Plcb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plcb1
Entrez Gene ID	18795
Full Name	phospholipase C, beta 1
Synonyms	3110043I21Rik,AI132408,Plcb,mKIAA0581
General protein information	Preferred Names phospholipase C, beta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 PLC-154 PLC-beta-1 phosphoinositide phospholipase C phosphoinositide phospholipase C-beta-1 phospholipase C-beta-1a
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F3\|2 65.66 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006498930.2	XP_006498993.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
XM_006498930.3	XP_006498993.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
NM_019677.2	NP_062651.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform 2
NM_001145830.1	NP_001139302.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform 1

Pathways from BioSystems

Homologies

	PLCB1	XP_001116017.1
Mus musculus (house mouse)	Plcb1	NP_001139302.1
Canis lupus familiaris (dog)	PLCB1	XP_005634858.1
Bos taurus (cattle)	PLCB1	NP_777242.1
Gallus gallus (chicken)	PLCB1	XP_425261.3
Drosophila melanogaster (fruit fly)	Plc21C	NP_995604.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009115	XP_319865.4
Homo sapiens (human)	PLCB1	NP_056007.1
Pan troglodytes (chimpanzee)	PLCB1	XP_001170216.3
Rattus norvegicus (Norway rat)	Plcb1	NP_001071109.1

Gene Ontology
Bibliography

Related articles in PubMed

Synergistic Signaling by Light and Acetylcholine in Mouse Iris Sphincter Muscle.
Wang Q, Yue WWS, Jiang Z, Xue T, Kang SH, Bergles DE, Mikoshiba K, Offermanns S, Yau KW
Current biology : CB27(12)1791-1800.e5(2017 Jun)

PLC-?1 and cell differentiation: An insight into myogenesis and osteogenesis.
Ramazzotti G, Faenza I, Fiume R, Billi AM, Manzoli L, Mongiorgi S, Ratti S, McCubrey JA, Suh PG, Cocco L, Follo MY
Advances in biological regulation631-5(2017 Jan)

IPMK and ?-catenin mediate PLC-?1-dependent signaling in myogenic differentiation.
Ramazzotti G, Billi AM, Manzoli L, Mazzetti C, Ruggeri A, Erneux C, Kim S, Suh PG, Cocco L, Faenza I
Oncotarget7(51)84118-84127(2016 Dec)

Phospholipase C?1 induces membrane tubulation and is involved in caveolae formation.
Inaba T, Kishimoto T, Murate M, Tajima T, Sakai S, Abe M, Makino A, Tomishige N, Ishitsuka R, Ikeda Y, Takeoka S, Kobayashi T
Proceedings of the National Academy of Sciences of the United States of America113(28)7834-9(2016 07)

MLP and CARP are linked to chronic PKC? signalling in dilated cardiomyopathy.
Lange S, Gehmlich K, Lun AS, Blondelle J, Hooper C, Dalton ND, Alvarez EA, Zhang X, Bang ML, Abassi YA, Dos Remedios CG, Peterson KL, Chen J, Ehler E
Nature communications712120(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Plcb1 plays a crucial role in the initiation of the genetic program responsible for muscle differentiation and osteogenesis. (Review)
Title: PLC-?1 and cell differentiation: An insight into myogenesis and osteogenesis.

functions in plasma membrane remodeling, particularly caveolae formation
Title: Phospholipase C?1 induces membrane tubulation and is involved in caveolae formation.

Unpredictable chronic mild stress mice exhibited lower nucleus accumbens (NAC) shell levels of phospholipase Cbeta.
Title: Homer2 regulates alcohol and stress cross-sensitization.

inhibition of miR-214 in C2C12 cells significantly enhances the protein level of PLCbeta1 and promotes C2C12 BMP-2-induced osteogenesis by targeting PLCbeta1
Title: BMP-2 Induced Expression of PLC?1 That is a Positive Regulator of Osteoblast Differentiation.

Heightened PLCbeta1b activity observed in diseased myocardium contributes to pathology by PKCalpha-mediated contractile dysfunction.
Title: The atypical 'b' splice variant of phospholipase C?1 promotes cardiac contractile dysfunction.

The following Plcb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plcb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16155	NM_001145830.1 Latest version!	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$713.30 $1019.00
OMu30113	XM_006498930.3 Latest version!	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu15960	NM_019677.2 Latest version!	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu30113	XM_006498930.2	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plcb1 ( NM_001145830.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139302.1 Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16155
Clone ID Related Accession (Same CDS sequence)	NM_001145830.1
Accession Version	NM_001145830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143153.1, BC058710.1 and AL935278.7. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.219817.1, SRR1660815.44070.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT TGTTACTCCA 
ATTATTTTGA GGACTGATCC TCAGGGATTT TTCTTTTACT GGACAGACCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTGCG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGACCAGAC CTGGTGAACA TTTCCCACTT GAATCTTGTG 
GCTTTTCAAG AGGAAGTAGC CAAGGAATGG ACAAATGAGG TTTTCAGCTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTCCAGGTCA CCCCAGAAGG CCGCATTCCT CTTAAAAACA TCTATCGATT GTTCTCAGCA 
GACCGGAAGC GCGTTGAAAC TGCACTAGAG GCTTGTAGTC TTCCATCTTC GAGGAATGAC 
TCCATTCCCC AAGAGGACTT CACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTCTGT 
CCCCGACCTG AAATTGATAA CATCTTTTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTG 
ACGGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTCAATGAA 
ATCCTTTACC CACCTCTGAA GCAGGAGCAG GTCCAAGTGT TGATTGAGAA ATATGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCG CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG AAGGACCGCT GAGGAAGAGC CTGTCATTAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACGTCTC CTTTCCCCAT CCTCCTTTCC TTTGAGAACC ACGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT GATGGAACCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCG CACAAGTCGT CAGAGGGAAG TGGTAAGAAG 
AAGCTTTCAG AGCAAGCTTC CAACACCTAC AGCGACTCCT CCAGTGTGTT CGAGCCTTCG 
TCTCCAGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA CGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACAGCT GGCAGCGAAG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAT TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTTGA ACAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATACAAC AAGATGCAAC TTAGCAGGAT ATATCCCAAG 
GGAACACGTG TGGATTCATC CAACTACATG CCTCAACTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAT 
GAGTACAATG GGAAGAGCGG CTATAGGCTG AAGCCAGAGT TCATGAGGAG GCCGGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TCTATCTGTT 
AAGATTATTT CAGGTCAGTT CCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGCT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ACCCTGTCTG GGAAGAAGAA CCTATTGTAT TCAAAAAGGT AGTTCTACCT 
TCTCTGGCCT GTTTGAGGAT AGCAGCATAT GAAGAGGGAG GCAAATTTAT TGGCCACCGG 
ATATTGCCTG TGCAGGCTAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGACGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA TTATGTGCCA 
GACACGTATG CAGATGTAAT CGAAGCTCTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGCT GGCTGCGTTG ACCCTGGAGG ATGAAGAGGA AGTAAAGAAG 
GAGGCTGACC CTGGAGAAAC ATCTTCCGAG GCTCCAAGCG AAACCAGGAC AACGCCAGCA 
GAGAATGGGG TGAATCACAC CGCATCCCTT GCACCCAAGC CGCCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCTGCCAAAA CAGAAGATCT GATCCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAAACCATC GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAACACTA CAAAGAAATG AAAGACCTGG TGAAGAGACA CCACAAGAAA 
ACCACGGAGC TCATTAAGGA GCACACGACC AAGTACAACG AGATTCAGAA TGACTACCTG 
AGAAGGAGGG CAGCCTTGGA GAAGTCCGCC AAAAAGGACA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCTG ACCACGGCTC ATCAGCCATT GAGCAAGACC TCGCTGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGCGA GAAGTACCAA AAGCGAGAAC ACATTAAACT GCTTATTCAG 
AAGTTGACAG ATGTTGCAGA AGAGTGTCAG AACAATCAGT TAAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA AGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCGTACATCC AGGAGGTGGT TCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TCGTGGAGAA ACACAATGAG ATCCGCCAGC AGATCCTCGA TGAGAAGCCC 
AAGCTGCAGA CGGAGCTGGA GCAAGAATAC CAAGACAAGT TCAAAAGGCT GCCCCTGGAG 
ATTCTGGAGT TTGTACAGGA AGCCATGAAA GGGAAGATCA GCGAGGACAG CAATCACGGC 
TCTGCCCCTC CCTCTCTGGC CTCAGATGCT GCAAAGGTGA ACCTCAAGTC TCCCTCCAGT 
GAGGAGATAG AAAGAGAGAA CCCAGGAAGA GAGTTTGATA CTCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139302.1
CDS	370..4020
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHNEIRQQILDEKPKLQTELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPPSLASDAAKVNLKSPSSEEIERENPGREFDTPL

Target ORF information:

RefSeq Version	NM_001145830.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145830.1

ORF Insert Sequence:

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT TGTTACTCCA 
ATTATTTTGA GGACTGATCC TCAGGGATTT TTCTTTTACT GGACAGACCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTGCG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGACCAGAC CTGGTGAACA TTTCCCACTT GAATCTTGTG 
GCTTTTCAAG AGGAAGTAGC CAAGGAATGG ACAAATGAGG TTTTCAGCTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTCCAGGTCA CCCCAGAAGG CCGCATTCCT CTTAAAAACA TCTATCGATT GTTCTCAGCA 
GACCGGAAGC GCGTTGAAAC TGCACTAGAG GCTTGTAGTC TTCCATCTTC GAGGAATGAC 
TCCATTCCCC AAGAGGACTT CACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTCTGT 
CCCCGACCTG AAATTGATAA CATCTTTTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTG 
ACGGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTCAATGAA 
ATCCTTTACC CACCTCTGAA GCAGGAGCAG GTCCAAGTGT TGATTGAGAA ATATGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCG CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG AAGGACCGCT GAGGAAGAGC CTGTCATTAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACGTCTC CTTTCCCCAT CCTCCTTTCC TTTGAGAACC ACGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT GATGGAACCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCG CACAAGTCGT CAGAGGGAAG TGGTAAGAAG 
AAGCTTTCAG AGCAAGCTTC CAACACCTAC AGCGACTCCT CCAGTGTGTT CGAGCCTTCG 
TCTCCAGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA CGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACAGCT GGCAGCGAAG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAT TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTTGA ACAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATACAAC AAGATGCAAC TTAGCAGGAT ATATCCCAAG 
GGAACACGTG TGGATTCATC CAACTACATG CCTCAACTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAT 
GAGTACAATG GGAAGAGCGG CTATAGGCTG AAGCCAGAGT TCATGAGGAG GCCGGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TCTATCTGTT 
AAGATTATTT CAGGTCAGTT CCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGCT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ACCCTGTCTG GGAAGAAGAA CCTATTGTAT TCAAAAAGGT AGTTCTACCT 
TCTCTGGCCT GTTTGAGGAT AGCAGCATAT GAAGAGGGAG GCAAATTTAT TGGCCACCGG 
ATATTGCCTG TGCAGGCTAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGACGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA TTATGTGCCA 
GACACGTATG CAGATGTAAT CGAAGCTCTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGCT GGCTGCGTTG ACCCTGGAGG ATGAAGAGGA AGTAAAGAAG 
GAGGCTGACC CTGGAGAAAC ATCTTCCGAG GCTCCAAGCG AAACCAGGAC AACGCCAGCA 
GAGAATGGGG TGAATCACAC CGCATCCCTT GCACCCAAGC CGCCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCTGCCAAAA CAGAAGATCT GATCCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAAACCATC GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAACACTA CAAAGAAATG AAAGACCTGG TGAAGAGACA CCACAAGAAA 
ACCACGGAGC TCATTAAGGA GCACACGACC AAGTACAACG AGATTCAGAA TGACTACCTG 
AGAAGGAGGG CAGCCTTGGA GAAGTCCGCC AAAAAGGACA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCTG ACCACGGCTC ATCAGCCATT GAGCAAGACC TCGCTGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGCGA GAAGTACCAA AAGCGAGAAC ACATTAAACT GCTTATTCAG 
AAGTTGACAG ATGTTGCAGA AGAGTGTCAG AACAATCAGT TAAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA AGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCGTACATCC AGGAGGTGGT TCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TCGTGGAGAA ACACAATGAG ATCCGCCAGC AGATCCTCGA TGAGAAGCCC 
AAGCTGCAGA CGGAGCTGGA GCAAGAATAC CAAGACAAGT TCAAAAGGCT GCCCCTGGAG 
ATTCTGGAGT TTGTACAGGA AGCCATGAAA GGGAAGATCA GCGAGGACAG CAATCACGGC 
TCTGCCCCTC CCTCTCTGGC CTCAGATGCT GCAAAGGTGA ACCTCAAGTC TCCCTCCAGT 
GAGGAGATAG AAAGAGAGAA CCCAGGAAGA GAGTTTGATA CTCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb1 ( XM_006498930.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498993.1 Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30113
Clone ID Related Accession (Same CDS sequence)	XM_006498930.2 , XM_006498930.3
Accession Version	XM_006498930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACACA TTAATGAGGA GACGGAGCTG TTAGATCTCA GCCTTGTCAA GGATGCCAGG 
TGTGGGAAGC ACGCCAAAGC TCCCAAGGAC CCCAAGTTGC GTGAACTTCT GGATGTTGGG 
AACATCGGAC ACTTGGAACA GCGCATGATA ACTGTGGTGT ATGGACCAGA CCTGGTGAAC 
ATTTCCCACT TGAATCTTGT GGCTTTTCAA GAGGAAGTAG CCAAGGAATG GACAAATGAG 
GTTTTCAGCT TGGCAACAAA CCTGCTGGCT CAGAACATGT CCAGGGACGC ATTTCTGGAG 
AAAGCATATA CTAAGCTCAA GCTCCAGGTC ACCCCAGAAG GCCGCATTCC TCTTAAAAAC 
ATCTATCGAT TGTTCTCAGC AGACCGGAAG CGCGTTGAAA CTGCACTAGA GGCTTGTAGT 
CTTCCATCTT CGAGGAATGA CTCCATTCCC CAAGAGGACT TCACTCCAGA TGTATACAGA 
GTTTTCCTGA ACAATCTCTG TCCCCGACCT GAAATTGATA ACATCTTTTC TGAATTTGGT 
GCCAAAAGCA AACCGTACCT GACGGTTGAT CAGATGATGG ATTTTATCAA CCTTAAGCAG 
AGAGATCCCC GGCTCAATGA AATCCTTTAC CCACCTCTGA AGCAGGAGCA GGTCCAAGTG 
TTGATTGAGA AATATGAGCC CAACAGCAGC CTCGCCAAGA AAGGGCAGAT GTCAGTGGAT 
GGATTCATGC GCTACCTGAG CGGAGAAGAA AATGGAGTCG TTTCACCTGA GAAACTGGAT 
TTGAACGAAG ACATGTCTCA GCCCCTGTCT CACTATTTCA TCAATTCCTC GCACAACACC 
TACCTCACAG CTGGCCAGTT GGCTGGGAAC TCGTCTGTAG AGATGTATCG CCAGGTGCTT 
CTGTCTGGAT GTCGCTGTGT GGAGCTGGAC TGCTGGAAGG GAAGGACCGC TGAGGAAGAG 
CCTGTCATTA CCCATGGATT CACCATGACA ACAGAAATAT CCTTCAAGGA AGTCATAGAA 
GCCATCGCAG AGTGTGCGTT CAAGACGTCT CCTTTCCCCA TCCTCCTTTC CTTTGAGAAC 
CACGTGGATT CCCCGAAGCA ACAAGCCAAG ATGGCCGAGT ATTGCCGATT AATCTTTGGT 
GATGCCCTCC TGATGGAACC ACTGGAAAAA TACCCACTGG AATCTGGGGT ACCTCTTCCA 
AGCCCTATGG ATTTAATGTA TAAAATCTTG GTGAAAAACA AGAAGAAGTC GCACAAGTCG 
TCAGAGGGAA GTGGTAAGAA GAAGCTTTCA GAGCAAGCTT CCAACACCTA CAGCGACTCC 
TCCAGTGTGT TCGAGCCTTC GTCTCCAGGA GCTGGGGAAG CAGATACGGA GAGTGATGAC 
GATGACGACG ACGATGACTG TAAAAAGTCT TCCATGGATG AGGGGACAGC TGGCAGCGAA 
GCCATGGCCA CAGAAGAGAT GTCTAACCTG GTGAACTATA TTCAGCCTGT CAAGTTTGAG 
TCCTTTGAAA TTTCAAAAAA AAGAAATAAA AGCTTTGAAA TGTCTTCCTT CGTGGAAACC 
AAAGGACTTG AACAACTTAC AAAGTCTCCG GTGGAATTTG TAGAATACAA CAAGATGCAA 
CTTAGCAGGA TATATCCCAA GGGAACACGT GTGGATTCAT CCAACTACAT GCCTCAACTC 
TTCTGGAATG CTGGCTGTCA GATGGTGGCG CTCAACTTCC AGACAGTGGA TCTAGCTATG 
CAGATAAACA TGGGCATGTA TGAGTACAAT GGGAAGAGCG GCTATAGGCT GAAGCCAGAG 
TTCATGAGGA GGCCGGACAA GCATTTTGAT CCATTTACTG AAGGAATCGT AGATGGGATA 
GTGGCCAACA CTCTATCTGT TAAGATTATT TCAGGTCAGT TCCTCTCTGA TAAGAAAGTT 
GGGACTTATG TGGAAGTGGA TATGTTTGGC TTGCCTGTGG ACACAAGGAG GAAGGCATTT 
AAAACCAAGA CATCCCAAGG AAATGCTGTA AACCCTGTCT GGGAAGAAGA ACCTATTGTA 
TTCAAAAAGG TAGTTCTACC TTCTCTGGCC TGTTTGAGGA TAGCAGCATA TGAAGAGGGA 
GGCAAATTTA TTGGCCACCG GATATTGCCT GTGCAGGCTA TTCGGCCAGG CTATCACTAC 
ATCTGCCTGC GGAATGAGAG GAACCAGCCC CTGACGCTGC CAGCTGTCTT TGTCTACATA 
GAAGTCAAAG ATTATGTGCC AGACACGTAT GCAGATGTAA TCGAAGCTCT ATCAAACCCA 
ATCCGATATG TCAATCTGAT GGAACAGAGA GCTAAGCAGC TGGCTGCGTT GACCCTGGAG 
GATGAAGAGG AAGTAAAGAA GGAGGCTGAC CCTGGAGAAA CATCTTCCGA GGCTCCAAGC 
GAAACCAGGA CAACGCCAGC AGAGAATGGG GTGAATCACA CCGCATCCCT TGCACCCAAG 
CCGCCTTCCC AGGCTCCACA CAGCCAGCCT GCTCCAGGGT CTGTGAAGGC ACCTGCCAAA 
ACAGAAGATC TGATCCAGAG CGTGTTAACA GAAGTAGAGG CGCAAACCAT CGAAGAGCTC 
AAGCAACAGA AATCGTTCGT GAAACTTCAA AAGAAACACT ACAAAGAAAT GAAAGACCTG 
GTGAAGAGAC ACCACAAGAA AACCACGGAG CTCATTAAGG AGCACACGAC CAAGTACAAC 
GAGATTCAGA ATGACTACCT GAGAAGGAGG GCAGCCTTGG AGAAGTCCGC CAAAAAGGAC 
AGCAAGAAGA AATCTGAACC CAGCAGCCCT GACCACGGCT CATCAGCCAT TGAGCAAGAC 
CTCGCTGCCC TGGATGCAGA AATGACTCAG AAGTTGATAG ACTTGAAAGA CAAGCAACAA 
CAGCAGCTGC TTAATCTTCG GCAAGAGCAG TATTACAGCG AGAAGTACCA AAAGCGAGAA 
CACATTAAAC TGCTTATTCA GAAGTTGACA GATGTTGCAG AAGAGTGTCA GAACAATCAG 
TTAAAGAAGC TGAAGGAAAT CTGCGAGAAA GAGAAGAAGG AATTAAAGAA GAAAATGGAT 
AAGAAGAGGC AAGAGAAGAT AACAGAAGCC AAGTCCAAAG ACAAAAGCCA GATGGAAGAG 
GAGAAGACAG AGATGATCCG ATCGTACATC CAGGAGGTGG TTCAGTACAT CAAGAGGTTA 
GAGGAAGCAC AAAGTAAAAG ACAAGAAAAA CTCGTGGAGA AACACAATGA GATCCGCCAG 
CAGATCCTCG ATGAGAAGCC CAAGCTGCAG ACGGAGCTGG AGCAAGAATA CCAAGACAAG 
TTCAAAAGGC TGCCCCTGGA GATTCTGGAG TTTGTACAGG AAGCCATGAA AGGGAAGATC 
AGCGAGGACA GCAATCACGG CTCTGCCCCT CCCTCTCTGG CCTCAGATGC TGCAAAGGTG 
AACCTCAAGT CTCCCTCCAG TGAGGAGATA GAAAGAGAGA ACCCAGGAAG AGAGTTTGAT 
ACTCCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498993.1
CDS	7800..11291
Translation	MKHINEETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHNEIRQQILDEKPKLQTELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPPSLASDAAKVNLKSPSSEEIERENPGREFDTPL

Target ORF information:

RefSeq Version	XM_006498930.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498930.3

ORF Insert Sequence:

ATGAAACACA TTAATGAGGA GACGGAGCTG TTAGATCTCA GCCTTGTCAA GGATGCCAGG 
TGTGGGAAGC ACGCCAAAGC TCCCAAGGAC CCCAAGTTGC GTGAACTTCT GGATGTTGGG 
AACATCGGAC ACTTGGAACA GCGCATGATA ACTGTGGTGT ATGGACCAGA CCTGGTGAAC 
ATTTCCCACT TGAATCTTGT GGCTTTTCAA GAGGAAGTAG CCAAGGAATG GACAAATGAG 
GTTTTCAGCT TGGCAACAAA CCTGCTGGCT CAGAACATGT CCAGGGACGC ATTTCTGGAG 
AAAGCATATA CTAAGCTCAA GCTCCAGGTC ACCCCAGAAG GCCGCATTCC TCTTAAAAAC 
ATCTATCGAT TGTTCTCAGC AGACCGGAAG CGCGTTGAAA CTGCACTAGA GGCTTGTAGT 
CTTCCATCTT CGAGGAATGA CTCCATTCCC CAAGAGGACT TCACTCCAGA TGTATACAGA 
GTTTTCCTGA ACAATCTCTG TCCCCGACCT GAAATTGATA ACATCTTTTC TGAATTTGGT 
GCCAAAAGCA AACCGTACCT GACGGTTGAT CAGATGATGG ATTTTATCAA CCTTAAGCAG 
AGAGATCCCC GGCTCAATGA AATCCTTTAC CCACCTCTGA AGCAGGAGCA GGTCCAAGTG 
TTGATTGAGA AATATGAGCC CAACAGCAGC CTCGCCAAGA AAGGGCAGAT GTCAGTGGAT 
GGATTCATGC GCTACCTGAG CGGAGAAGAA AATGGAGTCG TTTCACCTGA GAAACTGGAT 
TTGAACGAAG ACATGTCTCA GCCCCTGTCT CACTATTTCA TCAATTCCTC GCACAACACC 
TACCTCACAG CTGGCCAGTT GGCTGGGAAC TCGTCTGTAG AGATGTATCG CCAGGTGCTT 
CTGTCTGGAT GTCGCTGTGT GGAGCTGGAC TGCTGGAAGG GAAGGACCGC TGAGGAAGAG 
CCTGTCATTA CCCATGGATT CACCATGACA ACAGAAATAT CCTTCAAGGA AGTCATAGAA 
GCCATCGCAG AGTGTGCGTT CAAGACGTCT CCTTTCCCCA TCCTCCTTTC CTTTGAGAAC 
CACGTGGATT CCCCGAAGCA ACAAGCCAAG ATGGCCGAGT ATTGCCGATT AATCTTTGGT 
GATGCCCTCC TGATGGAACC ACTGGAAAAA TACCCACTGG AATCTGGGGT ACCTCTTCCA 
AGCCCTATGG ATTTAATGTA TAAAATCTTG GTGAAAAACA AGAAGAAGTC GCACAAGTCG 
TCAGAGGGAA GTGGTAAGAA GAAGCTTTCA GAGCAAGCTT CCAACACCTA CAGCGACTCC 
TCCAGTGTGT TCGAGCCTTC GTCTCCAGGA GCTGGGGAAG CAGATACGGA GAGTGATGAC 
GATGACGACG ACGATGACTG TAAAAAGTCT TCCATGGATG AGGGGACAGC TGGCAGCGAA 
GCCATGGCCA CAGAAGAGAT GTCTAACCTG GTGAACTATA TTCAGCCTGT CAAGTTTGAG 
TCCTTTGAAA TTTCAAAAAA AAGAAATAAA AGCTTTGAAA TGTCTTCCTT CGTGGAAACC 
AAAGGACTTG AACAACTTAC AAAGTCTCCG GTGGAATTTG TAGAATACAA CAAGATGCAA 
CTTAGCAGGA TATATCCCAA GGGAACACGT GTGGATTCAT CCAACTACAT GCCTCAACTC 
TTCTGGAATG CTGGCTGTCA GATGGTGGCG CTCAACTTCC AGACAGTGGA TCTAGCTATG 
CAGATAAACA TGGGCATGTA TGAGTACAAT GGGAAGAGCG GCTATAGGCT GAAGCCAGAG 
TTCATGAGGA GGCCGGACAA GCATTTTGAT CCATTTACTG AAGGAATCGT AGATGGGATA 
GTGGCCAACA CTCTATCTGT TAAGATTATT TCAGGTCAGT TCCTCTCTGA TAAGAAAGTT 
GGGACTTATG TGGAAGTGGA TATGTTTGGC TTGCCTGTGG ACACAAGGAG GAAGGCATTT 
AAAACCAAGA CATCCCAAGG AAATGCTGTA AACCCTGTCT GGGAAGAAGA ACCTATTGTA 
TTCAAAAAGG TAGTTCTACC TTCTCTGGCC TGTTTGAGGA TAGCAGCATA TGAAGAGGGA 
GGCAAATTTA TTGGCCACCG GATATTGCCT GTGCAGGCTA TTCGGCCAGG CTATCACTAC 
ATCTGCCTGC GGAATGAGAG GAACCAGCCC CTGACGCTGC CAGCTGTCTT TGTCTACATA 
GAAGTCAAAG ATTATGTGCC AGACACGTAT GCAGATGTAA TCGAAGCTCT ATCAAACCCA 
ATCCGATATG TCAATCTGAT GGAACAGAGA GCTAAGCAGC TGGCTGCGTT GACCCTGGAG 
GATGAAGAGG AAGTAAAGAA GGAGGCTGAC CCTGGAGAAA CATCTTCCGA GGCTCCAAGC 
GAAACCAGGA CAACGCCAGC AGAGAATGGG GTGAATCACA CCGCATCCCT TGCACCCAAG 
CCGCCTTCCC AGGCTCCACA CAGCCAGCCT GCTCCAGGGT CTGTGAAGGC ACCTGCCAAA 
ACAGAAGATC TGATCCAGAG CGTGTTAACA GAAGTAGAGG CGCAAACCAT CGAAGAGCTC 
AAGCAACAGA AATCGTTCGT GAAACTTCAA AAGAAACACT ACAAAGAAAT GAAAGACCTG 
GTGAAGAGAC ACCACAAGAA AACCACGGAG CTCATTAAGG AGCACACGAC CAAGTACAAC 
GAGATTCAGA ATGACTACCT GAGAAGGAGG GCAGCCTTGG AGAAGTCCGC CAAAAAGGAC 
AGCAAGAAGA AATCTGAACC CAGCAGCCCT GACCACGGCT CATCAGCCAT TGAGCAAGAC 
CTCGCTGCCC TGGATGCAGA AATGACTCAG AAGTTGATAG ACTTGAAAGA CAAGCAACAA 
CAGCAGCTGC TTAATCTTCG GCAAGAGCAG TATTACAGCG AGAAGTACCA AAAGCGAGAA 
CACATTAAAC TGCTTATTCA GAAGTTGACA GATGTTGCAG AAGAGTGTCA GAACAATCAG 
TTAAAGAAGC TGAAGGAAAT CTGCGAGAAA GAGAAGAAGG AATTAAAGAA GAAAATGGAT 
AAGAAGAGGC AAGAGAAGAT AACAGAAGCC AAGTCCAAAG ACAAAAGCCA GATGGAAGAG 
GAGAAGACAG AGATGATCCG ATCGTACATC CAGGAGGTGG TTCAGTACAT CAAGAGGTTA 
GAGGAAGCAC AAAGTAAAAG ACAAGAAAAA CTCGTGGAGA AACACAATGA GATCCGCCAG 
CAGATCCTCG ATGAGAAGCC CAAGCTGCAG ACGGAGCTGG AGCAAGAATA CCAAGACAAG 
TTCAAAAGGC TGCCCCTGGA GATTCTGGAG TTTGTACAGG AAGCCATGAA AGGGAAGATC 
AGCGAGGACA GCAATCACGG CTCTGCCCCT CCCTCTCTGG CCTCAGATGC TGCAAAGGTG 
AACCTCAAGT CTCCCTCCAG TGAGGAGATA GAAAGAGAGA ACCCAGGAAG AGAGTTTGAT 
ACTCCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb1 ( NM_019677.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062651.2 Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant 2, mRNA.

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CloneID	OMu15960
Clone ID Related Accession (Same CDS sequence)	NM_019677.2
Accession Version	NM_019677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143153.1, BC058710.1, BU846788.1 and AL935278.7. On Mar 13, 2009 this sequence version replaced NM_019677.1. Transcript Variant: This variant (2) uses an alternate 3' exon that contains an in-frame stop codon compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.35417.1, SRR1660817.52966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT TGTTACTCCA 
ATTATTTTGA GGACTGATCC TCAGGGATTT TTCTTTTACT GGACAGACCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTGCG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGACCAGAC CTGGTGAACA TTTCCCACTT GAATCTTGTG 
GCTTTTCAAG AGGAAGTAGC CAAGGAATGG ACAAATGAGG TTTTCAGCTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTCCAGGTCA CCCCAGAAGG CCGCATTCCT CTTAAAAACA TCTATCGATT GTTCTCAGCA 
GACCGGAAGC GCGTTGAAAC TGCACTAGAG GCTTGTAGTC TTCCATCTTC GAGGAATGAC 
TCCATTCCCC AAGAGGACTT CACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTCTGT 
CCCCGACCTG AAATTGATAA CATCTTTTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTG 
ACGGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTCAATGAA 
ATCCTTTACC CACCTCTGAA GCAGGAGCAG GTCCAAGTGT TGATTGAGAA ATATGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCG CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG AAGGACCGCT GAGGAAGAGC CTGTCATTAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACGTCTC CTTTCCCCAT CCTCCTTTCC TTTGAGAACC ACGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT GATGGAACCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCG CACAAGTCGT CAGAGGGAAG TGGTAAGAAG 
AAGCTTTCAG AGCAAGCTTC CAACACCTAC AGCGACTCCT CCAGTGTGTT CGAGCCTTCG 
TCTCCAGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA CGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACAGCT GGCAGCGAAG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAT TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTTGA ACAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATACAAC AAGATGCAAC TTAGCAGGAT ATATCCCAAG 
GGAACACGTG TGGATTCATC CAACTACATG CCTCAACTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAT 
GAGTACAATG GGAAGAGCGG CTATAGGCTG AAGCCAGAGT TCATGAGGAG GCCGGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TCTATCTGTT 
AAGATTATTT CAGGTCAGTT CCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGCT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ACCCTGTCTG GGAAGAAGAA CCTATTGTAT TCAAAAAGGT AGTTCTACCT 
TCTCTGGCCT GTTTGAGGAT AGCAGCATAT GAAGAGGGAG GCAAATTTAT TGGCCACCGG 
ATATTGCCTG TGCAGGCTAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGACGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA TTATGTGCCA 
GACACGTATG CAGATGTAAT CGAAGCTCTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGCT GGCTGCGTTG ACCCTGGAGG ATGAAGAGGA AGTAAAGAAG 
GAGGCTGACC CTGGAGAAAC ATCTTCCGAG GCTCCAAGCG AAACCAGGAC AACGCCAGCA 
GAGAATGGGG TGAATCACAC CGCATCCCTT GCACCCAAGC CGCCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCTGCCAAAA CAGAAGATCT GATCCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAAACCATC GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAACACTA CAAAGAAATG AAAGACCTGG TGAAGAGACA CCACAAGAAA 
ACCACGGAGC TCATTAAGGA GCACACGACC AAGTACAACG AGATTCAGAA TGACTACCTG 
AGAAGGAGGG CAGCCTTGGA GAAGTCCGCC AAAAAGGACA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCTG ACCACGGCTC ATCAGCCATT GAGCAAGACC TCGCTGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGCGA GAAGTACCAA AAGCGAGAAC ACATTAAACT GCTTATTCAG 
AAGTTGACAG ATGTTGCAGA AGAGTGTCAG AACAATCAGT TAAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA AGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCGTACATCC AGGAGGTGGT TCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TCGTGGAGAA ACACAATGAG ATCCGCCAGC AGATCCTCGA TGAGAAGCCC 
AAGGGGGAAG GCCCCTCCTC AGTCTTGTCG GAAGGTTGCC ATGAGGATCC CTCTGTTCCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062651.2
CDS	370..3891
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHNEIRQQILDEKPKGEGPSSVLSEGCHEDPSVPPNFTPPNPQALKW

Target ORF information:

RefSeq Version	NM_019677.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019677.2

ORF Insert Sequence:

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT TGTTACTCCA 
ATTATTTTGA GGACTGATCC TCAGGGATTT TTCTTTTACT GGACAGACCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTGCG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGACCAGAC CTGGTGAACA TTTCCCACTT GAATCTTGTG 
GCTTTTCAAG AGGAAGTAGC CAAGGAATGG ACAAATGAGG TTTTCAGCTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTCCAGGTCA CCCCAGAAGG CCGCATTCCT CTTAAAAACA TCTATCGATT GTTCTCAGCA 
GACCGGAAGC GCGTTGAAAC TGCACTAGAG GCTTGTAGTC TTCCATCTTC GAGGAATGAC 
TCCATTCCCC AAGAGGACTT CACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTCTGT 
CCCCGACCTG AAATTGATAA CATCTTTTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTG 
ACGGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTCAATGAA 
ATCCTTTACC CACCTCTGAA GCAGGAGCAG GTCCAAGTGT TGATTGAGAA ATATGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCG CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG AAGGACCGCT GAGGAAGAGC CTGTCATTAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACGTCTC CTTTCCCCAT CCTCCTTTCC TTTGAGAACC ACGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT GATGGAACCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCG CACAAGTCGT CAGAGGGAAG TGGTAAGAAG 
AAGCTTTCAG AGCAAGCTTC CAACACCTAC AGCGACTCCT CCAGTGTGTT CGAGCCTTCG 
TCTCCAGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA CGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACAGCT GGCAGCGAAG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAT TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTTGA ACAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATACAAC AAGATGCAAC TTAGCAGGAT ATATCCCAAG 
GGAACACGTG TGGATTCATC CAACTACATG CCTCAACTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAT 
GAGTACAATG GGAAGAGCGG CTATAGGCTG AAGCCAGAGT TCATGAGGAG GCCGGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TCTATCTGTT 
AAGATTATTT CAGGTCAGTT CCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGCT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ACCCTGTCTG GGAAGAAGAA CCTATTGTAT TCAAAAAGGT AGTTCTACCT 
TCTCTGGCCT GTTTGAGGAT AGCAGCATAT GAAGAGGGAG GCAAATTTAT TGGCCACCGG 
ATATTGCCTG TGCAGGCTAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGACGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA TTATGTGCCA 
GACACGTATG CAGATGTAAT CGAAGCTCTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGCT GGCTGCGTTG ACCCTGGAGG ATGAAGAGGA AGTAAAGAAG 
GAGGCTGACC CTGGAGAAAC ATCTTCCGAG GCTCCAAGCG AAACCAGGAC AACGCCAGCA 
GAGAATGGGG TGAATCACAC CGCATCCCTT GCACCCAAGC CGCCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCTGCCAAAA CAGAAGATCT GATCCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAAACCATC GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAACACTA CAAAGAAATG AAAGACCTGG TGAAGAGACA CCACAAGAAA 
ACCACGGAGC TCATTAAGGA GCACACGACC AAGTACAACG AGATTCAGAA TGACTACCTG 
AGAAGGAGGG CAGCCTTGGA GAAGTCCGCC AAAAAGGACA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCTG ACCACGGCTC ATCAGCCATT GAGCAAGACC TCGCTGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGCGA GAAGTACCAA AAGCGAGAAC ACATTAAACT GCTTATTCAG 
AAGTTGACAG ATGTTGCAGA AGAGTGTCAG AACAATCAGT TAAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA AGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCGTACATCC AGGAGGTGGT TCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TCGTGGAGAA ACACAATGAG ATCCGCCAGC AGATCCTCGA TGAGAAGCCC 
AAGGGGGAAG GCCCCTCCTC AGTCTTGTCG GAAGGTTGCC ATGAGGATCC CTCTGTTCCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb1 ( XM_006498930.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498993.1 Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant X1, mRNA.

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CloneID	OMu30113
Clone ID Related Accession (Same CDS sequence)	XM_006498930.2 , XM_006498930.3
Accession Version	XM_006498930.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACACA TTAATGAGGA GACGGAGCTG TTAGATCTCA GCCTTGTCAA GGATGCCAGG 
TGTGGGAAGC ACGCCAAAGC TCCCAAGGAC CCCAAGTTGC GTGAACTTCT GGATGTTGGG 
AACATCGGAC ACTTGGAACA GCGCATGATA ACTGTGGTGT ATGGACCAGA CCTGGTGAAC 
ATTTCCCACT TGAATCTTGT GGCTTTTCAA GAGGAAGTAG CCAAGGAATG GACAAATGAG 
GTTTTCAGCT TGGCAACAAA CCTGCTGGCT CAGAACATGT CCAGGGACGC ATTTCTGGAG 
AAAGCATATA CTAAGCTCAA GCTCCAGGTC ACCCCAGAAG GCCGCATTCC TCTTAAAAAC 
ATCTATCGAT TGTTCTCAGC AGACCGGAAG CGCGTTGAAA CTGCACTAGA GGCTTGTAGT 
CTTCCATCTT CGAGGAATGA CTCCATTCCC CAAGAGGACT TCACTCCAGA TGTATACAGA 
GTTTTCCTGA ACAATCTCTG TCCCCGACCT GAAATTGATA ACATCTTTTC TGAATTTGGT 
GCCAAAAGCA AACCGTACCT GACGGTTGAT CAGATGATGG ATTTTATCAA CCTTAAGCAG 
AGAGATCCCC GGCTCAATGA AATCCTTTAC CCACCTCTGA AGCAGGAGCA GGTCCAAGTG 
TTGATTGAGA AATATGAGCC CAACAGCAGC CTCGCCAAGA AAGGGCAGAT GTCAGTGGAT 
GGATTCATGC GCTACCTGAG CGGAGAAGAA AATGGAGTCG TTTCACCTGA GAAACTGGAT 
TTGAACGAAG ACATGTCTCA GCCCCTGTCT CACTATTTCA TCAATTCCTC GCACAACACC 
TACCTCACAG CTGGCCAGTT GGCTGGGAAC TCGTCTGTAG AGATGTATCG CCAGGTGCTT 
CTGTCTGGAT GTCGCTGTGT GGAGCTGGAC TGCTGGAAGG GAAGGACCGC TGAGGAAGAG 
CCTGTCATTA CCCATGGATT CACCATGACA ACAGAAATAT CCTTCAAGGA AGTCATAGAA 
GCCATCGCAG AGTGTGCGTT CAAGACGTCT CCTTTCCCCA TCCTCCTTTC CTTTGAGAAC 
CACGTGGATT CCCCGAAGCA ACAAGCCAAG ATGGCCGAGT ATTGCCGATT AATCTTTGGT 
GATGCCCTCC TGATGGAACC ACTGGAAAAA TACCCACTGG AATCTGGGGT ACCTCTTCCA 
AGCCCTATGG ATTTAATGTA TAAAATCTTG GTGAAAAACA AGAAGAAGTC GCACAAGTCG 
TCAGAGGGAA GTGGTAAGAA GAAGCTTTCA GAGCAAGCTT CCAACACCTA CAGCGACTCC 
TCCAGTGTGT TCGAGCCTTC GTCTCCAGGA GCTGGGGAAG CAGATACGGA GAGTGATGAC 
GATGACGACG ACGATGACTG TAAAAAGTCT TCCATGGATG AGGGGACAGC TGGCAGCGAA 
GCCATGGCCA CAGAAGAGAT GTCTAACCTG GTGAACTATA TTCAGCCTGT CAAGTTTGAG 
TCCTTTGAAA TTTCAAAAAA AAGAAATAAA AGCTTTGAAA TGTCTTCCTT CGTGGAAACC 
AAAGGACTTG AACAACTTAC AAAGTCTCCG GTGGAATTTG TAGAATACAA CAAGATGCAA 
CTTAGCAGGA TATATCCCAA GGGAACACGT GTGGATTCAT CCAACTACAT GCCTCAACTC 
TTCTGGAATG CTGGCTGTCA GATGGTGGCG CTCAACTTCC AGACAGTGGA TCTAGCTATG 
CAGATAAACA TGGGCATGTA TGAGTACAAT GGGAAGAGCG GCTATAGGCT GAAGCCAGAG 
TTCATGAGGA GGCCGGACAA GCATTTTGAT CCATTTACTG AAGGAATCGT AGATGGGATA 
GTGGCCAACA CTCTATCTGT TAAGATTATT TCAGGTCAGT TCCTCTCTGA TAAGAAAGTT 
GGGACTTATG TGGAAGTGGA TATGTTTGGC TTGCCTGTGG ACACAAGGAG GAAGGCATTT 
AAAACCAAGA CATCCCAAGG AAATGCTGTA AACCCTGTCT GGGAAGAAGA ACCTATTGTA 
TTCAAAAAGG TAGTTCTACC TTCTCTGGCC TGTTTGAGGA TAGCAGCATA TGAAGAGGGA 
GGCAAATTTA TTGGCCACCG GATATTGCCT GTGCAGGCTA TTCGGCCAGG CTATCACTAC 
ATCTGCCTGC GGAATGAGAG GAACCAGCCC CTGACGCTGC CAGCTGTCTT TGTCTACATA 
GAAGTCAAAG ATTATGTGCC AGACACGTAT GCAGATGTAA TCGAAGCTCT ATCAAACCCA 
ATCCGATATG TCAATCTGAT GGAACAGAGA GCTAAGCAGC TGGCTGCGTT GACCCTGGAG 
GATGAAGAGG AAGTAAAGAA GGAGGCTGAC CCTGGAGAAA CATCTTCCGA GGCTCCAAGC 
GAAACCAGGA CAACGCCAGC AGAGAATGGG GTGAATCACA CCGCATCCCT TGCACCCAAG 
CCGCCTTCCC AGGCTCCACA CAGCCAGCCT GCTCCAGGGT CTGTGAAGGC ACCTGCCAAA 
ACAGAAGATC TGATCCAGAG CGTGTTAACA GAAGTAGAGG CGCAAACCAT CGAAGAGCTC 
AAGCAACAGA AATCGTTCGT GAAACTTCAA AAGAAACACT ACAAAGAAAT GAAAGACCTG 
GTGAAGAGAC ACCACAAGAA AACCACGGAG CTCATTAAGG AGCACACGAC CAAGTACAAC 
GAGATTCAGA ATGACTACCT GAGAAGGAGG GCAGCCTTGG AGAAGTCCGC CAAAAAGGAC 
AGCAAGAAGA AATCTGAACC CAGCAGCCCT GACCACGGCT CATCAGCCAT TGAGCAAGAC 
CTCGCTGCCC TGGATGCAGA AATGACTCAG AAGTTGATAG ACTTGAAAGA CAAGCAACAA 
CAGCAGCTGC TTAATCTTCG GCAAGAGCAG TATTACAGCG AGAAGTACCA AAAGCGAGAA 
CACATTAAAC TGCTTATTCA GAAGTTGACA GATGTTGCAG AAGAGTGTCA GAACAATCAG 
TTAAAGAAGC TGAAGGAAAT CTGCGAGAAA GAGAAGAAGG AATTAAAGAA GAAAATGGAT 
AAGAAGAGGC AAGAGAAGAT AACAGAAGCC AAGTCCAAAG ACAAAAGCCA GATGGAAGAG 
GAGAAGACAG AGATGATCCG ATCGTACATC CAGGAGGTGG TTCAGTACAT CAAGAGGTTA 
GAGGAAGCAC AAAGTAAAAG ACAAGAAAAA CTCGTGGAGA AACACAATGA GATCCGCCAG 
CAGATCCTCG ATGAGAAGCC CAAGCTGCAG ACGGAGCTGG AGCAAGAATA CCAAGACAAG 
TTCAAAAGGC TGCCCCTGGA GATTCTGGAG TTTGTACAGG AAGCCATGAA AGGGAAGATC 
AGCGAGGACA GCAATCACGG CTCTGCCCCT CCCTCTCTGG CCTCAGATGC TGCAAAGGTG 
AACCTCAAGT CTCCCTCCAG TGAGGAGATA GAAAGAGAGA ACCCAGGAAG AGAGTTTGAT 
ACTCCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498993.1
CDS	5441..8932
Translation	MKHINEETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHNEIRQQILDEKPKLQTELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPPSLASDAAKVNLKSPSSEEIERENPGREFDTPL

Target ORF information:

RefSeq Version	XM_006498930.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498930.2

ORF Insert Sequence:

ATGAAACACA TTAATGAGGA GACGGAGCTG TTAGATCTCA GCCTTGTCAA GGATGCCAGG 
TGTGGGAAGC ACGCCAAAGC TCCCAAGGAC CCCAAGTTGC GTGAACTTCT GGATGTTGGG 
AACATCGGAC ACTTGGAACA GCGCATGATA ACTGTGGTGT ATGGACCAGA CCTGGTGAAC 
ATTTCCCACT TGAATCTTGT GGCTTTTCAA GAGGAAGTAG CCAAGGAATG GACAAATGAG 
GTTTTCAGCT TGGCAACAAA CCTGCTGGCT CAGAACATGT CCAGGGACGC ATTTCTGGAG 
AAAGCATATA CTAAGCTCAA GCTCCAGGTC ACCCCAGAAG GCCGCATTCC TCTTAAAAAC 
ATCTATCGAT TGTTCTCAGC AGACCGGAAG CGCGTTGAAA CTGCACTAGA GGCTTGTAGT 
CTTCCATCTT CGAGGAATGA CTCCATTCCC CAAGAGGACT TCACTCCAGA TGTATACAGA 
GTTTTCCTGA ACAATCTCTG TCCCCGACCT GAAATTGATA ACATCTTTTC TGAATTTGGT 
GCCAAAAGCA AACCGTACCT GACGGTTGAT CAGATGATGG ATTTTATCAA CCTTAAGCAG 
AGAGATCCCC GGCTCAATGA AATCCTTTAC CCACCTCTGA AGCAGGAGCA GGTCCAAGTG 
TTGATTGAGA AATATGAGCC CAACAGCAGC CTCGCCAAGA AAGGGCAGAT GTCAGTGGAT 
GGATTCATGC GCTACCTGAG CGGAGAAGAA AATGGAGTCG TTTCACCTGA GAAACTGGAT 
TTGAACGAAG ACATGTCTCA GCCCCTGTCT CACTATTTCA TCAATTCCTC GCACAACACC 
TACCTCACAG CTGGCCAGTT GGCTGGGAAC TCGTCTGTAG AGATGTATCG CCAGGTGCTT 
CTGTCTGGAT GTCGCTGTGT GGAGCTGGAC TGCTGGAAGG GAAGGACCGC TGAGGAAGAG 
CCTGTCATTA CCCATGGATT CACCATGACA ACAGAAATAT CCTTCAAGGA AGTCATAGAA 
GCCATCGCAG AGTGTGCGTT CAAGACGTCT CCTTTCCCCA TCCTCCTTTC CTTTGAGAAC 
CACGTGGATT CCCCGAAGCA ACAAGCCAAG ATGGCCGAGT ATTGCCGATT AATCTTTGGT 
GATGCCCTCC TGATGGAACC ACTGGAAAAA TACCCACTGG AATCTGGGGT ACCTCTTCCA 
AGCCCTATGG ATTTAATGTA TAAAATCTTG GTGAAAAACA AGAAGAAGTC GCACAAGTCG 
TCAGAGGGAA GTGGTAAGAA GAAGCTTTCA GAGCAAGCTT CCAACACCTA CAGCGACTCC 
TCCAGTGTGT TCGAGCCTTC GTCTCCAGGA GCTGGGGAAG CAGATACGGA GAGTGATGAC 
GATGACGACG ACGATGACTG TAAAAAGTCT TCCATGGATG AGGGGACAGC TGGCAGCGAA 
GCCATGGCCA CAGAAGAGAT GTCTAACCTG GTGAACTATA TTCAGCCTGT CAAGTTTGAG 
TCCTTTGAAA TTTCAAAAAA AAGAAATAAA AGCTTTGAAA TGTCTTCCTT CGTGGAAACC 
AAAGGACTTG AACAACTTAC AAAGTCTCCG GTGGAATTTG TAGAATACAA CAAGATGCAA 
CTTAGCAGGA TATATCCCAA GGGAACACGT GTGGATTCAT CCAACTACAT GCCTCAACTC 
TTCTGGAATG CTGGCTGTCA GATGGTGGCG CTCAACTTCC AGACAGTGGA TCTAGCTATG 
CAGATAAACA TGGGCATGTA TGAGTACAAT GGGAAGAGCG GCTATAGGCT GAAGCCAGAG 
TTCATGAGGA GGCCGGACAA GCATTTTGAT CCATTTACTG AAGGAATCGT AGATGGGATA 
GTGGCCAACA CTCTATCTGT TAAGATTATT TCAGGTCAGT TCCTCTCTGA TAAGAAAGTT 
GGGACTTATG TGGAAGTGGA TATGTTTGGC TTGCCTGTGG ACACAAGGAG GAAGGCATTT 
AAAACCAAGA CATCCCAAGG AAATGCTGTA AACCCTGTCT GGGAAGAAGA ACCTATTGTA 
TTCAAAAAGG TAGTTCTACC TTCTCTGGCC TGTTTGAGGA TAGCAGCATA TGAAGAGGGA 
GGCAAATTTA TTGGCCACCG GATATTGCCT GTGCAGGCTA TTCGGCCAGG CTATCACTAC 
ATCTGCCTGC GGAATGAGAG GAACCAGCCC CTGACGCTGC CAGCTGTCTT TGTCTACATA 
GAAGTCAAAG ATTATGTGCC AGACACGTAT GCAGATGTAA TCGAAGCTCT ATCAAACCCA 
ATCCGATATG TCAATCTGAT GGAACAGAGA GCTAAGCAGC TGGCTGCGTT GACCCTGGAG 
GATGAAGAGG AAGTAAAGAA GGAGGCTGAC CCTGGAGAAA CATCTTCCGA GGCTCCAAGC 
GAAACCAGGA CAACGCCAGC AGAGAATGGG GTGAATCACA CCGCATCCCT TGCACCCAAG 
CCGCCTTCCC AGGCTCCACA CAGCCAGCCT GCTCCAGGGT CTGTGAAGGC ACCTGCCAAA 
ACAGAAGATC TGATCCAGAG CGTGTTAACA GAAGTAGAGG CGCAAACCAT CGAAGAGCTC 
AAGCAACAGA AATCGTTCGT GAAACTTCAA AAGAAACACT ACAAAGAAAT GAAAGACCTG 
GTGAAGAGAC ACCACAAGAA AACCACGGAG CTCATTAAGG AGCACACGAC CAAGTACAAC 
GAGATTCAGA ATGACTACCT GAGAAGGAGG GCAGCCTTGG AGAAGTCCGC CAAAAAGGAC 
AGCAAGAAGA AATCTGAACC CAGCAGCCCT GACCACGGCT CATCAGCCAT TGAGCAAGAC 
CTCGCTGCCC TGGATGCAGA AATGACTCAG AAGTTGATAG ACTTGAAAGA CAAGCAACAA 
CAGCAGCTGC TTAATCTTCG GCAAGAGCAG TATTACAGCG AGAAGTACCA AAAGCGAGAA 
CACATTAAAC TGCTTATTCA GAAGTTGACA GATGTTGCAG AAGAGTGTCA GAACAATCAG 
TTAAAGAAGC TGAAGGAAAT CTGCGAGAAA GAGAAGAAGG AATTAAAGAA GAAAATGGAT 
AAGAAGAGGC AAGAGAAGAT AACAGAAGCC AAGTCCAAAG ACAAAAGCCA GATGGAAGAG 
GAGAAGACAG AGATGATCCG ATCGTACATC CAGGAGGTGG TTCAGTACAT CAAGAGGTTA 
GAGGAAGCAC AAAGTAAAAG ACAAGAAAAA CTCGTGGAGA AACACAATGA GATCCGCCAG 
CAGATCCTCG ATGAGAAGCC CAAGCTGCAG ACGGAGCTGG AGCAAGAATA CCAAGACAAG 
TTCAAAAGGC TGCCCCTGGA GATTCTGGAG TTTGTACAGG AAGCCATGAA AGGGAAGATC 
AGCGAGGACA GCAATCACGG CTCTGCCCCT CCCTCTCTGG CCTCAGATGC TGCAAAGGTG 
AACCTCAAGT CTCCCTCCAG TGAGGAGATA GAAAGAGAGA ACCCAGGAAG AGAGTTTGAT 
ACTCCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synergistic Signaling by Light and Acetylcholine in Mouse Iris Sphincter Muscle.
Current biology : CB27(12)1791-1800.e5(2017 Jun)
Wang Q,Yue WWS,Jiang Z,Xue T,Kang SH,Bergles DE,Mikoshiba K,Offermanns S,Yau KW

PLC-?1 and cell differentiation: An insight into myogenesis and osteogenesis.
Advances in biological regulation631-5(2017 Jan)
Ramazzotti G,Faenza I,Fiume R,Billi AM,Manzoli L,Mongiorgi S,Ratti S,McCubrey JA,Suh PG,Cocco L,Follo MY

IPMK and ?-catenin mediate PLC-?1-dependent signaling in myogenic differentiation.
Oncotarget7(51)84118-84127(2016 Dec)
Ramazzotti G,Billi AM,Manzoli L,Mazzetti C,Ruggeri A,Erneux C,Kim S,Suh PG,Cocco L,Faenza I

Phospholipase C?1 induces membrane tubulation and is involved in caveolae formation.
Proceedings of the National Academy of Sciences of the United States of America113(28)7834-9(2016 07)
Inaba T,Kishimoto T,Murate M,Tajima T,Sakai S,Abe M,Makino A,Tomishige N,Ishitsuka R,Ikeda Y,Takeoka S,Kobayashi T

MLP and CARP are linked to chronic PKC? signalling in dilated cardiomyopathy.
Nature communications712120(2016 Jun)
Lange S,Gehmlich K,Lun AS,Blondelle J,Hooper C,Dalton ND,Alvarez EA,Zhang X,Bang ML,Abassi YA,Dos Remedios CG,Peterson KL,Chen J,Ehler E

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Plcb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

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FAX

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