Pcx cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcx
Entrez Gene ID	18563
Full Name	pyruvate carboxylase
Synonyms	Pc,Pcb
General protein information	Preferred Names pyruvate carboxylase Names pyruvate carboxylase, mitochondrial pyruvate decarboxylase pyruvic carboxylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 4.07 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008797.3	NP_032823.2	pyruvate carboxylase, mitochondrial isoform 2
NM_001162946.1	NP_001156418.1	pyruvate carboxylase, mitochondrial isoform 1
XM_006531678.3	XP_006531741.1	pyruvate carboxylase, mitochondrial isoform X1
XM_006531678.4	XP_006531741.1	pyruvate carboxylase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PC	NP_071504.2
Canis lupus familiaris (dog)	PC	XP_540825.2
Bos taurus (cattle)	PC	NP_808815.2
Mus musculus (house mouse)	Pcx	NP_001156418.1
Caenorhabditis elegans (roundworm)	pyc-1	NP_001256376.1
Saccharomyces cerevisiae (baker's yeast)	PYC2	NP_009777.1
Kluyveromyces lactis	KLLA0C05764g	XP_452456.1
Eremothecium gossypii	AGOS_AAR162C	NP_982705.2
Magnaporthe oryzae (rice blast fungus)	MGG_07756	XP_003712969.1
Pan troglodytes (chimpanzee)	PC	XP_003952003.1
Neurospora crassa	NCU02505	XP_965636.1
Xenopus tropicalis (tropical clawed frog)	pc.2	NP_001096495.1
Schizosaccharomyces pombe (fission yeast)	pyr1	NP_595900.1
	PC	XP_001107814.1
Rattus norvegicus (Norway rat)	Pc	NP_036876.2
Danio rerio (zebrafish)	pcxb	NP_571625.1
Drosophila melanogaster (fruit fly)	CG1516	NP_724844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004742	XP_001689096.1
Saccharomyces cerevisiae (baker's yeast)	PYC1	NP_011453.1

Gene Ontology
Bibliography

Related articles in PubMed

Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro.
Harrison SE, Sozen B, Christodoulou N, Kyprianou C, Zernicka-Goetz M
Science (New York, N.Y.)356(6334)(2017 04)

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI
The Journal of cell biology216(3)675-693(2017 Mar)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Reduction of islet pyruvate carboxylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice.
Han J, Liu YQ
The Journal of endocrinology204(2)143-52(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that PC-mediated anaplerosis in early-stage NSCLC is required for tumor survival and proliferation.
Title: Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation.

TR4-mediated enhancement of fatty acid synthesis in adipocytes requires increased expression of PC gene
Title: TR4 promotes fatty acid synthesis in 3T3-L1 adipocytes by activation of pyruvate carboxylase expression.

Repression of pyruvate carboxylase could be interpreted as a survival tactic of repressing anabolic pathways to support a redirection of limited carbon units to catabolism rather than storage
Title: Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction.

Three GC boxes within the proximal promoter of the murine pyruvate carboxylase gene bind Sp1/Sp3
Title: Functional characterization of the proximal promoter of the murine pyruvate carboxylase gene in hepatocytes: role of multiple gc boxes.

CREB bind to -1639/-1631 cyclic AMP responsive element of mouse PC gene in vitro and in vivo, respectively.
Title: Identification of the cyclic AMP responsive element (CRE) that mediates transcriptional regulation of the pyruvate carboxylase gene in HepG2 cells.

The following Pcx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15330	XM_006531678.4 Latest version!	Mus musculus pyruvate carboxylase (Pcx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13237	NM_001162946.1 Latest version!	Mus musculus pyruvate carboxylase (Pcx), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$587.30 $839.00
OMu15330	NM_008797.3 Latest version!	Mus musculus pyruvate carboxylase (Pcx), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu15330	XM_006531678.3	Mus musculus pyruvate carboxylase (Pcx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcx ( XM_006531678.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531741.1 Mus musculus pyruvate carboxylase (Pcx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15330
Clone ID Related Accession (Same CDS sequence)	NM_008797.3 , XM_006531678.3 , XM_006531678.4
Accession Version	XM_006531678.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531678.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
TCGGCCCCTG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ATAAGCCTAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCGTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACAG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGCCTG GCACCTGTGC AGGCCTACCT GCACATTCCA 
GACATCATCA AGGTGGCCAA GGAAAATGGT GTAGATGCGG TGCATCCTGG CTATGGGTTC 
CTCTCAGAGC GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG GTTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATC 
GCTGCAGGCG TTCCAGTGGT CCCCGGCACG GACTCCCCCA TCAGCTCCCT GCACGAGGCG 
CATGAGTTCT CCAACACCTA CGGCTTCCCT ATTATCTTCA AGGCCGCCTA CGGAGGTGGG 
GGCCGCGGCA TGCGGGTCGT GCATAGCTAT GAGGAGTTGG AAGAGAATTA CACCCGGGCC 
TACTCCGAGG CCTTGGCAGC CTTTGGGAAT GGAGCGTTGT TTGTGGAGAA GTTCATTGAG 
AAGCCAAGGC ACATTGAGGT GCAGATCCTA GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGTCGG CACCAGAAGG TGGTAGAGAT CGCCCCTGCT 
ACCCACCTGG ATCCCCAACT TCGCTCACGT CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTAGGCT ATGAGAACGC CGGCACTGTG GAGTTCCTGG TGGACAAGCA CGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACCGA GGAGATCACA 
GATGTGGACC TGGTCCATGC TCAGATCCAC GTGTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAT GGCTGTGCCA TTCAGTGTCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACCGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCATTCC AGGGCGCTGT CATATCGCCC 
CACTATGACT CTCTGCTCGT CAAGGTCATT GCACACGGCA AAGACCACCC CACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTGGCAG GCACAGTGGA CACCCAGTTC 
ATCGATGAGA ACCCTGAGCT GTTCCAGCTT CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TCGGACATGT CATGGTGAAT GGCCCTACCA CTCCAATCCC TGTCAATGTG 
AGCCCCAGTC CTGTGGATCC TGCTGTTCCT GTGGTGCCCA TAGGCCCACC TCCAGCTGGT 
TTCAGGGACA TCCTTCTGCG AGAAGGGCCA GAGGGCTTTG CCCGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGTTGATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTGGCCACT 
AGAGTGCGCA CACATGATCT CAAAAAGATT GCGCCCTATG TTGCCCACAA CTTCAACAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGCGCC ACGTTCGACG TTGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTTA TCCCGAACAT CCCGTTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGT ATGGACGTCT TCCGAGTCTT TGACTCCCTC 
AACTACTTGC CAAACATGCT GCTGGGCATG GAAGCAGCAG GCAGTGCTGG GGGTGTGGTG 
GAGGCTGCCA TCTCATACAC GGGGGACGTG GCTGACCCTA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCTG GCACTCACAT CCTGTGCATT 
AAGGACATGG CGGGCCTGCT GAAGCCTGCC GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGATTCC CCGACCTCCC ACTGCACATC CATACCCATG ATACATCAGG GGCAGGTGTG 
GCAGCCATGC TGGCCTGTGC ACAAGCAGGG GCTGATGTTG TGGACGTGGC AGTAGACTCC 
ATGTCTGGGA TGACCTCACA GCCAAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTTTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA CTGGGAAGGG 
GCTCGGGGAC TGTACGCAGC CTTCGATTGC ACGGCTACCA TGAAGTCTGG CAACTCCGAC 
GTGTATGAGA ATGAGATTCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCATAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ATGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAGCCCTTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGGGAG 
GAGGTGACCC CAGAGGACGT CCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAATTC 
AAAGACTTCA CGGCTACCTT CGGCCCCCTG GATAGCCTTA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CCTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CTTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCGATGCC TGGGAAGGTC 
ATAGACATCA AGGTGGCAGC AGGGGACAAG GTGGCTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CATGTTACCA AGGACATGAC TCTGGAAGGC GACGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531741.1
CDS	160..3696
Translation	MLKFQTVRGGLRLLGVRRSSSAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDSPISSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVNVSPSPVDPAVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGDKVAKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006531678.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate carboxylase (Pcx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531678.4

ORF Insert Sequence:

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
TCGGCCCCTG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ATAAGCCTAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCGTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACAG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGCCTG GCACCTGTGC AGGCCTACCT GCACATTCCA 
GACATCATCA AGGTGGCCAA GGAAAATGGT GTAGATGCGG TGCATCCTGG CTATGGGTTC 
CTCTCAGAGC GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG GTTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATC 
GCTGCAGGCG TTCCAGTGGT CCCCGGCACG GACTCCCCCA TCAGCTCCCT GCACGAGGCG 
CATGAGTTCT CCAACACCTA CGGCTTCCCT ATTATCTTCA AGGCCGCCTA CGGAGGTGGG 
GGCCGCGGCA TGCGGGTCGT GCATAGCTAT GAGGAGTTGG AAGAGAATTA CACCCGGGCC 
TACTCCGAGG CCTTGGCAGC CTTTGGGAAT GGAGCGTTGT TTGTGGAGAA GTTCATTGAG 
AAGCCAAGGC ACATTGAGGT GCAGATCCTA GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGTCGG CACCAGAAGG TGGTAGAGAT CGCCCCTGCT 
ACCCACCTGG ATCCCCAACT TCGCTCACGT CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTAGGCT ATGAGAACGC CGGCACTGTG GAGTTCCTGG TGGACAAGCA CGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACCGA GGAGATCACA 
GATGTGGACC TGGTCCATGC TCAGATCCAC GTGTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAT GGCTGTGCCA TTCAGTGTCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACCGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCATTCC AGGGCGCTGT CATATCGCCC 
CACTATGACT CTCTGCTCGT CAAGGTCATT GCACACGGCA AAGACCACCC CACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTGGCAG GCACAGTGGA CACCCAGTTC 
ATCGATGAGA ACCCTGAGCT GTTCCAGCTT CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TCGGACATGT CATGGTGAAT GGCCCTACCA CTCCAATCCC TGTCAATGTG 
AGCCCCAGTC CTGTGGATCC TGCTGTTCCT GTGGTGCCCA TAGGCCCACC TCCAGCTGGT 
TTCAGGGACA TCCTTCTGCG AGAAGGGCCA GAGGGCTTTG CCCGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGTTGATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTGGCCACT 
AGAGTGCGCA CACATGATCT CAAAAAGATT GCGCCCTATG TTGCCCACAA CTTCAACAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGCGCC ACGTTCGACG TTGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTTA TCCCGAACAT CCCGTTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGT ATGGACGTCT TCCGAGTCTT TGACTCCCTC 
AACTACTTGC CAAACATGCT GCTGGGCATG GAAGCAGCAG GCAGTGCTGG GGGTGTGGTG 
GAGGCTGCCA TCTCATACAC GGGGGACGTG GCTGACCCTA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCTG GCACTCACAT CCTGTGCATT 
AAGGACATGG CGGGCCTGCT GAAGCCTGCC GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGATTCC CCGACCTCCC ACTGCACATC CATACCCATG ATACATCAGG GGCAGGTGTG 
GCAGCCATGC TGGCCTGTGC ACAAGCAGGG GCTGATGTTG TGGACGTGGC AGTAGACTCC 
ATGTCTGGGA TGACCTCACA GCCAAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTTTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA CTGGGAAGGG 
GCTCGGGGAC TGTACGCAGC CTTCGATTGC ACGGCTACCA TGAAGTCTGG CAACTCCGAC 
GTGTATGAGA ATGAGATTCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCATAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ATGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAGCCCTTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGGGAG 
GAGGTGACCC CAGAGGACGT CCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAATTC 
AAAGACTTCA CGGCTACCTT CGGCCCCCTG GATAGCCTTA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CCTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CTTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCGATGCC TGGGAAGGTC 
ATAGACATCA AGGTGGCAGC AGGGGACAAG GTGGCTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CATGTTACCA AGGACATGAC TCTGGAAGGC GACGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcx ( NM_001162946.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156418.1 Mus musculus pyruvate carboxylase (Pcx), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13237
Clone ID Related Accession (Same CDS sequence)	NM_001162946.1
Accession Version	NM_001162946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	pyruvate carboxylase, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY293312.1, BY230152.1, BG087228.2, AK148328.1, AK077665.1 and AA619324.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148328.1, AK170871.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGCTGA AGTTCCAAAC AGTTCGAGGG GGCCTGAGGC TCCTGGGTGT CCGCCGATCC 
TCCTCGGCCC CTGTTGCCTC CCCAAATGTC CGGCGTCTGG AGTATAAGCC TATCAAGAAA 
GTAATGGTGG CCAACAGAGG TGAGATTGCC ATCCGAGTGT TTCGTGCGTG CACAGAGCTG 
GGTATCCGCA CAGTGGCTGT CTACTCGGAG CAGGACACAG GGCAGATGCA CAGGCAGAAA 
GCTGATGAAG CCTACCTTAT TGGCCGTGGC CTGGCACCTG TGCAGGCCTA CCTGCACATT 
CCAGACATCA TCAAGGTGGC CAAGGAAAAT GGTGTAGATG CGGTGCATCC TGGCTATGGG 
TTCCTCTCAG AGCGAGCAGA CTTTGCCCAG GCCTGCCAAG ATGCTGGAGT CCGGTTCATT 
GGTCCAAGCC CAGAGGTGGT CCGCAAGATG GGAGACAAGG TGGAAGCCCG GGCCATTGCC 
ATCGCTGCAG GCGTTCCAGT GGTCCCCGGC ACGGACTCCC CCATCAGCTC CCTGCACGAG 
GCGCATGAGT TCTCCAACAC CTACGGCTTC CCTATTATCT TCAAGGCCGC CTACGGAGGT 
GGGGGCCGCG GCATGCGGGT CGTGCATAGC TATGAGGAGT TGGAAGAGAA TTACACCCGG 
GCCTACTCCG AGGCCTTGGC AGCCTTTGGG AATGGAGCGT TGTTTGTGGA GAAGTTCATT 
GAGAAGCCAA GGCACATTGA GGTGCAGATC CTAGGGGACC AGTATGGGAA CATCCTGCAC 
CTGTACGAGC GAGACTGCTC CATCCAGCGT CGGCACCAGA AGGTGGTAGA GATCGCCCCT 
GCTACCCACC TGGATCCCCA ACTTCGCTCA CGTCTCACCA GTGACTCTGT CAAACTTGCC 
AAGCAGGTAG GCTATGAGAA CGCCGGCACT GTGGAGTTCC TGGTGGACAA GCACGGCAAG 
CACTACTTCA TCGAGGTCAA TTCCCGCCTG CAGGTGGAGC ACACGGTCAC CGAGGAGATC 
ACAGATGTGG ACCTGGTCCA TGCTCAGATC CACGTGTCCG AAGGCCGGAG CCTGCCTGAC 
CTGGGCCTGC GGCAGGAGAA CATCCGCATC AATGGCTGTG CCATTCAGTG TCGGGTCACC 
ACCGAGGACC CTGCACGCAG CTTCCAGCCA GACACCGGCC GCATTGAGGT TTTCCGGAGT 
GGTGAGGGCA TGGGCATCCG CCTGGACAAC GCCTCTGCAT TCCAGGGCGC TGTCATATCG 
CCCCACTATG ACTCTCTGCT CGTCAAGGTC ATTGCACACG GCAAAGACCA CCCCACAGCT 
GCCACCAAGA TGAGCAGAGC CCTGGCCGAG TTCCGTGTCC GAGGTGTAAA GACCAACATC 
CCCTTCCTGC AGAATGTTCT CAACAACCAG CAGTTCCTGG CAGGCACAGT GGACACCCAG 
TTCATCGATG AGAACCCTGA GCTGTTCCAG CTTCGGCCTG CACAGAACCG GGCCCAGAAG 
TTGCTACATT ACCTCGGACA TGTCATGGTG AATGGCCCTA CCACTCCAAT CCCTGTCAAT 
GTGAGCCCCA GTCCTGTGGA TCCTGCTGTT CCTGTGGTGC CCATAGGCCC ACCTCCAGCT 
GGTTTCAGGG ACATCCTTCT GCGAGAAGGG CCAGAGGGCT TTGCCCGAGC TGTGCGGAAT 
CACCAGGGGC TGCTGTTGAT GGACACAACC TTCCGGGATG CCCACCAGTC ACTACTGGCC 
ACTAGAGTGC GCACACATGA TCTCAAAAAG ATTGCGCCCT ATGTTGCCCA CAACTTCAAC 
AAGCTCTTCA GCATGGAGAA CTGGGGAGGC GCCACGTTCG ACGTTGCCAT GCGCTTCCTG 
TACGAGTGCC CCTGGCGGCG GCTCCAGGAG CTCCGGGAGC TTATCCCGAA CATCCCGTTC 
CAGATGCTAC TGAGGGGGGC CAATGCTGTG GGCTACACCA ACTACCCTGA CAACGTGGTC 
TTCAAGTTCT GTGAGGTGGC CAAAGAGAAT GGTATGGACG TCTTCCGAGT CTTTGACTCC 
CTCAACTACT TGCCAAACAT GCTGCTGGGC ATGGAAGCAG CAGGCAGTGC TGGGGGTGTG 
GTGGAGGCTG CCATCTCATA CACGGGGGAC GTGGCTGACC CTAGTCGCAC TAAATACTCA 
CTGGAGTACT ACATGGGCTT AGCTGAAGAA CTGGTGCGAG CTGGCACTCA CATCCTGTGC 
ATTAAGGACA TGGCGGGCCT GCTGAAGCCT GCCGCCTGCA CCATGCTGGT CAGCTCCCTC 
CGGGACCGAT TCCCCGACCT CCCACTGCAC ATCCATACCC ATGATACATC AGGGGCAGGT 
GTGGCAGCCA TGCTGGCCTG TGCACAAGCA GGGGCTGATG TTGTGGACGT GGCAGTAGAC 
TCCATGTCTG GGATGACCTC ACAGCCAAGC ATGGGGGCCC TGGTGGCCTG TACCAAAGGG 
ACTCCTTTGG ACACAGAGGT ACCCCTGGAG CGTGTGTTTG ACTACAGTGA GTACTGGGAA 
GGGGCTCGGG GACTGTACGC AGCCTTCGAT TGCACGGCTA CCATGAAGTC TGGCAACTCC 
GACGTGTATG AGAATGAGAT TCCAGGGGGC CAGTACACCA ACCTGCACTT CCAGGCCCAT 
AGCATGGGGC TTGGCTCCAA GTTCAAGGAG GTCAAGAAGG CCTATGTGGA GGCTAACCAG 
ATGCTGGGGG ACCTCATCAA GGTGACACCA TCCTCCAAGA TTGTGGGGGA CCTGGCCCAG 
TTCATGGTGC AGAATGGGTT GAGCCGGGCA GAGGCAGAAG CTCAGGCAGA AGAGCTGTCC 
TTCCCCCGCT CTGTGGTGGA GTTCCTGCAG GGCTACATTG GCATTCCCCA TGGGGGTTTC 
CCTGAGCCCT TTCGCTCTAA GGTGCTAAAG GACCTGCCAA GAATAGAGGG GCGGCCTGGA 
GCCTCCCTCC CTCCCCTGAA CCTGAAGGAG CTGGAGAAGG ACCTGATTGA TAGGCATGGG 
GAGGAGGTGA CCCCAGAGGA CGTCCTCTCT GCAGCCATGT ACCCTGATGT CTTTGCTCAA 
TTCAAAGACT TCACGGCTAC CTTCGGCCCC CTGGATAGCC TTAATACTCG TCTCTTTCTT 
CAAGGACCCA AAATTGCAGA GGAGTTTGAG GTTGAGCTGG AACGGGGCAA GACCCTGCAC 
ATCAAAGCCC TGGCTGTAAG CGACCTGAAC CGTGCTGGCC AGAGGCAGGT GTTCTTTGAA 
CTCAATGGGC AGCTTCGATC CATTCTGGTT AAAGACACCC AGGCCATGAA GGAGATGCAC 
TTCCATCCCA AGGCTTTGAA GGATGTGAAG GGCCAAATTG GGGCCCCGAT GCCTGGGAAG 
GTCATAGACA TCAAGGTGGC AGCAGGGGAC AAGGTGGCTA AGGGCCAGCC CCTCTGTGTG 
CTCAGCGCCA TGAAGATGGA GACTGTGGTG ACTTCGCCCA TGGAGGGCAC TATCCGAAAG 
GTTCATGTTA CCAAGGACAT GACTCTGGAA GGCGACGACC TCATCCTAGA GATTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156418.1
CDS	145..3684
Translation	MMLKFQTVRGGLRLLGVRRSSSAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDSPISSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVNVSPSPVDPAVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGDKVAKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_001162946.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate carboxylase (Pcx), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162946.1

ORF Insert Sequence:

ATGATGCTGA AGTTCCAAAC AGTTCGAGGG GGCCTGAGGC TCCTGGGTGT CCGCCGATCC 
TCCTCGGCCC CTGTTGCCTC CCCAAATGTC CGGCGTCTGG AGTATAAGCC TATCAAGAAA 
GTAATGGTGG CCAACAGAGG TGAGATTGCC ATCCGAGTGT TTCGTGCGTG CACAGAGCTG 
GGTATCCGCA CAGTGGCTGT CTACTCGGAG CAGGACACAG GGCAGATGCA CAGGCAGAAA 
GCTGATGAAG CCTACCTTAT TGGCCGTGGC CTGGCACCTG TGCAGGCCTA CCTGCACATT 
CCAGACATCA TCAAGGTGGC CAAGGAAAAT GGTGTAGATG CGGTGCATCC TGGCTATGGG 
TTCCTCTCAG AGCGAGCAGA CTTTGCCCAG GCCTGCCAAG ATGCTGGAGT CCGGTTCATT 
GGTCCAAGCC CAGAGGTGGT CCGCAAGATG GGAGACAAGG TGGAAGCCCG GGCCATTGCC 
ATCGCTGCAG GCGTTCCAGT GGTCCCCGGC ACGGACTCCC CCATCAGCTC CCTGCACGAG 
GCGCATGAGT TCTCCAACAC CTACGGCTTC CCTATTATCT TCAAGGCCGC CTACGGAGGT 
GGGGGCCGCG GCATGCGGGT CGTGCATAGC TATGAGGAGT TGGAAGAGAA TTACACCCGG 
GCCTACTCCG AGGCCTTGGC AGCCTTTGGG AATGGAGCGT TGTTTGTGGA GAAGTTCATT 
GAGAAGCCAA GGCACATTGA GGTGCAGATC CTAGGGGACC AGTATGGGAA CATCCTGCAC 
CTGTACGAGC GAGACTGCTC CATCCAGCGT CGGCACCAGA AGGTGGTAGA GATCGCCCCT 
GCTACCCACC TGGATCCCCA ACTTCGCTCA CGTCTCACCA GTGACTCTGT CAAACTTGCC 
AAGCAGGTAG GCTATGAGAA CGCCGGCACT GTGGAGTTCC TGGTGGACAA GCACGGCAAG 
CACTACTTCA TCGAGGTCAA TTCCCGCCTG CAGGTGGAGC ACACGGTCAC CGAGGAGATC 
ACAGATGTGG ACCTGGTCCA TGCTCAGATC CACGTGTCCG AAGGCCGGAG CCTGCCTGAC 
CTGGGCCTGC GGCAGGAGAA CATCCGCATC AATGGCTGTG CCATTCAGTG TCGGGTCACC 
ACCGAGGACC CTGCACGCAG CTTCCAGCCA GACACCGGCC GCATTGAGGT TTTCCGGAGT 
GGTGAGGGCA TGGGCATCCG CCTGGACAAC GCCTCTGCAT TCCAGGGCGC TGTCATATCG 
CCCCACTATG ACTCTCTGCT CGTCAAGGTC ATTGCACACG GCAAAGACCA CCCCACAGCT 
GCCACCAAGA TGAGCAGAGC CCTGGCCGAG TTCCGTGTCC GAGGTGTAAA GACCAACATC 
CCCTTCCTGC AGAATGTTCT CAACAACCAG CAGTTCCTGG CAGGCACAGT GGACACCCAG 
TTCATCGATG AGAACCCTGA GCTGTTCCAG CTTCGGCCTG CACAGAACCG GGCCCAGAAG 
TTGCTACATT ACCTCGGACA TGTCATGGTG AATGGCCCTA CCACTCCAAT CCCTGTCAAT 
GTGAGCCCCA GTCCTGTGGA TCCTGCTGTT CCTGTGGTGC CCATAGGCCC ACCTCCAGCT 
GGTTTCAGGG ACATCCTTCT GCGAGAAGGG CCAGAGGGCT TTGCCCGAGC TGTGCGGAAT 
CACCAGGGGC TGCTGTTGAT GGACACAACC TTCCGGGATG CCCACCAGTC ACTACTGGCC 
ACTAGAGTGC GCACACATGA TCTCAAAAAG ATTGCGCCCT ATGTTGCCCA CAACTTCAAC 
AAGCTCTTCA GCATGGAGAA CTGGGGAGGC GCCACGTTCG ACGTTGCCAT GCGCTTCCTG 
TACGAGTGCC CCTGGCGGCG GCTCCAGGAG CTCCGGGAGC TTATCCCGAA CATCCCGTTC 
CAGATGCTAC TGAGGGGGGC CAATGCTGTG GGCTACACCA ACTACCCTGA CAACGTGGTC 
TTCAAGTTCT GTGAGGTGGC CAAAGAGAAT GGTATGGACG TCTTCCGAGT CTTTGACTCC 
CTCAACTACT TGCCAAACAT GCTGCTGGGC ATGGAAGCAG CAGGCAGTGC TGGGGGTGTG 
GTGGAGGCTG CCATCTCATA CACGGGGGAC GTGGCTGACC CTAGTCGCAC TAAATACTCA 
CTGGAGTACT ACATGGGCTT AGCTGAAGAA CTGGTGCGAG CTGGCACTCA CATCCTGTGC 
ATTAAGGACA TGGCGGGCCT GCTGAAGCCT GCCGCCTGCA CCATGCTGGT CAGCTCCCTC 
CGGGACCGAT TCCCCGACCT CCCACTGCAC ATCCATACCC ATGATACATC AGGGGCAGGT 
GTGGCAGCCA TGCTGGCCTG TGCACAAGCA GGGGCTGATG TTGTGGACGT GGCAGTAGAC 
TCCATGTCTG GGATGACCTC ACAGCCAAGC ATGGGGGCCC TGGTGGCCTG TACCAAAGGG 
ACTCCTTTGG ACACAGAGGT ACCCCTGGAG CGTGTGTTTG ACTACAGTGA GTACTGGGAA 
GGGGCTCGGG GACTGTACGC AGCCTTCGAT TGCACGGCTA CCATGAAGTC TGGCAACTCC 
GACGTGTATG AGAATGAGAT TCCAGGGGGC CAGTACACCA ACCTGCACTT CCAGGCCCAT 
AGCATGGGGC TTGGCTCCAA GTTCAAGGAG GTCAAGAAGG CCTATGTGGA GGCTAACCAG 
ATGCTGGGGG ACCTCATCAA GGTGACACCA TCCTCCAAGA TTGTGGGGGA CCTGGCCCAG 
TTCATGGTGC AGAATGGGTT GAGCCGGGCA GAGGCAGAAG CTCAGGCAGA AGAGCTGTCC 
TTCCCCCGCT CTGTGGTGGA GTTCCTGCAG GGCTACATTG GCATTCCCCA TGGGGGTTTC 
CCTGAGCCCT TTCGCTCTAA GGTGCTAAAG GACCTGCCAA GAATAGAGGG GCGGCCTGGA 
GCCTCCCTCC CTCCCCTGAA CCTGAAGGAG CTGGAGAAGG ACCTGATTGA TAGGCATGGG 
GAGGAGGTGA CCCCAGAGGA CGTCCTCTCT GCAGCCATGT ACCCTGATGT CTTTGCTCAA 
TTCAAAGACT TCACGGCTAC CTTCGGCCCC CTGGATAGCC TTAATACTCG TCTCTTTCTT 
CAAGGACCCA AAATTGCAGA GGAGTTTGAG GTTGAGCTGG AACGGGGCAA GACCCTGCAC 
ATCAAAGCCC TGGCTGTAAG CGACCTGAAC CGTGCTGGCC AGAGGCAGGT GTTCTTTGAA 
CTCAATGGGC AGCTTCGATC CATTCTGGTT AAAGACACCC AGGCCATGAA GGAGATGCAC 
TTCCATCCCA AGGCTTTGAA GGATGTGAAG GGCCAAATTG GGGCCCCGAT GCCTGGGAAG 
GTCATAGACA TCAAGGTGGC AGCAGGGGAC AAGGTGGCTA AGGGCCAGCC CCTCTGTGTG 
CTCAGCGCCA TGAAGATGGA GACTGTGGTG ACTTCGCCCA TGGAGGGCAC TATCCGAAAG 
GTTCATGTTA CCAAGGACAT GACTCTGGAA GGCGACGACC TCATCCTAGA GATTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcx ( NM_008797.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032823.2 Mus musculus pyruvate carboxylase (Pcx), transcript variant 2, mRNA.

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CloneID	OMu15330
Clone ID Related Accession (Same CDS sequence)	NM_008797.3 , XM_006531678.3 , XM_006531678.4
Accession Version	NM_008797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	pyruvate carboxylase, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY059816.1, BY139237.1, BG087228.2, AK148328.1, AK077665.1 and AA619324.1. On Jul 8, 2009 this sequence version replaced NM_008797.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L09192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
TCGGCCCCTG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ATAAGCCTAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCGTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACAG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGCCTG GCACCTGTGC AGGCCTACCT GCACATTCCA 
GACATCATCA AGGTGGCCAA GGAAAATGGT GTAGATGCGG TGCATCCTGG CTATGGGTTC 
CTCTCAGAGC GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG GTTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATC 
GCTGCAGGCG TTCCAGTGGT CCCCGGCACG GACTCCCCCA TCAGCTCCCT GCACGAGGCG 
CATGAGTTCT CCAACACCTA CGGCTTCCCT ATTATCTTCA AGGCCGCCTA CGGAGGTGGG 
GGCCGCGGCA TGCGGGTCGT GCATAGCTAT GAGGAGTTGG AAGAGAATTA CACCCGGGCC 
TACTCCGAGG CCTTGGCAGC CTTTGGGAAT GGAGCGTTGT TTGTGGAGAA GTTCATTGAG 
AAGCCAAGGC ACATTGAGGT GCAGATCCTA GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGTCGG CACCAGAAGG TGGTAGAGAT CGCCCCTGCT 
ACCCACCTGG ATCCCCAACT TCGCTCACGT CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTAGGCT ATGAGAACGC CGGCACTGTG GAGTTCCTGG TGGACAAGCA CGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACCGA GGAGATCACA 
GATGTGGACC TGGTCCATGC TCAGATCCAC GTGTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAT GGCTGTGCCA TTCAGTGTCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACCGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCATTCC AGGGCGCTGT CATATCGCCC 
CACTATGACT CTCTGCTCGT CAAGGTCATT GCACACGGCA AAGACCACCC CACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTGGCAG GCACAGTGGA CACCCAGTTC 
ATCGATGAGA ACCCTGAGCT GTTCCAGCTT CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TCGGACATGT CATGGTGAAT GGCCCTACCA CTCCAATCCC TGTCAATGTG 
AGCCCCAGTC CTGTGGATCC TGCTGTTCCT GTGGTGCCCA TAGGCCCACC TCCAGCTGGT 
TTCAGGGACA TCCTTCTGCG AGAAGGGCCA GAGGGCTTTG CCCGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGTTGATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTGGCCACT 
AGAGTGCGCA CACATGATCT CAAAAAGATT GCGCCCTATG TTGCCCACAA CTTCAACAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGCGCC ACGTTCGACG TTGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTTA TCCCGAACAT CCCGTTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGT ATGGACGTCT TCCGAGTCTT TGACTCCCTC 
AACTACTTGC CAAACATGCT GCTGGGCATG GAAGCAGCAG GCAGTGCTGG GGGTGTGGTG 
GAGGCTGCCA TCTCATACAC GGGGGACGTG GCTGACCCTA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCTG GCACTCACAT CCTGTGCATT 
AAGGACATGG CGGGCCTGCT GAAGCCTGCC GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGATTCC CCGACCTCCC ACTGCACATC CATACCCATG ATACATCAGG GGCAGGTGTG 
GCAGCCATGC TGGCCTGTGC ACAAGCAGGG GCTGATGTTG TGGACGTGGC AGTAGACTCC 
ATGTCTGGGA TGACCTCACA GCCAAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTTTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA CTGGGAAGGG 
GCTCGGGGAC TGTACGCAGC CTTCGATTGC ACGGCTACCA TGAAGTCTGG CAACTCCGAC 
GTGTATGAGA ATGAGATTCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCATAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ATGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAGCCCTTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGGGAG 
GAGGTGACCC CAGAGGACGT CCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAATTC 
AAAGACTTCA CGGCTACCTT CGGCCCCCTG GATAGCCTTA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CCTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CTTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCGATGCC TGGGAAGGTC 
ATAGACATCA AGGTGGCAGC AGGGGACAAG GTGGCTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CATGTTACCA AGGACATGAC TCTGGAAGGC GACGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032823.2
CDS	125..3661
Translation	MLKFQTVRGGLRLLGVRRSSSAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDSPISSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVNVSPSPVDPAVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGDKVAKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_008797.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate carboxylase (Pcx), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008797.3

ORF Insert Sequence:

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
TCGGCCCCTG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ATAAGCCTAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCGTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACAG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGCCTG GCACCTGTGC AGGCCTACCT GCACATTCCA 
GACATCATCA AGGTGGCCAA GGAAAATGGT GTAGATGCGG TGCATCCTGG CTATGGGTTC 
CTCTCAGAGC GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG GTTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATC 
GCTGCAGGCG TTCCAGTGGT CCCCGGCACG GACTCCCCCA TCAGCTCCCT GCACGAGGCG 
CATGAGTTCT CCAACACCTA CGGCTTCCCT ATTATCTTCA AGGCCGCCTA CGGAGGTGGG 
GGCCGCGGCA TGCGGGTCGT GCATAGCTAT GAGGAGTTGG AAGAGAATTA CACCCGGGCC 
TACTCCGAGG CCTTGGCAGC CTTTGGGAAT GGAGCGTTGT TTGTGGAGAA GTTCATTGAG 
AAGCCAAGGC ACATTGAGGT GCAGATCCTA GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGTCGG CACCAGAAGG TGGTAGAGAT CGCCCCTGCT 
ACCCACCTGG ATCCCCAACT TCGCTCACGT CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTAGGCT ATGAGAACGC CGGCACTGTG GAGTTCCTGG TGGACAAGCA CGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACCGA GGAGATCACA 
GATGTGGACC TGGTCCATGC TCAGATCCAC GTGTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAT GGCTGTGCCA TTCAGTGTCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACCGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCATTCC AGGGCGCTGT CATATCGCCC 
CACTATGACT CTCTGCTCGT CAAGGTCATT GCACACGGCA AAGACCACCC CACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTGGCAG GCACAGTGGA CACCCAGTTC 
ATCGATGAGA ACCCTGAGCT GTTCCAGCTT CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TCGGACATGT CATGGTGAAT GGCCCTACCA CTCCAATCCC TGTCAATGTG 
AGCCCCAGTC CTGTGGATCC TGCTGTTCCT GTGGTGCCCA TAGGCCCACC TCCAGCTGGT 
TTCAGGGACA TCCTTCTGCG AGAAGGGCCA GAGGGCTTTG CCCGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGTTGATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTGGCCACT 
AGAGTGCGCA CACATGATCT CAAAAAGATT GCGCCCTATG TTGCCCACAA CTTCAACAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGCGCC ACGTTCGACG TTGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTTA TCCCGAACAT CCCGTTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGT ATGGACGTCT TCCGAGTCTT TGACTCCCTC 
AACTACTTGC CAAACATGCT GCTGGGCATG GAAGCAGCAG GCAGTGCTGG GGGTGTGGTG 
GAGGCTGCCA TCTCATACAC GGGGGACGTG GCTGACCCTA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCTG GCACTCACAT CCTGTGCATT 
AAGGACATGG CGGGCCTGCT GAAGCCTGCC GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGATTCC CCGACCTCCC ACTGCACATC CATACCCATG ATACATCAGG GGCAGGTGTG 
GCAGCCATGC TGGCCTGTGC ACAAGCAGGG GCTGATGTTG TGGACGTGGC AGTAGACTCC 
ATGTCTGGGA TGACCTCACA GCCAAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTTTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA CTGGGAAGGG 
GCTCGGGGAC TGTACGCAGC CTTCGATTGC ACGGCTACCA TGAAGTCTGG CAACTCCGAC 
GTGTATGAGA ATGAGATTCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCATAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ATGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAGCCCTTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGGGAG 
GAGGTGACCC CAGAGGACGT CCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAATTC 
AAAGACTTCA CGGCTACCTT CGGCCCCCTG GATAGCCTTA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CCTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CTTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCGATGCC TGGGAAGGTC 
ATAGACATCA AGGTGGCAGC AGGGGACAAG GTGGCTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CATGTTACCA AGGACATGAC TCTGGAAGGC GACGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcx ( XM_006531678.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531741.1 Mus musculus pyruvate carboxylase (Pcx), transcript variant X1, mRNA.

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CloneID	OMu15330
Clone ID Related Accession (Same CDS sequence)	NM_008797.3 , XM_006531678.3 , XM_006531678.4
Accession Version	XM_006531678.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
TCGGCCCCTG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ATAAGCCTAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCGTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACAG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGCCTG GCACCTGTGC AGGCCTACCT GCACATTCCA 
GACATCATCA AGGTGGCCAA GGAAAATGGT GTAGATGCGG TGCATCCTGG CTATGGGTTC 
CTCTCAGAGC GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG GTTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATC 
GCTGCAGGCG TTCCAGTGGT CCCCGGCACG GACTCCCCCA TCAGCTCCCT GCACGAGGCG 
CATGAGTTCT CCAACACCTA CGGCTTCCCT ATTATCTTCA AGGCCGCCTA CGGAGGTGGG 
GGCCGCGGCA TGCGGGTCGT GCATAGCTAT GAGGAGTTGG AAGAGAATTA CACCCGGGCC 
TACTCCGAGG CCTTGGCAGC CTTTGGGAAT GGAGCGTTGT TTGTGGAGAA GTTCATTGAG 
AAGCCAAGGC ACATTGAGGT GCAGATCCTA GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGTCGG CACCAGAAGG TGGTAGAGAT CGCCCCTGCT 
ACCCACCTGG ATCCCCAACT TCGCTCACGT CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTAGGCT ATGAGAACGC CGGCACTGTG GAGTTCCTGG TGGACAAGCA CGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACCGA GGAGATCACA 
GATGTGGACC TGGTCCATGC TCAGATCCAC GTGTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAT GGCTGTGCCA TTCAGTGTCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACCGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCATTCC AGGGCGCTGT CATATCGCCC 
CACTATGACT CTCTGCTCGT CAAGGTCATT GCACACGGCA AAGACCACCC CACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTGGCAG GCACAGTGGA CACCCAGTTC 
ATCGATGAGA ACCCTGAGCT GTTCCAGCTT CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TCGGACATGT CATGGTGAAT GGCCCTACCA CTCCAATCCC TGTCAATGTG 
AGCCCCAGTC CTGTGGATCC TGCTGTTCCT GTGGTGCCCA TAGGCCCACC TCCAGCTGGT 
TTCAGGGACA TCCTTCTGCG AGAAGGGCCA GAGGGCTTTG CCCGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGTTGATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTGGCCACT 
AGAGTGCGCA CACATGATCT CAAAAAGATT GCGCCCTATG TTGCCCACAA CTTCAACAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGCGCC ACGTTCGACG TTGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTTA TCCCGAACAT CCCGTTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGT ATGGACGTCT TCCGAGTCTT TGACTCCCTC 
AACTACTTGC CAAACATGCT GCTGGGCATG GAAGCAGCAG GCAGTGCTGG GGGTGTGGTG 
GAGGCTGCCA TCTCATACAC GGGGGACGTG GCTGACCCTA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCTG GCACTCACAT CCTGTGCATT 
AAGGACATGG CGGGCCTGCT GAAGCCTGCC GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGATTCC CCGACCTCCC ACTGCACATC CATACCCATG ATACATCAGG GGCAGGTGTG 
GCAGCCATGC TGGCCTGTGC ACAAGCAGGG GCTGATGTTG TGGACGTGGC AGTAGACTCC 
ATGTCTGGGA TGACCTCACA GCCAAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTTTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA CTGGGAAGGG 
GCTCGGGGAC TGTACGCAGC CTTCGATTGC ACGGCTACCA TGAAGTCTGG CAACTCCGAC 
GTGTATGAGA ATGAGATTCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCATAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ATGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAGCCCTTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGGGAG 
GAGGTGACCC CAGAGGACGT CCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAATTC 
AAAGACTTCA CGGCTACCTT CGGCCCCCTG GATAGCCTTA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CCTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CTTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCGATGCC TGGGAAGGTC 
ATAGACATCA AGGTGGCAGC AGGGGACAAG GTGGCTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CATGTTACCA AGGACATGAC TCTGGAAGGC GACGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531741.1
CDS	160..3696
Translation	MLKFQTVRGGLRLLGVRRSSSAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDSPISSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVNVSPSPVDPAVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGDKVAKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006531678.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate carboxylase (Pcx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531678.3

ORF Insert Sequence:

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
TCGGCCCCTG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ATAAGCCTAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCGTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACAG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGCCTG GCACCTGTGC AGGCCTACCT GCACATTCCA 
GACATCATCA AGGTGGCCAA GGAAAATGGT GTAGATGCGG TGCATCCTGG CTATGGGTTC 
CTCTCAGAGC GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG GTTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATC 
GCTGCAGGCG TTCCAGTGGT CCCCGGCACG GACTCCCCCA TCAGCTCCCT GCACGAGGCG 
CATGAGTTCT CCAACACCTA CGGCTTCCCT ATTATCTTCA AGGCCGCCTA CGGAGGTGGG 
GGCCGCGGCA TGCGGGTCGT GCATAGCTAT GAGGAGTTGG AAGAGAATTA CACCCGGGCC 
TACTCCGAGG CCTTGGCAGC CTTTGGGAAT GGAGCGTTGT TTGTGGAGAA GTTCATTGAG 
AAGCCAAGGC ACATTGAGGT GCAGATCCTA GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGTCGG CACCAGAAGG TGGTAGAGAT CGCCCCTGCT 
ACCCACCTGG ATCCCCAACT TCGCTCACGT CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTAGGCT ATGAGAACGC CGGCACTGTG GAGTTCCTGG TGGACAAGCA CGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACCGA GGAGATCACA 
GATGTGGACC TGGTCCATGC TCAGATCCAC GTGTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAT GGCTGTGCCA TTCAGTGTCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACCGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAACGCC TCTGCATTCC AGGGCGCTGT CATATCGCCC 
CACTATGACT CTCTGCTCGT CAAGGTCATT GCACACGGCA AAGACCACCC CACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCCGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTGGCAG GCACAGTGGA CACCCAGTTC 
ATCGATGAGA ACCCTGAGCT GTTCCAGCTT CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TCGGACATGT CATGGTGAAT GGCCCTACCA CTCCAATCCC TGTCAATGTG 
AGCCCCAGTC CTGTGGATCC TGCTGTTCCT GTGGTGCCCA TAGGCCCACC TCCAGCTGGT 
TTCAGGGACA TCCTTCTGCG AGAAGGGCCA GAGGGCTTTG CCCGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGTTGATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTGGCCACT 
AGAGTGCGCA CACATGATCT CAAAAAGATT GCGCCCTATG TTGCCCACAA CTTCAACAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGCGCC ACGTTCGACG TTGCCATGCG CTTCCTGTAC 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTTA TCCCGAACAT CCCGTTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGT ATGGACGTCT TCCGAGTCTT TGACTCCCTC 
AACTACTTGC CAAACATGCT GCTGGGCATG GAAGCAGCAG GCAGTGCTGG GGGTGTGGTG 
GAGGCTGCCA TCTCATACAC GGGGGACGTG GCTGACCCTA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCTG GCACTCACAT CCTGTGCATT 
AAGGACATGG CGGGCCTGCT GAAGCCTGCC GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGATTCC CCGACCTCCC ACTGCACATC CATACCCATG ATACATCAGG GGCAGGTGTG 
GCAGCCATGC TGGCCTGTGC ACAAGCAGGG GCTGATGTTG TGGACGTGGC AGTAGACTCC 
ATGTCTGGGA TGACCTCACA GCCAAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTTTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA CTGGGAAGGG 
GCTCGGGGAC TGTACGCAGC CTTCGATTGC ACGGCTACCA TGAAGTCTGG CAACTCCGAC 
GTGTATGAGA ATGAGATTCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCATAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGACCT GGCCCAGTTC 
ATGGTGCAGA ATGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAGCCCTTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGGGAG 
GAGGTGACCC CAGAGGACGT CCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAATTC 
AAAGACTTCA CGGCTACCTT CGGCCCCCTG GATAGCCTTA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CCTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CTTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCGATGCC TGGGAAGGTC 
ATAGACATCA AGGTGGCAGC AGGGGACAAG GTGGCTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CATGTTACCA AGGACATGAC TCTGGAAGGC GACGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro.
Science (New York, N.Y.)356(6334)(2017 04)
Harrison SE,Sozen B,Christodoulou N,Kyprianou C,Zernicka-Goetz M

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
The Journal of cell biology216(3)675-693(2017 Mar)
Schatton D,Pla-Martin D,Marx MC,Hansen H,Mourier A,Nemazanyy I,Pessia A,Zentis P,Corona T,Kondylis V,Barth E,Schauss AC,Velagapudi V,Rugarli EI

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Reduction of islet pyruvate carboxylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice.
The Journal of endocrinology204(2)143-52(2010 Feb)
Han J,Liu YQ

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Pcx cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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