Pdha1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdha1
Entrez Gene ID	18597
Full Name	pyruvate dehydrogenase E1 alpha 1
Synonyms	Pdha-1
General protein information	Preferred Names pyruvate dehydrogenase E1 alpha 1 Names pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHE1-A type I pyruvate dehydrogenase E1alpha subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F4\|X 73.95 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008810.3	NP_032836.1	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PDHA1	NP_001012562.1
Neurospora crassa	NCU06482	XP_957122.1
Oryza sativa (rice)	Os02g0739600	NP_001048068.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004773	XP_001689086.1
Xenopus tropicalis (tropical clawed frog)	pdha1	NP_001017072.1
Canis lupus familiaris (dog)	PDHA1	XP_003435519.1
Bos taurus (cattle)	PDHA1	NP_001094516.1
Rattus norvegicus (Norway rat)	Pdha1	NP_001004072.2
Danio rerio (zebrafish)	pdha1a	NP_998558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004786	XP_318026.4
Caenorhabditis elegans (roundworm)	pdha-1	NP_871953.1
Saccharomyces cerevisiae (baker's yeast)	PDA1	NP_011105.4
Schizosaccharomyces pombe (fission yeast)	pda1	NP_594892.1
Magnaporthe oryzae (rice blast fungus)	MGG_06371	XP_003717203.1
Oryza sativa (rice)	Os06g0246500	NP_001057281.1
Homo sapiens (human)	PDHA1	NP_001166926.1
	LOC719277	XP_001113273.2
Mus musculus (house mouse)	Pdha1	NP_032836.1
Danio rerio (zebrafish)	pdha1b	NP_001002399.1
Eremothecium gossypii	AGOS_AGR103W	NP_986769.1
Arabidopsis thaliana (thale cress)	IAR4	NP_173828.1
Arabidopsis thaliana (thale cress)	E1 ALPHA	NP_176198.1
Pan troglodytes (chimpanzee)	PDHA1	NP_001104283.1
Kluyveromyces lactis	KLLA0F12001g	XP_455624.1

Gene Ontology
Bibliography

Related articles in PubMed

Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer.
Chen J, Guccini I, Mitri DD, Brina D, Revandkar A, Sarti M, Pasquini E, Alajati A, Pinton S, Losa M, Civenni G, Catapano CV, Sgrignani J, Cavalli A, D'Antuono R, Asara JM, Morandi A, Chiarugi P, Crotti S, Agostini M, Montopoli M, Masgras I, Rasola A, Garcia-Escudero R, Delaleu N, Rinaldi A, Bertoni F, Bono J, Carracedo A, Alimonti A
Nature genetics(2018 Jan)

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson J, Babu M
Cell systems5(6)564-577.e12(2017 Dec)

Characterization of the impact of glutaredoxin-2 (GRX2) deficiency on superoxide/hydrogen peroxide release from cardiac and liver mitochondria.
Chalker J, Gardiner D, Kuksal N, Mailloux RJ
Redox biology15216-227(2017 Dec)

Acetyl-CoA production from pyruvate is not necessary for preservation of myelin.
Della-Flora Nunes G, Mueller L, Silvestri N, Patel MS, Wrabetz L, Feltri ML, Poitelon Y
Glia65(10)1626-1639(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The association of mitochondrial microphthalmia-associated transcription factor (MITF) with pyruvate dehydrogenase (PDH) emerges as an important regulator of mast cell function. Our findings indicate that PDH could arise as a new target for the manipulation of allergic diseases.
Title: Pyruvate dehydrogenase has a major role in mast cell function, and its activity is regulated by mitochondrial microphthalmia transcription factor.

Taken together, our results indicate that the redox sensing and ROS forming properties of Pdh and Ogdh are linked to S-glutathionylation
Title: Protein S-glutathionylation alters superoxide/hydrogen peroxide emission from pyruvate dehydrogenase complex.

skeletal muscle IL-6 contributes to the regulation of PDH at rest and during prolonged exercise.
Title: Lack of Skeletal Muscle IL-6 Affects Pyruvate Dehydrogenase Activity at Rest and during Prolonged Exercise.

The present findings suggest that IL-6 contributes to regulating the PDHa activity and hence carbohydrate oxidation, but the metabolic state of the muscle seems to determine the outcome of this regulation
Title: Effects of IL-6 on pyruvate dehydrogenase regulation in mouse skeletal muscle.

Systemic Pyruvate Dehydrogenase Deficiency is associated with Cerebral Developmental Abnormalities.
Title: Cerebral Developmental Abnormalities in a Mouse with Systemic Pyruvate Dehydrogenase Deficiency.

The following Pdha1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdha1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14472	NM_008810.3 Latest version!	Mus musculus pyruvate dehydrogenase E1 alpha 1 (Pdha1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdha1 ( NM_008810.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032836.1 Mus musculus pyruvate dehydrogenase E1 alpha 1 (Pdha1), mRNA.

Price & Availability↑

CloneID	OMu14472
Clone ID Related Accession (Same CDS sequence)	NM_008810.3
Accession Version	NM_008810.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY783593.1, AK148459.1 and BQ177075.1. On Aug 25, 2015 this sequence version replaced NM_008810.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007142.1, AK145718.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGAAGA TGCTTGCCGC TGTATCCCGC GTGTTGGCAG GCTCTGCGCA GAAGCCGGCA 
AGCCGAGTGC TGGTTGCTTC CCGTAATTTT GCAAATGATG CTACATTTGA GATTAAGAAA 
TGTGACCTTC ATCGGCTAGA AGAGGGCCCC CCAGTCACCA CAGTGCTCAC CAGAGAGGAT 
GGGCTCAAGT ACTACAGGAT GATGCAGACT GTGCGCCGGA TGGAGCTAAA GGCGGATCAG 
CTGTATAAGC AGAAAATCAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCCTGC 
TGCGTGGGCC TGGAGGCTGG CATAAACCCT ACGGACCACC TCATCACTGC CTATCGAGCA 
CATGGCTTCA CCTTCACTCG GGGCCTGCCT GTGCGAGCAA TTCTTGCAGA GCTAACAGGA 
CGAAGAGGAG GTTGTGCTAA AGGGAAAGGC GGGTCAATGC ACATGTACGC CAAGAACTTC 
TATGGAGGCA ACGGCATCGT TGGAGCTCAG GTGCCCCTGG GAGCAGGAAT TGCCCTGGCC 
TGCAAGTACA ATGGAAAAGA TGAGGTCTGT TTGACATTAT ACGGCGATGG TGCTGCTAAT 
CAGGGTCAGA TCTTTGAAGC TTACAATATG GCAGCACTGT GGAAATTACC TTGCATTTTT 
ATCTGTGAGA ACAACCGCTA TGGCATGGGG ACGTCTGTTG AGAGAGCAGC AGCCAGCACG 
GACTACTACA AAAGAGGAGA TTTTATTCCT GGACTCAGGG TAGATGGAAT GGATATCTTG 
TGCGTCCGAG AGGCAACAAA GTTTGCGGCT GCCTATTGCA GGTCTGGTAA GGGGCCCATC 
CTGATGGAGC TCCAGACTTA CCGCTACCAT GGACACAGCA TGAGTGACCC TGGAGTAAGC 
TACCGCACTC GAGAAGAAAT CCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC 
AAGGATAGAA TGGTGAACAG CAATCTTGCA AGTGTTGAAG AATTAAAGGA GATTGATGTG 
GAAGTGAGGA AAGAAATCGA GGATGCTGCC CAGTTTGCCA CGGCTGATCC TGAGCCCCCG 
TTGGAGGAAC TAGGCTATCA CATCTACAGC AGTGATCCTC CCTTTGAAGT GCGTGGTGCC 
AACCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032836.1
CDS	119..1291
Translation	MRKMLAAVSRVLAGSAQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTGRRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

Target ORF information:

RefSeq Version	NM_008810.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate dehydrogenase E1 alpha 1 (Pdha1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008810.3

ORF Insert Sequence:

ATGAGGAAGA TGCTTGCCGC TGTATCCCGC GTGTTGGCAG GCTCTGCGCA GAAGCCGGCA 
AGCCGAGTGC TGGTTGCTTC CCGTAATTTT GCAAATGATG CTACATTTGA GATTAAGAAA 
TGTGACCTTC ATCGGCTAGA AGAGGGCCCC CCAGTCACCA CAGTGCTCAC CAGAGAGGAT 
GGGCTCAAGT ACTACAGGAT GATGCAGACT GTGCGCCGGA TGGAGCTAAA GGCGGATCAG 
CTGTATAAGC AGAAAATCAT TCGTGGTTTC TGTCACTTGT GTGATGGTCA GGAAGCCTGC 
TGCGTGGGCC TGGAGGCTGG CATAAACCCT ACGGACCACC TCATCACTGC CTATCGAGCA 
CATGGCTTCA CCTTCACTCG GGGCCTGCCT GTGCGAGCAA TTCTTGCAGA GCTAACAGGA 
CGAAGAGGAG GTTGTGCTAA AGGGAAAGGC GGGTCAATGC ACATGTACGC CAAGAACTTC 
TATGGAGGCA ACGGCATCGT TGGAGCTCAG GTGCCCCTGG GAGCAGGAAT TGCCCTGGCC 
TGCAAGTACA ATGGAAAAGA TGAGGTCTGT TTGACATTAT ACGGCGATGG TGCTGCTAAT 
CAGGGTCAGA TCTTTGAAGC TTACAATATG GCAGCACTGT GGAAATTACC TTGCATTTTT 
ATCTGTGAGA ACAACCGCTA TGGCATGGGG ACGTCTGTTG AGAGAGCAGC AGCCAGCACG 
GACTACTACA AAAGAGGAGA TTTTATTCCT GGACTCAGGG TAGATGGAAT GGATATCTTG 
TGCGTCCGAG AGGCAACAAA GTTTGCGGCT GCCTATTGCA GGTCTGGTAA GGGGCCCATC 
CTGATGGAGC TCCAGACTTA CCGCTACCAT GGACACAGCA TGAGTGACCC TGGAGTAAGC 
TACCGCACTC GAGAAGAAAT CCAGGAAGTA AGAAGTAAGA GTGACCCTAT TATGCTTCTC 
AAGGATAGAA TGGTGAACAG CAATCTTGCA AGTGTTGAAG AATTAAAGGA GATTGATGTG 
GAAGTGAGGA AAGAAATCGA GGATGCTGCC CAGTTTGCCA CGGCTGATCC TGAGCCCCCG 
TTGGAGGAAC TAGGCTATCA CATCTACAGC AGTGATCCTC CCTTTGAAGT GCGTGGTGCC 
AACCAGTGGA TCAAGTTTAA GTCAGTCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer.
Nature genetics(2018 Jan)
Chen J,Guccini I,Mitri DD,Brina D,Revandkar A,Sarti M,Pasquini E,Alajati A,Pinton S,Losa M,Civenni G,Catapano CV,Sgrignani J,Cavalli A,D'Antuono R,Asara JM,Morandi A,Chiarugi P,Crotti S,Agostini M,Montopoli M,Masgras I,Rasola A,Garcia-Escudero R,Delaleu N,Rinaldi A,Bertoni F,Bono J,Carracedo A,Alimonti A

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Cell systems5(6)564-577.e12(2017 Dec)
Malty RH,Aoki H,Kumar A,Phanse S,Amin S,Zhang Q,Minic Z,Goebels F,Musso G,Wu Z,Abou-Tok H,Meyer M,Deineko V,Kassir S,Sidhu V,Jessulat M,Scott NE,Xiong X,Vlasblom J,Prasad B,Foster LJ,Alberio T,Garavaglia B,Yu H,Bader GD,Nakamura K,Parkinson J,Babu M

Characterization of the impact of glutaredoxin-2 (GRX2) deficiency on superoxide/hydrogen peroxide release from cardiac and liver mitochondria.
Redox biology15216-227(2017 Dec)
Chalker J,Gardiner D,Kuksal N,Mailloux RJ

Acetyl-CoA production from pyruvate is not necessary for preservation of myelin.
Glia65(10)1626-1639(2017 Oct)
Della-Flora Nunes G,Mueller L,Silvestri N,Patel MS,Wrabetz L,Feltri ML,Poitelon Y

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Pdha1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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