Plcb3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plcb3
Entrez Gene ID	18797
Full Name	phospholipase C, beta 3
Synonyms	mKIAA4098
General protein information	Preferred Names phospholipase C, beta 3 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 PLC-beta-3 phosphoinositide phospholipase C-beta-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 5.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008874.4	NP_032900.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform b
XM_006526767.4	XP_006526830.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X2
XM_006526766.3	XP_006526829.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1
NM_001290349.1	NP_001277278.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform a
XM_006526767.3	XP_006526830.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X2
XM_006526766.4	XP_006526829.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	plcb3	NP_001116245.1
Pan troglodytes (chimpanzee)	PLCB3	XP_003313091.1
	PLCB3	XP_001115104.2
Bos taurus (cattle)	PLCB3	NP_001179352.1
Rattus norvegicus (Norway rat)	Plcb3	NP_203501.1
Xenopus tropicalis (tropical clawed frog)	plcb3	XP_002940270.2
Canis lupus familiaris (dog)	PLCB3	XP_005631608.1
Mus musculus (house mouse)	Plcb3	NP_032900.2
Homo sapiens (human)	PLCB3	NP_000923.1

Gene Ontology
Bibliography

Related articles in PubMed

The heterotrimeric G protein G?
Pradhan S, Khatlani T, Nairn AC, Vijayan KV
The Journal of biological chemistry292(32)13133-13142(2017 08)

Synergistic Signaling by Light and Acetylcholine in Mouse Iris Sphincter Muscle.
Wang Q, Yue WWS, Jiang Z, Xue T, Kang SH, Bergles DE, Mikoshiba K, Offermanns S, Yau KW
Current biology : CB27(12)1791-1800.e5(2017 Jun)

Gnb isoforms control a signaling pathway comprising Rac1, Plc?2, and Plc?3 leading to LFA-1 activation and neutrophil arrest in vivo.
Block H, Stadtmann A, Riad D, Rossaint J, Sohlbach C, Germena G, Wu D, Simon SI, Ley K, Zarbock A
Blood127(3)314-24(2016 Jan)

Macrophages monitor tissue osmolarity and induce inflammatory response through NLRP3 and NLRC4 inflammasome activation.
Ip WK, Medzhitov R
Nature communications66931(2015 May)

Critical role for mast cell Stat5 activity in skin inflammation.
Ando T, Xiao W, Gao P, Namiranian S, Matsumoto K, Tomimori Y, Hong H, Yamashita H, Kimura M, Kashiwakura J, Hata TR, Izuhara K, Gurish MF, Roers A, Rafaels NM, Barnes KC, Jamora C, Kawakami Y, Kawakami T
Cell reports6(2)366-76(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data, including data from studies using transgenic/knockout mice, suggest that Ppp1ca and Gnb1 interact in quiescent platelets; then, Ppp1ca and Plcb3 interact during platelet aggregation; thus, Gnb1 enlists Ppp1ca to modulate G protein-coupled receptor signaling. (Ppp1ca = protein phosphatase 1, catalytic subunit alpha; Gnb1 = guanine nucleotide-binding protein, subunit beta-1; Plcb3 = phospholipase C, subunit beta-3)
Title: The heterotrimeric G protein G?

Gnb isoforms control a signaling pathway comprising Rac1, Plcbeta2, and Plcbeta3 leading to LFA-1 activation and neutrophil arrest in vivo
Title: Gnb isoforms control a signaling pathway comprising Rac1, Plc?2, and Plc?3 leading to LFA-1 activation and neutrophil arrest in vivo.

Plcb3-deficient mice spontaneously develop atopic dermatitis-like skin lesions.
Title: Critical role for mast cell Stat5 activity in skin inflammation.

Data suggest that PLCbeta3 acts as a negative regulator of VEGF- (vascular endothelial growth factor A-) mediated vascular hyperpermeability through intracellular calcium signaling.
Title: Revealing the role of phospholipase C?3 in the regulation of VEGF-induced vascular permeability.

GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.
Title: GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.

The following Plcb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plcb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55309	XM_006526766.4 Latest version!	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu55310	XM_006526767.4 Latest version!	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13272	NM_008874.4 Latest version!	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu17008	NM_001290349.1 Latest version!	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu55310	XM_006526767.3	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu55309	XM_006526766.3	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plcb3 ( XM_006526766.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526829.1 Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55309
Clone ID Related Accession (Same CDS sequence)	XM_006526766.3 , XM_006526766.4
Accession Version	XM_006526766.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526766.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGGA CCCCCACTTC AGTGTTGCCT GACCACCTCA CCCCCTATTC CAGCCTTGTT 
GCACTCCGGA AACTTGAGTC CCCTGTTGTC CTGGAGGCCT CCAGTCGGAA CCTGGTGACC 
CTGCGTGTGG ACCCTAATGG CTTCTTCTTG TACTGGACAG GACCCAACAT GGAGGTGGAC 
ACACTGGACA TCAGCTCCAT CAGGGACACA AGGACAGGTC GTTATGCCCG CCTGCCCAAG 
GACCCTAAGA TTCGAGAAGT ACTGGGCTTT GGAGGTCCTG ACACCCGGCT GGAGGAGAAA 
CTGATGACAG TGGTGGCCGG GCCAGATCCA GTAAATACCA CATTCTTGAA CTTCATGGCC 
GTGCAAGATG ACACAGTCAA GGTCTGGTCA GAGGAGTTGT TTAAACTGGC CATGAACATA 
TTGGCTCAGA ACGCCTCCCG GAACACCTTC CTGCGGAAAG CATACACGAA GCTGAAGCTG 
CAGGTGAACC AGGATGGACG GATTCCGGTC AAGAACATTC TGAAGATGTT CTCGGCGGAC 
AAGAAGCGGG TGGAGACGGC GCTGGAGTCC TGTGGCCTCA ACTTCAACCG AAGTGAGTCC 
ATTCGCCCTG ATGAGTTTCC CTTGGAAATT TTTGAGCGGT TCTTGAATAA ACTGTGTCTG 
CGCCCGGATA TTGACAAGAT CCTGCTGGAG ATAGGTGCCA AGGGCAAGCC GTACCTCACT 
CTGGAGCAGC TCATGGACTT TATCAACCAG AAGCAGAGAG ACCCGAGACT CAACGAAGTG 
CTGTACCCGC CACTTCGGTC CTCACAGGCT CGGCTGCTCA TTGAGAAGTA CGAGACGAAC 
AAACAGTTCC TGGAGCGCGA CCAGATGTCT ATGGAGGGCT TCAGCCGCTA CCTGGGAGGG 
GAGGAGAATG GTATCCTGCC TCTGGAGGCC CTGGATCTGA GCATGGACAT GACCCAGCCA 
CTGAGCGCAT ATTTTATCAA CTCCTCACAC AACACCTATC TCACTGCGGG CCAGCTGGCT 
GGACCATCAT CGGTGGAGAT GTACCGCCAG GCACTGCTGT GGGGCTGCCG CTGTGTGGAG 
CTGGATGTAT GGAAGGGACG GCCACCGGAG GAAGAGCCTT TCATCACTCA TGGTTTCACC 
ATGACCACTG AGGTGCCATT GCGTGATGTG CTAGAGGCCA TCGCTGAGGC AGCCTTCAAG 
ACCTCGCCCT ACCCTGTCAT CCTCTCCTTT GAGAACCATG TCGACTCGGC AAAGCAGCAG 
GCCAAGATGG CTGAGTACTG CCGCTCTATC TTTGGGGATG CGCTGCTCAT TGACCCTCTC 
GACAAATACC CGCTATCTGC GGGCATCCCC CTGCCTAGTC CACAGGACCT GATGGGCCGT 
ATCCTGGTGA AGAATAAGAA GCGACATCGG CCCAGCACAG GTGTCCCTGA CAGCTCAGTG 
CGCAAGCGGC CTCTGGAACA GAGCAACTCG GCCTTGAGTG AGAGCTCGGC CGCCACAGAG 
CCCTCCTCAC CTCAGCTTGG GTCCCCCAGC TCCGACAGCT GCCCTGGCCT AAGCAATGGG 
GAGGAGGTGG GACTCGAGAA GACCAGCCTG GAGCCTCAGA AGTCTCTAGG TGAGGAGAGC 
CTCAGCCGGG AACCTAATGT GCCCATGCCT GACCGTGACC GAGAGGATGA GGAGGAAGAT 
GAAGAAGAAG AGGAAACAAC GGATCCAAAA AAGCCCACCA CTGATGAGGG CACAGCCAGC 
AGTGAGGTCA ATGCCACGGA GGAGATGTCA ACGCTCGTCA ACTACGTTGA GCCCGTCAAG 
TTCAAGTCCT TCGAGGCTGC TCGAAAAAGG AACAAGTGCT TCGAGATGTC ATCCTTCGTG 
GAGACCAAGG CAATGGAGCA ACTGACCAAG AGTCCTATGG AGTTTGTGGA ATACAACAAA 
CAGCAGCTCA GCCGCATCTA CCCTAAGGGC ACACGAGTGG ACTCCTCCAA CTACATGCCT 
CAGCTCTTCT GGAACGTGGG TTGCCAGCTT GTTGCCCTCA ACTTCCAGAC CTTGGATTTG 
CCAATGCAGC TCAACGCAGG CGTGTTTGAG TACAATGGGC GCAGCGGGTA CTTGCTCAAG 
CCCGAGTTTA TGCGGCGGCC GGACAAGTCT TTTGATCCCT TCACTGAGGT CATCGTAGAT 
GGCATAGTGG CCAACGCCTT GCGGGTCAAG GTGATTTCGG GGCAGTTCCT GTCTGACAAG 
AAGGTGGGCA TCTACGTCGA GGTGGACATG TTTGGCCTCC CAGTTGACAC AAGACGCAAA 
TATCGTACCC GGACATCCCA GGGGAACTCA TTCAACCCTG TGTGGGACGA GGAACCCTTT 
GACTTCCCCA AGGTTGTGCT GCCCACGCTG GCCTCACTTC GCATCGCAGC CTTTGAGGAG 
GGTGGCAAAT TTGTTGGGCA CCGTATCCTG CCTGTCTCTG CTATCCGCTC AGGATACCAC 
TATGTCTGCC TGCGAAACGA GGCCAACCAA CCTTTGTGCC TGCCTGCCCT GCTTATCTAC 
ACTGAAGCTT CTGACTACAT CCCAGATGAC CACCAGGACT ATGCGGAGGC CTTGATTAAC 
CCCATCAAGC ACGTAAGCCT AATGGACCAG CGGGCCAAGC AACTAGCCGC TCTCATTGGG 
GAGAGTGAGG CTCAGGCCAG CACAGAGACA TATCAGGAGA CCCCGTGTCA ACAGCCAGGG 
TCACAGCTCC CCTCCAACCC CACACCTAAC CCACTGGATG CCTCACCTCG CTGGCCCCCC 
GGTCCTACCA CTTCCTCCAC TAGCTCCTCC CTCAGTAGCC CAGGGCAGCG AGATGATTTG 
ATTGCCAGCA TCCTCTCTGA GGTGACCCCT ACACCTCTAG AGGAGCTCAG AAGCCACAAG 
GCTATGGTGA AGCTACGGAG CCGGCAGGAT CGAGACCTGC GGGAGTTGCA TAAGAAGCAC 
CAGCGCAAGG CTGTGGCCCT CACCCGCCGC CTTCTGGATG GCCTGGCCCA GGCCAGGGCT 
GAGGGGAAGT GCCGGCCCTC CCCCAGTGCC CTGGGCAAGG CCACCAACTC GGAGGACGTG 
AAGGAGGAAG AGGAGGCCAA ACAGTATCGA GAGTTCCAGA ACCGACAAGT ACAGAGCCTG 
TTGGAGCTGA GGGAAGCCCA GGCAGATGTG GAGACCAAGC GGAAGCTGGA GCATCTACGA 
CAGGCTCATC AGCGGCTCAA GGAGGTTGTC CTGGATACAC ACACAACACA GTTCAAGAGG 
CTGAAGGAGC TGAATGAAAG GGAGAAGAAG GAACTCCAGA AGATCCTGGA CAGGAAGCGC 
AACAACAGCA TCTCAGAGGC CAAGACAAGG GAGAAACACA AGAAGGAGGT GGAACTGACA 
GAGATTAATC GGCGGCACAT CACTGAGTCG GTAAACTCCA TCAGACGGCT GGAAGAGGCC 
CAGAAGCAGC GCCATGAACG CCTGGTGGCA GGGCAACAGC AAGTCCTCCA GCAGCTAGAG 
GAAGAGGAAC CCAAGCTGCT GGCCCAGCTG ACCCAGGAGT GTCAGGAACA GCGAGAGAGG 
TTGCCCCAGG AGATCCGTCG GTGCCTGCTG GGCGAGACAG CAGAGGGACT GGGGGACGGC 
CCCCTGGTGG CCTGTGCCAG CAATGGTCAT GCACCTGGGA GTGGTGGGCA CCTGTCCAGC 
GCTGACTCGG AGAGCCAGGA GGAGAACACC CAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526829.1
CDS	60..3758
Translation	MPGTPTSVLPDHLTPYSSLVALRKLESPVVLEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL

Target ORF information:

RefSeq Version	XM_006526766.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526766.4

ORF Insert Sequence:

ATGCCAGGGA CCCCCACTTC AGTGTTGCCT GACCACCTCA CCCCCTATTC CAGCCTTGTT 
GCACTCCGGA AACTTGAGTC CCCTGTTGTC CTGGAGGCCT CCAGTCGGAA CCTGGTGACC 
CTGCGTGTGG ACCCTAATGG CTTCTTCTTG TACTGGACAG GACCCAACAT GGAGGTGGAC 
ACACTGGACA TCAGCTCCAT CAGGGACACA AGGACAGGTC GTTATGCCCG CCTGCCCAAG 
GACCCTAAGA TTCGAGAAGT ACTGGGCTTT GGAGGTCCTG ACACCCGGCT GGAGGAGAAA 
CTGATGACAG TGGTGGCCGG GCCAGATCCA GTAAATACCA CATTCTTGAA CTTCATGGCC 
GTGCAAGATG ACACAGTCAA GGTCTGGTCA GAGGAGTTGT TTAAACTGGC CATGAACATA 
TTGGCTCAGA ACGCCTCCCG GAACACCTTC CTGCGGAAAG CATACACGAA GCTGAAGCTG 
CAGGTGAACC AGGATGGACG GATTCCGGTC AAGAACATTC TGAAGATGTT CTCGGCGGAC 
AAGAAGCGGG TGGAGACGGC GCTGGAGTCC TGTGGCCTCA ACTTCAACCG AAGTGAGTCC 
ATTCGCCCTG ATGAGTTTCC CTTGGAAATT TTTGAGCGGT TCTTGAATAA ACTGTGTCTG 
CGCCCGGATA TTGACAAGAT CCTGCTGGAG ATAGGTGCCA AGGGCAAGCC GTACCTCACT 
CTGGAGCAGC TCATGGACTT TATCAACCAG AAGCAGAGAG ACCCGAGACT CAACGAAGTG 
CTGTACCCGC CACTTCGGTC CTCACAGGCT CGGCTGCTCA TTGAGAAGTA CGAGACGAAC 
AAACAGTTCC TGGAGCGCGA CCAGATGTCT ATGGAGGGCT TCAGCCGCTA CCTGGGAGGG 
GAGGAGAATG GTATCCTGCC TCTGGAGGCC CTGGATCTGA GCATGGACAT GACCCAGCCA 
CTGAGCGCAT ATTTTATCAA CTCCTCACAC AACACCTATC TCACTGCGGG CCAGCTGGCT 
GGACCATCAT CGGTGGAGAT GTACCGCCAG GCACTGCTGT GGGGCTGCCG CTGTGTGGAG 
CTGGATGTAT GGAAGGGACG GCCACCGGAG GAAGAGCCTT TCATCACTCA TGGTTTCACC 
ATGACCACTG AGGTGCCATT GCGTGATGTG CTAGAGGCCA TCGCTGAGGC AGCCTTCAAG 
ACCTCGCCCT ACCCTGTCAT CCTCTCCTTT GAGAACCATG TCGACTCGGC AAAGCAGCAG 
GCCAAGATGG CTGAGTACTG CCGCTCTATC TTTGGGGATG CGCTGCTCAT TGACCCTCTC 
GACAAATACC CGCTATCTGC GGGCATCCCC CTGCCTAGTC CACAGGACCT GATGGGCCGT 
ATCCTGGTGA AGAATAAGAA GCGACATCGG CCCAGCACAG GTGTCCCTGA CAGCTCAGTG 
CGCAAGCGGC CTCTGGAACA GAGCAACTCG GCCTTGAGTG AGAGCTCGGC CGCCACAGAG 
CCCTCCTCAC CTCAGCTTGG GTCCCCCAGC TCCGACAGCT GCCCTGGCCT AAGCAATGGG 
GAGGAGGTGG GACTCGAGAA GACCAGCCTG GAGCCTCAGA AGTCTCTAGG TGAGGAGAGC 
CTCAGCCGGG AACCTAATGT GCCCATGCCT GACCGTGACC GAGAGGATGA GGAGGAAGAT 
GAAGAAGAAG AGGAAACAAC GGATCCAAAA AAGCCCACCA CTGATGAGGG CACAGCCAGC 
AGTGAGGTCA ATGCCACGGA GGAGATGTCA ACGCTCGTCA ACTACGTTGA GCCCGTCAAG 
TTCAAGTCCT TCGAGGCTGC TCGAAAAAGG AACAAGTGCT TCGAGATGTC ATCCTTCGTG 
GAGACCAAGG CAATGGAGCA ACTGACCAAG AGTCCTATGG AGTTTGTGGA ATACAACAAA 
CAGCAGCTCA GCCGCATCTA CCCTAAGGGC ACACGAGTGG ACTCCTCCAA CTACATGCCT 
CAGCTCTTCT GGAACGTGGG TTGCCAGCTT GTTGCCCTCA ACTTCCAGAC CTTGGATTTG 
CCAATGCAGC TCAACGCAGG CGTGTTTGAG TACAATGGGC GCAGCGGGTA CTTGCTCAAG 
CCCGAGTTTA TGCGGCGGCC GGACAAGTCT TTTGATCCCT TCACTGAGGT CATCGTAGAT 
GGCATAGTGG CCAACGCCTT GCGGGTCAAG GTGATTTCGG GGCAGTTCCT GTCTGACAAG 
AAGGTGGGCA TCTACGTCGA GGTGGACATG TTTGGCCTCC CAGTTGACAC AAGACGCAAA 
TATCGTACCC GGACATCCCA GGGGAACTCA TTCAACCCTG TGTGGGACGA GGAACCCTTT 
GACTTCCCCA AGGTTGTGCT GCCCACGCTG GCCTCACTTC GCATCGCAGC CTTTGAGGAG 
GGTGGCAAAT TTGTTGGGCA CCGTATCCTG CCTGTCTCTG CTATCCGCTC AGGATACCAC 
TATGTCTGCC TGCGAAACGA GGCCAACCAA CCTTTGTGCC TGCCTGCCCT GCTTATCTAC 
ACTGAAGCTT CTGACTACAT CCCAGATGAC CACCAGGACT ATGCGGAGGC CTTGATTAAC 
CCCATCAAGC ACGTAAGCCT AATGGACCAG CGGGCCAAGC AACTAGCCGC TCTCATTGGG 
GAGAGTGAGG CTCAGGCCAG CACAGAGACA TATCAGGAGA CCCCGTGTCA ACAGCCAGGG 
TCACAGCTCC CCTCCAACCC CACACCTAAC CCACTGGATG CCTCACCTCG CTGGCCCCCC 
GGTCCTACCA CTTCCTCCAC TAGCTCCTCC CTCAGTAGCC CAGGGCAGCG AGATGATTTG 
ATTGCCAGCA TCCTCTCTGA GGTGACCCCT ACACCTCTAG AGGAGCTCAG AAGCCACAAG 
GCTATGGTGA AGCTACGGAG CCGGCAGGAT CGAGACCTGC GGGAGTTGCA TAAGAAGCAC 
CAGCGCAAGG CTGTGGCCCT CACCCGCCGC CTTCTGGATG GCCTGGCCCA GGCCAGGGCT 
GAGGGGAAGT GCCGGCCCTC CCCCAGTGCC CTGGGCAAGG CCACCAACTC GGAGGACGTG 
AAGGAGGAAG AGGAGGCCAA ACAGTATCGA GAGTTCCAGA ACCGACAAGT ACAGAGCCTG 
TTGGAGCTGA GGGAAGCCCA GGCAGATGTG GAGACCAAGC GGAAGCTGGA GCATCTACGA 
CAGGCTCATC AGCGGCTCAA GGAGGTTGTC CTGGATACAC ACACAACACA GTTCAAGAGG 
CTGAAGGAGC TGAATGAAAG GGAGAAGAAG GAACTCCAGA AGATCCTGGA CAGGAAGCGC 
AACAACAGCA TCTCAGAGGC CAAGACAAGG GAGAAACACA AGAAGGAGGT GGAACTGACA 
GAGATTAATC GGCGGCACAT CACTGAGTCG GTAAACTCCA TCAGACGGCT GGAAGAGGCC 
CAGAAGCAGC GCCATGAACG CCTGGTGGCA GGGCAACAGC AAGTCCTCCA GCAGCTAGAG 
GAAGAGGAAC CCAAGCTGCT GGCCCAGCTG ACCCAGGAGT GTCAGGAACA GCGAGAGAGG 
TTGCCCCAGG AGATCCGTCG GTGCCTGCTG GGCGAGACAG CAGAGGGACT GGGGGACGGC 
CCCCTGGTGG CCTGTGCCAG CAATGGTCAT GCACCTGGGA GTGGTGGGCA CCTGTCCAGC 
GCTGACTCGG AGAGCCAGGA GGAGAACACC CAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb3 ( XM_006526767.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526830.1 Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu55310
Clone ID Related Accession (Same CDS sequence)	XM_006526767.4 , XM_006526767.3
Accession Version	XM_006526767.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526767.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAGG AGGCCTCCAG TCGGAACCTG GTGACCCTGC GTGTGGACCC TAATGGCTTC 
TTCTTGTACT GGACAGGACC CAACATGGAG GTGGACACAC TGGACATCAG CTCCATCAGG 
GACACAAGGA CAGGTCGTTA TGCCCGCCTG CCCAAGGACC CTAAGATTCG AGAAGTACTG 
GGCTTTGGAG GTCCTGACAC CCGGCTGGAG GAGAAACTGA TGACAGTGGT GGCCGGGCCA 
GATCCAGTAA ATACCACATT CTTGAACTTC ATGGCCGTGC AAGATGACAC AGTCAAGGTC 
TGGTCAGAGG AGTTGTTTAA ACTGGCCATG AACATATTGG CTCAGAACGC CTCCCGGAAC 
ACCTTCCTGC GGAAAGCATA CACGAAGCTG AAGCTGCAGG TGAACCAGGA TGGACGGATT 
CCGGTCAAGA ACATTCTGAA GATGTTCTCG GCGGACAAGA AGCGGGTGGA GACGGCGCTG 
GAGTCCTGTG GCCTCAACTT CAACCGAAGT GAGTCCATTC GCCCTGATGA GTTTCCCTTG 
GAAATTTTTG AGCGGTTCTT GAATAAACTG TGTCTGCGCC CGGATATTGA CAAGATCCTG 
CTGGAGATAG GTGCCAAGGG CAAGCCGTAC CTCACTCTGG AGCAGCTCAT GGACTTTATC 
AACCAGAAGC AGAGAGACCC GAGACTCAAC GAAGTGCTGT ACCCGCCACT TCGGTCCTCA 
CAGGCTCGGC TGCTCATTGA GAAGTACGAG ACGAACAAAC AGTTCCTGGA GCGCGACCAG 
ATGTCTATGG AGGGCTTCAG CCGCTACCTG GGAGGGGAGG AGAATGGTAT CCTGCCTCTG 
GAGGCCCTGG ATCTGAGCAT GGACATGACC CAGCCACTGA GCGCATATTT TATCAACTCC 
TCACACAACA CCTATCTCAC TGCGGGCCAG CTGGCTGGAC CATCATCGGT GGAGATGTAC 
CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT GTGGAGCTGG ATGTATGGAA GGGACGGCCA 
CCGGAGGAAG AGCCTTTCAT CACTCATGGT TTCACCATGA CCACTGAGGT GCCATTGCGT 
GATGTGCTAG AGGCCATCGC TGAGGCAGCC TTCAAGACCT CGCCCTACCC TGTCATCCTC 
TCCTTTGAGA ACCATGTCGA CTCGGCAAAG CAGCAGGCCA AGATGGCTGA GTACTGCCGC 
TCTATCTTTG GGGATGCGCT GCTCATTGAC CCTCTCGACA AATACCCGCT ATCTGCGGGC 
ATCCCCCTGC CTAGTCCACA GGACCTGATG GGCCGTATCC TGGTGAAGAA TAAGAAGCGA 
CATCGGCCCA GCACAGGTGT CCCTGACAGC TCAGTGCGCA AGCGGCCTCT GGAACAGAGC 
AACTCGGCCT TGAGTGAGAG CTCGGCCGCC ACAGAGCCCT CCTCACCTCA GCTTGGGTCC 
CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC AATGGGGAGG AGGTGGGACT CGAGAAGACC 
AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG GAGAGCCTCA GCCGGGAACC TAATGTGCCC 
ATGCCTGACC GTGACCGAGA GGATGAGGAG GAAGATGAAG AAGAAGAGGA AACAACGGAT 
CCAAAAAAGC CCACCACTGA TGAGGGCACA GCCAGCAGTG AGGTCAATGC CACGGAGGAG 
ATGTCAACGC TCGTCAACTA CGTTGAGCCC GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA 
AAAAGGAACA AGTGCTTCGA GATGTCATCC TTCGTGGAGA CCAAGGCAAT GGAGCAACTG 
ACCAAGAGTC CTATGGAGTT TGTGGAATAC AACAAACAGC AGCTCAGCCG CATCTACCCT 
AAGGGCACAC GAGTGGACTC CTCCAACTAC ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC 
CAGCTTGTTG CCCTCAACTT CCAGACCTTG GATTTGCCAA TGCAGCTCAA CGCAGGCGTG 
TTTGAGTACA ATGGGCGCAG CGGGTACTTG CTCAAGCCCG AGTTTATGCG GCGGCCGGAC 
AAGTCTTTTG ATCCCTTCAC TGAGGTCATC GTAGATGGCA TAGTGGCCAA CGCCTTGCGG 
GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT GACAAGAAGG TGGGCATCTA CGTCGAGGTG 
GACATGTTTG GCCTCCCAGT TGACACAAGA CGCAAATATC GTACCCGGAC ATCCCAGGGG 
AACTCATTCA ACCCTGTGTG GGACGAGGAA CCCTTTGACT TCCCCAAGGT TGTGCTGCCC 
ACGCTGGCCT CACTTCGCAT CGCAGCCTTT GAGGAGGGTG GCAAATTTGT TGGGCACCGT 
ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA TACCACTATG TCTGCCTGCG AAACGAGGCC 
AACCAACCTT TGTGCCTGCC TGCCCTGCTT ATCTACACTG AAGCTTCTGA CTACATCCCA 
GATGACCACC AGGACTATGC GGAGGCCTTG ATTAACCCCA TCAAGCACGT AAGCCTAATG 
GACCAGCGGG CCAAGCAACT AGCCGCTCTC ATTGGGGAGA GTGAGGCTCA GGCCAGCACA 
GAGACATATC AGGAGACCCC GTGTCAACAG CCAGGGTCAC AGCTCCCCTC CAACCCCACA 
CCTAACCCAC TGGATGCCTC ACCTCGCTGG CCCCCCGGTC CTACCACTTC CTCCACTAGC 
TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT GATTTGATTG CCAGCATCCT CTCTGAGGTG 
ACCCCTACAC CTCTAGAGGA GCTCAGAAGC CACAAGGCTA TGGTGAAGCT ACGGAGCCGG 
CAGGATCGAG ACCTGCGGGA GTTGCATAAG AAGCACCAGC GCAAGGCTGT GGCCCTCACC 
CGCCGCCTTC TGGATGGCCT GGCCCAGGCC AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC 
AGTGCCCTGG GCAAGGCCAC CAACTCGGAG GACGTGAAGG AGGAAGAGGA GGCCAAACAG 
TATCGAGAGT TCCAGAACCG ACAAGTACAG AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA 
GATGTGGAGA CCAAGCGGAA GCTGGAGCAT CTACGACAGG CTCATCAGCG GCTCAAGGAG 
GTTGTCCTGG ATACACACAC AACACAGTTC AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG 
AAGAAGGAAC TCCAGAAGAT CCTGGACAGG AAGCGCAACA ACAGCATCTC AGAGGCCAAG 
ACAAGGGAGA AACACAAGAA GGAGGTGGAA CTGACAGAGA TTAATCGGCG GCACATCACT 
GAGTCGGTAA ACTCCATCAG ACGGCTGGAA GAGGCCCAGA AGCAGCGCCA TGAACGCCTG 
GTGGCAGGGC AACAGCAAGT CCTCCAGCAG CTAGAGGAAG AGGAACCCAA GCTGCTGGCC 
CAGCTGACCC AGGAGTGTCA GGAACAGCGA GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC 
CTGCTGGGCG AGACAGCAGA GGGACTGGGG GACGGCCCCC TGGTGGCCTG TGCCAGCAAT 
GGTCATGCAC CTGGGAGTGG TGGGCACCTG TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG 
AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526830.1
CDS	804..4418
Translation	MIKEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL

Target ORF information:

RefSeq Version	XM_006526767.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526767.4

ORF Insert Sequence:

ATGATAAAGG AGGCCTCCAG TCGGAACCTG GTGACCCTGC GTGTGGACCC TAATGGCTTC 
TTCTTGTACT GGACAGGACC CAACATGGAG GTGGACACAC TGGACATCAG CTCCATCAGG 
GACACAAGGA CAGGTCGTTA TGCCCGCCTG CCCAAGGACC CTAAGATTCG AGAAGTACTG 
GGCTTTGGAG GTCCTGACAC CCGGCTGGAG GAGAAACTGA TGACAGTGGT GGCCGGGCCA 
GATCCAGTAA ATACCACATT CTTGAACTTC ATGGCCGTGC AAGATGACAC AGTCAAGGTC 
TGGTCAGAGG AGTTGTTTAA ACTGGCCATG AACATATTGG CTCAGAACGC CTCCCGGAAC 
ACCTTCCTGC GGAAAGCATA CACGAAGCTG AAGCTGCAGG TGAACCAGGA TGGACGGATT 
CCGGTCAAGA ACATTCTGAA GATGTTCTCG GCGGACAAGA AGCGGGTGGA GACGGCGCTG 
GAGTCCTGTG GCCTCAACTT CAACCGAAGT GAGTCCATTC GCCCTGATGA GTTTCCCTTG 
GAAATTTTTG AGCGGTTCTT GAATAAACTG TGTCTGCGCC CGGATATTGA CAAGATCCTG 
CTGGAGATAG GTGCCAAGGG CAAGCCGTAC CTCACTCTGG AGCAGCTCAT GGACTTTATC 
AACCAGAAGC AGAGAGACCC GAGACTCAAC GAAGTGCTGT ACCCGCCACT TCGGTCCTCA 
CAGGCTCGGC TGCTCATTGA GAAGTACGAG ACGAACAAAC AGTTCCTGGA GCGCGACCAG 
ATGTCTATGG AGGGCTTCAG CCGCTACCTG GGAGGGGAGG AGAATGGTAT CCTGCCTCTG 
GAGGCCCTGG ATCTGAGCAT GGACATGACC CAGCCACTGA GCGCATATTT TATCAACTCC 
TCACACAACA CCTATCTCAC TGCGGGCCAG CTGGCTGGAC CATCATCGGT GGAGATGTAC 
CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT GTGGAGCTGG ATGTATGGAA GGGACGGCCA 
CCGGAGGAAG AGCCTTTCAT CACTCATGGT TTCACCATGA CCACTGAGGT GCCATTGCGT 
GATGTGCTAG AGGCCATCGC TGAGGCAGCC TTCAAGACCT CGCCCTACCC TGTCATCCTC 
TCCTTTGAGA ACCATGTCGA CTCGGCAAAG CAGCAGGCCA AGATGGCTGA GTACTGCCGC 
TCTATCTTTG GGGATGCGCT GCTCATTGAC CCTCTCGACA AATACCCGCT ATCTGCGGGC 
ATCCCCCTGC CTAGTCCACA GGACCTGATG GGCCGTATCC TGGTGAAGAA TAAGAAGCGA 
CATCGGCCCA GCACAGGTGT CCCTGACAGC TCAGTGCGCA AGCGGCCTCT GGAACAGAGC 
AACTCGGCCT TGAGTGAGAG CTCGGCCGCC ACAGAGCCCT CCTCACCTCA GCTTGGGTCC 
CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC AATGGGGAGG AGGTGGGACT CGAGAAGACC 
AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG GAGAGCCTCA GCCGGGAACC TAATGTGCCC 
ATGCCTGACC GTGACCGAGA GGATGAGGAG GAAGATGAAG AAGAAGAGGA AACAACGGAT 
CCAAAAAAGC CCACCACTGA TGAGGGCACA GCCAGCAGTG AGGTCAATGC CACGGAGGAG 
ATGTCAACGC TCGTCAACTA CGTTGAGCCC GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA 
AAAAGGAACA AGTGCTTCGA GATGTCATCC TTCGTGGAGA CCAAGGCAAT GGAGCAACTG 
ACCAAGAGTC CTATGGAGTT TGTGGAATAC AACAAACAGC AGCTCAGCCG CATCTACCCT 
AAGGGCACAC GAGTGGACTC CTCCAACTAC ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC 
CAGCTTGTTG CCCTCAACTT CCAGACCTTG GATTTGCCAA TGCAGCTCAA CGCAGGCGTG 
TTTGAGTACA ATGGGCGCAG CGGGTACTTG CTCAAGCCCG AGTTTATGCG GCGGCCGGAC 
AAGTCTTTTG ATCCCTTCAC TGAGGTCATC GTAGATGGCA TAGTGGCCAA CGCCTTGCGG 
GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT GACAAGAAGG TGGGCATCTA CGTCGAGGTG 
GACATGTTTG GCCTCCCAGT TGACACAAGA CGCAAATATC GTACCCGGAC ATCCCAGGGG 
AACTCATTCA ACCCTGTGTG GGACGAGGAA CCCTTTGACT TCCCCAAGGT TGTGCTGCCC 
ACGCTGGCCT CACTTCGCAT CGCAGCCTTT GAGGAGGGTG GCAAATTTGT TGGGCACCGT 
ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA TACCACTATG TCTGCCTGCG AAACGAGGCC 
AACCAACCTT TGTGCCTGCC TGCCCTGCTT ATCTACACTG AAGCTTCTGA CTACATCCCA 
GATGACCACC AGGACTATGC GGAGGCCTTG ATTAACCCCA TCAAGCACGT AAGCCTAATG 
GACCAGCGGG CCAAGCAACT AGCCGCTCTC ATTGGGGAGA GTGAGGCTCA GGCCAGCACA 
GAGACATATC AGGAGACCCC GTGTCAACAG CCAGGGTCAC AGCTCCCCTC CAACCCCACA 
CCTAACCCAC TGGATGCCTC ACCTCGCTGG CCCCCCGGTC CTACCACTTC CTCCACTAGC 
TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT GATTTGATTG CCAGCATCCT CTCTGAGGTG 
ACCCCTACAC CTCTAGAGGA GCTCAGAAGC CACAAGGCTA TGGTGAAGCT ACGGAGCCGG 
CAGGATCGAG ACCTGCGGGA GTTGCATAAG AAGCACCAGC GCAAGGCTGT GGCCCTCACC 
CGCCGCCTTC TGGATGGCCT GGCCCAGGCC AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC 
AGTGCCCTGG GCAAGGCCAC CAACTCGGAG GACGTGAAGG AGGAAGAGGA GGCCAAACAG 
TATCGAGAGT TCCAGAACCG ACAAGTACAG AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA 
GATGTGGAGA CCAAGCGGAA GCTGGAGCAT CTACGACAGG CTCATCAGCG GCTCAAGGAG 
GTTGTCCTGG ATACACACAC AACACAGTTC AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG 
AAGAAGGAAC TCCAGAAGAT CCTGGACAGG AAGCGCAACA ACAGCATCTC AGAGGCCAAG 
ACAAGGGAGA AACACAAGAA GGAGGTGGAA CTGACAGAGA TTAATCGGCG GCACATCACT 
GAGTCGGTAA ACTCCATCAG ACGGCTGGAA GAGGCCCAGA AGCAGCGCCA TGAACGCCTG 
GTGGCAGGGC AACAGCAAGT CCTCCAGCAG CTAGAGGAAG AGGAACCCAA GCTGCTGGCC 
CAGCTGACCC AGGAGTGTCA GGAACAGCGA GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC 
CTGCTGGGCG AGACAGCAGA GGGACTGGGG GACGGCCCCC TGGTGGCCTG TGCCAGCAAT 
GGTCATGCAC CTGGGAGTGG TGGGCACCTG TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG 
AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb3 ( NM_008874.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032900.2 Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant 2, mRNA.

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CloneID	OMu13272
Clone ID Related Accession (Same CDS sequence)	NM_008874.4
Accession Version	NM_008874.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC120557.12, AK146793.1, CB527492.1 and AI851116.1. On Mar 12, 2014 this sequence version replaced NM_008874.3. Transcript Variant: This variant (2) uses two alternate splice sites that result in an alternate codon in the 5' coding region, compared to variant 1. The encoded isoform (b) is the same length as isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035928.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG CGAGGCCCGG CGTCCACGCG CTGCAGCTGG AGCCGCCCAC CGTGGTGGAG 
ACCCTGCGGC GCGGGAGTAA GTTCATCAAA TGGGACGAGG AGGCCTCCAG TCGGAACCTG 
GTGACCCTGC GTGTGGACCC TAATGGCTTC TTCTTGTACT GGACAGGACC CAACATGGAG 
GTGGACACAC TGGACATCAG CTCCATCAGG GACACAAGGA CAGGTCGTTA TGCCCGCCTG 
CCCAAGGACC CTAAGATTCG AGAAGTACTG GGCTTTGGAG GTCCTGACAC CCGGCTGGAG 
GAGAAACTGA TGACAGTGGT GGCCGGGCCA GATCCAGTAA ATACCACATT CTTGAACTTC 
ATGGCCGTGC AAGATGACAC AGTCAAGGTC TGGTCAGAGG AGTTGTTTAA ACTGGCCATG 
AACATATTGG CTCAGAACGC CTCCCGGAAC ACCTTCCTGC GGAAAGCATA CACGAAGCTG 
AAGCTGCAGG TGAACCAGGA TGGACGGATT CCGGTCAAGA ACATTCTGAA GATGTTCTCG 
GCGGACAAGA AGCGGGTGGA GACGGCGCTG GAGTCCTGTG GCCTCAACTT CAACCGAGTT 
GAGTCCATTC GCCCTGATGA GTTTCCCTTG GAAATTTTTG AGCGGTTCTT GAATAAACTG 
TGTCTGCGCC CGGATATTGA CAAGATCCTG CTGGAGATAG GTGCCAAGGG CAAGCCGTAC 
CTCACTCTGG AGCAGCTCAT GGACTTTATC AACCAGAAGC AGAGAGACCC GAGACTCAAC 
GAAGTGCTGT ACCCGCCACT TCGGTCCTCA CAGGCTCGGC TGCTCATTGA GAAGTACGAG 
ACGAACAAAC AGTTCCTGGA GCGCGACCAG ATGTCTATGG AGGGCTTCAG CCGCTACCTG 
GGAGGGGAGG AGAATGGTAT CCTGCCTCTG GAGGCCCTGG ATCTGAGCAT GGACATGACC 
CAGCCACTGA GCGCATATTT TATCAACTCC TCACACAACA CCTATCTCAC TGCGGGCCAG 
CTGGCTGGAC CATCATCGGT GGAGATGTAC CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT 
GTGGAGCTGG ATGTATGGAA GGGACGGCCA CCGGAGGAAG AGCCTTTCAT CACTCATGGT 
TTCACCATGA CCACTGAGGT GCCATTGCGT GATGTGCTAG AGGCCATCGC TGAGGCAGCC 
TTCAAGACCT CGCCCTACCC TGTCATCCTC TCCTTTGAGA ACCATGTCGA CTCGGCAAAG 
CAGCAGGCCA AGATGGCTGA GTACTGCCGC TCTATCTTTG GGGATGCGCT GCTCATTGAC 
CCTCTCGACA AATACCCGCT ATCTGCGGGC ATCCCCCTGC CTAGTCCACA GGACCTGATG 
GGCCGTATCC TGGTGAAGAA TAAGAAGCGA CATCGGCCCA GCACAGGTGT CCCTGACAGC 
TCAGTGCGCA AGCGGCCTCT GGAACAGAGC AACTCGGCCT TGAGTGAGAG CTCGGCCGCC 
ACAGAGCCCT CCTCACCTCA GCTTGGGTCC CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC 
AATGGGGAGG AGGTGGGACT CGAGAAGACC AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG 
GAGAGCCTCA GCCGGGAACC TAATGTGCCC ATGCCTGACC GTGACCGAGA GGATGAGGAG 
GAAGATGAAG AAGAAGAGGA AACAACGGAT CCAAAAAAGC CCACCACTGA TGAGGGCACA 
GCCAGCAGTG AGGTCAATGC CACGGAGGAG ATGTCAACGC TCGTCAACTA CGTTGAGCCC 
GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA AAAAGGAACA AGTGCTTCGA GATGTCATCC 
TTCGTGGAGA CCAAGGCAAT GGAGCAACTG ACCAAGAGTC CTATGGAGTT TGTGGAATAC 
AACAAACAGC AGCTCAGCCG CATCTACCCT AAGGGCACAC GAGTGGACTC CTCCAACTAC 
ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC CAGCTTGTTG CCCTCAACTT CCAGACCTTG 
GATTTGCCAA TGCAGCTCAA CGCAGGCGTG TTTGAGTACA ATGGGCGCAG CGGGTACTTG 
CTCAAGCCCG AGTTTATGCG GCGGCCGGAC AAGTCTTTTG ATCCCTTCAC TGAGGTCATC 
GTAGATGGCA TAGTGGCCAA CGCCTTGCGG GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT 
GACAAGAAGG TGGGCATCTA CGTCGAGGTG GACATGTTTG GCCTCCCAGT TGACACAAGA 
CGCAAATATC GTACCCGGAC ATCCCAGGGG AACTCATTCA ACCCTGTGTG GGACGAGGAA 
CCCTTTGACT TCCCCAAGGT TGTGCTGCCC ACGCTGGCCT CACTTCGCAT CGCAGCCTTT 
GAGGAGGGTG GCAAATTTGT TGGGCACCGT ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA 
TACCACTATG TCTGCCTGCG AAACGAGGCC AACCAACCTT TGTGCCTGCC TGCCCTGCTT 
ATCTACACTG AAGCTTCTGA CTACATCCCA GATGACCACC AGGACTATGC GGAGGCCTTG 
ATTAACCCCA TCAAGCACGT AAGCCTAATG GACCAGCGGG CCAAGCAACT AGCCGCTCTC 
ATTGGGGAGA GTGAGGCTCA GGCCAGCACA GAGACATATC AGGAGACCCC GTGTCAACAG 
CCAGGGTCAC AGCTCCCCTC CAACCCCACA CCTAACCCAC TGGATGCCTC ACCTCGCTGG 
CCCCCCGGTC CTACCACTTC CTCCACTAGC TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT 
GATTTGATTG CCAGCATCCT CTCTGAGGTG ACCCCTACAC CTCTAGAGGA GCTCAGAAGC 
CACAAGGCTA TGGTGAAGCT ACGGAGCCGG CAGGATCGAG ACCTGCGGGA GTTGCATAAG 
AAGCACCAGC GCAAGGCTGT GGCCCTCACC CGCCGCCTTC TGGATGGCCT GGCCCAGGCC 
AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC AGTGCCCTGG GCAAGGCCAC CAACTCGGAG 
GACGTGAAGG AGGAAGAGGA GGCCAAACAG TATCGAGAGT TCCAGAACCG ACAAGTACAG 
AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA GATGTGGAGA CCAAGCGGAA GCTGGAGCAT 
CTACGACAGG CTCATCAGCG GCTCAAGGAG GTTGTCCTGG ATACACACAC AACACAGTTC 
AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG AAGAAGGAAC TCCAGAAGAT CCTGGACAGG 
AAGCGCAACA ACAGCATCTC AGAGGCCAAG ACAAGGGAGA AACACAAGAA GGAGGTGGAA 
CTGACAGAGA TTAATCGGCG GCACATCACT GAGTCGGTAA ACTCCATCAG ACGGCTGGAA 
GAGGCCCAGA AGCAGCGCCA TGAACGCCTG GTGGCAGGGC AACAGCAAGT CCTCCAGCAG 
CTAGAGGAAG AGGAACCCAA GCTGCTGGCC CAGCTGACCC AGGAGTGTCA GGAACAGCGA 
GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC CTGCTGGGCG AGACAGCAGA GGGACTGGGG 
GACGGCCCCC TGGTGGCCTG TGCCAGCAAT GGTCATGCAC CTGGGAGTGG TGGGCACCTG 
TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032900.2
CDS	170..3874
Translation	MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRVESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL

Target ORF information:

RefSeq Version	NM_008874.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008874.4

ORF Insert Sequence:

ATGGCGGGCG CGAGGCCCGG CGTCCACGCG CTGCAGCTGG AGCCGCCCAC CGTGGTGGAG 
ACCCTGCGGC GCGGGAGTAA GTTCATCAAA TGGGACGAGG AGGCCTCCAG TCGGAACCTG 
GTGACCCTGC GTGTGGACCC TAATGGCTTC TTCTTGTACT GGACAGGACC CAACATGGAG 
GTGGACACAC TGGACATCAG CTCCATCAGG GACACAAGGA CAGGTCGTTA TGCCCGCCTG 
CCCAAGGACC CTAAGATTCG AGAAGTACTG GGCTTTGGAG GTCCTGACAC CCGGCTGGAG 
GAGAAACTGA TGACAGTGGT GGCCGGGCCA GATCCAGTAA ATACCACATT CTTGAACTTC 
ATGGCCGTGC AAGATGACAC AGTCAAGGTC TGGTCAGAGG AGTTGTTTAA ACTGGCCATG 
AACATATTGG CTCAGAACGC CTCCCGGAAC ACCTTCCTGC GGAAAGCATA CACGAAGCTG 
AAGCTGCAGG TGAACCAGGA TGGACGGATT CCGGTCAAGA ACATTCTGAA GATGTTCTCG 
GCGGACAAGA AGCGGGTGGA GACGGCGCTG GAGTCCTGTG GCCTCAACTT CAACCGAGTT 
GAGTCCATTC GCCCTGATGA GTTTCCCTTG GAAATTTTTG AGCGGTTCTT GAATAAACTG 
TGTCTGCGCC CGGATATTGA CAAGATCCTG CTGGAGATAG GTGCCAAGGG CAAGCCGTAC 
CTCACTCTGG AGCAGCTCAT GGACTTTATC AACCAGAAGC AGAGAGACCC GAGACTCAAC 
GAAGTGCTGT ACCCGCCACT TCGGTCCTCA CAGGCTCGGC TGCTCATTGA GAAGTACGAG 
ACGAACAAAC AGTTCCTGGA GCGCGACCAG ATGTCTATGG AGGGCTTCAG CCGCTACCTG 
GGAGGGGAGG AGAATGGTAT CCTGCCTCTG GAGGCCCTGG ATCTGAGCAT GGACATGACC 
CAGCCACTGA GCGCATATTT TATCAACTCC TCACACAACA CCTATCTCAC TGCGGGCCAG 
CTGGCTGGAC CATCATCGGT GGAGATGTAC CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT 
GTGGAGCTGG ATGTATGGAA GGGACGGCCA CCGGAGGAAG AGCCTTTCAT CACTCATGGT 
TTCACCATGA CCACTGAGGT GCCATTGCGT GATGTGCTAG AGGCCATCGC TGAGGCAGCC 
TTCAAGACCT CGCCCTACCC TGTCATCCTC TCCTTTGAGA ACCATGTCGA CTCGGCAAAG 
CAGCAGGCCA AGATGGCTGA GTACTGCCGC TCTATCTTTG GGGATGCGCT GCTCATTGAC 
CCTCTCGACA AATACCCGCT ATCTGCGGGC ATCCCCCTGC CTAGTCCACA GGACCTGATG 
GGCCGTATCC TGGTGAAGAA TAAGAAGCGA CATCGGCCCA GCACAGGTGT CCCTGACAGC 
TCAGTGCGCA AGCGGCCTCT GGAACAGAGC AACTCGGCCT TGAGTGAGAG CTCGGCCGCC 
ACAGAGCCCT CCTCACCTCA GCTTGGGTCC CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC 
AATGGGGAGG AGGTGGGACT CGAGAAGACC AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG 
GAGAGCCTCA GCCGGGAACC TAATGTGCCC ATGCCTGACC GTGACCGAGA GGATGAGGAG 
GAAGATGAAG AAGAAGAGGA AACAACGGAT CCAAAAAAGC CCACCACTGA TGAGGGCACA 
GCCAGCAGTG AGGTCAATGC CACGGAGGAG ATGTCAACGC TCGTCAACTA CGTTGAGCCC 
GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA AAAAGGAACA AGTGCTTCGA GATGTCATCC 
TTCGTGGAGA CCAAGGCAAT GGAGCAACTG ACCAAGAGTC CTATGGAGTT TGTGGAATAC 
AACAAACAGC AGCTCAGCCG CATCTACCCT AAGGGCACAC GAGTGGACTC CTCCAACTAC 
ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC CAGCTTGTTG CCCTCAACTT CCAGACCTTG 
GATTTGCCAA TGCAGCTCAA CGCAGGCGTG TTTGAGTACA ATGGGCGCAG CGGGTACTTG 
CTCAAGCCCG AGTTTATGCG GCGGCCGGAC AAGTCTTTTG ATCCCTTCAC TGAGGTCATC 
GTAGATGGCA TAGTGGCCAA CGCCTTGCGG GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT 
GACAAGAAGG TGGGCATCTA CGTCGAGGTG GACATGTTTG GCCTCCCAGT TGACACAAGA 
CGCAAATATC GTACCCGGAC ATCCCAGGGG AACTCATTCA ACCCTGTGTG GGACGAGGAA 
CCCTTTGACT TCCCCAAGGT TGTGCTGCCC ACGCTGGCCT CACTTCGCAT CGCAGCCTTT 
GAGGAGGGTG GCAAATTTGT TGGGCACCGT ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA 
TACCACTATG TCTGCCTGCG AAACGAGGCC AACCAACCTT TGTGCCTGCC TGCCCTGCTT 
ATCTACACTG AAGCTTCTGA CTACATCCCA GATGACCACC AGGACTATGC GGAGGCCTTG 
ATTAACCCCA TCAAGCACGT AAGCCTAATG GACCAGCGGG CCAAGCAACT AGCCGCTCTC 
ATTGGGGAGA GTGAGGCTCA GGCCAGCACA GAGACATATC AGGAGACCCC GTGTCAACAG 
CCAGGGTCAC AGCTCCCCTC CAACCCCACA CCTAACCCAC TGGATGCCTC ACCTCGCTGG 
CCCCCCGGTC CTACCACTTC CTCCACTAGC TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT 
GATTTGATTG CCAGCATCCT CTCTGAGGTG ACCCCTACAC CTCTAGAGGA GCTCAGAAGC 
CACAAGGCTA TGGTGAAGCT ACGGAGCCGG CAGGATCGAG ACCTGCGGGA GTTGCATAAG 
AAGCACCAGC GCAAGGCTGT GGCCCTCACC CGCCGCCTTC TGGATGGCCT GGCCCAGGCC 
AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC AGTGCCCTGG GCAAGGCCAC CAACTCGGAG 
GACGTGAAGG AGGAAGAGGA GGCCAAACAG TATCGAGAGT TCCAGAACCG ACAAGTACAG 
AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA GATGTGGAGA CCAAGCGGAA GCTGGAGCAT 
CTACGACAGG CTCATCAGCG GCTCAAGGAG GTTGTCCTGG ATACACACAC AACACAGTTC 
AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG AAGAAGGAAC TCCAGAAGAT CCTGGACAGG 
AAGCGCAACA ACAGCATCTC AGAGGCCAAG ACAAGGGAGA AACACAAGAA GGAGGTGGAA 
CTGACAGAGA TTAATCGGCG GCACATCACT GAGTCGGTAA ACTCCATCAG ACGGCTGGAA 
GAGGCCCAGA AGCAGCGCCA TGAACGCCTG GTGGCAGGGC AACAGCAAGT CCTCCAGCAG 
CTAGAGGAAG AGGAACCCAA GCTGCTGGCC CAGCTGACCC AGGAGTGTCA GGAACAGCGA 
GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC CTGCTGGGCG AGACAGCAGA GGGACTGGGG 
GACGGCCCCC TGGTGGCCTG TGCCAGCAAT GGTCATGCAC CTGGGAGTGG TGGGCACCTG 
TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb3 ( NM_001290349.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277278.1 Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant 1, mRNA.

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CloneID	OMu17008
Clone ID Related Accession (Same CDS sequence)	NM_001290349.1
Accession Version	NM_001290349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC120557.12, AK146793.1 and AI851116.1. On Mar 12, 2014 this sequence version replaced XM_006526765.1. Transcript Variant: This variant (1) encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43144.1, AK146793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG CGAGGCCCGG CGTCCACGCG CTGCAGCTGG AGCCGCCCAC CGTGGTGGAG 
ACCCTGCGGC GCGGGAGTAA GTTCATCAAA TGGGACGAGG AGGCCTCCAG TCGGAACCTG 
GTGACCCTGC GTGTGGACCC TAATGGCTTC TTCTTGTACT GGACAGGACC CAACATGGAG 
GTGGACACAC TGGACATCAG CTCCATCAGG GACACAAGGA CAGGTCGTTA TGCCCGCCTG 
CCCAAGGACC CTAAGATTCG AGAAGTACTG GGCTTTGGAG GTCCTGACAC CCGGCTGGAG 
GAGAAACTGA TGACAGTGGT GGCCGGGCCA GATCCAGTAA ATACCACATT CTTGAACTTC 
ATGGCCGTGC AAGATGACAC AGTCAAGGTC TGGTCAGAGG AGTTGTTTAA ACTGGCCATG 
AACATATTGG CTCAGAACGC CTCCCGGAAC ACCTTCCTGC GGAAAGCATA CACGAAGCTG 
AAGCTGCAGG TGAACCAGGA TGGACGGATT CCGGTCAAGA ACATTCTGAA GATGTTCTCG 
GCGGACAAGA AGCGGGTGGA GACGGCGCTG GAGTCCTGTG GCCTCAACTT CAACCGAAGT 
GAGTCCATTC GCCCTGATGA GTTTCCCTTG GAAATTTTTG AGCGGTTCTT GAATAAACTG 
TGTCTGCGCC CGGATATTGA CAAGATCCTG CTGGAGATAG GTGCCAAGGG CAAGCCGTAC 
CTCACTCTGG AGCAGCTCAT GGACTTTATC AACCAGAAGC AGAGAGACCC GAGACTCAAC 
GAAGTGCTGT ACCCGCCACT TCGGTCCTCA CAGGCTCGGC TGCTCATTGA GAAGTACGAG 
ACGAACAAAC AGTTCCTGGA GCGCGACCAG ATGTCTATGG AGGGCTTCAG CCGCTACCTG 
GGAGGGGAGG AGAATGGTAT CCTGCCTCTG GAGGCCCTGG ATCTGAGCAT GGACATGACC 
CAGCCACTGA GCGCATATTT TATCAACTCC TCACACAACA CCTATCTCAC TGCGGGCCAG 
CTGGCTGGAC CATCATCGGT GGAGATGTAC CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT 
GTGGAGCTGG ATGTATGGAA GGGACGGCCA CCGGAGGAAG AGCCTTTCAT CACTCATGGT 
TTCACCATGA CCACTGAGGT GCCATTGCGT GATGTGCTAG AGGCCATCGC TGAGGCAGCC 
TTCAAGACCT CGCCCTACCC TGTCATCCTC TCCTTTGAGA ACCATGTCGA CTCGGCAAAG 
CAGCAGGCCA AGATGGCTGA GTACTGCCGC TCTATCTTTG GGGATGCGCT GCTCATTGAC 
CCTCTCGACA AATACCCGCT ATCTGCGGGC ATCCCCCTGC CTAGTCCACA GGACCTGATG 
GGCCGTATCC TGGTGAAGAA TAAGAAGCGA CATCGGCCCA GCACAGGTGT CCCTGACAGC 
TCAGTGCGCA AGCGGCCTCT GGAACAGAGC AACTCGGCCT TGAGTGAGAG CTCGGCCGCC 
ACAGAGCCCT CCTCACCTCA GCTTGGGTCC CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC 
AATGGGGAGG AGGTGGGACT CGAGAAGACC AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG 
GAGAGCCTCA GCCGGGAACC TAATGTGCCC ATGCCTGACC GTGACCGAGA GGATGAGGAG 
GAAGATGAAG AAGAAGAGGA AACAACGGAT CCAAAAAAGC CCACCACTGA TGAGGGCACA 
GCCAGCAGTG AGGTCAATGC CACGGAGGAG ATGTCAACGC TCGTCAACTA CGTTGAGCCC 
GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA AAAAGGAACA AGTGCTTCGA GATGTCATCC 
TTCGTGGAGA CCAAGGCAAT GGAGCAACTG ACCAAGAGTC CTATGGAGTT TGTGGAATAC 
AACAAACAGC AGCTCAGCCG CATCTACCCT AAGGGCACAC GAGTGGACTC CTCCAACTAC 
ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC CAGCTTGTTG CCCTCAACTT CCAGACCTTG 
GATTTGCCAA TGCAGCTCAA CGCAGGCGTG TTTGAGTACA ATGGGCGCAG CGGGTACTTG 
CTCAAGCCCG AGTTTATGCG GCGGCCGGAC AAGTCTTTTG ATCCCTTCAC TGAGGTCATC 
GTAGATGGCA TAGTGGCCAA CGCCTTGCGG GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT 
GACAAGAAGG TGGGCATCTA CGTCGAGGTG GACATGTTTG GCCTCCCAGT TGACACAAGA 
CGCAAATATC GTACCCGGAC ATCCCAGGGG AACTCATTCA ACCCTGTGTG GGACGAGGAA 
CCCTTTGACT TCCCCAAGGT TGTGCTGCCC ACGCTGGCCT CACTTCGCAT CGCAGCCTTT 
GAGGAGGGTG GCAAATTTGT TGGGCACCGT ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA 
TACCACTATG TCTGCCTGCG AAACGAGGCC AACCAACCTT TGTGCCTGCC TGCCCTGCTT 
ATCTACACTG AAGCTTCTGA CTACATCCCA GATGACCACC AGGACTATGC GGAGGCCTTG 
ATTAACCCCA TCAAGCACGT AAGCCTAATG GACCAGCGGG CCAAGCAACT AGCCGCTCTC 
ATTGGGGAGA GTGAGGCTCA GGCCAGCACA GAGACATATC AGGAGACCCC GTGTCAACAG 
CCAGGGTCAC AGCTCCCCTC CAACCCCACA CCTAACCCAC TGGATGCCTC ACCTCGCTGG 
CCCCCCGGTC CTACCACTTC CTCCACTAGC TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT 
GATTTGATTG CCAGCATCCT CTCTGAGGTG ACCCCTACAC CTCTAGAGGA GCTCAGAAGC 
CACAAGGCTA TGGTGAAGCT ACGGAGCCGG CAGGATCGAG ACCTGCGGGA GTTGCATAAG 
AAGCACCAGC GCAAGGCTGT GGCCCTCACC CGCCGCCTTC TGGATGGCCT GGCCCAGGCC 
AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC AGTGCCCTGG GCAAGGCCAC CAACTCGGAG 
GACGTGAAGG AGGAAGAGGA GGCCAAACAG TATCGAGAGT TCCAGAACCG ACAAGTACAG 
AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA GATGTGGAGA CCAAGCGGAA GCTGGAGCAT 
CTACGACAGG CTCATCAGCG GCTCAAGGAG GTTGTCCTGG ATACACACAC AACACAGTTC 
AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG AAGAAGGAAC TCCAGAAGAT CCTGGACAGG 
AAGCGCAACA ACAGCATCTC AGAGGCCAAG ACAAGGGAGA AACACAAGAA GGAGGTGGAA 
CTGACAGAGA TTAATCGGCG GCACATCACT GAGTCGGTAA ACTCCATCAG ACGGCTGGAA 
GAGGCCCAGA AGCAGCGCCA TGAACGCCTG GTGGCAGGGC AACAGCAAGT CCTCCAGCAG 
CTAGAGGAAG AGGAACCCAA GCTGCTGGCC CAGCTGACCC AGGAGTGTCA GGAACAGCGA 
GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC CTGCTGGGCG AGACAGCAGA GGGACTGGGG 
GACGGCCCCC TGGTGGCCTG TGCCAGCAAT GGTCATGCAC CTGGGAGTGG TGGGCACCTG 
TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277278.1
CDS	170..3874
Translation	MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL

Target ORF information:

RefSeq Version	NM_001290349.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290349.1

ORF Insert Sequence:

ATGGCGGGCG CGAGGCCCGG CGTCCACGCG CTGCAGCTGG AGCCGCCCAC CGTGGTGGAG 
ACCCTGCGGC GCGGGAGTAA GTTCATCAAA TGGGACGAGG AGGCCTCCAG TCGGAACCTG 
GTGACCCTGC GTGTGGACCC TAATGGCTTC TTCTTGTACT GGACAGGACC CAACATGGAG 
GTGGACACAC TGGACATCAG CTCCATCAGG GACACAAGGA CAGGTCGTTA TGCCCGCCTG 
CCCAAGGACC CTAAGATTCG AGAAGTACTG GGCTTTGGAG GTCCTGACAC CCGGCTGGAG 
GAGAAACTGA TGACAGTGGT GGCCGGGCCA GATCCAGTAA ATACCACATT CTTGAACTTC 
ATGGCCGTGC AAGATGACAC AGTCAAGGTC TGGTCAGAGG AGTTGTTTAA ACTGGCCATG 
AACATATTGG CTCAGAACGC CTCCCGGAAC ACCTTCCTGC GGAAAGCATA CACGAAGCTG 
AAGCTGCAGG TGAACCAGGA TGGACGGATT CCGGTCAAGA ACATTCTGAA GATGTTCTCG 
GCGGACAAGA AGCGGGTGGA GACGGCGCTG GAGTCCTGTG GCCTCAACTT CAACCGAAGT 
GAGTCCATTC GCCCTGATGA GTTTCCCTTG GAAATTTTTG AGCGGTTCTT GAATAAACTG 
TGTCTGCGCC CGGATATTGA CAAGATCCTG CTGGAGATAG GTGCCAAGGG CAAGCCGTAC 
CTCACTCTGG AGCAGCTCAT GGACTTTATC AACCAGAAGC AGAGAGACCC GAGACTCAAC 
GAAGTGCTGT ACCCGCCACT TCGGTCCTCA CAGGCTCGGC TGCTCATTGA GAAGTACGAG 
ACGAACAAAC AGTTCCTGGA GCGCGACCAG ATGTCTATGG AGGGCTTCAG CCGCTACCTG 
GGAGGGGAGG AGAATGGTAT CCTGCCTCTG GAGGCCCTGG ATCTGAGCAT GGACATGACC 
CAGCCACTGA GCGCATATTT TATCAACTCC TCACACAACA CCTATCTCAC TGCGGGCCAG 
CTGGCTGGAC CATCATCGGT GGAGATGTAC CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT 
GTGGAGCTGG ATGTATGGAA GGGACGGCCA CCGGAGGAAG AGCCTTTCAT CACTCATGGT 
TTCACCATGA CCACTGAGGT GCCATTGCGT GATGTGCTAG AGGCCATCGC TGAGGCAGCC 
TTCAAGACCT CGCCCTACCC TGTCATCCTC TCCTTTGAGA ACCATGTCGA CTCGGCAAAG 
CAGCAGGCCA AGATGGCTGA GTACTGCCGC TCTATCTTTG GGGATGCGCT GCTCATTGAC 
CCTCTCGACA AATACCCGCT ATCTGCGGGC ATCCCCCTGC CTAGTCCACA GGACCTGATG 
GGCCGTATCC TGGTGAAGAA TAAGAAGCGA CATCGGCCCA GCACAGGTGT CCCTGACAGC 
TCAGTGCGCA AGCGGCCTCT GGAACAGAGC AACTCGGCCT TGAGTGAGAG CTCGGCCGCC 
ACAGAGCCCT CCTCACCTCA GCTTGGGTCC CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC 
AATGGGGAGG AGGTGGGACT CGAGAAGACC AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG 
GAGAGCCTCA GCCGGGAACC TAATGTGCCC ATGCCTGACC GTGACCGAGA GGATGAGGAG 
GAAGATGAAG AAGAAGAGGA AACAACGGAT CCAAAAAAGC CCACCACTGA TGAGGGCACA 
GCCAGCAGTG AGGTCAATGC CACGGAGGAG ATGTCAACGC TCGTCAACTA CGTTGAGCCC 
GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA AAAAGGAACA AGTGCTTCGA GATGTCATCC 
TTCGTGGAGA CCAAGGCAAT GGAGCAACTG ACCAAGAGTC CTATGGAGTT TGTGGAATAC 
AACAAACAGC AGCTCAGCCG CATCTACCCT AAGGGCACAC GAGTGGACTC CTCCAACTAC 
ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC CAGCTTGTTG CCCTCAACTT CCAGACCTTG 
GATTTGCCAA TGCAGCTCAA CGCAGGCGTG TTTGAGTACA ATGGGCGCAG CGGGTACTTG 
CTCAAGCCCG AGTTTATGCG GCGGCCGGAC AAGTCTTTTG ATCCCTTCAC TGAGGTCATC 
GTAGATGGCA TAGTGGCCAA CGCCTTGCGG GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT 
GACAAGAAGG TGGGCATCTA CGTCGAGGTG GACATGTTTG GCCTCCCAGT TGACACAAGA 
CGCAAATATC GTACCCGGAC ATCCCAGGGG AACTCATTCA ACCCTGTGTG GGACGAGGAA 
CCCTTTGACT TCCCCAAGGT TGTGCTGCCC ACGCTGGCCT CACTTCGCAT CGCAGCCTTT 
GAGGAGGGTG GCAAATTTGT TGGGCACCGT ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA 
TACCACTATG TCTGCCTGCG AAACGAGGCC AACCAACCTT TGTGCCTGCC TGCCCTGCTT 
ATCTACACTG AAGCTTCTGA CTACATCCCA GATGACCACC AGGACTATGC GGAGGCCTTG 
ATTAACCCCA TCAAGCACGT AAGCCTAATG GACCAGCGGG CCAAGCAACT AGCCGCTCTC 
ATTGGGGAGA GTGAGGCTCA GGCCAGCACA GAGACATATC AGGAGACCCC GTGTCAACAG 
CCAGGGTCAC AGCTCCCCTC CAACCCCACA CCTAACCCAC TGGATGCCTC ACCTCGCTGG 
CCCCCCGGTC CTACCACTTC CTCCACTAGC TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT 
GATTTGATTG CCAGCATCCT CTCTGAGGTG ACCCCTACAC CTCTAGAGGA GCTCAGAAGC 
CACAAGGCTA TGGTGAAGCT ACGGAGCCGG CAGGATCGAG ACCTGCGGGA GTTGCATAAG 
AAGCACCAGC GCAAGGCTGT GGCCCTCACC CGCCGCCTTC TGGATGGCCT GGCCCAGGCC 
AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC AGTGCCCTGG GCAAGGCCAC CAACTCGGAG 
GACGTGAAGG AGGAAGAGGA GGCCAAACAG TATCGAGAGT TCCAGAACCG ACAAGTACAG 
AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA GATGTGGAGA CCAAGCGGAA GCTGGAGCAT 
CTACGACAGG CTCATCAGCG GCTCAAGGAG GTTGTCCTGG ATACACACAC AACACAGTTC 
AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG AAGAAGGAAC TCCAGAAGAT CCTGGACAGG 
AAGCGCAACA ACAGCATCTC AGAGGCCAAG ACAAGGGAGA AACACAAGAA GGAGGTGGAA 
CTGACAGAGA TTAATCGGCG GCACATCACT GAGTCGGTAA ACTCCATCAG ACGGCTGGAA 
GAGGCCCAGA AGCAGCGCCA TGAACGCCTG GTGGCAGGGC AACAGCAAGT CCTCCAGCAG 
CTAGAGGAAG AGGAACCCAA GCTGCTGGCC CAGCTGACCC AGGAGTGTCA GGAACAGCGA 
GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC CTGCTGGGCG AGACAGCAGA GGGACTGGGG 
GACGGCCCCC TGGTGGCCTG TGCCAGCAAT GGTCATGCAC CTGGGAGTGG TGGGCACCTG 
TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb3 ( XM_006526766.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526829.1 Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X1, mRNA.

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CloneID	OMu55309
Clone ID Related Accession (Same CDS sequence)	XM_006526766.3 , XM_006526766.4
Accession Version	XM_006526766.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGGA CCCCCACTTC AGTGTTGCCT GACCACCTCA CCCCCTATTC CAGCCTTGTT 
GCACTCCGGA AACTTGAGTC CCCTGTTGTC CTGGAGGCCT CCAGTCGGAA CCTGGTGACC 
CTGCGTGTGG ACCCTAATGG CTTCTTCTTG TACTGGACAG GACCCAACAT GGAGGTGGAC 
ACACTGGACA TCAGCTCCAT CAGGGACACA AGGACAGGTC GTTATGCCCG CCTGCCCAAG 
GACCCTAAGA TTCGAGAAGT ACTGGGCTTT GGAGGTCCTG ACACCCGGCT GGAGGAGAAA 
CTGATGACAG TGGTGGCCGG GCCAGATCCA GTAAATACCA CATTCTTGAA CTTCATGGCC 
GTGCAAGATG ACACAGTCAA GGTCTGGTCA GAGGAGTTGT TTAAACTGGC CATGAACATA 
TTGGCTCAGA ACGCCTCCCG GAACACCTTC CTGCGGAAAG CATACACGAA GCTGAAGCTG 
CAGGTGAACC AGGATGGACG GATTCCGGTC AAGAACATTC TGAAGATGTT CTCGGCGGAC 
AAGAAGCGGG TGGAGACGGC GCTGGAGTCC TGTGGCCTCA ACTTCAACCG AAGTGAGTCC 
ATTCGCCCTG ATGAGTTTCC CTTGGAAATT TTTGAGCGGT TCTTGAATAA ACTGTGTCTG 
CGCCCGGATA TTGACAAGAT CCTGCTGGAG ATAGGTGCCA AGGGCAAGCC GTACCTCACT 
CTGGAGCAGC TCATGGACTT TATCAACCAG AAGCAGAGAG ACCCGAGACT CAACGAAGTG 
CTGTACCCGC CACTTCGGTC CTCACAGGCT CGGCTGCTCA TTGAGAAGTA CGAGACGAAC 
AAACAGTTCC TGGAGCGCGA CCAGATGTCT ATGGAGGGCT TCAGCCGCTA CCTGGGAGGG 
GAGGAGAATG GTATCCTGCC TCTGGAGGCC CTGGATCTGA GCATGGACAT GACCCAGCCA 
CTGAGCGCAT ATTTTATCAA CTCCTCACAC AACACCTATC TCACTGCGGG CCAGCTGGCT 
GGACCATCAT CGGTGGAGAT GTACCGCCAG GCACTGCTGT GGGGCTGCCG CTGTGTGGAG 
CTGGATGTAT GGAAGGGACG GCCACCGGAG GAAGAGCCTT TCATCACTCA TGGTTTCACC 
ATGACCACTG AGGTGCCATT GCGTGATGTG CTAGAGGCCA TCGCTGAGGC AGCCTTCAAG 
ACCTCGCCCT ACCCTGTCAT CCTCTCCTTT GAGAACCATG TCGACTCGGC AAAGCAGCAG 
GCCAAGATGG CTGAGTACTG CCGCTCTATC TTTGGGGATG CGCTGCTCAT TGACCCTCTC 
GACAAATACC CGCTATCTGC GGGCATCCCC CTGCCTAGTC CACAGGACCT GATGGGCCGT 
ATCCTGGTGA AGAATAAGAA GCGACATCGG CCCAGCACAG GTGTCCCTGA CAGCTCAGTG 
CGCAAGCGGC CTCTGGAACA GAGCAACTCG GCCTTGAGTG AGAGCTCGGC CGCCACAGAG 
CCCTCCTCAC CTCAGCTTGG GTCCCCCAGC TCCGACAGCT GCCCTGGCCT AAGCAATGGG 
GAGGAGGTGG GACTCGAGAA GACCAGCCTG GAGCCTCAGA AGTCTCTAGG TGAGGAGAGC 
CTCAGCCGGG AACCTAATGT GCCCATGCCT GACCGTGACC GAGAGGATGA GGAGGAAGAT 
GAAGAAGAAG AGGAAACAAC GGATCCAAAA AAGCCCACCA CTGATGAGGG CACAGCCAGC 
AGTGAGGTCA ATGCCACGGA GGAGATGTCA ACGCTCGTCA ACTACGTTGA GCCCGTCAAG 
TTCAAGTCCT TCGAGGCTGC TCGAAAAAGG AACAAGTGCT TCGAGATGTC ATCCTTCGTG 
GAGACCAAGG CAATGGAGCA ACTGACCAAG AGTCCTATGG AGTTTGTGGA ATACAACAAA 
CAGCAGCTCA GCCGCATCTA CCCTAAGGGC ACACGAGTGG ACTCCTCCAA CTACATGCCT 
CAGCTCTTCT GGAACGTGGG TTGCCAGCTT GTTGCCCTCA ACTTCCAGAC CTTGGATTTG 
CCAATGCAGC TCAACGCAGG CGTGTTTGAG TACAATGGGC GCAGCGGGTA CTTGCTCAAG 
CCCGAGTTTA TGCGGCGGCC GGACAAGTCT TTTGATCCCT TCACTGAGGT CATCGTAGAT 
GGCATAGTGG CCAACGCCTT GCGGGTCAAG GTGATTTCGG GGCAGTTCCT GTCTGACAAG 
AAGGTGGGCA TCTACGTCGA GGTGGACATG TTTGGCCTCC CAGTTGACAC AAGACGCAAA 
TATCGTACCC GGACATCCCA GGGGAACTCA TTCAACCCTG TGTGGGACGA GGAACCCTTT 
GACTTCCCCA AGGTTGTGCT GCCCACGCTG GCCTCACTTC GCATCGCAGC CTTTGAGGAG 
GGTGGCAAAT TTGTTGGGCA CCGTATCCTG CCTGTCTCTG CTATCCGCTC AGGATACCAC 
TATGTCTGCC TGCGAAACGA GGCCAACCAA CCTTTGTGCC TGCCTGCCCT GCTTATCTAC 
ACTGAAGCTT CTGACTACAT CCCAGATGAC CACCAGGACT ATGCGGAGGC CTTGATTAAC 
CCCATCAAGC ACGTAAGCCT AATGGACCAG CGGGCCAAGC AACTAGCCGC TCTCATTGGG 
GAGAGTGAGG CTCAGGCCAG CACAGAGACA TATCAGGAGA CCCCGTGTCA ACAGCCAGGG 
TCACAGCTCC CCTCCAACCC CACACCTAAC CCACTGGATG CCTCACCTCG CTGGCCCCCC 
GGTCCTACCA CTTCCTCCAC TAGCTCCTCC CTCAGTAGCC CAGGGCAGCG AGATGATTTG 
ATTGCCAGCA TCCTCTCTGA GGTGACCCCT ACACCTCTAG AGGAGCTCAG AAGCCACAAG 
GCTATGGTGA AGCTACGGAG CCGGCAGGAT CGAGACCTGC GGGAGTTGCA TAAGAAGCAC 
CAGCGCAAGG CTGTGGCCCT CACCCGCCGC CTTCTGGATG GCCTGGCCCA GGCCAGGGCT 
GAGGGGAAGT GCCGGCCCTC CCCCAGTGCC CTGGGCAAGG CCACCAACTC GGAGGACGTG 
AAGGAGGAAG AGGAGGCCAA ACAGTATCGA GAGTTCCAGA ACCGACAAGT ACAGAGCCTG 
TTGGAGCTGA GGGAAGCCCA GGCAGATGTG GAGACCAAGC GGAAGCTGGA GCATCTACGA 
CAGGCTCATC AGCGGCTCAA GGAGGTTGTC CTGGATACAC ACACAACACA GTTCAAGAGG 
CTGAAGGAGC TGAATGAAAG GGAGAAGAAG GAACTCCAGA AGATCCTGGA CAGGAAGCGC 
AACAACAGCA TCTCAGAGGC CAAGACAAGG GAGAAACACA AGAAGGAGGT GGAACTGACA 
GAGATTAATC GGCGGCACAT CACTGAGTCG GTAAACTCCA TCAGACGGCT GGAAGAGGCC 
CAGAAGCAGC GCCATGAACG CCTGGTGGCA GGGCAACAGC AAGTCCTCCA GCAGCTAGAG 
GAAGAGGAAC CCAAGCTGCT GGCCCAGCTG ACCCAGGAGT GTCAGGAACA GCGAGAGAGG 
TTGCCCCAGG AGATCCGTCG GTGCCTGCTG GGCGAGACAG CAGAGGGACT GGGGGACGGC 
CCCCTGGTGG CCTGTGCCAG CAATGGTCAT GCACCTGGGA GTGGTGGGCA CCTGTCCAGC 
GCTGACTCGG AGAGCCAGGA GGAGAACACC CAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526829.1
CDS	164..3862
Translation	MPGTPTSVLPDHLTPYSSLVALRKLESPVVLEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL

Target ORF information:

RefSeq Version	XM_006526766.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526766.3

ORF Insert Sequence:

ATGCCAGGGA CCCCCACTTC AGTGTTGCCT GACCACCTCA CCCCCTATTC CAGCCTTGTT 
GCACTCCGGA AACTTGAGTC CCCTGTTGTC CTGGAGGCCT CCAGTCGGAA CCTGGTGACC 
CTGCGTGTGG ACCCTAATGG CTTCTTCTTG TACTGGACAG GACCCAACAT GGAGGTGGAC 
ACACTGGACA TCAGCTCCAT CAGGGACACA AGGACAGGTC GTTATGCCCG CCTGCCCAAG 
GACCCTAAGA TTCGAGAAGT ACTGGGCTTT GGAGGTCCTG ACACCCGGCT GGAGGAGAAA 
CTGATGACAG TGGTGGCCGG GCCAGATCCA GTAAATACCA CATTCTTGAA CTTCATGGCC 
GTGCAAGATG ACACAGTCAA GGTCTGGTCA GAGGAGTTGT TTAAACTGGC CATGAACATA 
TTGGCTCAGA ACGCCTCCCG GAACACCTTC CTGCGGAAAG CATACACGAA GCTGAAGCTG 
CAGGTGAACC AGGATGGACG GATTCCGGTC AAGAACATTC TGAAGATGTT CTCGGCGGAC 
AAGAAGCGGG TGGAGACGGC GCTGGAGTCC TGTGGCCTCA ACTTCAACCG AAGTGAGTCC 
ATTCGCCCTG ATGAGTTTCC CTTGGAAATT TTTGAGCGGT TCTTGAATAA ACTGTGTCTG 
CGCCCGGATA TTGACAAGAT CCTGCTGGAG ATAGGTGCCA AGGGCAAGCC GTACCTCACT 
CTGGAGCAGC TCATGGACTT TATCAACCAG AAGCAGAGAG ACCCGAGACT CAACGAAGTG 
CTGTACCCGC CACTTCGGTC CTCACAGGCT CGGCTGCTCA TTGAGAAGTA CGAGACGAAC 
AAACAGTTCC TGGAGCGCGA CCAGATGTCT ATGGAGGGCT TCAGCCGCTA CCTGGGAGGG 
GAGGAGAATG GTATCCTGCC TCTGGAGGCC CTGGATCTGA GCATGGACAT GACCCAGCCA 
CTGAGCGCAT ATTTTATCAA CTCCTCACAC AACACCTATC TCACTGCGGG CCAGCTGGCT 
GGACCATCAT CGGTGGAGAT GTACCGCCAG GCACTGCTGT GGGGCTGCCG CTGTGTGGAG 
CTGGATGTAT GGAAGGGACG GCCACCGGAG GAAGAGCCTT TCATCACTCA TGGTTTCACC 
ATGACCACTG AGGTGCCATT GCGTGATGTG CTAGAGGCCA TCGCTGAGGC AGCCTTCAAG 
ACCTCGCCCT ACCCTGTCAT CCTCTCCTTT GAGAACCATG TCGACTCGGC AAAGCAGCAG 
GCCAAGATGG CTGAGTACTG CCGCTCTATC TTTGGGGATG CGCTGCTCAT TGACCCTCTC 
GACAAATACC CGCTATCTGC GGGCATCCCC CTGCCTAGTC CACAGGACCT GATGGGCCGT 
ATCCTGGTGA AGAATAAGAA GCGACATCGG CCCAGCACAG GTGTCCCTGA CAGCTCAGTG 
CGCAAGCGGC CTCTGGAACA GAGCAACTCG GCCTTGAGTG AGAGCTCGGC CGCCACAGAG 
CCCTCCTCAC CTCAGCTTGG GTCCCCCAGC TCCGACAGCT GCCCTGGCCT AAGCAATGGG 
GAGGAGGTGG GACTCGAGAA GACCAGCCTG GAGCCTCAGA AGTCTCTAGG TGAGGAGAGC 
CTCAGCCGGG AACCTAATGT GCCCATGCCT GACCGTGACC GAGAGGATGA GGAGGAAGAT 
GAAGAAGAAG AGGAAACAAC GGATCCAAAA AAGCCCACCA CTGATGAGGG CACAGCCAGC 
AGTGAGGTCA ATGCCACGGA GGAGATGTCA ACGCTCGTCA ACTACGTTGA GCCCGTCAAG 
TTCAAGTCCT TCGAGGCTGC TCGAAAAAGG AACAAGTGCT TCGAGATGTC ATCCTTCGTG 
GAGACCAAGG CAATGGAGCA ACTGACCAAG AGTCCTATGG AGTTTGTGGA ATACAACAAA 
CAGCAGCTCA GCCGCATCTA CCCTAAGGGC ACACGAGTGG ACTCCTCCAA CTACATGCCT 
CAGCTCTTCT GGAACGTGGG TTGCCAGCTT GTTGCCCTCA ACTTCCAGAC CTTGGATTTG 
CCAATGCAGC TCAACGCAGG CGTGTTTGAG TACAATGGGC GCAGCGGGTA CTTGCTCAAG 
CCCGAGTTTA TGCGGCGGCC GGACAAGTCT TTTGATCCCT TCACTGAGGT CATCGTAGAT 
GGCATAGTGG CCAACGCCTT GCGGGTCAAG GTGATTTCGG GGCAGTTCCT GTCTGACAAG 
AAGGTGGGCA TCTACGTCGA GGTGGACATG TTTGGCCTCC CAGTTGACAC AAGACGCAAA 
TATCGTACCC GGACATCCCA GGGGAACTCA TTCAACCCTG TGTGGGACGA GGAACCCTTT 
GACTTCCCCA AGGTTGTGCT GCCCACGCTG GCCTCACTTC GCATCGCAGC CTTTGAGGAG 
GGTGGCAAAT TTGTTGGGCA CCGTATCCTG CCTGTCTCTG CTATCCGCTC AGGATACCAC 
TATGTCTGCC TGCGAAACGA GGCCAACCAA CCTTTGTGCC TGCCTGCCCT GCTTATCTAC 
ACTGAAGCTT CTGACTACAT CCCAGATGAC CACCAGGACT ATGCGGAGGC CTTGATTAAC 
CCCATCAAGC ACGTAAGCCT AATGGACCAG CGGGCCAAGC AACTAGCCGC TCTCATTGGG 
GAGAGTGAGG CTCAGGCCAG CACAGAGACA TATCAGGAGA CCCCGTGTCA ACAGCCAGGG 
TCACAGCTCC CCTCCAACCC CACACCTAAC CCACTGGATG CCTCACCTCG CTGGCCCCCC 
GGTCCTACCA CTTCCTCCAC TAGCTCCTCC CTCAGTAGCC CAGGGCAGCG AGATGATTTG 
ATTGCCAGCA TCCTCTCTGA GGTGACCCCT ACACCTCTAG AGGAGCTCAG AAGCCACAAG 
GCTATGGTGA AGCTACGGAG CCGGCAGGAT CGAGACCTGC GGGAGTTGCA TAAGAAGCAC 
CAGCGCAAGG CTGTGGCCCT CACCCGCCGC CTTCTGGATG GCCTGGCCCA GGCCAGGGCT 
GAGGGGAAGT GCCGGCCCTC CCCCAGTGCC CTGGGCAAGG CCACCAACTC GGAGGACGTG 
AAGGAGGAAG AGGAGGCCAA ACAGTATCGA GAGTTCCAGA ACCGACAAGT ACAGAGCCTG 
TTGGAGCTGA GGGAAGCCCA GGCAGATGTG GAGACCAAGC GGAAGCTGGA GCATCTACGA 
CAGGCTCATC AGCGGCTCAA GGAGGTTGTC CTGGATACAC ACACAACACA GTTCAAGAGG 
CTGAAGGAGC TGAATGAAAG GGAGAAGAAG GAACTCCAGA AGATCCTGGA CAGGAAGCGC 
AACAACAGCA TCTCAGAGGC CAAGACAAGG GAGAAACACA AGAAGGAGGT GGAACTGACA 
GAGATTAATC GGCGGCACAT CACTGAGTCG GTAAACTCCA TCAGACGGCT GGAAGAGGCC 
CAGAAGCAGC GCCATGAACG CCTGGTGGCA GGGCAACAGC AAGTCCTCCA GCAGCTAGAG 
GAAGAGGAAC CCAAGCTGCT GGCCCAGCTG ACCCAGGAGT GTCAGGAACA GCGAGAGAGG 
TTGCCCCAGG AGATCCGTCG GTGCCTGCTG GGCGAGACAG CAGAGGGACT GGGGGACGGC 
CCCCTGGTGG CCTGTGCCAG CAATGGTCAT GCACCTGGGA GTGGTGGGCA CCTGTCCAGC 
GCTGACTCGG AGAGCCAGGA GGAGAACACC CAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb3 ( XM_006526767.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526830.1 Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X2, mRNA.

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CloneID	OMu55310
Clone ID Related Accession (Same CDS sequence)	XM_006526767.4 , XM_006526767.3
Accession Version	XM_006526767.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAGG AGGCCTCCAG TCGGAACCTG GTGACCCTGC GTGTGGACCC TAATGGCTTC 
TTCTTGTACT GGACAGGACC CAACATGGAG GTGGACACAC TGGACATCAG CTCCATCAGG 
GACACAAGGA CAGGTCGTTA TGCCCGCCTG CCCAAGGACC CTAAGATTCG AGAAGTACTG 
GGCTTTGGAG GTCCTGACAC CCGGCTGGAG GAGAAACTGA TGACAGTGGT GGCCGGGCCA 
GATCCAGTAA ATACCACATT CTTGAACTTC ATGGCCGTGC AAGATGACAC AGTCAAGGTC 
TGGTCAGAGG AGTTGTTTAA ACTGGCCATG AACATATTGG CTCAGAACGC CTCCCGGAAC 
ACCTTCCTGC GGAAAGCATA CACGAAGCTG AAGCTGCAGG TGAACCAGGA TGGACGGATT 
CCGGTCAAGA ACATTCTGAA GATGTTCTCG GCGGACAAGA AGCGGGTGGA GACGGCGCTG 
GAGTCCTGTG GCCTCAACTT CAACCGAAGT GAGTCCATTC GCCCTGATGA GTTTCCCTTG 
GAAATTTTTG AGCGGTTCTT GAATAAACTG TGTCTGCGCC CGGATATTGA CAAGATCCTG 
CTGGAGATAG GTGCCAAGGG CAAGCCGTAC CTCACTCTGG AGCAGCTCAT GGACTTTATC 
AACCAGAAGC AGAGAGACCC GAGACTCAAC GAAGTGCTGT ACCCGCCACT TCGGTCCTCA 
CAGGCTCGGC TGCTCATTGA GAAGTACGAG ACGAACAAAC AGTTCCTGGA GCGCGACCAG 
ATGTCTATGG AGGGCTTCAG CCGCTACCTG GGAGGGGAGG AGAATGGTAT CCTGCCTCTG 
GAGGCCCTGG ATCTGAGCAT GGACATGACC CAGCCACTGA GCGCATATTT TATCAACTCC 
TCACACAACA CCTATCTCAC TGCGGGCCAG CTGGCTGGAC CATCATCGGT GGAGATGTAC 
CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT GTGGAGCTGG ATGTATGGAA GGGACGGCCA 
CCGGAGGAAG AGCCTTTCAT CACTCATGGT TTCACCATGA CCACTGAGGT GCCATTGCGT 
GATGTGCTAG AGGCCATCGC TGAGGCAGCC TTCAAGACCT CGCCCTACCC TGTCATCCTC 
TCCTTTGAGA ACCATGTCGA CTCGGCAAAG CAGCAGGCCA AGATGGCTGA GTACTGCCGC 
TCTATCTTTG GGGATGCGCT GCTCATTGAC CCTCTCGACA AATACCCGCT ATCTGCGGGC 
ATCCCCCTGC CTAGTCCACA GGACCTGATG GGCCGTATCC TGGTGAAGAA TAAGAAGCGA 
CATCGGCCCA GCACAGGTGT CCCTGACAGC TCAGTGCGCA AGCGGCCTCT GGAACAGAGC 
AACTCGGCCT TGAGTGAGAG CTCGGCCGCC ACAGAGCCCT CCTCACCTCA GCTTGGGTCC 
CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC AATGGGGAGG AGGTGGGACT CGAGAAGACC 
AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG GAGAGCCTCA GCCGGGAACC TAATGTGCCC 
ATGCCTGACC GTGACCGAGA GGATGAGGAG GAAGATGAAG AAGAAGAGGA AACAACGGAT 
CCAAAAAAGC CCACCACTGA TGAGGGCACA GCCAGCAGTG AGGTCAATGC CACGGAGGAG 
ATGTCAACGC TCGTCAACTA CGTTGAGCCC GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA 
AAAAGGAACA AGTGCTTCGA GATGTCATCC TTCGTGGAGA CCAAGGCAAT GGAGCAACTG 
ACCAAGAGTC CTATGGAGTT TGTGGAATAC AACAAACAGC AGCTCAGCCG CATCTACCCT 
AAGGGCACAC GAGTGGACTC CTCCAACTAC ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC 
CAGCTTGTTG CCCTCAACTT CCAGACCTTG GATTTGCCAA TGCAGCTCAA CGCAGGCGTG 
TTTGAGTACA ATGGGCGCAG CGGGTACTTG CTCAAGCCCG AGTTTATGCG GCGGCCGGAC 
AAGTCTTTTG ATCCCTTCAC TGAGGTCATC GTAGATGGCA TAGTGGCCAA CGCCTTGCGG 
GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT GACAAGAAGG TGGGCATCTA CGTCGAGGTG 
GACATGTTTG GCCTCCCAGT TGACACAAGA CGCAAATATC GTACCCGGAC ATCCCAGGGG 
AACTCATTCA ACCCTGTGTG GGACGAGGAA CCCTTTGACT TCCCCAAGGT TGTGCTGCCC 
ACGCTGGCCT CACTTCGCAT CGCAGCCTTT GAGGAGGGTG GCAAATTTGT TGGGCACCGT 
ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA TACCACTATG TCTGCCTGCG AAACGAGGCC 
AACCAACCTT TGTGCCTGCC TGCCCTGCTT ATCTACACTG AAGCTTCTGA CTACATCCCA 
GATGACCACC AGGACTATGC GGAGGCCTTG ATTAACCCCA TCAAGCACGT AAGCCTAATG 
GACCAGCGGG CCAAGCAACT AGCCGCTCTC ATTGGGGAGA GTGAGGCTCA GGCCAGCACA 
GAGACATATC AGGAGACCCC GTGTCAACAG CCAGGGTCAC AGCTCCCCTC CAACCCCACA 
CCTAACCCAC TGGATGCCTC ACCTCGCTGG CCCCCCGGTC CTACCACTTC CTCCACTAGC 
TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT GATTTGATTG CCAGCATCCT CTCTGAGGTG 
ACCCCTACAC CTCTAGAGGA GCTCAGAAGC CACAAGGCTA TGGTGAAGCT ACGGAGCCGG 
CAGGATCGAG ACCTGCGGGA GTTGCATAAG AAGCACCAGC GCAAGGCTGT GGCCCTCACC 
CGCCGCCTTC TGGATGGCCT GGCCCAGGCC AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC 
AGTGCCCTGG GCAAGGCCAC CAACTCGGAG GACGTGAAGG AGGAAGAGGA GGCCAAACAG 
TATCGAGAGT TCCAGAACCG ACAAGTACAG AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA 
GATGTGGAGA CCAAGCGGAA GCTGGAGCAT CTACGACAGG CTCATCAGCG GCTCAAGGAG 
GTTGTCCTGG ATACACACAC AACACAGTTC AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG 
AAGAAGGAAC TCCAGAAGAT CCTGGACAGG AAGCGCAACA ACAGCATCTC AGAGGCCAAG 
ACAAGGGAGA AACACAAGAA GGAGGTGGAA CTGACAGAGA TTAATCGGCG GCACATCACT 
GAGTCGGTAA ACTCCATCAG ACGGCTGGAA GAGGCCCAGA AGCAGCGCCA TGAACGCCTG 
GTGGCAGGGC AACAGCAAGT CCTCCAGCAG CTAGAGGAAG AGGAACCCAA GCTGCTGGCC 
CAGCTGACCC AGGAGTGTCA GGAACAGCGA GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC 
CTGCTGGGCG AGACAGCAGA GGGACTGGGG GACGGCCCCC TGGTGGCCTG TGCCAGCAAT 
GGTCATGCAC CTGGGAGTGG TGGGCACCTG TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG 
AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526830.1
CDS	118..3732
Translation	MIKEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL

Target ORF information:

RefSeq Version	XM_006526767.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 3 (Plcb3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526767.3

ORF Insert Sequence:

ATGATAAAGG AGGCCTCCAG TCGGAACCTG GTGACCCTGC GTGTGGACCC TAATGGCTTC 
TTCTTGTACT GGACAGGACC CAACATGGAG GTGGACACAC TGGACATCAG CTCCATCAGG 
GACACAAGGA CAGGTCGTTA TGCCCGCCTG CCCAAGGACC CTAAGATTCG AGAAGTACTG 
GGCTTTGGAG GTCCTGACAC CCGGCTGGAG GAGAAACTGA TGACAGTGGT GGCCGGGCCA 
GATCCAGTAA ATACCACATT CTTGAACTTC ATGGCCGTGC AAGATGACAC AGTCAAGGTC 
TGGTCAGAGG AGTTGTTTAA ACTGGCCATG AACATATTGG CTCAGAACGC CTCCCGGAAC 
ACCTTCCTGC GGAAAGCATA CACGAAGCTG AAGCTGCAGG TGAACCAGGA TGGACGGATT 
CCGGTCAAGA ACATTCTGAA GATGTTCTCG GCGGACAAGA AGCGGGTGGA GACGGCGCTG 
GAGTCCTGTG GCCTCAACTT CAACCGAAGT GAGTCCATTC GCCCTGATGA GTTTCCCTTG 
GAAATTTTTG AGCGGTTCTT GAATAAACTG TGTCTGCGCC CGGATATTGA CAAGATCCTG 
CTGGAGATAG GTGCCAAGGG CAAGCCGTAC CTCACTCTGG AGCAGCTCAT GGACTTTATC 
AACCAGAAGC AGAGAGACCC GAGACTCAAC GAAGTGCTGT ACCCGCCACT TCGGTCCTCA 
CAGGCTCGGC TGCTCATTGA GAAGTACGAG ACGAACAAAC AGTTCCTGGA GCGCGACCAG 
ATGTCTATGG AGGGCTTCAG CCGCTACCTG GGAGGGGAGG AGAATGGTAT CCTGCCTCTG 
GAGGCCCTGG ATCTGAGCAT GGACATGACC CAGCCACTGA GCGCATATTT TATCAACTCC 
TCACACAACA CCTATCTCAC TGCGGGCCAG CTGGCTGGAC CATCATCGGT GGAGATGTAC 
CGCCAGGCAC TGCTGTGGGG CTGCCGCTGT GTGGAGCTGG ATGTATGGAA GGGACGGCCA 
CCGGAGGAAG AGCCTTTCAT CACTCATGGT TTCACCATGA CCACTGAGGT GCCATTGCGT 
GATGTGCTAG AGGCCATCGC TGAGGCAGCC TTCAAGACCT CGCCCTACCC TGTCATCCTC 
TCCTTTGAGA ACCATGTCGA CTCGGCAAAG CAGCAGGCCA AGATGGCTGA GTACTGCCGC 
TCTATCTTTG GGGATGCGCT GCTCATTGAC CCTCTCGACA AATACCCGCT ATCTGCGGGC 
ATCCCCCTGC CTAGTCCACA GGACCTGATG GGCCGTATCC TGGTGAAGAA TAAGAAGCGA 
CATCGGCCCA GCACAGGTGT CCCTGACAGC TCAGTGCGCA AGCGGCCTCT GGAACAGAGC 
AACTCGGCCT TGAGTGAGAG CTCGGCCGCC ACAGAGCCCT CCTCACCTCA GCTTGGGTCC 
CCCAGCTCCG ACAGCTGCCC TGGCCTAAGC AATGGGGAGG AGGTGGGACT CGAGAAGACC 
AGCCTGGAGC CTCAGAAGTC TCTAGGTGAG GAGAGCCTCA GCCGGGAACC TAATGTGCCC 
ATGCCTGACC GTGACCGAGA GGATGAGGAG GAAGATGAAG AAGAAGAGGA AACAACGGAT 
CCAAAAAAGC CCACCACTGA TGAGGGCACA GCCAGCAGTG AGGTCAATGC CACGGAGGAG 
ATGTCAACGC TCGTCAACTA CGTTGAGCCC GTCAAGTTCA AGTCCTTCGA GGCTGCTCGA 
AAAAGGAACA AGTGCTTCGA GATGTCATCC TTCGTGGAGA CCAAGGCAAT GGAGCAACTG 
ACCAAGAGTC CTATGGAGTT TGTGGAATAC AACAAACAGC AGCTCAGCCG CATCTACCCT 
AAGGGCACAC GAGTGGACTC CTCCAACTAC ATGCCTCAGC TCTTCTGGAA CGTGGGTTGC 
CAGCTTGTTG CCCTCAACTT CCAGACCTTG GATTTGCCAA TGCAGCTCAA CGCAGGCGTG 
TTTGAGTACA ATGGGCGCAG CGGGTACTTG CTCAAGCCCG AGTTTATGCG GCGGCCGGAC 
AAGTCTTTTG ATCCCTTCAC TGAGGTCATC GTAGATGGCA TAGTGGCCAA CGCCTTGCGG 
GTCAAGGTGA TTTCGGGGCA GTTCCTGTCT GACAAGAAGG TGGGCATCTA CGTCGAGGTG 
GACATGTTTG GCCTCCCAGT TGACACAAGA CGCAAATATC GTACCCGGAC ATCCCAGGGG 
AACTCATTCA ACCCTGTGTG GGACGAGGAA CCCTTTGACT TCCCCAAGGT TGTGCTGCCC 
ACGCTGGCCT CACTTCGCAT CGCAGCCTTT GAGGAGGGTG GCAAATTTGT TGGGCACCGT 
ATCCTGCCTG TCTCTGCTAT CCGCTCAGGA TACCACTATG TCTGCCTGCG AAACGAGGCC 
AACCAACCTT TGTGCCTGCC TGCCCTGCTT ATCTACACTG AAGCTTCTGA CTACATCCCA 
GATGACCACC AGGACTATGC GGAGGCCTTG ATTAACCCCA TCAAGCACGT AAGCCTAATG 
GACCAGCGGG CCAAGCAACT AGCCGCTCTC ATTGGGGAGA GTGAGGCTCA GGCCAGCACA 
GAGACATATC AGGAGACCCC GTGTCAACAG CCAGGGTCAC AGCTCCCCTC CAACCCCACA 
CCTAACCCAC TGGATGCCTC ACCTCGCTGG CCCCCCGGTC CTACCACTTC CTCCACTAGC 
TCCTCCCTCA GTAGCCCAGG GCAGCGAGAT GATTTGATTG CCAGCATCCT CTCTGAGGTG 
ACCCCTACAC CTCTAGAGGA GCTCAGAAGC CACAAGGCTA TGGTGAAGCT ACGGAGCCGG 
CAGGATCGAG ACCTGCGGGA GTTGCATAAG AAGCACCAGC GCAAGGCTGT GGCCCTCACC 
CGCCGCCTTC TGGATGGCCT GGCCCAGGCC AGGGCTGAGG GGAAGTGCCG GCCCTCCCCC 
AGTGCCCTGG GCAAGGCCAC CAACTCGGAG GACGTGAAGG AGGAAGAGGA GGCCAAACAG 
TATCGAGAGT TCCAGAACCG ACAAGTACAG AGCCTGTTGG AGCTGAGGGA AGCCCAGGCA 
GATGTGGAGA CCAAGCGGAA GCTGGAGCAT CTACGACAGG CTCATCAGCG GCTCAAGGAG 
GTTGTCCTGG ATACACACAC AACACAGTTC AAGAGGCTGA AGGAGCTGAA TGAAAGGGAG 
AAGAAGGAAC TCCAGAAGAT CCTGGACAGG AAGCGCAACA ACAGCATCTC AGAGGCCAAG 
ACAAGGGAGA AACACAAGAA GGAGGTGGAA CTGACAGAGA TTAATCGGCG GCACATCACT 
GAGTCGGTAA ACTCCATCAG ACGGCTGGAA GAGGCCCAGA AGCAGCGCCA TGAACGCCTG 
GTGGCAGGGC AACAGCAAGT CCTCCAGCAG CTAGAGGAAG AGGAACCCAA GCTGCTGGCC 
CAGCTGACCC AGGAGTGTCA GGAACAGCGA GAGAGGTTGC CCCAGGAGAT CCGTCGGTGC 
CTGCTGGGCG AGACAGCAGA GGGACTGGGG GACGGCCCCC TGGTGGCCTG TGCCAGCAAT 
GGTCATGCAC CTGGGAGTGG TGGGCACCTG TCCAGCGCTG ACTCGGAGAG CCAGGAGGAG 
AACACCCAGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The heterotrimeric G protein G?
The Journal of biological chemistry292(32)13133-13142(2017 08)
Pradhan S,Khatlani T,Nairn AC,Vijayan KV

Synergistic Signaling by Light and Acetylcholine in Mouse Iris Sphincter Muscle.
Current biology : CB27(12)1791-1800.e5(2017 Jun)
Wang Q,Yue WWS,Jiang Z,Xue T,Kang SH,Bergles DE,Mikoshiba K,Offermanns S,Yau KW

Gnb isoforms control a signaling pathway comprising Rac1, Plc?2, and Plc?3 leading to LFA-1 activation and neutrophil arrest in vivo.
Blood127(3)314-24(2016 Jan)
Block H,Stadtmann A,Riad D,Rossaint J,Sohlbach C,Germena G,Wu D,Simon SI,Ley K,Zarbock A

Macrophages monitor tissue osmolarity and induce inflammatory response through NLRP3 and NLRC4 inflammasome activation.
Nature communications66931(2015 May)
Ip WK,Medzhitov R

Critical role for mast cell Stat5 activity in skin inflammation.
Cell reports6(2)366-76(2014 Jan)
Ando T,Xiao W,Gao P,Nam S,Matsumoto K,Tomimori Y,Hong H,Yamashita H,Kimura M,Kashiwakura J,Hata TR,Izuhara K,Gurish MF,Roers A,Rafaels NM,Barnes KC,Jamora C,Kawakami Y,Kawakami T

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Plcb3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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