Pdyn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdyn
Entrez Gene ID	18610
Full Name	prodynorphin
Synonyms	Dyn
General protein information	Preferred Names prodynorphin Names proenkephalin-B alpha-neoendorphin beta-neoendorphin beta-neoendorphin-dynorphin big dynorphin dynorphin A(1-13) dynorphin A(1-17) dynorphin A(1-8) leu-enkephalin leumorphin preprodynorphin rimorphin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F1\|2 63.19 cM
Summary	This gene encodes a preproprotein that is proteolytically cleaved to yield a number of active opium-like peptides. These peptides are the endogenous ligands for the Kappa-opioid receptor and similar G-protein-coupled receptors and are thought to function as the body's natural way to control addiction. These peptides have been associated with depression, stress, anxiety, response to pain, and maintenance of homeostasis via circadian rhythms and control of appetite. Mutations in the related human gene have been linked to the neurodegenerative disease spinocerebellar ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286502.1	NP_001273431.1	proenkephalin-B preproprotein
NM_018863.4	NP_061351.2	proenkephalin-B preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDYN	NP_001177821.1
	PDYN	XP_001113720.1
Danio rerio (zebrafish)	pdyn	NP_001001831.1
Danio rerio (zebrafish)	LOC100536328	XP_003201282.1
Canis lupus familiaris (dog)	PDYN	XP_005634935.1
Bos taurus (cattle)	PDYN	NP_776564.1
Mus musculus (house mouse)	Pdyn	NP_001273431.1
Rattus norvegicus (Norway rat)	Pdyn	NP_062247.2
Gallus gallus (chicken)	PDYN	XP_003642511.1
Pan troglodytes (chimpanzee)	PDYN	XP_003953712.1

Gene Ontology
Bibliography

Related articles in PubMed

Prodynorphin-derived peptides are critical modulators of anxiety and regulate neurochemistry and corticosterone.
Wittmann W, Schunk E, Rosskothen I, Gaburro S, Singewald N, Herzog H, Schwarzer C
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology34(3)775-85(2009 Feb)

Big dynorphin, a prodynorphin-derived peptide produces NMDA receptor-mediated effects on memory, anxiolytic-like and locomotor behavior in mice.
Kuzmin A, Madjid N, Terenius L, Ogren SO, Bakalkin G
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology31(9)1928-37(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These pathways are consistent with previously reported behavioral and biochemical phenotypes that typify mice lacking ENT1. Moreover, we validated decreased expression of the SNARE complex protein VAMP1 (synaptobrevin-1) in the dHip as well as decreased expression of pro-dynorphin (PDYN), neuroendocrine convertase (PCSK1), and Leu-Enkephalin (dynorphin-A) in the nucleus accumbens
Title: Label-Free Neuroproteomics of the Hippocampal-Accumbal Circuit Reveals Deficits in Neurotransmitter and Neuropeptide Signaling in Mice Lacking Ethanol-Sensitive Adenosine Transporter.

steroidal environment and energy state negatively regulate kisspeptin (Kiss1), neurokinin B (Tac2), and dynorphin (Pdyn) gene expression.
Title: The interaction of fasting, caloric restriction, and diet-induced obesity with 17?-estradiol on the expression of KNDy neuropeptides and their receptors in the female mouse.

DYN-A(2-17) interacts with Orexin A in the paraventricular hypothalamic nucleus to modulate food intake in mice.
Title: Role of the non-opioid dynorphin peptide des-Tyr-dynorphin (DYN-A(2-17)) in food intake and physical activity, and its interaction with orexin-A.

This study identify that photostimulation of dyn-expressing cells in discrete subregions within the NAcSh drive opposing motivational behavioral states
Title: Distinct Subpopulations of Nucleus Accumbens Dynorphin Neurons Drive Aversion and Reward.

The results of this study indicated that PDYN have a differential role in cue-induced reinstatement of cocaine-seeking behavior.
Title: Effects of genetic deletion of endogenous opioid system components on the reinstatement of cocaine-seeking behavior in mice.

The following Pdyn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdyn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23269	NM_001286502.1 Latest version!	Mus musculus prodynorphin (Pdyn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu23269	NM_018863.4 Latest version!	Mus musculus prodynorphin (Pdyn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdyn ( NM_001286502.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273431.1 Mus musculus prodynorphin (Pdyn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23269
Clone ID Related Accession (Same CDS sequence)	NM_001286502.1 , NM_018863.4
Accession Version	NM_001286502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	proenkephalin-B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY292284.1, AL808137.4, AK133597.1 and BB421226.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.153511.1, BY292284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTGGT CCAGGCTGAT GCTGGCAGCT TGCCTCCTCG TGATGCCCTC TAATGTTATG 
GCGGACTGCC TGTCCCTGTG CTCCCTGTGT GCAGTGAGGA TTCAGGATGG GCCCCGTCCC 
ATCAACCCCC TGATTTGCTC CCTGGAGTGC CAGGACCTGG TGCCGCCCTC AGAGGAGTGG 
GAGACATGCC GGGGCTTCTC ATCTTTTCTC ACCCTGACGG TCTCTGGGCT CCGTGGCAAG 
GATGACTTGG AAGATGAGGT TGCTTTGGAA GAAGGCTACA GTGCACTAGC CAAGCTCTTG 
GAACCCGTCC TGAAGGAGCT GGAGAAAAGC CGACTCCTTA CCAGCGTCCC AGAGGAAAAG 
TTCAGGGGTC TCTCCAGCAG CTTTGGCAAC GGAAAAGAAT CTGAGCTGGC GGGTGCTGAC 
CGGATGAATG ATGAAGCCGC ACAGGCGGGC ACGCTCCATT TTAATGAGGA GGACTTGAGA 
AAACAGGCCA AACGCTATGG CGGCTTTTTG CGCAAATACC CCAAGAGGAG TTCCGAGATG 
GCCCGGGATG AGGACGGGGG CCAGGATGGG GATCAGGTAG GGCATGAGGA CCTGTACAAA 
CGCTATGGGG GCTTCCTGCG GCGCATTCGC CCCAAGCTGA AGTGGGACAA CCAGAAGCGC 
TATGGTGGTT TCCTGCGGCG TCAGTTCAAG GTGGTGACGC GGTCCCAGGA GAACCCCAAT 
ACCTATTCTG AAGATTTAGA TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273431.1
CDS	118..864
Translation	MAWSRLMLAACLLVMPSNVMADCLSLCSLCAVRIQDGPRPINPLICSLECQDLVPPSEEWETCRGFSSFLTLTVSGLRGKDDLEDEVALEEGYSALAKLLEPVLKELEKSRLLTSVPEEKFRGLSSSFGNGKESELAGADRMNDEAAQAGTLHFNEEDLRKQAKRYGGFLRKYPKRSSEMARDEDGGQDGDQVGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQENPNTYSEDLDV

Target ORF information:

RefSeq Version	NM_001286502.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prodynorphin (Pdyn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286502.1

ORF Insert Sequence:

ATGGCGTGGT CCAGGCTGAT GCTGGCAGCT TGCCTCCTCG TGATGCCCTC TAATGTTATG 
GCGGACTGCC TGTCCCTGTG CTCCCTGTGT GCAGTGAGGA TTCAGGATGG GCCCCGTCCC 
ATCAACCCCC TGATTTGCTC CCTGGAGTGC CAGGACCTGG TGCCGCCCTC AGAGGAGTGG 
GAGACATGCC GGGGCTTCTC ATCTTTTCTC ACCCTGACGG TCTCTGGGCT CCGTGGCAAG 
GATGACTTGG AAGATGAGGT TGCTTTGGAA GAAGGCTACA GTGCACTAGC CAAGCTCTTG 
GAACCCGTCC TGAAGGAGCT GGAGAAAAGC CGACTCCTTA CCAGCGTCCC AGAGGAAAAG 
TTCAGGGGTC TCTCCAGCAG CTTTGGCAAC GGAAAAGAAT CTGAGCTGGC GGGTGCTGAC 
CGGATGAATG ATGAAGCCGC ACAGGCGGGC ACGCTCCATT TTAATGAGGA GGACTTGAGA 
AAACAGGCCA AACGCTATGG CGGCTTTTTG CGCAAATACC CCAAGAGGAG TTCCGAGATG 
GCCCGGGATG AGGACGGGGG CCAGGATGGG GATCAGGTAG GGCATGAGGA CCTGTACAAA 
CGCTATGGGG GCTTCCTGCG GCGCATTCGC CCCAAGCTGA AGTGGGACAA CCAGAAGCGC 
TATGGTGGTT TCCTGCGGCG TCAGTTCAAG GTGGTGACGC GGTCCCAGGA GAACCCCAAT 
ACCTATTCTG AAGATTTAGA TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdyn ( NM_018863.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061351.2 Mus musculus prodynorphin (Pdyn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23269
Clone ID Related Accession (Same CDS sequence)	NM_001286502.1 , NM_018863.4
Accession Version	NM_018863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	proenkephalin-B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK133597.1, AL808137.4 and BB421226.1. On Nov 8, 2013 this sequence version replaced NM_018863.3. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133597.1, SRR1660815.138754.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTGGT CCAGGCTGAT GCTGGCAGCT TGCCTCCTCG TGATGCCCTC TAATGTTATG 
GCGGACTGCC TGTCCCTGTG CTCCCTGTGT GCAGTGAGGA TTCAGGATGG GCCCCGTCCC 
ATCAACCCCC TGATTTGCTC CCTGGAGTGC CAGGACCTGG TGCCGCCCTC AGAGGAGTGG 
GAGACATGCC GGGGCTTCTC ATCTTTTCTC ACCCTGACGG TCTCTGGGCT CCGTGGCAAG 
GATGACTTGG AAGATGAGGT TGCTTTGGAA GAAGGCTACA GTGCACTAGC CAAGCTCTTG 
GAACCCGTCC TGAAGGAGCT GGAGAAAAGC CGACTCCTTA CCAGCGTCCC AGAGGAAAAG 
TTCAGGGGTC TCTCCAGCAG CTTTGGCAAC GGAAAAGAAT CTGAGCTGGC GGGTGCTGAC 
CGGATGAATG ATGAAGCCGC ACAGGCGGGC ACGCTCCATT TTAATGAGGA GGACTTGAGA 
AAACAGGCCA AACGCTATGG CGGCTTTTTG CGCAAATACC CCAAGAGGAG TTCCGAGATG 
GCCCGGGATG AGGACGGGGG CCAGGATGGG GATCAGGTAG GGCATGAGGA CCTGTACAAA 
CGCTATGGGG GCTTCCTGCG GCGCATTCGC CCCAAGCTGA AGTGGGACAA CCAGAAGCGC 
TATGGTGGTT TCCTGCGGCG TCAGTTCAAG GTGGTGACGC GGTCCCAGGA GAACCCCAAT 
ACCTATTCTG AAGATTTAGA TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061351.2
CDS	309..1055
Translation	MAWSRLMLAACLLVMPSNVMADCLSLCSLCAVRIQDGPRPINPLICSLECQDLVPPSEEWETCRGFSSFLTLTVSGLRGKDDLEDEVALEEGYSALAKLLEPVLKELEKSRLLTSVPEEKFRGLSSSFGNGKESELAGADRMNDEAAQAGTLHFNEEDLRKQAKRYGGFLRKYPKRSSEMARDEDGGQDGDQVGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQENPNTYSEDLDV

Target ORF information:

RefSeq Version	NM_018863.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus prodynorphin (Pdyn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018863.4

ORF Insert Sequence:

ATGGCGTGGT CCAGGCTGAT GCTGGCAGCT TGCCTCCTCG TGATGCCCTC TAATGTTATG 
GCGGACTGCC TGTCCCTGTG CTCCCTGTGT GCAGTGAGGA TTCAGGATGG GCCCCGTCCC 
ATCAACCCCC TGATTTGCTC CCTGGAGTGC CAGGACCTGG TGCCGCCCTC AGAGGAGTGG 
GAGACATGCC GGGGCTTCTC ATCTTTTCTC ACCCTGACGG TCTCTGGGCT CCGTGGCAAG 
GATGACTTGG AAGATGAGGT TGCTTTGGAA GAAGGCTACA GTGCACTAGC CAAGCTCTTG 
GAACCCGTCC TGAAGGAGCT GGAGAAAAGC CGACTCCTTA CCAGCGTCCC AGAGGAAAAG 
TTCAGGGGTC TCTCCAGCAG CTTTGGCAAC GGAAAAGAAT CTGAGCTGGC GGGTGCTGAC 
CGGATGAATG ATGAAGCCGC ACAGGCGGGC ACGCTCCATT TTAATGAGGA GGACTTGAGA 
AAACAGGCCA AACGCTATGG CGGCTTTTTG CGCAAATACC CCAAGAGGAG TTCCGAGATG 
GCCCGGGATG AGGACGGGGG CCAGGATGGG GATCAGGTAG GGCATGAGGA CCTGTACAAA 
CGCTATGGGG GCTTCCTGCG GCGCATTCGC CCCAAGCTGA AGTGGGACAA CCAGAAGCGC 
TATGGTGGTT TCCTGCGGCG TCAGTTCAAG GTGGTGACGC GGTCCCAGGA GAACCCCAAT 
ACCTATTCTG AAGATTTAGA TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prodynorphin-derived peptides are critical modulators of anxiety and regulate neurochemistry and corticosterone.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology34(3)775-85(2009 Feb)
Wittmann W,Schunk E,Rosskothen I,Gaburro S,Singewald N,Herzog H,Schwarzer C

Big dynorphin, a prodynorphin-derived peptide produces NMDA receptor-mediated effects on memory, anxiolytic-like and locomotor behavior in mice.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology31(9)1928-37(2006 Sep)
Kuzmin A,Madjid N,Terenius L,Ogren SO,Bakalkin G

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Pdyn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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