Pde1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde1b
Entrez Gene ID	18574
Full Name	phosphodiesterase 1B, Ca2+-calmodulin dependent
Synonyms	Pde1b1
General protein information	Preferred Names phosphodiesterase 1B, Ca2+-calmodulin dependent Names calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B 63 kDa Cam-PDE cam-PDE 1B phosphodiesterase 1B, Ca2+-calmodulin dependent, 63 kDa phosphodiesterase 1B1, Ca2+-calmodulin dependent, 63 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030248407.1	XP_030104267.1	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform X1
NM_001357980.1	NP_001344909.1	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 3
NM_001285890.1	NP_001272819.1	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 2
XM_006520588.3	XP_006520651.3	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform X1
NM_008800.2	NP_032826.1	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PDE1B	NP_776840.1
Gallus gallus (chicken)	LOC777528	XP_001236974.3
Xenopus tropicalis (tropical clawed frog)	pde1b	NP_001090736.1
Homo sapiens (human)	PDE1B	NP_000915.1
Pan troglodytes (chimpanzee)	PDE1B	XP_509115.2
	PDE1B	XP_001110208.1
Canis lupus familiaris (dog)	PDE1B	XP_003640014.1
Mus musculus (house mouse)	Pde1b	NP_032826.1
Rattus norvegicus (Norway rat)	Pde1b	NP_073201.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphodiesterase-1b deletion confers depression-like behavioral resistance separate from stress-related effects in mice.
Hufgard JR, Williams MT, Vorhees CV
Genes, brain, and behavior16(8)756-767(2017 Nov)

Phosphodiesterase-1b (Pde1b) knockout mice are resistant to forced swim and tail suspension induced immobility and show upregulation of Pde10a.
Hufgard JR, Williams MT, Skelton MR, Grubisha O, Ferreira FM, Sanger H, Wright ME, Reed-Kessler TM, Rasmussen K, Duman RS, Vorhees CV
Psychopharmacology234(12)1803-1813(2017 Jun)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results prove that the kinetic properties of PDE isoforms play a major role in determining intracellular cAMP signals in response to physiological elevation of [Ca2+]i.
Title: Kinetic properties of Ca2+/calmodulin-dependent phosphodiesterase isoforms dictate intracellular cAMP dynamics in response to elevation of cytosolic Ca2+.

The present studies demonstrate increased striatal dopamine turnover in PDE1B knockout mice associated with increased baseline motor activity and an exaggerated locomotor response to dopaminergic stimulants such as methamphetamine and amphetamine.
Title: Behavioral and neurochemical characterization of mice deficient in the phosphodiesterase-1B (PDE1B) enzyme.

Data suggest a role for PDE1B in locomotor responses to psychostimulants through modulation of DARPP32-dependent pathways; however, this modulation does not necessarily impact other behaviors, such as anxiety or learning.
Title: Phosphodiesterase 1B differentially modulates the effects of methamphetamine on locomotor activity and spatial learning through DARPP32-dependent pathways: evidence from PDE1B-DARPP32 double-knockout mice.

knock-out mice exhibit locomotor hyperactivity in response to dopamine agonists and display impaired spatial learning
Title: Phosphodiesterase 1B knock-out mice exhibit exaggerated locomotor hyperactivity and DARPP-32 phosphorylation in response to dopamine agonists and display impaired spatial learning.

The following Pde1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02803	NM_008800.2 Latest version!	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu08966	NM_001285890.1 Latest version!	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu68410	XM_030248407.1 Latest version!	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu79058	NM_001357980.1 Latest version!	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu79058	XM_006520588.3 Latest version!	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde1b ( NM_008800.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032826.1 Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02803
Clone ID Related Accession (Same CDS sequence)	NM_008800.2
Accession Version	NM_008800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC058531.1, AC131721.4 and DN173601.1. On Oct 25, 2013 this sequence version replaced NM_008800.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.78933.1, SRR1660811.237885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTGT CCCCCCGCAG TCCTCCAGAG ATGCTGGAGT CGGATTGCCC GTCACCCCTG 
GAGCTGAAGT CAGCCCCCAG CAAGAAAATG TGGATTAAGC TACGGTCTCT GCTGCGCTAC 
ATGGTGAAGC AGTTGGAGAA TGGGGAGGTT AACATCGAGG AGCTGAAGAA AAACCTGGAA 
TACACAGCTT CCCTGCTGGA AGCTGTCTAC ATTGATGAGA CAAGGCAAAT CCTAGACACT 
GAAGATGAGC TTCGGGAACT TCGGTCAGAT GCTGTGCCTT CAGAGGTGCG GGACTGGTTG 
GCCTCCACCT TCACCCAGCA GACCCGAGCC AAAGGTCGCA GGGCAGAAGA GAAACCCAAG 
TTCCGAAGCA TCGTGCATGC TGTGCAGGCT GGGATCTTCG TGGAGCGGAT GTTCCGGAGA 
ACGTATACCT CTGTGGGCCC CACATACTCT ACTGCAGTCC ACAACTGTCT CAAGAACCTG 
GACCTCTGGT GCTTTGATGT CTTTTCCTTG AACCGGGCAG CCGATGACCA CGCTCTGAGG 
ACCATTGTTT TTGAGTTGCT GACTCGGCAT AGCCTCATCA GCCGCTTTAA GATTCCCACA 
GTGTTTCTGA TGAGTTTTCT GGAGGCCTTG GAGACAGGCT ATGGGAAATA TAAGAATCCT 
TACCACAACC AGATCCACGC AGCCGACGTG ACCCAGACTG TCCATTGCTT CCTTCTCCGC 
ACAGGCATGG TGCACTGCCT GTCAGAGATT GAGGTCTTGG CCATCATCTT TGCTGCAGCC 
ATCCATGACT ATGAGCACAC AGGCACAACC AACAGCTTCC ACATTCAGAC CAAGTCAGAA 
TGTGCCATCC TGTACAATGA TCGATCGGTG CTGGAGAATC ACCACATCAG CTCTGTCTTT 
CGAATGATGC AGGATGATGA GATGAACATT TTTATCAATC TCACCAAGGA TGAATTTGCA 
GAGCTGCGGG CCCTGGTTAT CGAGATGGTG TTGGCCACAG ACATGTCCTG CCATTTCCAG 
CAAGTGAAGA CTATGAAGAC AGCACTGCAG CAGTTGGAAA GGATTGACAA GTCCAAGGCC 
CTATCTCTTC TGCTTCATGC TGCTGACATC AGCCACCCAA CTAAGCAGTG GTCAGTCCAC 
AGCCGCTGGA CCAAGGCCCT AATGGAAGAG TTCTTCCGCC AGGGTGACAA GGAGGCAGAG 
CTGGGCCTGC CCTTCTCTCC ACTCTGTGAT CGCACTTCCA CATTGGTGGC CCAGTCCCAG 
ATAGGTTTCA TTGACTTCAT TGTGGAGCCA ACCTTCTCTG TGCTGACTGA TGTGGCAGAA 
AAGAGTGTCC AGCCCTTGGC AGATGATGAT TCCAAGCCTA AAAGTCAGCC CAGCTTCCAG 
TGGCGCCAGC CTTCTTTAGA TGTGGACGTA GGAGACCCCA ACCCTGATGT GGTCAGTTTC 
CGTGCCACCT GGACCAAGTA CATTCAAGAG AACAAACAGA AGTGGAAGGA ACGGGCAGCA 
AGTGGCATCA CCAACCAGAT GTCCATTGAT GAACTGTCCC CCTGTGAGGA AGAAGCCCCA 
TCCTCCCCTG CAGAAGATGA GCACAACCAG AATGGGAATC TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032826.1
CDS	353..1960
Translation	MELSPRSPPEMLESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEVNIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWLASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVHNCLKNLDLWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPTVFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIEVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVFRMMQDDEMNIFINLTKDEFAELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADDDSKPKSQPSFQWRQPSLDVDVGDPNPDVVSFRATWTKYIQENKQKWKERAASGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD

Target ORF information:

RefSeq Version	NM_008800.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008800.2

ORF Insert Sequence:

ATGGAGCTGT CCCCCCGCAG TCCTCCAGAG ATGCTGGAGT CGGATTGCCC GTCACCCCTG 
GAGCTGAAGT CAGCCCCCAG CAAGAAAATG TGGATTAAGC TACGGTCTCT GCTGCGCTAC 
ATGGTGAAGC AGTTGGAGAA TGGGGAGGTT AACATCGAGG AGCTGAAGAA AAACCTGGAA 
TACACAGCTT CCCTGCTGGA AGCTGTCTAC ATTGATGAGA CAAGGCAAAT CCTAGACACT 
GAAGATGAGC TTCGGGAACT TCGGTCAGAT GCTGTGCCTT CAGAGGTGCG GGACTGGTTG 
GCCTCCACCT TCACCCAGCA GACCCGAGCC AAAGGTCGCA GGGCAGAAGA GAAACCCAAG 
TTCCGAAGCA TCGTGCATGC TGTGCAGGCT GGGATCTTCG TGGAGCGGAT GTTCCGGAGA 
ACGTATACCT CTGTGGGCCC CACATACTCT ACTGCAGTCC ACAACTGTCT CAAGAACCTG 
GACCTCTGGT GCTTTGATGT CTTTTCCTTG AACCGGGCAG CCGATGACCA CGCTCTGAGG 
ACCATTGTTT TTGAGTTGCT GACTCGGCAT AGCCTCATCA GCCGCTTTAA GATTCCCACA 
GTGTTTCTGA TGAGTTTTCT GGAGGCCTTG GAGACAGGCT ATGGGAAATA TAAGAATCCT 
TACCACAACC AGATCCACGC AGCCGACGTG ACCCAGACTG TCCATTGCTT CCTTCTCCGC 
ACAGGCATGG TGCACTGCCT GTCAGAGATT GAGGTCTTGG CCATCATCTT TGCTGCAGCC 
ATCCATGACT ATGAGCACAC AGGCACAACC AACAGCTTCC ACATTCAGAC CAAGTCAGAA 
TGTGCCATCC TGTACAATGA TCGATCGGTG CTGGAGAATC ACCACATCAG CTCTGTCTTT 
CGAATGATGC AGGATGATGA GATGAACATT TTTATCAATC TCACCAAGGA TGAATTTGCA 
GAGCTGCGGG CCCTGGTTAT CGAGATGGTG TTGGCCACAG ACATGTCCTG CCATTTCCAG 
CAAGTGAAGA CTATGAAGAC AGCACTGCAG CAGTTGGAAA GGATTGACAA GTCCAAGGCC 
CTATCTCTTC TGCTTCATGC TGCTGACATC AGCCACCCAA CTAAGCAGTG GTCAGTCCAC 
AGCCGCTGGA CCAAGGCCCT AATGGAAGAG TTCTTCCGCC AGGGTGACAA GGAGGCAGAG 
CTGGGCCTGC CCTTCTCTCC ACTCTGTGAT CGCACTTCCA CATTGGTGGC CCAGTCCCAG 
ATAGGTTTCA TTGACTTCAT TGTGGAGCCA ACCTTCTCTG TGCTGACTGA TGTGGCAGAA 
AAGAGTGTCC AGCCCTTGGC AGATGATGAT TCCAAGCCTA AAAGTCAGCC CAGCTTCCAG 
TGGCGCCAGC CTTCTTTAGA TGTGGACGTA GGAGACCCCA ACCCTGATGT GGTCAGTTTC 
CGTGCCACCT GGACCAAGTA CATTCAAGAG AACAAACAGA AGTGGAAGGA ACGGGCAGCA 
AGTGGCATCA CCAACCAGAT GTCCATTGAT GAACTGTCCC CCTGTGAGGA AGAAGCCCCA 
TCCTCCCCTG CAGAAGATGA GCACAACCAG AATGGGAATC TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde1b ( NM_001285890.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272819.1 Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08966
Clone ID Related Accession (Same CDS sequence)	NM_001285890.1
Accession Version	NM_001285890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CV784321.1, AK004772.1, AC131721.4 and DN173601.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and contains an alternate 5' terminal exon compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.28811.1, SRR1660811.146562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAACC CTGTTCCTGT CCAGAGGAGC CACCTCCAGG GCCCCATCCT CAGGCTGCGC 
TACATGGTGA AGCAGTTGGA GAATGGGGAG GTTAACATCG AGGAGCTGAA GAAAAACCTG 
GAATACACAG CTTCCCTGCT GGAAGCTGTC TACATTGATG AGACAAGGCA AATCCTAGAC 
ACTGAAGATG AGCTTCGGGA ACTTCGGTCA GATGCTGTGC CTTCAGAGGT GCGGGACTGG 
TTGGCCTCCA CCTTCACCCA GCAGACCCGA GCCAAAGGTC GCAGGGCAGA AGAGAAACCC 
AAGTTCCGAA GCATCGTGCA TGCTGTGCAG GCTGGGATCT TCGTGGAGCG GATGTTCCGG 
AGAACGTATA CCTCTGTGGG CCCCACATAC TCTACTGCAG TCCACAACTG TCTCAAGAAC 
CTGGACCTCT GGTGCTTTGA TGTCTTTTCC TTGAACCGGG CAGCCGATGA CCACGCTCTG 
AGGACCATTG TTTTTGAGTT GCTGACTCGG CATAGCCTCA TCAGCCGCTT TAAGATTCCC 
ACAGTGTTTC TGATGAGTTT TCTGGAGGCC TTGGAGACAG GCTATGGGAA ATATAAGAAT 
CCTTACCACA ACCAGATCCA CGCAGCCGAC GTGACCCAGA CTGTCCATTG CTTCCTTCTC 
CGCACAGGCA TGGTGCACTG CCTGTCAGAG ATTGAGGTCT TGGCCATCAT CTTTGCTGCA 
GCCATCCATG ACTATGAGCA CACAGGCACA ACCAACAGCT TCCACATTCA GACCAAGTCA 
GAATGTGCCA TCCTGTACAA TGATCGATCG GTGCTGGAGA ATCACCACAT CAGCTCTGTC 
TTTCGAATGA TGCAGGATGA TGAGATGAAC ATTTTTATCA ATCTCACCAA GGATGAATTT 
GCAGAGCTGC GGGCCCTGGT TATCGAGATG GTGTTGGCCA CAGACATGTC CTGCCATTTC 
CAGCAAGTGA AGACTATGAA GACAGCACTG CAGCAGTTGG AAAGGATTGA CAAGTCCAAG 
GCCCTATCTC TTCTGCTTCA TGCTGCTGAC ATCAGCCACC CAACTAAGCA GTGGTCAGTC 
CACAGCCGCT GGACCAAGGC CCTAATGGAA GAGTTCTTCC GCCAGGGTGA CAAGGAGGCA 
GAGCTGGGCC TGCCCTTCTC TCCACTCTGT GATCGCACTT CCACATTGGT GGCCCAGTCC 
CAGATAGGTT TCATTGACTT CATTGTGGAG CCAACCTTCT CTGTGCTGAC TGATGTGGCA 
GAAAAGAGTG TCCAGCCCTT GGCAGATGAT GATTCCAAGC CTAAAAGTCA GCCCAGCTTC 
CAGTGGCGCC AGCCTTCTTT AGATGTGGAC GTAGGAGACC CCAACCCTGA TGTGGTCAGT 
TTCCGTGCCA CCTGGACCAA GTACATTCAA GAGAACAAAC AGAAGTGGAA GGAACGGGCA 
GCAAGTGGCA TCACCAACCA GATGTCCATT GATGAACTGT CCCCCTGTGA GGAAGAAGCC 
CCATCCTCCC CTGCAGAAGA TGAGCACAAC CAGAATGGGA ATCTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272819.1
CDS	393..1943
Translation	MANPVPVQRSHLQGPILRLRYMVKQLENGEVNIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWLASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVHNCLKNLDLWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPTVFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIEVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVFRMMQDDEMNIFINLTKDEFAELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADDDSKPKSQPSFQWRQPSLDVDVGDPNPDVVSFRATWTKYIQENKQKWKERAASGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD

Target ORF information:

RefSeq Version	NM_001285890.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285890.1

ORF Insert Sequence:

ATGGCAAACC CTGTTCCTGT CCAGAGGAGC CACCTCCAGG GCCCCATCCT CAGGCTGCGC 
TACATGGTGA AGCAGTTGGA GAATGGGGAG GTTAACATCG AGGAGCTGAA GAAAAACCTG 
GAATACACAG CTTCCCTGCT GGAAGCTGTC TACATTGATG AGACAAGGCA AATCCTAGAC 
ACTGAAGATG AGCTTCGGGA ACTTCGGTCA GATGCTGTGC CTTCAGAGGT GCGGGACTGG 
TTGGCCTCCA CCTTCACCCA GCAGACCCGA GCCAAAGGTC GCAGGGCAGA AGAGAAACCC 
AAGTTCCGAA GCATCGTGCA TGCTGTGCAG GCTGGGATCT TCGTGGAGCG GATGTTCCGG 
AGAACGTATA CCTCTGTGGG CCCCACATAC TCTACTGCAG TCCACAACTG TCTCAAGAAC 
CTGGACCTCT GGTGCTTTGA TGTCTTTTCC TTGAACCGGG CAGCCGATGA CCACGCTCTG 
AGGACCATTG TTTTTGAGTT GCTGACTCGG CATAGCCTCA TCAGCCGCTT TAAGATTCCC 
ACAGTGTTTC TGATGAGTTT TCTGGAGGCC TTGGAGACAG GCTATGGGAA ATATAAGAAT 
CCTTACCACA ACCAGATCCA CGCAGCCGAC GTGACCCAGA CTGTCCATTG CTTCCTTCTC 
CGCACAGGCA TGGTGCACTG CCTGTCAGAG ATTGAGGTCT TGGCCATCAT CTTTGCTGCA 
GCCATCCATG ACTATGAGCA CACAGGCACA ACCAACAGCT TCCACATTCA GACCAAGTCA 
GAATGTGCCA TCCTGTACAA TGATCGATCG GTGCTGGAGA ATCACCACAT CAGCTCTGTC 
TTTCGAATGA TGCAGGATGA TGAGATGAAC ATTTTTATCA ATCTCACCAA GGATGAATTT 
GCAGAGCTGC GGGCCCTGGT TATCGAGATG GTGTTGGCCA CAGACATGTC CTGCCATTTC 
CAGCAAGTGA AGACTATGAA GACAGCACTG CAGCAGTTGG AAAGGATTGA CAAGTCCAAG 
GCCCTATCTC TTCTGCTTCA TGCTGCTGAC ATCAGCCACC CAACTAAGCA GTGGTCAGTC 
CACAGCCGCT GGACCAAGGC CCTAATGGAA GAGTTCTTCC GCCAGGGTGA CAAGGAGGCA 
GAGCTGGGCC TGCCCTTCTC TCCACTCTGT GATCGCACTT CCACATTGGT GGCCCAGTCC 
CAGATAGGTT TCATTGACTT CATTGTGGAG CCAACCTTCT CTGTGCTGAC TGATGTGGCA 
GAAAAGAGTG TCCAGCCCTT GGCAGATGAT GATTCCAAGC CTAAAAGTCA GCCCAGCTTC 
CAGTGGCGCC AGCCTTCTTT AGATGTGGAC GTAGGAGACC CCAACCCTGA TGTGGTCAGT 
TTCCGTGCCA CCTGGACCAA GTACATTCAA GAGAACAAAC AGAAGTGGAA GGAACGGGCA 
GCAAGTGGCA TCACCAACCA GATGTCCATT GATGAACTGT CCCCCTGTGA GGAAGAAGCC 
CCATCCTCCC CTGCAGAAGA TGAGCACAAC CAGAATGGGA ATCTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde1b ( XM_030248407.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104267.1 Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68410
Clone ID Related Accession (Same CDS sequence)	XM_030248407.1
Accession Version	XM_030248407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC AGTTGGAGAA TGGGGAGGTT AACATCGAGG AGCTGAAGAA AAACCTGGAA 
TACACAGCTT CCCTGCTGGA AGCTGTCTAC ATTGATGAGA CAAGGCAAAT CCTAGACACT 
GAAGATGAGC TTCGGGAACT TCGGTCAGAT GCTGTGCCTT CAGAGGTGCG GGACTGGTTG 
GCCTCCACCT TCACCCAGCA GACCCGAGCC AAAGGTCGCA GGGCAGAAGA GAAACCCAAG 
TTCCGAAGCA TCGTGCATGC TGTGCAGGCT GGGATCTTCG TGGAGCGGAT GTTCCGGAGA 
ACGTATACCT CTGTGGGCCC CACATACTCT ACTGCAGTCC ACAACTGTCT CAAGAACCTG 
GACCTCTGGT GCTTTGATGT CTTTTCCTTG AACCGGGCAG CCGATGACCA CGCTCTGAGG 
ACCATTGTTT TTGAGTTGCT GACTCGGCAT AGCCTCATCA GCCGCTTTAA GATTCCCACA 
GTGTTTCTGA TGAGTTTTCT GGAGGCCTTG GAGACAGGCT ATGGGAAATA TAAGAATCCT 
TACCACAACC AGATCCACGC AGCCGACGTG ACCCAGACTG TCCATTGCTT CCTTCTCCGC 
ACAGGCATGG TGCACTGCCT GTCAGAGATT GAGGTCTTGG CCATCATCTT TGCTGCAGCC 
ATCCATGACT ATGAGCACAC AGGCACAACC AACAGCTTCC ACATTCAGAC CAAGTCAGAA 
TGTGCCATCC TGTACAATGA TCGATCGGTG CTGGAGAATC ACCACATCAG CTCTGTCTTT 
CGAATGATGC AGGATGATGA GATGAACATT TTTATCAATC TCACCAAGGA TGAATTTGCA 
GAGCTGCGGG CCCTGGTTAT CGAGATGGTG TTGGCCACAG ACATGTCCTG CCATTTCCAG 
CAAGTGAAGA CTATGAAGAC AGCACTGCAG CAGTTGGAAA GGATTGACAA GTCCAAGGCC 
CTATCTCTTC TGCTTCATGC TGCTGACATC AGCCACCCAA CTAAGCAGTG GTCAGTCCAC 
AGCCGCTGGA CCAAGGCCCT AATGGAAGAG TTCTTCCGCC AGGGTGACAA GGAGGCAGAG 
CTGGGCCTGC CCTTCTCTCC ACTCTGTGAT CGCACTTCCA CATTGGTGGC CCAGTCCCAG 
ATAGGTTTCA TTGACTTCAT TGTGGAGCCA ACCTTCTCTG TGCTGACTGA TGTGGCAGAA 
AAGAGTGTCC AGCCCTTGGC AGATGATGAT TCCAAGCCTA AAAGTCAGCC CAGCTTCCAG 
TGGCGCCAGC CTTCTTTAGA TGTGGACGTA GGAGACCCCA ACCCTGATGT GGTCAGTTTC 
CGTGCCACCT GGACCAAGTA CATTCAAGAG AACAAACAGA AGTGGAAGGA ACGGGCAGCA 
AGTGGCATCA CCAACCAGAT GTCCATTGAT GAACTGTCCC CCTGTGAGGA AGAAGCCCCA 
TCCTCCCCTG CAGAAGATGA GCACAACCAG AATGGGAATC TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104267.1
CDS	85..1572
Translation	MVKQLENGEVNIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWLASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVHNCLKNLDLWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPTVFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIEVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVFRMMQDDEMNIFINLTKDEFAELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADDDSKPKSQPSFQWRQPSLDVDVGDPNPDVVSFRATWTKYIQENKQKWKERAASGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD

Target ORF information:

RefSeq Version	XM_030248407.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248407.1

ORF Insert Sequence:

ATGGTGAAGC AGTTGGAGAA TGGGGAGGTT AACATCGAGG AGCTGAAGAA AAACCTGGAA 
TACACAGCTT CCCTGCTGGA AGCTGTCTAC ATTGATGAGA CAAGGCAAAT CCTAGACACT 
GAAGATGAGC TTCGGGAACT TCGGTCAGAT GCTGTGCCTT CAGAGGTGCG GGACTGGTTG 
GCCTCCACCT TCACCCAGCA GACCCGAGCC AAAGGTCGCA GGGCAGAAGA GAAACCCAAG 
TTCCGAAGCA TCGTGCATGC TGTGCAGGCT GGGATCTTCG TGGAGCGGAT GTTCCGGAGA 
ACGTATACCT CTGTGGGCCC CACATACTCT ACTGCAGTCC ACAACTGTCT CAAGAACCTG 
GACCTCTGGT GCTTTGATGT CTTTTCCTTG AACCGGGCAG CCGATGACCA CGCTCTGAGG 
ACCATTGTTT TTGAGTTGCT GACTCGGCAT AGCCTCATCA GCCGCTTTAA GATTCCCACA 
GTGTTTCTGA TGAGTTTTCT GGAGGCCTTG GAGACAGGCT ATGGGAAATA TAAGAATCCT 
TACCACAACC AGATCCACGC AGCCGACGTG ACCCAGACTG TCCATTGCTT CCTTCTCCGC 
ACAGGCATGG TGCACTGCCT GTCAGAGATT GAGGTCTTGG CCATCATCTT TGCTGCAGCC 
ATCCATGACT ATGAGCACAC AGGCACAACC AACAGCTTCC ACATTCAGAC CAAGTCAGAA 
TGTGCCATCC TGTACAATGA TCGATCGGTG CTGGAGAATC ACCACATCAG CTCTGTCTTT 
CGAATGATGC AGGATGATGA GATGAACATT TTTATCAATC TCACCAAGGA TGAATTTGCA 
GAGCTGCGGG CCCTGGTTAT CGAGATGGTG TTGGCCACAG ACATGTCCTG CCATTTCCAG 
CAAGTGAAGA CTATGAAGAC AGCACTGCAG CAGTTGGAAA GGATTGACAA GTCCAAGGCC 
CTATCTCTTC TGCTTCATGC TGCTGACATC AGCCACCCAA CTAAGCAGTG GTCAGTCCAC 
AGCCGCTGGA CCAAGGCCCT AATGGAAGAG TTCTTCCGCC AGGGTGACAA GGAGGCAGAG 
CTGGGCCTGC CCTTCTCTCC ACTCTGTGAT CGCACTTCCA CATTGGTGGC CCAGTCCCAG 
ATAGGTTTCA TTGACTTCAT TGTGGAGCCA ACCTTCTCTG TGCTGACTGA TGTGGCAGAA 
AAGAGTGTCC AGCCCTTGGC AGATGATGAT TCCAAGCCTA AAAGTCAGCC CAGCTTCCAG 
TGGCGCCAGC CTTCTTTAGA TGTGGACGTA GGAGACCCCA ACCCTGATGT GGTCAGTTTC 
CGTGCCACCT GGACCAAGTA CATTCAAGAG AACAAACAGA AGTGGAAGGA ACGGGCAGCA 
AGTGGCATCA CCAACCAGAT GTCCATTGAT GAACTGTCCC CCTGTGAGGA AGAAGCCCCA 
TCCTCCCCTG CAGAAGATGA GCACAACCAG AATGGGAATC TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde1b ( NM_001357980.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344909.1 Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 3, mRNA.

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CloneID	OMu79058
Clone ID Related Accession (Same CDS sequence)	NM_001357980.1 , XM_006520588.3
Accession Version	NM_001357980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131721.4. On Nov 14, 2017 this sequence version replaced XM_006520588.3. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and contains an alternate 5' terminal exon compared to variant 1. The encoded protein (isoform 3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.143928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTACA TAGATATAAA GGCTGTGTTT CGTGTGAACA AGAGACTGAA ACTGCTGCGC 
TACATGGTGA AGCAGTTGGA GAATGGGGAG GTTAACATCG AGGAGCTGAA GAAAAACCTG 
GAATACACAG CTTCCCTGCT GGAAGCTGTC TACATTGATG AGACAAGGCA AATCCTAGAC 
ACTGAAGATG AGCTTCGGGA ACTTCGGTCA GATGCTGTGC CTTCAGAGGT GCGGGACTGG 
TTGGCCTCCA CCTTCACCCA GCAGACCCGA GCCAAAGGTC GCAGGGCAGA AGAGAAACCC 
AAGTTCCGAA GCATCGTGCA TGCTGTGCAG GCTGGGATCT TCGTGGAGCG GATGTTCCGG 
AGAACGTATA CCTCTGTGGG CCCCACATAC TCTACTGCAG TCCACAACTG TCTCAAGAAC 
CTGGACCTCT GGTGCTTTGA TGTCTTTTCC TTGAACCGGG CAGCCGATGA CCACGCTCTG 
AGGACCATTG TTTTTGAGTT GCTGACTCGG CATAGCCTCA TCAGCCGCTT TAAGATTCCC 
ACAGTGTTTC TGATGAGTTT TCTGGAGGCC TTGGAGACAG GCTATGGGAA ATATAAGAAT 
CCTTACCACA ACCAGATCCA CGCAGCCGAC GTGACCCAGA CTGTCCATTG CTTCCTTCTC 
CGCACAGGCA TGGTGCACTG CCTGTCAGAG ATTGAGGTCT TGGCCATCAT CTTTGCTGCA 
GCCATCCATG ACTATGAGCA CACAGGCACA ACCAACAGCT TCCACATTCA GACCAAGTCA 
GAATGTGCCA TCCTGTACAA TGATCGATCG GTGCTGGAGA ATCACCACAT CAGCTCTGTC 
TTTCGAATGA TGCAGGATGA TGAGATGAAC ATTTTTATCA ATCTCACCAA GGATGAATTT 
GCAGAGCTGC GGGCCCTGGT TATCGAGATG GTGTTGGCCA CAGACATGTC CTGCCATTTC 
CAGCAAGTGA AGACTATGAA GACAGCACTG CAGCAGTTGG AAAGGATTGA CAAGTCCAAG 
GCCCTATCTC TTCTGCTTCA TGCTGCTGAC ATCAGCCACC CAACTAAGCA GTGGTCAGTC 
CACAGCCGCT GGACCAAGGC CCTAATGGAA GAGTTCTTCC GCCAGGGTGA CAAGGAGGCA 
GAGCTGGGCC TGCCCTTCTC TCCACTCTGT GATCGCACTT CCACATTGGT GGCCCAGTCC 
CAGATAGGTT TCATTGACTT CATTGTGGAG CCAACCTTCT CTGTGCTGAC TGATGTGGCA 
GAAAAGAGTG TCCAGCCCTT GGCAGATGAT GATTCCAAGC CTAAAAGTCA GCCCAGCTTC 
CAGTGGCGCC AGCCTTCTTT AGATGTGGAC GTAGGAGACC CCAACCCTGA TGTGGTCAGT 
TTCCGTGCCA CCTGGACCAA GTACATTCAA GAGAACAAAC AGAAGTGGAA GGAACGGGCA 
GCAAGTGGCA TCACCAACCA GATGTCCATT GATGAACTGT CCCCCTGTGA GGAAGAAGCC 
CCATCCTCCC CTGCAGAAGA TGAGCACAAC CAGAATGGGA ATCTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344909.1
CDS	16..1566
Translation	MSYIDIKAVFRVNKRLKLLRYMVKQLENGEVNIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWLASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVHNCLKNLDLWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPTVFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIEVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVFRMMQDDEMNIFINLTKDEFAELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADDDSKPKSQPSFQWRQPSLDVDVGDPNPDVVSFRATWTKYIQENKQKWKERAASGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD

Target ORF information:

RefSeq Version	NM_001357980.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357980.1

ORF Insert Sequence:

ATGAGTTACA TAGATATAAA GGCTGTGTTT CGTGTGAACA AGAGACTGAA ACTGCTGCGC 
TACATGGTGA AGCAGTTGGA GAATGGGGAG GTTAACATCG AGGAGCTGAA GAAAAACCTG 
GAATACACAG CTTCCCTGCT GGAAGCTGTC TACATTGATG AGACAAGGCA AATCCTAGAC 
ACTGAAGATG AGCTTCGGGA ACTTCGGTCA GATGCTGTGC CTTCAGAGGT GCGGGACTGG 
TTGGCCTCCA CCTTCACCCA GCAGACCCGA GCCAAAGGTC GCAGGGCAGA AGAGAAACCC 
AAGTTCCGAA GCATCGTGCA TGCTGTGCAG GCTGGGATCT TCGTGGAGCG GATGTTCCGG 
AGAACGTATA CCTCTGTGGG CCCCACATAC TCTACTGCAG TCCACAACTG TCTCAAGAAC 
CTGGACCTCT GGTGCTTTGA TGTCTTTTCC TTGAACCGGG CAGCCGATGA CCACGCTCTG 
AGGACCATTG TTTTTGAGTT GCTGACTCGG CATAGCCTCA TCAGCCGCTT TAAGATTCCC 
ACAGTGTTTC TGATGAGTTT TCTGGAGGCC TTGGAGACAG GCTATGGGAA ATATAAGAAT 
CCTTACCACA ACCAGATCCA CGCAGCCGAC GTGACCCAGA CTGTCCATTG CTTCCTTCTC 
CGCACAGGCA TGGTGCACTG CCTGTCAGAG ATTGAGGTCT TGGCCATCAT CTTTGCTGCA 
GCCATCCATG ACTATGAGCA CACAGGCACA ACCAACAGCT TCCACATTCA GACCAAGTCA 
GAATGTGCCA TCCTGTACAA TGATCGATCG GTGCTGGAGA ATCACCACAT CAGCTCTGTC 
TTTCGAATGA TGCAGGATGA TGAGATGAAC ATTTTTATCA ATCTCACCAA GGATGAATTT 
GCAGAGCTGC GGGCCCTGGT TATCGAGATG GTGTTGGCCA CAGACATGTC CTGCCATTTC 
CAGCAAGTGA AGACTATGAA GACAGCACTG CAGCAGTTGG AAAGGATTGA CAAGTCCAAG 
GCCCTATCTC TTCTGCTTCA TGCTGCTGAC ATCAGCCACC CAACTAAGCA GTGGTCAGTC 
CACAGCCGCT GGACCAAGGC CCTAATGGAA GAGTTCTTCC GCCAGGGTGA CAAGGAGGCA 
GAGCTGGGCC TGCCCTTCTC TCCACTCTGT GATCGCACTT CCACATTGGT GGCCCAGTCC 
CAGATAGGTT TCATTGACTT CATTGTGGAG CCAACCTTCT CTGTGCTGAC TGATGTGGCA 
GAAAAGAGTG TCCAGCCCTT GGCAGATGAT GATTCCAAGC CTAAAAGTCA GCCCAGCTTC 
CAGTGGCGCC AGCCTTCTTT AGATGTGGAC GTAGGAGACC CCAACCCTGA TGTGGTCAGT 
TTCCGTGCCA CCTGGACCAA GTACATTCAA GAGAACAAAC AGAAGTGGAA GGAACGGGCA 
GCAAGTGGCA TCACCAACCA GATGTCCATT GATGAACTGT CCCCCTGTGA GGAAGAAGCC 
CCATCCTCCC CTGCAGAAGA TGAGCACAAC CAGAATGGGA ATCTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde1b ( XM_006520588.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520651.3 Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79058
Clone ID Related Accession (Same CDS sequence)	NM_001357980.1 , XM_006520588.3
Accession Version	XM_006520588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACA TAGATATAAA GGCTGTGTTT CGTGTGAACA AGAGACTGAA ACTGCTGCGC 
TACATGGTGA AGCAGTTGGA GAATGGGGAG GTTAACATCG AGGAGCTGAA GAAAAACCTG 
GAATACACAG CTTCCCTGCT GGAAGCTGTC TACATTGATG AGACAAGGCA AATCCTAGAC 
ACTGAAGATG AGCTTCGGGA ACTTCGGTCA GATGCTGTGC CTTCAGAGGT GCGGGACTGG 
TTGGCCTCCA CCTTCACCCA GCAGACCCGA GCCAAAGGTC GCAGGGCAGA AGAGAAACCC 
AAGTTCCGAA GCATCGTGCA TGCTGTGCAG GCTGGGATCT TCGTGGAGCG GATGTTCCGG 
AGAACGTATA CCTCTGTGGG CCCCACATAC TCTACTGCAG TCCACAACTG TCTCAAGAAC 
CTGGACCTCT GGTGCTTTGA TGTCTTTTCC TTGAACCGGG CAGCCGATGA CCACGCTCTG 
AGGACCATTG TTTTTGAGTT GCTGACTCGG CATAGCCTCA TCAGCCGCTT TAAGATTCCC 
ACAGTGTTTC TGATGAGTTT TCTGGAGGCC TTGGAGACAG GCTATGGGAA ATATAAGAAT 
CCTTACCACA ACCAGATCCA CGCAGCCGAC GTGACCCAGA CTGTCCATTG CTTCCTTCTC 
CGCACAGGCA TGGTGCACTG CCTGTCAGAG ATTGAGGTCT TGGCCATCAT CTTTGCTGCA 
GCCATCCATG ACTATGAGCA CACAGGCACA ACCAACAGCT TCCACATTCA GACCAAGTCA 
GAATGTGCCA TCCTGTACAA TGATCGATCG GTGCTGGAGA ATCACCACAT CAGCTCTGTC 
TTTCGAATGA TGCAGGATGA TGAGATGAAC ATTTTTATCA ATCTCACCAA GGATGAATTT 
GCAGAGCTGC GGGCCCTGGT TATCGAGATG GTGTTGGCCA CAGACATGTC CTGCCATTTC 
CAGCAAGTGA AGACTATGAA GACAGCACTG CAGCAGTTGG AAAGGATTGA CAAGTCCAAG 
GCCCTATCTC TTCTGCTTCA TGCTGCTGAC ATCAGCCACC CAACTAAGCA GTGGTCAGTC 
CACAGCCGCT GGACCAAGGC CCTAATGGAA GAGTTCTTCC GCCAGGGTGA CAAGGAGGCA 
GAGCTGGGCC TGCCCTTCTC TCCACTCTGT GATCGCACTT CCACATTGGT GGCCCAGTCC 
CAGATAGGTT TCATTGACTT CATTGTGGAG CCAACCTTCT CTGTGCTGAC TGATGTGGCA 
GAAAAGAGTG TCCAGCCCTT GGCAGATGAT GATTCCAAGC CTAAAAGTCA GCCCAGCTTC 
CAGTGGCGCC AGCCTTCTTT AGATGTGGAC GTAGGAGACC CCAACCCTGA TGTGGTCAGT 
TTCCGTGCCA CCTGGACCAA GTACATTCAA GAGAACAAAC AGAAGTGGAA GGAACGGGCA 
GCAAGTGGCA TCACCAACCA GATGTCCATT GATGAACTGT CCCCCTGTGA GGAAGAAGCC 
CCATCCTCCC CTGCAGAAGA TGAGCACAAC CAGAATGGGA ATCTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520651.3
CDS	11..1561
Translation	MSYIDIKAVFRVNKRLKLLRYMVKQLENGEVNIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWLASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVHNCLKNLDLWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPTVFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIEVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVFRMMQDDEMNIFINLTKDEFAELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADDDSKPKSQPSFQWRQPSLDVDVGDPNPDVVSFRATWTKYIQENKQKWKERAASGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD

Target ORF information:

RefSeq Version	XM_006520588.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520588.3

ORF Insert Sequence:

ATGAGTTACA TAGATATAAA GGCTGTGTTT CGTGTGAACA AGAGACTGAA ACTGCTGCGC 
TACATGGTGA AGCAGTTGGA GAATGGGGAG GTTAACATCG AGGAGCTGAA GAAAAACCTG 
GAATACACAG CTTCCCTGCT GGAAGCTGTC TACATTGATG AGACAAGGCA AATCCTAGAC 
ACTGAAGATG AGCTTCGGGA ACTTCGGTCA GATGCTGTGC CTTCAGAGGT GCGGGACTGG 
TTGGCCTCCA CCTTCACCCA GCAGACCCGA GCCAAAGGTC GCAGGGCAGA AGAGAAACCC 
AAGTTCCGAA GCATCGTGCA TGCTGTGCAG GCTGGGATCT TCGTGGAGCG GATGTTCCGG 
AGAACGTATA CCTCTGTGGG CCCCACATAC TCTACTGCAG TCCACAACTG TCTCAAGAAC 
CTGGACCTCT GGTGCTTTGA TGTCTTTTCC TTGAACCGGG CAGCCGATGA CCACGCTCTG 
AGGACCATTG TTTTTGAGTT GCTGACTCGG CATAGCCTCA TCAGCCGCTT TAAGATTCCC 
ACAGTGTTTC TGATGAGTTT TCTGGAGGCC TTGGAGACAG GCTATGGGAA ATATAAGAAT 
CCTTACCACA ACCAGATCCA CGCAGCCGAC GTGACCCAGA CTGTCCATTG CTTCCTTCTC 
CGCACAGGCA TGGTGCACTG CCTGTCAGAG ATTGAGGTCT TGGCCATCAT CTTTGCTGCA 
GCCATCCATG ACTATGAGCA CACAGGCACA ACCAACAGCT TCCACATTCA GACCAAGTCA 
GAATGTGCCA TCCTGTACAA TGATCGATCG GTGCTGGAGA ATCACCACAT CAGCTCTGTC 
TTTCGAATGA TGCAGGATGA TGAGATGAAC ATTTTTATCA ATCTCACCAA GGATGAATTT 
GCAGAGCTGC GGGCCCTGGT TATCGAGATG GTGTTGGCCA CAGACATGTC CTGCCATTTC 
CAGCAAGTGA AGACTATGAA GACAGCACTG CAGCAGTTGG AAAGGATTGA CAAGTCCAAG 
GCCCTATCTC TTCTGCTTCA TGCTGCTGAC ATCAGCCACC CAACTAAGCA GTGGTCAGTC 
CACAGCCGCT GGACCAAGGC CCTAATGGAA GAGTTCTTCC GCCAGGGTGA CAAGGAGGCA 
GAGCTGGGCC TGCCCTTCTC TCCACTCTGT GATCGCACTT CCACATTGGT GGCCCAGTCC 
CAGATAGGTT TCATTGACTT CATTGTGGAG CCAACCTTCT CTGTGCTGAC TGATGTGGCA 
GAAAAGAGTG TCCAGCCCTT GGCAGATGAT GATTCCAAGC CTAAAAGTCA GCCCAGCTTC 
CAGTGGCGCC AGCCTTCTTT AGATGTGGAC GTAGGAGACC CCAACCCTGA TGTGGTCAGT 
TTCCGTGCCA CCTGGACCAA GTACATTCAA GAGAACAAAC AGAAGTGGAA GGAACGGGCA 
GCAAGTGGCA TCACCAACCA GATGTCCATT GATGAACTGT CCCCCTGTGA GGAAGAAGCC 
CCATCCTCCC CTGCAGAAGA TGAGCACAAC CAGAATGGGA ATCTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphodiesterase-1b deletion confers depression-like behavioral resistance separate from stress-related effects in mice.
Genes, brain, and behavior16(8)756-767(2017 Nov)
Hufgard JR,Williams MT,Vorhees CV

Phosphodiesterase-1b (Pde1b) knockout mice are resistant to forced swim and tail suspension induced immobility and show upregulation of Pde10a.
Psychopharmacology234(12)1803-1813(2017 Jun)
Hufgard JR,Williams MT,Skelton MR,Grubisha O,Ferreira FM,Sanger H,Wright ME,Reed-Kessler TM,Rasmussen K,Duman RS,Vorhees CV

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Pde1b cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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