Pgk1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pgk1
Entrez Gene ID	18655
Full Name	phosphoglycerate kinase 1
Synonyms	Pgk-1
General protein information	Preferred Names phosphoglycerate kinase 1 Names phosphoglycerate kinase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X D\|X 47.36 cM
Summary	The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008828.3	NP_032854.2	phosphoglycerate kinase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PGK1	XP_849992.1
Danio rerio (zebrafish)	pgk1	NP_998552.1
Schizosaccharomyces pombe (fission yeast)	pgk1	NP_596730.1
	PGK1	XP_001100787.1
Drosophila melanogaster (fruit fly)	Pgk	NP_001259958.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008802	XP_314928.2
Arabidopsis thaliana (thale cress)	PGK1	NP_187884.1
Mus musculus (house mouse)	Pgk1	NP_032854.2
Rattus norvegicus (Norway rat)	Pgk1	NP_445743.2
Gallus gallus (chicken)	PGK1	NP_990316.1
Saccharomyces cerevisiae (baker's yeast)	PGK1	NP_009938.2
Arabidopsis thaliana (thale cress)	AT1G56190	NP_176015.1
Pan troglodytes (chimpanzee)	PGK1	NP_001129210.1
Caenorhabditis elegans (roundworm)	pgk-1	NP_491245.1
Kluyveromyces lactis	KLLA0A11011g	XP_451479.1
Eremothecium gossypii	AGOS_AEL038C	NP_984823.2
Magnaporthe oryzae (rice blast fungus)	MGG_05063	XP_003712576.1
Neurospora crassa	NCU07914	XP_962430.1
Homo sapiens (human)	PGK1	NP_000282.1
Bos taurus (cattle)	PGK1	NP_001029471.1
Xenopus tropicalis (tropical clawed frog)	pgk1	NP_001016545.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase.
Lay AJ, Jiang XM, Kisker O, Flynn E, Underwood A, Condron R, Hogg PJ
Nature408(6814)869-73(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pgk1 overexpression, or treatment with terazosin (an FDA-approved small molecule that binds and activates Pgk1), rescued motor axon phenotypes in SMA zebrafish. We conclude that global bioenergetics pathways can be therapeutically manipulated to ameliorate SMA motor neuron phenotypes in vivo.
Title: Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.

PGK domain movement and catalysis is regulated by a spring-loaded release mechanism
Title: A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase.

Results indicate that COX-2 suppression by PGK-1 is independent of its catalytic activity.
Title: Nucleotide-binding domain of phosphoglycerate kinase 1 reduces tumor growth by suppressing COX-2 expression.

Together, these data suggest that PGK1 secreted by PCa regulates bone formation at the metastatic site by increasing osteoblastic activity, decreasing osteoclastic function, and expressing an osteoblastic phenotype by PCa cells.
Title: Expression of PGK1 by prostate cancer cells induces bone formation.

Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells
Title: Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells.

The following Pgk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pgk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16385	NM_008828.3 Latest version!	Mus musculus phosphoglycerate kinase 1 (Pgk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pgk1 ( NM_008828.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032854.2 Mus musculus phosphoglycerate kinase 1 (Pgk1), mRNA.

Price & Availability↑

CloneID	OMu16385
Clone ID Related Accession (Same CDS sequence)	NM_008828.3
Accession Version	NM_008828.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	phosphoglycerate kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY292706.1, AK133877.1 and BE986846.1. On Feb 1, 2014 this sequence version replaced NM_008828.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108372.1, SRR1660815.59351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 11130727 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTTT CCAACAAGCT GACTTTGGAC AAGCTGGACG TGAAGGGGAA GCGGGTCGTG 
ATGAGGGTGG ACTTCAACGT TCCTATGAAG AACAACCAGA TAACAAACAA CCAAAGGATC 
AAGGCTGCTG TTCCAAGCAT CAAATTCTGC TTGGACAATG GAGCCAAGTC CGTTGTCCTT 
ATGAGCCACC TGGGCCGGCC TGATGGTGTT CCCATGCCTG ACAAGTACTC CTTAGAGCCA 
GTTGCTGCTG AACTCAAATC TCTGCTGGGC AAGGATGTTC TGTTCTTGAA GGATTGTGTG 
GGCCCAGAAG TCGAGAATGC CTGTGCCAAC CCAGCGGCTG GGACTGTCAT CCTGCTGGAA 
AACCTCCGCT TTCATGTAGA GGAAGAAGGG AAGGGAAAAG ATGCTTCTGG AAACAAGGTT 
AAAGCTGAGC CGGCCAAAAT TGATGCTTTC CGAGCCTCAC TGTCCAAACT AGGAGATGTC 
TATGTCAATG ATGCTTTTGG GACTGCACAC CGAGCCCATA GCTCCATGGT GGGTGTGAAT 
CTGCCACAGA AGGCTGGTGG ATTTTTGATG AAGAAGGAGC TGAACTACTT TGCCAAGGCT 
TTGGAGAGTC CAGAGCGACC CTTCCTGGCT ATCTTGGGAG GCGCTAAAGT TGCAGACAAG 
ATCCAGCTGA TCAATAATAT GCTAGACAAA GTCAATGAGA TGATCATTGG TGGTGGAATG 
GCCTTTACCT TCCTTAAGGT GCTCAACAAC ATGGAGATTG GCACATCTCT GTATGATGAA 
GAAGGAGCCA AGATTGTCAA AGATCTCATG TCCAAAGCTG AGAAAAATGG TGTGAAGATT 
ACCTTGCCTG TTGACTTTGT CACTGCTGAC AAATTTGATG AGAATGCCAA GACTGGCCAA 
GCTACTGTGG CCTCTGGTAT ACCTGCTGGC TGGATGGGCT TGGACTGTGG TACTGAGAGC 
AGCAAGAAAT ATGCCGAGGC TGTGGGTCGA GCTAAGCAGA TTGTTTGGAA TGGTCCTGTT 
GGGGTATTTG AATGGGAAGC CTTTGCCAGG GGAACCAAGT CACTCATGGA TGAGGTGGTG 
AAAGCCACTT CTAGGGGTTG CATCACTATC ATAGGTGGTG GAGACACTGC CACTTGCTGT 
GCCAAATGGA ACACAGAGGA TAAAGTCAGC CATGTGAGCA CTGGGGGCGG TGCCAGTCTA 
GAGCTCCTGG AAGGTAAAGT CCTTCCTGGG GTGGATGCTC TCAGCAATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032854.2
CDS	152..1405
Translation	MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKVKAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLYDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGTESSKKYAEAVGRAKQIVWNGPVGVFEWEAFARGTKSLMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV

Target ORF information:

RefSeq Version	NM_008828.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoglycerate kinase 1 (Pgk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008828.3

ORF Insert Sequence:

ATGTCGCTTT CCAACAAGCT GACTTTGGAC AAGCTGGACG TGAAGGGGAA GCGGGTCGTG 
ATGAGGGTGG ACTTCAACGT TCCTATGAAG AACAACCAGA TAACAAACAA CCAAAGGATC 
AAGGCTGCTG TTCCAAGCAT CAAATTCTGC TTGGACAATG GAGCCAAGTC CGTTGTCCTT 
ATGAGCCACC TGGGCCGGCC TGATGGTGTT CCCATGCCTG ACAAGTACTC CTTAGAGCCA 
GTTGCTGCTG AACTCAAATC TCTGCTGGGC AAGGATGTTC TGTTCTTGAA GGATTGTGTG 
GGCCCAGAAG TCGAGAATGC CTGTGCCAAC CCAGCGGCTG GGACTGTCAT CCTGCTGGAA 
AACCTCCGCT TTCATGTAGA GGAAGAAGGG AAGGGAAAAG ATGCTTCTGG AAACAAGGTT 
AAAGCTGAGC CGGCCAAAAT TGATGCTTTC CGAGCCTCAC TGTCCAAACT AGGAGATGTC 
TATGTCAATG ATGCTTTTGG GACTGCACAC CGAGCCCATA GCTCCATGGT GGGTGTGAAT 
CTGCCACAGA AGGCTGGTGG ATTTTTGATG AAGAAGGAGC TGAACTACTT TGCCAAGGCT 
TTGGAGAGTC CAGAGCGACC CTTCCTGGCT ATCTTGGGAG GCGCTAAAGT TGCAGACAAG 
ATCCAGCTGA TCAATAATAT GCTAGACAAA GTCAATGAGA TGATCATTGG TGGTGGAATG 
GCCTTTACCT TCCTTAAGGT GCTCAACAAC ATGGAGATTG GCACATCTCT GTATGATGAA 
GAAGGAGCCA AGATTGTCAA AGATCTCATG TCCAAAGCTG AGAAAAATGG TGTGAAGATT 
ACCTTGCCTG TTGACTTTGT CACTGCTGAC AAATTTGATG AGAATGCCAA GACTGGCCAA 
GCTACTGTGG CCTCTGGTAT ACCTGCTGGC TGGATGGGCT TGGACTGTGG TACTGAGAGC 
AGCAAGAAAT ATGCCGAGGC TGTGGGTCGA GCTAAGCAGA TTGTTTGGAA TGGTCCTGTT 
GGGGTATTTG AATGGGAAGC CTTTGCCAGG GGAACCAAGT CACTCATGGA TGAGGTGGTG 
AAAGCCACTT CTAGGGGTTG CATCACTATC ATAGGTGGTG GAGACACTGC CACTTGCTGT 
GCCAAATGGA ACACAGAGGA TAAAGTCAGC CATGTGAGCA CTGGGGGCGG TGCCAGTCTA 
GAGCTCCTGG AAGGTAAAGT CCTTCCTGGG GTGGATGCTC TCAGCAATGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase.
Nature408(6814)869-73(2000 Dec)
Lay AJ,Jiang XM,Kisker O,Flynn E,Underwood A,Condron R,Hogg PJ

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Pgk1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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