Prkce cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkce
Entrez Gene ID	18754
Full Name	protein kinase C, epsilon
Synonyms	5830406C15Rik,PKC[e],PKCepsilon,Pkce,R75156
General protein information	Preferred Names protein kinase C, epsilon Names protein kinase C epsilon type nPKC-epsilon
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E4

mRNA and Protein(s)

mRNA	Protein	Name
XM_017317328.1	XP_017172817.1	protein kinase C epsilon type isoform X1
NM_011104.3	NP_035234.1	protein kinase C epsilon type
XM_006523833.2	XP_006523896.1	protein kinase C epsilon type isoform X2
XM_030249576.1	XP_030105436.1	protein kinase C epsilon type isoform X3
XM_017317328.2	XP_017172817.1	protein kinase C epsilon type isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRKCE	XP_851861.1
Drosophila melanogaster (fruit fly)	Pkc98E	NP_524545.2
Schizosaccharomyces pombe (fission yeast)	pck2	NP_595950.1
Homo sapiens (human)	PRKCE	NP_005391.1
Danio rerio (zebrafish)	prkcea	XP_005156351.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002748	XP_312175.5
Rattus norvegicus (Norway rat)	Prkce	NP_058867.1
Danio rerio (zebrafish)	prkceb	XP_002664164.2
Saccharomyces cerevisiae (baker's yeast)	PKC1	NP_009445.2
Eremothecium gossypii	AGOS_ACR191C	NP_983593.1
Xenopus tropicalis (tropical clawed frog)	prkce	NP_001107724.1
Bos taurus (cattle)	PRKCE	NP_001104590.1
Gallus gallus (chicken)	PRKCE	XP_419464.3
Caenorhabditis elegans (roundworm)	pkc-1	NP_001256395.1
Kluyveromyces lactis	KLLA0E06447g	XP_454238.1
Magnaporthe oryzae (rice blast fungus)	MGG_08689	XP_003719292.1
Neurospora crassa	NCU06544	XP_962251.1
Pan troglodytes (chimpanzee)	PRKCE	XP_003309040.1
	PRKCE	XP_001112763.1
Mus musculus (house mouse)	Prkce	NP_035234.1

Gene Ontology
Bibliography

Related articles in PubMed

O-GlcNAc on PKC? Inhibits the FGF4-PKC?-MEK-ERK1/2 Pathway via Inhibition of PKC? Phosphorylation in Mouse Embryonic Stem Cells.
Miura T, Kume M, Kawamura T, Yamamoto K, Hamakubo T, Nishihara S
Stem cell reports10(1)272-286(2018 Jan)

PKC-epsilon deficiency alters progenitor cell populations in favor of megakaryopoiesis.
Kostyak JC, Liverani E, Kunapuli SP
PloS one12(8)e0182867(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that PKCe positively regulates beta-catenin expression and stabilization in a glycogen synthase kinase 3beta-independent manner; beta-catenin exhibits a perinuclear localization pattern in podocytes; however, beta-catenin is predominantly localized to nucleus in podocytes from PKCe knockout mice. (PKCe = protein kinase C epsilon)
Title: Protein kinase C ? stabilizes ?-catenin and regulates its subcellular localization in podocytes.

study establishes that the pseudosubstrate of PKC-epsilon binds phosphatidylinositol 4-phosphate (PI4P), tethering it to the trans-Golgi network (TGN). This TGN-associated pool of PKC-epsilon is necessary for its concentration at the phagocytic cup and Fc [gamma] receptor (FcgammaR)-dependent membrane fusion
Title: Golgi-Associated Protein Kinase C-? Is Delivered to Phagocytic Cups: Role of Phosphatidylinositol 4-Phosphate.

that PKC-epsilon activation mediates 1) dysfunction of complexes I and III of the respiratory chain, 2) oxidant production, 3) morphological damage to the kidney, and 4) decreases in renal functions after ischemia
Title: Deletion of protein kinase C-? attenuates mitochondrial dysfunction and ameliorates ischemic renal injury.

PKCepsilon is a negative regulator of megakaryopoiesis
Title: PKC-epsilon deficiency alters progenitor cell populations in favor of megakaryopoiesis.

Sstudy provides evidence that PKCepsilon-ALDH2 pathway positively regulates adipocyte differentiation by regulation of PPARgamma transcriptional activity.
Title: PKC-ALDH2 Pathway Plays a Novel Role in Adipocyte Differentiation.

The following Prkce gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkce cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53275	XM_006523833.2 Latest version!	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu23255	NM_011104.3 Latest version!	Mus musculus protein kinase C, epsilon (Prkce), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu53274	XM_017317328.2 Latest version!	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu87132	XM_030249576.1 Latest version!	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu53274	XM_017317328.1	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkce ( XM_006523833.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523896.1 Mus musculus protein kinase C, epsilon (Prkce), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu53275
Clone ID Related Accession (Same CDS sequence)	XM_006523833.2
Accession Version	XM_006523833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kinase C epsilon type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACT CAGACTCCCT TGCCCCTAAA GACAATGAAG AACGAGTGTT CAGGGAGCGT 
ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC AGGCGCAGGG TCCACCAGGT CAATGGCCAC 
AAGTTCATGG CCACCTACTT GCGGCAACCC ACCTACTGCT CCCACTGCAG AGATTTCATC 
TGGGGTGTCA TAGGAAAACA GGGATATCAA TGTCAAGTTT GCACTTGCGT TGTCCACAAG 
CGATGTCATG AGCTCATTAT TACAAAGTGC GCTGGGCTGA AGAAACAGGA AACCCCTGAC 
GAGGTGGGCT CCCAACGGTT CAGCGTCAAC ATGCCCCACA AGTTCGGGAT CCACAACTAC 
AAGGTCCCCA CGTTCTGTGA CCACTGTGGG TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC 
TTGCAGTGTA AAGTCTGCAA AATGAATGTT CACCGGCGAT GTGAGACCAA TGTGGCTCCC 
AACTGTGGGG TAGACGCCAG AGGAATTGCC AAAGTGCTGG CTGACCTTGG TGTTACTCCA 
GACAAAATCA CCAACAGTGG CCAAAGGAGG AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA 
CAGCCGGCTT CTGGAAACTC CCCATCTGAA GACGACCGAT CCAAGTCAGC GCCCACCTCC 
CCTTGTGACC AGGAACTAAA AGAACTTGAA AACAACATTC GGAAGGCCTT GTCATTTGAC 
AACCGAGGAG AGGAGCACCG AGCGTCGTCG GCCACCGATG GCCAGCTGGC AAGCCCCGGA 
GAGAACGGGG AAGTCCGGCC AGGCCAGGCC AAGCGCTTGG GGCTGGATGA GTTCAACTTC 
ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC AAGGTCATGT TGGCGGAACT CAAAGGCAAA 
GATGAAGTCT ACGCTGTGAA GGTCTTGAAG AAGGACGTTA TCCTACAAGA CGATGATGTG 
GACTGCACAA TGACAGAGAA GAGGATTTTG GCTCTGGCTC GGAAACACCC TTATCTAACC 
CAACTCTATT GCTGCTTCCA GACCAAGGAC CGCCTCTTCT TCGTCATGGA ATATGTAAAT 
GGTGGAGACC TCATGTTCCA GATTCAGCGG TCCCGAAAAT TTGATGAGCC TCGTTCTCGG 
TTCTATGCCG CAGAGGTCAC ATCAGCCCTC ATGTTTCTCC ACCAGCACGG AGTGATCTAC 
AGGGATTTGA AACTGGACAA CATCCTTCTA GATGCAGAAG GCCACTGCAA GCTGGCTGAC 
TTTGGGATGT GCAAGGAAGG GATTATGAAT GGTGTGACAA CTACCACCTT CTGTGGGACT 
CCTGACTACA TAGCTCCAGA GATCCTACAG GAGTTGGAGT ACGGCCCCTC AGTGGACTGG 
TGGGCCCTGG GGGTGCTGAT GTACGAGATG ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC 
AACGAGGACG ACTTGTTCGA ATCCATCCTT CATGATGATG TTCTCTATCC TGTCTGGCTT 
AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT TTCATGACCA AGAACCCGCA CAAGCGCCTG 
GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC GCCATCAAGC AACATCCATT CTTCAAGGAG 
ATTGACTGGG TACTGCTGGA GCAGAAGAAA ATCAAGCCCC CCTTCAAGCC GAGAATTAAA 
ACCAAAAGAG ATGTCAATAA CTTTGACCAA GACTTTACGC GGGAAGAGCC AATACTTACA 
CTTGTGGATG AAGCAATCAT TAAGCAGATC AACCAGGAAG AATTCAAAGG CTTCTCCTAC 
TTTGGTGAAG ACCTGATGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523896.1
CDS	16..1839
Translation	MKDSDSLAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDEVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLAAGAESPQPASGNSPSEDDRSKSAPTSPCDQELKELENNIRKALSFDNRGEEHRASSATDGQLASPGENGEVRPGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGIMNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQDFTREEPILTLVDEAIIKQINQEEFKGFSYFGEDLMP

Target ORF information:

RefSeq Version	XM_006523833.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523833.2

ORF Insert Sequence:

ATGAAGGACT CAGACTCCCT TGCCCCTAAA GACAATGAAG AACGAGTGTT CAGGGAGCGT 
ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC AGGCGCAGGG TCCACCAGGT CAATGGCCAC 
AAGTTCATGG CCACCTACTT GCGGCAACCC ACCTACTGCT CCCACTGCAG AGATTTCATC 
TGGGGTGTCA TAGGAAAACA GGGATATCAA TGTCAAGTTT GCACTTGCGT TGTCCACAAG 
CGATGTCATG AGCTCATTAT TACAAAGTGC GCTGGGCTGA AGAAACAGGA AACCCCTGAC 
GAGGTGGGCT CCCAACGGTT CAGCGTCAAC ATGCCCCACA AGTTCGGGAT CCACAACTAC 
AAGGTCCCCA CGTTCTGTGA CCACTGTGGG TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC 
TTGCAGTGTA AAGTCTGCAA AATGAATGTT CACCGGCGAT GTGAGACCAA TGTGGCTCCC 
AACTGTGGGG TAGACGCCAG AGGAATTGCC AAAGTGCTGG CTGACCTTGG TGTTACTCCA 
GACAAAATCA CCAACAGTGG CCAAAGGAGG AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA 
CAGCCGGCTT CTGGAAACTC CCCATCTGAA GACGACCGAT CCAAGTCAGC GCCCACCTCC 
CCTTGTGACC AGGAACTAAA AGAACTTGAA AACAACATTC GGAAGGCCTT GTCATTTGAC 
AACCGAGGAG AGGAGCACCG AGCGTCGTCG GCCACCGATG GCCAGCTGGC AAGCCCCGGA 
GAGAACGGGG AAGTCCGGCC AGGCCAGGCC AAGCGCTTGG GGCTGGATGA GTTCAACTTC 
ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC AAGGTCATGT TGGCGGAACT CAAAGGCAAA 
GATGAAGTCT ACGCTGTGAA GGTCTTGAAG AAGGACGTTA TCCTACAAGA CGATGATGTG 
GACTGCACAA TGACAGAGAA GAGGATTTTG GCTCTGGCTC GGAAACACCC TTATCTAACC 
CAACTCTATT GCTGCTTCCA GACCAAGGAC CGCCTCTTCT TCGTCATGGA ATATGTAAAT 
GGTGGAGACC TCATGTTCCA GATTCAGCGG TCCCGAAAAT TTGATGAGCC TCGTTCTCGG 
TTCTATGCCG CAGAGGTCAC ATCAGCCCTC ATGTTTCTCC ACCAGCACGG AGTGATCTAC 
AGGGATTTGA AACTGGACAA CATCCTTCTA GATGCAGAAG GCCACTGCAA GCTGGCTGAC 
TTTGGGATGT GCAAGGAAGG GATTATGAAT GGTGTGACAA CTACCACCTT CTGTGGGACT 
CCTGACTACA TAGCTCCAGA GATCCTACAG GAGTTGGAGT ACGGCCCCTC AGTGGACTGG 
TGGGCCCTGG GGGTGCTGAT GTACGAGATG ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC 
AACGAGGACG ACTTGTTCGA ATCCATCCTT CATGATGATG TTCTCTATCC TGTCTGGCTT 
AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT TTCATGACCA AGAACCCGCA CAAGCGCCTG 
GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC GCCATCAAGC AACATCCATT CTTCAAGGAG 
ATTGACTGGG TACTGCTGGA GCAGAAGAAA ATCAAGCCCC CCTTCAAGCC GAGAATTAAA 
ACCAAAAGAG ATGTCAATAA CTTTGACCAA GACTTTACGC GGGAAGAGCC AATACTTACA 
CTTGTGGATG AAGCAATCAT TAAGCAGATC AACCAGGAAG AATTCAAAGG CTTCTCCTAC 
TTTGGTGAAG ACCTGATGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkce ( NM_011104.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035234.1 Mus musculus protein kinase C, epsilon (Prkce), mRNA.

Price & Availability↑

CloneID	OMu23255
Clone ID Related Accession (Same CDS sequence)	NM_011104.3
Accession Version	NM_011104.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein kinase C epsilon type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154542.2, CT030001.16, AC154351.2, AC154236.2 and AC096777.1. On Apr 6, 2010 this sequence version replaced NM_011104.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK082438.1, SRR1660815.56488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 11355884 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTAGTGT TCAATGGCCT TCTTAAGATC AAAATCTGCG AGGCGGTGAG CTTGAAGCCC 
ACAGCCTGGT CGCTGCGCCA TGCGGTGGGA CCCCGGCCAC AGACGTTCCT TTTGGACCCC 
TACATTGCCC TTAACGTGGA CGACTCGCGC ATCGGCCAAA CAGCCACCAA GCAAAAGACC 
AACAGCCCGG CCTGGCACGA TGAGTTCGTC ACCGATGTGT GCAATGGGCG CAAGATCGAG 
CTGGCTGTCT TTCACGACGC TCCTATCGGC TACGACGACT TCGTGGCCAA CTGCACCATC 
CAGTTCGAGG AGCTGCTGCA GAATGGGAGC CGTCACTTCG AGGACTGGAT TGACCTGGAG 
CCAGAAGGAA AAGTGTACGT GATCATCGAT CTCTCGGGAT CATCGGGTGA AGCCCCTAAA 
GACAATGAAG AACGAGTGTT CAGGGAGCGT ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC 
AGGCGCAGGG TCCACCAGGT CAATGGCCAC AAGTTCATGG CCACCTACTT GCGGCAACCC 
ACCTACTGCT CCCACTGCAG AGATTTCATC TGGGGTGTCA TAGGAAAACA GGGATATCAA 
TGTCAAGTTT GCACTTGCGT TGTCCACAAG CGATGTCATG AGCTCATTAT TACAAAGTGC 
GCTGGGCTGA AGAAACAGGA AACCCCTGAC GAGGTGGGCT CCCAACGGTT CAGCGTCAAC 
ATGCCCCACA AGTTCGGGAT CCACAACTAC AAGGTCCCCA CGTTCTGTGA CCACTGTGGG 
TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC TTGCAGTGTA AAGTCTGCAA AATGAATGTT 
CACCGGCGAT GTGAGACCAA TGTGGCTCCC AACTGTGGGG TAGACGCCAG AGGAATTGCC 
AAAGTGCTGG CTGACCTTGG TGTTACTCCA GACAAAATCA CCAACAGTGG CCAAAGGAGG 
AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA CAGCCGGCTT CTGGAAACTC CCCATCTGAA 
GACGACCGAT CCAAGTCAGC GCCCACCTCC CCTTGTGACC AGGAACTAAA AGAACTTGAA 
AACAACATTC GGAAGGCCTT GTCATTTGAC AACCGAGGAG AGGAGCACCG AGCGTCGTCG 
GCCACCGATG GCCAGCTGGC AAGCCCCGGA GAGAACGGGG AAGTCCGGCC AGGCCAGGCC 
AAGCGCTTGG GGCTGGATGA GTTCAACTTC ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC 
AAGGTCATGT TGGCGGAACT CAAAGGCAAA GATGAAGTCT ACGCTGTGAA GGTCTTGAAG 
AAGGACGTTA TCCTACAAGA CGATGATGTG GACTGCACAA TGACAGAGAA GAGGATTTTG 
GCTCTGGCTC GGAAACACCC TTATCTAACC CAACTCTATT GCTGCTTCCA GACCAAGGAC 
CGCCTCTTCT TCGTCATGGA ATATGTAAAT GGTGGAGACC TCATGTTCCA GATTCAGCGG 
TCCCGAAAAT TTGATGAGCC TCGTTCTCGG TTCTATGCCG CAGAGGTCAC ATCAGCCCTC 
ATGTTTCTCC ACCAGCACGG AGTGATCTAC AGGGATTTGA AACTGGACAA CATCCTTCTA 
GATGCAGAAG GCCACTGCAA GCTGGCTGAC TTTGGGATGT GCAAGGAAGG GATTATGAAT 
GGTGTGACAA CTACCACCTT CTGTGGGACT CCTGACTACA TAGCTCCAGA GATCCTACAG 
GAGTTGGAGT ACGGCCCCTC AGTGGACTGG TGGGCCCTGG GGGTGCTGAT GTACGAGATG 
ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC AACGAGGACG ACTTGTTCGA ATCCATCCTT 
CATGATGATG TTCTCTATCC TGTCTGGCTT AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT 
TTCATGACCA AGAACCCGCA CAAGCGCCTG GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC 
GCCATCAAGC AACATCCATT CTTCAAGGAG ATTGACTGGG TACTGCTGGA GCAGAAGAAA 
ATCAAGCCCC CCTTCAAGCC GAGAATTAAA ACCAAAAGAG ATGTCAATAA CTTTGACCAA 
GACTTTACGC GGGAAGAGCC AATACTTACA CTTGTGGATG AAGCAATCAT TAAGCAGATC 
AACCAGGAAG AATTCAAAGG CTTCTCCTAC TTTGGTGAAG ACCTGATGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035234.1
CDS	809..3022
Translation	MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGKVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDEVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLAAGAESPQPASGNSPSEDDRSKSAPTSPCDQELKELENNIRKALSFDNRGEEHRASSATDGQLASPGENGEVRPGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGIMNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQDFTREEPILTLVDEAIIKQINQEEFKGFSYFGEDLMP

Target ORF information:

RefSeq Version	NM_011104.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, epsilon (Prkce), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011104.3

ORF Insert Sequence:

ATGGTAGTGT TCAATGGCCT TCTTAAGATC AAAATCTGCG AGGCGGTGAG CTTGAAGCCC 
ACAGCCTGGT CGCTGCGCCA TGCGGTGGGA CCCCGGCCAC AGACGTTCCT TTTGGACCCC 
TACATTGCCC TTAACGTGGA CGACTCGCGC ATCGGCCAAA CAGCCACCAA GCAAAAGACC 
AACAGCCCGG CCTGGCACGA TGAGTTCGTC ACCGATGTGT GCAATGGGCG CAAGATCGAG 
CTGGCTGTCT TTCACGACGC TCCTATCGGC TACGACGACT TCGTGGCCAA CTGCACCATC 
CAGTTCGAGG AGCTGCTGCA GAATGGGAGC CGTCACTTCG AGGACTGGAT TGACCTGGAG 
CCAGAAGGAA AAGTGTACGT GATCATCGAT CTCTCGGGAT CATCGGGTGA AGCCCCTAAA 
GACAATGAAG AACGAGTGTT CAGGGAGCGT ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC 
AGGCGCAGGG TCCACCAGGT CAATGGCCAC AAGTTCATGG CCACCTACTT GCGGCAACCC 
ACCTACTGCT CCCACTGCAG AGATTTCATC TGGGGTGTCA TAGGAAAACA GGGATATCAA 
TGTCAAGTTT GCACTTGCGT TGTCCACAAG CGATGTCATG AGCTCATTAT TACAAAGTGC 
GCTGGGCTGA AGAAACAGGA AACCCCTGAC GAGGTGGGCT CCCAACGGTT CAGCGTCAAC 
ATGCCCCACA AGTTCGGGAT CCACAACTAC AAGGTCCCCA CGTTCTGTGA CCACTGTGGG 
TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC TTGCAGTGTA AAGTCTGCAA AATGAATGTT 
CACCGGCGAT GTGAGACCAA TGTGGCTCCC AACTGTGGGG TAGACGCCAG AGGAATTGCC 
AAAGTGCTGG CTGACCTTGG TGTTACTCCA GACAAAATCA CCAACAGTGG CCAAAGGAGG 
AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA CAGCCGGCTT CTGGAAACTC CCCATCTGAA 
GACGACCGAT CCAAGTCAGC GCCCACCTCC CCTTGTGACC AGGAACTAAA AGAACTTGAA 
AACAACATTC GGAAGGCCTT GTCATTTGAC AACCGAGGAG AGGAGCACCG AGCGTCGTCG 
GCCACCGATG GCCAGCTGGC AAGCCCCGGA GAGAACGGGG AAGTCCGGCC AGGCCAGGCC 
AAGCGCTTGG GGCTGGATGA GTTCAACTTC ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC 
AAGGTCATGT TGGCGGAACT CAAAGGCAAA GATGAAGTCT ACGCTGTGAA GGTCTTGAAG 
AAGGACGTTA TCCTACAAGA CGATGATGTG GACTGCACAA TGACAGAGAA GAGGATTTTG 
GCTCTGGCTC GGAAACACCC TTATCTAACC CAACTCTATT GCTGCTTCCA GACCAAGGAC 
CGCCTCTTCT TCGTCATGGA ATATGTAAAT GGTGGAGACC TCATGTTCCA GATTCAGCGG 
TCCCGAAAAT TTGATGAGCC TCGTTCTCGG TTCTATGCCG CAGAGGTCAC ATCAGCCCTC 
ATGTTTCTCC ACCAGCACGG AGTGATCTAC AGGGATTTGA AACTGGACAA CATCCTTCTA 
GATGCAGAAG GCCACTGCAA GCTGGCTGAC TTTGGGATGT GCAAGGAAGG GATTATGAAT 
GGTGTGACAA CTACCACCTT CTGTGGGACT CCTGACTACA TAGCTCCAGA GATCCTACAG 
GAGTTGGAGT ACGGCCCCTC AGTGGACTGG TGGGCCCTGG GGGTGCTGAT GTACGAGATG 
ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC AACGAGGACG ACTTGTTCGA ATCCATCCTT 
CATGATGATG TTCTCTATCC TGTCTGGCTT AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT 
TTCATGACCA AGAACCCGCA CAAGCGCCTG GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC 
GCCATCAAGC AACATCCATT CTTCAAGGAG ATTGACTGGG TACTGCTGGA GCAGAAGAAA 
ATCAAGCCCC CCTTCAAGCC GAGAATTAAA ACCAAAAGAG ATGTCAATAA CTTTGACCAA 
GACTTTACGC GGGAAGAGCC AATACTTACA CTTGTGGATG AAGCAATCAT TAAGCAGATC 
AACCAGGAAG AATTCAAAGG CTTCTCCTAC TTTGGTGAAG ACCTGATGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkce ( XM_017317328.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172817.1 Mus musculus protein kinase C, epsilon (Prkce), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53274
Clone ID Related Accession (Same CDS sequence)	XM_017317328.1 , XM_017317328.2
Accession Version	XM_017317328.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein kinase C epsilon type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTGT TCAATGGCCT TCTTAAGATC AAAATCTGCG AGGCGGTGAG CTTGAAGCCC 
ACAGCCTGGT CGCTGCGCCA TGCGGTGGGA CCCCGGCCAC AGACGTTCCT TTTGGACCCC 
TACATTGCCC TTAACGTGGA CGACTCGCGC ATCGGCCAAA CAGCCACCAA GCAAAAGACC 
AACAGCCCGG CCTGGCACGA TGAGTTCGTC ACCGATGTGT GCAATGGGCG CAAGATCGAG 
CTGGCTGTCT TTCACGACGC TCCTATCGGC TACGACGACT TCGTGGCCAA CTGCACCATC 
CAGTTCGAGG AGCTGCTGCA GAATGGGAGC CGTCACTTCG AGGACTGGAT TGACCTGGAG 
CCAGAAGGAA AAGTGTACGT GATCATCGAT CTCTCGGGAT CATCGGGTGA AGCCCCTAAA 
GACAATGAAG AACGAGTGTT CAGGGAGCGT ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC 
AGGCGCAGGG TCCACCAGGT CAATGGCCAC AAGTTCATGG CCACCTACTT GCGGCAACCC 
ACCTACTGCT CCCACTGCAG AGATTTCATC TGGGGTGTCA TAGGAAAACA GGGATATCAA 
TGTCAAGTTT GCACTTGCGT TGTCCACAAG CGATGTCATG AGCTCATTAT TACAAAGTGC 
GCTGGGCTGA AGAAACAGGA AACCCCTGAC GAGGTGGGCT CCCAACGGTT CAGCGTCAAC 
ATGCCCCACA AGTTCGGGAT CCACAACTAC AAGGTCCCCA CGTTCTGTGA CCACTGTGGG 
TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC TTGCAGTGTA AAGTCTGCAA AATGAATGTT 
CACCGGCGAT GTGAGACCAA TGTGGCTCCC AACTGTGGGG TAGACGCCAG AGGAATTGCC 
AAAGTGCTGG CTGACCTTGG TGTTACTCCA GACAAAATCA CCAACAGTGG CCAAAGGAGG 
AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA CAGCCGGCTT CTGGAAACTC CCCATCTGAA 
GACGACCGAT CCAAGTCAGC GCCCACCTCC CCTTGTGACC AGGAACTAAA AGAACTTGAA 
AACAACATTC GGAAGGCCTT GTCATTTGAC AACCGAGGAG AGGAGCACCG AGCGTCGTCG 
GCCACCGATG GCCAGCTGGC AAGCCCCGGA GAGAACGGGG AAGTCCGGCC AGGCCAGGCC 
AAGCGCTTGG GGCTGGATGA GTTCAACTTC ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC 
AAGGTCATGT TGGCGGAACT CAAAGGCAAA GATGAAGTCT ACGCTGTGAA GGTCTTGAAG 
AAGGACGTTA TCCTACAAGA CGATGATGTG GACTGCACAA TGACAGAGAA GAGGATTTTG 
GCTCTGGCTC GGAAACACCC TTATCTAACC CAACTCTATT GCTGCTTCCA GACCAAGGAC 
CGCCTCTTCT TCGTCATGGA ATATGTAAAT GGTGGAGACC TCATGTTCCA GATTCAGCGG 
TCCCGAAAAT TTGATGAGCC TCGTTCTCGG TTCTATGCCG CAGAGGTCAC ATCAGCCCTC 
ATGTTTCTCC ACCAGCACGG AGTGATCTAC AGGGATTTGA AACTGGACAA CATCCTTCTA 
GATGCAGAAG GCCACTGCAA GCTGGCTGAC TTTGGGATGT GCAAGGAAGG GATTATGAAT 
GGTGTGACAA CTACCACCTT CTGTGGGACT CCTGACTACA TAGCTCCAGA GATCCTACAG 
GAGTTGGAGT ACGGCCCCTC AGTGGACTGG TGGGCCCTGG GGGTGCTGAT GTACGAGATG 
ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC AACGAGGACG ACTTGTTCGA ATCCATCCTT 
CATGATGATG TTCTCTATCC TGTCTGGCTT AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT 
TTCATGACCA AGAACCCGCA CAAGCGCCTG GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC 
GCCATCAAGC AACATCCATT CTTCAAGGAG ATTGACTGGG TACTGCTGGA GCAGAAGAAA 
ATCAAGCCCC CCTTCAAGCC GAGAATTTTT GCTTCAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172817.1
CDS	1040..3121
Translation	MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGKVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDEVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLAAGAESPQPASGNSPSEDDRSKSAPTSPCDQELKELENNIRKALSFDNRGEEHRASSATDGQLASPGENGEVRPGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGIMNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIFASV

Target ORF information:

RefSeq Version	XM_017317328.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317328.1

ORF Insert Sequence:

ATGGTAGTGT TCAATGGCCT TCTTAAGATC AAAATCTGCG AGGCGGTGAG CTTGAAGCCC 
ACAGCCTGGT CGCTGCGCCA TGCGGTGGGA CCCCGGCCAC AGACGTTCCT TTTGGACCCC 
TACATTGCCC TTAACGTGGA CGACTCGCGC ATCGGCCAAA CAGCCACCAA GCAAAAGACC 
AACAGCCCGG CCTGGCACGA TGAGTTCGTC ACCGATGTGT GCAATGGGCG CAAGATCGAG 
CTGGCTGTCT TTCACGACGC TCCTATCGGC TACGACGACT TCGTGGCCAA CTGCACCATC 
CAGTTCGAGG AGCTGCTGCA GAATGGGAGC CGTCACTTCG AGGACTGGAT TGACCTGGAG 
CCAGAAGGAA AAGTGTACGT GATCATCGAT CTCTCGGGAT CATCGGGTGA AGCCCCTAAA 
GACAATGAAG AACGAGTGTT CAGGGAGCGT ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC 
AGGCGCAGGG TCCACCAGGT CAATGGCCAC AAGTTCATGG CCACCTACTT GCGGCAACCC 
ACCTACTGCT CCCACTGCAG AGATTTCATC TGGGGTGTCA TAGGAAAACA GGGATATCAA 
TGTCAAGTTT GCACTTGCGT TGTCCACAAG CGATGTCATG AGCTCATTAT TACAAAGTGC 
GCTGGGCTGA AGAAACAGGA AACCCCTGAC GAGGTGGGCT CCCAACGGTT CAGCGTCAAC 
ATGCCCCACA AGTTCGGGAT CCACAACTAC AAGGTCCCCA CGTTCTGTGA CCACTGTGGG 
TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC TTGCAGTGTA AAGTCTGCAA AATGAATGTT 
CACCGGCGAT GTGAGACCAA TGTGGCTCCC AACTGTGGGG TAGACGCCAG AGGAATTGCC 
AAAGTGCTGG CTGACCTTGG TGTTACTCCA GACAAAATCA CCAACAGTGG CCAAAGGAGG 
AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA CAGCCGGCTT CTGGAAACTC CCCATCTGAA 
GACGACCGAT CCAAGTCAGC GCCCACCTCC CCTTGTGACC AGGAACTAAA AGAACTTGAA 
AACAACATTC GGAAGGCCTT GTCATTTGAC AACCGAGGAG AGGAGCACCG AGCGTCGTCG 
GCCACCGATG GCCAGCTGGC AAGCCCCGGA GAGAACGGGG AAGTCCGGCC AGGCCAGGCC 
AAGCGCTTGG GGCTGGATGA GTTCAACTTC ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC 
AAGGTCATGT TGGCGGAACT CAAAGGCAAA GATGAAGTCT ACGCTGTGAA GGTCTTGAAG 
AAGGACGTTA TCCTACAAGA CGATGATGTG GACTGCACAA TGACAGAGAA GAGGATTTTG 
GCTCTGGCTC GGAAACACCC TTATCTAACC CAACTCTATT GCTGCTTCCA GACCAAGGAC 
CGCCTCTTCT TCGTCATGGA ATATGTAAAT GGTGGAGACC TCATGTTCCA GATTCAGCGG 
TCCCGAAAAT TTGATGAGCC TCGTTCTCGG TTCTATGCCG CAGAGGTCAC ATCAGCCCTC 
ATGTTTCTCC ACCAGCACGG AGTGATCTAC AGGGATTTGA AACTGGACAA CATCCTTCTA 
GATGCAGAAG GCCACTGCAA GCTGGCTGAC TTTGGGATGT GCAAGGAAGG GATTATGAAT 
GGTGTGACAA CTACCACCTT CTGTGGGACT CCTGACTACA TAGCTCCAGA GATCCTACAG 
GAGTTGGAGT ACGGCCCCTC AGTGGACTGG TGGGCCCTGG GGGTGCTGAT GTACGAGATG 
ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC AACGAGGACG ACTTGTTCGA ATCCATCCTT 
CATGATGATG TTCTCTATCC TGTCTGGCTT AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT 
TTCATGACCA AGAACCCGCA CAAGCGCCTG GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC 
GCCATCAAGC AACATCCATT CTTCAAGGAG ATTGACTGGG TACTGCTGGA GCAGAAGAAA 
ATCAAGCCCC CCTTCAAGCC GAGAATTTTT GCTTCAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkce ( XM_017317328.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172817.1 Mus musculus protein kinase C, epsilon (Prkce), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53274
Clone ID Related Accession (Same CDS sequence)	XM_017317328.1 , XM_017317328.2
Accession Version	XM_017317328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kinase C epsilon type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTGT TCAATGGCCT TCTTAAGATC AAAATCTGCG AGGCGGTGAG CTTGAAGCCC 
ACAGCCTGGT CGCTGCGCCA TGCGGTGGGA CCCCGGCCAC AGACGTTCCT TTTGGACCCC 
TACATTGCCC TTAACGTGGA CGACTCGCGC ATCGGCCAAA CAGCCACCAA GCAAAAGACC 
AACAGCCCGG CCTGGCACGA TGAGTTCGTC ACCGATGTGT GCAATGGGCG CAAGATCGAG 
CTGGCTGTCT TTCACGACGC TCCTATCGGC TACGACGACT TCGTGGCCAA CTGCACCATC 
CAGTTCGAGG AGCTGCTGCA GAATGGGAGC CGTCACTTCG AGGACTGGAT TGACCTGGAG 
CCAGAAGGAA AAGTGTACGT GATCATCGAT CTCTCGGGAT CATCGGGTGA AGCCCCTAAA 
GACAATGAAG AACGAGTGTT CAGGGAGCGT ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC 
AGGCGCAGGG TCCACCAGGT CAATGGCCAC AAGTTCATGG CCACCTACTT GCGGCAACCC 
ACCTACTGCT CCCACTGCAG AGATTTCATC TGGGGTGTCA TAGGAAAACA GGGATATCAA 
TGTCAAGTTT GCACTTGCGT TGTCCACAAG CGATGTCATG AGCTCATTAT TACAAAGTGC 
GCTGGGCTGA AGAAACAGGA AACCCCTGAC GAGGTGGGCT CCCAACGGTT CAGCGTCAAC 
ATGCCCCACA AGTTCGGGAT CCACAACTAC AAGGTCCCCA CGTTCTGTGA CCACTGTGGG 
TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC TTGCAGTGTA AAGTCTGCAA AATGAATGTT 
CACCGGCGAT GTGAGACCAA TGTGGCTCCC AACTGTGGGG TAGACGCCAG AGGAATTGCC 
AAAGTGCTGG CTGACCTTGG TGTTACTCCA GACAAAATCA CCAACAGTGG CCAAAGGAGG 
AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA CAGCCGGCTT CTGGAAACTC CCCATCTGAA 
GACGACCGAT CCAAGTCAGC GCCCACCTCC CCTTGTGACC AGGAACTAAA AGAACTTGAA 
AACAACATTC GGAAGGCCTT GTCATTTGAC AACCGAGGAG AGGAGCACCG AGCGTCGTCG 
GCCACCGATG GCCAGCTGGC AAGCCCCGGA GAGAACGGGG AAGTCCGGCC AGGCCAGGCC 
AAGCGCTTGG GGCTGGATGA GTTCAACTTC ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC 
AAGGTCATGT TGGCGGAACT CAAAGGCAAA GATGAAGTCT ACGCTGTGAA GGTCTTGAAG 
AAGGACGTTA TCCTACAAGA CGATGATGTG GACTGCACAA TGACAGAGAA GAGGATTTTG 
GCTCTGGCTC GGAAACACCC TTATCTAACC CAACTCTATT GCTGCTTCCA GACCAAGGAC 
CGCCTCTTCT TCGTCATGGA ATATGTAAAT GGTGGAGACC TCATGTTCCA GATTCAGCGG 
TCCCGAAAAT TTGATGAGCC TCGTTCTCGG TTCTATGCCG CAGAGGTCAC ATCAGCCCTC 
ATGTTTCTCC ACCAGCACGG AGTGATCTAC AGGGATTTGA AACTGGACAA CATCCTTCTA 
GATGCAGAAG GCCACTGCAA GCTGGCTGAC TTTGGGATGT GCAAGGAAGG GATTATGAAT 
GGTGTGACAA CTACCACCTT CTGTGGGACT CCTGACTACA TAGCTCCAGA GATCCTACAG 
GAGTTGGAGT ACGGCCCCTC AGTGGACTGG TGGGCCCTGG GGGTGCTGAT GTACGAGATG 
ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC AACGAGGACG ACTTGTTCGA ATCCATCCTT 
CATGATGATG TTCTCTATCC TGTCTGGCTT AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT 
TTCATGACCA AGAACCCGCA CAAGCGCCTG GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC 
GCCATCAAGC AACATCCATT CTTCAAGGAG ATTGACTGGG TACTGCTGGA GCAGAAGAAA 
ATCAAGCCCC CCTTCAAGCC GAGAATTTTT GCTTCAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172817.1
CDS	782..2863
Translation	MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGKVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDEVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLAAGAESPQPASGNSPSEDDRSKSAPTSPCDQELKELENNIRKALSFDNRGEEHRASSATDGQLASPGENGEVRPGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGIMNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIFASV

Target ORF information:

RefSeq Version	XM_017317328.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317328.2

ORF Insert Sequence:

ATGGTAGTGT TCAATGGCCT TCTTAAGATC AAAATCTGCG AGGCGGTGAG CTTGAAGCCC 
ACAGCCTGGT CGCTGCGCCA TGCGGTGGGA CCCCGGCCAC AGACGTTCCT TTTGGACCCC 
TACATTGCCC TTAACGTGGA CGACTCGCGC ATCGGCCAAA CAGCCACCAA GCAAAAGACC 
AACAGCCCGG CCTGGCACGA TGAGTTCGTC ACCGATGTGT GCAATGGGCG CAAGATCGAG 
CTGGCTGTCT TTCACGACGC TCCTATCGGC TACGACGACT TCGTGGCCAA CTGCACCATC 
CAGTTCGAGG AGCTGCTGCA GAATGGGAGC CGTCACTTCG AGGACTGGAT TGACCTGGAG 
CCAGAAGGAA AAGTGTACGT GATCATCGAT CTCTCGGGAT CATCGGGTGA AGCCCCTAAA 
GACAATGAAG AACGAGTGTT CAGGGAGCGT ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC 
AGGCGCAGGG TCCACCAGGT CAATGGCCAC AAGTTCATGG CCACCTACTT GCGGCAACCC 
ACCTACTGCT CCCACTGCAG AGATTTCATC TGGGGTGTCA TAGGAAAACA GGGATATCAA 
TGTCAAGTTT GCACTTGCGT TGTCCACAAG CGATGTCATG AGCTCATTAT TACAAAGTGC 
GCTGGGCTGA AGAAACAGGA AACCCCTGAC GAGGTGGGCT CCCAACGGTT CAGCGTCAAC 
ATGCCCCACA AGTTCGGGAT CCACAACTAC AAGGTCCCCA CGTTCTGTGA CCACTGTGGG 
TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC TTGCAGTGTA AAGTCTGCAA AATGAATGTT 
CACCGGCGAT GTGAGACCAA TGTGGCTCCC AACTGTGGGG TAGACGCCAG AGGAATTGCC 
AAAGTGCTGG CTGACCTTGG TGTTACTCCA GACAAAATCA CCAACAGTGG CCAAAGGAGG 
AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA CAGCCGGCTT CTGGAAACTC CCCATCTGAA 
GACGACCGAT CCAAGTCAGC GCCCACCTCC CCTTGTGACC AGGAACTAAA AGAACTTGAA 
AACAACATTC GGAAGGCCTT GTCATTTGAC AACCGAGGAG AGGAGCACCG AGCGTCGTCG 
GCCACCGATG GCCAGCTGGC AAGCCCCGGA GAGAACGGGG AAGTCCGGCC AGGCCAGGCC 
AAGCGCTTGG GGCTGGATGA GTTCAACTTC ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC 
AAGGTCATGT TGGCGGAACT CAAAGGCAAA GATGAAGTCT ACGCTGTGAA GGTCTTGAAG 
AAGGACGTTA TCCTACAAGA CGATGATGTG GACTGCACAA TGACAGAGAA GAGGATTTTG 
GCTCTGGCTC GGAAACACCC TTATCTAACC CAACTCTATT GCTGCTTCCA GACCAAGGAC 
CGCCTCTTCT TCGTCATGGA ATATGTAAAT GGTGGAGACC TCATGTTCCA GATTCAGCGG 
TCCCGAAAAT TTGATGAGCC TCGTTCTCGG TTCTATGCCG CAGAGGTCAC ATCAGCCCTC 
ATGTTTCTCC ACCAGCACGG AGTGATCTAC AGGGATTTGA AACTGGACAA CATCCTTCTA 
GATGCAGAAG GCCACTGCAA GCTGGCTGAC TTTGGGATGT GCAAGGAAGG GATTATGAAT 
GGTGTGACAA CTACCACCTT CTGTGGGACT CCTGACTACA TAGCTCCAGA GATCCTACAG 
GAGTTGGAGT ACGGCCCCTC AGTGGACTGG TGGGCCCTGG GGGTGCTGAT GTACGAGATG 
ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC AACGAGGACG ACTTGTTCGA ATCCATCCTT 
CATGATGATG TTCTCTATCC TGTCTGGCTT AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT 
TTCATGACCA AGAACCCGCA CAAGCGCCTG GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC 
GCCATCAAGC AACATCCATT CTTCAAGGAG ATTGACTGGG TACTGCTGGA GCAGAAGAAA 
ATCAAGCCCC CCTTCAAGCC GAGAATTTTT GCTTCAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkce ( XM_030249576.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105436.1 Mus musculus protein kinase C, epsilon (Prkce), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu87132
Clone ID Related Accession (Same CDS sequence)	XM_030249576.1
Accession Version	XM_030249576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kinase C epsilon type isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC AGGCGCAGGG TCCACCAGGT CAATGGCCAC 
AAGTTCATGG CCACCTACTT GCGGCAACCC ACCTACTGCT CCCACTGCAG AGATTTCATC 
TGGGGTGTCA TAGGAAAACA GGGATATCAA TGTCAAGTTT GCACTTGCGT TGTCCACAAG 
CGATGTCATG AGCTCATTAT TACAAAGTGC GCTGGGCTGA AGAAACAGGA AACCCCTGAC 
GAGGTGGGCT CCCAACGGTT CAGCGTCAAC ATGCCCCACA AGTTCGGGAT CCACAACTAC 
AAGGTCCCCA CGTTCTGTGA CCACTGTGGG TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC 
TTGCAGTGTA AAGTCTGCAA AATGAATGTT CACCGGCGAT GTGAGACCAA TGTGGCTCCC 
AACTGTGGGG TAGACGCCAG AGGAATTGCC AAAGTGCTGG CTGACCTTGG TGTTACTCCA 
GACAAAATCA CCAACAGTGG CCAAAGGAGG AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA 
CAGCCGGCTT CTGGAAACTC CCCATCTGAA GACGACCGAT CCAAGTCAGC GCCCACCTCC 
CCTTGTGACC AGGAACTAAA AGAACTTGAA AACAACATTC GGAAGGCCTT GTCATTTGAC 
AACCGAGGAG AGGAGCACCG AGCGTCGTCG GCCACCGATG GCCAGCTGGC AAGCCCCGGA 
GAGAACGGGG AAGTCCGGCC AGGCCAGGCC AAGCGCTTGG GGCTGGATGA GTTCAACTTC 
ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC AAGGTCATGT TGGCGGAACT CAAAGGCAAA 
GATGAAGTCT ACGCTGTGAA GGTCTTGAAG AAGGACGTTA TCCTACAAGA CGATGATGTG 
GACTGCACAA TGACAGAGAA GAGGATTTTG GCTCTGGCTC GGAAACACCC TTATCTAACC 
CAACTCTATT GCTGCTTCCA GACCAAGGAC CGCCTCTTCT TCGTCATGGA ATATGTAAAT 
GGTGGAGACC TCATGTTCCA GATTCAGCGG TCCCGAAAAT TTGATGAGCC TCGTTCTCGG 
TTCTATGCCG CAGAGGTCAC ATCAGCCCTC ATGTTTCTCC ACCAGCACGG AGTGATCTAC 
AGGGATTTGA AACTGGACAA CATCCTTCTA GATGCAGAAG GCCACTGCAA GCTGGCTGAC 
TTTGGGATGT GCAAGGAAGG GATTATGAAT GGTGTGACAA CTACCACCTT CTGTGGGACT 
CCTGACTACA TAGCTCCAGA GATCCTACAG GAGTTGGAGT ACGGCCCCTC AGTGGACTGG 
TGGGCCCTGG GGGTGCTGAT GTACGAGATG ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC 
AACGAGGACG ACTTGTTCGA ATCCATCCTT CATGATGATG TTCTCTATCC TGTCTGGCTT 
AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT TTCATGACCA AGAACCCGCA CAAGCGCCTG 
GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC GCCATCAAGC AACATCCATT CTTCAAGGAG 
ATTGACTGGG TACTGCTGGA GCAGAAGAAA ATCAAGCCCC CCTTCAAGCC GAGAATTAAA 
ACCAAAAGAG ATGTCAATAA CTTTGACCAA GACTTTACGC GGGAAGAGCC AATACTTACA 
CTTGTGGATG AAGCAATCAT TAAGCAGATC AACCAGGAAG AATTCAAAGG CTTCTCCTAC 
TTTGGTGAAG ACCTGATGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105436.1
CDS	196..1959
Translation	MRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDEVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLAAGAESPQPASGNSPSEDDRSKSAPTSPCDQELKELENNIRKALSFDNRGEEHRASSATDGQLASPGENGEVRPGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGIMNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQDFTREEPILTLVDEAIIKQINQEEFKGFSYFGEDLMP

Target ORF information:

RefSeq Version	XM_030249576.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase C, epsilon (Prkce), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249576.1

ORF Insert Sequence:

ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC AGGCGCAGGG TCCACCAGGT CAATGGCCAC 
AAGTTCATGG CCACCTACTT GCGGCAACCC ACCTACTGCT CCCACTGCAG AGATTTCATC 
TGGGGTGTCA TAGGAAAACA GGGATATCAA TGTCAAGTTT GCACTTGCGT TGTCCACAAG 
CGATGTCATG AGCTCATTAT TACAAAGTGC GCTGGGCTGA AGAAACAGGA AACCCCTGAC 
GAGGTGGGCT CCCAACGGTT CAGCGTCAAC ATGCCCCACA AGTTCGGGAT CCACAACTAC 
AAGGTCCCCA CGTTCTGTGA CCACTGTGGG TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC 
TTGCAGTGTA AAGTCTGCAA AATGAATGTT CACCGGCGAT GTGAGACCAA TGTGGCTCCC 
AACTGTGGGG TAGACGCCAG AGGAATTGCC AAAGTGCTGG CTGACCTTGG TGTTACTCCA 
GACAAAATCA CCAACAGTGG CCAAAGGAGG AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA 
CAGCCGGCTT CTGGAAACTC CCCATCTGAA GACGACCGAT CCAAGTCAGC GCCCACCTCC 
CCTTGTGACC AGGAACTAAA AGAACTTGAA AACAACATTC GGAAGGCCTT GTCATTTGAC 
AACCGAGGAG AGGAGCACCG AGCGTCGTCG GCCACCGATG GCCAGCTGGC AAGCCCCGGA 
GAGAACGGGG AAGTCCGGCC AGGCCAGGCC AAGCGCTTGG GGCTGGATGA GTTCAACTTC 
ATCAAGGTGT TGGGCAAAGG CAGCTTTGGC AAGGTCATGT TGGCGGAACT CAAAGGCAAA 
GATGAAGTCT ACGCTGTGAA GGTCTTGAAG AAGGACGTTA TCCTACAAGA CGATGATGTG 
GACTGCACAA TGACAGAGAA GAGGATTTTG GCTCTGGCTC GGAAACACCC TTATCTAACC 
CAACTCTATT GCTGCTTCCA GACCAAGGAC CGCCTCTTCT TCGTCATGGA ATATGTAAAT 
GGTGGAGACC TCATGTTCCA GATTCAGCGG TCCCGAAAAT TTGATGAGCC TCGTTCTCGG 
TTCTATGCCG CAGAGGTCAC ATCAGCCCTC ATGTTTCTCC ACCAGCACGG AGTGATCTAC 
AGGGATTTGA AACTGGACAA CATCCTTCTA GATGCAGAAG GCCACTGCAA GCTGGCTGAC 
TTTGGGATGT GCAAGGAAGG GATTATGAAT GGTGTGACAA CTACCACCTT CTGTGGGACT 
CCTGACTACA TAGCTCCAGA GATCCTACAG GAGTTGGAGT ACGGCCCCTC AGTGGACTGG 
TGGGCCCTGG GGGTGCTGAT GTACGAGATG ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC 
AACGAGGACG ACTTGTTCGA ATCCATCCTT CATGATGATG TTCTCTATCC TGTCTGGCTT 
AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT TTCATGACCA AGAACCCGCA CAAGCGCCTG 
GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC GCCATCAAGC AACATCCATT CTTCAAGGAG 
ATTGACTGGG TACTGCTGGA GCAGAAGAAA ATCAAGCCCC CCTTCAAGCC GAGAATTAAA 
ACCAAAAGAG ATGTCAATAA CTTTGACCAA GACTTTACGC GGGAAGAGCC AATACTTACA 
CTTGTGGATG AAGCAATCAT TAAGCAGATC AACCAGGAAG AATTCAAAGG CTTCTCCTAC 
TTTGGTGAAG ACCTGATGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

O-GlcNAc on PKC? Inhibits the FGF4-PKC?-MEK-ERK1/2 Pathway via Inhibition of PKC? Phosphorylation in Mouse Embryonic Stem Cells.
Stem cell reports10(1)272-286(2018 Jan)
Miura T,Kume M,Kawamura T,Yamamoto K,Hamakubo T,Nishihara S

PKC-epsilon deficiency alters progenitor cell populations in favor of megakaryopoiesis.
PloS one12(8)e0182867(2017)
Kostyak JC,Liverani E,Kunapuli SP

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Prkce cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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