Pfkm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfkm
Entrez Gene ID	18642
Full Name	phosphofructokinase, muscle
Synonyms	AI131669,ATP-PFK,PFK-A,PFK-M,Pfk-4,Pfk4,Pfka,Pfkx
General protein information	Preferred Names phosphofructokinase, muscle Names ATP-dependent 6-phosphofructokinase, muscle type 6-phosphofructokinase type A 6-phosphofructokinase, muscle type phosphofructo-1-kinase isozyme A phosphofructokinase 1 phosphofructokinase 4 phosphofructokinase M variant 3/4 phosphofructokinase, polypeptide X phosphofructokinase-1 A isozyme phosphofructokinase-M (connecting peptide) phosphohexokinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006520602.2	XP_006520665.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
NM_001163488.1	NP_001156960.1	ATP-dependent 6-phosphofructokinase, muscle type
XM_006520601.3	XP_006520664.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_030248412.1	XP_030104272.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
NM_001357688.1	NP_001344617.1	ATP-dependent 6-phosphofructokinase, muscle type
NM_001163487.1	NP_001156959.1	ATP-dependent 6-phosphofructokinase, muscle type
NM_021514.4	NP_067489.3	ATP-dependent 6-phosphofructokinase, muscle type

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Rewiring of embryonic glucose metabolism via suppression of PFK-1 and aldolase during mouse chorioallantoic branching.
Miyazawa H, Yamaguchi Y, Sugiura Y, Honda K, Kondo K, Matsuda F, Yamamoto T, Suematsu M, Miura M
Development (Cambridge, England)144(1)63-73(2017 01)

Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue.
Duteil D, Tosic M, Lausecker F, Nenseth HZ, M?ller JM, Urban S, Willmann D, Petroll K, Messaddeq N, Arrigoni L, Manke T, Kornfeld JW, Br?ning JC, Zagoriy V, Meret M, Dengjel J, Kanouni T, Sch?le R
Cell reports17(4)1008-1021(2016 10)

Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells.
Zhang F, Qian X, Qin C, Lin Y, Wu H, Chang L, Luo C, Zhu D
Neuroscience bulletin32(3)205-16(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that the reveal PFK-1 as an important regulator of neurogenesis.
Title: Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells.

These results reveal a novel role of NRX in the regulation of PFK1 activity and in the balance between glycolysis and the pentose phosphate pathway.
Title: Nucleoredoxin regulates glucose metabolism via phosphofructokinase 1.

Posttranslational modification of PFK1 enzyme might be the pivotal factor of deregulated glycolytic flux in tumors.
Title: Posttranslational modification of 6-phosphofructo-1-kinase as an important feature of cancer metabolism.

HK1S is tethered in the principal piece region by PFKMS, which in turn is bound tightly to GSTM5 in the fibrous sheath of sperm.
Title: Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5.

In skeletal muscle, the lack of PFK activity blocked glycolysis and resulted in considerable glycogen storage and low ATP content.
Title: Phosphofructo-1-kinase deficiency leads to a severe cardiac and hematological disorder in addition to skeletal muscle glycogenosis.

The following Pfkm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfkm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22441	NM_001163487.1 Latest version!	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22441	NM_021514.4 Latest version!	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22441	NM_001163488.1 Latest version!	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22441	NM_001357688.1 Latest version!	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22441	XM_006520602.2 Latest version!	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu51056	XM_006520601.3 Latest version!	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu22441	XM_030248412.1 Latest version!	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfkm ( NM_001163488.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156960.1 Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu22441
Clone ID Related Accession (Same CDS sequence)	XM_006520602.2 , NM_001163488.1 , XM_030248412.1 , NM_001357688.1 , NM_001163487.1 , NM_021514.4
Accession Version	NM_001163488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113444.9, BY226660.1, AK002711.2 and AA420165.1. Transcript Variant: This variant (3) contains an alternative 5' non-coding exon compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF249894.1, SRR1660819.71335.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156960.1
CDS	86..2428
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001163488.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163488.1

ORF Insert Sequence:

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkm ( NM_001357688.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344617.1 Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu22441
Clone ID Related Accession (Same CDS sequence)	XM_006520602.2 , NM_001163488.1 , XM_030248412.1 , NM_001357688.1 , NM_001163487.1 , NM_021514.4
Accession Version	NM_001357688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113444.9. On Nov 4, 2017 this sequence version replaced XM_006520602.2. Transcript Variant: This variant (4) contains an alternative 5' non-coding exon compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.241115.1, SRR8865137.24039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344617.1
CDS	114..2456
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001357688.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357688.1

ORF Insert Sequence:

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkm ( NM_001163487.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156959.1 Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22441
Clone ID Related Accession (Same CDS sequence)	XM_006520602.2 , NM_001163488.1 , XM_030248412.1 , NM_001357688.1 , NM_001163487.1 , NM_021514.4
Accession Version	NM_001163487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI930496.1, CX218942.1, AK002711.2 and AA420165.1. Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002711.2, AK049773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156959.1
CDS	150..2492
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001163487.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163487.1

ORF Insert Sequence:

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkm ( NM_021514.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067489.3 Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22441
Clone ID Related Accession (Same CDS sequence)	XM_006520602.2 , NM_001163488.1 , XM_030248412.1 , NM_001357688.1 , NM_001163487.1 , NM_021514.4
Accession Version	NM_021514.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY782498.1, AK002711.2 and AA420165.1. On Jul 22, 2009 this sequence version replaced NM_021514.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF249894.1, SRR1660819.71335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067489.3
CDS	103..2445
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	NM_021514.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021514.4

ORF Insert Sequence:

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkm ( XM_006520602.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520665.1 Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X2, mRNA.

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CloneID	OMu22441
Clone ID Related Accession (Same CDS sequence)	XM_006520602.2 , NM_001163488.1 , XM_030248412.1 , NM_001357688.1 , NM_001163487.1 , NM_021514.4
Accession Version	XM_006520602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520665.1
CDS	201..2543
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	XM_006520602.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520602.2

ORF Insert Sequence:

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkm ( XM_006520601.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520664.1 Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51056
Clone ID Related Accession (Same CDS sequence)	XM_006520601.3
Accession Version	XM_006520601.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520601.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTAGAG AGGAGTTTCA GCTGAGATTT TTCATGTGCG TGATTGAGTC TCGCCAAGTA 
GTCAGGACTA CTCAGAGCAC AGCGGGGGAA GCTTCAACCT CCAACATGAC CATACCGGAG 
TCAAAGGCAG ATGACCAGTG GAGACTAGAC GGTATGGATG ATGATCCAAG CACCGTGGGA 
CCTGTATCTA TTCCTGACAC TGAGTGGATC ATGACCCATG AAGAGCATCA TGCAGCCAAA 
ACCCTGGGGA TCGGCAAGGC CATCGCCGTG TTGACCTCTG GTGGAGATGC CCAAGGTATG 
AATGCTGCCG TCAGGGCTGT GGTCCGAGTT GGTATCTTCA CGGGCGCCCG CGTCTTCTTT 
GTCCATGAGG GTTACCAAGG CCTGGTGGAC GGTGGCGAGC ACATCAGGGA GGCCACGTGG 
GAGAGCGTGT CTATGATGCT TCAGCTGGGT GGCACAGTGA TTGGAAGTGC CCGATGCAAG 
GACTTCCGAG AGCGAGAAGG ACGACTCCGG GCCGCCCACA ACCTGGTGAA GCGGGGGATC 
ACCAATCTGT GTGTCATCGG AGGCGATGGC AGCCTCACTG GGGCTGACAC TTTCCGTTCA 
GAGTGGAGCG ACTTGCTGAA TGATCTCCAG AAAGACGGGA AGATCACAGC CGAGGAGGCT 
ACAAAGTCCA GCTACCTGAA CATCGTGGGC CTGGTTGGCT CAATCGACAA TGACTTCTGT 
GGCACTGATA TGACCATTGG TACCGACTCT GCCCTGCACA GGATTGTGGA GATCGTAGAC 
GCCATCACCA CCACGGCTCA GAGCCACCAG AGGACGTTTG TGTTAGAAGT GATGGGCCGC 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTGTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAGTGTC CGCCAGACGA TGACTGGGAA GAACACCTTT GTCGCCGGCT CAGTGAGACA 
AGGACCCGTG GCTCTCGTCT CAACATCATC ATTGTTGCTG AAGGTGCCAT CGACAAGAAC 
GGGAAGCCAA TCACCTCAGA AGACATCAAG AACCTGGTGG TGAAGCGCCT TGGATATGAC 
ACCCGGGTCA CTGTTCTGGG ACATGTACAG CGGGGTGGGA CACCATCAGC CTTTGACCGG 
ATCCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGG GACCCCAGAC 
ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT AACCAGGCTG TGCGTTTGCC CCTCATGGAG 
TGCGTGCAGG TGACCAAAGA CGTGACCAAG GCTATGGATG AGAAGAGATT TGATGAAGCC 
ATTAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TATACAAGCT TCTAGCTCAT 
GTCAGACCCC CAGTCTCTAA GGGTGGGTTG CACACAGTGG CCGTGATGAA TGTGGGGGCC 
CCAGCTGCTG GCATGAATGC CGCTGTTCGC TCTACCGTGA GGATTGGCCT TATCCAGGGC 
AACCGGGTGC TGGTCGTACA TGATGGCTTT GAGGGTCTGG CCAAAGGTCA GATTGAGGAG 
GCTGGCTGGA GCTATGTTGG AGGCTGGACT GGTCAAGGTG GTTCCAAACT TGGTACTAAA 
AGGACTCTCC CCAAGAAGAA CCTGGAACAG ATCAGTGCCA ACATAACCAA GTTTAACATC 
CAGGGCCTTG TCATCATTGG GGGCTTTGAG GCTTACACAG GGGGCTTGGA GCTGATGGAA 
GGCAGGAAGC AGTTTGATGA GCTCTGCATC CCGTTTGTGG TCATTCCGGC CACGGTTTCC 
AATAACGTCC CTGGGTCAGA CTTCAGCATC GGGGCTGACA CAGCACTGAA CACCATCTGC 
ACGACTTGTG ACCGAATCAA GCAGTCTGCA GCAGGCACCA AACGTCGGGT GTTTATCATC 
GAGACTATGG GTGGCTACTG TGGCTACCTG GCCACCATGG CAGGGCTGGC CGCTGGGGCT 
GATGCTGCCT ACATTTTTGA GGAACCCTTC ACCATTCGCG ATCTCCAGGT GAATGTTGAA 
CATCTGGTGC AGAAGATGAA AACAACTGTG AAGAGAGGCC TGGTGCTGAG GAATGAGAAA 
TGCAATGAGA ACTACACTAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTTGATA GCAGGAAGAA CGTGCTTGGC CACATGCAGC AGGGTGGAAG CCCAACTCCC 
TTTGACAGGA ATTTTGCCAC TAAGATGGGT GCTAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAGGAGA GTTACCGTAA TGGACGGATC TTTGCCAACA CCCCTGACTC AGGCTGCGTT 
CTGGGGATGC GTAAGAGGGC CCTGGTCTTT CAGCCAGTAA CTGAGCTGAA GGACCAGACA 
GACTTTGAAC ACCGAATCCC CAAAGAACAG TGGTGGCTGA AGCTGAGGCC AATCCTCAAA 
ATCCTAGCCA AGTACGAGAT TGATCTGGAC ACCTCCGACC ACGCCCACCT GGAGCACATT 
TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520664.1
CDS	292..2844
Translation	MRREEFQLRFFMCVIESRQVVRTTQSTAGEASTSNMTIPESKADDQWRLDGMDDDPSTVGPVSIPDTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	XM_006520601.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520601.3

ORF Insert Sequence:

ATGCGTAGAG AGGAGTTTCA GCTGAGATTT TTCATGTGCG TGATTGAGTC TCGCCAAGTA 
GTCAGGACTA CTCAGAGCAC AGCGGGGGAA GCTTCAACCT CCAACATGAC CATACCGGAG 
TCAAAGGCAG ATGACCAGTG GAGACTAGAC GGTATGGATG ATGATCCAAG CACCGTGGGA 
CCTGTATCTA TTCCTGACAC TGAGTGGATC ATGACCCATG AAGAGCATCA TGCAGCCAAA 
ACCCTGGGGA TCGGCAAGGC CATCGCCGTG TTGACCTCTG GTGGAGATGC CCAAGGTATG 
AATGCTGCCG TCAGGGCTGT GGTCCGAGTT GGTATCTTCA CGGGCGCCCG CGTCTTCTTT 
GTCCATGAGG GTTACCAAGG CCTGGTGGAC GGTGGCGAGC ACATCAGGGA GGCCACGTGG 
GAGAGCGTGT CTATGATGCT TCAGCTGGGT GGCACAGTGA TTGGAAGTGC CCGATGCAAG 
GACTTCCGAG AGCGAGAAGG ACGACTCCGG GCCGCCCACA ACCTGGTGAA GCGGGGGATC 
ACCAATCTGT GTGTCATCGG AGGCGATGGC AGCCTCACTG GGGCTGACAC TTTCCGTTCA 
GAGTGGAGCG ACTTGCTGAA TGATCTCCAG AAAGACGGGA AGATCACAGC CGAGGAGGCT 
ACAAAGTCCA GCTACCTGAA CATCGTGGGC CTGGTTGGCT CAATCGACAA TGACTTCTGT 
GGCACTGATA TGACCATTGG TACCGACTCT GCCCTGCACA GGATTGTGGA GATCGTAGAC 
GCCATCACCA CCACGGCTCA GAGCCACCAG AGGACGTTTG TGTTAGAAGT GATGGGCCGC 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTGTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAGTGTC CGCCAGACGA TGACTGGGAA GAACACCTTT GTCGCCGGCT CAGTGAGACA 
AGGACCCGTG GCTCTCGTCT CAACATCATC ATTGTTGCTG AAGGTGCCAT CGACAAGAAC 
GGGAAGCCAA TCACCTCAGA AGACATCAAG AACCTGGTGG TGAAGCGCCT TGGATATGAC 
ACCCGGGTCA CTGTTCTGGG ACATGTACAG CGGGGTGGGA CACCATCAGC CTTTGACCGG 
ATCCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGG GACCCCAGAC 
ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT AACCAGGCTG TGCGTTTGCC CCTCATGGAG 
TGCGTGCAGG TGACCAAAGA CGTGACCAAG GCTATGGATG AGAAGAGATT TGATGAAGCC 
ATTAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TATACAAGCT TCTAGCTCAT 
GTCAGACCCC CAGTCTCTAA GGGTGGGTTG CACACAGTGG CCGTGATGAA TGTGGGGGCC 
CCAGCTGCTG GCATGAATGC CGCTGTTCGC TCTACCGTGA GGATTGGCCT TATCCAGGGC 
AACCGGGTGC TGGTCGTACA TGATGGCTTT GAGGGTCTGG CCAAAGGTCA GATTGAGGAG 
GCTGGCTGGA GCTATGTTGG AGGCTGGACT GGTCAAGGTG GTTCCAAACT TGGTACTAAA 
AGGACTCTCC CCAAGAAGAA CCTGGAACAG ATCAGTGCCA ACATAACCAA GTTTAACATC 
CAGGGCCTTG TCATCATTGG GGGCTTTGAG GCTTACACAG GGGGCTTGGA GCTGATGGAA 
GGCAGGAAGC AGTTTGATGA GCTCTGCATC CCGTTTGTGG TCATTCCGGC CACGGTTTCC 
AATAACGTCC CTGGGTCAGA CTTCAGCATC GGGGCTGACA CAGCACTGAA CACCATCTGC 
ACGACTTGTG ACCGAATCAA GCAGTCTGCA GCAGGCACCA AACGTCGGGT GTTTATCATC 
GAGACTATGG GTGGCTACTG TGGCTACCTG GCCACCATGG CAGGGCTGGC CGCTGGGGCT 
GATGCTGCCT ACATTTTTGA GGAACCCTTC ACCATTCGCG ATCTCCAGGT GAATGTTGAA 
CATCTGGTGC AGAAGATGAA AACAACTGTG AAGAGAGGCC TGGTGCTGAG GAATGAGAAA 
TGCAATGAGA ACTACACTAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTTGATA GCAGGAAGAA CGTGCTTGGC CACATGCAGC AGGGTGGAAG CCCAACTCCC 
TTTGACAGGA ATTTTGCCAC TAAGATGGGT GCTAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAGGAGA GTTACCGTAA TGGACGGATC TTTGCCAACA CCCCTGACTC AGGCTGCGTT 
CTGGGGATGC GTAAGAGGGC CCTGGTCTTT CAGCCAGTAA CTGAGCTGAA GGACCAGACA 
GACTTTGAAC ACCGAATCCC CAAAGAACAG TGGTGGCTGA AGCTGAGGCC AATCCTCAAA 
ATCCTAGCCA AGTACGAGAT TGATCTGGAC ACCTCCGACC ACGCCCACCT GGAGCACATT 
TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkm ( XM_030248412.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104272.1 Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X2, mRNA.

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CloneID	OMu22441
Clone ID Related Accession (Same CDS sequence)	XM_006520602.2 , NM_001163488.1 , XM_030248412.1 , NM_001357688.1 , NM_001163487.1 , NM_021514.4
Accession Version	XM_030248412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104272.1
CDS	229..2571
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV

Target ORF information:

RefSeq Version	XM_030248412.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248412.1

ORF Insert Sequence:

ATGACCCATG AAGAGCATCA TGCAGCCAAA ACCCTGGGGA TCGGCAAGGC CATCGCCGTG 
TTGACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCCG TCAGGGCTGT GGTCCGAGTT 
GGTATCTTCA CGGGCGCCCG CGTCTTCTTT GTCCATGAGG GTTACCAAGG CCTGGTGGAC 
GGTGGCGAGC ACATCAGGGA GGCCACGTGG GAGAGCGTGT CTATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGATGCAAG GACTTCCGAG AGCGAGAAGG ACGACTCCGG 
GCCGCCCACA ACCTGGTGAA GCGGGGGATC ACCAATCTGT GTGTCATCGG AGGCGATGGC 
AGCCTCACTG GGGCTGACAC TTTCCGTTCA GAGTGGAGCG ACTTGCTGAA TGATCTCCAG 
AAAGACGGGA AGATCACAGC CGAGGAGGCT ACAAAGTCCA GCTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATCGACAA TGACTTCTGT GGCACTGATA TGACCATTGG TACCGACTCT 
GCCCTGCACA GGATTGTGGA GATCGTAGAC GCCATCACCA CCACGGCTCA GAGCCACCAG 
AGGACGTTTG TGTTAGAAGT GATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAGTGTC CGCCAGACGA TGACTGGGAA 
GAACACCTTT GTCGCCGGCT CAGTGAGACA AGGACCCGTG GCTCTCGTCT CAACATCATC 
ATTGTTGCTG AAGGTGCCAT CGACAAGAAC GGGAAGCCAA TCACCTCAGA AGACATCAAG 
AACCTGGTGG TGAAGCGCCT TGGATATGAC ACCCGGGTCA CTGTTCTGGG ACATGTACAG 
CGGGGTGGGA CACCATCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAC ACCCCAGCCT GTGTGGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGTTTGCC CCTCATGGAG TGCGTGCAGG TGACCAAAGA CGTGACCAAG 
GCTATGGATG AGAAGAGATT TGATGAAGCC ATTAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTAGCTCAT GTCAGACCCC CAGTCTCTAA GGGTGGGTTG 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCAGCTGCTG GCATGAATGC CGCTGTTCGC 
TCTACCGTGA GGATTGGCCT TATCCAGGGC AACCGGGTGC TGGTCGTACA TGATGGCTTT 
GAGGGTCTGG CCAAAGGTCA GATTGAGGAG GCTGGCTGGA GCTATGTTGG AGGCTGGACT 
GGTCAAGGTG GTTCCAAACT TGGTACTAAA AGGACTCTCC CCAAGAAGAA CCTGGAACAG 
ATCAGTGCCA ACATAACCAA GTTTAACATC CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCTTGGA GCTGATGGAA GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCGTTTGTGG TCATTCCGGC CACGGTTTCC AATAACGTCC CTGGGTCAGA CTTCAGCATC 
GGGGCTGACA CAGCACTGAA CACCATCTGC ACGACTTGTG ACCGAATCAA GCAGTCTGCA 
GCAGGCACCA AACGTCGGGT GTTTATCATC GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCCACCATGG CAGGGCTGGC CGCTGGGGCT GATGCTGCCT ACATTTTTGA GGAACCCTTC 
ACCATTCGCG ATCTCCAGGT GAATGTTGAA CATCTGGTGC AGAAGATGAA AACAACTGTG 
AAGAGAGGCC TGGTGCTGAG GAATGAGAAA TGCAATGAGA ACTACACTAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTTGATA GCAGGAAGAA CGTGCTTGGC 
CACATGCAGC AGGGTGGAAG CCCAACTCCC TTTGACAGGA ATTTTGCCAC TAAGATGGGT 
GCTAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAGGAGA GTTACCGTAA TGGACGGATC 
TTTGCCAACA CCCCTGACTC AGGCTGCGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAGCCAGTAA CTGAGCTGAA GGACCAGACA GACTTTGAAC ACCGAATCCC CAAAGAACAG 
TGGTGGCTGA AGCTGAGGCC AATCCTCAAA ATCCTAGCCA AGTACGAGAT TGATCTGGAC 
ACCTCCGACC ACGCCCACCT GGAGCACATT TCAAGGAAAC GGTCTGGAGA AGCCGCCGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Rewiring of embryonic glucose metabolism via suppression of PFK-1 and aldolase during mouse chorioallantoic branching.
Development (Cambridge, England)144(1)63-73(2017 01)
Miyazawa H,Yamaguchi Y,Sugiura Y,Honda K,Kondo K,Matsuda F,Yamamoto T,Suematsu M,Miura M

Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue.
Cell reports17(4)1008-1021(2016 10)
Duteil D,Tosic M,Lausecker F,Nenseth HZ,M?ller JM,Urban S,Willmann D,Petroll K,Messaddeq N,Arrigoni L,Manke T,Kornfeld JW,Br?ning JC,Zagoriy V,Meret M,Dengjel J,Kanouni T,Sch?le R

Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells.
Neuroscience bulletin32(3)205-16(2016 Jun)
Zhang F,Qian X,Qin C,Lin Y,Wu H,Chang L,Luo C,Zhu D

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Pfkm cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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