAC ATCGCTATGA TGCACCTTGA GTTTAAAGTA 
AATTACACAG ATTATATACA GCCTATTTGT TTACCAGAAG AGAATCAAAT ATTTATTCCA 
GGAAGAACTT GTTCGATTGC TGGCTGGGGA TATGATAAAA TAAATGCAGG TTCTACTGTT 
GATGTGCTGA AAGAAGCTGA TGTTCCTCTT ATATCCAATG AAAAGTGCCA GCAACAGCTG 
CCAGAATACA ACATCACTGA AAGTATGATA TGTGCAGGAT ATGAAGAAGG AGGAATAGAT 
TCTTGTCAGG GTGATTCTGG AGGGCCATTA ATGTGCCAAG AAAACAACAG ATGGTTTCTT 
GTTGGTGTGA CATCATTTGG AGTCCAGTGT GCACTGCCTA ATCACCCTGG GGTATATGTC 
CGAGTCTCAC AGTTTATAGA ATGGATCCAT AGCTTTCTAC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition effect of enteropeptidase on RANKL-RANK signalling by cleavage of RANK.
FEBS letters587(18)2958-64(2013 Sep)
Zhao Y,Jin M,Ma J,Zhang S,Li W,Chen Y,Zhou Y,Tao H,Liu Y,Wang L,Han H,Niu G,Tao H,Liu C,Gao B

Gene expression profiling in a mouse model for African trypanosomiasis.
Genes and immunity7(8)667-79(2006 Dec)
Kierstein S,Noyes H,Naessens J,Nakamura Y,Pritchard C,Gibson J,Kemp S,Brass A

Structure of murine enterokinase (enteropeptidase) and expression in small intestine during development.
The American journal of physiology274(2 Pt 1)G342-9(1998 Feb)
Yuan X,Zheng X,Lu D,Rubin DC,Pung CY,Sadler JE

Two-dimensional gel analysis of zymogen-activating factors in the small intestine of the mouse.
The Journal of experimental zoology231(1)19-26(1984 Jul)
Isobe M,Ogita Z

Electrophoretic analysis of pancreatic proteases and zymogen-activating factors in the mouse.
The Journal of experimental zoology230(3)347-54(1984 Jun)
Isobe M,Ogita Z

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Tmprss15 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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