Pold1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pold1
Entrez Gene ID	18971
Full Name	polymerase (DNA directed), delta 1, catalytic subunit
Synonyms	125kDa
General protein information	Preferred Names polymerase (DNA directed), delta 1, catalytic subunit Names DNA polymerase delta catalytic subunit DNA polymerase delta 1, catalytic domain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B3\|7 28.83 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030242220.1	XP_030098080.1	DNA polymerase delta catalytic subunit isoform X1
NM_011131.3	NP_035261.3	DNA polymerase delta catalytic subunit

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLD1	NP_001243778.1
Saccharomyces cerevisiae (baker's yeast)	POL3	NP_010181.2
Xenopus tropicalis (tropical clawed frog)	pold1	NP_001039204.1
Rattus norvegicus (Norway rat)	Pold1	NP_067694.1
Danio rerio (zebrafish)	pold1	NP_001034899.1
Neurospora crassa	NCU01192	XP_961558.1
Bos taurus (cattle)	POLD1	NP_776852.1
Mus musculus (house mouse)	Pold1	NP_035261.3
Drosophila melanogaster (fruit fly)	DNApol-delta	NP_524099.2
Caenorhabditis elegans (roundworm)	F10C2.4	NP_506017.1
Arabidopsis thaliana (thale cress)	EMB2780	NP_201201.2
Oryza sativa (rice)	Os11g0186400	NP_001067405.1
	POLD1	XP_001116065.1
Canis lupus familiaris (dog)	POLD1	XP_851285.1
Schizosaccharomyces pombe (fission yeast)	cdc6	NP_596124.1
Magnaporthe oryzae (rice blast fungus)	MGG_08071	XP_003715024.1
Pan troglodytes (chimpanzee)	POLD1	XP_003316601.2
Kluyveromyces lactis	KLLA0E01607g	XP_454020.1
Eremothecium gossypii	AGOS_AFL189W	NP_985361.2

Gene Ontology
Bibliography

Related articles in PubMed

Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice.
Uchimura A, Higuchi M, Minakuchi Y, Ohno M, Toyoda A, Fujiyama A, Miura I, Wakana S, Nishino J, Yagi T
Genome research25(8)1125-34(2015 Aug)

RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication.
Vannier JB, Sandhu S, Petalcorin MI, Wu X, Nabi Z, Ding H, Boulton SJ
Science (New York, N.Y.)342(6155)239-42(2013 Oct)

Use of somatic mutations to quantify random contributions to mouse development.
Zhou W, Tan Y, Anderson DJ, Crist EM, Ruohola-Baker H, Salipante SJ, Horwitz MS
BMC genomics1439(2013 Jan)

Protein arginine methyltransferase 7 regulates cellular response to DNA damage by methylating promoter histones H2A and H4 of the polymerase ? catalytic subunit gene, POLD1.
Karkhanis V, Wang L, Tae S, Hu YJ, Imbalzano AN, Sif S
The Journal of biological chemistry287(35)29801-14(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reducing expression of individual PRMT7 target DNA repair genes showed that only the catalytic subunit of DNA polymerase, POLD1, was able to resensitize PRMT7 knock-down cells to DNA-damaging agents.
Title: Protein arginine methyltransferase 7 regulates cellular response to DNA damage by methylating promoter histones H2A and H4 of the polymerase ? catalytic subunit gene, POLD1.

Findings distinguish Pol epsilon and delta functions in vivo and reveal tissue-specific requirements for DNA replication fidelity.
Title: DNA polymerase epsilon and delta proofreading suppress discrete mutator and cancer phenotypes in mice.

DNA polymerase delta is essential for mammalian early embryogenesis, and the 3'-5' exonuclease activity of DNA polymerase delta is dispensable for normal development but necessary to suppress tumorigenesis
Title: DNA polymerase delta is required for early mammalian embryogenesis.

Heterozygous mutation at L604 in the polymerase active site of DNA polymerase delta reduces life span, increases genomic instability, and accelerates tumorigenesis in an allele-specific manner, novel findings that have implications for human cancer.
Title: Mutation at the polymerase active site of mouse DNA polymerase delta increases genomic instability and accelerates tumorigenesis.

A point mutation (D400A) in the proofreading domain of DNA polymerase delta, encoded by the Pold1 gene, inactivates the 3' --> 5' exonuclease of poldelta and causes a mutator and epithelial cancer phenotype in a recessive manner.
Title: High incidence of epithelial cancers in mice deficient for DNA polymerase delta proofreading.

The following Pold1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pold1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07525	XM_030242220.1 Latest version!	Mus musculus polymerase (DNA directed), delta 1, catalytic subunit (Pold1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu07525	NM_011131.3 Latest version!	Mus musculus polymerase (DNA directed), delta 1, catalytic subunit (Pold1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pold1 ( XM_030242220.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098080.1 Mus musculus polymerase (DNA directed), delta 1, catalytic subunit (Pold1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu07525
Clone ID Related Accession (Same CDS sequence)	XM_030242220.1 , NM_011131.3
Accession Version	XM_030242220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA polymerase delta catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTA AGCGGCGACA AGGACCAGGC CCTGGGGTGC CCCCAAAGCG GGCTCGAGGG 
CACCTCTGGG ATGAGGACGA GCCTTCGCCG TCGCAGTTTG AGGCGAACCT GGCACTGCTG 
GAGGAAATAG AGGCTGAGAA CCGGCTGCAG GAGGCAGAGG AGGAGCTGCA GCTGCCCCCA 
GAGGGCACCG TGGGTGGGCA GTTTTCCACT GCAGACATTG ACCCTCGGTG GCGGCGGCCC 
ACCCTACGTG CCCTGGACCC CAGCACGGAG CCCCTCATCT TCCAGCAGCT GGAGATTGAC 
CACTATGTGG GCTCAGCACC ACCCCTGCCA GAAGGGCCCC TGCCATCCCG GAACTCAGTG 
CCCATACTGA GGGCCTTTGG GGTCACCGAT GAAGGCTTCT CCGTCTGCTG CCACATACAG 
GGCTTTGCCC CCTACTTCTA CACCCCCGCG CCTCCTGGTT TTGGGGCCGA GCACCTGAGT 
GAGCTGCAGC AGGAGCTGAA CGCAGCCATC AGCCGGGACC AGCGCGGTGG GAAGGAGCTC 
TCAGGGCCGG CAGTGCTGGC AATAGAGCTA TGCTCCCGTG AGAGCATGTT TGGGTACCAC 
GGTCATGGCC CTTCTCCATT TCTCCGCATC ACCCTGGCAC TACCCCGCCT TATGGCACCA 
GCCCGCCGCC TTCTGGAACA GGGTGTCCGA GTGCCAGGCC TGGGCACCCC GAGCTTCGCA 
CCCTACGAAG CCAACGTGGA CTTTGAGATC CGGTTCATGG TGGATGCTGA CATTGTGGGA 
TGCAACTGGT TGGAGCTGCC AGCTGGAAAG TACGTTCGGA GGGCGGAGAA GAAGGCCACC 
CTGTGTCAGC TGGAGGTGGA CGTGCTGTGG TCAGATGTGA TCAGTCACCC ACCGGAGGGG 
CAGTGGCAGC GCATTGCACC CCTGCGTGTG CTTAGCTTCG ACATCGAGTG TGCTGGCCGA 
AAAGGCATCT TCCCTGAGCC TGAGCGTGAC CCCGTGATCC AGATCTGTTC TCTGGGGCTG 
CGCTGGGGGG AGCCGGAGCC ATTCTTGCGT CTGGCACTCA CGCTGCGGCC CTGTGCCCCC 
ATCCTGGGTG CCAAAGTGCA GAGCTATGAG CGGGAAGAAG ACCTGCTCCA GGCCTGGGCC 
GACTTCATCC TTGCCATGGA CCCTGACGTG ATCACCGGCT ACAACATTCA GAACTTTGAC 
CTCCCATACC TCATCTCTCG GGCACAGGCC CTAAAGGTGG ACCGCTTCCC TTTCCTGGGC 
CGCGTGACTG GTCTCCGCTC CAACATCCGT GACTCCTCCT TCCAATCAAG GCAGGTCGGC 
CGGCGGGACA GTAAGGTGAT CAGCATGGTG GGTCGCGTTC AGATGGATAT GCTGCAGGTG 
CTGCTTCGGG AACACAAGCT CCGCTCCTAC ACGCTCAACG CTGTGAGTTT CCACTTCCTG 
GGCGAGCAGA AGGAGGACGT TCAGCACAGC ATCATCACCG ACCTGCAGAA TGGGAACGAA 
CAGACGCGCC GCCGCCTGGC CGTGTACTGC CTGAAGGACG CCTTTCTGCC ACTCCGACTA 
CTAGAGCGCC TTATGGTGCT GGTGAATAAT GTGGAGATGG CGCGTGTCAC GGGTGTACCC 
CTTGGGTACC TGCTCACCCG GGGCCAGCAG GTCAAGGTCG TGTCTCAGCT GCTGCGCCAG 
GCCATGCGCC AGGGGCTGCT GATGCCTGTG GTGAAGACCG AGGGCAGTGA GGACTACACG 
GGAGCCACAG TCATTGAGCC CCTCAAAGGG TACTATGACG TCCCCATTGC CACCCTGGAC 
TTCTCCTCCT TGTACCCATC CATCATGATG GCCCATAATC TGTGCTACAC CACGCTGCTC 
CGACCTGGGG CTGCCCAGAA GCTGGGCCTT AAACCAGATG AGTTCATCAA GACACCCACT 
GGGGATGAGT TTGTGAAGTC ATCTGTACGG AAGGGCCTCC TGCCCCAGAT CCTGGAGAAT 
CTGCTGAGTG CCCGCAAGAG GGCCAAGGCT GAGCTGGCTC AGGAGACGGA CCCCCTGCGG 
CGACAGGTCT TGGACGGCCG GCAACTGGCA CTAAAAGTGA GTGCCAACTC CGTATATGGC 
TTCACTGGTG CCCAGGTGGG CAAGCTGCCA TGTTTGGAGA TCTCCCAGAG TGTCACTGGG 
TTCGGGCGGC AGATGATTGA GAAAACCAAG CAGCTTGTGG AGTCCAAGTA CACCGTGGAA 
AATGGCTACG ATGCCAACGC CAAGGTAGTC TACGGTGACA CGGACTCTGT GATGTGCCGG 
TTTGGCGTCT CCTCTGTGGC TGAAGCAATG TCTCTGGGGC GGGAGGCTGC AAACTGGGTA 
TCCAGTCACT TCCCATCACC CATCCGGCTG GAGTTCGAGA AGGTTTACTT CCCATACCTG 
CTCATCAGCA AGAAGCGCTA TGCTGGCCTG CTCTTCTCCT CCCGCTCTGA TGCCCATGAC 
AAAATGGACT GCAAGGGCCT GGAGGCTGTG CGCAGGGACA ACTGTCCCCT GGTGGCCAAC 
CTCGTTACAT CCTCTCTGCG CCGGATCCTC GTGGACCGGG ACCCTGATGG GGCAGTAGCC 
CATGCCAAGG ACGTCATCTC GGACCTGCTG TGCAACCGCA TAGACATCTC CCAGCTGGTC 
ATCACCAAAG AGTTGACCCG CGCAGCAGCA GACTATGCTG GCAAGCAGGC TCACGTGGAG 
CTGGCTGAGA GGATGAGGAA GCGCGACCCC GGCAGTGCGC CCAGCCTGGG TGACCGAGTC 
CCCTATGTGA TCATTGGTGC TGCTAAGGGT GTGGCCGCCT ACATGAAGTC GGAGGACCCC 
CTGTTTGTGC TGGAGCACAG CCTGCCCATC GACACTCAGT ACTACCTGGA GCAGCAGCTG 
GCCAAGCCGC TCTTGCGCAT CTTTGAGCCC ATCCTGGGTG AGGGCCGTGC AGAGTCTGTG 
CTGCTGCGCG GTGACCACAC ACGATGCAAG ACTGTGCTCA CCAGCAAGGT GGGCGGCCTC 
TTGGCCTTCA CCAAGCGCCG CAACTGTTGC ATTGGCTGCC GCTCCGTAAT CGACCATCAA 
GGAGCCGTGT GTAAGTTCTG TCAGCCACGG GAGTCGGAGC TCTATCAGAA GGAGGTGTCA 
CACCTGAATG CCTTGGAAGA ACGGTTCTCT CGCCTCTGGA CACAGTGTCA ACGCTGCCAG 
GGCAGCTTGC ATGAGGACGT CATCTGTACC AGCCGTGACT GTCCCATCTT CTACATGCGC 
AAGAAGGTGC GCAAGGACCT GGAAGACCAG GAACGGCTGC TGCAGCGCTT TGGACCGCCC 
GGCCCTGAGG CCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098080.1
CDS	1216..4533
Translation	MDCKRRQGPGPGVPPKRARGHLWDEDEPSPSQFEANLALLEEIEAENRLQEAEEELQLPPEGTVGGQFSTADIDPRWRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVIDHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFGPPGPEAW

Target ORF information:

RefSeq Version	XM_030242220.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), delta 1, catalytic subunit (Pold1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242220.1

ORF Insert Sequence:

ATGGATTGTA AGCGGCGACA AGGACCAGGC CCTGGGGTGC CCCCAAAGCG GGCTCGAGGG 
CACCTCTGGG ATGAGGACGA GCCTTCGCCG TCGCAGTTTG AGGCGAACCT GGCACTGCTG 
GAGGAAATAG AGGCTGAGAA CCGGCTGCAG GAGGCAGAGG AGGAGCTGCA GCTGCCCCCA 
GAGGGCACCG TGGGTGGGCA GTTTTCCACT GCAGACATTG ACCCTCGGTG GCGGCGGCCC 
ACCCTACGTG CCCTGGACCC CAGCACGGAG CCCCTCATCT TCCAGCAGCT GGAGATTGAC 
CACTATGTGG GCTCAGCACC ACCCCTGCCA GAAGGGCCCC TGCCATCCCG GAACTCAGTG 
CCCATACTGA GGGCCTTTGG GGTCACCGAT GAAGGCTTCT CCGTCTGCTG CCACATACAG 
GGCTTTGCCC CCTACTTCTA CACCCCCGCG CCTCCTGGTT TTGGGGCCGA GCACCTGAGT 
GAGCTGCAGC AGGAGCTGAA CGCAGCCATC AGCCGGGACC AGCGCGGTGG GAAGGAGCTC 
TCAGGGCCGG CAGTGCTGGC AATAGAGCTA TGCTCCCGTG AGAGCATGTT TGGGTACCAC 
GGTCATGGCC CTTCTCCATT TCTCCGCATC ACCCTGGCAC TACCCCGCCT TATGGCACCA 
GCCCGCCGCC TTCTGGAACA GGGTGTCCGA GTGCCAGGCC TGGGCACCCC GAGCTTCGCA 
CCCTACGAAG CCAACGTGGA CTTTGAGATC CGGTTCATGG TGGATGCTGA CATTGTGGGA 
TGCAACTGGT TGGAGCTGCC AGCTGGAAAG TACGTTCGGA GGGCGGAGAA GAAGGCCACC 
CTGTGTCAGC TGGAGGTGGA CGTGCTGTGG TCAGATGTGA TCAGTCACCC ACCGGAGGGG 
CAGTGGCAGC GCATTGCACC CCTGCGTGTG CTTAGCTTCG ACATCGAGTG TGCTGGCCGA 
AAAGGCATCT TCCCTGAGCC TGAGCGTGAC CCCGTGATCC AGATCTGTTC TCTGGGGCTG 
CGCTGGGGGG AGCCGGAGCC ATTCTTGCGT CTGGCACTCA CGCTGCGGCC CTGTGCCCCC 
ATCCTGGGTG CCAAAGTGCA GAGCTATGAG CGGGAAGAAG ACCTGCTCCA GGCCTGGGCC 
GACTTCATCC TTGCCATGGA CCCTGACGTG ATCACCGGCT ACAACATTCA GAACTTTGAC 
CTCCCATACC TCATCTCTCG GGCACAGGCC CTAAAGGTGG ACCGCTTCCC TTTCCTGGGC 
CGCGTGACTG GTCTCCGCTC CAACATCCGT GACTCCTCCT TCCAATCAAG GCAGGTCGGC 
CGGCGGGACA GTAAGGTGAT CAGCATGGTG GGTCGCGTTC AGATGGATAT GCTGCAGGTG 
CTGCTTCGGG AACACAAGCT CCGCTCCTAC ACGCTCAACG CTGTGAGTTT CCACTTCCTG 
GGCGAGCAGA AGGAGGACGT TCAGCACAGC ATCATCACCG ACCTGCAGAA TGGGAACGAA 
CAGACGCGCC GCCGCCTGGC CGTGTACTGC CTGAAGGACG CCTTTCTGCC ACTCCGACTA 
CTAGAGCGCC TTATGGTGCT GGTGAATAAT GTGGAGATGG CGCGTGTCAC GGGTGTACCC 
CTTGGGTACC TGCTCACCCG GGGCCAGCAG GTCAAGGTCG TGTCTCAGCT GCTGCGCCAG 
GCCATGCGCC AGGGGCTGCT GATGCCTGTG GTGAAGACCG AGGGCAGTGA GGACTACACG 
GGAGCCACAG TCATTGAGCC CCTCAAAGGG TACTATGACG TCCCCATTGC CACCCTGGAC 
TTCTCCTCCT TGTACCCATC CATCATGATG GCCCATAATC TGTGCTACAC CACGCTGCTC 
CGACCTGGGG CTGCCCAGAA GCTGGGCCTT AAACCAGATG AGTTCATCAA GACACCCACT 
GGGGATGAGT TTGTGAAGTC ATCTGTACGG AAGGGCCTCC TGCCCCAGAT CCTGGAGAAT 
CTGCTGAGTG CCCGCAAGAG GGCCAAGGCT GAGCTGGCTC AGGAGACGGA CCCCCTGCGG 
CGACAGGTCT TGGACGGCCG GCAACTGGCA CTAAAAGTGA GTGCCAACTC CGTATATGGC 
TTCACTGGTG CCCAGGTGGG CAAGCTGCCA TGTTTGGAGA TCTCCCAGAG TGTCACTGGG 
TTCGGGCGGC AGATGATTGA GAAAACCAAG CAGCTTGTGG AGTCCAAGTA CACCGTGGAA 
AATGGCTACG ATGCCAACGC CAAGGTAGTC TACGGTGACA CGGACTCTGT GATGTGCCGG 
TTTGGCGTCT CCTCTGTGGC TGAAGCAATG TCTCTGGGGC GGGAGGCTGC AAACTGGGTA 
TCCAGTCACT TCCCATCACC CATCCGGCTG GAGTTCGAGA AGGTTTACTT CCCATACCTG 
CTCATCAGCA AGAAGCGCTA TGCTGGCCTG CTCTTCTCCT CCCGCTCTGA TGCCCATGAC 
AAAATGGACT GCAAGGGCCT GGAGGCTGTG CGCAGGGACA ACTGTCCCCT GGTGGCCAAC 
CTCGTTACAT CCTCTCTGCG CCGGATCCTC GTGGACCGGG ACCCTGATGG GGCAGTAGCC 
CATGCCAAGG ACGTCATCTC GGACCTGCTG TGCAACCGCA TAGACATCTC CCAGCTGGTC 
ATCACCAAAG AGTTGACCCG CGCAGCAGCA GACTATGCTG GCAAGCAGGC TCACGTGGAG 
CTGGCTGAGA GGATGAGGAA GCGCGACCCC GGCAGTGCGC CCAGCCTGGG TGACCGAGTC 
CCCTATGTGA TCATTGGTGC TGCTAAGGGT GTGGCCGCCT ACATGAAGTC GGAGGACCCC 
CTGTTTGTGC TGGAGCACAG CCTGCCCATC GACACTCAGT ACTACCTGGA GCAGCAGCTG 
GCCAAGCCGC TCTTGCGCAT CTTTGAGCCC ATCCTGGGTG AGGGCCGTGC AGAGTCTGTG 
CTGCTGCGCG GTGACCACAC ACGATGCAAG ACTGTGCTCA CCAGCAAGGT GGGCGGCCTC 
TTGGCCTTCA CCAAGCGCCG CAACTGTTGC ATTGGCTGCC GCTCCGTAAT CGACCATCAA 
GGAGCCGTGT GTAAGTTCTG TCAGCCACGG GAGTCGGAGC TCTATCAGAA GGAGGTGTCA 
CACCTGAATG CCTTGGAAGA ACGGTTCTCT CGCCTCTGGA CACAGTGTCA ACGCTGCCAG 
GGCAGCTTGC ATGAGGACGT CATCTGTACC AGCCGTGACT GTCCCATCTT CTACATGCGC 
AAGAAGGTGC GCAAGGACCT GGAAGACCAG GAACGGCTGC TGCAGCGCTT TGGACCGCCC 
GGCCCTGAGG CCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pold1 ( NM_011131.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035261.3 Mus musculus polymerase (DNA directed), delta 1, catalytic subunit (Pold1), mRNA.

Price & Availability↑

CloneID	OMu07525
Clone ID Related Accession (Same CDS sequence)	XM_030242220.1 , NM_011131.3
Accession Version	NM_011131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	DNA polymerase delta catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ173955.1, AK167569.1 and BX634546.1. On Jul 23, 2009 this sequence version replaced NM_011131.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009128.1, AK146837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END##

ATGGATTGTA AGCGGCGACA AGGACCAGGC CCTGGGGTGC CCCCAAAGCG GGCTCGAGGG 
CACCTCTGGG ATGAGGACGA GCCTTCGCCG TCGCAGTTTG AGGCGAACCT GGCACTGCTG 
GAGGAAATAG AGGCTGAGAA CCGGCTGCAG GAGGCAGAGG AGGAGCTGCA GCTGCCCCCA 
GAGGGCACCG TGGGTGGGCA GTTTTCCACT GCAGACATTG ACCCTCGGTG GCGGCGGCCC 
ACCCTACGTG CCCTGGACCC CAGCACGGAG CCCCTCATCT TCCAGCAGCT GGAGATTGAC 
CACTATGTGG GCTCAGCACC ACCCCTGCCA GAAGGGCCCC TGCCATCCCG GAACTCAGTG 
CCCATACTGA GGGCCTTTGG GGTCACCGAT GAAGGCTTCT CCGTCTGCTG CCACATACAG 
GGCTTTGCCC CCTACTTCTA CACCCCCGCG CCTCCTGGTT TTGGGGCCGA GCACCTGAGT 
GAGCTGCAGC AGGAGCTGAA CGCAGCCATC AGCCGGGACC AGCGCGGTGG GAAGGAGCTC 
TCAGGGCCGG CAGTGCTGGC AATAGAGCTA TGCTCCCGTG AGAGCATGTT TGGGTACCAC 
GGTCATGGCC CTTCTCCATT TCTCCGCATC ACCCTGGCAC TACCCCGCCT TATGGCACCA 
GCCCGCCGCC TTCTGGAACA GGGTGTCCGA GTGCCAGGCC TGGGCACCCC GAGCTTCGCA 
CCCTACGAAG CCAACGTGGA CTTTGAGATC CGGTTCATGG TGGATGCTGA CATTGTGGGA 
TGCAACTGGT TGGAGCTGCC AGCTGGAAAG TACGTTCGGA GGGCGGAGAA GAAGGCCACC 
CTGTGTCAGC TGGAGGTGGA CGTGCTGTGG TCAGATGTGA TCAGTCACCC ACCGGAGGGG 
CAGTGGCAGC GCATTGCACC CCTGCGTGTG CTTAGCTTCG ACATCGAGTG TGCTGGCCGA 
AAAGGCATCT TCCCTGAGCC TGAGCGTGAC CCCGTGATCC AGATCTGTTC TCTGGGGCTG 
CGCTGGGGGG AGCCGGAGCC ATTCTTGCGT CTGGCACTCA CGCTGCGGCC CTGTGCCCCC 
ATCCTGGGTG CCAAAGTGCA GAGCTATGAG CGGGAAGAAG ACCTGCTCCA GGCCTGGGCC 
GACTTCATCC TTGCCATGGA CCCTGACGTG ATCACCGGCT ACAACATTCA GAACTTTGAC 
CTCCCATACC TCATCTCTCG GGCACAGGCC CTAAAGGTGG ACCGCTTCCC TTTCCTGGGC 
CGCGTGACTG GTCTCCGCTC CAACATCCGT GACTCCTCCT TCCAATCAAG GCAGGTCGGC 
CGGCGGGACA GTAAGGTGAT CAGCATGGTG GGTCGCGTTC AGATGGATAT GCTGCAGGTG 
CTGCTTCGGG AACACAAGCT CCGCTCCTAC ACGCTCAACG CTGTGAGTTT CCACTTCCTG 
GGCGAGCAGA AGGAGGACGT TCAGCACAGC ATCATCACCG ACCTGCAGAA TGGGAACGAA 
CAGACGCGCC GCCGCCTGGC CGTGTACTGC CTGAAGGACG CCTTTCTGCC ACTCCGACTA 
CTAGAGCGCC TTATGGTGCT GGTGAATAAT GTGGAGATGG CGCGTGTCAC GGGTGTACCC 
CTTGGGTACC TGCTCACCCG GGGCCAGCAG GTCAAGGTCG TGTCTCAGCT GCTGCGCCAG 
GCCATGCGCC AGGGGCTGCT GATGCCTGTG GTGAAGACCG AGGGCAGTGA GGACTACACG 
GGAGCCACAG TCATTGAGCC CCTCAAAGGG TACTATGACG TCCCCATTGC CACCCTGGAC 
TTCTCCTCCT TGTACCCATC CATCATGATG GCCCATAATC TGTGCTACAC CACGCTGCTC 
CGACCTGGGG CTGCCCAGAA GCTGGGCCTT AAACCAGATG AGTTCATCAA GACACCCACT 
GGGGATGAGT TTGTGAAGTC ATCTGTACGG AAGGGCCTCC TGCCCCAGAT CCTGGAGAAT 
CTGCTGAGTG CCCGCAAGAG GGCCAAGGCT GAGCTGGCTC AGGAGACGGA CCCCCTGCGG 
CGACAGGTCT TGGACGGCCG GCAACTGGCA CTAAAAGTGA GTGCCAACTC CGTATATGGC 
TTCACTGGTG CCCAGGTGGG CAAGCTGCCA TGTTTGGAGA TCTCCCAGAG TGTCACTGGG 
TTCGGGCGGC AGATGATTGA GAAAACCAAG CAGCTTGTGG AGTCCAAGTA CACCGTGGAA 
AATGGCTACG ATGCCAACGC CAAGGTAGTC TACGGTGACA CGGACTCTGT GATGTGCCGG 
TTTGGCGTCT CCTCTGTGGC TGAAGCAATG TCTCTGGGGC GGGAGGCTGC AAACTGGGTA 
TCCAGTCACT TCCCATCACC CATCCGGCTG GAGTTCGAGA AGGTTTACTT CCCATACCTG 
CTCATCAGCA AGAAGCGCTA TGCTGGCCTG CTCTTCTCCT CCCGCTCTGA TGCCCATGAC 
AAAATGGACT GCAAGGGCCT GGAGGCTGTG CGCAGGGACA ACTGTCCCCT GGTGGCCAAC 
CTCGTTACAT CCTCTCTGCG CCGGATCCTC GTGGACCGGG ACCCTGATGG GGCAGTAGCC 
CATGCCAAGG ACGTCATCTC GGACCTGCTG TGCAACCGCA TAGACATCTC CCAGCTGGTC 
ATCACCAAAG AGTTGACCCG CGCAGCAGCA GACTATGCTG GCAAGCAGGC TCACGTGGAG 
CTGGCTGAGA GGATGAGGAA GCGCGACCCC GGCAGTGCGC CCAGCCTGGG TGACCGAGTC 
CCCTATGTGA TCATTGGTGC TGCTAAGGGT GTGGCCGCCT ACATGAAGTC GGAGGACCCC 
CTGTTTGTGC TGGAGCACAG CCTGCCCATC GACACTCAGT ACTACCTGGA GCAGCAGCTG 
GCCAAGCCGC TCTTGCGCAT CTTTGAGCCC ATCCTGGGTG AGGGCCGTGC AGAGTCTGTG 
CTGCTGCGCG GTGACCACAC ACGATGCAAG ACTGTGCTCA CCAGCAAGGT GGGCGGCCTC 
TTGGCCTTCA CCAAGCGCCG CAACTGTTGC ATTGGCTGCC GCTCCGTAAT CGACCATCAA 
GGAGCCGTGT GTAAGTTCTG TCAGCCACGG GAGTCGGAGC TCTATCAGAA GGAGGTGTCA 
CACCTGAATG CCTTGGAAGA ACGGTTCTCT CGCCTCTGGA CACAGTGTCA ACGCTGCCAG 
GGCAGCTTGC ATGAGGACGT CATCTGTACC AGCCGTGACT GTCCCATCTT CTACATGCGC 
AAGAAGGTGC GCAAGGACCT GGAAGACCAG GAACGGCTGC TGCAGCGCTT TGGACCGCCC 
GGCCCTGAGG CCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035261.3
CDS	62..3379
Translation	MDCKRRQGPGPGVPPKRARGHLWDEDEPSPSQFEANLALLEEIEAENRLQEAEEELQLPPEGTVGGQFSTADIDPRWRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVIDHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFGPPGPEAW

Target ORF information:

RefSeq Version	NM_011131.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), delta 1, catalytic subunit (Pold1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011131.3

ORF Insert Sequence:

ATGGATTGTA AGCGGCGACA AGGACCAGGC CCTGGGGTGC CCCCAAAGCG GGCTCGAGGG 
CACCTCTGGG ATGAGGACGA GCCTTCGCCG TCGCAGTTTG AGGCGAACCT GGCACTGCTG 
GAGGAAATAG AGGCTGAGAA CCGGCTGCAG GAGGCAGAGG AGGAGCTGCA GCTGCCCCCA 
GAGGGCACCG TGGGTGGGCA GTTTTCCACT GCAGACATTG ACCCTCGGTG GCGGCGGCCC 
ACCCTACGTG CCCTGGACCC CAGCACGGAG CCCCTCATCT TCCAGCAGCT GGAGATTGAC 
CACTATGTGG GCTCAGCACC ACCCCTGCCA GAAGGGCCCC TGCCATCCCG GAACTCAGTG 
CCCATACTGA GGGCCTTTGG GGTCACCGAT GAAGGCTTCT CCGTCTGCTG CCACATACAG 
GGCTTTGCCC CCTACTTCTA CACCCCCGCG CCTCCTGGTT TTGGGGCCGA GCACCTGAGT 
GAGCTGCAGC AGGAGCTGAA CGCAGCCATC AGCCGGGACC AGCGCGGTGG GAAGGAGCTC 
TCAGGGCCGG CAGTGCTGGC AATAGAGCTA TGCTCCCGTG AGAGCATGTT TGGGTACCAC 
GGTCATGGCC CTTCTCCATT TCTCCGCATC ACCCTGGCAC TACCCCGCCT TATGGCACCA 
GCCCGCCGCC TTCTGGAACA GGGTGTCCGA GTGCCAGGCC TGGGCACCCC GAGCTTCGCA 
CCCTACGAAG CCAACGTGGA CTTTGAGATC CGGTTCATGG TGGATGCTGA CATTGTGGGA 
TGCAACTGGT TGGAGCTGCC AGCTGGAAAG TACGTTCGGA GGGCGGAGAA GAAGGCCACC 
CTGTGTCAGC TGGAGGTGGA CGTGCTGTGG TCAGATGTGA TCAGTCACCC ACCGGAGGGG 
CAGTGGCAGC GCATTGCACC CCTGCGTGTG CTTAGCTTCG ACATCGAGTG TGCTGGCCGA 
AAAGGCATCT TCCCTGAGCC TGAGCGTGAC CCCGTGATCC AGATCTGTTC TCTGGGGCTG 
CGCTGGGGGG AGCCGGAGCC ATTCTTGCGT CTGGCACTCA CGCTGCGGCC CTGTGCCCCC 
ATCCTGGGTG CCAAAGTGCA GAGCTATGAG CGGGAAGAAG ACCTGCTCCA GGCCTGGGCC 
GACTTCATCC TTGCCATGGA CCCTGACGTG ATCACCGGCT ACAACATTCA GAACTTTGAC 
CTCCCATACC TCATCTCTCG GGCACAGGCC CTAAAGGTGG ACCGCTTCCC TTTCCTGGGC 
CGCGTGACTG GTCTCCGCTC CAACATCCGT GACTCCTCCT TCCAATCAAG GCAGGTCGGC 
CGGCGGGACA GTAAGGTGAT CAGCATGGTG GGTCGCGTTC AGATGGATAT GCTGCAGGTG 
CTGCTTCGGG AACACAAGCT CCGCTCCTAC ACGCTCAACG CTGTGAGTTT CCACTTCCTG 
GGCGAGCAGA AGGAGGACGT TCAGCACAGC ATCATCACCG ACCTGCAGAA TGGGAACGAA 
CAGACGCGCC GCCGCCTGGC CGTGTACTGC CTGAAGGACG CCTTTCTGCC ACTCCGACTA 
CTAGAGCGCC TTATGGTGCT GGTGAATAAT GTGGAGATGG CGCGTGTCAC GGGTGTACCC 
CTTGGGTACC TGCTCACCCG GGGCCAGCAG GTCAAGGTCG TGTCTCAGCT GCTGCGCCAG 
GCCATGCGCC AGGGGCTGCT GATGCCTGTG GTGAAGACCG AGGGCAGTGA GGACTACACG 
GGAGCCACAG TCATTGAGCC CCTCAAAGGG TACTATGACG TCCCCATTGC CACCCTGGAC 
TTCTCCTCCT TGTACCCATC CATCATGATG GCCCATAATC TGTGCTACAC CACGCTGCTC 
CGACCTGGGG CTGCCCAGAA GCTGGGCCTT AAACCAGATG AGTTCATCAA GACACCCACT 
GGGGATGAGT TTGTGAAGTC ATCTGTACGG AAGGGCCTCC TGCCCCAGAT CCTGGAGAAT 
CTGCTGAGTG CCCGCAAGAG GGCCAAGGCT GAGCTGGCTC AGGAGACGGA CCCCCTGCGG 
CGACAGGTCT TGGACGGCCG GCAACTGGCA CTAAAAGTGA GTGCCAACTC CGTATATGGC 
TTCACTGGTG CCCAGGTGGG CAAGCTGCCA TGTTTGGAGA TCTCCCAGAG TGTCACTGGG 
TTCGGGCGGC AGATGATTGA GAAAACCAAG CAGCTTGTGG AGTCCAAGTA CACCGTGGAA 
AATGGCTACG ATGCCAACGC CAAGGTAGTC TACGGTGACA CGGACTCTGT GATGTGCCGG 
TTTGGCGTCT CCTCTGTGGC TGAAGCAATG TCTCTGGGGC GGGAGGCTGC AAACTGGGTA 
TCCAGTCACT TCCCATCACC CATCCGGCTG GAGTTCGAGA AGGTTTACTT CCCATACCTG 
CTCATCAGCA AGAAGCGCTA TGCTGGCCTG CTCTTCTCCT CCCGCTCTGA TGCCCATGAC 
AAAATGGACT GCAAGGGCCT GGAGGCTGTG CGCAGGGACA ACTGTCCCCT GGTGGCCAAC 
CTCGTTACAT CCTCTCTGCG CCGGATCCTC GTGGACCGGG ACCCTGATGG GGCAGTAGCC 
CATGCCAAGG ACGTCATCTC GGACCTGCTG TGCAACCGCA TAGACATCTC CCAGCTGGTC 
ATCACCAAAG AGTTGACCCG CGCAGCAGCA GACTATGCTG GCAAGCAGGC TCACGTGGAG 
CTGGCTGAGA GGATGAGGAA GCGCGACCCC GGCAGTGCGC CCAGCCTGGG TGACCGAGTC 
CCCTATGTGA TCATTGGTGC TGCTAAGGGT GTGGCCGCCT ACATGAAGTC GGAGGACCCC 
CTGTTTGTGC TGGAGCACAG CCTGCCCATC GACACTCAGT ACTACCTGGA GCAGCAGCTG 
GCCAAGCCGC TCTTGCGCAT CTTTGAGCCC ATCCTGGGTG AGGGCCGTGC AGAGTCTGTG 
CTGCTGCGCG GTGACCACAC ACGATGCAAG ACTGTGCTCA CCAGCAAGGT GGGCGGCCTC 
TTGGCCTTCA CCAAGCGCCG CAACTGTTGC ATTGGCTGCC GCTCCGTAAT CGACCATCAA 
GGAGCCGTGT GTAAGTTCTG TCAGCCACGG GAGTCGGAGC TCTATCAGAA GGAGGTGTCA 
CACCTGAATG CCTTGGAAGA ACGGTTCTCT CGCCTCTGGA CACAGTGTCA ACGCTGCCAG 
GGCAGCTTGC ATGAGGACGT CATCTGTACC AGCCGTGACT GTCCCATCTT CTACATGCGC 
AAGAAGGTGC GCAAGGACCT GGAAGACCAG GAACGGCTGC TGCAGCGCTT TGGACCGCCC 
GGCCCTGAGG CCTGGTGA