Otc cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Otc
Entrez Gene ID	18416
Full Name	ornithine transcarbamylase
Synonyms	AI265390,Sf,spf
General protein information	Preferred Names ornithine transcarbamylase Names ornithine carbamoyltransferase, mitochondrial OTCase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A1.1\|X 4.66 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247449.2	XP_011245751.1	ornithine carbamoyltransferase, mitochondrial isoform X1
XM_011247449.3	XP_011245751.1	ornithine carbamoyltransferase, mitochondrial isoform X1
NM_008769.4	NP_032795.1	ornithine carbamoyltransferase, mitochondrial precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	OTC	XP_537995.1
Gallus gallus (chicken)	OTC	NP_990241.1
Schizosaccharomyces pombe (fission yeast)	arg3	NP_593692.1
Neurospora crassa	NCU01667	XP_956873.2
Danio rerio (zebrafish)	otc	XP_001334671.1
Saccharomyces cerevisiae (baker's yeast)	ARG3	NP_012447.1
Xenopus tropicalis (tropical clawed frog)	otc	NP_001005795.1
Homo sapiens (human)	OTC	NP_000522.3
Kluyveromyces lactis	KLLA0F14366g	XP_455725.1
Magnaporthe oryzae (rice blast fungus)	MGG_01102	XP_003717832.1
Rattus norvegicus (Norway rat)	Otc	NP_037210.1
Bos taurus (cattle)	OTC	NP_803453.1
Mus musculus (house mouse)	Otc	NP_032795.1
Eremothecium gossypii	AGOS_AFR098W	NP_985645.1
Arabidopsis thaliana (thale cress)	OTC	NP_177667.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of liver-specific enhancer-promoter activity in the 3' untranslated region of the wild-type AAV2 genome.
Logan GJ, Dane AP, Hallwirth CV, Smyth CM, Wilkie EE, Amaya AK, Zhu E, Khandekar N, Ginn SL, Liao SHY, Cunningham SC, Sasaki N, Cabanes-Creus M, Tam PPL, Russell DW, Lisowski L, Alexander IE
Nature genetics49(8)1267-1273(2017 Aug)

AAV gene therapy corrects OTC deficiency and prevents liver fibrosis in aged OTC-knock out heterozygous mice.
Wang L, Bell P, Morizono H, He Z, Pumbo E, Yu H, White J, Batshaw ML, Wilson JM
Molecular genetics and metabolism120(4)299-305(2017 Apr)

A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice.
Yang Y, Wang L, Bell P, McMenamin D, He Z, White J, Yu H, Xu C, Morizono H, Musunuru K, Batshaw ML, Wilson JM
Nature biotechnology34(3)334-8(2016 Mar)

Functional characterization of the spf/ash splicing variation in OTC deficiency of mice and man.
Rivera-Barahona A, S?nchez-Alcudia R, Viecelli HM, R?fenacht V, P?rez B, Ugarte M, H?berle J, Th?ny B, Desviat LR
PloS one10(4)e0122966(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gene correction in adult OTC-deficient mice was lower and accompanied by larger deletions that ablated residual expression from the endogenous OTC gene, leading to diminished protein tolerance and lethal hyperammonemia on a chow diet
Title: A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice.

The corresponding OTC tissue enzyme activities were between 3-6% of normal control in mouse and human liver. The use of the cryptic splice sites was reproduced in minigenes carrying murine or human mutant sequences
Title: Functional characterization of the spf/ash splicing variation in OTC deficiency of mice and man.

Serum OCT seemed to reflect tumor necrosis factor-alpha-mediated hepatic damage in diabetic obese mice and could be useful in the application for non-alcoholic fatty liver disease with features of metabolic syndrome, such as obesity and diabetes.
Title: Serum ornithine carbamyltransferase reflects hepatic damage in diabetic obese mice.

Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
Title: Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.

Phenotypic correction of OTC deficiency using low-dose helper-dependent adenoviral vectors is reported.
Title: Phenotypic correction of ornithine transcarbamylase deficiency using low dose helper-dependent adenoviral vectors.

The following Otc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Otc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16524	NM_008769.4 Latest version!	Mus musculus ornithine transcarbamylase (Otc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu68369	XM_011247449.3 Latest version!	Mus musculus ornithine transcarbamylase (Otc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu68369	XM_011247449.2	Mus musculus ornithine transcarbamylase (Otc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Otc ( XM_011247449.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245751.1 Mus musculus ornithine transcarbamylase (Otc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68369
Clone ID Related Accession (Same CDS sequence)	XM_011247449.2 , XM_011247449.3
Accession Version	XM_011247449.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ornithine carbamoyltransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTA ATTTGAGGAT CCTGCTCAAC AATGCAGCTC TTAGAAAGGG TCACACTTCT 
GTGGTTCGAC ATTTTTGGTG TGGGAAGCCA GTCCAAAGTC AAGTACAGCT GAAAGGCCGT 
GACCTCCTCA CCTTGAAGAA CTTCACAGGA GAGGAGATTC AGTACATGCT ATGGCTCTCT 
GCAGATCTGA AATTCAGGAT CAAGCAGAAA GGAGAATATT TACCTTTATT GCAAGGGAAA 
TCCTTAGGAA TGATTTTTGA GAAAAGAAGT ACTCGAACAA GACTGTCCAC AGAAACAGGC 
TTTGCTCTGC TGGGAGGACA CCCTTCCTTT CTTACCACAC AAGACATTCA CTTGGGTGTG 
AATGAAAGTC TCACAGACAC CGCTCGTGTC TTATCTAGCA TGACAGATGC AGTGTTAGCT 
CGAGTGTATA AACAATCAGA TCTGGACACC CTGGCTAAAG AAGCATCCAT CCCAATTGTC 
AATGGACTGT CAGACTTGTA TCATCCTATC CAGATCCTGG CTGATTACCT TACACTCCAG 
GAACACTATG GCTCTCTCAA AGGTCTTACC CTCAGCTGGA TAGGGGATGG GAACAATATC 
TTGCACTCTA TCATGATGAG TGCTGCAAAA TTCGGGATGC ACCTTCAAGC AGCTACTCCA 
AAGGGTTATG AGCCAGATCC TAATATAGTC AAGCTAGCAG AGCAGTATGC CAAGGAGAAT 
GGTACCAAGT TGTCAATGAC AAATGATCCA CTGGAAGCAG CACGTGGAGG CAATGTATTA 
ATTACAGATA CTTGGATAAG CATGGGACAA GAGGATGAGA AGAAAAAGCG TCTTCAAGCT 
TTCCAAGGTT ACCAGGTTAC GATGAAGACT GCCAAAGTGG CTGCGTCTGA CTGGACATTT 
TTACACTGTT TGCCTAGAAA GCCAGAAGAA GTGGATGATG AAGTATTTTA TTCTCCACGG 
TCATTAGTGT TCCCAGAGGC AGAGAATAGA AAGTGGACAA TCATGGTCTC ACTGAGATCT 
CACCATGCCC CTTGCCTTGG ATCACTCTTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245751.1
CDS	142..1197
Translation	MLSNLRILLNNAALRKGHTSVVRHFWCGKPVQSQVQLKGRDLLTLKNFTGEEIQYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPSFLTTQDIHLGVNESLTDTARVLSSMTDAVLARVYKQSDLDTLAKEASIPIVNGLSDLYHPIQILADYLTLQEHYGSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDPNIVKLAEQYAKENGTKLSMTNDPLEAARGGNVLITDTWISMGQEDEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMVSLRSHHAPCLGSLFL

Target ORF information:

RefSeq Version	XM_011247449.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ornithine transcarbamylase (Otc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247449.2

ORF Insert Sequence:

ATGCTGTCTA ATTTGAGGAT CCTGCTCAAC AATGCAGCTC TTAGAAAGGG TCACACTTCT 
GTGGTTCGAC ATTTTTGGTG TGGGAAGCCA GTCCAAAGTC AAGTACAGCT GAAAGGCCGT 
GACCTCCTCA CCTTGAAGAA CTTCACAGGA GAGGAGATTC AGTACATGCT ATGGCTCTCT 
GCAGATCTGA AATTCAGGAT CAAGCAGAAA GGAGAATATT TACCTTTATT GCAAGGGAAA 
TCCTTAGGAA TGATTTTTGA GAAAAGAAGT ACTCGAACAA GACTGTCCAC AGAAACAGGC 
TTTGCTCTGC TGGGAGGACA CCCTTCCTTT CTTACCACAC AAGACATTCA CTTGGGTGTG 
AATGAAAGTC TCACAGACAC CGCTCGTGTC TTATCTAGCA TGACAGATGC AGTGTTAGCT 
CGAGTGTATA AACAATCAGA TCTGGACACC CTGGCTAAAG AAGCATCCAT CCCAATTGTC 
AATGGACTGT CAGACTTGTA TCATCCTATC CAGATCCTGG CTGATTACCT TACACTCCAG 
GAACACTATG GCTCTCTCAA AGGTCTTACC CTCAGCTGGA TAGGGGATGG GAACAATATC 
TTGCACTCTA TCATGATGAG TGCTGCAAAA TTCGGGATGC ACCTTCAAGC AGCTACTCCA 
AAGGGTTATG AGCCAGATCC TAATATAGTC AAGCTAGCAG AGCAGTATGC CAAGGAGAAT 
GGTACCAAGT TGTCAATGAC AAATGATCCA CTGGAAGCAG CACGTGGAGG CAATGTATTA 
ATTACAGATA CTTGGATAAG CATGGGACAA GAGGATGAGA AGAAAAAGCG TCTTCAAGCT 
TTCCAAGGTT ACCAGGTTAC GATGAAGACT GCCAAAGTGG CTGCGTCTGA CTGGACATTT 
TTACACTGTT TGCCTAGAAA GCCAGAAGAA GTGGATGATG AAGTATTTTA TTCTCCACGG 
TCATTAGTGT TCCCAGAGGC AGAGAATAGA AAGTGGACAA TCATGGTCTC ACTGAGATCT 
CACCATGCCC CTTGCCTTGG ATCACTCTTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otc ( NM_008769.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032795.1 Mus musculus ornithine transcarbamylase (Otc), mRNA.

Price & Availability↑

CloneID	OMu16524
Clone ID Related Accession (Same CDS sequence)	NM_008769.4
Accession Version	NM_008769.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	ornithine carbamoyltransferase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671042.10, BY090290.1 and AK050930.1. On Jul 18, 2015 this sequence version replaced NM_008769.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK050930.1, M17030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCTGTCTA ATTTGAGGAT CCTGCTCAAC AATGCAGCTC TTAGAAAGGG TCACACTTCT 
GTGGTTCGAC ATTTTTGGTG TGGGAAGCCA GTCCAAAGTC AAGTACAGCT GAAAGGCCGT 
GACCTCCTCA CCTTGAAGAA CTTCACAGGA GAGGAGATTC AGTACATGCT ATGGCTCTCT 
GCAGATCTGA AATTCAGGAT CAAGCAGAAA GGAGAATATT TACCTTTATT GCAAGGGAAA 
TCCTTAGGAA TGATTTTTGA GAAAAGAAGT ACTCGAACAA GACTGTCCAC AGAAACAGGC 
TTTGCTCTGC TGGGAGGACA CCCTTCCTTT CTTACCACAC AAGACATTCA CTTGGGTGTG 
AATGAAAGTC TCACAGACAC CGCTCGTGTC TTATCTAGCA TGACAGATGC AGTGTTAGCT 
CGAGTGTATA AACAATCAGA TCTGGACACC CTGGCTAAAG AAGCATCCAT CCCAATTGTC 
AATGGACTGT CAGACTTGTA TCATCCTATC CAGATCCTGG CTGATTACCT TACACTCCAG 
GAACACTATG GCTCTCTCAA AGGTCTTACC CTCAGCTGGA TAGGGGATGG GAACAATATC 
TTGCACTCTA TCATGATGAG TGCTGCAAAA TTCGGGATGC ACCTTCAAGC AGCTACTCCA 
AAGGGTTATG AGCCAGATCC TAATATAGTC AAGCTAGCAG AGCAGTATGC CAAGGAGAAT 
GGTACCAAGT TGTCAATGAC AAATGATCCA CTGGAAGCAG CACGTGGAGG CAATGTATTA 
ATTACAGATA CTTGGATAAG CATGGGACAA GAGGATGAGA AGAAAAAGCG TCTTCAAGCT 
TTCCAAGGTT ACCAGGTTAC GATGAAGACT GCCAAAGTGG CTGCGTCTGA CTGGACATTT 
TTACACTGTT TGCCTAGAAA GCCAGAAGAA GTGGATGATG AAGTATTTTA TTCTCCACGG 
TCATTAGTGT TCCCAGAGGC AGAGAATAGA AAGTGGACAA TCATGGCTGT CATGGTATCC 
CTGCTGACAG ACTACTCACC TGTGCTCCAG AAGCCAAAGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032795.1
CDS	133..1197
Translation	MLSNLRILLNNAALRKGHTSVVRHFWCGKPVQSQVQLKGRDLLTLKNFTGEEIQYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPSFLTTQDIHLGVNESLTDTARVLSSMTDAVLARVYKQSDLDTLAKEASIPIVNGLSDLYHPIQILADYLTLQEHYGSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDPNIVKLAEQYAKENGTKLSMTNDPLEAARGGNVLITDTWISMGQEDEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPVLQKPKF

Target ORF information:

RefSeq Version	NM_008769.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ornithine transcarbamylase (Otc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008769.4

ORF Insert Sequence:

ATGCTGTCTA ATTTGAGGAT CCTGCTCAAC AATGCAGCTC TTAGAAAGGG TCACACTTCT 
GTGGTTCGAC ATTTTTGGTG TGGGAAGCCA GTCCAAAGTC AAGTACAGCT GAAAGGCCGT 
GACCTCCTCA CCTTGAAGAA CTTCACAGGA GAGGAGATTC AGTACATGCT ATGGCTCTCT 
GCAGATCTGA AATTCAGGAT CAAGCAGAAA GGAGAATATT TACCTTTATT GCAAGGGAAA 
TCCTTAGGAA TGATTTTTGA GAAAAGAAGT ACTCGAACAA GACTGTCCAC AGAAACAGGC 
TTTGCTCTGC TGGGAGGACA CCCTTCCTTT CTTACCACAC AAGACATTCA CTTGGGTGTG 
AATGAAAGTC TCACAGACAC CGCTCGTGTC TTATCTAGCA TGACAGATGC AGTGTTAGCT 
CGAGTGTATA AACAATCAGA TCTGGACACC CTGGCTAAAG AAGCATCCAT CCCAATTGTC 
AATGGACTGT CAGACTTGTA TCATCCTATC CAGATCCTGG CTGATTACCT TACACTCCAG 
GAACACTATG GCTCTCTCAA AGGTCTTACC CTCAGCTGGA TAGGGGATGG GAACAATATC 
TTGCACTCTA TCATGATGAG TGCTGCAAAA TTCGGGATGC ACCTTCAAGC AGCTACTCCA 
AAGGGTTATG AGCCAGATCC TAATATAGTC AAGCTAGCAG AGCAGTATGC CAAGGAGAAT 
GGTACCAAGT TGTCAATGAC AAATGATCCA CTGGAAGCAG CACGTGGAGG CAATGTATTA 
ATTACAGATA CTTGGATAAG CATGGGACAA GAGGATGAGA AGAAAAAGCG TCTTCAAGCT 
TTCCAAGGTT ACCAGGTTAC GATGAAGACT GCCAAAGTGG CTGCGTCTGA CTGGACATTT 
TTACACTGTT TGCCTAGAAA GCCAGAAGAA GTGGATGATG AAGTATTTTA TTCTCCACGG 
TCATTAGTGT TCCCAGAGGC AGAGAATAGA AAGTGGACAA TCATGGCTGT CATGGTATCC 
CTGCTGACAG ACTACTCACC TGTGCTCCAG AAGCCAAAGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otc ( XM_011247449.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245751.1 Mus musculus ornithine transcarbamylase (Otc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68369
Clone ID Related Accession (Same CDS sequence)	XM_011247449.2 , XM_011247449.3
Accession Version	XM_011247449.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ornithine carbamoyltransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTA ATTTGAGGAT CCTGCTCAAC AATGCAGCTC TTAGAAAGGG TCACACTTCT 
GTGGTTCGAC ATTTTTGGTG TGGGAAGCCA GTCCAAAGTC AAGTACAGCT GAAAGGCCGT 
GACCTCCTCA CCTTGAAGAA CTTCACAGGA GAGGAGATTC AGTACATGCT ATGGCTCTCT 
GCAGATCTGA AATTCAGGAT CAAGCAGAAA GGAGAATATT TACCTTTATT GCAAGGGAAA 
TCCTTAGGAA TGATTTTTGA GAAAAGAAGT ACTCGAACAA GACTGTCCAC AGAAACAGGC 
TTTGCTCTGC TGGGAGGACA CCCTTCCTTT CTTACCACAC AAGACATTCA CTTGGGTGTG 
AATGAAAGTC TCACAGACAC CGCTCGTGTC TTATCTAGCA TGACAGATGC AGTGTTAGCT 
CGAGTGTATA AACAATCAGA TCTGGACACC CTGGCTAAAG AAGCATCCAT CCCAATTGTC 
AATGGACTGT CAGACTTGTA TCATCCTATC CAGATCCTGG CTGATTACCT TACACTCCAG 
GAACACTATG GCTCTCTCAA AGGTCTTACC CTCAGCTGGA TAGGGGATGG GAACAATATC 
TTGCACTCTA TCATGATGAG TGCTGCAAAA TTCGGGATGC ACCTTCAAGC AGCTACTCCA 
AAGGGTTATG AGCCAGATCC TAATATAGTC AAGCTAGCAG AGCAGTATGC CAAGGAGAAT 
GGTACCAAGT TGTCAATGAC AAATGATCCA CTGGAAGCAG CACGTGGAGG CAATGTATTA 
ATTACAGATA CTTGGATAAG CATGGGACAA GAGGATGAGA AGAAAAAGCG TCTTCAAGCT 
TTCCAAGGTT ACCAGGTTAC GATGAAGACT GCCAAAGTGG CTGCGTCTGA CTGGACATTT 
TTACACTGTT TGCCTAGAAA GCCAGAAGAA GTGGATGATG AAGTATTTTA TTCTCCACGG 
TCATTAGTGT TCCCAGAGGC AGAGAATAGA AAGTGGACAA TCATGGTCTC ACTGAGATCT 
CACCATGCCC CTTGCCTTGG ATCACTCTTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245751.1
CDS	208..1263
Translation	MLSNLRILLNNAALRKGHTSVVRHFWCGKPVQSQVQLKGRDLLTLKNFTGEEIQYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPSFLTTQDIHLGVNESLTDTARVLSSMTDAVLARVYKQSDLDTLAKEASIPIVNGLSDLYHPIQILADYLTLQEHYGSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDPNIVKLAEQYAKENGTKLSMTNDPLEAARGGNVLITDTWISMGQEDEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMVSLRSHHAPCLGSLFL

Target ORF information:

RefSeq Version	XM_011247449.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ornithine transcarbamylase (Otc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247449.3

ORF Insert Sequence:

ATGCTGTCTA ATTTGAGGAT CCTGCTCAAC AATGCAGCTC TTAGAAAGGG TCACACTTCT 
GTGGTTCGAC ATTTTTGGTG TGGGAAGCCA GTCCAAAGTC AAGTACAGCT GAAAGGCCGT 
GACCTCCTCA CCTTGAAGAA CTTCACAGGA GAGGAGATTC AGTACATGCT ATGGCTCTCT 
GCAGATCTGA AATTCAGGAT CAAGCAGAAA GGAGAATATT TACCTTTATT GCAAGGGAAA 
TCCTTAGGAA TGATTTTTGA GAAAAGAAGT ACTCGAACAA GACTGTCCAC AGAAACAGGC 
TTTGCTCTGC TGGGAGGACA CCCTTCCTTT CTTACCACAC AAGACATTCA CTTGGGTGTG 
AATGAAAGTC TCACAGACAC CGCTCGTGTC TTATCTAGCA TGACAGATGC AGTGTTAGCT 
CGAGTGTATA AACAATCAGA TCTGGACACC CTGGCTAAAG AAGCATCCAT CCCAATTGTC 
AATGGACTGT CAGACTTGTA TCATCCTATC CAGATCCTGG CTGATTACCT TACACTCCAG 
GAACACTATG GCTCTCTCAA AGGTCTTACC CTCAGCTGGA TAGGGGATGG GAACAATATC 
TTGCACTCTA TCATGATGAG TGCTGCAAAA TTCGGGATGC ACCTTCAAGC AGCTACTCCA 
AAGGGTTATG AGCCAGATCC TAATATAGTC AAGCTAGCAG AGCAGTATGC CAAGGAGAAT 
GGTACCAAGT TGTCAATGAC AAATGATCCA CTGGAAGCAG CACGTGGAGG CAATGTATTA 
ATTACAGATA CTTGGATAAG CATGGGACAA GAGGATGAGA AGAAAAAGCG TCTTCAAGCT 
TTCCAAGGTT ACCAGGTTAC GATGAAGACT GCCAAAGTGG CTGCGTCTGA CTGGACATTT 
TTACACTGTT TGCCTAGAAA GCCAGAAGAA GTGGATGATG AAGTATTTTA TTCTCCACGG 
TCATTAGTGT TCCCAGAGGC AGAGAATAGA AAGTGGACAA TCATGGTCTC ACTGAGATCT 
CACCATGCCC CTTGCCTTGG ATCACTCTTC TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of liver-specific enhancer-promoter activity in the 3' untranslated region of the wild-type AAV2 genome.
Nature genetics49(8)1267-1273(2017 Aug)
Logan GJ,Dane AP,Hallwirth CV,Smyth CM,Wilkie EE,Amaya AK,Zhu E,Khandekar N,Ginn SL,Liao SHY,Cunningham SC,Sasaki N,Cabanes-Creus M,Tam PPL,Russell DW,Lisowski L,Alexander IE

AAV gene therapy corrects OTC deficiency and prevents liver fibrosis in aged OTC-knock out heterozygous mice.
Molecular genetics and metabolism120(4)299-305(2017 Apr)
Wang L,Bell P,Morizono H,He Z,Pumbo E,Yu H,White J,Batshaw ML,Wilson JM

A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice.
Nature biotechnology34(3)334-8(2016 Mar)
Yang Y,Wang L,Bell P,McMenamin D,He Z,White J,Yu H,Xu C,Morizono H,Musunuru K,Batshaw ML,Wilson JM

Functional characterization of the spf/ash splicing variation in OTC deficiency of mice and man.
PloS one10(4)e0122966(2015)
Rivera-Barahona A,S?nchez-Alcudia R,Viecelli HM,R?fenacht V,P?rez B,Ugarte M,H?berle J,Th?ny B,Desviat LR

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Otc cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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