Prkcsh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkcsh
Entrez Gene ID	19089
Full Name	protein kinase C substrate 80K-H
Synonyms	80K-H,PKCSH
General protein information	Preferred Names protein kinase C substrate 80K-H Names glucosidase 2 subunit beta alpha glucosidase II, beta-subunit carbohydrate processing enzyme of the endoplasmic reticulum glucosidase II beta-subunit hepatocystin protein kinase C substrate 60.1 kDa protein heavy chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A3\|9 8.04 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001293650.1	NP_001280579.1	glucosidase 2 subunit beta isoform 1 precursor
NM_001293651.1	NP_001280580.1	glucosidase 2 subunit beta isoform 2 precursor
XM_006510096.2	XP_006510159.1	glucosidase 2 subunit beta isoform X1
NM_008925.2	NP_032951.1	glucosidase 2 subunit beta isoform 2 precursor
XM_006510095.3	XP_006510158.1	glucosidase 2 subunit beta isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkcsh	NP_032951.1
Drosophila melanogaster (fruit fly)	CG6453	NP_001246063.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004609	XP_315213.4
Xenopus tropicalis (tropical clawed frog)	prkcsh	NP_989356.1
Homo sapiens (human)	PRKCSH	NP_001001329.1
Pan troglodytes (chimpanzee)	PRKCSH	XP_003316136.2
Canis lupus familiaris (dog)	PRKCSH	XP_005632910.1
Caenorhabditis elegans (roundworm)	CELE_ZK1307.8	NP_496073.1
Danio rerio (zebrafish)	prkcsh	NP_957347.1
Bos taurus (cattle)	PRKCSH	NP_788835.1
Rattus norvegicus (Norway rat)	Prkcsh	NP_001100276.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of mammalian ER ?-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Caputo AT, Alonzi DS, Marti L, Reca IB, Kiappes JL, Struwe WB, Cross A, Basu S, Lowe ED, Darlot B, Santino A, Roversi P, Zitzmann N
Proceedings of the National Academy of Sciences of the United States of America113(32)E4630-8(2016 08)

Sec63 and Xbp1 regulate IRE1? activity and polycystic disease severity.
Fedeles SV, So JS, Shrikhande A, Lee SH, Gallagher AR, Barkauskas CE, Somlo S, Lee AH
The Journal of clinical investigation125(5)1955-67(2015 May)

Hepatocystin contributes to interferon-mediated antiviral response to hepatitis B virus by regulating hepatocyte nuclear factor 4?.
Shin GC, Ahn SH, Choi HS, Kim J, Park ES, Kim DH, Kim KH
Biochimica et biophysica acta1842(9)1648-57(2014 Sep)

TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis.
Yaguchi H, Okumura F, Takahashi H, Kano T, Kameda H, Uchigashima M, Tanaka S, Watanabe M, Sasaki H, Hatakeyama S
The Journal of biological chemistry287(15)12050-9(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TRIM67 regulates Ras signaling via degradation of 80K-H, leading to neural differentiation including neuritogenesis.
Title: TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis.

Data demonstrate the presence of a binding site on the 3'-untranslated region of NR1 mRNA for AnxA2 and show the regulation of NR1 mRNA by AnxA2, GIIbeta and a third NR1 mRNA-binding protein, which is yet to be identified.
Title: A cis-acting region in the N-methyl-d-aspartate R1 3'-untranslated region interacts with the novel RNA-binding proteins beta subunit of alpha glucosidase II and annexin A2--effect of chronic ethanol exposure in vivo.

80K-H is a novel regulator of IP3R1 activity, and it may contribute to neuronal functions.
Title: 80K-H interacts with inositol 1,4,5-trisphosphate (IP3) receptors and regulates IP3-induced calcium release activity.

80K-H is a Ca(2+) sensor controlling TRPV5 channel activity
Title: 80K-H as a new Ca2+ sensor regulating the activity of the epithelial Ca2+ channel transient receptor potential cation channel V5 (TRPV5).

The following Prkcsh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkcsh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23442	NM_008925.2 Latest version!	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu23442	NM_001293651.1 Latest version!	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu23444	NM_001293650.1 Latest version!	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu23444	XM_006510095.3 Latest version!	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu23444	XM_006510096.2 Latest version!	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkcsh ( NM_001293651.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001280580.1 Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu23442
Clone ID Related Accession (Same CDS sequence)	NM_001293651.1 , NM_008925.2
Accession Version	NM_001293651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glucosidase 2 subunit beta isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY295975.1, CD807078.1, AK137661.1 and AC163623.5. On Jun 12, 2014 this sequence version replaced XM_006510097.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Variants 2 and 3 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG GCTCCGCCCC CACTGCAGCC CCCACAGCCT 
CCCAGCCCCA CAGAGGATGA GAAGATGCCG CCCTATGATG AGGAGACCCA GGCCATCATC 
GATGCTGCAC AGGAGGCCCG GAGTAAGTTT GAGGAAGTCG AACGGTCCTT GAAAGAGATG 
GAAGAGTCCA TCAGGAGTTT GGAACAAGAG ATCTCCTTTG ATTTCGGTCC CTCTGGAGAG 
TTTGCATATC TCTACAGCCA ATGCTACGAG CTCACCACCA ATGAGTACGT CTACCGGCTT 
TGCCCCTTCA AACTGGTCTC CCAGAAACCC AAACATGGGG GCTCCCCGAC CAGCCTGGGC 
ACATGGGGCT CCTGGGCTGG CCCTGATCAT GACAAGTTCA GTGCGATGAA GTACGAGCAG 
GGCACGGGCT GTTGGCAGGG CCCCAACCGA TCCACCACAG TGCGCCTGCT GTGTGGCAAA 
GAGACTGTGG TGACCAGCAC CACGGAGCCC AGTCGCTGTG AGTACCTCAT GGAGCTGATG 
ACACCAGCAG CCTGCCCAGA GCCGCCACCA GAAGCACCCA GTGATGGGGA CCATGACGAG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280580.1
CDS	166..1731
Translation	MLLLLLLLLPLCWAVEVKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEETEPKEEKPPVLPPTEEEEEEEEEPEEEEEEEEEEEEAPPPLQPPQPPSPTEDEKMPPYDEETQAIIDAAQEARSKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL

Target ORF information:

RefSeq Version	NM_001293651.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293651.1

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG GCTCCGCCCC CACTGCAGCC CCCACAGCCT 
CCCAGCCCCA CAGAGGATGA GAAGATGCCG CCCTATGATG AGGAGACCCA GGCCATCATC 
GATGCTGCAC AGGAGGCCCG GAGTAAGTTT GAGGAAGTCG AACGGTCCTT GAAAGAGATG 
GAAGAGTCCA TCAGGAGTTT GGAACAAGAG ATCTCCTTTG ATTTCGGTCC CTCTGGAGAG 
TTTGCATATC TCTACAGCCA ATGCTACGAG CTCACCACCA ATGAGTACGT CTACCGGCTT 
TGCCCCTTCA AACTGGTCTC CCAGAAACCC AAACATGGGG GCTCCCCGAC CAGCCTGGGC 
ACATGGGGCT CCTGGGCTGG CCCTGATCAT GACAAGTTCA GTGCGATGAA GTACGAGCAG 
GGCACGGGCT GTTGGCAGGG CCCCAACCGA TCCACCACAG TGCGCCTGCT GTGTGGCAAA 
GAGACTGTGG TGACCAGCAC CACGGAGCCC AGTCGCTGTG AGTACCTCAT GGAGCTGATG 
ACACCAGCAG CCTGCCCAGA GCCGCCACCA GAAGCACCCA GTGATGGGGA CCATGACGAG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcsh ( NM_008925.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032951.1 Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23442
Clone ID Related Accession (Same CDS sequence)	NM_001293651.1 , NM_008925.2
Accession Version	NM_008925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glucosidase 2 subunit beta isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY295975.1, AK137661.1 and AC163623.5. On Jun 12, 2014 this sequence version replaced NM_008925.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Variants 2 and 3 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159705.1, AK137661.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG GCTCCGCCCC CACTGCAGCC CCCACAGCCT 
CCCAGCCCCA CAGAGGATGA GAAGATGCCG CCCTATGATG AGGAGACCCA GGCCATCATC 
GATGCTGCAC AGGAGGCCCG GAGTAAGTTT GAGGAAGTCG AACGGTCCTT GAAAGAGATG 
GAAGAGTCCA TCAGGAGTTT GGAACAAGAG ATCTCCTTTG ATTTCGGTCC CTCTGGAGAG 
TTTGCATATC TCTACAGCCA ATGCTACGAG CTCACCACCA ATGAGTACGT CTACCGGCTT 
TGCCCCTTCA AACTGGTCTC CCAGAAACCC AAACATGGGG GCTCCCCGAC CAGCCTGGGC 
ACATGGGGCT CCTGGGCTGG CCCTGATCAT GACAAGTTCA GTGCGATGAA GTACGAGCAG 
GGCACGGGCT GTTGGCAGGG CCCCAACCGA TCCACCACAG TGCGCCTGCT GTGTGGCAAA 
GAGACTGTGG TGACCAGCAC CACGGAGCCC AGTCGCTGTG AGTACCTCAT GGAGCTGATG 
ACACCAGCAG CCTGCCCAGA GCCGCCACCA GAAGCACCCA GTGATGGGGA CCATGACGAG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032951.1
CDS	162..1727
Translation	MLLLLLLLLPLCWAVEVKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEETEPKEEKPPVLPPTEEEEEEEEEPEEEEEEEEEEEEAPPPLQPPQPPSPTEDEKMPPYDEETQAIIDAAQEARSKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL

Target ORF information:

RefSeq Version	NM_008925.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008925.2

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG GCTCCGCCCC CACTGCAGCC CCCACAGCCT 
CCCAGCCCCA CAGAGGATGA GAAGATGCCG CCCTATGATG AGGAGACCCA GGCCATCATC 
GATGCTGCAC AGGAGGCCCG GAGTAAGTTT GAGGAAGTCG AACGGTCCTT GAAAGAGATG 
GAAGAGTCCA TCAGGAGTTT GGAACAAGAG ATCTCCTTTG ATTTCGGTCC CTCTGGAGAG 
TTTGCATATC TCTACAGCCA ATGCTACGAG CTCACCACCA ATGAGTACGT CTACCGGCTT 
TGCCCCTTCA AACTGGTCTC CCAGAAACCC AAACATGGGG GCTCCCCGAC CAGCCTGGGC 
ACATGGGGCT CCTGGGCTGG CCCTGATCAT GACAAGTTCA GTGCGATGAA GTACGAGCAG 
GGCACGGGCT GTTGGCAGGG CCCCAACCGA TCCACCACAG TGCGCCTGCT GTGTGGCAAA 
GAGACTGTGG TGACCAGCAC CACGGAGCCC AGTCGCTGTG AGTACCTCAT GGAGCTGATG 
ACACCAGCAG CCTGCCCAGA GCCGCCACCA GAAGCACCCA GTGATGGGGA CCATGACGAG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcsh ( NM_001293650.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001280579.1 Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23444
Clone ID Related Accession (Same CDS sequence)	NM_001293650.1 , XM_006510096.2 , XM_006510095.3
Accession Version	NM_001293650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glucosidase 2 subunit beta isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY295975.1, AK137661.1, CX207653.1 and AC163623.5. On Jun 12, 2014 this sequence version replaced XM_006510094.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGTG CAGGGGGAGC AGCCCAAGGA GGCTCCGCCC 
CCACTGCAGC CCCCACAGCC TCCCAGCCCC ACAGAGGATG AGAAGATGCC GCCCTATGAT 
GAGGAGACCC AGGCCATCAT CGATGCTGCA CAGGAGGCCC GGAGTAAGTT TGAGGAAGTC 
GAACGGTCCT TGAAAGAGAT GGAAGAGTCC ATCAGGAGTT TGGAACAAGA GATCTCCTTT 
GATTTCGGTC CCTCTGGAGA GTTTGCATAT CTCTACAGCC AATGCTACGA GCTCACCACC 
AATGAGTACG TCTACCGGCT TTGCCCCTTC AAACTGGTCT CCCAGAAACC CAAACATGGG 
GGCTCCCCGA CCAGCCTGGG CACATGGGGC TCCTGGGCTG GCCCTGATCA TGACAAGTTC 
AGTGCGATGA AGTACGAGCA GGGCACGGGC TGTTGGCAGG GCCCCAACCG ATCCACCACA 
GTGCGCCTGC TGTGTGGCAA AGAGACTGTG GTGACCAGCA CCACGGAGCC CAGTCGCTGT 
GAGTACCTCA TGGAGCTGAT GACACCAGCA GCCTGCCCAG AGCCGCCACC AGAAGCACCC 
AGTGATGGGG ACCATGACGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280579.1
CDS	162..1748
Translation	MLLLLLLLLPLCWAVEVKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEETEPKEEKPPVLPPTEEEEEEEEEPEEEEEEEEEEEVQGEQPKEAPPPLQPPQPPSPTEDEKMPPYDEETQAIIDAAQEARSKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL

Target ORF information:

RefSeq Version	NM_001293650.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293650.1

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGTG CAGGGGGAGC AGCCCAAGGA GGCTCCGCCC 
CCACTGCAGC CCCCACAGCC TCCCAGCCCC ACAGAGGATG AGAAGATGCC GCCCTATGAT 
GAGGAGACCC AGGCCATCAT CGATGCTGCA CAGGAGGCCC GGAGTAAGTT TGAGGAAGTC 
GAACGGTCCT TGAAAGAGAT GGAAGAGTCC ATCAGGAGTT TGGAACAAGA GATCTCCTTT 
GATTTCGGTC CCTCTGGAGA GTTTGCATAT CTCTACAGCC AATGCTACGA GCTCACCACC 
AATGAGTACG TCTACCGGCT TTGCCCCTTC AAACTGGTCT CCCAGAAACC CAAACATGGG 
GGCTCCCCGA CCAGCCTGGG CACATGGGGC TCCTGGGCTG GCCCTGATCA TGACAAGTTC 
AGTGCGATGA AGTACGAGCA GGGCACGGGC TGTTGGCAGG GCCCCAACCG ATCCACCACA 
GTGCGCCTGC TGTGTGGCAA AGAGACTGTG GTGACCAGCA CCACGGAGCC CAGTCGCTGT 
GAGTACCTCA TGGAGCTGAT GACACCAGCA GCCTGCCCAG AGCCGCCACC AGAAGCACCC 
AGTGATGGGG ACCATGACGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcsh ( XM_006510096.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510159.1 Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant X2, mRNA.

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CloneID	OMu23444
Clone ID Related Accession (Same CDS sequence)	NM_001293650.1 , XM_006510096.2 , XM_006510095.3
Accession Version	XM_006510096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glucosidase 2 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006510096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGTG CAGGGGGAGC AGCCCAAGGA GGCTCCGCCC 
CCACTGCAGC CCCCACAGCC TCCCAGCCCC ACAGAGGATG AGAAGATGCC GCCCTATGAT 
GAGGAGACCC AGGCCATCAT CGATGCTGCA CAGGAGGCCC GGAGTAAGTT TGAGGAAGTC 
GAACGGTCCT TGAAAGAGAT GGAAGAGTCC ATCAGGAGTT TGGAACAAGA GATCTCCTTT 
GATTTCGGTC CCTCTGGAGA GTTTGCATAT CTCTACAGCC AATGCTACGA GCTCACCACC 
AATGAGTACG TCTACCGGCT TTGCCCCTTC AAACTGGTCT CCCAGAAACC CAAACATGGG 
GGCTCCCCGA CCAGCCTGGG CACATGGGGC TCCTGGGCTG GCCCTGATCA TGACAAGTTC 
AGTGCGATGA AGTACGAGCA GGGCACGGGC TGTTGGCAGG GCCCCAACCG ATCCACCACA 
GTGCGCCTGC TGTGTGGCAA AGAGACTGTG GTGACCAGCA CCACGGAGCC CAGTCGCTGT 
GAGTACCTCA TGGAGCTGAT GACACCAGCA GCCTGCCCAG AGCCGCCACC AGAAGCACCC 
AGTGATGGGG ACCATGACGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510159.1
CDS	80..1666
Translation	MLLLLLLLLPLCWAVEVKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEETEPKEEKPPVLPPTEEEEEEEEEPEEEEEEEEEEEVQGEQPKEAPPPLQPPQPPSPTEDEKMPPYDEETQAIIDAAQEARSKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL

Target ORF information:

RefSeq Version	XM_006510096.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510096.2

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGTG CAGGGGGAGC AGCCCAAGGA GGCTCCGCCC 
CCACTGCAGC CCCCACAGCC TCCCAGCCCC ACAGAGGATG AGAAGATGCC GCCCTATGAT 
GAGGAGACCC AGGCCATCAT CGATGCTGCA CAGGAGGCCC GGAGTAAGTT TGAGGAAGTC 
GAACGGTCCT TGAAAGAGAT GGAAGAGTCC ATCAGGAGTT TGGAACAAGA GATCTCCTTT 
GATTTCGGTC CCTCTGGAGA GTTTGCATAT CTCTACAGCC AATGCTACGA GCTCACCACC 
AATGAGTACG TCTACCGGCT TTGCCCCTTC AAACTGGTCT CCCAGAAACC CAAACATGGG 
GGCTCCCCGA CCAGCCTGGG CACATGGGGC TCCTGGGCTG GCCCTGATCA TGACAAGTTC 
AGTGCGATGA AGTACGAGCA GGGCACGGGC TGTTGGCAGG GCCCCAACCG ATCCACCACA 
GTGCGCCTGC TGTGTGGCAA AGAGACTGTG GTGACCAGCA CCACGGAGCC CAGTCGCTGT 
GAGTACCTCA TGGAGCTGAT GACACCAGCA GCCTGCCCAG AGCCGCCACC AGAAGCACCC 
AGTGATGGGG ACCATGACGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcsh ( XM_006510095.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510158.1 Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23444
Clone ID Related Accession (Same CDS sequence)	NM_001293650.1 , XM_006510096.2 , XM_006510095.3
Accession Version	XM_006510095.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glucosidase 2 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGTG CAGGGGGAGC AGCCCAAGGA GGCTCCGCCC 
CCACTGCAGC CCCCACAGCC TCCCAGCCCC ACAGAGGATG AGAAGATGCC GCCCTATGAT 
GAGGAGACCC AGGCCATCAT CGATGCTGCA CAGGAGGCCC GGAGTAAGTT TGAGGAAGTC 
GAACGGTCCT TGAAAGAGAT GGAAGAGTCC ATCAGGAGTT TGGAACAAGA GATCTCCTTT 
GATTTCGGTC CCTCTGGAGA GTTTGCATAT CTCTACAGCC AATGCTACGA GCTCACCACC 
AATGAGTACG TCTACCGGCT TTGCCCCTTC AAACTGGTCT CCCAGAAACC CAAACATGGG 
GGCTCCCCGA CCAGCCTGGG CACATGGGGC TCCTGGGCTG GCCCTGATCA TGACAAGTTC 
AGTGCGATGA AGTACGAGCA GGGCACGGGC TGTTGGCAGG GCCCCAACCG ATCCACCACA 
GTGCGCCTGC TGTGTGGCAA AGAGACTGTG GTGACCAGCA CCACGGAGCC CAGTCGCTGT 
GAGTACCTCA TGGAGCTGAT GACACCAGCA GCCTGCCCAG AGCCGCCACC AGAAGCACCC 
AGTGATGGGG ACCATGACGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510158.1
CDS	160..1746
Translation	MLLLLLLLLPLCWAVEVKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYRSEVPPTDIPVPEETEPKEEKPPVLPPTEEEEEEEEEPEEEEEEEEEEEVQGEQPKEAPPPLQPPQPPSPTEDEKMPPYDEETQAIIDAAQEARSKFEEVERSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL

Target ORF information:

RefSeq Version	XM_006510095.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C substrate 80K-H (Prkcsh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510095.3

ORF Insert Sequence:

ATGCTGCTGC TGCTGCTACT ACTACTACCC CTCTGTTGGG CTGTAGAAGT TAAGAGACCC 
CGGGGCGTTT CCCTCAGCAA CCATCACTTC TATGAAGAAT CTAAACCTTT CACCTGTTTG 
GACGGCACAG CCACCATCCC ATTCGATCAG GTGAACGACG ACTACTGCGA CTGTAAGGAC 
GGTTCAGATG AGCCTGGCAC AGCTGCTTGT CCCAATGGCA GCTTTCACTG CACCAACACT 
GGGTACAAGC CCTTGTACAT CCTCTCCAGC CGGGTCAATG ATGGGGTATG TGACTGCTGT 
GATGGCACAG ACGAGTACAA CAGCGGCACG GTCTGCGAGA ACACCTGCAG AGAGAAGGGT 
CGCAAGGAAA AAGAGTCCCT GCAGCAGCTG GCGGAAGTCA CCCGTGAAGG GTTCCGCCTG 
AAGAAGATTC TCATTGAGGA GTGGAAGACA GCCCGGGAAG AAAAGCAGAG TAAGCTTCTT 
GAGCTTCAGG CTGGAAAGAA GTCTCTGGAA GACCAGGTAG AAACACTGCG GGCAGCGAAA 
GAAGAAGCAG AGAGGCCAGA GAAGGAGGCC AAGGACCAGC ACCGGAAGCT GTGGGAAGAG 
CAGCAAGCTG CTGCCAAGGC CCGGCGGGAA CAGGAGCGGG CAGCCAGTGC CTTCCAGGAA 
CTTGACGACA ACATGGATGG GATGGTCTCG CTGGCTGAGT TACAGACTCA CCCGGAGCTG 
GACACAGATG GAGATGGAGC GCTGTCTGAG GAGGAGGCCC AGGCCCTTCT CAGTGGAGAC 
ACACAGACTG ACACCACCTC CTTCTATGAC CGTGTCTGGG CTGCCATCAG GGACAAGTAC 
CGCTCTGAGG TCCCGCCCAC TGACATACCT GTTCCGGAGG AGACTGAGCC CAAAGAGGAA 
AAGCCACCAG TGTTGCCACC CACAGAGGAG GAGGAAGAGG AGGAGGAGGA GCCAGAAGAA 
GAGGAGGAGG AAGAGGAAGA GGAGGAGGTG CAGGGGGAGC AGCCCAAGGA GGCTCCGCCC 
CCACTGCAGC CCCCACAGCC TCCCAGCCCC ACAGAGGATG AGAAGATGCC GCCCTATGAT 
GAGGAGACCC AGGCCATCAT CGATGCTGCA CAGGAGGCCC GGAGTAAGTT TGAGGAAGTC 
GAACGGTCCT TGAAAGAGAT GGAAGAGTCC ATCAGGAGTT TGGAACAAGA GATCTCCTTT 
GATTTCGGTC CCTCTGGAGA GTTTGCATAT CTCTACAGCC AATGCTACGA GCTCACCACC 
AATGAGTACG TCTACCGGCT TTGCCCCTTC AAACTGGTCT CCCAGAAACC CAAACATGGG 
GGCTCCCCGA CCAGCCTGGG CACATGGGGC TCCTGGGCTG GCCCTGATCA TGACAAGTTC 
AGTGCGATGA AGTACGAGCA GGGCACGGGC TGTTGGCAGG GCCCCAACCG ATCCACCACA 
GTGCGCCTGC TGTGTGGCAA AGAGACTGTG GTGACCAGCA CCACGGAGCC CAGTCGCTGT 
GAGTACCTCA TGGAGCTGAT GACACCAGCA GCCTGCCCAG AGCCGCCACC AGAAGCACCC 
AGTGATGGGG ACCATGACGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of mammalian ER ?-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Proceedings of the National Academy of Sciences of the United States of America113(32)E4630-8(2016 08)
Caputo AT,Alonzi DS,Marti L,Reca IB,Kiappes JL,Struwe WB,Cross A,Basu S,Lowe ED,Darlot B,Santino A,Roversi P,Zitzmann N

Sec63 and Xbp1 regulate IRE1? activity and polycystic disease severity.
The Journal of clinical investigation125(5)1955-67(2015 May)
Fedeles SV,So JS,Shrikhande A,Lee SH,Gallagher AR,Barkauskas CE,Somlo S,Lee AH

Hepatocystin contributes to interferon-mediated antiviral response to hepatitis B virus by regulating hepatocyte nuclear factor 4?.
Biochimica et biophysica acta1842(9)1648-57(2014 Sep)
Shin GC,Ahn SH,Choi HS,Kim J,Park ES,Kim DH,Kim KH

TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis.
The Journal of biological chemistry287(15)12050-9(2012 Apr)
Yaguchi H,Okumura F,Takahashi H,Kano T,Kameda H,Uchigashima M,Tanaka S,Watanabe M,Sasaki H,Hatakeyama S

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Prkcsh cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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