Pde8a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde8a
Entrez Gene ID	18584
Full Name	phosphodiesterase 8A
Synonyms	AI551852,Pde8
General protein information	Preferred Names phosphodiesterase 8A Names high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A mmPDE8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_008803.2	NP_032829.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
NM_008803.3	NP_032829.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PDE8A	XP_001163046.1
Homo sapiens (human)	PDE8A	NP_002596.1
Mus musculus (house mouse)	Pde8a	NP_032829.1
Caenorhabditis elegans (roundworm)	pde-6	NP_490787.1
	PDE8A	XP_001086066.1
Rattus norvegicus (Norway rat)	Pde8a	NP_942062.1
Danio rerio (zebrafish)	pde8a	NP_001074096.1
Canis lupus familiaris (dog)	PDE8A	XP_005618438.1
Xenopus tropicalis (tropical clawed frog)	pde8a	XP_002939668.2
Bos taurus (cattle)	PDE8A	NP_001178384.1
Gallus gallus (chicken)	PDE8A	XP_413834.2

Gene Ontology
Bibliography

Related articles in PubMed

PDE8 controls CD4
Basole CP, Nguyen RK, Lamothe K, Vang A, Clark R, Baillie GS, Epstein PM, Brocke S
Cellular signalling4062-72(2017 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Inhibition of type 5 phosphodiesterase counteracts ?2-adrenergic signalling in beating cardiomyocytes.
Isidori AM, Cornacchione M, Barbagallo F, Di Grazia A, Barrios F, Fassina L, Monaco L, Giannetta E, Gianfrilli D, Garofalo S, Zhang X, Chen X, Xiang YK, Lenzi A, Pellegrini M, Naro F
Cardiovascular research106(3)408-20(2015 Jun)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Phosphodiesterase-8A binds to and regulates Raf-1 kinase.
Brown KM, Day JP, Huston E, Zimmermann B, Hampel K, Christian F, Romano D, Terhzaz S, Lee LC, Willis MJ, Morton DB, Beavo JA, Shimizu-Albergine M, Davies SA, Kolch W, Houslay MD, Baillie GS
Proceedings of the National Academy of Sciences of the United States of America110(16)E1533-42(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggest that both PDE8A and PDE8B play essential roles to maintain low cAMP levels, thereby suppressing resting steroidogenesis by keeping CEH/HSL inactive and StAR protein expression low
Title: cAMP-specific phosphodiesterases 8A and 8B, essential regulators of Leydig cell steroidogenesis.

PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during beta-adrenergic receptor (betaAR) activation in ventricular myocytes.
Title: Phosphodiesterase 8A (PDE8A) regulates excitation-contraction coupling in ventricular myocytes.

PDE8 as a novel target for suppression of Teff cell functions, including adhesion to endothelial cells
Title: PDE8 regulates rapid Teff cell adhesion and proliferation independent of ICER.

pharmacological manipulation of PDE8A, alone or in combination with other PDEs present in Leydig cells, may be exploited to modulate testosterone synthesis
Title: Modulation of Leydig cell function by cyclic nucleotide phosphodiesterase 8A.

The following Pde8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02813	NM_008803.3 Latest version!	Mus musculus phosphodiesterase 8A (Pde8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$482.30 $689.00
View Old Accession Versions >>
OMu02813	NM_008803.2	Mus musculus phosphodiesterase 8A (Pde8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$482.30 $689.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde8a ( NM_008803.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032829.1 Mus musculus phosphodiesterase 8A (Pde8a), mRNA.

Price & Availability↑

CloneID	OMu02813
Clone ID Related Accession (Same CDS sequence)	NM_008803.2 , NM_008803.3
Accession Version	NM_008803.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125578.1. On Jun 3, 2007 this sequence version replaced NM_008803.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125578.1, AF067806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGCG CCCCGAGCAT CCACACTTCC GAAAACCGAA CGTTTAGCCA CAGCGACGGC 
GAGGACGAGG ACGTGGACGT GGACGTCCCG GGCCCCGCGC CGAGATCTAT TCAGCGATGG 
TCGACCGCGC CCGGGCTCGT GGAGCCTCAG CCTCGGGATA ACGGCGCCTC GAAGGTATCA 
GTAGCTGATG TGCAATTTGG CCCGATGAGA TTTCATCAAG ACCAACTTCA GGTCCTGTTA 
GTGTTTACCA AAGAAGATAG CCAGTGTAAC GGATTCCACA GGGCATGTGA GAAAGCTGGG 
TTTAAGTGCA CAGTCACCAA GGAGGTGCAG ACTGTCCTTA CCTGTTTCCA GGACAAACTC 
CATGACATCA TCATCATAGA CCACAGATAT CCCCGACAGA TGGATGCAGA GACATTGTGC 
AGGTCTATCA GATCATCAAA ATTCTCAGAA AACACAGTTA TTGTTGGCGT AGTACGCAGG 
GTGGACAAAG AAGAATCATC TTTAATGCCC TTCCTTGCTG CTGGCTTCAC ACGGAGGTTT 
ATAGAGAACC CCAACGTCAT GGCCTGTTAT AATGAACTGC TCCAGTTGGC ATGTGGAGAG 
GTGCGATCAC AGCTGAAACT CAGGGCTTGT AACTCAGTAT TCACTGCATT AGAGAAAAGT 
CAAGAAGCAA TTGAAATTAC AAGTGAAGAC CACATTATAC AGTATGCAAA CCCTGCATTT 
GAATCAACAA TGGGTTATCA GTCAGGAGAA TTAATAGGGA AGGAGTTAGC ACAAGTGCCC 
ATAAATGAAA AGAAAGGTGA TTTGCTCGAT GCCATAAATT CGTGTGTCAC AGTAGACAAG 
GAATGGCAAG GAGTTTACCA TACCCAAAAG AAAAATGGAG ATAACATACA GCAAAATGTG 
AAGATAATAC CTGTCATTGG GCAGGGAGGA AAAATCAGAC ACTATGTGTC CATTATCAGA 
GTGTGCAATG GCAACAATAA GGTTGAGACC ACAACTGAGT GTGTTCAGAC TGACAGTCAA 
ACAGATAACC AGGCAGGCAA ACATAAGGAC AGGAGGAAAC ATTCGATGGA CGCCAAAGCG 
GTTTCCTCCA GAACAAGTGA CGTTTCCAGT CAGAGAAGAC ATTCCTCTTT GGCCCGGATA 
CATTCTATGA TGATTGAGGC ACCCATCACC AAGGTAATCA ATATTATCAA TGCTGCCCAG 
GAAAACAGTC CTGTGCCTGT GACGGAAGCC TTGAACCGAG TGCTGGATAT TCTGAGAACC 
ACTGAGCTGT ACTCGCCACA GTTTAATGCT CAGGATGATC CCCATGCCAC AGACCTCGTT 
GGGGGCTTAA TGTCTGATGG CTTACGAAGG TTTTCAGGAA ATGAGTATAT TCTTGCAACA 
AAAAACCTCC CACCACTTTC AAACAATTTG GCCACCCCCG TCTCCCTTCA TGATGTCCCA 
CCACGGATTG CCCTGGCCAT AGAAAATGAA GAACAGTGGG ACTTTGACAT TTTTGAGCTG 
GAAGTAGCAA CCCAAAATAG GCCTTTGATT TATCTAGGTC TGAAAACGTT TGCGCGTTTT 
GGAATGTGTG AGTTCTTACA GTGCTCTGAG ACAACGCTGA GATCTTGGTT TCAGATGATC 
GAATCCAACT ACCACTCTTC TAACCCCTAC CACAATTCCA CACACGCTGC GGATGTGCTT 
CACGCCACGG CGTATTTCCT TTCCAGAGAC AAAATAAAGG AAACTTTAGA TCGCATTGAC 
GAGGTTGCTG CCCTCATTGC GGCCACCGTC CATGACGTGG ATCACCCTGG GAGAACCAAC 
TCCTTCCTGT GCAACGCGGG AAACCAGCTG GCTGTGCTCT ACAACGACAC CGCTGTGCTG 
GAGAGCCACC ACGTGGCACT GGCCTTCCAG CTGACGCTTG AAAATGACCA GTGCAATATC 
TTTAAGCAAA TGGAGAGGAA TGACTACCGG ACACTACGCC AGAGCATTAT TGACATGGTC 
CTAGCCACAG AAATGACGAA GCACTTTGAA CATGTCAACA AATTTATCAA CAGCATCAAC 
AAGCCTTTGA CAGCCCAGGA GAGTGAGGAA CCTGACAGAA GTCTGGAAGA CATAAAAGCT 
ATGCTCAAGA CGCCAGAGAG CCGGGCTCTC ATCAAAAGAA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC GCTGGAGCAC TGCATCGAGT GGGCTGCGCG CATCTCAGAG 
GAGTATTTCT CTCAGACTGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCCGTG 
TTTGACAGAA ACACCTGCAG CATTCCCAAG TCTCAGATCT CCTTCATCGA TTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTC GTTGACCTGC CTAACCTTAT GCAGCACCTA 
GATGACAACT TCAGGTACTG GAAAGGACTG GATGAGAAGA AGCTTCGAAG CCTCCGCCCC 
CCTCCAGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032829.1
CDS	112..2583
Translation	MGCAPSIHTSENRTFSHSDGEDEDVDVDVPGPAPRSIQRWSTAPGLVEPQPRDNGASKVSVADVQFGPMRFHQDQLQVLLVFTKEDSQCNGFHRACEKAGFKCTVTKEVQTVLTCFQDKLHDIIIIDHRYPRQMDAETLCRSIRSSKFSENTVIVGVVRRVDKEESSLMPFLAAGFTRRFIENPNVMACYNELLQLACGEVRSQLKLRACNSVFTALEKSQEAIEITSEDHIIQYANPAFESTMGYQSGELIGKELAQVPINEKKGDLLDAINSCVTVDKEWQGVYHTQKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKVETTTECVQTDSQTDNQAGKHKDRRKHSMDAKAVSSRTSDVSSQRRHSSLARIHSMMIEAPITKVINIINAAQENSPVPVTEALNRVLDILRTTELYSPQFNAQDDPHATDLVGGLMSDGLRRFSGNEYILATKNLPPLSNNLATPVSLHDVPPRIALAIENEEQWDFDIFELEVATQNRPLIYLGLKTFARFGMCEFLQCSETTLRSWFQMIESNYHSSNPYHNSTHAADVLHATAYFLSRDKIKETLDRIDEVAALIAATVHDVDHPGRTNSFLCNAGNQLAVLYNDTAVLESHHVALAFQLTLENDQCNIFKQMERNDYRTLRQSIIDMVLATEMTKHFEHVNKFINSINKPLTAQESEEPDRSLEDIKAMLKTPESRALIKRMMIKCADVSNPCRPLEHCIEWAARISEEYFSQTDEEKQLDLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPNLMQHLDDNFRYWKGLDEKKLRSLRPPPE

Target ORF information:

RefSeq Version	NM_008803.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 8A (Pde8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008803.2

ORF Insert Sequence:

ATGGGCTGCG CCCCGAGCAT CCACACTTCC GAAAACCGAA CGTTTAGCCA CAGCGACGGC 
GAGGACGAGG ACGTGGACGT GGACGTCCCG GGCCCCGCGC CGAGATCTAT TCAGCGATGG 
TCGACCGCGC CCGGGCTCGT GGAGCCTCAG CCTCGGGATA ACGGCGCCTC GAAGGTATCA 
GTAGCTGATG TGCAATTTGG CCCGATGAGA TTTCATCAAG ACCAACTTCA GGTCCTGTTA 
GTGTTTACCA AAGAAGATAG CCAGTGTAAC GGATTCCACA GGGCATGTGA GAAAGCTGGG 
TTTAAGTGCA CAGTCACCAA GGAGGTGCAG ACTGTCCTTA CCTGTTTCCA GGACAAACTC 
CATGACATCA TCATCATAGA CCACAGATAT CCCCGACAGA TGGATGCAGA GACATTGTGC 
AGGTCTATCA GATCATCAAA ATTCTCAGAA AACACAGTTA TTGTTGGCGT AGTACGCAGG 
GTGGACAAAG AAGAATCATC TTTAATGCCC TTCCTTGCTG CTGGCTTCAC ACGGAGGTTT 
ATAGAGAACC CCAACGTCAT GGCCTGTTAT AATGAACTGC TCCAGTTGGC ATGTGGAGAG 
GTGCGATCAC AGCTGAAACT CAGGGCTTGT AACTCAGTAT TCACTGCATT AGAGAAAAGT 
CAAGAAGCAA TTGAAATTAC AAGTGAAGAC CACATTATAC AGTATGCAAA CCCTGCATTT 
GAATCAACAA TGGGTTATCA GTCAGGAGAA TTAATAGGGA AGGAGTTAGC ACAAGTGCCC 
ATAAATGAAA AGAAAGGTGA TTTGCTCGAT GCCATAAATT CGTGTGTCAC AGTAGACAAG 
GAATGGCAAG GAGTTTACCA TACCCAAAAG AAAAATGGAG ATAACATACA GCAAAATGTG 
AAGATAATAC CTGTCATTGG GCAGGGAGGA AAAATCAGAC ACTATGTGTC CATTATCAGA 
GTGTGCAATG GCAACAATAA GGTTGAGACC ACAACTGAGT GTGTTCAGAC TGACAGTCAA 
ACAGATAACC AGGCAGGCAA ACATAAGGAC AGGAGGAAAC ATTCGATGGA CGCCAAAGCG 
GTTTCCTCCA GAACAAGTGA CGTTTCCAGT CAGAGAAGAC ATTCCTCTTT GGCCCGGATA 
CATTCTATGA TGATTGAGGC ACCCATCACC AAGGTAATCA ATATTATCAA TGCTGCCCAG 
GAAAACAGTC CTGTGCCTGT GACGGAAGCC TTGAACCGAG TGCTGGATAT TCTGAGAACC 
ACTGAGCTGT ACTCGCCACA GTTTAATGCT CAGGATGATC CCCATGCCAC AGACCTCGTT 
GGGGGCTTAA TGTCTGATGG CTTACGAAGG TTTTCAGGAA ATGAGTATAT TCTTGCAACA 
AAAAACCTCC CACCACTTTC AAACAATTTG GCCACCCCCG TCTCCCTTCA TGATGTCCCA 
CCACGGATTG CCCTGGCCAT AGAAAATGAA GAACAGTGGG ACTTTGACAT TTTTGAGCTG 
GAAGTAGCAA CCCAAAATAG GCCTTTGATT TATCTAGGTC TGAAAACGTT TGCGCGTTTT 
GGAATGTGTG AGTTCTTACA GTGCTCTGAG ACAACGCTGA GATCTTGGTT TCAGATGATC 
GAATCCAACT ACCACTCTTC TAACCCCTAC CACAATTCCA CACACGCTGC GGATGTGCTT 
CACGCCACGG CGTATTTCCT TTCCAGAGAC AAAATAAAGG AAACTTTAGA TCGCATTGAC 
GAGGTTGCTG CCCTCATTGC GGCCACCGTC CATGACGTGG ATCACCCTGG GAGAACCAAC 
TCCTTCCTGT GCAACGCGGG AAACCAGCTG GCTGTGCTCT ACAACGACAC CGCTGTGCTG 
GAGAGCCACC ACGTGGCACT GGCCTTCCAG CTGACGCTTG AAAATGACCA GTGCAATATC 
TTTAAGCAAA TGGAGAGGAA TGACTACCGG ACACTACGCC AGAGCATTAT TGACATGGTC 
CTAGCCACAG AAATGACGAA GCACTTTGAA CATGTCAACA AATTTATCAA CAGCATCAAC 
AAGCCTTTGA CAGCCCAGGA GAGTGAGGAA CCTGACAGAA GTCTGGAAGA CATAAAAGCT 
ATGCTCAAGA CGCCAGAGAG CCGGGCTCTC ATCAAAAGAA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC GCTGGAGCAC TGCATCGAGT GGGCTGCGCG CATCTCAGAG 
GAGTATTTCT CTCAGACTGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCCGTG 
TTTGACAGAA ACACCTGCAG CATTCCCAAG TCTCAGATCT CCTTCATCGA TTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTC GTTGACCTGC CTAACCTTAT GCAGCACCTA 
GATGACAACT TCAGGTACTG GAAAGGACTG GATGAGAAGA AGCTTCGAAG CCTCCGCCCC 
CCTCCAGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde8a ( NM_008803.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032829.1 Mus musculus phosphodiesterase 8A (Pde8a), mRNA.

Price & Availability↑

CloneID	OMu02813
Clone ID Related Accession (Same CDS sequence)	NM_008803.2 , NM_008803.3
Accession Version	NM_008803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CO808457.1, AK148238.1, BC125578.1, AF067806.1 and AV272936.1. On Jun 5, 2018 this sequence version replaced NM_008803.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148238.1, AF067806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCG CCCCGAGCAT CCACACTTCC GAAAACCGAA CGTTTAGCCA CAGCGACGGC 
GAGGACGAGG ACGTGGACGT GGACGTCCCG GGCCCCGCGC CGAGATCTAT TCAGCGATGG 
TCGACCGCGC CCGGGCTCGT GGAGCCTCAG CCTCGGGATA ACGGCGCCTC GAAGGTATCA 
GTAGCTGATG TGCAATTTGG CCCGATGAGA TTTCATCAAG ACCAACTTCA GGTCCTGTTA 
GTGTTTACCA AAGAAGATAG CCAGTGTAAC GGATTCCACA GGGCATGTGA GAAAGCTGGG 
TTTAAGTGCA CAGTCACCAA GGAGGTGCAG ACTGTCCTTA CCTGTTTCCA GGACAAACTC 
CATGACATCA TCATCATAGA CCACAGATAT CCCCGACAGA TGGATGCAGA GACATTGTGC 
AGGTCTATCA GATCATCAAA ATTCTCAGAA AACACAGTTA TTGTTGGCGT AGTACGCAGG 
GTGGACAAAG AAGAATCATC TTTAATGCCC TTCCTTGCTG CTGGCTTCAC ACGGAGGTTT 
ATAGAGAACC CCAACGTCAT GGCCTGTTAT AATGAACTGC TCCAGTTGGC ATGTGGAGAG 
GTGCGATCAC AGCTGAAACT CAGGGCTTGT AACTCAGTAT TCACTGCATT AGAGAAAAGT 
CAAGAAGCAA TTGAAATTAC AAGTGAAGAC CACATTATAC AGTATGCAAA CCCTGCATTT 
GAATCAACAA TGGGTTATCA GTCAGGAGAA TTAATAGGGA AGGAGTTAGC ACAAGTGCCC 
ATAAATGAAA AGAAAGGTGA TTTGCTCGAT GCCATAAATT CGTGTGTCAC AGTAGACAAG 
GAATGGCAAG GAGTTTACCA TACCCAAAAG AAAAATGGAG ATAACATACA GCAAAATGTG 
AAGATAATAC CTGTCATTGG GCAGGGAGGA AAAATCAGAC ACTATGTGTC CATTATCAGA 
GTGTGCAATG GCAACAATAA GGTTGAGACC ACAACTGAGT GTGTTCAGAC TGACAGTCAA 
ACAGATAACC AGGCAGGCAA ACATAAGGAC AGGAGGAAAC ATTCGATGGA CGCCAAAGCG 
GTTTCCTCCA GAACAAGTGA CGTTTCCAGT CAGAGAAGAC ATTCCTCTTT GGCCCGGATA 
CATTCTATGA TGATTGAGGC ACCCATCACC AAGGTAATCA ATATTATCAA TGCTGCCCAG 
GAAAACAGTC CTGTGCCTGT GACGGAAGCC TTGAACCGAG TGCTGGATAT TCTGAGAACC 
ACTGAGCTGT ACTCGCCACA GTTTAATGCT CAGGATGATC CCCATGCCAC AGACCTCGTT 
GGGGGCTTAA TGTCTGATGG CTTACGAAGG TTTTCAGGAA ATGAGTATAT TCTTGCAACA 
AAAAACCTCC CACCACTTTC AAACAATTTG GCCACCCCCG TCTCCCTTCA TGATGTCCCA 
CCACGGATTG CCCTGGCCAT AGAAAATGAA GAACAGTGGG ACTTTGACAT TTTTGAGCTG 
GAAGTAGCAA CCCAAAATAG GCCTTTGATT TATCTAGGTC TGAAAACGTT TGCGCGTTTT 
GGAATGTGTG AGTTCTTACA GTGCTCTGAG ACAACGCTGA GATCTTGGTT TCAGATGATC 
GAATCCAACT ACCACTCTTC TAACCCCTAC CACAATTCCA CACACGCTGC GGATGTGCTT 
CACGCCACGG CGTATTTCCT TTCCAGAGAC AAAATAAAGG AAACTTTAGA TCGCATTGAC 
GAGGTTGCTG CCCTCATTGC GGCCACCGTC CATGACGTGG ATCACCCTGG GAGAACCAAC 
TCCTTCCTGT GCAACGCGGG AAACCAGCTG GCTGTGCTCT ACAACGACAC CGCTGTGCTG 
GAGAGCCACC ACGTGGCACT GGCCTTCCAG CTGACGCTTG AAAATGACCA GTGCAATATC 
TTTAAGCAAA TGGAGAGGAA TGACTACCGG ACACTACGCC AGAGCATTAT TGACATGGTC 
CTAGCCACAG AAATGACGAA GCACTTTGAA CATGTCAACA AATTTATCAA CAGCATCAAC 
AAGCCTTTGA CAGCCCAGGA GAGTGAGGAA CCTGACAGAA GTCTGGAAGA CATAAAAGCT 
ATGCTCAAGA CGCCAGAGAG CCGGGCTCTC ATCAAAAGAA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC GCTGGAGCAC TGCATCGAGT GGGCTGCGCG CATCTCAGAG 
GAGTATTTCT CTCAGACTGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCCGTG 
TTTGACAGAA ACACCTGCAG CATTCCCAAG TCTCAGATCT CCTTCATCGA TTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTC GTTGACCTGC CTAACCTTAT GCAGCACCTA 
GATGACAACT TCAGGTACTG GAAAGGACTG GATGAGAAGA AGCTTCGAAG CCTCCGCCCC 
CCTCCAGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032829.1
CDS	651..3122
Translation	MGCAPSIHTSENRTFSHSDGEDEDVDVDVPGPAPRSIQRWSTAPGLVEPQPRDNGASKVSVADVQFGPMRFHQDQLQVLLVFTKEDSQCNGFHRACEKAGFKCTVTKEVQTVLTCFQDKLHDIIIIDHRYPRQMDAETLCRSIRSSKFSENTVIVGVVRRVDKEESSLMPFLAAGFTRRFIENPNVMACYNELLQLACGEVRSQLKLRACNSVFTALEKSQEAIEITSEDHIIQYANPAFESTMGYQSGELIGKELAQVPINEKKGDLLDAINSCVTVDKEWQGVYHTQKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKVETTTECVQTDSQTDNQAGKHKDRRKHSMDAKAVSSRTSDVSSQRRHSSLARIHSMMIEAPITKVINIINAAQENSPVPVTEALNRVLDILRTTELYSPQFNAQDDPHATDLVGGLMSDGLRRFSGNEYILATKNLPPLSNNLATPVSLHDVPPRIALAIENEEQWDFDIFELEVATQNRPLIYLGLKTFARFGMCEFLQCSETTLRSWFQMIESNYHSSNPYHNSTHAADVLHATAYFLSRDKIKETLDRIDEVAALIAATVHDVDHPGRTNSFLCNAGNQLAVLYNDTAVLESHHVALAFQLTLENDQCNIFKQMERNDYRTLRQSIIDMVLATEMTKHFEHVNKFINSINKPLTAQESEEPDRSLEDIKAMLKTPESRALIKRMMIKCADVSNPCRPLEHCIEWAARISEEYFSQTDEEKQLDLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPNLMQHLDDNFRYWKGLDEKKLRSLRPPPE

Target ORF information:

RefSeq Version	NM_008803.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 8A (Pde8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008803.3

ORF Insert Sequence:

ATGGGCTGCG CCCCGAGCAT CCACACTTCC GAAAACCGAA CGTTTAGCCA CAGCGACGGC 
GAGGACGAGG ACGTGGACGT GGACGTCCCG GGCCCCGCGC CGAGATCTAT TCAGCGATGG 
TCGACCGCGC CCGGGCTCGT GGAGCCTCAG CCTCGGGATA ACGGCGCCTC GAAGGTATCA 
GTAGCTGATG TGCAATTTGG CCCGATGAGA TTTCATCAAG ACCAACTTCA GGTCCTGTTA 
GTGTTTACCA AAGAAGATAG CCAGTGTAAC GGATTCCACA GGGCATGTGA GAAAGCTGGG 
TTTAAGTGCA CAGTCACCAA GGAGGTGCAG ACTGTCCTTA CCTGTTTCCA GGACAAACTC 
CATGACATCA TCATCATAGA CCACAGATAT CCCCGACAGA TGGATGCAGA GACATTGTGC 
AGGTCTATCA GATCATCAAA ATTCTCAGAA AACACAGTTA TTGTTGGCGT AGTACGCAGG 
GTGGACAAAG AAGAATCATC TTTAATGCCC TTCCTTGCTG CTGGCTTCAC ACGGAGGTTT 
ATAGAGAACC CCAACGTCAT GGCCTGTTAT AATGAACTGC TCCAGTTGGC ATGTGGAGAG 
GTGCGATCAC AGCTGAAACT CAGGGCTTGT AACTCAGTAT TCACTGCATT AGAGAAAAGT 
CAAGAAGCAA TTGAAATTAC AAGTGAAGAC CACATTATAC AGTATGCAAA CCCTGCATTT 
GAATCAACAA TGGGTTATCA GTCAGGAGAA TTAATAGGGA AGGAGTTAGC ACAAGTGCCC 
ATAAATGAAA AGAAAGGTGA TTTGCTCGAT GCCATAAATT CGTGTGTCAC AGTAGACAAG 
GAATGGCAAG GAGTTTACCA TACCCAAAAG AAAAATGGAG ATAACATACA GCAAAATGTG 
AAGATAATAC CTGTCATTGG GCAGGGAGGA AAAATCAGAC ACTATGTGTC CATTATCAGA 
GTGTGCAATG GCAACAATAA GGTTGAGACC ACAACTGAGT GTGTTCAGAC TGACAGTCAA 
ACAGATAACC AGGCAGGCAA ACATAAGGAC AGGAGGAAAC ATTCGATGGA CGCCAAAGCG 
GTTTCCTCCA GAACAAGTGA CGTTTCCAGT CAGAGAAGAC ATTCCTCTTT GGCCCGGATA 
CATTCTATGA TGATTGAGGC ACCCATCACC AAGGTAATCA ATATTATCAA TGCTGCCCAG 
GAAAACAGTC CTGTGCCTGT GACGGAAGCC TTGAACCGAG TGCTGGATAT TCTGAGAACC 
ACTGAGCTGT ACTCGCCACA GTTTAATGCT CAGGATGATC CCCATGCCAC AGACCTCGTT 
GGGGGCTTAA TGTCTGATGG CTTACGAAGG TTTTCAGGAA ATGAGTATAT TCTTGCAACA 
AAAAACCTCC CACCACTTTC AAACAATTTG GCCACCCCCG TCTCCCTTCA TGATGTCCCA 
CCACGGATTG CCCTGGCCAT AGAAAATGAA GAACAGTGGG ACTTTGACAT TTTTGAGCTG 
GAAGTAGCAA CCCAAAATAG GCCTTTGATT TATCTAGGTC TGAAAACGTT TGCGCGTTTT 
GGAATGTGTG AGTTCTTACA GTGCTCTGAG ACAACGCTGA GATCTTGGTT TCAGATGATC 
GAATCCAACT ACCACTCTTC TAACCCCTAC CACAATTCCA CACACGCTGC GGATGTGCTT 
CACGCCACGG CGTATTTCCT TTCCAGAGAC AAAATAAAGG AAACTTTAGA TCGCATTGAC 
GAGGTTGCTG CCCTCATTGC GGCCACCGTC CATGACGTGG ATCACCCTGG GAGAACCAAC 
TCCTTCCTGT GCAACGCGGG AAACCAGCTG GCTGTGCTCT ACAACGACAC CGCTGTGCTG 
GAGAGCCACC ACGTGGCACT GGCCTTCCAG CTGACGCTTG AAAATGACCA GTGCAATATC 
TTTAAGCAAA TGGAGAGGAA TGACTACCGG ACACTACGCC AGAGCATTAT TGACATGGTC 
CTAGCCACAG AAATGACGAA GCACTTTGAA CATGTCAACA AATTTATCAA CAGCATCAAC 
AAGCCTTTGA CAGCCCAGGA GAGTGAGGAA CCTGACAGAA GTCTGGAAGA CATAAAAGCT 
ATGCTCAAGA CGCCAGAGAG CCGGGCTCTC ATCAAAAGAA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC GCTGGAGCAC TGCATCGAGT GGGCTGCGCG CATCTCAGAG 
GAGTATTTCT CTCAGACTGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCCGTG 
TTTGACAGAA ACACCTGCAG CATTCCCAAG TCTCAGATCT CCTTCATCGA TTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTC GTTGACCTGC CTAACCTTAT GCAGCACCTA 
GATGACAACT TCAGGTACTG GAAAGGACTG GATGAGAAGA AGCTTCGAAG CCTCCGCCCC 
CCTCCAGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PDE8 controls CD4
Cellular signalling4062-72(2017 Dec)
Basole CP,Nguyen RK,Lamothe K,Vang A,Clark R,Baillie GS,Epstein PM,Brocke S

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Inhibition of type 5 phosphodiesterase counteracts ?2-adrenergic signalling in beating cardiomyocytes.
Cardiovascular research106(3)408-20(2015 Jun)
Isidori AM,Cornacchione M,Barbagallo F,Di Grazia A,Barrios F,Fassina L,Monaco L,Giannetta E,Gianfrilli D,Garofalo S,Zhang X,Chen X,Xiang YK,Lenzi A,Pellegrini M,Naro F

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Phosphodiesterase-8A binds to and regulates Raf-1 kinase.
Proceedings of the National Academy of Sciences of the United States of America110(16)E1533-42(2013 Apr)
Brown KM,Day JP,Huston E,Zimmermann B,Hampel K,Christian F,Romano D,Terhzaz S,Lee LC,Willis MJ,Morton DB,Beavo JA,Shimizu-Albergine M,Davies SA,Kolch W,Houslay MD,Baillie GS

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Pde8a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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