Prkaca cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkaca
Entrez Gene ID	18747
Full Name	protein kinase, cAMP dependent, catalytic, alpha
Synonyms	PKCD,Pkaca
General protein information	Preferred Names protein kinase, cAMP dependent, catalytic, alpha Names cAMP-dependent protein kinase catalytic subunit alpha PKA C-alpha sperm cAMP-dependent protein kinase catalytic subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C2
Summary	This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008854.5	NP_032880.1	cAMP-dependent protein kinase catalytic subunit alpha isoform 1
NM_001277898.1	NP_001264827.1	cAMP-dependent protein kinase catalytic subunit alpha isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkaca	NP_032880.1
Danio rerio (zebrafish)	prkacab	NP_001003470.1
Kluyveromyces lactis	KLLA0D03190g	XP_453207.1
Homo sapiens (human)	PRKACA	NP_002721.1
Bos taurus (cattle)	PRKACA	NP_777009.1
Schizosaccharomyces pombe (fission yeast)	pka1	NP_595159.1
Xenopus tropicalis (tropical clawed frog)	prkaca	NP_001016403.1
Pan troglodytes (chimpanzee)	PRKACA	XP_001171437.1
Saccharomyces cerevisiae (baker's yeast)	TPK2	NP_015121.1
Eremothecium gossypii	AGOS_AFL090W	NP_985458.1
Rattus norvegicus (Norway rat)	Prkaca	NP_001094392.1
Canis lupus familiaris (dog)	PRKACA	NP_001003032.1

Gene Ontology
Bibliography

Related articles in PubMed

Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility.
Nolan MA, Babcock DF, Wennemuth G, Brown W, Burton KA, McKnight GS
Proceedings of the National Academy of Sciences of the United States of America101(37)13483-8(2004 Sep)

PKA: a portrait of protein kinase dynamics.
Taylor SS, Yang J, Wu J, Haste NM, Radzio-Andzelm E, Anand G
Biochimica et biophysica acta1697(1-2)259-69(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that the upregulation of mitochondrial respiratory chain proteins played a partial role in the protection of PKA/CREB signaling.
Title: Protein Kinase A/CREB Signaling Prevents Adriamycin-Induced Podocyte Apoptosis via Upregulation of Mitochondrial Respiratory Chain Complexes.

Generation of the Dnajb1-Prkaca fusion gene in wild-type mice to be sufficient to initiate formation of tumors that have many features of human fibrolamellar hepatocellular carcinonma.
Title: CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates?That the Dnajb1-Prkaca Gene Fusion Is Sufficient?to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma.

study identifies a new role of Dual-AKAP1 in regulating mitochondrial trafficking through Miro-2, and supports a model in which PINK1 and mitochondrial PKA participate in a similar neuroprotective signaling pathway to maintain dendrite connectivity
Title: PINK1 regulates mitochondrial trafficking in dendrites of cortical neurons through mitochondrial PKA.

Data suggest that enzyme activation by cAMP involves highly stable conformation of Prkar1a as it binds to Prkaca; glycine residue, G235, appears to function as hinge in B/C helix conserved in Prkar1a; this "Flipback" conformation plays role in cAMP association to A domain of Prkar1a. (Prkar1a = cyclic AMP-dependent protein kinase RIalpha subunit; Prkaca = cyclic AMP-dependent protein kinase catalytic subunit)
Title: Molecular Simulations Reveal an Unresolved Conformation of the Type IA Protein Kinase A Regulatory Subunit and Suggest Its Role in the cAMP Regulatory Mechanism.

Data show that laminin alpha2beta1gamma1 (Lm211) can inhibit neuregulin 1 type III (Nrg1III) by limiting protein kinase A (PKA) activation, which is required to initiate myelination.
Title: Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination.

The following Prkaca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkaca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19784	NM_001277898.1 Latest version!	Mus musculus protein kinase, cAMP dependent, catalytic, alpha (Prkaca), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu19757	NM_008854.5 Latest version!	Mus musculus protein kinase, cAMP dependent, catalytic, alpha (Prkaca), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkaca ( NM_001277898.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264827.1 Mus musculus protein kinase, cAMP dependent, catalytic, alpha (Prkaca), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19784
Clone ID Related Accession (Same CDS sequence)	NM_001277898.1
Accession Version	NM_001277898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF239743.1, CD807077.1, CB248596.1 and BQ031203.1. Transcript Variant: This variant (2, also known as Cs and Calpha2) contains an alternate 5'-most exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct and shorter N-terminus, compared to isoform 1. This variant is testis-specific and the encoded isoform does not contain the myristylation signal found in isoform 1 (PMID: 10841548 and PMID: 10982398). There are no publicly available full-length transcripts for this variant, but it is supported by publication data, including PMID: 10982398. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849390 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 10982398, 10841548, 15340140 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTCCA GCTCCAACGA TGTGAAAGAG TTCCTAGCCA AAGCCAAGGA AGATTTCCTG 
AAAAAATGGG AGACCCCTTC TCAGAATACA GCCCAGTTGG ATCAGTTTGA TAGAATCAAG 
ACCCTTGGCA CCGGCTCCTT TGGGCGAGTG ATGCTGGTGA AGCACAAGGA GAGTGGGAAC 
CACTACGCCA TGAAGATCTT AGACAAGCAG AAGGTGGTGA AGCTAAAGCA GATCGAGCAC 
ACTCTGAATG AGAAGCGCAT CCTGCAGGCC GTCAACTTCC CGTTCCTGGT CAAACTTGAA 
TTCTCCTTCA AGGACAACTC AAACCTGTAC ATGGTCATGG AGTATGTAGC TGGTGGCGAG 
ATGTTCTCCC ACCTACGGCG GATTGGAAGG TTCAGCGAGC CCCATGCCCG TTTCTACGCG 
GCGCAGATCG TCCTGACCTT TGAGTATCTG CACTCCCTGG ACCTCATCTA CCGGGACCTG 
AAGCCCGAGA ATCTTCTCAT CGACCAGCAG GGCTATATTC AGGTGACAGA CTTCGGTTTT 
GCCAAGCGTG TGAAAGGCCG TACTTGGACC TTGTGTGGGA CCCCTGAGTA CTTGGCCCCC 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCTGTGGACT GGTGGGCTCT CGGAGTCCTC 
ATCTACGAGA TGGCTGCTGG TTACCCACCC TTCTTCGCTG ACCAGCCTAT CCAGATCTAT 
GAGAAAATCG TCTCTGGGAA GGTGCGGTTC CCATCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTTCTGCA GGTGGATCTC ACCAAGCGCT TTGGGAACCT CAAGAACGGG 
GTCAATGACA TCAAGAACCA CAAGTGGTTT GCCACGACTG ACTGGATTGC CATCTATCAG 
AGAAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGACAC GAGTAACTTT 
GACGACTATG AGGAGGAAGA GATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
ACTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264827.1
CDS	191..1222
Translation	MASSSNDVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF

Target ORF information:

RefSeq Version	NM_001277898.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, cAMP dependent, catalytic, alpha (Prkaca), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277898.1

ORF Insert Sequence:

ATGGCTTCCA GCTCCAACGA TGTGAAAGAG TTCCTAGCCA AAGCCAAGGA AGATTTCCTG 
AAAAAATGGG AGACCCCTTC TCAGAATACA GCCCAGTTGG ATCAGTTTGA TAGAATCAAG 
ACCCTTGGCA CCGGCTCCTT TGGGCGAGTG ATGCTGGTGA AGCACAAGGA GAGTGGGAAC 
CACTACGCCA TGAAGATCTT AGACAAGCAG AAGGTGGTGA AGCTAAAGCA GATCGAGCAC 
ACTCTGAATG AGAAGCGCAT CCTGCAGGCC GTCAACTTCC CGTTCCTGGT CAAACTTGAA 
TTCTCCTTCA AGGACAACTC AAACCTGTAC ATGGTCATGG AGTATGTAGC TGGTGGCGAG 
ATGTTCTCCC ACCTACGGCG GATTGGAAGG TTCAGCGAGC CCCATGCCCG TTTCTACGCG 
GCGCAGATCG TCCTGACCTT TGAGTATCTG CACTCCCTGG ACCTCATCTA CCGGGACCTG 
AAGCCCGAGA ATCTTCTCAT CGACCAGCAG GGCTATATTC AGGTGACAGA CTTCGGTTTT 
GCCAAGCGTG TGAAAGGCCG TACTTGGACC TTGTGTGGGA CCCCTGAGTA CTTGGCCCCC 
GAGATTATCC TGAGCAAAGG CTACAACAAG GCTGTGGACT GGTGGGCTCT CGGAGTCCTC 
ATCTACGAGA TGGCTGCTGG TTACCCACCC TTCTTCGCTG ACCAGCCTAT CCAGATCTAT 
GAGAAAATCG TCTCTGGGAA GGTGCGGTTC CCATCCCACT TCAGCTCTGA CTTGAAGGAC 
CTGCTGCGGA ACCTTCTGCA GGTGGATCTC ACCAAGCGCT TTGGGAACCT CAAGAACGGG 
GTCAATGACA TCAAGAACCA CAAGTGGTTT GCCACGACTG ACTGGATTGC CATCTATCAG 
AGAAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGACAC GAGTAACTTT 
GACGACTATG AGGAGGAAGA GATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT 
ACTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkaca ( NM_008854.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032880.1 Mus musculus protein kinase, cAMP dependent, catalytic, alpha (Prkaca), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19757
Clone ID Related Accession (Same CDS sequence)	NM_008854.5
Accession Version	NM_008854.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	cAMP-dependent protein kinase catalytic subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC156028.2, BU059097.1, CD807077.1, CB248596.1 and BQ031203.1. On Apr 27, 2013 this sequence version replaced NM_008854.4. Transcript Variant: This variant (1, also known as Calpha1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054834.1, BC003238.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAGTTCCTA 
GCCAAAGCCA AGGAAGATTT CCTGAAAAAA TGGGAGACCC CTTCTCAGAA TACAGCCCAG 
TTGGATCAGT TTGATAGAAT CAAGACCCTT GGCACCGGCT CCTTTGGGCG AGTGATGCTG 
GTGAAGCACA AGGAGAGTGG GAACCACTAC GCCATGAAGA TCTTAGACAA GCAGAAGGTG 
GTGAAGCTAA AGCAGATCGA GCACACTCTG AATGAGAAGC GCATCCTGCA GGCCGTCAAC 
TTCCCGTTCC TGGTCAAACT TGAATTCTCC TTCAAGGACA ACTCAAACCT GTACATGGTC 
ATGGAGTATG TAGCTGGTGG CGAGATGTTC TCCCACCTAC GGCGGATTGG AAGGTTCAGC 
GAGCCCCATG CCCGTTTCTA CGCGGCGCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCC 
CTGGACCTCA TCTACCGGGA CCTGAAGCCC GAGAATCTTC TCATCGACCA GCAGGGCTAT 
ATTCAGGTGA CAGACTTCGG TTTTGCCAAG CGTGTGAAAG GCCGTACTTG GACCTTGTGT 
GGGACCCCTG AGTACTTGGC CCCCGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCTGTG 
GACTGGTGGG CTCTCGGAGT CCTCATCTAC GAGATGGCTG CTGGTTACCC ACCCTTCTTC 
GCTGACCAGC CTATCCAGAT CTATGAGAAA ATCGTCTCTG GGAAGGTGCG GTTCCCATCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTTC TGCAGGTGGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA CGGGGTCAAT GACATCAAGA ACCACAAGTG GTTTGCCACG 
ACTGACTGGA TTGCCATCTA TCAGAGAAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ACACGAGTAA CTTTGACGAC TATGAGGAGG AAGAGATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTACTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032880.1
CDS	210..1265
Translation	MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF

Target ORF information:

RefSeq Version	NM_008854.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, cAMP dependent, catalytic, alpha (Prkaca), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008854.5

ORF Insert Sequence:

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAGTTCCTA 
GCCAAAGCCA AGGAAGATTT CCTGAAAAAA TGGGAGACCC CTTCTCAGAA TACAGCCCAG 
TTGGATCAGT TTGATAGAAT CAAGACCCTT GGCACCGGCT CCTTTGGGCG AGTGATGCTG 
GTGAAGCACA AGGAGAGTGG GAACCACTAC GCCATGAAGA TCTTAGACAA GCAGAAGGTG 
GTGAAGCTAA AGCAGATCGA GCACACTCTG AATGAGAAGC GCATCCTGCA GGCCGTCAAC 
TTCCCGTTCC TGGTCAAACT TGAATTCTCC TTCAAGGACA ACTCAAACCT GTACATGGTC 
ATGGAGTATG TAGCTGGTGG CGAGATGTTC TCCCACCTAC GGCGGATTGG AAGGTTCAGC 
GAGCCCCATG CCCGTTTCTA CGCGGCGCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCC 
CTGGACCTCA TCTACCGGGA CCTGAAGCCC GAGAATCTTC TCATCGACCA GCAGGGCTAT 
ATTCAGGTGA CAGACTTCGG TTTTGCCAAG CGTGTGAAAG GCCGTACTTG GACCTTGTGT 
GGGACCCCTG AGTACTTGGC CCCCGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCTGTG 
GACTGGTGGG CTCTCGGAGT CCTCATCTAC GAGATGGCTG CTGGTTACCC ACCCTTCTTC 
GCTGACCAGC CTATCCAGAT CTATGAGAAA ATCGTCTCTG GGAAGGTGCG GTTCCCATCC 
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTTC TGCAGGTGGA TCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA CGGGGTCAAT GACATCAAGA ACCACAAGTG GTTTGCCACG 
ACTGACTGGA TTGCCATCTA TCAGAGAAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ACACGAGTAA CTTTGACGAC TATGAGGAGG AAGAGATCCG GGTCTCCATC 
AATGAGAAGT GTGGCAAGGA GTTTACTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility.
Proceedings of the National Academy of Sciences of the United States of America101(37)13483-8(2004 Sep)
Nolan MA,Babcock DF,Wennemuth G,Brown W,Burton KA,McKnight GS

PKA: a portrait of protein kinase dynamics.
Biochimica et biophysica acta1697(1-2)259-69(2004 Mar)
Taylor SS,Yang J,Wu J,Haste NM,Radzio-Andzelm E,Anand G

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Prkaca cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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