Ppef2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppef2
Entrez Gene ID	19023
Full Name	protein phosphatase, EF hand calcium-binding domain 2
General protein information	Preferred Names protein phosphatase, EF hand calcium-binding domain 2 Names serine/threonine-protein phosphatase with EF-hands 2 PPEF-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534817.2	XP_006534880.1	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
NM_011148.3	NP_035278.1	serine/threonine-protein phosphatase with EF-hands 2
NM_011148.4	NP_035278.1	serine/threonine-protein phosphatase with EF-hands 2
XM_030254206.1	XP_030110066.1	serine/threonine-protein phosphatase with EF-hands 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppef2	NP_001100680.1
Gallus gallus (chicken)	PPEF2	XP_420595.3
Drosophila melanogaster (fruit fly)	rdgC	NP_788544.1
Pan troglodytes (chimpanzee)	PPEF2	XP_526584.2
	PPEF2	XP_001100042.1
Mus musculus (house mouse)	Ppef2	NP_035278.1
Homo sapiens (human)	PPEF2	NP_006230.2
Canis lupus familiaris (dog)	PPEF2	XP_544934.3
Xenopus tropicalis (tropical clawed frog)	ppef2	XP_002938615.1
Bos taurus (cattle)	PPEF2	NP_001192906.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011414	XP_317894.4

Gene Ontology
Bibliography

Related articles in PubMed

Normal light response, photoreceptor integrity, and rhodopsin dephosphorylation in mice lacking both protein phosphatases with EF hands (PPEF-1 and PPEF-2).
Ramulu P, Kennedy M, Xiong WH, Williams J, Cowan M, Blesh D, Yau KW, Hurley JB, Nathans J
Molecular and cellular biology21(24)8605-14(2001 Dec)

Genomic structure and comparative analysis of nine Fugu genes: conservation of synteny with human chromosome Xp22.2-p22.1.
Brunner B, Todt T, Lenzner S, Stout K, Schulz U, Ropers HH, Kalscheuer VM
Genome research9(5)437-48(1999 May)

Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C.
Sherman PM, Sun H, Macke JP, Williams J, Smallwood PM, Nathans J
Proceedings of the National Academy of Sciences of the United States of America94(21)11639-44(1997 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07552	NM_011148.4 Latest version!	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu07552	XM_006534817.2 Latest version!	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu88767	XM_030254206.1 Latest version!	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu07552	NM_011148.3	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppef2 ( NM_011148.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035278.1 Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), mRNA.

Price & Availability↑

CloneID	OMu07552
Clone ID Related Accession (Same CDS sequence)	XM_006534817.2 , NM_011148.3 , NM_011148.4
Accession Version	NM_011148.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-29
Organism	Mus musculus(House mouse)
Product	serine/threonine-protein phosphatase with EF-hands 2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF023458.1. On Oct 21, 2007 this sequence version replaced NM_011148.2. ##Evidence-Data-START## Transcript exon combination :: AF023458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035278.1
CDS	1..2274
Translation	MGSSSSTQHHFAFQNAEKAFKAAALIQRWYRRYMARLEMRRRCTWNIFQSIEYAGQQDQVKLHEFFSYLVDHFTPSSHHERDFLNRMFTEERFAQDVETEEGGDFESIEVPDSYTGPRLSFPLLPDHATALVEAFRLRQQLHARYVLNLLYETRKHLAQLPNINRVSTCYSEEVTVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEVLMVLFAFMLVYPKEFHLNRGNHEDHLVNLRYGFTKEVMHKYKIHGKKILRTLQDVFCWLPLATLVDEKVLVLHGGVSDKTDLELLAKLDRHKIVSTMRCKTRKESENREEQKRKDNQTSSGQKPTPWFLPQSRSLPSSPFHLGSGFKAYKAGRSCSIPCGSPNSKELSRRGQVRRSVDLELEQCRQQAGFLGIREKGESLPLAPDADCVADGGGVLEPTPEEWKQVVDILWSDPAAQEGCKANAVRGGGCYFGPDVTERLMEKYKLQLLIRSHECKPEGYEFCHNRKVLTIFSASNYYEVGSNRGAYVKLGPALTPHIVQYQANKATHRLTMRQRISRVEESALRALRQKLFAHSSDLLVEFRKRDPDESGVITLSDWATAVESVLHLGLPWRMLRPQLVNSSADNVLEYRSWLDSLAKEQLSRENIQSSLLEKLYRNRSNLETIFRIIDSDHSGFISLDEFRQTWKLFSSHMSIDITDDGICDLARSIDFNKDGHIDINEFLEAFRLVEQSCLEGHASACLQSTDTAESGHSSPGPC

Target ORF information:

RefSeq Version	NM_011148.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011148.3

ORF Insert Sequence:

ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppef2 ( NM_011148.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035278.1 Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), mRNA.

Price & Availability↑

CloneID	OMu07552
Clone ID Related Accession (Same CDS sequence)	XM_006534817.2 , NM_011148.3 , NM_011148.4
Accession Version	NM_011148.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase with EF-hands 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125542.4. On or before Oct 11, 2017 this sequence version replaced XM_006534817.2, NM_011148.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC027049.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035278.1
CDS	310..2583
Translation	MGSSSSTQHHFAFQNAEKAFKAAALIQRWYRRYMARLEMRRRCTWNIFQSIEYAGQQDQVKLHEFFSYLVDHFTPSSHHERDFLNRMFTEERFAQDVETEEGGDFESIEVPDSYTGPRLSFPLLPDHATALVEAFRLRQQLHARYVLNLLYETRKHLAQLPNINRVSTCYSEEVTVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEVLMVLFAFMLVYPKEFHLNRGNHEDHLVNLRYGFTKEVMHKYKIHGKKILRTLQDVFCWLPLATLVDEKVLVLHGGVSDKTDLELLAKLDRHKIVSTMRCKTRKESENREEQKRKDNQTSSGQKPTPWFLPQSRSLPSSPFHLGSGFKAYKAGRSCSIPCGSPNSKELSRRGQVRRSVDLELEQCRQQAGFLGIREKGESLPLAPDADCVADGGGVLEPTPEEWKQVVDILWSDPAAQEGCKANAVRGGGCYFGPDVTERLMEKYKLQLLIRSHECKPEGYEFCHNRKVLTIFSASNYYEVGSNRGAYVKLGPALTPHIVQYQANKATHRLTMRQRISRVEESALRALRQKLFAHSSDLLVEFRKRDPDESGVITLSDWATAVESVLHLGLPWRMLRPQLVNSSADNVLEYRSWLDSLAKEQLSRENIQSSLLEKLYRNRSNLETIFRIIDSDHSGFISLDEFRQTWKLFSSHMSIDITDDGICDLARSIDFNKDGHIDINEFLEAFRLVEQSCLEGHASACLQSTDTAESGHSSPGPC

Target ORF information:

RefSeq Version	NM_011148.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011148.4

ORF Insert Sequence:

ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppef2 ( XM_006534817.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534880.1 Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu07552
Clone ID Related Accession (Same CDS sequence)	XM_006534817.2 , NM_011148.3 , NM_011148.4
Accession Version	XM_006534817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006534817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534880.1
CDS	932..3205
Translation	MGSSSSTQHHFAFQNAEKAFKAAALIQRWYRRYMARLEMRRRCTWNIFQSIEYAGQQDQVKLHEFFSYLVDHFTPSSHHERDFLNRMFTEERFAQDVETEEGGDFESIEVPDSYTGPRLSFPLLPDHATALVEAFRLRQQLHARYVLNLLYETRKHLAQLPNINRVSTCYSEEVTVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEVLMVLFAFMLVYPKEFHLNRGNHEDHLVNLRYGFTKEVMHKYKIHGKKILRTLQDVFCWLPLATLVDEKVLVLHGGVSDKTDLELLAKLDRHKIVSTMRCKTRKESENREEQKRKDNQTSSGQKPTPWFLPQSRSLPSSPFHLGSGFKAYKAGRSCSIPCGSPNSKELSRRGQVRRSVDLELEQCRQQAGFLGIREKGESLPLAPDADCVADGGGVLEPTPEEWKQVVDILWSDPAAQEGCKANAVRGGGCYFGPDVTERLMEKYKLQLLIRSHECKPEGYEFCHNRKVLTIFSASNYYEVGSNRGAYVKLGPALTPHIVQYQANKATHRLTMRQRISRVEESALRALRQKLFAHSSDLLVEFRKRDPDESGVITLSDWATAVESVLHLGLPWRMLRPQLVNSSADNVLEYRSWLDSLAKEQLSRENIQSSLLEKLYRNRSNLETIFRIIDSDHSGFISLDEFRQTWKLFSSHMSIDITDDGICDLARSIDFNKDGHIDINEFLEAFRLVEQSCLEGHASACLQSTDTAESGHSSPGPC

Target ORF information:

RefSeq Version	XM_006534817.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534817.2

ORF Insert Sequence:

ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppef2 ( XM_030254206.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110066.1 Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu88767
Clone ID Related Accession (Same CDS sequence)	XM_030254206.1
Accession Version	XM_030254206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase with EF-hands 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGTG TGAACCTCCC GCTGACAACA CCCCTTCCTC TTGTCAGCAA ACCCCTCCGT 
TCCCTCGGAT CGGGATCCGC CACTGCCGTC CATGTCCTGC AGGAGCGCTG TCCCTCAGCC 
ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110066.1
CDS	993..3386
Translation	MLCVNLPLTTPLPLVSKPLRSLGSGSATAVHVLQERCPSAMGSSSSTQHHFAFQNAEKAFKAAALIQRWYRRYMARLEMRRRCTWNIFQSIEYAGQQDQVKLHEFFSYLVDHFTPSSHHERDFLNRMFTEERFAQDVETEEGGDFESIEVPDSYTGPRLSFPLLPDHATALVEAFRLRQQLHARYVLNLLYETRKHLAQLPNINRVSTCYSEEVTVCGDLHGQLDDLIFIFYKNGLPSPERAYVFNGDFVDRGKDSVEVLMVLFAFMLVYPKEFHLNRGNHEDHLVNLRYGFTKEVMHKYKIHGKKILRTLQDVFCWLPLATLVDEKVLVLHGGVSDKTDLELLAKLDRHKIVSTMRCKTRKESENREEQKRKDNQTSSGQKPTPWFLPQSRSLPSSPFHLGSGFKAYKAGRSCSIPCGSPNSKELSRRGQVRRSVDLELEQCRQQAGFLGIREKGESLPLAPDADCVADGGGVLEPTPEEWKQVVDILWSDPAAQEGCKANAVRGGGCYFGPDVTERLMEKYKLQLLIRSHECKPEGYEFCHNRKVLTIFSASNYYEVGSNRGAYVKLGPALTPHIVQYQANKATHRLTMRQRISRVEESALRALRQKLFAHSSDLLVEFRKRDPDESGVITLSDWATAVESVLHLGLPWRMLRPQLVNSSADNVLEYRSWLDSLAKEQLSRENIQSSLLEKLYRNRSNLETIFRIIDSDHSGFISLDEFRQTWKLFSSHMSIDITDDGICDLARSIDFNKDGHIDINEFLEAFRLVEQSCLEGHASACLQSTDTAESGHSSPGPC

Target ORF information:

RefSeq Version	XM_030254206.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase, EF hand calcium-binding domain 2 (Ppef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254206.1

ORF Insert Sequence:

ATGCTGTGTG TGAACCTCCC GCTGACAACA CCCCTTCCTC TTGTCAGCAA ACCCCTCCGT 
TCCCTCGGAT CGGGATCCGC CACTGCCGTC CATGTCCTGC AGGAGCGCTG TCCCTCAGCC 
ATGGGAAGCA GCTCATCCAC CCAGCACCAC TTTGCGTTTC AGAACGCTGA GAAAGCCTTC 
AAGGCAGCGG CCCTGATCCA GAGATGGTAC CGGCGCTACA TGGCCCGCCT AGAGATGAGG 
AGGCGATGTA CCTGGAACAT CTTCCAGTCT ATAGAGTATG CTGGACAGCA AGACCAGGTC 
AAGCTCCATG AATTCTTCAG CTACCTTGTG GACCATTTCA CTCCCAGCAG CCACCATGAG 
AGAGATTTCC TGAACCGCAT GTTCACCGAA GAGAGATTCG CCCAGGACGT GGAGACAGAG 
GAGGGTGGAG ATTTTGAATC CATAGAGGTG CCAGACAGCT ACACGGGGCC ACGCCTCTCC 
TTCCCCCTTC TTCCTGACCA TGCCACTGCC CTGGTGGAAG CTTTCAGGCT GCGACAACAG 
CTCCATGCTC GATACGTCTT GAATCTTCTG TACGAGACCA GAAAACACCT GGCCCAGCTG 
CCCAACATCA ACCGAGTCTC CACCTGCTAC AGCGAGGAGG TCACAGTGTG TGGAGATCTA 
CATGGCCAGT TGGATGATTT AATATTTATA TTTTATAAGA ACGGTCTACC ATCACCAGAG 
AGAGCATACG TGTTCAATGG TGACTTTGTG GATCGAGGCA AGGACTCTGT AGAGGTCCTG 
ATGGTTCTCT TTGCCTTCAT GTTGGTATAC CCCAAGGAGT TCCATCTTAA CAGAGGAAAC 
CATGAGGACC ATCTAGTGAA CCTACGATAT GGCTTCACCA AGGAAGTGAT GCATAAATAC 
AAGATACATG GGAAGAAAAT CCTAAGGACA CTTCAGGATG TCTTCTGCTG GCTTCCGTTG 
GCCACTCTGG TTGATGAGAA AGTCCTTGTT CTTCATGGTG GAGTCTCAGA CAAGACAGAC 
CTGGAACTTC TGGCTAAACT AGACAGGCAC AAGATTGTTT CTACCATGAG GTGCAAAACA 
AGAAAGGAAA GTGAGAATCG AGAGGAGCAG AAGAGAAAAG ATAACCAGAC AAGCTCTGGA 
CAGAAACCTA CTCCGTGGTT TCTTCCTCAA AGCCGCTCTC TGCCCTCTTC GCCTTTTCAC 
CTGGGCTCTG GCTTTAAGGC CTACAAAGCC GGCAGGTCCT GCAGCATCCC CTGTGGCTCC 
CCTAACAGCA AGGAGCTATC CCGGCGGGGG CAGGTGCGGC GCTCAGTGGA CCTGGAACTG 
GAACAGTGCC GGCAGCAAGC CGGCTTCCTG GGGATCAGAG AGAAGGGGGA GTCCTTGCCT 
TTGGCCCCAG ATGCTGACTG TGTTGCTGAT GGGGGAGGGG TGCTGGAACC CACCCCCGAG 
GAGTGGAAGC AGGTTGTAGA TATTCTGTGG AGTGATCCCG CGGCTCAGGA GGGCTGCAAG 
GCCAACGCTG TCCGAGGAGG AGGCTGTTAC TTTGGGCCTG ACGTGACAGA ACGGTTGATG 
GAAAAATACA AGCTGCAACT CCTGATCCGT TCGCATGAGT GCAAACCCGA GGGCTATGAG 
TTCTGTCACA ACCGCAAGGT GTTAACCATC TTTTCTGCCT CCAACTACTA TGAAGTTGGC 
AGCAACAGAG GAGCCTACGT CAAGCTGGGG CCAGCCCTGA CTCCACATAT CGTGCAGTAT 
CAAGCTAACA AGGCGACCCA CAGGCTAACC ATGAGGCAAA GGATCAGCAG GGTGGAGGAG 
TCGGCCCTCA GAGCCCTTCG ACAGAAATTA TTTGCTCATT CGTCGGACCT GCTTGTTGAG 
TTTAGGAAGC GCGACCCGGA TGAGAGCGGT GTCATCACCC TGAGTGATTG GGCGACTGCC 
GTGGAGTCTG TGCTGCACCT GGGACTGCCC TGGCGGATGC TGCGGCCACA ACTGGTGAAT 
AGTTCAGCGG ATAACGTGTT GGAATACAGG TCCTGGTTGG ACAGTTTGGC CAAAGAACAG 
CTGAGCCGAG AGAATATTCA GTCGAGTTTG CTGGAAAAGC TCTATCGAAA CCGATCCAAC 
TTGGAGACCA TTTTTAGGAT CATAGACAGC GATCATTCAG GATTCATCTC CCTGGATGAG 
TTCAGGCAGA CTTGGAAGCT CTTCAGCTCA CATATGAGCA TCGACATCAC AGATGACGGC 
ATCTGTGACC TCGCCAGAAG CATCGACTTC AACAAGGATG GCCACATCGA CATCAATGAG 
TTCCTGGAGG CCTTCCGCCT CGTGGAGCAG TCCTGCTTAG AGGGCCACGC CTCTGCTTGC 
CTGCAGTCCA CAGACACTGC TGAGAGTGGC CATAGCAGTC CAGGCCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Normal light response, photoreceptor integrity, and rhodopsin dephosphorylation in mice lacking both protein phosphatases with EF hands (PPEF-1 and PPEF-2).
Molecular and cellular biology21(24)8605-14(2001 Dec)
Ramulu P,Kennedy M,Xiong WH,Williams J,Cowan M,Blesh D,Yau KW,Hurley JB,Nathans J

Genomic structure and comparative analysis of nine Fugu genes: conservation of synteny with human chromosome Xp22.2-p22.1.
Genome research9(5)437-48(1999 May)
Brunner B,Todt T,Lenzner S,Stout K,Schulz U,Ropers HH,Kalscheuer VM

Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C.
Proceedings of the National Academy of Sciences of the United States of America94(21)11639-44(1997 Oct)
Sherman PM,Sun H,Macke JP,Williams J,Smallwood PM,Nathans J

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Ppef2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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