Pck1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pck1
Entrez Gene ID	18534
Full Name	phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms	AI265463,PEPCK,Pck-1
General protein information	Preferred Names phosphoenolpyruvate carboxykinase 1, cytosolic Names phosphoenolpyruvate carboxykinase, cytosolic [GTP] PEPCK-C phosphoenolpyruvate carboxykinase, cytosolic phosphoenolpyruvate carboxylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H3\|2 95.79 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011044.2	NP_035174.1	phosphoenolpyruvate carboxykinase, cytosolic [GTP]
NM_011044.3	NP_035174.1	phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PCK1	NP_777162.1
	PCK1	XP_001086710.1
Mus musculus (house mouse)	Pck1	NP_035174.1
Danio rerio (zebrafish)	pck1	NP_999916.1
Xenopus tropicalis (tropical clawed frog)	pck1	NP_001073036.1
Rattus norvegicus (Norway rat)	Pck1	NP_942075.1
Gallus gallus (chicken)	PCK1	NP_990802.1
Homo sapiens (human)	PCK1	NP_002582.3
Pan troglodytes (chimpanzee)	PCK1	XP_514745.2
Canis lupus familiaris (dog)	PCK1	NP_001184072.1
Drosophila melanogaster (fruit fly)	CG10924	NP_611341.1

Gene Ontology
Bibliography

Related articles in PubMed

Macrophages with a deletion of the
Ko CW, Counihan D, Wu J, Hatzoglou M, Puchowicz MA, Croniger CM
The Journal of biological chemistry(2018 Jan)

A Pck1-directed glycogen metabolic program regulates formation and maintenance of memory CD8
Ma R, Ji T, Zhang H, Dong W, Chen X, Xu P, Chen D, Liang X, Yin X, Liu Y, Ma J, Tang K, Zhang Y, Peng Y, Lu J, Zhang Y, Qin X, Cao X, Wan Y, Huang B
Nature cell biology20(1)21-27(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data (including data from studies in knockout mice) suggest that Pck1 in liver plays role in glucose homeostasis; here, Pck1 deficiency (in knockout mice) can be successfully treated by re-expression of Pck1 in liver, thus partially rescuing pups from fatal neonatal hypoglycemia. Pck1 appears to play roles in metabolism in lung and skeletal muscle.
Title: PEPCK-C reexpression in the liver counters neonatal hypoglycemia in Pck1

Silencing PCK1 or inhibiting its enzymatic activity slows the growth of tumor-repopulating cells in vitro and impedes tumorigenesis in vivo.
Title: Upregulation of cytosolic phosphoenolpyruvate carboxykinase is a critical metabolic event in melanoma cells that repopulate tumors.

PEPCK-M expression partially rescued defects in lipid metabolism, gluconeogenesis and TCA cycle function impaired by PEPCK-C deletion, while approximately 10% re-expression of PEPCK-C normalized most parameters.
Title: PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis.

FAT/CD36 regulates PEPCK in adipose tissue and that this could be secondary to reductions in lipolysis.
Title: FAT/CD36 regulates PEPCK expression in adipose tissue.

the role of IL-6 signalling in adipose tissue during exercise
Title: IL-6 indirectly modulates the induction of glyceroneogenic enzymes in adipose tissue during exercise.

The following Pck1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pck1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07357	NM_011044.3 Latest version!	Mus musculus phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$370.30 $529.00
View Old Accession Versions >>
OMu07357	NM_011044.2	Mus musculus phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$370.30 $529.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pck1 ( NM_011044.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035174.1 Mus musculus phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), mRNA.

Price & Availability↑

CloneID	OMu07357
Clone ID Related Accession (Same CDS sequence)	NM_011044.2 , NM_011044.3
Accession Version	NM_011044.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-30
Organism	Mus musculus(house mouse)
Product	phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL837509.11. On Nov 17, 2006 this sequence version replaced NM_011044.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028046.1, AK133496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCCTC AGCTGCATAA CGGTCTGGAC TTCTCTGCCA AGGTCATCCA GGGCAGCCTC 
GACAGCCTGC CCCAGGCAGT GAGGAAGTTC GTGGAAGGCA ATGCTCAGCT GTGCCAGCCG 
GAGTATATCC ACATCTGCGA TGGCTCCGAG GAGGAGTACG GGCAGTTGCT GGCCCACATG 
CAGGAGGAGG GTGTCATCCG CAAGCTGAAG AAATATGACA ACTGTTGGCT GGCTCTCACT 
GACCCTCGAG ATGTGGCCAG GATCGAAAGC AAGACAGTCA TCATCACCCA AGAGCAGAGA 
GACACAGTGC CCATCCCCAA AACTGGCCTC AGCCAGCTGG GCCGCTGGAT GTCGGAAGAG 
GACTTTGAGA AAGCATTCAA CGCCAGGTTC CCAGGGTGCA TGAAAGGCCG CACCATGTAT 
GTCATCCCAT TCAGCATGGG GCCACTGGGC TCGCCGCTGG CCAAGATTGG TATTGAACTG 
ACAGACTCGC CCTATGTGGT GGCCAGCATG CGGATCATGA CTCGGATGGG CATATCTGTG 
CTGGAGGCCC TGGGAGATGG GGAGTTCATC AAGTGCCTGC ACTCTGTGGG GTGCCCTCTC 
CCCTTAAAAA AGCCTTTGGT CAACAACTGG GCCTGCAACC CTGAGCTGAC CCTGATCGCC 
CACCTCCCGG ACCGCAGAGA GATCATCTCC TTTGGAAGCG GATATGGTGG GAACTCACTA 
CTCGGGAAGA AATGCTTTGC GTTGCGGATC GCCAGCCGTC TGGCTAAGGA GGAAGGGTGG 
CTGGCGGAGC ATATGCTGAT CCTGGGCATA ACTAACCCCG AAGGCAAGAA GAAATACCTG 
GCCGCAGCCT TCCCTAGTGC CTGTGGGAAG ACTAACTTGG CCATGATGAA CCCCAGCCTG 
CCCGGGTGGA AGGTCGAATG TGTGGGCGAT GACATTGCCT GGATGAAGTT TGATGCCCAA 
GGCAACTTAA GGGCTATCAA CCCAGAAAAC GGGTTTTTTG GAGTTGCTCC TGGCACCTCA 
GTGAAGACAA ATCCAAATGC CATTAAAACC ATCCAGAAAA ACACCATCTT CACCAACGTG 
GCCGAGACTA GCGATGGGGG TGTTTACTGG GAAGGCATCG ATGAGCCGCT GGCCCCGGGA 
GTCACCATCA CCTCCTGGAA GAACAAGGAG TGGAGACCGC AGGACGCGGA ACCATGTGCC 
CATCCCAACT CGAGATTCTG CACCCCTGCC AGCCAGTGCC CCATTATTGA CCCTGCCTGG 
GAATCTCCAG AAGGAGTACC CATTGAGGGT ATCATCTTTG GTGGCCGTAG ACCTGAAGGT 
GTCCCCCTTG TCTATGAAGC CCTCAGCTGG CAGCATGGGG TGTTTGTAGG AGCAGCCATG 
AGATCTGAGG CCACAGCTGC TGCAGAACAC AAGGGCAAGA TCATCATGCA CGACCCCTTT 
GCCATGCGAC CCTTCTTCGG CTACAACTTC GGCAAATACC TGGCCCACTG GCTGAGCATG 
GCCCACCGCC CAGCAGCCAA GTTGCCCAAG ATCTTCCATG TCAACTGGTT CCGGAAGGAC 
AAAGATGGCA AGTTCCTCTG GCCAGGCTTT GGCGAGAACT CCCGGGTGCT GGAGTGGATG 
TTCGGGCGGA TTGAAGGGGA AGACAGCGCC AAGCTCACGC CCATCGGCTA CATCCCTAAG 
GAAAACGCCT TGAACCTGAA AGGCCTGGGG GGCGTCAACG TGGAGGAGCT GTTTGGGATC 
TCTAAGGAGT TCTGGGAGAA GGAGGTGGAG GAGATCGACA GGTATCTGGA GGACCAGGTC 
AACACCGACC TCCCTTACGA AATTGAGAGG GAGCTCCGAG CCCTGAAACA GAGAATCAGC 
CAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035174.1
CDS	141..2009
Translation	MPPQLHNGLDFSAKVIQGSLDSLPQAVRKFVEGNAQLCQPEYIHICDGSEEEYGQLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGISVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDAEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPEGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKDGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYIPKENALNLKGLGGVNVEELFGISKEFWEKEVEEIDRYLEDQVNTDLPYEIERELRALKQRISQM

Target ORF information:

RefSeq Version	NM_011044.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011044.2

ORF Insert Sequence:

ATGCCTCCTC AGCTGCATAA CGGTCTGGAC TTCTCTGCCA AGGTCATCCA GGGCAGCCTC 
GACAGCCTGC CCCAGGCAGT GAGGAAGTTC GTGGAAGGCA ATGCTCAGCT GTGCCAGCCG 
GAGTATATCC ACATCTGCGA TGGCTCCGAG GAGGAGTACG GGCAGTTGCT GGCCCACATG 
CAGGAGGAGG GTGTCATCCG CAAGCTGAAG AAATATGACA ACTGTTGGCT GGCTCTCACT 
GACCCTCGAG ATGTGGCCAG GATCGAAAGC AAGACAGTCA TCATCACCCA AGAGCAGAGA 
GACACAGTGC CCATCCCCAA AACTGGCCTC AGCCAGCTGG GCCGCTGGAT GTCGGAAGAG 
GACTTTGAGA AAGCATTCAA CGCCAGGTTC CCAGGGTGCA TGAAAGGCCG CACCATGTAT 
GTCATCCCAT TCAGCATGGG GCCACTGGGC TCGCCGCTGG CCAAGATTGG TATTGAACTG 
ACAGACTCGC CCTATGTGGT GGCCAGCATG CGGATCATGA CTCGGATGGG CATATCTGTG 
CTGGAGGCCC TGGGAGATGG GGAGTTCATC AAGTGCCTGC ACTCTGTGGG GTGCCCTCTC 
CCCTTAAAAA AGCCTTTGGT CAACAACTGG GCCTGCAACC CTGAGCTGAC CCTGATCGCC 
CACCTCCCGG ACCGCAGAGA GATCATCTCC TTTGGAAGCG GATATGGTGG GAACTCACTA 
CTCGGGAAGA AATGCTTTGC GTTGCGGATC GCCAGCCGTC TGGCTAAGGA GGAAGGGTGG 
CTGGCGGAGC ATATGCTGAT CCTGGGCATA ACTAACCCCG AAGGCAAGAA GAAATACCTG 
GCCGCAGCCT TCCCTAGTGC CTGTGGGAAG ACTAACTTGG CCATGATGAA CCCCAGCCTG 
CCCGGGTGGA AGGTCGAATG TGTGGGCGAT GACATTGCCT GGATGAAGTT TGATGCCCAA 
GGCAACTTAA GGGCTATCAA CCCAGAAAAC GGGTTTTTTG GAGTTGCTCC TGGCACCTCA 
GTGAAGACAA ATCCAAATGC CATTAAAACC ATCCAGAAAA ACACCATCTT CACCAACGTG 
GCCGAGACTA GCGATGGGGG TGTTTACTGG GAAGGCATCG ATGAGCCGCT GGCCCCGGGA 
GTCACCATCA CCTCCTGGAA GAACAAGGAG TGGAGACCGC AGGACGCGGA ACCATGTGCC 
CATCCCAACT CGAGATTCTG CACCCCTGCC AGCCAGTGCC CCATTATTGA CCCTGCCTGG 
GAATCTCCAG AAGGAGTACC CATTGAGGGT ATCATCTTTG GTGGCCGTAG ACCTGAAGGT 
GTCCCCCTTG TCTATGAAGC CCTCAGCTGG CAGCATGGGG TGTTTGTAGG AGCAGCCATG 
AGATCTGAGG CCACAGCTGC TGCAGAACAC AAGGGCAAGA TCATCATGCA CGACCCCTTT 
GCCATGCGAC CCTTCTTCGG CTACAACTTC GGCAAATACC TGGCCCACTG GCTGAGCATG 
GCCCACCGCC CAGCAGCCAA GTTGCCCAAG ATCTTCCATG TCAACTGGTT CCGGAAGGAC 
AAAGATGGCA AGTTCCTCTG GCCAGGCTTT GGCGAGAACT CCCGGGTGCT GGAGTGGATG 
TTCGGGCGGA TTGAAGGGGA AGACAGCGCC AAGCTCACGC CCATCGGCTA CATCCCTAAG 
GAAAACGCCT TGAACCTGAA AGGCCTGGGG GGCGTCAACG TGGAGGAGCT GTTTGGGATC 
TCTAAGGAGT TCTGGGAGAA GGAGGTGGAG GAGATCGACA GGTATCTGGA GGACCAGGTC 
AACACCGACC TCCCTTACGA AATTGAGAGG GAGCTCCGAG CCCTGAAACA GAGAATCAGC 
CAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pck1 ( NM_011044.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035174.1 Mus musculus phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), mRNA.

Price & Availability↑

CloneID	OMu07357
Clone ID Related Accession (Same CDS sequence)	NM_011044.2 , NM_011044.3
Accession Version	NM_011044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL837509.11. On Apr 24, 2018 this sequence version replaced NM_011044.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028046.1, AK149525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCCTC AGCTGCATAA CGGTCTGGAC TTCTCTGCCA AGGTCATCCA GGGCAGCCTC 
GACAGCCTGC CCCAGGCAGT GAGGAAGTTC GTGGAAGGCA ATGCTCAGCT GTGCCAGCCG 
GAGTATATCC ACATCTGCGA TGGCTCCGAG GAGGAGTACG GGCAGTTGCT GGCCCACATG 
CAGGAGGAGG GTGTCATCCG CAAGCTGAAG AAATATGACA ACTGTTGGCT GGCTCTCACT 
GACCCTCGAG ATGTGGCCAG GATCGAAAGC AAGACAGTCA TCATCACCCA AGAGCAGAGA 
GACACAGTGC CCATCCCCAA AACTGGCCTC AGCCAGCTGG GCCGCTGGAT GTCGGAAGAG 
GACTTTGAGA AAGCATTCAA CGCCAGGTTC CCAGGGTGCA TGAAAGGCCG CACCATGTAT 
GTCATCCCAT TCAGCATGGG GCCACTGGGC TCGCCGCTGG CCAAGATTGG TATTGAACTG 
ACAGACTCGC CCTATGTGGT GGCCAGCATG CGGATCATGA CTCGGATGGG CATATCTGTG 
CTGGAGGCCC TGGGAGATGG GGAGTTCATC AAGTGCCTGC ACTCTGTGGG GTGCCCTCTC 
CCCTTAAAAA AGCCTTTGGT CAACAACTGG GCCTGCAACC CTGAGCTGAC CCTGATCGCC 
CACCTCCCGG ACCGCAGAGA GATCATCTCC TTTGGAAGCG GATATGGTGG GAACTCACTA 
CTCGGGAAGA AATGCTTTGC GTTGCGGATC GCCAGCCGTC TGGCTAAGGA GGAAGGGTGG 
CTGGCGGAGC ATATGCTGAT CCTGGGCATA ACTAACCCCG AAGGCAAGAA GAAATACCTG 
GCCGCAGCCT TCCCTAGTGC CTGTGGGAAG ACTAACTTGG CCATGATGAA CCCCAGCCTG 
CCCGGGTGGA AGGTCGAATG TGTGGGCGAT GACATTGCCT GGATGAAGTT TGATGCCCAA 
GGCAACTTAA GGGCTATCAA CCCAGAAAAC GGGTTTTTTG GAGTTGCTCC TGGCACCTCA 
GTGAAGACAA ATCCAAATGC CATTAAAACC ATCCAGAAAA ACACCATCTT CACCAACGTG 
GCCGAGACTA GCGATGGGGG TGTTTACTGG GAAGGCATCG ATGAGCCGCT GGCCCCGGGA 
GTCACCATCA CCTCCTGGAA GAACAAGGAG TGGAGACCGC AGGACGCGGA ACCATGTGCC 
CATCCCAACT CGAGATTCTG CACCCCTGCC AGCCAGTGCC CCATTATTGA CCCTGCCTGG 
GAATCTCCAG AAGGAGTACC CATTGAGGGT ATCATCTTTG GTGGCCGTAG ACCTGAAGGT 
GTCCCCCTTG TCTATGAAGC CCTCAGCTGG CAGCATGGGG TGTTTGTAGG AGCAGCCATG 
AGATCTGAGG CCACAGCTGC TGCAGAACAC AAGGGCAAGA TCATCATGCA CGACCCCTTT 
GCCATGCGAC CCTTCTTCGG CTACAACTTC GGCAAATACC TGGCCCACTG GCTGAGCATG 
GCCCACCGCC CAGCAGCCAA GTTGCCCAAG ATCTTCCATG TCAACTGGTT CCGGAAGGAC 
AAAGATGGCA AGTTCCTCTG GCCAGGCTTT GGCGAGAACT CCCGGGTGCT GGAGTGGATG 
TTCGGGCGGA TTGAAGGGGA AGACAGCGCC AAGCTCACGC CCATCGGCTA CATCCCTAAG 
GAAAACGCCT TGAACCTGAA AGGCCTGGGG GGCGTCAACG TGGAGGAGCT GTTTGGGATC 
TCTAAGGAGT TCTGGGAGAA GGAGGTGGAG GAGATCGACA GGTATCTGGA GGACCAGGTC 
AACACCGACC TCCCTTACGA AATTGAGAGG GAGCTCCGAG CCCTGAAACA GAGAATCAGC 
CAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035174.1
CDS	141..2009
Translation	MPPQLHNGLDFSAKVIQGSLDSLPQAVRKFVEGNAQLCQPEYIHICDGSEEEYGQLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGISVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDAEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPEGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKDGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYIPKENALNLKGLGGVNVEELFGISKEFWEKEVEEIDRYLEDQVNTDLPYEIERELRALKQRISQM

Target ORF information:

RefSeq Version	NM_011044.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011044.3

ORF Insert Sequence:

ATGCCTCCTC AGCTGCATAA CGGTCTGGAC TTCTCTGCCA AGGTCATCCA GGGCAGCCTC 
GACAGCCTGC CCCAGGCAGT GAGGAAGTTC GTGGAAGGCA ATGCTCAGCT GTGCCAGCCG 
GAGTATATCC ACATCTGCGA TGGCTCCGAG GAGGAGTACG GGCAGTTGCT GGCCCACATG 
CAGGAGGAGG GTGTCATCCG CAAGCTGAAG AAATATGACA ACTGTTGGCT GGCTCTCACT 
GACCCTCGAG ATGTGGCCAG GATCGAAAGC AAGACAGTCA TCATCACCCA AGAGCAGAGA 
GACACAGTGC CCATCCCCAA AACTGGCCTC AGCCAGCTGG GCCGCTGGAT GTCGGAAGAG 
GACTTTGAGA AAGCATTCAA CGCCAGGTTC CCAGGGTGCA TGAAAGGCCG CACCATGTAT 
GTCATCCCAT TCAGCATGGG GCCACTGGGC TCGCCGCTGG CCAAGATTGG TATTGAACTG 
ACAGACTCGC CCTATGTGGT GGCCAGCATG CGGATCATGA CTCGGATGGG CATATCTGTG 
CTGGAGGCCC TGGGAGATGG GGAGTTCATC AAGTGCCTGC ACTCTGTGGG GTGCCCTCTC 
CCCTTAAAAA AGCCTTTGGT CAACAACTGG GCCTGCAACC CTGAGCTGAC CCTGATCGCC 
CACCTCCCGG ACCGCAGAGA GATCATCTCC TTTGGAAGCG GATATGGTGG GAACTCACTA 
CTCGGGAAGA AATGCTTTGC GTTGCGGATC GCCAGCCGTC TGGCTAAGGA GGAAGGGTGG 
CTGGCGGAGC ATATGCTGAT CCTGGGCATA ACTAACCCCG AAGGCAAGAA GAAATACCTG 
GCCGCAGCCT TCCCTAGTGC CTGTGGGAAG ACTAACTTGG CCATGATGAA CCCCAGCCTG 
CCCGGGTGGA AGGTCGAATG TGTGGGCGAT GACATTGCCT GGATGAAGTT TGATGCCCAA 
GGCAACTTAA GGGCTATCAA CCCAGAAAAC GGGTTTTTTG GAGTTGCTCC TGGCACCTCA 
GTGAAGACAA ATCCAAATGC CATTAAAACC ATCCAGAAAA ACACCATCTT CACCAACGTG 
GCCGAGACTA GCGATGGGGG TGTTTACTGG GAAGGCATCG ATGAGCCGCT GGCCCCGGGA 
GTCACCATCA CCTCCTGGAA GAACAAGGAG TGGAGACCGC AGGACGCGGA ACCATGTGCC 
CATCCCAACT CGAGATTCTG CACCCCTGCC AGCCAGTGCC CCATTATTGA CCCTGCCTGG 
GAATCTCCAG AAGGAGTACC CATTGAGGGT ATCATCTTTG GTGGCCGTAG ACCTGAAGGT 
GTCCCCCTTG TCTATGAAGC CCTCAGCTGG CAGCATGGGG TGTTTGTAGG AGCAGCCATG 
AGATCTGAGG CCACAGCTGC TGCAGAACAC AAGGGCAAGA TCATCATGCA CGACCCCTTT 
GCCATGCGAC CCTTCTTCGG CTACAACTTC GGCAAATACC TGGCCCACTG GCTGAGCATG 
GCCCACCGCC CAGCAGCCAA GTTGCCCAAG ATCTTCCATG TCAACTGGTT CCGGAAGGAC 
AAAGATGGCA AGTTCCTCTG GCCAGGCTTT GGCGAGAACT CCCGGGTGCT GGAGTGGATG 
TTCGGGCGGA TTGAAGGGGA AGACAGCGCC AAGCTCACGC CCATCGGCTA CATCCCTAAG 
GAAAACGCCT TGAACCTGAA AGGCCTGGGG GGCGTCAACG TGGAGGAGCT GTTTGGGATC 
TCTAAGGAGT TCTGGGAGAA GGAGGTGGAG GAGATCGACA GGTATCTGGA GGACCAGGTC 
AACACCGACC TCCCTTACGA AATTGAGAGG GAGCTCCGAG CCCTGAAACA GAGAATCAGC 
CAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Macrophages with a deletion of the
The Journal of biological chemistry(2018 Jan)
Ko CW,Counihan D,Wu J,Hatzoglou M,Puchowicz MA,Croniger CM

A Pck1-directed glycogen metabolic program regulates formation and maintenance of memory CD8
Nature cell biology20(1)21-27(2018 Jan)
Ma R,Ji T,Zhang H,Dong W,Chen X,Xu P,Chen D,Liang X,Yin X,Liu Y,Ma J,Tang K,Zhang Y,Peng Y,Lu J,Zhang Y,Qin X,Cao X,Wan Y,Huang B

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Pck1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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