Plod1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plod1
Entrez Gene ID	18822
Full Name	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
Synonyms	2410042F05Rik,AI854890,AV007929,Lh1,Plod
General protein information	Preferred Names procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 Names procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E2\|4 78.57 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011122.3	NP_035252.1	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLOD1	XP_001142788.1
Canis lupus familiaris (dog)	PLOD1	XP_544565.2
Danio rerio (zebrafish)	plod1a	NP_001071210.2
Bos taurus (cattle)	PLOD1	NP_776573.1
Rattus norvegicus (Norway rat)	Plod1	NP_446279.2
Xenopus tropicalis (tropical clawed frog)	plod1	NP_001072340.1
Homo sapiens (human)	PLOD1	NP_000293.2
Mus musculus (house mouse)	Plod1	NP_035252.1
Gallus gallus (chicken)	PLOD1	NP_001005618.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
Heard ME, Besio R, Weis M, Rai J, Hudson DM, Dimori M, Zimmerman SM, Kamykowski JA, Hogue WR, Swain FL, Burdine MS, Mackintosh SG, Tackett AJ, Suva LJ, Eyre DR, Morello R
PLoS genetics12(4)e1006002(2016 Apr)

Endothelial cells regulate neural crest and second heart field morphogenesis.
Milgrom-Hoffman M, Michailovici I, Ferrara N, Zelzer E, Tzahor E
Biology open3(8)679-88(2014 Jul)

Collagen XIV is important for growth and structural integrity of the myocardium.
Tao G, Levay AK, Peacock JD, Huk DJ, Both SN, Purcell NH, Pinto JR, Galantowicz ML, Koch M, Lucchesi PA, Birk DE, Lincoln J
Journal of molecular and cellular cardiology53(5)626-38(2012 Nov)

The matrisome: in silico definition and in vivo characterization by proteomics of normal and tumor extracellular matrices.
Naba A, Clauser KR, Hoersch S, Liu H, Carr SA, Hynes RO
Molecular & cellular proteomics : MCP11(4)M111.014647(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Plod1(-/-) mice offer an in vivo model for systematic analysis of the tissue-specific consequences of the lack of LH1 activity
Title: Tissue-specific changes in the hydroxylysine content and cross-links of collagens and alterations in fibril morphology in lysyl hydroxylase 1 knock-out mice.

The following Plod1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plod1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07514	NM_011122.3 Latest version!	Mus musculus procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (Plod1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plod1 ( NM_011122.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035252.1 Mus musculus procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (Plod1), mRNA.

Price & Availability↑

CloneID	OMu07514
Clone ID Related Accession (Same CDS sequence)	NM_011122.3
Accession Version	NM_011122.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY312508.1, AK137480.1, AK003529.1 and AI834765.1. On Apr 28, 2009 this sequence version replaced NM_011122.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK137480.1, AF046782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC TGCTGCTCCT GGCCCCGTTG GCCTGGCTTC TTCTGGTCCA GGCGAAGGAT 
GACGCCAAGC TAGAGGACAA CCTTCTGGTC CTCACTGTGG CCACAAAAGA GACTGAGGGC 
TTCCGCCGCT TCAAGCGTTC AGCTCAGTTC TTCAACTACA AGATCCAGTC ACTGGGCCTG 
GGGGAGGACT GGAGTGTGGA TGGAGGACCA GCAGCAGCAG GTGGAGGACA GAAGGTTCGG 
CTGCTGAAGA AGGCACTGGA AAAGCACGCA GACAAGGAAG ACCTGGTTAT TCTCTTCGTT 
GACAGTTATG ACGTGGTGTT TGCCTCGGGA CCCCGGGAAC TCTTAAAGAA GTTCCAGCAG 
GCCAAGAGCC AGGTGGTCTT CTCCGCAGAG GAGCATATCT ACCCGGACCG GAGGCTGGAG 
GCCAAGTATC CCACAGTCCC CGACGGCAAG CGGTTCCTGG GCTCTGGAGG CTTCATTGGT 
TATGCCCCCA GCCTCAGCAA ACTGGTGGCC GAGTGGGAAG GCCAGGACAG TGACAGCGAC 
CAGCTCTTTT ATACCAAGAT CTTCTTGAAC CCGGAGAAGA GGGAGCAAAT CAATATCAGC 
CTGGACCATC GCTGCCGAAT CTTCCAGAAC CTGGATGGAG CCTTGGATGA AGTTGTGCTC 
AAGTTTGAAA TGGGCCACGT GAGAGCACGA AATCTGGCCT ATGACACCCT CCCAGTGGTC 
GTTCATGGCA ATGGGCCCAC CAAGCTGCAG CTGAACTACC TGGGCAACTA CATTCCTCGA 
TTCTGGACCT TCGAGACGGG CTGCACCGTG TGTGACGAGG GCCTACGGAG CCTCAAGGGC 
ATTGGGGATG AAGCTCTCCC CACTGTCCTG GTCGGTGTGT TCATTGAGCA GCCCACACCA 
TTTCTATCCT TGTTTTTCCT GCGGCTGCTG CGCCTACGAT ACCCTCAGAA ACAGATGCGG 
CTCTTCATTC ACAACCAGGA ACGGCACCAC AAACTTCAGG TAGAGCAGTT CCTGGCGGAG 
CACGGCAGTG AGTACCAGTC TGTGAAGCTA GTGGGTCCTG AGGTGCGCAT GGCCAACGCC 
GACGCCAGGA ACATGGGCGC AGACCTGTGC CGTCAGGACC AAACTTGCAC CTACTACTTC 
AGTGTGGATG CTGATGTGGC TCTGACGGAG CCGAACAGCC TAAGGCTCCT GATTGAACAA 
AACAAGAATG TCATTGCGCC ACTCATGACC CGCCACGGGA GGCTCTGGTC CAACTTCTGG 
GGGGGTCTGA GTGCAGATGG GTACTACGCC CGCTCAGAGG ACTACGTGGA CATTGTGCAG 
GGCCGTCGTG TTGGCGTCTG GAACGTGCCT TACATCTCAA ACATCTACCT GATCAAGGGC 
AGTGCCCTGC GGGCTGAACT GCAGAACGTA GACCTTTTCC ACTACAGCAA GCTGGATTCC 
GACATGAGTT TCTGCGCCAA TGTCCGACAG CAGGAGGTGT TCATGTTCCT GACCAACCGG 
CACACCTTTG GCCACCTGCT TTCCCTGGAT AACTACCAGA CCACCCACCT ACATAATGAT 
CTCTGGGAGG TGTTCAGCAA CCCTGAGGAC TGGAAAGAAA AGTACATCCA TGAGAATTAC 
ACCAAGGCCC TGGCGGGGAA GCTGGTGGAG ACGCCTTGTC CGGATGTCTA CTGGTTCCCC 
ATCTTCACGG AGGCGGCCTG TGATGAGCTG GTGGAGGAGA TGGAACACTA TGGCCAGTGG 
TCTCTGGGTG ATAATAAGGA CAACCGGATC CAGGGTGGCT ACGAAAACGT GCCCACTATC 
GACATCCATA TGAACCAGAT CACCTTCGAG CGGGAGTGGC ACAAGTTCCT GGTGGAGTAC 
ATCGCCCCCA TGACAGAGAA GCTGTACCCT GGCTACTACA CTAGGGCCCA GTTTGATCTA 
GCCTTCGTCG TCCGCTATAA GCCCGATGAG CAGCCTTCCT TGATGCCCCA CCATGACGCC 
TCTACCTTCA CCGTCAACAT AGCCCTGAAC AGGGTTGGGG AAGATTATGA GGGCGGAGGT 
TGCCGATTTC TGCGCTACAA CTGCTCCGTG AGGGCACCAA GGAAGGGCTG GGCCCTCCTG 
CACCCCGGGC GGCTCACACA CTATCACGAG GGGCTTCCTA CTACCAAGGG CACGCGCTAC 
ATTGCTGTGT CTTTCGTCGA TCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035252.1
CDS	100..2286
Translation	MRSLLLLAPLAWLLLVQAKDDAKLEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQSLGLGEDWSVDGGPAAAGGGQKVRLLKKALEKHADKEDLVILFVDSYDVVFASGPRELLKKFQQAKSQVVFSAEEHIYPDRRLEAKYPTVPDGKRFLGSGGFIGYAPSLSKLVAEWEGQDSDSDQLFYTKIFLNPEKREQINISLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVVVHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFLSLFFLRLLRLRYPQKQMRLFIHNQERHHKLQVEQFLAEHGSEYQSVKLVGPEVRMANADARNMGADLCRQDQTCTYYFSVDADVALTEPNSLRLLIEQNKNVIAPLMTRHGRLWSNFWGGLSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRAELQNVDLFHYSKLDSDMSFCANVRQQEVFMFLTNRHTFGHLLSLDNYQTTHLHNDLWEVFSNPEDWKEKYIHENYTKALAGKLVETPCPDVYWFPIFTEAACDELVEEMEHYGQWSLGDNKDNRIQGGYENVPTIDIHMNQITFEREWHKFLVEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTVNIALNRVGEDYEGGGCRFLRYNCSVRAPRKGWALLHPGRLTHYHEGLPTTKGTRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_011122.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (Plod1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011122.3

ORF Insert Sequence:

ATGCGGTCCC TGCTGCTCCT GGCCCCGTTG GCCTGGCTTC TTCTGGTCCA GGCGAAGGAT 
GACGCCAAGC TAGAGGACAA CCTTCTGGTC CTCACTGTGG CCACAAAAGA GACTGAGGGC 
TTCCGCCGCT TCAAGCGTTC AGCTCAGTTC TTCAACTACA AGATCCAGTC ACTGGGCCTG 
GGGGAGGACT GGAGTGTGGA TGGAGGACCA GCAGCAGCAG GTGGAGGACA GAAGGTTCGG 
CTGCTGAAGA AGGCACTGGA AAAGCACGCA GACAAGGAAG ACCTGGTTAT TCTCTTCGTT 
GACAGTTATG ACGTGGTGTT TGCCTCGGGA CCCCGGGAAC TCTTAAAGAA GTTCCAGCAG 
GCCAAGAGCC AGGTGGTCTT CTCCGCAGAG GAGCATATCT ACCCGGACCG GAGGCTGGAG 
GCCAAGTATC CCACAGTCCC CGACGGCAAG CGGTTCCTGG GCTCTGGAGG CTTCATTGGT 
TATGCCCCCA GCCTCAGCAA ACTGGTGGCC GAGTGGGAAG GCCAGGACAG TGACAGCGAC 
CAGCTCTTTT ATACCAAGAT CTTCTTGAAC CCGGAGAAGA GGGAGCAAAT CAATATCAGC 
CTGGACCATC GCTGCCGAAT CTTCCAGAAC CTGGATGGAG CCTTGGATGA AGTTGTGCTC 
AAGTTTGAAA TGGGCCACGT GAGAGCACGA AATCTGGCCT ATGACACCCT CCCAGTGGTC 
GTTCATGGCA ATGGGCCCAC CAAGCTGCAG CTGAACTACC TGGGCAACTA CATTCCTCGA 
TTCTGGACCT TCGAGACGGG CTGCACCGTG TGTGACGAGG GCCTACGGAG CCTCAAGGGC 
ATTGGGGATG AAGCTCTCCC CACTGTCCTG GTCGGTGTGT TCATTGAGCA GCCCACACCA 
TTTCTATCCT TGTTTTTCCT GCGGCTGCTG CGCCTACGAT ACCCTCAGAA ACAGATGCGG 
CTCTTCATTC ACAACCAGGA ACGGCACCAC AAACTTCAGG TAGAGCAGTT CCTGGCGGAG 
CACGGCAGTG AGTACCAGTC TGTGAAGCTA GTGGGTCCTG AGGTGCGCAT GGCCAACGCC 
GACGCCAGGA ACATGGGCGC AGACCTGTGC CGTCAGGACC AAACTTGCAC CTACTACTTC 
AGTGTGGATG CTGATGTGGC TCTGACGGAG CCGAACAGCC TAAGGCTCCT GATTGAACAA 
AACAAGAATG TCATTGCGCC ACTCATGACC CGCCACGGGA GGCTCTGGTC CAACTTCTGG 
GGGGGTCTGA GTGCAGATGG GTACTACGCC CGCTCAGAGG ACTACGTGGA CATTGTGCAG 
GGCCGTCGTG TTGGCGTCTG GAACGTGCCT TACATCTCAA ACATCTACCT GATCAAGGGC 
AGTGCCCTGC GGGCTGAACT GCAGAACGTA GACCTTTTCC ACTACAGCAA GCTGGATTCC 
GACATGAGTT TCTGCGCCAA TGTCCGACAG CAGGAGGTGT TCATGTTCCT GACCAACCGG 
CACACCTTTG GCCACCTGCT TTCCCTGGAT AACTACCAGA CCACCCACCT ACATAATGAT 
CTCTGGGAGG TGTTCAGCAA CCCTGAGGAC TGGAAAGAAA AGTACATCCA TGAGAATTAC 
ACCAAGGCCC TGGCGGGGAA GCTGGTGGAG ACGCCTTGTC CGGATGTCTA CTGGTTCCCC 
ATCTTCACGG AGGCGGCCTG TGATGAGCTG GTGGAGGAGA TGGAACACTA TGGCCAGTGG 
TCTCTGGGTG ATAATAAGGA CAACCGGATC CAGGGTGGCT ACGAAAACGT GCCCACTATC 
GACATCCATA TGAACCAGAT CACCTTCGAG CGGGAGTGGC ACAAGTTCCT GGTGGAGTAC 
ATCGCCCCCA TGACAGAGAA GCTGTACCCT GGCTACTACA CTAGGGCCCA GTTTGATCTA 
GCCTTCGTCG TCCGCTATAA GCCCGATGAG CAGCCTTCCT TGATGCCCCA CCATGACGCC 
TCTACCTTCA CCGTCAACAT AGCCCTGAAC AGGGTTGGGG AAGATTATGA GGGCGGAGGT 
TGCCGATTTC TGCGCTACAA CTGCTCCGTG AGGGCACCAA GGAAGGGCTG GGCCCTCCTG 
CACCCCGGGC GGCTCACACA CTATCACGAG GGGCTTCCTA CTACCAAGGG CACGCGCTAC 
ATTGCTGTGT CTTTCGTCGA TCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
PLoS genetics12(4)e1006002(2016 Apr)
Heard ME,Besio R,Weis M,Rai J,Hudson DM,Dimori M,Zimmerman SM,Kamykowski JA,Hogue WR,Swain FL,Burdine MS,Mackintosh SG,Tackett AJ,Suva LJ,Eyre DR,Morello R

Endothelial cells regulate neural crest and second heart field morphogenesis.
Biology open3(8)679-88(2014 Jul)
Milgrom-Hoffman M,Michailovici I,Ferrara N,Zelzer E,Tzahor E

Collagen XIV is important for growth and structural integrity of the myocardium.
Journal of molecular and cellular cardiology53(5)626-38(2012 Nov)
Tao G,Levay AK,Peacock JD,Huk DJ,Both SN,Purcell NH,Pinto JR,Galantowicz ML,Koch M,Lucchesi PA,Birk DE,Lincoln J

The matrisome: in silico definition and in vivo characterization by proteomics of normal and tumor extracellular matrices.
Molecular & cellular proteomics : MCP11(4)M111.014647(2012 Apr)
Naba A,Clauser KR,Hoersch S,Liu H,Carr SA,Hynes RO

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Plod1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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