Ctsa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctsa
Entrez Gene ID	19025
Full Name	cathepsin A
Synonyms	AU019505,PPCA,Ppgb
General protein information	Preferred Names cathepsin A Names lysosomal protective protein carboxypeptidase C carboxypeptidase L protective protein cathepsin A protective protein for beta-galactosidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H3\|2 85.27 cM
Summary	This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030248567.1	XP_030104427.1	lysosomal protective protein isoform X1
NM_001038492.2	NP_001033581.1	lysosomal protective protein isoform b preproprotein
NM_008906.4	NP_032932.2	lysosomal protective protein isoform a preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	CTSA	NP_000299.2
	CTSA	XP_001106032.1
Caenorhabditis elegans (roundworm)	F41C3.5	NP_494846.1
Kluyveromyces lactis	KLLA0A09977g	XP_451436.1
Danio rerio (zebrafish)	ctsa	NP_956844.1
Arabidopsis thaliana (thale cress)	scpl21	NP_189169.1
Gallus gallus (chicken)	CTSA	NP_001026662.1
Schizosaccharomyces pombe (fission yeast)	cpy1	NP_594425.1
Oryza sativa (rice)	Os04g0321700	NP_001052464.1
Oryza sativa (rice)	Os12g0257000	NP_001066510.1
Xenopus tropicalis (tropical clawed frog)	ctsa	NP_001039207.1
Bos taurus (cattle)	CTSA	NP_001030403.1
Mus musculus (house mouse)	Ctsa	NP_032932.2
Arabidopsis thaliana (thale cress)	scpl20	NP_193027.5
Pan troglodytes (chimpanzee)	CTSA	XP_001159669.2
Canis lupus familiaris (dog)	CTSA	NP_001103385.1
Rattus norvegicus (Norway rat)	Ctsa	NP_001011959.1
Caenorhabditis elegans (roundworm)	F13D12.6	NP_496507.1
Saccharomyces cerevisiae (baker's yeast)	PRC1	NP_014026.1
Eremothecium gossypii	AGOS_AGL328C	NP_986339.1
Magnaporthe oryzae (rice blast fungus)	MGG_05663	XP_003710561.1
Neurospora crassa	NCU00477	XP_956796.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice, double deficient in lysosomal serine carboxypeptidases Scpep1 and Cathepsin A develop the hyperproliferative vesicular corneal dystrophy and hypertrophic skin thickenings.
Pan X, Wang Y, L?bke T, Hinek A, Pshezhetsky AV
PloS one12(2)e0172854(2017)

Elastin-derived peptides potentiate atherosclerosis through the immune Neu1-PI3K? pathway.
Gayral S, Garnotel R, Castaing-Berthou A, Blaise S, Fougerat A, Berge E, Montheil A, Malet N, Wymann MP, Maurice P, Debelle L, Martiny L, Martinez LO, Pshezhetsky AV, Duca L, Laffargue M
Cardiovascular research102(1)118-27(2014 Apr)

Serine carboxypeptidase SCPEP1 and Cathepsin A play complementary roles in regulation of vasoconstriction via inactivation of endothelin-1.
Pan X, Grigoryeva L, Seyrantepe V, Peng J, Kollmann K, Tremblay J, Lavoie JL, Hinek A, L?bke T, Pshezhetsky AV
PLoS genetics10(2)e1004146(2014 Feb)

Lack of PPCA expression only partially coincides with lysosomal storage in galactosialidosis mice: indirect evidence for spatial requirement of the catalytic rather than the protective function of PPCA.
Rottier RJ, Hahn CN, Mann LW, del Pilar Martin M, Smeyne RJ, Suzuki K, d'Azzo A
Human molecular genetics7(11)1787-94(1998 Oct)

A point mutation in the neu-1 locus causes the neuraminidase defect in the SM/J mouse.
Rottier RJ, Bonten E, d'Azzo A
Human molecular genetics7(2)313-21(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in normal tissues the tandem of serine carboxypeptidases, Scpep1 and CathA likely constitutes an important part of the physiological mechanism responsible for the balanced elimination of heightened levels of ET-1 that otherwise would accumulate in tissues and consequently contribute to development of the hyper-proliferative corneal dystrophy and abnormal skin thickening
Title: Mice, double deficient in lysosomal serine carboxypeptidases Scpep1 and Cathepsin A develop the hyperproliferative vesicular corneal dystrophy and hypertrophic skin thickenings.

our results define the biological role of mammalian serine carboxypeptidase Scpep1 and suggest that Scpep1 and CathA together participate in the control of ET-1 regulation of vascular tone and hemodynamics
Title: Serine carboxypeptidase SCPEP1 and Cathepsin A play complementary roles in regulation of vasoconstriction via inactivation of endothelin-1.

Loss of the cathepsin A results in the lysosomal storage disease galactosialidosis. However, mice with a catalytically inactive cathepsin A enzyme show no signs of this disease.
Title: Inhibition of CatA: an emerging strategy for the treatment of heart failure.

Results describe the hydrodynamic properties of PPCA, NEU1, and a complex of the two proteins and identified multiple binding sites on both proteins.
Title: Heterodimerization of the sialidase NEU1 with the chaperone protective protein/cathepsin A prevents its premature oligomerization.

The N-terminal N-glycan of NEU1 is indispensable for its function, whereas the C-terminal N-glycan appears to be non-essential. The omission of the second N-glycan can be compensated for by upregulating the expression of PPCA
Title: Protective protein/cathepsin A rescues N-glycosylation defects in neuraminidase-1.

The following Ctsa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctsa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03059	NM_008906.4 Latest version!	Mus musculus cathepsin A (Ctsa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu00195	NM_001038492.2 Latest version!	Mus musculus cathepsin A (Ctsa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$279.30 $399.00
OMu03059	XM_030248567.1 Latest version!	Mus musculus cathepsin A (Ctsa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctsa ( NM_008906.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032932.2 Mus musculus cathepsin A (Ctsa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03059
Clone ID Related Accession (Same CDS sequence)	XM_030248567.1 , NM_008906.4
Accession Version	NM_008906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Mus musculus(house mouse)
Product	lysosomal protective protein isoform a preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591495.12, AK159150.1, AK019861.1 and AK032197.1. On Jan 20, 2013 this sequence version replaced NM_008906.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK032197.1, AK159150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTCCA GTCCAAAGGC GCCTCCTGGA GAGCAAGGAC GCAAGGAAGC AGAGATGCCC 
GGAACCGCGC TGTCTCCACT GCTCTTGTTG CTGCTCCTGT CCTGGGCGTC CCGGAACGAA 
GCAGCTCCGG ACCAGGATGA AATCGATTGT CTCCCCGGCC TGGCCAAGCA GCCCTCTTTC 
CGGCAATACT CCGGCTACCT CAGAGCATCG GACTCCAAGC ACTTCCACTA CTGGTTTGTG 
GAGTCGCAGA ACGACCCAAA GAACAGCCCC GTGGTGCTTT GGCTTAACGG GGGTCCCGGC 
TGCAGCTCGC TCGATGGGCT GCTTACAGAG CACGGCCCCT TTCTGATCCA GCCAGATGGT 
GTCACCCTGG AGTACAACCC CTATGCTTGG AACCTGATTG CCAACGTGCT GTATATCGAG 
TCCCCAGCTG GGGTGGGCTT CTCCTACTCG GATGACAAGA TGTACGTGAC CAATGACACA 
GAGGTGGCGG AGAACAATTA TGAAGCCCTT AAAGACTTCT TCCGCCTCTT TCCGGAATAC 
AAGGACAACA AACTTTTCCT GACAGGAGAG AGCTATGCTG GCATCTACAT CCCCACCTTG 
GCTGTACTGG TCATGCAGGA TCCTAGCATG AATCTTCAGG GGCTGGCTGT GGGCAATGGA 
CTTGCCTCCT ATGAGCAGAA CGACAACTCC CTGGTCTACT TTGCCTACTA CCATGGCCTT 
CTGGGGAACA GACTTTGGAC TTCACTGCAG ACCCACTGCT GCGCTCAGAA CAAGTGTAAC 
TTCTATGACA ACAAAGACCC AGAGTGTGTA AACAATCTCC TGGAAGTGTC TCGAATTGTG 
GGCAAATCTG GCCTCAACAT CTACAATCTC TATGCTCCGT GTGCTGGTGG GGTGCCCGGC 
AGACATAGAT ATGAGGACAC ACTTGTAGTC CAGGATTTTG GCAACATCTT CACTCGCCTG 
CCACTTAAGC GGAGATTTCC TGAGGCACTG ATGCGTTCTG GGGACAAGGT ACGCTTGGAT 
CCTCCCTGCA CCAACACCAC AGCCCCTTCC AACTACCTCA ACAACCCCTA TGTTCGGAAG 
GCTCTCCACA TCCCCGAGTC GCTGCCCCGC TGGGACATGT GCAACTTCTT GGTGAATTTA 
CAGTACCGCC GCCTCTACCA AAGCATGAAC TCCCAGTACC TGAAGCTGCT CAGTTCACAG 
AAATACCAGA TCCTGCTCTA CAACGGAGAT GTGGACATGG CCTGCAACTT CATGGGCGAT 
GAGTGGTTTG TGGATTCGCT CAACCAGAAG ATGGAGGTGC AGCGCCGGCC CTGGCTAGTG 
GACTACGGGG AGAGCGGAGA ACAGGTAGCT GGTTTCGTGA AGGAGTGTTC ACACATCACC 
TTCCTCACCA TCAAGGGTGC CGGACACATG GTCCCCACGG ACAAGCCTCG AGCTGCTTTT 
ACCATGTTCT CGAGGTTCCT GAACAAAGAG CCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032932.2
CDS	25..1503
Translation	MTSSPKAPPGEQGRKEAEMPGTALSPLLLLLLLSWASRNEAAPDQDEIDCLPGLAKQPSFRQYSGYLRASDSKHFHYWFVESQNDPKNSPVVLWLNGGPGCSSLDGLLTEHGPFLIQPDGVTLEYNPYAWNLIANVLYIESPAGVGFSYSDDKMYVTNDTEVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEVSRIVGKSGLNIYNLYAPCAGGVPGRHRYEDTLVVQDFGNIFTRLPLKRRFPEALMRSGDKVRLDPPCTNTTAPSNYLNNPYVRKALHIPESLPRWDMCNFLVNLQYRRLYQSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVDYGESGEQVAGFVKECSHITFLTIKGAGHMVPTDKPRAAFTMFSRFLNKEPY

Target ORF information:

RefSeq Version	NM_008906.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin A (Ctsa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008906.4

ORF Insert Sequence:

ATGACTTCCA GTCCAAAGGC GCCTCCTGGA GAGCAAGGAC GCAAGGAAGC AGAGATGCCC 
GGAACCGCGC TGTCTCCACT GCTCTTGTTG CTGCTCCTGT CCTGGGCGTC CCGGAACGAA 
GCAGCTCCGG ACCAGGATGA AATCGATTGT CTCCCCGGCC TGGCCAAGCA GCCCTCTTTC 
CGGCAATACT CCGGCTACCT CAGAGCATCG GACTCCAAGC ACTTCCACTA CTGGTTTGTG 
GAGTCGCAGA ACGACCCAAA GAACAGCCCC GTGGTGCTTT GGCTTAACGG GGGTCCCGGC 
TGCAGCTCGC TCGATGGGCT GCTTACAGAG CACGGCCCCT TTCTGATCCA GCCAGATGGT 
GTCACCCTGG AGTACAACCC CTATGCTTGG AACCTGATTG CCAACGTGCT GTATATCGAG 
TCCCCAGCTG GGGTGGGCTT CTCCTACTCG GATGACAAGA TGTACGTGAC CAATGACACA 
GAGGTGGCGG AGAACAATTA TGAAGCCCTT AAAGACTTCT TCCGCCTCTT TCCGGAATAC 
AAGGACAACA AACTTTTCCT GACAGGAGAG AGCTATGCTG GCATCTACAT CCCCACCTTG 
GCTGTACTGG TCATGCAGGA TCCTAGCATG AATCTTCAGG GGCTGGCTGT GGGCAATGGA 
CTTGCCTCCT ATGAGCAGAA CGACAACTCC CTGGTCTACT TTGCCTACTA CCATGGCCTT 
CTGGGGAACA GACTTTGGAC TTCACTGCAG ACCCACTGCT GCGCTCAGAA CAAGTGTAAC 
TTCTATGACA ACAAAGACCC AGAGTGTGTA AACAATCTCC TGGAAGTGTC TCGAATTGTG 
GGCAAATCTG GCCTCAACAT CTACAATCTC TATGCTCCGT GTGCTGGTGG GGTGCCCGGC 
AGACATAGAT ATGAGGACAC ACTTGTAGTC CAGGATTTTG GCAACATCTT CACTCGCCTG 
CCACTTAAGC GGAGATTTCC TGAGGCACTG ATGCGTTCTG GGGACAAGGT ACGCTTGGAT 
CCTCCCTGCA CCAACACCAC AGCCCCTTCC AACTACCTCA ACAACCCCTA TGTTCGGAAG 
GCTCTCCACA TCCCCGAGTC GCTGCCCCGC TGGGACATGT GCAACTTCTT GGTGAATTTA 
CAGTACCGCC GCCTCTACCA AAGCATGAAC TCCCAGTACC TGAAGCTGCT CAGTTCACAG 
AAATACCAGA TCCTGCTCTA CAACGGAGAT GTGGACATGG CCTGCAACTT CATGGGCGAT 
GAGTGGTTTG TGGATTCGCT CAACCAGAAG ATGGAGGTGC AGCGCCGGCC CTGGCTAGTG 
GACTACGGGG AGAGCGGAGA ACAGGTAGCT GGTTTCGTGA AGGAGTGTTC ACACATCACC 
TTCCTCACCA TCAAGGGTGC CGGACACATG GTCCCCACGG ACAAGCCTCG AGCTGCTTTT 
ACCATGTTCT CGAGGTTCCT GAACAAAGAG CCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctsa ( NM_001038492.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001033581.1 Mus musculus cathepsin A (Ctsa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00195
Clone ID Related Accession (Same CDS sequence)	NM_001038492.2
Accession Version	NM_001038492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	lysosomal protective protein isoform b preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY319945.1, AK019861.1, AK032197.1 and AL591495.12. On Jan 20, 2013 this sequence version replaced NM_001038492.1. Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at an alternate downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK019861.1, J05261.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCCGGAA CCGCGCTGTC TCCACTGCTC TTGTTGCTGC TCCTGTCCTG GGCGTCCCGG 
AACGAAGCAG CTCCGGACCA GGATGAAATC GATTGTCTCC CCGGCCTGGC CAAGCAGCCC 
TCTTTCCGGC AATACTCCGG CTACCTCAGA GCATCGGACT CCAAGCACTT CCACTACTGG 
TTTGTGGAGT CGCAGAACGA CCCAAAGAAC AGCCCCGTGG TGCTTTGGCT TAACGGGGGT 
CCCGGCTGCA GCTCGCTCGA TGGGCTGCTT ACAGAGCACG GCCCCTTTCT GATCCAGCCA 
GATGGTGTCA CCCTGGAGTA CAACCCCTAT GCTTGGAACC TGATTGCCAA CGTGCTGTAT 
ATCGAGTCCC CAGCTGGGGT GGGCTTCTCC TACTCGGATG ACAAGATGTA CGTGACCAAT 
GACACAGAGG TGGCGGAGAA CAATTATGAA GCCCTTAAAG ACTTCTTCCG CCTCTTTCCG 
GAATACAAGG ACAACAAACT TTTCCTGACA GGAGAGAGCT ATGCTGGCAT CTACATCCCC 
ACCTTGGCTG TACTGGTCAT GCAGGATCCT AGCATGAATC TTCAGGGGCT GGCTGTGGGC 
AATGGACTTG CCTCCTATGA GCAGAACGAC AACTCCCTGG TCTACTTTGC CTACTACCAT 
GGCCTTCTGG GGAACAGACT TTGGACTTCA CTGCAGACCC ACTGCTGCGC TCAGAACAAG 
TGTAACTTCT ATGACAACAA AGACCCAGAG TGTGTAAACA ATCTCCTGGA AGTGTCTCGA 
ATTGTGGGCA AATCTGGCCT CAACATCTAC AATCTCTATG CTCCGTGTGC TGGTGGGGTG 
CCCGGCAGAC ATAGATATGA GGACACACTT GTAGTCCAGG ATTTTGGCAA CATCTTCACT 
CGCCTGCCAC TTAAGCGGAG ATTTCCTGAG GCACTGATGC GTTCTGGGGA CAAGGTACGC 
TTGGATCCTC CCTGCACCAA CACCACAGCC CCTTCCAACT ACCTCAACAA CCCCTATGTT 
CGGAAGGCTC TCCACATCCC CGAGTCGCTG CCCCGCTGGG ACATGTGCAA CTTCTTGGTG 
AATTTACAGT ACCGCCGCCT CTACCAAAGC ATGAACTCCC AGTACCTGAA GCTGCTCAGT 
TCACAGAAAT ACCAGATCCT GCTCTACAAC GGAGATGTGG ACATGGCCTG CAACTTCATG 
GGCGATGAGT GGTTTGTGGA TTCGCTCAAC CAGAAGATGG AGGTGCAGCG CCGGCCCTGG 
CTAGTGGACT ACGGGGAGAG CGGAGAACAG GTAGCTGGTT TCGTGAAGGA GTGTTCACAC 
ATCACCTTCC TCACCATCAA GGGTGCCGGA CACATGGTCC CCACGGACAA GCCTCGAGCT 
GCTTTTACCA TGTTCTCGAG GTTCCTGAAC AAAGAGCCTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033581.1
CDS	326..1750
Translation	MPGTALSPLLLLLLLSWASRNEAAPDQDEIDCLPGLAKQPSFRQYSGYLRASDSKHFHYWFVESQNDPKNSPVVLWLNGGPGCSSLDGLLTEHGPFLIQPDGVTLEYNPYAWNLIANVLYIESPAGVGFSYSDDKMYVTNDTEVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEVSRIVGKSGLNIYNLYAPCAGGVPGRHRYEDTLVVQDFGNIFTRLPLKRRFPEALMRSGDKVRLDPPCTNTTAPSNYLNNPYVRKALHIPESLPRWDMCNFLVNLQYRRLYQSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVDYGESGEQVAGFVKECSHITFLTIKGAGHMVPTDKPRAAFTMFSRFLNKEPY

Target ORF information:

RefSeq Version	NM_001038492.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin A (Ctsa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038492.2

ORF Insert Sequence:

ATGCCCGGAA CCGCGCTGTC TCCACTGCTC TTGTTGCTGC TCCTGTCCTG GGCGTCCCGG 
AACGAAGCAG CTCCGGACCA GGATGAAATC GATTGTCTCC CCGGCCTGGC CAAGCAGCCC 
TCTTTCCGGC AATACTCCGG CTACCTCAGA GCATCGGACT CCAAGCACTT CCACTACTGG 
TTTGTGGAGT CGCAGAACGA CCCAAAGAAC AGCCCCGTGG TGCTTTGGCT TAACGGGGGT 
CCCGGCTGCA GCTCGCTCGA TGGGCTGCTT ACAGAGCACG GCCCCTTTCT GATCCAGCCA 
GATGGTGTCA CCCTGGAGTA CAACCCCTAT GCTTGGAACC TGATTGCCAA CGTGCTGTAT 
ATCGAGTCCC CAGCTGGGGT GGGCTTCTCC TACTCGGATG ACAAGATGTA CGTGACCAAT 
GACACAGAGG TGGCGGAGAA CAATTATGAA GCCCTTAAAG ACTTCTTCCG CCTCTTTCCG 
GAATACAAGG ACAACAAACT TTTCCTGACA GGAGAGAGCT ATGCTGGCAT CTACATCCCC 
ACCTTGGCTG TACTGGTCAT GCAGGATCCT AGCATGAATC TTCAGGGGCT GGCTGTGGGC 
AATGGACTTG CCTCCTATGA GCAGAACGAC AACTCCCTGG TCTACTTTGC CTACTACCAT 
GGCCTTCTGG GGAACAGACT TTGGACTTCA CTGCAGACCC ACTGCTGCGC TCAGAACAAG 
TGTAACTTCT ATGACAACAA AGACCCAGAG TGTGTAAACA ATCTCCTGGA AGTGTCTCGA 
ATTGTGGGCA AATCTGGCCT CAACATCTAC AATCTCTATG CTCCGTGTGC TGGTGGGGTG 
CCCGGCAGAC ATAGATATGA GGACACACTT GTAGTCCAGG ATTTTGGCAA CATCTTCACT 
CGCCTGCCAC TTAAGCGGAG ATTTCCTGAG GCACTGATGC GTTCTGGGGA CAAGGTACGC 
TTGGATCCTC CCTGCACCAA CACCACAGCC CCTTCCAACT ACCTCAACAA CCCCTATGTT 
CGGAAGGCTC TCCACATCCC CGAGTCGCTG CCCCGCTGGG ACATGTGCAA CTTCTTGGTG 
AATTTACAGT ACCGCCGCCT CTACCAAAGC ATGAACTCCC AGTACCTGAA GCTGCTCAGT 
TCACAGAAAT ACCAGATCCT GCTCTACAAC GGAGATGTGG ACATGGCCTG CAACTTCATG 
GGCGATGAGT GGTTTGTGGA TTCGCTCAAC CAGAAGATGG AGGTGCAGCG CCGGCCCTGG 
CTAGTGGACT ACGGGGAGAG CGGAGAACAG GTAGCTGGTT TCGTGAAGGA GTGTTCACAC 
ATCACCTTCC TCACCATCAA GGGTGCCGGA CACATGGTCC CCACGGACAA GCCTCGAGCT 
GCTTTTACCA TGTTCTCGAG GTTCCTGAAC AAAGAGCCTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctsa ( XM_030248567.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104427.1 Mus musculus cathepsin A (Ctsa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03059
Clone ID Related Accession (Same CDS sequence)	XM_030248567.1 , NM_008906.4
Accession Version	XM_030248567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal protective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA GTCCAAAGGC GCCTCCTGGA GAGCAAGGAC GCAAGGAAGC AGAGATGCCC 
GGAACCGCGC TGTCTCCACT GCTCTTGTTG CTGCTCCTGT CCTGGGCGTC CCGGAACGAA 
GCAGCTCCGG ACCAGGATGA AATCGATTGT CTCCCCGGCC TGGCCAAGCA GCCCTCTTTC 
CGGCAATACT CCGGCTACCT CAGAGCATCG GACTCCAAGC ACTTCCACTA CTGGTTTGTG 
GAGTCGCAGA ACGACCCAAA GAACAGCCCC GTGGTGCTTT GGCTTAACGG GGGTCCCGGC 
TGCAGCTCGC TCGATGGGCT GCTTACAGAG CACGGCCCCT TTCTGATCCA GCCAGATGGT 
GTCACCCTGG AGTACAACCC CTATGCTTGG AACCTGATTG CCAACGTGCT GTATATCGAG 
TCCCCAGCTG GGGTGGGCTT CTCCTACTCG GATGACAAGA TGTACGTGAC CAATGACACA 
GAGGTGGCGG AGAACAATTA TGAAGCCCTT AAAGACTTCT TCCGCCTCTT TCCGGAATAC 
AAGGACAACA AACTTTTCCT GACAGGAGAG AGCTATGCTG GCATCTACAT CCCCACCTTG 
GCTGTACTGG TCATGCAGGA TCCTAGCATG AATCTTCAGG GGCTGGCTGT GGGCAATGGA 
CTTGCCTCCT ATGAGCAGAA CGACAACTCC CTGGTCTACT TTGCCTACTA CCATGGCCTT 
CTGGGGAACA GACTTTGGAC TTCACTGCAG ACCCACTGCT GCGCTCAGAA CAAGTGTAAC 
TTCTATGACA ACAAAGACCC AGAGTGTGTA AACAATCTCC TGGAAGTGTC TCGAATTGTG 
GGCAAATCTG GCCTCAACAT CTACAATCTC TATGCTCCGT GTGCTGGTGG GGTGCCCGGC 
AGACATAGAT ATGAGGACAC ACTTGTAGTC CAGGATTTTG GCAACATCTT CACTCGCCTG 
CCACTTAAGC GGAGATTTCC TGAGGCACTG ATGCGTTCTG GGGACAAGGT ACGCTTGGAT 
CCTCCCTGCA CCAACACCAC AGCCCCTTCC AACTACCTCA ACAACCCCTA TGTTCGGAAG 
GCTCTCCACA TCCCCGAGTC GCTGCCCCGC TGGGACATGT GCAACTTCTT GGTGAATTTA 
CAGTACCGCC GCCTCTACCA AAGCATGAAC TCCCAGTACC TGAAGCTGCT CAGTTCACAG 
AAATACCAGA TCCTGCTCTA CAACGGAGAT GTGGACATGG CCTGCAACTT CATGGGCGAT 
GAGTGGTTTG TGGATTCGCT CAACCAGAAG ATGGAGGTGC AGCGCCGGCC CTGGCTAGTG 
GACTACGGGG AGAGCGGAGA ACAGGTAGCT GGTTTCGTGA AGGAGTGTTC ACACATCACC 
TTCCTCACCA TCAAGGGTGC CGGACACATG GTCCCCACGG ACAAGCCTCG AGCTGCTTTT 
ACCATGTTCT CGAGGTTCCT GAACAAAGAG CCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104427.1
CDS	367..1845
Translation	MTSSPKAPPGEQGRKEAEMPGTALSPLLLLLLLSWASRNEAAPDQDEIDCLPGLAKQPSFRQYSGYLRASDSKHFHYWFVESQNDPKNSPVVLWLNGGPGCSSLDGLLTEHGPFLIQPDGVTLEYNPYAWNLIANVLYIESPAGVGFSYSDDKMYVTNDTEVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEVSRIVGKSGLNIYNLYAPCAGGVPGRHRYEDTLVVQDFGNIFTRLPLKRRFPEALMRSGDKVRLDPPCTNTTAPSNYLNNPYVRKALHIPESLPRWDMCNFLVNLQYRRLYQSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVDYGESGEQVAGFVKECSHITFLTIKGAGHMVPTDKPRAAFTMFSRFLNKEPY

Target ORF information:

RefSeq Version	XM_030248567.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin A (Ctsa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248567.1

ORF Insert Sequence:

ATGACTTCCA GTCCAAAGGC GCCTCCTGGA GAGCAAGGAC GCAAGGAAGC AGAGATGCCC 
GGAACCGCGC TGTCTCCACT GCTCTTGTTG CTGCTCCTGT CCTGGGCGTC CCGGAACGAA 
GCAGCTCCGG ACCAGGATGA AATCGATTGT CTCCCCGGCC TGGCCAAGCA GCCCTCTTTC 
CGGCAATACT CCGGCTACCT CAGAGCATCG GACTCCAAGC ACTTCCACTA CTGGTTTGTG 
GAGTCGCAGA ACGACCCAAA GAACAGCCCC GTGGTGCTTT GGCTTAACGG GGGTCCCGGC 
TGCAGCTCGC TCGATGGGCT GCTTACAGAG CACGGCCCCT TTCTGATCCA GCCAGATGGT 
GTCACCCTGG AGTACAACCC CTATGCTTGG AACCTGATTG CCAACGTGCT GTATATCGAG 
TCCCCAGCTG GGGTGGGCTT CTCCTACTCG GATGACAAGA TGTACGTGAC CAATGACACA 
GAGGTGGCGG AGAACAATTA TGAAGCCCTT AAAGACTTCT TCCGCCTCTT TCCGGAATAC 
AAGGACAACA AACTTTTCCT GACAGGAGAG AGCTATGCTG GCATCTACAT CCCCACCTTG 
GCTGTACTGG TCATGCAGGA TCCTAGCATG AATCTTCAGG GGCTGGCTGT GGGCAATGGA 
CTTGCCTCCT ATGAGCAGAA CGACAACTCC CTGGTCTACT TTGCCTACTA CCATGGCCTT 
CTGGGGAACA GACTTTGGAC TTCACTGCAG ACCCACTGCT GCGCTCAGAA CAAGTGTAAC 
TTCTATGACA ACAAAGACCC AGAGTGTGTA AACAATCTCC TGGAAGTGTC TCGAATTGTG 
GGCAAATCTG GCCTCAACAT CTACAATCTC TATGCTCCGT GTGCTGGTGG GGTGCCCGGC 
AGACATAGAT ATGAGGACAC ACTTGTAGTC CAGGATTTTG GCAACATCTT CACTCGCCTG 
CCACTTAAGC GGAGATTTCC TGAGGCACTG ATGCGTTCTG GGGACAAGGT ACGCTTGGAT 
CCTCCCTGCA CCAACACCAC AGCCCCTTCC AACTACCTCA ACAACCCCTA TGTTCGGAAG 
GCTCTCCACA TCCCCGAGTC GCTGCCCCGC TGGGACATGT GCAACTTCTT GGTGAATTTA 
CAGTACCGCC GCCTCTACCA AAGCATGAAC TCCCAGTACC TGAAGCTGCT CAGTTCACAG 
AAATACCAGA TCCTGCTCTA CAACGGAGAT GTGGACATGG CCTGCAACTT CATGGGCGAT 
GAGTGGTTTG TGGATTCGCT CAACCAGAAG ATGGAGGTGC AGCGCCGGCC CTGGCTAGTG 
GACTACGGGG AGAGCGGAGA ACAGGTAGCT GGTTTCGTGA AGGAGTGTTC ACACATCACC 
TTCCTCACCA TCAAGGGTGC CGGACACATG GTCCCCACGG ACAAGCCTCG AGCTGCTTTT 
ACCATGTTCT CGAGGTTCCT GAACAAAGAG CCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice, double deficient in lysosomal serine carboxypeptidases Scpep1 and Cathepsin A develop the hyperproliferative vesicular corneal dystrophy and hypertrophic skin thickenings.
PloS one12(2)e0172854(2017)
Pan X,Wang Y,L?bke T,Hinek A,Pshezhetsky AV

Elastin-derived peptides potentiate atherosclerosis through the immune Neu1-PI3K? pathway.
Cardiovascular research102(1)118-27(2014 Apr)
Gayral S,Garnotel R,Castaing-Berthou A,Blaise S,Fougerat A,Berge E,Montheil A,Malet N,Wymann MP,Maurice P,Debelle L,Martiny L,Martinez LO,Pshezhetsky AV,Duca L,Laffargue M

Serine carboxypeptidase SCPEP1 and Cathepsin A play complementary roles in regulation of vasoconstriction via inactivation of endothelin-1.
PLoS genetics10(2)e1004146(2014 Feb)
Pan X,Grigoryeva L,Seyrantepe V,Peng J,Kollmann K,Tremblay J,Lavoie JL,Hinek A,L?bke T,Pshezhetsky AV

Lack of PPCA expression only partially coincides with lysosomal storage in galactosialidosis mice: indirect evidence for spatial requirement of the catalytic rather than the protective function of PPCA.
Human molecular genetics7(11)1787-94(1998 Oct)
Rottier RJ,Hahn CN,Mann LW,del Pilar Martin M,Smeyne RJ,Suzuki K,d'Azzo A

A point mutation in the neu-1 locus causes the neuraminidase defect in the SM/J mouse.
Human molecular genetics7(2)313-21(1998 Feb)
Rottier RJ,Bonten E,d'Azzo A

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Ctsa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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