Slc25a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc25a3
Entrez Gene ID	18674
Full Name	solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3
Synonyms	5730556H19Rik,PTP,Phc
General protein information	Preferred Names solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 Names phosphate carrier protein, mitochondrial phosphate carrier, mitochondrial phosphate transport protein solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 3 solute carrier family 25 member 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_133668.4	NP_598429.1	phosphate carrier protein, mitochondrial precursor
XM_006513371.3	XP_006513434.1	phosphate carrier protein, mitochondrial isoform X1
NM_133668.3	NP_598429.1	phosphate carrier protein, mitochondrial precursor
XM_006513371.4	XP_006513434.1	phosphate carrier protein, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC25A3	XP_005629314.1
Danio rerio (zebrafish)	slc25a3b	NP_998887.1
Caenorhabditis elegans (roundworm)	F01G4.6	NP_001076672.1
Caenorhabditis elegans (roundworm)	T05F1.8	NP_492561.2
Caenorhabditis elegans (roundworm)	C14C10.1	NP_506148.1
	SLC25A3	XP_001083914.2
Bos taurus (cattle)	SLC25A3	NP_777082.1
Arabidopsis thaliana (thale cress)	PHT3;2	NP_190454.1
Oryza sativa (rice)	Os09g0454600	NP_001063353.1
Xenopus tropicalis (tropical clawed frog)	slc25a3	NP_988928.1
Rattus norvegicus (Norway rat)	Slc25a3	NP_620800.2
Danio rerio (zebrafish)	slc25a3a	NP_957009.1
Pan troglodytes (chimpanzee)	SLC25A3	NP_001233352.1
Mus musculus (house mouse)	Slc25a3	NP_598429.1
Gallus gallus (chicken)	SLC25A3	NP_001006236.1
Drosophila melanogaster (fruit fly)	CG9090	NP_611468.1
Homo sapiens (human)	SLC25A3	NP_002626.1
Caenorhabditis elegans (roundworm)	C33F10.12	NP_494870.1

Gene Ontology
Bibliography

Related articles in PubMed

The mammalian phosphate carrier SLC25A3 is a mitochondrial copper transporter required for cytochrome c oxidase biogenesis.
Boulet A, Vest KE, Maynard MK, Gammon MG, Russell AC, Mathews AT, Cole SE, Zhu X, Phillips CB, Kwong JQ, Dodani SC, Leary SC, Cobine PA
The Journal of biological chemistry(2017 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

RBM24 is a major regulator of muscle-specific alternative splicing.
Yang J, Hung LH, Licht T, Kostin S, Looso M, Khrameeva E, Bindereif A, Schneider A, Braun T
Developmental cell31(1)87-99(2014 Oct)

Genetic deletion of the mitochondrial phosphate carrier desensitizes the mitochondrial permeability transition pore and causes cardiomyopathy.
Kwong JQ, Davis J, Baines CP, Sargent MA, Karch J, Wang X, Huang T, Molkentin JD
Cell death and differentiation21(8)1209-17(2014 Aug)

Genetic manipulation of the cardiac mitochondrial phosphate carrier does not affect permeability transition.
Guti?rrez-Aguilar M, Douglas DL, Gibson AK, Domeier TL, Molkentin JD, Baines CP
Journal of molecular and cellular cardiology72316-25(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mitochondrial PiC is not a critical component of the MPT pore.
Title: Genetic manipulation of the cardiac mitochondrial phosphate carrier does not affect permeability transition.

The results indicated that miR-141 can regulate Slc25a3 protein expression in the diabetic heart. Further, diabetes-induced miRNA changes may influence mitochondrial proteomes and functional processes such as mitochondrial ATP production.
Title: miR-141 as a regulator of the mitochondrial phosphate carrier (Slc25a3) in the type 1 diabetic heart.

The following Slc25a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc25a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15008	NM_133668.4 Latest version!	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu44318	XM_006513371.4 Latest version!	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu15008	NM_133668.3	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu44318	XM_006513371.3	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc25a3 ( NM_133668.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598429.1 Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), mRNA.

Price & Availability↑

CloneID	OMu15008
Clone ID Related Accession (Same CDS sequence)	NM_133668.4 , NM_133668.3
Accession Version	NM_133668.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	phosphate carrier protein, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK150641.1, AK077274.1 and BP769168.1. On Jun 23, 2018 this sequence version replaced NM_133668.3. ##Evidence-Data-START## Transcript exon combination :: AK151887.1, AK077274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGTAC AGTTGTGAAT TTGGCTCCAT GAAGTATTAT 
GCACTGTGTG GCTTTGGTGG GGTCTTAAGT TGTGGGCTGA CACACACTGC TGTTGTTCCC 
CTGGACTTAG TAAAGTGCCG CATGCAGGTG GACCCTCAGA AGTACAAAGG CATATTTAAT 
GGATTCTCCA TTACACTGAA AGAAGATGGC GTTCGTGGTT TGGCTAAAGG ATGGGCCCCA 
ACTTTGATTG GCTATTCCAT GCAAGGGCTC TGCAAATTCG GCTTTTATGA AGTCTTCAAA 
GCCTTATATA GCAACATACT TGGTGAGGAA AACACCTACC TGTGGCGCAC ATCACTGTAT 
TTAGCTTCTT CTGCCAGTGC TGAATTCTTC GCTGACATTG CCCTGGCTCC TATGGAAGCT 
GCTAAAGTTC GAATTCAAAC CCAGCCTGGT TATGCCAACA CCTTGAGGGA AGCTGTTCCC 
AAAATGTATA AAGAGGAAGG CTTAAATGCG TTCTACAAGG GCGTTGCTCC TCTGTGGATG 
AGACAGATCC CGTACACCAT GATGAAGTTC GCCTGCTTTG AACGTACTGT TGAAGCTTTG 
TACAAATTTG TGGTTCCTAA GCCCCGAAGT GAATGTACAA AGGCAGAGCA GCTGGTTGTG 
ACATTTGTGG CAGGTTACAT AGCTGGAGTC TTCTGTGCGA TCGTCTCCCA CCCTGCTGAC 
TCTGTGGTCT CTGTGCTGAA TAAAGAGAAA GGCAGCACCG CGTCTCAGGT CCTGCAGAGG 
CTGGGCTTCA GAGGTGTGTG GAAGGGGCTC TTCGCCCGAA TCATCATGAT TGGCACTCTG 
ACTGCACTAC AGTGGTTCAT CTACGACTCT GTGAAGGTCT ACTTCAGGCT CCCTCGCCCT 
CCTCCCCCTG AGATGCCAGA GTCTCTGAAG AAGAAGCTTG GGTTAACTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598429.1
CDS	57..1130
Translation	MFSSVAHLARANPFNAPHLQLVHDGLSGPRSPPAPPRRSRHLAAAAVEEYSCEFGSMKYYALCGFGGVLSCGLTHTAVVPLDLVKCRMQVDPQKYKGIFNGFSITLKEDGVRGLAKGWAPTLIGYSMQGLCKFGFYEVFKALYSNILGEENTYLWRTSLYLASSASAEFFADIALAPMEAAKVRIQTQPGYANTLREAVPKMYKEEGLNAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKFVVPKPRSECTKAEQLVVTFVAGYIAGVFCAIVSHPADSVVSVLNKEKGSTASQVLQRLGFRGVWKGLFARIIMIGTLTALQWFIYDSVKVYFRLPRPPPPEMPESLKKKLGLTE

Target ORF information:

RefSeq Version	NM_133668.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133668.4

ORF Insert Sequence:

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGTAC AGTTGTGAAT TTGGCTCCAT GAAGTATTAT 
GCACTGTGTG GCTTTGGTGG GGTCTTAAGT TGTGGGCTGA CACACACTGC TGTTGTTCCC 
CTGGACTTAG TAAAGTGCCG CATGCAGGTG GACCCTCAGA AGTACAAAGG CATATTTAAT 
GGATTCTCCA TTACACTGAA AGAAGATGGC GTTCGTGGTT TGGCTAAAGG ATGGGCCCCA 
ACTTTGATTG GCTATTCCAT GCAAGGGCTC TGCAAATTCG GCTTTTATGA AGTCTTCAAA 
GCCTTATATA GCAACATACT TGGTGAGGAA AACACCTACC TGTGGCGCAC ATCACTGTAT 
TTAGCTTCTT CTGCCAGTGC TGAATTCTTC GCTGACATTG CCCTGGCTCC TATGGAAGCT 
GCTAAAGTTC GAATTCAAAC CCAGCCTGGT TATGCCAACA CCTTGAGGGA AGCTGTTCCC 
AAAATGTATA AAGAGGAAGG CTTAAATGCG TTCTACAAGG GCGTTGCTCC TCTGTGGATG 
AGACAGATCC CGTACACCAT GATGAAGTTC GCCTGCTTTG AACGTACTGT TGAAGCTTTG 
TACAAATTTG TGGTTCCTAA GCCCCGAAGT GAATGTACAA AGGCAGAGCA GCTGGTTGTG 
ACATTTGTGG CAGGTTACAT AGCTGGAGTC TTCTGTGCGA TCGTCTCCCA CCCTGCTGAC 
TCTGTGGTCT CTGTGCTGAA TAAAGAGAAA GGCAGCACCG CGTCTCAGGT CCTGCAGAGG 
CTGGGCTTCA GAGGTGTGTG GAAGGGGCTC TTCGCCCGAA TCATCATGAT TGGCACTCTG 
ACTGCACTAC AGTGGTTCAT CTACGACTCT GTGAAGGTCT ACTTCAGGCT CCCTCGCCCT 
CCTCCCCCTG AGATGCCAGA GTCTCTGAAG AAGAAGCTTG GGTTAACTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a3 ( NM_133668.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598429.1 Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), mRNA.

Price & Availability↑

CloneID	OMu15008
Clone ID Related Accession (Same CDS sequence)	NM_133668.4 , NM_133668.3
Accession Version	NM_133668.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	phosphate carrier protein, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK077274.1. On Apr 6, 2007 this sequence version replaced NM_133668.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077274.1, AK151887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGTAC AGTTGTGAAT TTGGCTCCAT GAAGTATTAT 
GCACTGTGTG GCTTTGGTGG GGTCTTAAGT TGTGGGCTGA CACACACTGC TGTTGTTCCC 
CTGGACTTAG TAAAGTGCCG CATGCAGGTG GACCCTCAGA AGTACAAAGG CATATTTAAT 
GGATTCTCCA TTACACTGAA AGAAGATGGC GTTCGTGGTT TGGCTAAAGG ATGGGCCCCA 
ACTTTGATTG GCTATTCCAT GCAAGGGCTC TGCAAATTCG GCTTTTATGA AGTCTTCAAA 
GCCTTATATA GCAACATACT TGGTGAGGAA AACACCTACC TGTGGCGCAC ATCACTGTAT 
TTAGCTTCTT CTGCCAGTGC TGAATTCTTC GCTGACATTG CCCTGGCTCC TATGGAAGCT 
GCTAAAGTTC GAATTCAAAC CCAGCCTGGT TATGCCAACA CCTTGAGGGA AGCTGTTCCC 
AAAATGTATA AAGAGGAAGG CTTAAATGCG TTCTACAAGG GCGTTGCTCC TCTGTGGATG 
AGACAGATCC CGTACACCAT GATGAAGTTC GCCTGCTTTG AACGTACTGT TGAAGCTTTG 
TACAAATTTG TGGTTCCTAA GCCCCGAAGT GAATGTACAA AGGCAGAGCA GCTGGTTGTG 
ACATTTGTGG CAGGTTACAT AGCTGGAGTC TTCTGTGCGA TCGTCTCCCA CCCTGCTGAC 
TCTGTGGTCT CTGTGCTGAA TAAAGAGAAA GGCAGCACCG CGTCTCAGGT CCTGCAGAGG 
CTGGGCTTCA GAGGTGTGTG GAAGGGGCTC TTCGCCCGAA TCATCATGAT TGGCACTCTG 
ACTGCACTAC AGTGGTTCAT CTACGACTCT GTGAAGGTCT ACTTCAGGCT CCCTCGCCCT 
CCTCCCCCTG AGATGCCAGA GTCTCTGAAG AAGAAGCTTG GGTTAACTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598429.1
CDS	53..1126
Translation	MFSSVAHLARANPFNAPHLQLVHDGLSGPRSPPAPPRRSRHLAAAAVEEYSCEFGSMKYYALCGFGGVLSCGLTHTAVVPLDLVKCRMQVDPQKYKGIFNGFSITLKEDGVRGLAKGWAPTLIGYSMQGLCKFGFYEVFKALYSNILGEENTYLWRTSLYLASSASAEFFADIALAPMEAAKVRIQTQPGYANTLREAVPKMYKEEGLNAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKFVVPKPRSECTKAEQLVVTFVAGYIAGVFCAIVSHPADSVVSVLNKEKGSTASQVLQRLGFRGVWKGLFARIIMIGTLTALQWFIYDSVKVYFRLPRPPPPEMPESLKKKLGLTE

Target ORF information:

RefSeq Version	NM_133668.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133668.3

ORF Insert Sequence:

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGTAC AGTTGTGAAT TTGGCTCCAT GAAGTATTAT 
GCACTGTGTG GCTTTGGTGG GGTCTTAAGT TGTGGGCTGA CACACACTGC TGTTGTTCCC 
CTGGACTTAG TAAAGTGCCG CATGCAGGTG GACCCTCAGA AGTACAAAGG CATATTTAAT 
GGATTCTCCA TTACACTGAA AGAAGATGGC GTTCGTGGTT TGGCTAAAGG ATGGGCCCCA 
ACTTTGATTG GCTATTCCAT GCAAGGGCTC TGCAAATTCG GCTTTTATGA AGTCTTCAAA 
GCCTTATATA GCAACATACT TGGTGAGGAA AACACCTACC TGTGGCGCAC ATCACTGTAT 
TTAGCTTCTT CTGCCAGTGC TGAATTCTTC GCTGACATTG CCCTGGCTCC TATGGAAGCT 
GCTAAAGTTC GAATTCAAAC CCAGCCTGGT TATGCCAACA CCTTGAGGGA AGCTGTTCCC 
AAAATGTATA AAGAGGAAGG CTTAAATGCG TTCTACAAGG GCGTTGCTCC TCTGTGGATG 
AGACAGATCC CGTACACCAT GATGAAGTTC GCCTGCTTTG AACGTACTGT TGAAGCTTTG 
TACAAATTTG TGGTTCCTAA GCCCCGAAGT GAATGTACAA AGGCAGAGCA GCTGGTTGTG 
ACATTTGTGG CAGGTTACAT AGCTGGAGTC TTCTGTGCGA TCGTCTCCCA CCCTGCTGAC 
TCTGTGGTCT CTGTGCTGAA TAAAGAGAAA GGCAGCACCG CGTCTCAGGT CCTGCAGAGG 
CTGGGCTTCA GAGGTGTGTG GAAGGGGCTC TTCGCCCGAA TCATCATGAT TGGCACTCTG 
ACTGCACTAC AGTGGTTCAT CTACGACTCT GTGAAGGTCT ACTTCAGGCT CCCTCGCCCT 
CCTCCCCCTG AGATGCCAGA GTCTCTGAAG AAGAAGCTTG GGTTAACTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a3 ( XM_006513371.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513434.1 Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44318
Clone ID Related Accession (Same CDS sequence)	XM_006513371.3 , XM_006513371.4
Accession Version	XM_006513371.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphate carrier protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513371.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGCAG TATAGCTGTG AATATGGATC TGGCAGATTC 
TTTCTCCTCT GTGGCCTCGG AGGAATTATT AGCTGTGGCA CAACACATAC AGCATTGGTT 
CCTCTAGATC TCATTAAATG CAGAATGCAG GTGGACCCTC AGAAGTACAA AGGCATATTT 
AATGGATTCT CCATTACACT GAAAGAAGAT GGCGTTCGTG GTTTGGCTAA AGGATGGGCC 
CCAACTTTGA TTGGCTATTC CATGCAAGGG CTCTGCAAAT TCGGCTTTTA TGAAGTCTTC 
AAAGCCTTAT ATAGCAACAT ACTTGGTGAG GAAAACACCT ACCTGTGGCG CACATCACTG 
TATTTAGCTT CTTCTGCCAG TGCTGAATTC TTCGCTGACA TTGCCCTGGC TCCTATGGAA 
GCTGCTAAAG TTCGAATTCA AACCCAGCCT GGTTATGCCA ACACCTTGAG GGAAGCTGTT 
CCCAAAATGT ATAAAGAGGA AGGCTTAAAT GCGTTCTACA AGGGCGTTGC TCCTCTGTGG 
ATGAGACAGA TCCCGTACAC CATGATGAAG TTCGCCTGCT TTGAACGTAC TGTTGAAGCT 
TTGTACAAAT TTGTGGTTCC TAAGCCCCGA AGTGAATGTA CAAAGGCAGA GCAGCTGGTT 
GTGACATTTG TGGCAGGTTA CATAGCTGGA GTCTTCTGTG CGATCGTCTC CCACCCTGCT 
GACTCTGTGG TCTCTGTGCT GAATAAAGAG AAAGGCAGCA CCGCGTCTCA GGTCCTGCAG 
AGGCTGGGCT TCAGAGGTGT GTGGAAGGGG CTCTTCGCCC GAATCATCAT GATTGGCACT 
CTGACTGCAC TACAGTGGTT CATCTACGAC TCTGTGAAGG TCTACTTCAG GCTCCCTCGC 
CCTCCTCCCC CTGAGATGCC AGAGTCTCTG AAGAAGAAGC TTGGGTTAAC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513434.1
CDS	136..1212
Translation	MFSSVAHLARANPFNAPHLQLVHDGLSGPRSPPAPPRRSRHLAAAAVEEQYSCEYGSGRFFLLCGLGGIISCGTTHTALVPLDLIKCRMQVDPQKYKGIFNGFSITLKEDGVRGLAKGWAPTLIGYSMQGLCKFGFYEVFKALYSNILGEENTYLWRTSLYLASSASAEFFADIALAPMEAAKVRIQTQPGYANTLREAVPKMYKEEGLNAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKFVVPKPRSECTKAEQLVVTFVAGYIAGVFCAIVSHPADSVVSVLNKEKGSTASQVLQRLGFRGVWKGLFARIIMIGTLTALQWFIYDSVKVYFRLPRPPPPEMPESLKKKLGLTE

Target ORF information:

RefSeq Version	XM_006513371.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513371.4

ORF Insert Sequence:

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGCAG TATAGCTGTG AATATGGATC TGGCAGATTC 
TTTCTCCTCT GTGGCCTCGG AGGAATTATT AGCTGTGGCA CAACACATAC AGCATTGGTT 
CCTCTAGATC TCATTAAATG CAGAATGCAG GTGGACCCTC AGAAGTACAA AGGCATATTT 
AATGGATTCT CCATTACACT GAAAGAAGAT GGCGTTCGTG GTTTGGCTAA AGGATGGGCC 
CCAACTTTGA TTGGCTATTC CATGCAAGGG CTCTGCAAAT TCGGCTTTTA TGAAGTCTTC 
AAAGCCTTAT ATAGCAACAT ACTTGGTGAG GAAAACACCT ACCTGTGGCG CACATCACTG 
TATTTAGCTT CTTCTGCCAG TGCTGAATTC TTCGCTGACA TTGCCCTGGC TCCTATGGAA 
GCTGCTAAAG TTCGAATTCA AACCCAGCCT GGTTATGCCA ACACCTTGAG GGAAGCTGTT 
CCCAAAATGT ATAAAGAGGA AGGCTTAAAT GCGTTCTACA AGGGCGTTGC TCCTCTGTGG 
ATGAGACAGA TCCCGTACAC CATGATGAAG TTCGCCTGCT TTGAACGTAC TGTTGAAGCT 
TTGTACAAAT TTGTGGTTCC TAAGCCCCGA AGTGAATGTA CAAAGGCAGA GCAGCTGGTT 
GTGACATTTG TGGCAGGTTA CATAGCTGGA GTCTTCTGTG CGATCGTCTC CCACCCTGCT 
GACTCTGTGG TCTCTGTGCT GAATAAAGAG AAAGGCAGCA CCGCGTCTCA GGTCCTGCAG 
AGGCTGGGCT TCAGAGGTGT GTGGAAGGGG CTCTTCGCCC GAATCATCAT GATTGGCACT 
CTGACTGCAC TACAGTGGTT CATCTACGAC TCTGTGAAGG TCTACTTCAG GCTCCCTCGC 
CCTCCTCCCC CTGAGATGCC AGAGTCTCTG AAGAAGAAGC TTGGGTTAAC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a3 ( XM_006513371.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513434.1 Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), transcript variant X1, mRNA.

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CloneID	OMu44318
Clone ID Related Accession (Same CDS sequence)	XM_006513371.3 , XM_006513371.4
Accession Version	XM_006513371.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphate carrier protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513371.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGCAG TATAGCTGTG AATATGGATC TGGCAGATTC 
TTTCTCCTCT GTGGCCTCGG AGGAATTATT AGCTGTGGCA CAACACATAC AGCATTGGTT 
CCTCTAGATC TCATTAAATG CAGAATGCAG GTGGACCCTC AGAAGTACAA AGGCATATTT 
AATGGATTCT CCATTACACT GAAAGAAGAT GGCGTTCGTG GTTTGGCTAA AGGATGGGCC 
CCAACTTTGA TTGGCTATTC CATGCAAGGG CTCTGCAAAT TCGGCTTTTA TGAAGTCTTC 
AAAGCCTTAT ATAGCAACAT ACTTGGTGAG GAAAACACCT ACCTGTGGCG CACATCACTG 
TATTTAGCTT CTTCTGCCAG TGCTGAATTC TTCGCTGACA TTGCCCTGGC TCCTATGGAA 
GCTGCTAAAG TTCGAATTCA AACCCAGCCT GGTTATGCCA ACACCTTGAG GGAAGCTGTT 
CCCAAAATGT ATAAAGAGGA AGGCTTAAAT GCGTTCTACA AGGGCGTTGC TCCTCTGTGG 
ATGAGACAGA TCCCGTACAC CATGATGAAG TTCGCCTGCT TTGAACGTAC TGTTGAAGCT 
TTGTACAAAT TTGTGGTTCC TAAGCCCCGA AGTGAATGTA CAAAGGCAGA GCAGCTGGTT 
GTGACATTTG TGGCAGGTTA CATAGCTGGA GTCTTCTGTG CGATCGTCTC CCACCCTGCT 
GACTCTGTGG TCTCTGTGCT GAATAAAGAG AAAGGCAGCA CCGCGTCTCA GGTCCTGCAG 
AGGCTGGGCT TCAGAGGTGT GTGGAAGGGG CTCTTCGCCC GAATCATCAT GATTGGCACT 
CTGACTGCAC TACAGTGGTT CATCTACGAC TCTGTGAAGG TCTACTTCAG GCTCCCTCGC 
CCTCCTCCCC CTGAGATGCC AGAGTCTCTG AAGAAGAAGC TTGGGTTAAC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513434.1
CDS	91..1167
Translation	MFSSVAHLARANPFNAPHLQLVHDGLSGPRSPPAPPRRSRHLAAAAVEEQYSCEYGSGRFFLLCGLGGIISCGTTHTALVPLDLIKCRMQVDPQKYKGIFNGFSITLKEDGVRGLAKGWAPTLIGYSMQGLCKFGFYEVFKALYSNILGEENTYLWRTSLYLASSASAEFFADIALAPMEAAKVRIQTQPGYANTLREAVPKMYKEEGLNAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKFVVPKPRSECTKAEQLVVTFVAGYIAGVFCAIVSHPADSVVSVLNKEKGSTASQVLQRLGFRGVWKGLFARIIMIGTLTALQWFIYDSVKVYFRLPRPPPPEMPESLKKKLGLTE

Target ORF information:

RefSeq Version	XM_006513371.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 (Slc25a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513371.3

ORF Insert Sequence:

ATGTTCTCGT CCGTAGCGCA CCTGGCGCGG GCGAACCCCT TCAACGCGCC ACACCTGCAG 
CTGGTGCACG ATGGCCTGTC GGGTCCCCGC AGTCCCCCAG CTCCGCCCCG GCGTTCCCGC 
CACCTGGCCG CCGCCGCCGT GGAAGAGCAG TATAGCTGTG AATATGGATC TGGCAGATTC 
TTTCTCCTCT GTGGCCTCGG AGGAATTATT AGCTGTGGCA CAACACATAC AGCATTGGTT 
CCTCTAGATC TCATTAAATG CAGAATGCAG GTGGACCCTC AGAAGTACAA AGGCATATTT 
AATGGATTCT CCATTACACT GAAAGAAGAT GGCGTTCGTG GTTTGGCTAA AGGATGGGCC 
CCAACTTTGA TTGGCTATTC CATGCAAGGG CTCTGCAAAT TCGGCTTTTA TGAAGTCTTC 
AAAGCCTTAT ATAGCAACAT ACTTGGTGAG GAAAACACCT ACCTGTGGCG CACATCACTG 
TATTTAGCTT CTTCTGCCAG TGCTGAATTC TTCGCTGACA TTGCCCTGGC TCCTATGGAA 
GCTGCTAAAG TTCGAATTCA AACCCAGCCT GGTTATGCCA ACACCTTGAG GGAAGCTGTT 
CCCAAAATGT ATAAAGAGGA AGGCTTAAAT GCGTTCTACA AGGGCGTTGC TCCTCTGTGG 
ATGAGACAGA TCCCGTACAC CATGATGAAG TTCGCCTGCT TTGAACGTAC TGTTGAAGCT 
TTGTACAAAT TTGTGGTTCC TAAGCCCCGA AGTGAATGTA CAAAGGCAGA GCAGCTGGTT 
GTGACATTTG TGGCAGGTTA CATAGCTGGA GTCTTCTGTG CGATCGTCTC CCACCCTGCT 
GACTCTGTGG TCTCTGTGCT GAATAAAGAG AAAGGCAGCA CCGCGTCTCA GGTCCTGCAG 
AGGCTGGGCT TCAGAGGTGT GTGGAAGGGG CTCTTCGCCC GAATCATCAT GATTGGCACT 
CTGACTGCAC TACAGTGGTT CATCTACGAC TCTGTGAAGG TCTACTTCAG GCTCCCTCGC 
CCTCCTCCCC CTGAGATGCC AGAGTCTCTG AAGAAGAAGC TTGGGTTAAC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mammalian phosphate carrier SLC25A3 is a mitochondrial copper transporter required for cytochrome c oxidase biogenesis.
The Journal of biological chemistry(2017 Dec)
Boulet A,Vest KE,Maynard MK,Gammon MG,Russell AC,Mathews AT,Cole SE,Zhu X,Phillips CB,Kwong JQ,Dodani SC,Leary SC,Cobine PA

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

RBM24 is a major regulator of muscle-specific alternative splicing.
Developmental cell31(1)87-99(2014 Oct)
Yang J,Hung LH,Licht T,Kostin S,Looso M,Khrameeva E,Bindereif A,Schneider A,Braun T

Genetic deletion of the mitochondrial phosphate carrier desensitizes the mitochondrial permeability transition pore and causes cardiomyopathy.
Cell death and differentiation21(8)1209-17(2014 Aug)
Kwong JQ,Davis J,Baines CP,Sargent MA,Karch J,Wang X,Huang T,Molkentin JD

Genetic manipulation of the cardiac mitochondrial phosphate carrier does not affect permeability transition.
Journal of molecular and cellular cardiology72316-25(2014 Jul)
Guti?rrez-Aguilar M,Douglas DL,Gibson AK,Domeier TL,Molkentin JD,Baines CP

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Slc25a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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