Pfkfb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfkfb2
Entrez Gene ID	18640
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Synonyms	4930568D07Rik
General protein information	Preferred Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 Names 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 6PF-2-K/Fru-2,6-P2ase 2 6PF-2-K/Fru-2,6-P2ase heart-type isozyme PFK-2/FBPase-2 gene B PFK/FBPase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001162415.1	NP_001155887.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 2
NM_008825.4	NP_032851.2	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 1
NM_001162416.1	NP_001155888.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 3
NM_001368843.1	NP_001355772.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pfkfb2	NP_032851.2
Magnaporthe oryzae (rice blast fungus)	MGG_06524	XP_003717027.1
Oryza sativa (rice)	Os03g0294200	NP_001049815.1
Homo sapiens (human)	PFKFB2	NP_006203.2
Danio rerio (zebrafish)	pfkfb2a	NP_957302.1
Kluyveromyces lactis	KLLA0B07513g	XP_451863.1
Pan troglodytes (chimpanzee)	PFKFB2	XP_001165997.2
	PFKFB2	XP_001082732.1
Schizosaccharomyces pombe (fission yeast)	SPAPB17E12.14c	NP_001018229.2
Neurospora crassa	NCU09347	XP_958926.1
Gallus gallus (chicken)	PFKFB2	XP_004935023.1
Eremothecium gossypii	AGOS_AEL106W	NP_984755.2
Canis lupus familiaris (dog)	PFKFB2	XP_005622364.1
Bos taurus (cattle)	PFKFB2	NP_777237.3
Rattus norvegicus (Norway rat)	Pfkfb2	NP_001029136.1
Danio rerio (zebrafish)	LOC564852	XP_001919792.3
Saccharomyces cerevisiae (baker's yeast)	FBP26	NP_012380.1
Xenopus tropicalis (tropical clawed frog)	pfkfb2	NP_001123395.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac Insulin Signaling Regulates Glycolysis Through Phosphofructokinase 2 Content and Activity.
Bockus LB, Matsuzaki S, Vadvalkar SS, Young ZT, Giorgione JR, Newhardt MF, Kinter M, Humphries KM
Journal of the American Heart Association6(12)(2017 Dec)

Mitochondrial dysfunction is an important cause of neurological deficits in an inflammatory model of multiple sclerosis.
Sadeghian M, Mastrolia V, Rezaei Haddad A, Mosley A, Mullali G, Schiza D, Sajic M, Hargreaves I, Heales S, Duchen MR, Smith KJ
Scientific reports633249(2016 Sep)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Sucrose non-fermenting related kinase enzyme is essential for cardiac metabolism.
Cossette SM, Gastonguay AJ, Bao X, Lerch-Gaggl A, Zhong L, Harmann LM, Koceja C, Miao RQ, Vakeel P, Chun C, Li K, Foeckler J, Bordas M, Weiler H, Strande J, Palecek SP, Ramchandran R
Biology open4(1)48-61(2014 Dec)

Glucocorticoid receptor is required for foetal heart maturation.
Rog-Zielinska EA, Thomson A, Kenyon CJ, Brownstein DG, Moran CM, Szumska D, Michailidou Z, Richardson J, Owen E, Watt A, Morrison H, Forrester LM, Bhattacharya S, Holmes MC, Chapman KE
Human molecular genetics22(16)3269-82(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SGK3 is not required for insulin-induced PFK-2 activation and that this effect is likely mediated by PKBalpha.
Title: Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), but not SGK3 (serum- and glucocorticoid-induced protein kinase 3).

mouse islets expressed PFKFB2 at the mRNA level; PFK-2/FBPase-2 protein rather than its product fructose 2,6-P(2) is the over-riding determinant of glucose-induced insulin secretion through regulation of glucokinase activity or subcellular targeting.
Title: A role for PFK-2/FBPase-2, as distinct from fructose 2,6-bisphosphate, in regulation of insulin secretion in pancreatic beta-cells.

Our results demonstrate that in diet-induced obesity, high Fru-2,6-P2 levels in transgenic livers caused changes in hepatic gene expression profiles for key gluconeogenic and lipogenic enzymes.
Title: Overexpression of ubiquitous 6-phosphofructo-2-kinase in the liver of transgenic mice results in weight gain.

The following Pfkfb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfkfb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15632	NM_001162416.1 Latest version!	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$293.30 $419.00
OMu15895	NM_001162415.1 Latest version!	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15934	NM_008825.4 Latest version!	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OMu84629	NM_001368843.1 Latest version!	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfkfb2 ( NM_001162416.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155888.1 Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15632
Clone ID Related Accession (Same CDS sequence)	NM_001162416.1
Accession Version	NM_001162416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC051014.1 and EU616667.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, and the 5' and 3' splice patterns have not been determined. The resulting isoform (3) lacks an internal segment near the C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU616667.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGGT TGCAAAGTGG AAACAATTAC TCTGAATGTG 
GATGCTGTAG ACACACATCG TGACAAGCCA ACTCACAACT TCCCCAAGAG CCAAACCCCT 
GTAAGGATGA GAAGGAACAG CTTTACGCCT CTGTCCAGTT CGAACACAAT AAGGCGTCCA 
AGAAATTACA GTGTTGGGAG CCGACCCCTC AAGCCCCTCA GCCCTCTCCG TGCTCTGGAC 
ATGCAAGAAG GGGCGGACCA GCCGAAGACC CAAGTCAGCA TTCCGGTGGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155888.1
CDS	21..1514
Translation	MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYQSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRDMILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDNYDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVISHQAVMRCLLAYFLDKGAGCKVETITLNVDAVDTHRDKPTHNFPKSQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRALDMQEGADQPKTQVSIPVV

Target ORF information:

RefSeq Version	NM_001162416.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162416.1

ORF Insert Sequence:

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGGT TGCAAAGTGG AAACAATTAC TCTGAATGTG 
GATGCTGTAG ACACACATCG TGACAAGCCA ACTCACAACT TCCCCAAGAG CCAAACCCCT 
GTAAGGATGA GAAGGAACAG CTTTACGCCT CTGTCCAGTT CGAACACAAT AAGGCGTCCA 
AGAAATTACA GTGTTGGGAG CCGACCCCTC AAGCCCCTCA GCCCTCTCCG TGCTCTGGAC 
ATGCAAGAAG GGGCGGACCA GCCGAAGACC CAAGTCAGCA TTCCGGTGGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb2 ( NM_001162415.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155887.1 Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15895
Clone ID Related Accession (Same CDS sequence)	NM_001162415.1
Accession Version	NM_001162415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029659.1, BC051014.1 and AC139231.11. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, and differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029659.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGAT GAGTTACCAT ACTTGAGGTG CCCCCTGCAC 
ATCATCTTCA AACTTACTCC TGTGGCCTAT GGTTGCAAAG TGGAAACAAT TACTCTGAAT 
GTGGATGCTG TAGACACACA TCGTGACAAG CCAACTGAAG TGGAGAATTT GCTGGCTGAG 
CATAGACACC CCTCAATGGC ATCCCTCACT TTGCTCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155887.1
CDS	228..1649
Translation	MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYQSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRDMILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDNYDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVISHQAVMRCLLAYFLDKGADELPYLRCPLHIIFKLTPVAYGCKVETITLNVDAVDTHRDKPTEVENLLAEHRHPSMASLTLLS

Target ORF information:

RefSeq Version	NM_001162415.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162415.1

ORF Insert Sequence:

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGAT GAGTTACCAT ACTTGAGGTG CCCCCTGCAC 
ATCATCTTCA AACTTACTCC TGTGGCCTAT GGTTGCAAAG TGGAAACAAT TACTCTGAAT 
GTGGATGCTG TAGACACACA TCGTGACAAG CCAACTGAAG TGGAGAATTT GCTGGCTGAG 
CATAGACACC CCTCAATGGC ATCCCTCACT TTGCTCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb2 ( NM_008825.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032851.2 Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15934
Clone ID Related Accession (Same CDS sequence)	NM_008825.4
Accession Version	NM_008825.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139231.11. On Jun 23, 2009 this sequence version replaced NM_008825.3. Transcript Variant: This variant (1) represents the longest transcript and isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018418.1, X98847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGAT GAGTTACCAT ACTTGAGGTG CCCCCTGCAC 
ATCATCTTCA AACTTACTCC TGTGGCCTAT GGTTGCAAAG TGGAAACAAT TACTCTGAAT 
GTGGATGCTG TAGACACACA TCGTGACAAG CCAACTCACA ACTTCCCCAA GAGCCAAACC 
CCTGTAAGGA TGAGAAGGAA CAGCTTTACG CCTCTGTCCA GTTCGAACAC AATAAGGCGT 
CCAAGAAATT ACAGTGTTGG GAGCCGACCC CTCAAGCCCC TCAGCCCTCT CCGTGCTCTG 
GACATGCAAG AAGGGGCGGA CCAGCCGAAG ACCCAAGTCA GCATTCCGGT GGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032851.2
CDS	122..1678
Translation	MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYQSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRDMILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDNYDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVISHQAVMRCLLAYFLDKGADELPYLRCPLHIIFKLTPVAYGCKVETITLNVDAVDTHRDKPTHNFPKSQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRALDMQEGADQPKTQVSIPVV

Target ORF information:

RefSeq Version	NM_008825.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008825.4

ORF Insert Sequence:

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGAT GAGTTACCAT ACTTGAGGTG CCCCCTGCAC 
ATCATCTTCA AACTTACTCC TGTGGCCTAT GGTTGCAAAG TGGAAACAAT TACTCTGAAT 
GTGGATGCTG TAGACACACA TCGTGACAAG CCAACTCACA ACTTCCCCAA GAGCCAAACC 
CCTGTAAGGA TGAGAAGGAA CAGCTTTACG CCTCTGTCCA GTTCGAACAC AATAAGGCGT 
CCAAGAAATT ACAGTGTTGG GAGCCGACCC CTCAAGCCCC TCAGCCCTCT CCGTGCTCTG 
GACATGCAAG AAGGGGCGGA CCAGCCGAAG ACCCAAGTCA GCATTCCGGT GGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb2 ( NM_001368843.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355772.1 Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 5, mRNA.

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CloneID	OMu84629
Clone ID Related Accession (Same CDS sequence)	NM_001368843.1
Accession Version	NM_001368843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139231.11. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.871091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGAT GAGTTACCAT ACTTGAGGTG CCCCCTGCAC 
ATCATCTTCA AACTTACTCC TGTGGCCTAT GGTTGCAAAG TGGAAACAAT TACTCTGAAT 
GTGGATGCTG TAGACACACA TCGTGACAAG CCAACTCACA ACTTCCCCAA GAGCCAAACC 
CCTGTAAGGA TGAGAAGGAA CAGCTTTACG CCTCTGTCCA GTTCGAACAC AATAAGGCGT 
CCAAGAAATT ACAGTGTTGG GAGCCGACCC CTCAAGCCCC TCAGCCCTCT CCGTGCTCTG 
GACATGCAAG AAGGGGCGGA CCAGCCGAAG ACCCAAGAAG TGGAGAATTT GCTGGCTGAG 
CATAGACACC CCTCAATGGC ATCCCTCACT TTGCTCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355772.1
CDS	188..1789
Translation	MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYQSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRDMILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDNYDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVISHQAVMRCLLAYFLDKGADELPYLRCPLHIIFKLTPVAYGCKVETITLNVDAVDTHRDKPTHNFPKSQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRALDMQEGADQPKTQEVENLLAEHRHPSMASLTLLS

Target ORF information:

RefSeq Version	NM_001368843.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368843.1

ORF Insert Sequence:

ATGTCTGAGA ATAGTACATT TTCCCCAGAA GACTGCAACA GCAGCTATAA ACCCCACGCC 
TCAAATCTGC GAAGGGCAGG GAAGACATGC TCATGGGCTT CCTATATGAC CAACTCCCCA 
ACACTCATTG TTATGATTGG CTTGCCAGCC CGGGGTAAAA CTTATGTGTC TAAGAAACTA 
ACACGCTACC TCAACTGGAT TGGAGTACCC ACCAAAGTGT TTAATCTTGG GGTATATCGA 
CGTGAAGCAG TCAAGTCCTA TCAGTCTTAT GATTTCTTTC GACATGACAA TGAAGAGGCT 
ATGAAGATCC GCAAACAGTG TGCTCTGGTG GCACTGGAAG ATGTTAAAGC CTATTTTACT 
GAAGAGAGTG GGCAGATTGC GGTGTTTGAT GCCACCAATA CCACTCGGGA GAGGAGGGAC 
ATGATTTTGA ACTTTGCCAA GCAGAATGCC TTCAAGGTAT TTTTTGTAGA ATCTGTCTGT 
GATGATCCTG ATGTCATTGC TGCCAATATT CTGGAGGTAA AAGTGTCGAG CCCTGACTAC 
CCCGAAAGGA ATAGGGAGAA TGTGATGGAG GACTTCCTGA AGAGAATTGA GTGCTACAAG 
GTCACTTACC AGCCCCTTGA CCCAGACAAC TATGATAAGG ATCTTTCTTT CATAAAGGTG 
ATAAATGTAG GCCAGAGATT CCTGGTCAAC AGAGTTCAGG ACTACATCCA GAGTAAGATT 
GTCTACTACC TGATGAATAT CCACGTCCAT CCTCGCACCA TCTACCTTTG CCGGCACGGC 
GAGAGCGAGT TCAACCTTTT GGGGAAGATT GGGGGTGACT CTGGCCTCTC CGTGCGAGGA 
AAGCAGTTTG CTCATGCTCT GAAGAAGTTT CTGGAGGAAC AGGAGATCCA GGACCTTAAA 
GTGTGGACGA GCCAGTTGAA GAGGACAATT CAGACTGCTG AGTCTCTTGG GGTGACCTAT 
GAGCAGTGGA AGATCCTCAA TGAGATTGAT GCTGGCGTGT GTGAGGAGAT GACATATTCA 
GAGATTGAGC AACGGTACCC AGAGGAATTT GCACTCCGAG ATCAAGAGAA GTATCTGTAC 
CGATACCCTG GTGGGGAGTC CTACCAGGAC CTGGTGCAGC GGCTGGAGCC TGTCATCATG 
GAGCTAGAGC GTCAAGGCAA CATCCTCGTT ATCTCTCACC AGGCTGTCAT GCGCTGCCTC 
CTGGCCTACT TCTTGGATAA AGGCGCAGAT GAGTTACCAT ACTTGAGGTG CCCCCTGCAC 
ATCATCTTCA AACTTACTCC TGTGGCCTAT GGTTGCAAAG TGGAAACAAT TACTCTGAAT 
GTGGATGCTG TAGACACACA TCGTGACAAG CCAACTCACA ACTTCCCCAA GAGCCAAACC 
CCTGTAAGGA TGAGAAGGAA CAGCTTTACG CCTCTGTCCA GTTCGAACAC AATAAGGCGT 
CCAAGAAATT ACAGTGTTGG GAGCCGACCC CTCAAGCCCC TCAGCCCTCT CCGTGCTCTG 
GACATGCAAG AAGGGGCGGA CCAGCCGAAG ACCCAAGAAG TGGAGAATTT GCTGGCTGAG 
CATAGACACC CCTCAATGGC ATCCCTCACT TTGCTCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac Insulin Signaling Regulates Glycolysis Through Phosphofructokinase 2 Content and Activity.
Journal of the American Heart Association6(12)(2017 Dec)
Bockus LB,Matsuzaki S,Vadvalkar SS,Young ZT,Giorgione JR,Newhardt MF,Kinter M,Humphries KM

Mitochondrial dysfunction is an important cause of neurological deficits in an inflammatory model of multiple sclerosis.
Scientific reports633249(2016 Sep)
Sadeghian M,Mastrolia V,Rezaei Haddad A,Mosley A,Mullali G,Schiza D,Sajic M,Hargreaves I,Heales S,Duchen MR,Smith KJ

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Sucrose non-fermenting related kinase enzyme is essential for cardiac metabolism.
Biology open4(1)48-61(2014 Dec)
Cossette SM,Gastonguay AJ,Bao X,Lerch-Gaggl A,Zhong L,Harmann LM,Koceja C,Miao RQ,Vakeel P,Chun C,Li K,Foeckler J,Bordas M,Weiler H,Strande J,Palecek SP,Ramchandran R

Glucocorticoid receptor is required for foetal heart maturation.
Human molecular genetics22(16)3269-82(2013 Aug)
Rog-Zielinska EA,Thomson A,Kenyon CJ,Brownstein DG,Moran CM,Szumska D,Michailidou Z,Richardson J,Owen E,Watt A,Morrison H,Forrester LM,Bhattacharya S,Holmes MC,Chapman KE

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Pfkfb2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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