Prkcq cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkcq
Entrez Gene ID	18761
Full Name	protein kinase C, theta
Synonyms	A130035A12Rik,AW494342,PKC-0,PKC-theta,PKCtheta,Pkcq
General protein information	Preferred Names protein kinase C, theta Names protein kinase C theta type PKC theta nPKC-theta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 A1\|2 8.42 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030248462.1	XP_030104322.1	protein kinase C theta type isoform X2
XM_006497399.2	XP_006497462.1	protein kinase C theta type isoform X3
XM_006497398.3	XP_006497461.1	protein kinase C theta type isoform X1
XM_011238965.2	XP_011237267.1	protein kinase C theta type isoform X2
NM_008859.2	NP_032885.1	protein kinase C theta type
XM_006497398.4	XP_006497461.1	protein kinase C theta type isoform X1
XM_030248463.1	XP_030104323.1	protein kinase C theta type isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKCQ	NP_006248.1
Pan troglodytes (chimpanzee)	PRKCQ	XP_001148074.1
Canis lupus familiaris (dog)	PRKCQ	XP_005617224.1
Gallus gallus (chicken)	PRKCQ	XP_001235980.2
	PRKCQ	XP_001118689.2
Rattus norvegicus (Norway rat)	Prkcq	NP_001263650.1
Danio rerio (zebrafish)	prkcq	NP_001082839.2
Bos taurus (cattle)	PRKCQ	NP_001179006.1
Mus musculus (house mouse)	Prkcq	NP_032885.1

Gene Ontology
Bibliography

Related articles in PubMed

SRC1 promotes Th17 differentiation by overriding Foxp3 suppression to stimulate ROR?t activity in a PKC-?-dependent manner.
Sen S, Wang F, Zhang J, He Z, Ma J, Gwack Y, Xu J, Sun Z
Proceedings of the National Academy of Sciences of the United States of America115(3)E458-E467(2018 Jan)

Targeting early PKC?-dependent T-cell infiltration of dystrophic muscle reduces disease severity in a mouse model of muscular dystrophy.
Lozanoska-Ochser B, Benedetti A, Rizzo G, Marrocco V, Di Maggio R, Fiore P, Bouche M
The Journal of pathology(2017 Dec)

Mice Lacking PKC? in Skeletal Muscle Have Reduced Intramyocellular Lipid Accumulation and Increased Insulin Responsiveness in Skeletal Muscle.
Peck B, Huot J, Renzi T, Arthur ST, Turner MJ, Marino JS
American journal of physiology. Regulatory, integrative and comparative physiologyajpregu005212016(2017 Nov)

PKC-? is dispensable for OX40L-induced TCR-independent Treg proliferation but contributes by enabling IL-2 production from effector T-cells.
Alharshawi K, Marinelarena A, Kumar P, El-Sayed O, Bhattacharya P, Sun Z, Epstein AL, Maker AV, Prabhakar BS
Scientific reports7(1)6594(2017 Jul)

Human in vitro-induced regulatory T cells display Dlgh1dependent and PKC-? restrained suppressive activity.
Zanin-Zhorov A, Kumari S, Hippen KL, Merkel SC, MacMillan ML, Blazar BR, Dustin ML
Scientific reports7(1)4258(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PKC-theta; is a critical factor for type 2 innate lymphoid cells activation that contributes to TH2 cell differentiation, which is associated with IRF4 and NFAT1 expression in allergic lung inflammation.
Title: Protein kinase C? controls type 2 innate lymphoid cell and T

Data show that pharmacological inhibition of Protein Kinase C theta (PKCtheta;) in Duchenne Muscular Dystrophy (DMD) can be considered an attractive strategy to modulate immune response and prevent the progression of the disease.
Title: Pharmacological Inhibition of PKC? Counteracts Muscle Disease in a Mouse Model of Duchenne Muscular Dystrophy.

Results suggest that while PKCtheta; is involved in Treg cell differentiation in vivo, it is dispensable for Treg-mediated suppression.
Title: Protein kinase C theta is dispensable for suppression mediated by CD25+CD4+ regulatory T cells.

PKCtheta; selectively inactivates the negative regulator of F-actin generation, Coronin 1A, at the center of the T cell interface with the antigen presenting cell (APC). This allows for effective generation of the large actin-based lamellum required for recruitment of the Notch-processing membrane metalloproteinase ADAM10.
Title: PKC? links proximal T cell and Notch signaling through localized regulation of the actin cytoskeleton.

Sumoylation of PKC-theta; was essential for T cell activation. Desumoylation did not affect the catalytic activity of PKC-theta; but inhibited the association of CD28 with PKC-theta; and filamin A and impaired the assembly of a mature immunological synapse.
Title: TCR-induced sumoylation of the kinase PKC-? controls T cell synapse organization and T cell activation.

The following Prkcq gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkcq cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu29095	XM_006497399.2 Latest version!	Mus musculus protein kinase C, theta (Prkcq), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu68442	XM_011238965.2 Latest version!	Mus musculus protein kinase C, theta (Prkcq), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19611	NM_008859.2 Latest version!	Mus musculus protein kinase C, theta (Prkcq), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu29094	XM_006497398.4 Latest version!	Mus musculus protein kinase C, theta (Prkcq), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu68442	XM_030248463.1 Latest version!	Mus musculus protein kinase C, theta (Prkcq), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu68442	XM_030248462.1 Latest version!	Mus musculus protein kinase C, theta (Prkcq), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu29094	XM_006497398.3	Mus musculus protein kinase C, theta (Prkcq), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkcq ( XM_006497398.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497461.1 Mus musculus protein kinase C, theta (Prkcq), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu29094
Clone ID Related Accession (Same CDS sequence)	XM_006497398.3 , XM_006497398.4
Accession Version	XM_006497398.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein kinase C theta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCC AGAAAAAGCC AACCATGTAC CCACCTTGGG ACAGCACCTT TGACGCCCAC 
ATTAACAAGG GAAGGGTGAT GCAGATCATC GTGAAGGGCA AGAATGTAGA CCTCATCTCA 
GAAACAACCG TGGAACTCTA CTCCCTGGCG GAGAGATGCC GCAAGAACAA TGGGCGGACA 
GAAATATGGT TAGAGCTGAA ACCTCAAGGC CGAATGCTAA TGAATGCAAG ATACTTTCTG 
GAAATGAGTG ACACAAAGGA CATGAGTGAG TTTGAGAATG AAGGATTCTT TGCACTGCAT 
CAGCGCCGAG GAGCCATCAA ACAGGCCAAA GTCCACCATG TCAAGTGTCA CGAGTTCACG 
GCCACCTTTT TCCCTCAACC CACATTTTGC TCTGTCTGCC ATGAATTTGT CTGGGGCCTG 
AACAAGCAGG GTTACCAGTG CCGACAGTGT AATGCAGCGA TTCACAAGAA GTGCATTGAT 
AAAGTGATAG CCAAGTGCAC AGGATCCGCA ATCAATAGCC GAGAAACCAT GTTCCATAAG 
GAGAGATTCA AGATCGACAT GCCACACAGA TTCAAAGTCT ACAACTACAA GAGTCCAACC 
TTCTGTGAGC ACTGTGGTAC CCTGCTCTGG GGGCTGGCGA GGCAAGGACT CAAATGTGAT 
GCATGTGGCA TGAACGTCCA CCACCGATGC CAGACAAAGG TTGCCAATCT TTGTGGTATA 
AACCAGAAGC TAATGGCTGA AGCACTAGCG ATGATTGAAA GCACCCAACA GGCTCGCTCC 
TTACGAGATT CAGAACACAT CTTCCGAGAA GGCCCAGTTG AAATTGGTCT CCCATGCTCC 
ACCAAAAACG AAACCAGGCC ACCATGCGTA CCAACACCTG GGAAAAGAGA ACCCCAGGGC 
ATTTCCTGGG ATTCCCCTTT GGATGGGTCA AATAAATCGG CCGGTCCTCC TGAACCCGAA 
GTGAGCATGC GCAGGACTTC ACTGCAGCTG AAACTGAAGA TCGATGACTT CATCCTGCAC 
AAGATGTTGG GAAAAGGAAG TTTTGGCAAG GTCTTCCTGG CAGAGTTCAA GAGAACCAAT 
CAGTTTTTCG CAATAAAAGC CTTAAAGAAA GATGTGGTGT TGATGGATGA TGACGTCGAG 
TGTACAATGG TGGAAAAGAG GGTTCTGTCC TTGGCATGGG AGCATCCATT TCTAACACAC 
ATGTTCTGCA CATTCCAGAC CAAGGAAAAT CTCTTTTTCG TGATGGAGTA TCTCAATGGA 
GGCGACTTAA TGTACCACAT CCAAAGTTGC CACAAATTTG ATCTTTCCAG AGCCACGTTT 
TATGCTGCTG AGGTCATCCT TGGTCTGCAG TTCCTTCATT CCAAAGGAAT TGTCTACAGG 
GACCTGAAGC TTGATAATAT CCTGTTAGAC AGAGATGGAC ATATCAAAAT AGCAGACTTT 
GGGATGTGCA AAGAGAACAT GCTAGGAGAT GCGAAGACAA ATACTTTCTG TGGAACTCCT 
GACTACATTG CTCCGGAGAT CTTGCTGGGT CAGAAGTACA ACCATTCCGT CGACTGGTGG 
TCCTTCGGGG TGCTCGTTTA TGAGATGCTG ATTGGCCAGT CCCCCTTCCA CGGGCAGGAC 
GAGGAGGAGC TGTTCCACTC CATCCGCATG GACAACCCCT TCTACCCGAG GTGGCTCGAA 
AGGGAGGCCA AGGACCTTCT AGTGAAGCTT TTTGTGAGAG AACCTGAGAA GAGGCTGGGA 
GTGAGAGGAG ACATCCGCCA GCATCCTTTG TTTCGAGAGA TCAACTGGGA AGAGCTTGAA 
AGGAAAGAGA TTGACCCACC CTTCAGACCA AAAGTGAAAT CACCATATGA CTGTAGCAAT 
TTCGACAAGG AATTCCTAAG TGAGAAACCC CGGCTATCAT TCGCCGACAG AGCACTCATC 
AACAGCATGG ACCAGAACAT GTTCAGCAAC TTTTCCTTCA TTAACCCAGG GATGGAGACT 
CTCATTTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497461.1
CDS	569..2563
Translation	MYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGRTEIWLELKPQGRMLMNARYFLEMSDTKDMSEFENEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVPTPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS

Target ORF information:

RefSeq Version	XM_006497398.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, theta (Prkcq), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497398.3

ORF Insert Sequence:

ATGTACATCC AGAAAAAGCC AACCATGTAC CCACCTTGGG ACAGCACCTT TGACGCCCAC 
ATTAACAAGG GAAGGGTGAT GCAGATCATC GTGAAGGGCA AGAATGTAGA CCTCATCTCA 
GAAACAACCG TGGAACTCTA CTCCCTGGCG GAGAGATGCC GCAAGAACAA TGGGCGGACA 
GAAATATGGT TAGAGCTGAA ACCTCAAGGC CGAATGCTAA TGAATGCAAG ATACTTTCTG 
GAAATGAGTG ACACAAAGGA CATGAGTGAG TTTGAGAATG AAGGATTCTT TGCACTGCAT 
CAGCGCCGAG GAGCCATCAA ACAGGCCAAA GTCCACCATG TCAAGTGTCA CGAGTTCACG 
GCCACCTTTT TCCCTCAACC CACATTTTGC TCTGTCTGCC ATGAATTTGT CTGGGGCCTG 
AACAAGCAGG GTTACCAGTG CCGACAGTGT AATGCAGCGA TTCACAAGAA GTGCATTGAT 
AAAGTGATAG CCAAGTGCAC AGGATCCGCA ATCAATAGCC GAGAAACCAT GTTCCATAAG 
GAGAGATTCA AGATCGACAT GCCACACAGA TTCAAAGTCT ACAACTACAA GAGTCCAACC 
TTCTGTGAGC ACTGTGGTAC CCTGCTCTGG GGGCTGGCGA GGCAAGGACT CAAATGTGAT 
GCATGTGGCA TGAACGTCCA CCACCGATGC CAGACAAAGG TTGCCAATCT TTGTGGTATA 
AACCAGAAGC TAATGGCTGA AGCACTAGCG ATGATTGAAA GCACCCAACA GGCTCGCTCC 
TTACGAGATT CAGAACACAT CTTCCGAGAA GGCCCAGTTG AAATTGGTCT CCCATGCTCC 
ACCAAAAACG AAACCAGGCC ACCATGCGTA CCAACACCTG GGAAAAGAGA ACCCCAGGGC 
ATTTCCTGGG ATTCCCCTTT GGATGGGTCA AATAAATCGG CCGGTCCTCC TGAACCCGAA 
GTGAGCATGC GCAGGACTTC ACTGCAGCTG AAACTGAAGA TCGATGACTT CATCCTGCAC 
AAGATGTTGG GAAAAGGAAG TTTTGGCAAG GTCTTCCTGG CAGAGTTCAA GAGAACCAAT 
CAGTTTTTCG CAATAAAAGC CTTAAAGAAA GATGTGGTGT TGATGGATGA TGACGTCGAG 
TGTACAATGG TGGAAAAGAG GGTTCTGTCC TTGGCATGGG AGCATCCATT TCTAACACAC 
ATGTTCTGCA CATTCCAGAC CAAGGAAAAT CTCTTTTTCG TGATGGAGTA TCTCAATGGA 
GGCGACTTAA TGTACCACAT CCAAAGTTGC CACAAATTTG ATCTTTCCAG AGCCACGTTT 
TATGCTGCTG AGGTCATCCT TGGTCTGCAG TTCCTTCATT CCAAAGGAAT TGTCTACAGG 
GACCTGAAGC TTGATAATAT CCTGTTAGAC AGAGATGGAC ATATCAAAAT AGCAGACTTT 
GGGATGTGCA AAGAGAACAT GCTAGGAGAT GCGAAGACAA ATACTTTCTG TGGAACTCCT 
GACTACATTG CTCCGGAGAT CTTGCTGGGT CAGAAGTACA ACCATTCCGT CGACTGGTGG 
TCCTTCGGGG TGCTCGTTTA TGAGATGCTG ATTGGCCAGT CCCCCTTCCA CGGGCAGGAC 
GAGGAGGAGC TGTTCCACTC CATCCGCATG GACAACCCCT TCTACCCGAG GTGGCTCGAA 
AGGGAGGCCA AGGACCTTCT AGTGAAGCTT TTTGTGAGAG AACCTGAGAA GAGGCTGGGA 
GTGAGAGGAG ACATCCGCCA GCATCCTTTG TTTCGAGAGA TCAACTGGGA AGAGCTTGAA 
AGGAAAGAGA TTGACCCACC CTTCAGACCA AAAGTGAAAT CACCATATGA CTGTAGCAAT 
TTCGACAAGG AATTCCTAAG TGAGAAACCC CGGCTATCAT TCGCCGACAG AGCACTCATC 
AACAGCATGG ACCAGAACAT GTTCAGCAAC TTTTCCTTCA TTAACCCAGG GATGGAGACT 
CTCATTTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcq ( XM_006497399.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497462.1 Mus musculus protein kinase C, theta (Prkcq), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu29095
Clone ID Related Accession (Same CDS sequence)	XM_006497399.2
Accession Version	XM_006497399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kinase C theta type isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAG TCATCAGAAG GGACTATTTG CGCTCCCAGA GGTCAAGTTT TCACCTGATA 
GCATGTGGCA TGAACGTCCA CCACCGATGC CAGACAAAGG TTGCCAATCT TTGTGGTATA 
AACCAGAAGC TAATGGCTGA AGCACTAGCG ATGATTGAAA GCACCCAACA GGCTCGCTCC 
TTACGAGATT CAGAACACAT CTTCCGAGAA GGCCCAGTTG AAATTGGTCT CCCATGCTCC 
ACCAAAAACG AAACCAGGCC ACCATGCGTA CCAACACCTG GGAAAAGAGA ACCCCAGGGC 
ATTTCCTGGG ATTCCCCTTT GGATGGGTCA AATAAATCGG CCGGTCCTCC TGAACCCGAA 
GTGAGCATGC GCAGGACTTC ACTGCAGCTG AAACTGAAGA TCGATGACTT CATCCTGCAC 
AAGATGTTGG GAAAAGGAAG TTTTGGCAAG GTCTTCCTGG CAGAGTTCAA GAGAACCAAT 
CAGTTTTTCG CAATAAAAGC CTTAAAGAAA GATGTGGTGT TGATGGATGA TGACGTCGAG 
TGTACAATGG TGGAAAAGAG GGTTCTGTCC TTGGCATGGG AGCATCCATT TCTAACACAC 
ATGTTCTGCA CATTCCAGAC CAAGGAAAAT CTCTTTTTCG TGATGGAGTA TCTCAATGGA 
GGCGACTTAA TGTACCACAT CCAAAGTTGC CACAAATTTG ATCTTTCCAG AGCCACGTTT 
TATGCTGCTG AGGTCATCCT TGGTCTGCAG TTCCTTCATT CCAAAGGAAT TGTCTACAGG 
GACCTGAAGC TTGATAATAT CCTGTTAGAC AGAGATGGAC ATATCAAAAT AGCAGACTTT 
GGGATGTGCA AAGAGAACAT GCTAGGAGAT GCGAAGACAA ATACTTTCTG TGGAACTCCT 
GACTACATTG CTCCGGAGAT CTTGCTGGGT CAGAAGTACA ACCATTCCGT CGACTGGTGG 
TCCTTCGGGG TGCTCGTTTA TGAGATGCTG ATTGGCCAGT CCCCCTTCCA CGGGCAGGAC 
GAGGAGGAGC TGTTCCACTC CATCCGCATG GACAACCCCT TCTACCCGAG GTGGCTCGAA 
AGGGAGGCCA AGGACCTTCT AGTGAAGCTT TTTGTGAGAG AACCTGAGAA GAGGCTGGGA 
GTGAGAGGAG ACATCCGCCA GCATCCTTTG TTTCGAGAGA TCAACTGGGA AGAGCTTGAA 
AGGAAAGAGA TTGACCCACC CTTCAGACCA AAAGTGAAAT CACCATATGA CTGTAGCAAT 
TTCGACAAGG AATTCCTAAG TGAGAAACCC CGGCTATCAT TCGCCGACAG AGCACTCATC 
AACAGCATGG ACCAGAACAT GTTCAGCAAC TTTTCCTTCA TTAACCCAGG GATGGAGACT 
CTCATTTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497462.1
CDS	297..1691
Translation	MKIVIRRDYLRSQRSSFHLIACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVPTPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS

Target ORF information:

RefSeq Version	XM_006497399.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, theta (Prkcq), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497399.2

ORF Insert Sequence:

ATGAAAATAG TCATCAGAAG GGACTATTTG CGCTCCCAGA GGTCAAGTTT TCACCTGATA 
GCATGTGGCA TGAACGTCCA CCACCGATGC CAGACAAAGG TTGCCAATCT TTGTGGTATA 
AACCAGAAGC TAATGGCTGA AGCACTAGCG ATGATTGAAA GCACCCAACA GGCTCGCTCC 
TTACGAGATT CAGAACACAT CTTCCGAGAA GGCCCAGTTG AAATTGGTCT CCCATGCTCC 
ACCAAAAACG AAACCAGGCC ACCATGCGTA CCAACACCTG GGAAAAGAGA ACCCCAGGGC 
ATTTCCTGGG ATTCCCCTTT GGATGGGTCA AATAAATCGG CCGGTCCTCC TGAACCCGAA 
GTGAGCATGC GCAGGACTTC ACTGCAGCTG AAACTGAAGA TCGATGACTT CATCCTGCAC 
AAGATGTTGG GAAAAGGAAG TTTTGGCAAG GTCTTCCTGG CAGAGTTCAA GAGAACCAAT 
CAGTTTTTCG CAATAAAAGC CTTAAAGAAA GATGTGGTGT TGATGGATGA TGACGTCGAG 
TGTACAATGG TGGAAAAGAG GGTTCTGTCC TTGGCATGGG AGCATCCATT TCTAACACAC 
ATGTTCTGCA CATTCCAGAC CAAGGAAAAT CTCTTTTTCG TGATGGAGTA TCTCAATGGA 
GGCGACTTAA TGTACCACAT CCAAAGTTGC CACAAATTTG ATCTTTCCAG AGCCACGTTT 
TATGCTGCTG AGGTCATCCT TGGTCTGCAG TTCCTTCATT CCAAAGGAAT TGTCTACAGG 
GACCTGAAGC TTGATAATAT CCTGTTAGAC AGAGATGGAC ATATCAAAAT AGCAGACTTT 
GGGATGTGCA AAGAGAACAT GCTAGGAGAT GCGAAGACAA ATACTTTCTG TGGAACTCCT 
GACTACATTG CTCCGGAGAT CTTGCTGGGT CAGAAGTACA ACCATTCCGT CGACTGGTGG 
TCCTTCGGGG TGCTCGTTTA TGAGATGCTG ATTGGCCAGT CCCCCTTCCA CGGGCAGGAC 
GAGGAGGAGC TGTTCCACTC CATCCGCATG GACAACCCCT TCTACCCGAG GTGGCTCGAA 
AGGGAGGCCA AGGACCTTCT AGTGAAGCTT TTTGTGAGAG AACCTGAGAA GAGGCTGGGA 
GTGAGAGGAG ACATCCGCCA GCATCCTTTG TTTCGAGAGA TCAACTGGGA AGAGCTTGAA 
AGGAAAGAGA TTGACCCACC CTTCAGACCA AAAGTGAAAT CACCATATGA CTGTAGCAAT 
TTCGACAAGG AATTCCTAAG TGAGAAACCC CGGCTATCAT TCGCCGACAG AGCACTCATC 
AACAGCATGG ACCAGAACAT GTTCAGCAAC TTTTCCTTCA TTAACCCAGG GATGGAGACT 
CTCATTTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcq ( XM_011238965.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237267.1 Mus musculus protein kinase C, theta (Prkcq), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68442
Clone ID Related Accession (Same CDS sequence)	XM_030248462.1 , XM_011238965.2 , XM_030248463.1
Accession Version	XM_011238965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein kinase C theta type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAAG AACAATGGGC GGACAGAAAT ATGGACACAA AGGACATGAG TGAGTTTGAG 
AATGAAGGAT TCTTTGCACT GCATCAGCGC CGAGGAGCCA TCAAACAGGC CAAAGTCCAC 
CATGTCAAGT GTCACGAGTT CACGGCCACC TTTTTCCCTC AACCCACATT TTGCTCTGTC 
TGCCATGAAT TTGTCTGGGG CCTGAACAAG CAGGGTTACC AGTGCCGACA GTGTAATGCA 
GCGATTCACA AGAAGTGCAT TGATAAAGTG ATAGCCAAGT GCACAGGATC CGCAATCAAT 
AGCCGAGAAA CCATGTTCCA TAAGGAGAGA TTCAAGATCG ACATGCCACA CAGATTCAAA 
GTCTACAACT ACAAGAGTCC AACCTTCTGT GAGCACTGTG GTACCCTGCT CTGGGGGCTG 
GCGAGGCAAG GACTCAAATG TGATGCATGT GGCATGAACG TCCACCACCG ATGCCAGACA 
AAGGTTGCCA ATCTTTGTGG TATAAACCAG AAGCTAATGG CTGAAGCACT AGCGATGATT 
GAAAGCACCC AACAGGCTCG CTCCTTACGA GATTCAGAAC ACATCTTCCG AGAAGGCCCA 
GTTGAAATTG GTCTCCCATG CTCCACCAAA AACGAAACCA GGCCACCATG CGTACCAACA 
CCTGGGAAAA GAGAACCCCA GGGCATTTCC TGGGATTCCC CTTTGGATGG GTCAAATAAA 
TCGGCCGGTC CTCCTGAACC CGAAGTGAGC ATGCGCAGGA CTTCACTGCA GCTGAAACTG 
AAGATCGATG ACTTCATCCT GCACAAGATG TTGGGAAAAG GAAGTTTTGG CAAGGTCTTC 
CTGGCAGAGT TCAAGAGAAC CAATCAGTTT TTCGCAATAA AAGCCTTAAA GAAAGATGTG 
GTGTTGATGG ATGATGACGT CGAGTGTACA ATGGTGGAAA AGAGGGTTCT GTCCTTGGCA 
TGGGAGCATC CATTTCTAAC ACACATGTTC TGCACATTCC AGACCAAGGA AAATCTCTTT 
TTCGTGATGG AGTATCTCAA TGGAGGCGAC TTAATGTACC ACATCCAAAG TTGCCACAAA 
TTTGATCTTT CCAGAGCCAC GTTTTATGCT GCTGAGGTCA TCCTTGGTCT GCAGTTCCTT 
CATTCCAAAG GAATTGTCTA CAGGGACCTG AAGCTTGATA ATATCCTGTT AGACAGAGAT 
GGACATATCA AAATAGCAGA CTTTGGGATG TGCAAAGAGA ACATGCTAGG AGATGCGAAG 
ACAAATACTT TCTGTGGAAC TCCTGACTAC ATTGCTCCGG AGATCTTGCT GGGTCAGAAG 
TACAACCATT CCGTCGACTG GTGGTCCTTC GGGGTGCTCG TTTATGAGAT GCTGATTGGC 
CAGTCCCCCT TCCACGGGCA GGACGAGGAG GAGCTGTTCC ACTCCATCCG CATGGACAAC 
CCCTTCTACC CGAGGTGGCT CGAAAGGGAG GCCAAGGACC TTCTAGTGAA GCTTTTTGTG 
AGAGAACCTG AGAAGAGGCT GGGAGTGAGA GGAGACATCC GCCAGCATCC TTTGTTTCGA 
GAGATCAACT GGGAAGAGCT TGAAAGGAAA GAGATTGACC CACCCTTCAG ACCAAAAGTG 
AAATCACCAT ATGACTGTAG CAATTTCGAC AAGGAATTCC TAAGTGAGAA ACCCCGGCTA 
TCATTCGCCG ACAGAGCACT CATCAACAGC ATGGACCAGA ACATGTTCAG CAACTTTTCC 
TTCATTAACC CAGGGATGGA GACTCTCATT TGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237267.1
CDS	92..1870
Translation	MPQEQWADRNMDTKDMSEFENEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVPTPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS

Target ORF information:

RefSeq Version	XM_011238965.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, theta (Prkcq), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238965.2

ORF Insert Sequence:

ATGCCGCAAG AACAATGGGC GGACAGAAAT ATGGACACAA AGGACATGAG TGAGTTTGAG 
AATGAAGGAT TCTTTGCACT GCATCAGCGC CGAGGAGCCA TCAAACAGGC CAAAGTCCAC 
CATGTCAAGT GTCACGAGTT CACGGCCACC TTTTTCCCTC AACCCACATT TTGCTCTGTC 
TGCCATGAAT TTGTCTGGGG CCTGAACAAG CAGGGTTACC AGTGCCGACA GTGTAATGCA 
GCGATTCACA AGAAGTGCAT TGATAAAGTG ATAGCCAAGT GCACAGGATC CGCAATCAAT 
AGCCGAGAAA CCATGTTCCA TAAGGAGAGA TTCAAGATCG ACATGCCACA CAGATTCAAA 
GTCTACAACT ACAAGAGTCC AACCTTCTGT GAGCACTGTG GTACCCTGCT CTGGGGGCTG 
GCGAGGCAAG GACTCAAATG TGATGCATGT GGCATGAACG TCCACCACCG ATGCCAGACA 
AAGGTTGCCA ATCTTTGTGG TATAAACCAG AAGCTAATGG CTGAAGCACT AGCGATGATT 
GAAAGCACCC AACAGGCTCG CTCCTTACGA GATTCAGAAC ACATCTTCCG AGAAGGCCCA 
GTTGAAATTG GTCTCCCATG CTCCACCAAA AACGAAACCA GGCCACCATG CGTACCAACA 
CCTGGGAAAA GAGAACCCCA GGGCATTTCC TGGGATTCCC CTTTGGATGG GTCAAATAAA 
TCGGCCGGTC CTCCTGAACC CGAAGTGAGC ATGCGCAGGA CTTCACTGCA GCTGAAACTG 
AAGATCGATG ACTTCATCCT GCACAAGATG TTGGGAAAAG GAAGTTTTGG CAAGGTCTTC 
CTGGCAGAGT TCAAGAGAAC CAATCAGTTT TTCGCAATAA AAGCCTTAAA GAAAGATGTG 
GTGTTGATGG ATGATGACGT CGAGTGTACA ATGGTGGAAA AGAGGGTTCT GTCCTTGGCA 
TGGGAGCATC CATTTCTAAC ACACATGTTC TGCACATTCC AGACCAAGGA AAATCTCTTT 
TTCGTGATGG AGTATCTCAA TGGAGGCGAC TTAATGTACC ACATCCAAAG TTGCCACAAA 
TTTGATCTTT CCAGAGCCAC GTTTTATGCT GCTGAGGTCA TCCTTGGTCT GCAGTTCCTT 
CATTCCAAAG GAATTGTCTA CAGGGACCTG AAGCTTGATA ATATCCTGTT AGACAGAGAT 
GGACATATCA AAATAGCAGA CTTTGGGATG TGCAAAGAGA ACATGCTAGG AGATGCGAAG 
ACAAATACTT TCTGTGGAAC TCCTGACTAC ATTGCTCCGG AGATCTTGCT GGGTCAGAAG 
TACAACCATT CCGTCGACTG GTGGTCCTTC GGGGTGCTCG TTTATGAGAT GCTGATTGGC 
CAGTCCCCCT TCCACGGGCA GGACGAGGAG GAGCTGTTCC ACTCCATCCG CATGGACAAC 
CCCTTCTACC CGAGGTGGCT CGAAAGGGAG GCCAAGGACC TTCTAGTGAA GCTTTTTGTG 
AGAGAACCTG AGAAGAGGCT GGGAGTGAGA GGAGACATCC GCCAGCATCC TTTGTTTCGA 
GAGATCAACT GGGAAGAGCT TGAAAGGAAA GAGATTGACC CACCCTTCAG ACCAAAAGTG 
AAATCACCAT ATGACTGTAG CAATTTCGAC AAGGAATTCC TAAGTGAGAA ACCCCGGCTA 
TCATTCGCCG ACAGAGCACT CATCAACAGC ATGGACCAGA ACATGTTCAG CAACTTTTCC 
TTCATTAACC CAGGGATGGA GACTCTCATT TGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcq ( NM_008859.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032885.1 Mus musculus protein kinase C, theta (Prkcq), mRNA.

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CloneID	OMu19611
Clone ID Related Accession (Same CDS sequence)	NM_008859.2
Accession Version	NM_008859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	protein kinase C theta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL928715.11 and AL929020.10. On Nov 17, 2006 this sequence version replaced NM_008859.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D11091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGTCACCGT TTCTTCGAAT CGGTTTATCC AACTTTGACT GTGGGACCTG CCAAGCTTGT 
CAGGGAGAGG CAGTGAACCC CTACTGCGCT GTGCTTGTCA AAGAGTATGT GGAATCAGAA 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CACCTTGGGA CAGCACCTTT 
GACGCCCACA TTAACAAGGG AAGGGTGATG CAGATCATCG TGAAGGGCAA GAATGTAGAC 
CTCATCTCAG AAACAACCGT GGAACTCTAC TCCCTGGCGG AGAGATGCCG CAAGAACAAT 
GGGCGGACAG AAATATGGTT AGAGCTGAAA CCTCAAGGCC GAATGCTAAT GAATGCAAGA 
TACTTTCTGG AAATGAGTGA CACAAAGGAC ATGAGTGAGT TTGAGAATGA AGGATTCTTT 
GCACTGCATC AGCGCCGAGG AGCCATCAAA CAGGCCAAAG TCCACCATGT CAAGTGTCAC 
GAGTTCACGG CCACCTTTTT CCCTCAACCC ACATTTTGCT CTGTCTGCCA TGAATTTGTC 
TGGGGCCTGA ACAAGCAGGG TTACCAGTGC CGACAGTGTA ATGCAGCGAT TCACAAGAAG 
TGCATTGATA AAGTGATAGC CAAGTGCACA GGATCCGCAA TCAATAGCCG AGAAACCATG 
TTCCATAAGG AGAGATTCAA GATCGACATG CCACACAGAT TCAAAGTCTA CAACTACAAG 
AGTCCAACCT TCTGTGAGCA CTGTGGTACC CTGCTCTGGG GGCTGGCGAG GCAAGGACTC 
AAATGTGATG CATGTGGCAT GAACGTCCAC CACCGATGCC AGACAAAGGT TGCCAATCTT 
TGTGGTATAA ACCAGAAGCT AATGGCTGAA GCACTAGCGA TGATTGAAAG CACCCAACAG 
GCTCGCTCCT TACGAGATTC AGAACACATC TTCCGAGAAG GCCCAGTTGA AATTGGTCTC 
CCATGCTCCA CCAAAAACGA AACCAGGCCA CCATGCGTAC CAACACCTGG GAAAAGAGAA 
CCCCAGGGCA TTTCCTGGGA TTCCCCTTTG GATGGGTCAA ATAAATCGGC CGGTCCTCCT 
GAACCCGAAG TGAGCATGCG CAGGACTTCA CTGCAGCTGA AACTGAAGAT CGATGACTTC 
ATCCTGCACA AGATGTTGGG AAAAGGAAGT TTTGGCAAGG TCTTCCTGGC AGAGTTCAAG 
AGAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTGTT GATGGATGAT 
GACGTCGAGT GTACAATGGT GGAAAAGAGG GTTCTGTCCT TGGCATGGGA GCATCCATTT 
CTAACACACA TGTTCTGCAC ATTCCAGACC AAGGAAAATC TCTTTTTCGT GATGGAGTAT 
CTCAATGGAG GCGACTTAAT GTACCACATC CAAAGTTGCC ACAAATTTGA TCTTTCCAGA 
GCCACGTTTT ATGCTGCTGA GGTCATCCTT GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT TGATAATATC CTGTTAGACA GAGATGGACA TATCAAAATA 
GCAGACTTTG GGATGTGCAA AGAGAACATG CTAGGAGATG CGAAGACAAA TACTTTCTGT 
GGAACTCCTG ACTACATTGC TCCGGAGATC TTGCTGGGTC AGAAGTACAA CCATTCCGTC 
GACTGGTGGT CCTTCGGGGT GCTCGTTTAT GAGATGCTGA TTGGCCAGTC CCCCTTCCAC 
GGGCAGGACG AGGAGGAGCT GTTCCACTCC ATCCGCATGG ACAACCCCTT CTACCCGAGG 
TGGCTCGAAA GGGAGGCCAA GGACCTTCTA GTGAAGCTTT TTGTGAGAGA ACCTGAGAAG 
AGGCTGGGAG TGAGAGGAGA CATCCGCCAG CATCCTTTGT TTCGAGAGAT CAACTGGGAA 
GAGCTTGAAA GGAAAGAGAT TGACCCACCC TTCAGACCAA AAGTGAAATC ACCATATGAC 
TGTAGCAATT TCGACAAGGA ATTCCTAAGT GAGAAACCCC GGCTATCATT CGCCGACAGA 
GCACTCATCA ACAGCATGGA CCAGAACATG TTCAGCAACT TTTCCTTCAT TAACCCAGGG 
ATGGAGACTC TCATTTGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032885.1
CDS	89..2212
Translation	MSPFLRIGLSNFDCGTCQACQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGRTEIWLELKPQGRMLMNARYFLEMSDTKDMSEFENEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVPTPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS

Target ORF information:

RefSeq Version	NM_008859.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, theta (Prkcq), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008859.2

ORF Insert Sequence:

ATGTCACCGT TTCTTCGAAT CGGTTTATCC AACTTTGACT GTGGGACCTG CCAAGCTTGT 
CAGGGAGAGG CAGTGAACCC CTACTGCGCT GTGCTTGTCA AAGAGTATGT GGAATCAGAA 
AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CACCTTGGGA CAGCACCTTT 
GACGCCCACA TTAACAAGGG AAGGGTGATG CAGATCATCG TGAAGGGCAA GAATGTAGAC 
CTCATCTCAG AAACAACCGT GGAACTCTAC TCCCTGGCGG AGAGATGCCG CAAGAACAAT 
GGGCGGACAG AAATATGGTT AGAGCTGAAA CCTCAAGGCC GAATGCTAAT GAATGCAAGA 
TACTTTCTGG AAATGAGTGA CACAAAGGAC ATGAGTGAGT TTGAGAATGA AGGATTCTTT 
GCACTGCATC AGCGCCGAGG AGCCATCAAA CAGGCCAAAG TCCACCATGT CAAGTGTCAC 
GAGTTCACGG CCACCTTTTT CCCTCAACCC ACATTTTGCT CTGTCTGCCA TGAATTTGTC 
TGGGGCCTGA ACAAGCAGGG TTACCAGTGC CGACAGTGTA ATGCAGCGAT TCACAAGAAG 
TGCATTGATA AAGTGATAGC CAAGTGCACA GGATCCGCAA TCAATAGCCG AGAAACCATG 
TTCCATAAGG AGAGATTCAA GATCGACATG CCACACAGAT TCAAAGTCTA CAACTACAAG 
AGTCCAACCT TCTGTGAGCA CTGTGGTACC CTGCTCTGGG GGCTGGCGAG GCAAGGACTC 
AAATGTGATG CATGTGGCAT GAACGTCCAC CACCGATGCC AGACAAAGGT TGCCAATCTT 
TGTGGTATAA ACCAGAAGCT AATGGCTGAA GCACTAGCGA TGATTGAAAG CACCCAACAG 
GCTCGCTCCT TACGAGATTC AGAACACATC TTCCGAGAAG GCCCAGTTGA AATTGGTCTC 
CCATGCTCCA CCAAAAACGA AACCAGGCCA CCATGCGTAC CAACACCTGG GAAAAGAGAA 
CCCCAGGGCA TTTCCTGGGA TTCCCCTTTG GATGGGTCAA ATAAATCGGC CGGTCCTCCT 
GAACCCGAAG TGAGCATGCG CAGGACTTCA CTGCAGCTGA AACTGAAGAT CGATGACTTC 
ATCCTGCACA AGATGTTGGG AAAAGGAAGT TTTGGCAAGG TCTTCCTGGC AGAGTTCAAG 
AGAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTGTT GATGGATGAT 
GACGTCGAGT GTACAATGGT GGAAAAGAGG GTTCTGTCCT TGGCATGGGA GCATCCATTT 
CTAACACACA TGTTCTGCAC ATTCCAGACC AAGGAAAATC TCTTTTTCGT GATGGAGTAT 
CTCAATGGAG GCGACTTAAT GTACCACATC CAAAGTTGCC ACAAATTTGA TCTTTCCAGA 
GCCACGTTTT ATGCTGCTGA GGTCATCCTT GGTCTGCAGT TCCTTCATTC CAAAGGAATT 
GTCTACAGGG ACCTGAAGCT TGATAATATC CTGTTAGACA GAGATGGACA TATCAAAATA 
GCAGACTTTG GGATGTGCAA AGAGAACATG CTAGGAGATG CGAAGACAAA TACTTTCTGT 
GGAACTCCTG ACTACATTGC TCCGGAGATC TTGCTGGGTC AGAAGTACAA CCATTCCGTC 
GACTGGTGGT CCTTCGGGGT GCTCGTTTAT GAGATGCTGA TTGGCCAGTC CCCCTTCCAC 
GGGCAGGACG AGGAGGAGCT GTTCCACTCC ATCCGCATGG ACAACCCCTT CTACCCGAGG 
TGGCTCGAAA GGGAGGCCAA GGACCTTCTA GTGAAGCTTT TTGTGAGAGA ACCTGAGAAG 
AGGCTGGGAG TGAGAGGAGA CATCCGCCAG CATCCTTTGT TTCGAGAGAT CAACTGGGAA 
GAGCTTGAAA GGAAAGAGAT TGACCCACCC TTCAGACCAA AAGTGAAATC ACCATATGAC 
TGTAGCAATT TCGACAAGGA ATTCCTAAGT GAGAAACCCC GGCTATCATT CGCCGACAGA 
GCACTCATCA ACAGCATGGA CCAGAACATG TTCAGCAACT TTTCCTTCAT TAACCCAGGG 
ATGGAGACTC TCATTTGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcq ( XM_006497398.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497461.1 Mus musculus protein kinase C, theta (Prkcq), transcript variant X1, mRNA.

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CloneID	OMu29094
Clone ID Related Accession (Same CDS sequence)	XM_006497398.3 , XM_006497398.4
Accession Version	XM_006497398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kinase C theta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497398.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCC AGAAAAAGCC AACCATGTAC CCACCTTGGG ACAGCACCTT TGACGCCCAC 
ATTAACAAGG GAAGGGTGAT GCAGATCATC GTGAAGGGCA AGAATGTAGA CCTCATCTCA 
GAAACAACCG TGGAACTCTA CTCCCTGGCG GAGAGATGCC GCAAGAACAA TGGGCGGACA 
GAAATATGGT TAGAGCTGAA ACCTCAAGGC CGAATGCTAA TGAATGCAAG ATACTTTCTG 
GAAATGAGTG ACACAAAGGA CATGAGTGAG TTTGAGAATG AAGGATTCTT TGCACTGCAT 
CAGCGCCGAG GAGCCATCAA ACAGGCCAAA GTCCACCATG TCAAGTGTCA CGAGTTCACG 
GCCACCTTTT TCCCTCAACC CACATTTTGC TCTGTCTGCC ATGAATTTGT CTGGGGCCTG 
AACAAGCAGG GTTACCAGTG CCGACAGTGT AATGCAGCGA TTCACAAGAA GTGCATTGAT 
AAAGTGATAG CCAAGTGCAC AGGATCCGCA ATCAATAGCC GAGAAACCAT GTTCCATAAG 
GAGAGATTCA AGATCGACAT GCCACACAGA TTCAAAGTCT ACAACTACAA GAGTCCAACC 
TTCTGTGAGC ACTGTGGTAC CCTGCTCTGG GGGCTGGCGA GGCAAGGACT CAAATGTGAT 
GCATGTGGCA TGAACGTCCA CCACCGATGC CAGACAAAGG TTGCCAATCT TTGTGGTATA 
AACCAGAAGC TAATGGCTGA AGCACTAGCG ATGATTGAAA GCACCCAACA GGCTCGCTCC 
TTACGAGATT CAGAACACAT CTTCCGAGAA GGCCCAGTTG AAATTGGTCT CCCATGCTCC 
ACCAAAAACG AAACCAGGCC ACCATGCGTA CCAACACCTG GGAAAAGAGA ACCCCAGGGC 
ATTTCCTGGG ATTCCCCTTT GGATGGGTCA AATAAATCGG CCGGTCCTCC TGAACCCGAA 
GTGAGCATGC GCAGGACTTC ACTGCAGCTG AAACTGAAGA TCGATGACTT CATCCTGCAC 
AAGATGTTGG GAAAAGGAAG TTTTGGCAAG GTCTTCCTGG CAGAGTTCAA GAGAACCAAT 
CAGTTTTTCG CAATAAAAGC CTTAAAGAAA GATGTGGTGT TGATGGATGA TGACGTCGAG 
TGTACAATGG TGGAAAAGAG GGTTCTGTCC TTGGCATGGG AGCATCCATT TCTAACACAC 
ATGTTCTGCA CATTCCAGAC CAAGGAAAAT CTCTTTTTCG TGATGGAGTA TCTCAATGGA 
GGCGACTTAA TGTACCACAT CCAAAGTTGC CACAAATTTG ATCTTTCCAG AGCCACGTTT 
TATGCTGCTG AGGTCATCCT TGGTCTGCAG TTCCTTCATT CCAAAGGAAT TGTCTACAGG 
GACCTGAAGC TTGATAATAT CCTGTTAGAC AGAGATGGAC ATATCAAAAT AGCAGACTTT 
GGGATGTGCA AAGAGAACAT GCTAGGAGAT GCGAAGACAA ATACTTTCTG TGGAACTCCT 
GACTACATTG CTCCGGAGAT CTTGCTGGGT CAGAAGTACA ACCATTCCGT CGACTGGTGG 
TCCTTCGGGG TGCTCGTTTA TGAGATGCTG ATTGGCCAGT CCCCCTTCCA CGGGCAGGAC 
GAGGAGGAGC TGTTCCACTC CATCCGCATG GACAACCCCT TCTACCCGAG GTGGCTCGAA 
AGGGAGGCCA AGGACCTTCT AGTGAAGCTT TTTGTGAGAG AACCTGAGAA GAGGCTGGGA 
GTGAGAGGAG ACATCCGCCA GCATCCTTTG TTTCGAGAGA TCAACTGGGA AGAGCTTGAA 
AGGAAAGAGA TTGACCCACC CTTCAGACCA AAAGTGAAAT CACCATATGA CTGTAGCAAT 
TTCGACAAGG AATTCCTAAG TGAGAAACCC CGGCTATCAT TCGCCGACAG AGCACTCATC 
AACAGCATGG ACCAGAACAT GTTCAGCAAC TTTTCCTTCA TTAACCCAGG GATGGAGACT 
CTCATTTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497461.1
CDS	568..2562
Translation	MYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGRTEIWLELKPQGRMLMNARYFLEMSDTKDMSEFENEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVPTPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS

Target ORF information:

RefSeq Version	XM_006497398.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, theta (Prkcq), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497398.4

ORF Insert Sequence:

ATGTACATCC AGAAAAAGCC AACCATGTAC CCACCTTGGG ACAGCACCTT TGACGCCCAC 
ATTAACAAGG GAAGGGTGAT GCAGATCATC GTGAAGGGCA AGAATGTAGA CCTCATCTCA 
GAAACAACCG TGGAACTCTA CTCCCTGGCG GAGAGATGCC GCAAGAACAA TGGGCGGACA 
GAAATATGGT TAGAGCTGAA ACCTCAAGGC CGAATGCTAA TGAATGCAAG ATACTTTCTG 
GAAATGAGTG ACACAAAGGA CATGAGTGAG TTTGAGAATG AAGGATTCTT TGCACTGCAT 
CAGCGCCGAG GAGCCATCAA ACAGGCCAAA GTCCACCATG TCAAGTGTCA CGAGTTCACG 
GCCACCTTTT TCCCTCAACC CACATTTTGC TCTGTCTGCC ATGAATTTGT CTGGGGCCTG 
AACAAGCAGG GTTACCAGTG CCGACAGTGT AATGCAGCGA TTCACAAGAA GTGCATTGAT 
AAAGTGATAG CCAAGTGCAC AGGATCCGCA ATCAATAGCC GAGAAACCAT GTTCCATAAG 
GAGAGATTCA AGATCGACAT GCCACACAGA TTCAAAGTCT ACAACTACAA GAGTCCAACC 
TTCTGTGAGC ACTGTGGTAC CCTGCTCTGG GGGCTGGCGA GGCAAGGACT CAAATGTGAT 
GCATGTGGCA TGAACGTCCA CCACCGATGC CAGACAAAGG TTGCCAATCT TTGTGGTATA 
AACCAGAAGC TAATGGCTGA AGCACTAGCG ATGATTGAAA GCACCCAACA GGCTCGCTCC 
TTACGAGATT CAGAACACAT CTTCCGAGAA GGCCCAGTTG AAATTGGTCT CCCATGCTCC 
ACCAAAAACG AAACCAGGCC ACCATGCGTA CCAACACCTG GGAAAAGAGA ACCCCAGGGC 
ATTTCCTGGG ATTCCCCTTT GGATGGGTCA AATAAATCGG CCGGTCCTCC TGAACCCGAA 
GTGAGCATGC GCAGGACTTC ACTGCAGCTG AAACTGAAGA TCGATGACTT CATCCTGCAC 
AAGATGTTGG GAAAAGGAAG TTTTGGCAAG GTCTTCCTGG CAGAGTTCAA GAGAACCAAT 
CAGTTTTTCG CAATAAAAGC CTTAAAGAAA GATGTGGTGT TGATGGATGA TGACGTCGAG 
TGTACAATGG TGGAAAAGAG GGTTCTGTCC TTGGCATGGG AGCATCCATT TCTAACACAC 
ATGTTCTGCA CATTCCAGAC CAAGGAAAAT CTCTTTTTCG TGATGGAGTA TCTCAATGGA 
GGCGACTTAA TGTACCACAT CCAAAGTTGC CACAAATTTG ATCTTTCCAG AGCCACGTTT 
TATGCTGCTG AGGTCATCCT TGGTCTGCAG TTCCTTCATT CCAAAGGAAT TGTCTACAGG 
GACCTGAAGC TTGATAATAT CCTGTTAGAC AGAGATGGAC ATATCAAAAT AGCAGACTTT 
GGGATGTGCA AAGAGAACAT GCTAGGAGAT GCGAAGACAA ATACTTTCTG TGGAACTCCT 
GACTACATTG CTCCGGAGAT CTTGCTGGGT CAGAAGTACA ACCATTCCGT CGACTGGTGG 
TCCTTCGGGG TGCTCGTTTA TGAGATGCTG ATTGGCCAGT CCCCCTTCCA CGGGCAGGAC 
GAGGAGGAGC TGTTCCACTC CATCCGCATG GACAACCCCT TCTACCCGAG GTGGCTCGAA 
AGGGAGGCCA AGGACCTTCT AGTGAAGCTT TTTGTGAGAG AACCTGAGAA GAGGCTGGGA 
GTGAGAGGAG ACATCCGCCA GCATCCTTTG TTTCGAGAGA TCAACTGGGA AGAGCTTGAA 
AGGAAAGAGA TTGACCCACC CTTCAGACCA AAAGTGAAAT CACCATATGA CTGTAGCAAT 
TTCGACAAGG AATTCCTAAG TGAGAAACCC CGGCTATCAT TCGCCGACAG AGCACTCATC 
AACAGCATGG ACCAGAACAT GTTCAGCAAC TTTTCCTTCA TTAACCCAGG GATGGAGACT 
CTCATTTGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcq ( XM_030248462.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104322.1 Mus musculus protein kinase C, theta (Prkcq), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68442
Clone ID Related Accession (Same CDS sequence)	XM_030248462.1 , XM_011238965.2 , XM_030248463.1
Accession Version	XM_030248462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kinase C theta type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAAG AACAATGGGC GGACAGAAAT ATGGACACAA AGGACATGAG TGAGTTTGAG 
AATGAAGGAT TCTTTGCACT GCATCAGCGC CGAGGAGCCA TCAAACAGGC CAAAGTCCAC 
CATGTCAAGT GTCACGAGTT CACGGCCACC TTTTTCCCTC AACCCACATT TTGCTCTGTC 
TGCCATGAAT TTGTCTGGGG CCTGAACAAG CAGGGTTACC AGTGCCGACA GTGTAATGCA 
GCGATTCACA AGAAGTGCAT TGATAAAGTG ATAGCCAAGT GCACAGGATC CGCAATCAAT 
AGCCGAGAAA CCATGTTCCA TAAGGAGAGA TTCAAGATCG ACATGCCACA CAGATTCAAA 
GTCTACAACT ACAAGAGTCC AACCTTCTGT GAGCACTGTG GTACCCTGCT CTGGGGGCTG 
GCGAGGCAAG GACTCAAATG TGATGCATGT GGCATGAACG TCCACCACCG ATGCCAGACA 
AAGGTTGCCA ATCTTTGTGG TATAAACCAG AAGCTAATGG CTGAAGCACT AGCGATGATT 
GAAAGCACCC AACAGGCTCG CTCCTTACGA GATTCAGAAC ACATCTTCCG AGAAGGCCCA 
GTTGAAATTG GTCTCCCATG CTCCACCAAA AACGAAACCA GGCCACCATG CGTACCAACA 
CCTGGGAAAA GAGAACCCCA GGGCATTTCC TGGGATTCCC CTTTGGATGG GTCAAATAAA 
TCGGCCGGTC CTCCTGAACC CGAAGTGAGC ATGCGCAGGA CTTCACTGCA GCTGAAACTG 
AAGATCGATG ACTTCATCCT GCACAAGATG TTGGGAAAAG GAAGTTTTGG CAAGGTCTTC 
CTGGCAGAGT TCAAGAGAAC CAATCAGTTT TTCGCAATAA AAGCCTTAAA GAAAGATGTG 
GTGTTGATGG ATGATGACGT CGAGTGTACA ATGGTGGAAA AGAGGGTTCT GTCCTTGGCA 
TGGGAGCATC CATTTCTAAC ACACATGTTC TGCACATTCC AGACCAAGGA AAATCTCTTT 
TTCGTGATGG AGTATCTCAA TGGAGGCGAC TTAATGTACC ACATCCAAAG TTGCCACAAA 
TTTGATCTTT CCAGAGCCAC GTTTTATGCT GCTGAGGTCA TCCTTGGTCT GCAGTTCCTT 
CATTCCAAAG GAATTGTCTA CAGGGACCTG AAGCTTGATA ATATCCTGTT AGACAGAGAT 
GGACATATCA AAATAGCAGA CTTTGGGATG TGCAAAGAGA ACATGCTAGG AGATGCGAAG 
ACAAATACTT TCTGTGGAAC TCCTGACTAC ATTGCTCCGG AGATCTTGCT GGGTCAGAAG 
TACAACCATT CCGTCGACTG GTGGTCCTTC GGGGTGCTCG TTTATGAGAT GCTGATTGGC 
CAGTCCCCCT TCCACGGGCA GGACGAGGAG GAGCTGTTCC ACTCCATCCG CATGGACAAC 
CCCTTCTACC CGAGGTGGCT CGAAAGGGAG GCCAAGGACC TTCTAGTGAA GCTTTTTGTG 
AGAGAACCTG AGAAGAGGCT GGGAGTGAGA GGAGACATCC GCCAGCATCC TTTGTTTCGA 
GAGATCAACT GGGAAGAGCT TGAAAGGAAA GAGATTGACC CACCCTTCAG ACCAAAAGTG 
AAATCACCAT ATGACTGTAG CAATTTCGAC AAGGAATTCC TAAGTGAGAA ACCCCGGCTA 
TCATTCGCCG ACAGAGCACT CATCAACAGC ATGGACCAGA ACATGTTCAG CAACTTTTCC 
TTCATTAACC CAGGGATGGA GACTCTCATT TGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104322.1
CDS	850..2628
Translation	MPQEQWADRNMDTKDMSEFENEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVPTPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS

Target ORF information:

RefSeq Version	XM_030248462.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, theta (Prkcq), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248462.1

ORF Insert Sequence:

ATGCCGCAAG AACAATGGGC GGACAGAAAT ATGGACACAA AGGACATGAG TGAGTTTGAG 
AATGAAGGAT TCTTTGCACT GCATCAGCGC CGAGGAGCCA TCAAACAGGC CAAAGTCCAC 
CATGTCAAGT GTCACGAGTT CACGGCCACC TTTTTCCCTC AACCCACATT TTGCTCTGTC 
TGCCATGAAT TTGTCTGGGG CCTGAACAAG CAGGGTTACC AGTGCCGACA GTGTAATGCA 
GCGATTCACA AGAAGTGCAT TGATAAAGTG ATAGCCAAGT GCACAGGATC CGCAATCAAT 
AGCCGAGAAA CCATGTTCCA TAAGGAGAGA TTCAAGATCG ACATGCCACA CAGATTCAAA 
GTCTACAACT ACAAGAGTCC AACCTTCTGT GAGCACTGTG GTACCCTGCT CTGGGGGCTG 
GCGAGGCAAG GACTCAAATG TGATGCATGT GGCATGAACG TCCACCACCG ATGCCAGACA 
AAGGTTGCCA ATCTTTGTGG TATAAACCAG AAGCTAATGG CTGAAGCACT AGCGATGATT 
GAAAGCACCC AACAGGCTCG CTCCTTACGA GATTCAGAAC ACATCTTCCG AGAAGGCCCA 
GTTGAAATTG GTCTCCCATG CTCCACCAAA AACGAAACCA GGCCACCATG CGTACCAACA 
CCTGGGAAAA GAGAACCCCA GGGCATTTCC TGGGATTCCC CTTTGGATGG GTCAAATAAA 
TCGGCCGGTC CTCCTGAACC CGAAGTGAGC ATGCGCAGGA CTTCACTGCA GCTGAAACTG 
AAGATCGATG ACTTCATCCT GCACAAGATG TTGGGAAAAG GAAGTTTTGG CAAGGTCTTC 
CTGGCAGAGT TCAAGAGAAC CAATCAGTTT TTCGCAATAA AAGCCTTAAA GAAAGATGTG 
GTGTTGATGG ATGATGACGT CGAGTGTACA ATGGTGGAAA AGAGGGTTCT GTCCTTGGCA 
TGGGAGCATC CATTTCTAAC ACACATGTTC TGCACATTCC AGACCAAGGA AAATCTCTTT 
TTCGTGATGG AGTATCTCAA TGGAGGCGAC TTAATGTACC ACATCCAAAG TTGCCACAAA 
TTTGATCTTT CCAGAGCCAC GTTTTATGCT GCTGAGGTCA TCCTTGGTCT GCAGTTCCTT 
CATTCCAAAG GAATTGTCTA CAGGGACCTG AAGCTTGATA ATATCCTGTT AGACAGAGAT 
GGACATATCA AAATAGCAGA CTTTGGGATG TGCAAAGAGA ACATGCTAGG AGATGCGAAG 
ACAAATACTT TCTGTGGAAC TCCTGACTAC ATTGCTCCGG AGATCTTGCT GGGTCAGAAG 
TACAACCATT CCGTCGACTG GTGGTCCTTC GGGGTGCTCG TTTATGAGAT GCTGATTGGC 
CAGTCCCCCT TCCACGGGCA GGACGAGGAG GAGCTGTTCC ACTCCATCCG CATGGACAAC 
CCCTTCTACC CGAGGTGGCT CGAAAGGGAG GCCAAGGACC TTCTAGTGAA GCTTTTTGTG 
AGAGAACCTG AGAAGAGGCT GGGAGTGAGA GGAGACATCC GCCAGCATCC TTTGTTTCGA 
GAGATCAACT GGGAAGAGCT TGAAAGGAAA GAGATTGACC CACCCTTCAG ACCAAAAGTG 
AAATCACCAT ATGACTGTAG CAATTTCGAC AAGGAATTCC TAAGTGAGAA ACCCCGGCTA 
TCATTCGCCG ACAGAGCACT CATCAACAGC ATGGACCAGA ACATGTTCAG CAACTTTTCC 
TTCATTAACC CAGGGATGGA GACTCTCATT TGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcq ( XM_030248463.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104323.1 Mus musculus protein kinase C, theta (Prkcq), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu68442
Clone ID Related Accession (Same CDS sequence)	XM_030248462.1 , XM_011238965.2 , XM_030248463.1
Accession Version	XM_030248463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kinase C theta type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCAAG AACAATGGGC GGACAGAAAT ATGGACACAA AGGACATGAG TGAGTTTGAG 
AATGAAGGAT TCTTTGCACT GCATCAGCGC CGAGGAGCCA TCAAACAGGC CAAAGTCCAC 
CATGTCAAGT GTCACGAGTT CACGGCCACC TTTTTCCCTC AACCCACATT TTGCTCTGTC 
TGCCATGAAT TTGTCTGGGG CCTGAACAAG CAGGGTTACC AGTGCCGACA GTGTAATGCA 
GCGATTCACA AGAAGTGCAT TGATAAAGTG ATAGCCAAGT GCACAGGATC CGCAATCAAT 
AGCCGAGAAA CCATGTTCCA TAAGGAGAGA TTCAAGATCG ACATGCCACA CAGATTCAAA 
GTCTACAACT ACAAGAGTCC AACCTTCTGT GAGCACTGTG GTACCCTGCT CTGGGGGCTG 
GCGAGGCAAG GACTCAAATG TGATGCATGT GGCATGAACG TCCACCACCG ATGCCAGACA 
AAGGTTGCCA ATCTTTGTGG TATAAACCAG AAGCTAATGG CTGAAGCACT AGCGATGATT 
GAAAGCACCC AACAGGCTCG CTCCTTACGA GATTCAGAAC ACATCTTCCG AGAAGGCCCA 
GTTGAAATTG GTCTCCCATG CTCCACCAAA AACGAAACCA GGCCACCATG CGTACCAACA 
CCTGGGAAAA GAGAACCCCA GGGCATTTCC TGGGATTCCC CTTTGGATGG GTCAAATAAA 
TCGGCCGGTC CTCCTGAACC CGAAGTGAGC ATGCGCAGGA CTTCACTGCA GCTGAAACTG 
AAGATCGATG ACTTCATCCT GCACAAGATG TTGGGAAAAG GAAGTTTTGG CAAGGTCTTC 
CTGGCAGAGT TCAAGAGAAC CAATCAGTTT TTCGCAATAA AAGCCTTAAA GAAAGATGTG 
GTGTTGATGG ATGATGACGT CGAGTGTACA ATGGTGGAAA AGAGGGTTCT GTCCTTGGCA 
TGGGAGCATC CATTTCTAAC ACACATGTTC TGCACATTCC AGACCAAGGA AAATCTCTTT 
TTCGTGATGG AGTATCTCAA TGGAGGCGAC TTAATGTACC ACATCCAAAG TTGCCACAAA 
TTTGATCTTT CCAGAGCCAC GTTTTATGCT GCTGAGGTCA TCCTTGGTCT GCAGTTCCTT 
CATTCCAAAG GAATTGTCTA CAGGGACCTG AAGCTTGATA ATATCCTGTT AGACAGAGAT 
GGACATATCA AAATAGCAGA CTTTGGGATG TGCAAAGAGA ACATGCTAGG AGATGCGAAG 
ACAAATACTT TCTGTGGAAC TCCTGACTAC ATTGCTCCGG AGATCTTGCT GGGTCAGAAG 
TACAACCATT CCGTCGACTG GTGGTCCTTC GGGGTGCTCG TTTATGAGAT GCTGATTGGC 
CAGTCCCCCT TCCACGGGCA GGACGAGGAG GAGCTGTTCC ACTCCATCCG CATGGACAAC 
CCCTTCTACC CGAGGTGGCT CGAAAGGGAG GCCAAGGACC TTCTAGTGAA GCTTTTTGTG 
AGAGAACCTG AGAAGAGGCT GGGAGTGAGA GGAGACATCC GCCAGCATCC TTTGTTTCGA 
GAGATCAACT GGGAAGAGCT TGAAAGGAAA GAGATTGACC CACCCTTCAG ACCAAAAGTG 
AAATCACCAT ATGACTGTAG CAATTTCGAC AAGGAATTCC TAAGTGAGAA ACCCCGGCTA 
TCATTCGCCG ACAGAGCACT CATCAACAGC ATGGACCAGA ACATGTTCAG CAACTTTTCC 
TTCATTAACC CAGGGATGGA GACTCTCATT TGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104323.1
CDS	723..2501
Translation	MPQEQWADRNMDTKDMSEFENEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARSLRDSEHIFREGPVEIGLPCSTKNETRPPCVPTPGKREPQGISWDSPLDGSNKSAGPPEPEVSMRRTSLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS

Target ORF information:

RefSeq Version	XM_030248463.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, theta (Prkcq), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248463.1

ORF Insert Sequence:

ATGCCGCAAG AACAATGGGC GGACAGAAAT ATGGACACAA AGGACATGAG TGAGTTTGAG 
AATGAAGGAT TCTTTGCACT GCATCAGCGC CGAGGAGCCA TCAAACAGGC CAAAGTCCAC 
CATGTCAAGT GTCACGAGTT CACGGCCACC TTTTTCCCTC AACCCACATT TTGCTCTGTC 
TGCCATGAAT TTGTCTGGGG CCTGAACAAG CAGGGTTACC AGTGCCGACA GTGTAATGCA 
GCGATTCACA AGAAGTGCAT TGATAAAGTG ATAGCCAAGT GCACAGGATC CGCAATCAAT 
AGCCGAGAAA CCATGTTCCA TAAGGAGAGA TTCAAGATCG ACATGCCACA CAGATTCAAA 
GTCTACAACT ACAAGAGTCC AACCTTCTGT GAGCACTGTG GTACCCTGCT CTGGGGGCTG 
GCGAGGCAAG GACTCAAATG TGATGCATGT GGCATGAACG TCCACCACCG ATGCCAGACA 
AAGGTTGCCA ATCTTTGTGG TATAAACCAG AAGCTAATGG CTGAAGCACT AGCGATGATT 
GAAAGCACCC AACAGGCTCG CTCCTTACGA GATTCAGAAC ACATCTTCCG AGAAGGCCCA 
GTTGAAATTG GTCTCCCATG CTCCACCAAA AACGAAACCA GGCCACCATG CGTACCAACA 
CCTGGGAAAA GAGAACCCCA GGGCATTTCC TGGGATTCCC CTTTGGATGG GTCAAATAAA 
TCGGCCGGTC CTCCTGAACC CGAAGTGAGC ATGCGCAGGA CTTCACTGCA GCTGAAACTG 
AAGATCGATG ACTTCATCCT GCACAAGATG TTGGGAAAAG GAAGTTTTGG CAAGGTCTTC 
CTGGCAGAGT TCAAGAGAAC CAATCAGTTT TTCGCAATAA AAGCCTTAAA GAAAGATGTG 
GTGTTGATGG ATGATGACGT CGAGTGTACA ATGGTGGAAA AGAGGGTTCT GTCCTTGGCA 
TGGGAGCATC CATTTCTAAC ACACATGTTC TGCACATTCC AGACCAAGGA AAATCTCTTT 
TTCGTGATGG AGTATCTCAA TGGAGGCGAC TTAATGTACC ACATCCAAAG TTGCCACAAA 
TTTGATCTTT CCAGAGCCAC GTTTTATGCT GCTGAGGTCA TCCTTGGTCT GCAGTTCCTT 
CATTCCAAAG GAATTGTCTA CAGGGACCTG AAGCTTGATA ATATCCTGTT AGACAGAGAT 
GGACATATCA AAATAGCAGA CTTTGGGATG TGCAAAGAGA ACATGCTAGG AGATGCGAAG 
ACAAATACTT TCTGTGGAAC TCCTGACTAC ATTGCTCCGG AGATCTTGCT GGGTCAGAAG 
TACAACCATT CCGTCGACTG GTGGTCCTTC GGGGTGCTCG TTTATGAGAT GCTGATTGGC 
CAGTCCCCCT TCCACGGGCA GGACGAGGAG GAGCTGTTCC ACTCCATCCG CATGGACAAC 
CCCTTCTACC CGAGGTGGCT CGAAAGGGAG GCCAAGGACC TTCTAGTGAA GCTTTTTGTG 
AGAGAACCTG AGAAGAGGCT GGGAGTGAGA GGAGACATCC GCCAGCATCC TTTGTTTCGA 
GAGATCAACT GGGAAGAGCT TGAAAGGAAA GAGATTGACC CACCCTTCAG ACCAAAAGTG 
AAATCACCAT ATGACTGTAG CAATTTCGAC AAGGAATTCC TAAGTGAGAA ACCCCGGCTA 
TCATTCGCCG ACAGAGCACT CATCAACAGC ATGGACCAGA ACATGTTCAG CAACTTTTCC 
TTCATTAACC CAGGGATGGA GACTCTCATT TGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SRC1 promotes Th17 differentiation by overriding Foxp3 suppression to stimulate ROR?t activity in a PKC-?-dependent manner.
Proceedings of the National Academy of Sciences of the United States of America115(3)E458-E467(2018 Jan)
Sen S,Wang F,Zhang J,He Z,Ma J,Gwack Y,Xu J,Sun Z

Targeting early PKC?-dependent T-cell infiltration of dystrophic muscle reduces disease severity in a mouse model of muscular dystrophy.
The Journal of pathology(2017 Dec)
Lozanoska-Ochser B,Benedetti A,Rizzo G,Marrocco V,Di Maggio R,Fiore P,Bouche M

Mice Lacking PKC? in Skeletal Muscle Have Reduced Intramyocellular Lipid Accumulation and Increased Insulin Responsiveness in Skeletal Muscle.
American journal of physiology. Regulatory, integrative and comparative physiologyajpregu005212016(2017 Nov)
Peck B,Huot J,Renzi T,Arthur ST,Turner MJ,Marino JS

PKC-? is dispensable for OX40L-induced TCR-independent Treg proliferation but contributes by enabling IL-2 production from effector T-cells.
Scientific reports7(1)6594(2017 Jul)
Alharshawi K,Marinelarena A,Kumar P,El-Sayed O,Bhattacharya P,Sun Z,Epstein AL,Maker AV,Prabhakar BS

Human in vitro-induced regulatory T cells display Dlgh1dependent and PKC-? restrained suppressive activity.
Scientific reports7(1)4258(2017 Jun)
Zanin-Zhorov A,Kumari S,Hippen KL,Merkel SC,MacMillan ML,Blazar BR,Dustin ML

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Prkcq cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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