Npc1 cDNA ORF clone, Mus musculus(house mouse)

The following Npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu79770 XM_017317849.2
Latest version!
Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu21708 NM_008720.2
Latest version!
Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $748.30
$1069.00
View Old Accession Versions >>
OMu79770 XM_017317849.1 Mus musculus Niemann-Pick type C1 (Npc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu79770
    Clone ID Related Accession (Same CDS sequence) XM_017317849.1 , XM_017317849.2
    Accession Version XM_017317849.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Niemann-Pick C1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
    TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
    AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
    GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
    CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
    TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
    CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
    GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
    CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
    GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
    GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
    AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
    CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
    AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
    TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG
    TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC
    TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG
    TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC
    ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG
    TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT
    TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA
    CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC
    CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC
    GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT
    ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC
    GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC
    TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG
    TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT
    GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG
    GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT
    TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG
    TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC
    ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC
    CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG
    CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC
    ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT
    CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT
    GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT
    CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC
    CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC
    AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC
    AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG
    TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT
    CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG
    TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA
    CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG
    CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC
    TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC
    GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT
    CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG
    TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA
    GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC
    ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC
    AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT
    GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC
    AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG
    TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG
    TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC
    ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC
    CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC
    ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG
    ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA
    TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC
    ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173338.1
    CDS196..4026
    Translation

    Target ORF information:

    RefSeq Version XM_017317849.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Niemann-Pick type C1 (Npc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
    TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
    AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
    GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
    CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
    TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
    CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
    GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
    CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
    GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
    GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
    AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
    CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
    AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
    TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG
    TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC
    TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG
    TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC
    ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG
    TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT
    TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA
    CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC
    CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC
    GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT
    ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC
    GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC
    TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG
    TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT
    GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG
    GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT
    TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG
    TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC
    ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC
    CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG
    CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC
    ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT
    CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT
    GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT
    CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC
    CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC
    AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC
    AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG
    TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT
    CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG
    TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA
    CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG
    CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC
    TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC
    GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT
    CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG
    TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA
    GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC
    ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC
    AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT
    GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC
    AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG
    TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG
    TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC
    ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC
    CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC
    ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG
    ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA
    TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC
    ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79770
    Clone ID Related Accession (Same CDS sequence) XM_017317849.1 , XM_017317849.2
    Accession Version XM_017317849.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NPC intracellular cholesterol transporter 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
    TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
    AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
    GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
    CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
    TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
    CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
    GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
    CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
    GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
    GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
    AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
    CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
    AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
    TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG
    TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC
    TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG
    TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC
    ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG
    TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT
    TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA
    CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC
    CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC
    GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT
    ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC
    GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC
    TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG
    TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT
    GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG
    GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT
    TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG
    TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC
    ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC
    CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG
    CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC
    ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT
    CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT
    GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT
    CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC
    CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC
    AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC
    AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG
    TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT
    CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG
    TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA
    CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG
    CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC
    TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC
    GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT
    CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG
    TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA
    GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC
    ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC
    AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT
    GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC
    AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG
    TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG
    TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC
    ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC
    CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC
    ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG
    ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA
    TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC
    ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173338.1
    CDS270..4100
    Translation

    Target ORF information:

    RefSeq Version XM_017317849.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317849.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
    TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
    AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
    GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
    CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
    TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
    CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
    GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
    CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
    GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
    GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
    AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
    CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
    AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
    TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GGCGGTACTT TGTGTCTGAG
    TACACTCCCA TTGACAGTAA CATCGCCTTT TCTGTGAATA GCAGTGACAA AGGGGAAGCC
    TCATGCTGTG ACCCACTTGG TGCAGCATTT GATGACTGTC TGAGGCGCAT GTTCACAAAG
    TGGGGGGCTT TCTGTGTCCG AAATCCCACC TGCATCATTT TCTTCTCATT GGCCTTCATC
    ACTGTGTGCT CTTCTGGCCT GGTATTTGTC CAGGTCACCA CCAATCCTGT AGAGCTCTGG
    TCAGCCCCTC ACAGTCAGGC CCGCTTGGAA AAGGAGTACT TTGACAAGCA CTTTGGGCCT
    TTCTTTCGCA CGGAGCAGCT TATCATCCAG GCCCCCAACA CCAGTGTTCA TATCTACGAA
    CCGTACCCCG CAGGAGCCGA TGTGCCCTTC GGGCCTCCAT TGAACAAAGA GATTCTGCAC
    CAGGTTCTGG ACTTACAGAT CGCCATTGAA AGCATCACCG CATCTTACAA CAATGAAACC
    GTGACACTGC AGGACATCTG TGTGGCCCCC CTCTCTCCAT ACAACAAGAA CTGCACCATT
    ATGAGTGTGT TAAATTACTT CCAGAACAGC CATGCGGTGC TGGACAGCCA AGTAGGCGAC
    GACTTCTATA TCTACGCTGA TTACCACACA CACTTTCTGT ACTGTGTACG GGCTCCCGCC
    TCCTTGAATG ATACGAGTTT GCTCCACGGT CCTTGCCTGG GTACATTTGG AGGACCGGTG
    TTCCCGTGGC TTGTGTTGGG TGGCTATGAT GATCAGAACT ACAACAATGC CACCGCGCTT
    GTGATCACCT TCCCCGTGAA TAATTACTAC AATGACACAG AGAGGCTCCA GAGGGCCTGG
    GCCTGGGAGA AAGAGTTTAT TAGTTTTGTG AAAAACTACA AGAATCCAAA TCTGACCATT
    TCTTTCACTG CTGAGCGAAG CATCGAAGAT GAGCTCAATC GGGAAAGTAA CAGTGACGTG
    TTCACCGTCA TCATCAGCTA CGTCGTGATG TTTCTGTACA TTTCCCTCGC CCTGGGTCAC
    ATCCAGAGCT GCAGCAGGCT CCTGGTGGAT TCTAAGATCT CGCTGGGCAT TGCGGGGATC
    CTGATCGTGC TAAGCTCGGT GGCCTGCTCT CTGGGCATCT TCAGCTACAT GGGGATGCCG
    CTGACCCTCA TCGTCATTGA GGTCATCCCA TTCCTGGTGC TGGCTGTCGG GGTGGACAAC
    ATCTTCATTC TAGTGCAGAC CTACCAGAGA GATGAGCGTC TTCAGGAGGA AACGCTGGAT
    CAGCAGCTGG GCAGGATCCT TGGAGAAGTG GCCCCGACCA TGTTCCTTTC ATCCTTTTCT
    GAGACCTCAG CATTTTTCTT TGGGGCGCTG TCCTCGATGC CAGCTGTGCA CACCTTCTCT
    CTGTTTGCGG GAATGGCCGT CCTCATTGAC TTCCTCCTTC AGATTACCTG CTTTGTGAGC
    CTGTTGGGGT TAGATATTAA GAGGCAAGAG AAAAACCATC TGGACATCCT GTGCTGTGTC
    AGAGGCGCTG ACGACGGACA AGGTAGCCAC GCCTCCGAAA GCTACCTGTT TCGCTTCTTC
    AAAAACTACT TTGCCCCCCT TCTGCTGAAG GACTGGCTGA GGCCAATTGT GGTAGCGGTG
    TTTGTGGGCG TTCTGTCATT CAGTGTTGCG GTGGTGAACA AAGTAGACAT CGGGTTGGAT
    CAGTCTCTCT CAATGCCAAA CGATTCGTAT GTGATTGACT ATTTCAAATC ACTCGCTCAG
    TACCTGCACT CGGGCCCACC CGTGTACTTT GTCCTGGAGG AAGGCTATAA CTACAGTTCA
    CGCAAAGGGC AGAACATGGT GTGCGGCGGC ATGGGCTGTG ACAATGACTC CCTGGTGCAG
    CAGATATTTA ACGCAGCTGA GCTGGACACC TACACCCGAG TAGGCTTCGC CCCCTCGTCC
    TGGATCGATG ACTACTTTGA CTGGGTCTCG CCACAGTCGT CCTGCTGCAG ACTCTACAAC
    GTCACTCACC AGTTCTGCAA TGCTTCTGTG ATGGACCCAA CCTGTGTCCG CTGCAGACCT
    CTGACTCCAG AGGGTAAACA GAGGCCTCAG GGGAAAGAAT TCATGAAATT CCTGCCCATG
    TTCCTTTCTG ATAACCCCAA CCCCAAGTGC GGCAAAGGGG GACATGCTGC TTACGGTTCA
    GCTGTTAACA TTGTGGGAGA TGACACTTAC ATTGGGGCCA CTTACTTCAT GACCTACCAC
    ACCATACTTA AGACCTCCGC TGACTATACT GATGCCATGA AGAAAGCTCG GCTAATAGCC
    AGTAACATCA CGGAAACCAT GCGTTCTAAG GGGAGTGACT ACCGCGTATT CCCTTACAGT
    GTGTTCTACG TCTTCTATGA ACAGTACCTG ACCATTATTG ATGACACCAT CTTTAACCTC
    AGTGTGTCTC TGGGCTCCAT ATTTCTGGTG ACCTTGGTGG TTCTGGGCTG TGAGCTGTGG
    TCTGCGGTCA TCATGTGTAT CACCATAGCC ATGATCCTGG TCAACATGTT CGGTGTCATG
    TGGCTGTGGG GCATCAGTCT GAATGCGGTC TCCTTGGTCA ACTTGGTGAT GAGCTGCGGC
    ATTTCTGTGG AGTTCTGCAG CCATATAACG AGAGCATTCA CCATGAGTAC CAAAGGAAGC
    CGAGTGAGCC GGGCGGAAGA GGCACTGGCC CACATGGGTA GTTCTGTATT CAGTGGAATC
    ACACTTACGA AATTTGGAGG GATCGTGGTG TTAGCCTTTG CCAAATCTCA AATTTTTGAG
    ATATTTTACT TCAGGATGTA CTTAGCCATG GTCTTACTCG GAGCCACTCA TGGACTAATA
    TTTCTTCCCG TCTTACTCAG TTACATAGGG CCGTCGGTGA ATAAAGCTAA AAGACACACC
    ACTTACGAGC GCTACAGAGG GACAGAGAGA GAACGGCTCC TCAATTTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21708
    Clone ID Related Accession (Same CDS sequence) NM_008720.2
    Accession Version NM_008720.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product NPC intracellular cholesterol transporter 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052437.1, CF585180.1 and AK003935.1. On Mar 7, 2006 this sequence version replaced NM_008720.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052437.1, AF003348.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
    TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
    AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
    GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
    CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
    TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
    CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
    GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
    CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
    GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
    GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
    AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
    CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
    AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
    TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GAAGGCGGTA CTTTGTGTCT
    GAGTACACTC CCATTGACAG TAACATCGCC TTTTCTGTGA ATAGCAGTGA CAAAGGGGAA
    GCCTCATGCT GTGACCCACT TGGTGCAGCA TTTGATGACT GTCTGAGGCG CATGTTCACA
    AAGTGGGGGG CTTTCTGTGT CCGAAATCCC ACCTGCATCA TTTTCTTCTC ATTGGCCTTC
    ATCACTGTGT GCTCTTCTGG CCTGGTATTT GTCCAGGTCA CCACCAATCC TGTAGAGCTC
    TGGTCAGCCC CTCACAGTCA GGCCCGCTTG GAAAAGGAGT ACTTTGACAA GCACTTTGGG
    CCTTTCTTTC GCACGGAGCA GCTTATCATC CAGGCCCCCA ACACCAGTGT TCATATCTAC
    GAACCGTACC CCGCAGGAGC CGATGTGCCC TTCGGGCCTC CATTGAACAA AGAGATTCTG
    CACCAGGTTC TGGACTTACA GATCGCCATT GAAAGCATCA CCGCATCTTA CAACAATGAA
    ACCGTGACAC TGCAGGACAT CTGTGTGGCC CCCCTCTCTC CATACAACAA GAACTGCACC
    ATTATGAGTG TGTTAAATTA CTTCCAGAAC AGCCATGCGG TGCTGGACAG CCAAGTAGGC
    GACGACTTCT ATATCTACGC TGATTACCAC ACACACTTTC TGTACTGTGT ACGGGCTCCC
    GCCTCCTTGA ATGATACGAG TTTGCTCCAC GGTCCTTGCC TGGGTACATT TGGAGGACCG
    GTGTTCCCGT GGCTTGTGTT GGGTGGCTAT GATGATCAGA ACTACAACAA TGCCACCGCG
    CTTGTGATCA CCTTCCCCGT GAATAATTAC TACAATGACA CAGAGAGGCT CCAGAGGGCC
    TGGGCCTGGG AGAAAGAGTT TATTAGTTTT GTGAAAAACT ACAAGAATCC AAATCTGACC
    ATTTCTTTCA CTGCTGAGCG AAGCATCGAA GATGAGCTCA ATCGGGAAAG TAACAGTGAC
    GTGTTCACCG TCATCATCAG CTACGTCGTG ATGTTTCTGT ACATTTCCCT CGCCCTGGGT
    CACATCCAGA GCTGCAGCAG GCTCCTGGTG GATTCTAAGA TCTCGCTGGG CATTGCGGGG
    ATCCTGATCG TGCTAAGCTC GGTGGCCTGC TCTCTGGGCA TCTTCAGCTA CATGGGGATG
    CCGCTGACCC TCATCGTCAT TGAGGTCATC CCATTCCTGG TGCTGGCTGT CGGGGTGGAC
    AACATCTTCA TTCTAGTGCA GACCTACCAG AGAGATGAGC GTCTTCAGGA GGAAACGCTG
    GATCAGCAGC TGGGCAGGAT CCTTGGAGAA GTGGCCCCGA CCATGTTCCT TTCATCCTTT
    TCTGAGACCT CAGCATTTTT CTTTGGGGCG CTGTCCTCGA TGCCAGCTGT GCACACCTTC
    TCTCTGTTTG CGGGAATGGC CGTCCTCATT GACTTCCTCC TTCAGATTAC CTGCTTTGTG
    AGCCTGTTGG GGTTAGATAT TAAGAGGCAA GAGAAAAACC ATCTGGACAT CCTGTGCTGT
    GTCAGAGGCG CTGACGACGG ACAAGGTAGC CACGCCTCCG AAAGCTACCT GTTTCGCTTC
    TTCAAAAACT ACTTTGCCCC CCTTCTGCTG AAGGACTGGC TGAGGCCAAT TGTGGTAGCG
    GTGTTTGTGG GCGTTCTGTC ATTCAGTGTT GCGGTGGTGA ACAAAGTAGA CATCGGGTTG
    GATCAGTCTC TCTCAATGCC AAACGATTCG TATGTGATTG ACTATTTCAA ATCACTCGCT
    CAGTACCTGC ACTCGGGCCC ACCCGTGTAC TTTGTCCTGG AGGAAGGCTA TAACTACAGT
    TCACGCAAAG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GTGACAATGA CTCCCTGGTG
    CAGCAGATAT TTAACGCAGC TGAGCTGGAC ACCTACACCC GAGTAGGCTT CGCCCCCTCG
    TCCTGGATCG ATGACTACTT TGACTGGGTC TCGCCACAGT CGTCCTGCTG CAGACTCTAC
    AACGTCACTC ACCAGTTCTG CAATGCTTCT GTGATGGACC CAACCTGTGT CCGCTGCAGA
    CCTCTGACTC CAGAGGGTAA ACAGAGGCCT CAGGGGAAAG AATTCATGAA ATTCCTGCCC
    ATGTTCCTTT CTGATAACCC CAACCCCAAG TGCGGCAAAG GGGGACATGC TGCTTACGGT
    TCAGCTGTTA ACATTGTGGG AGATGACACT TACATTGGGG CCACTTACTT CATGACCTAC
    CACACCATAC TTAAGACCTC CGCTGACTAT ACTGATGCCA TGAAGAAAGC TCGGCTAATA
    GCCAGTAACA TCACGGAAAC CATGCGTTCT AAGGGGAGTG ACTACCGCGT ATTCCCTTAC
    AGTGTGTTCT ACGTCTTCTA TGAACAGTAC CTGACCATTA TTGATGACAC CATCTTTAAC
    CTCAGTGTGT CTCTGGGCTC CATATTTCTG GTGACCTTGG TGGTTCTGGG CTGTGAGCTG
    TGGTCTGCGG TCATCATGTG TATCACCATA GCCATGATCC TGGTCAACAT GTTCGGTGTC
    ATGTGGCTGT GGGGCATCAG TCTGAATGCG GTCTCCTTGG TCAACTTGGT GATGAGCTGC
    GGCATTTCTG TGGAGTTCTG CAGCCATATA ACGAGAGCAT TCACCATGAG TACCAAAGGA
    AGCCGAGTGA GCCGGGCGGA AGAGGCACTG GCCCACATGG GTAGTTCTGT ATTCAGTGGA
    ATCACACTTA CGAAATTTGG AGGGATCGTG GTGTTAGCCT TTGCCAAATC TCAAATTTTT
    GAGATATTTT ACTTCAGGAT GTACTTAGCC ATGGTCTTAC TCGGAGCCAC TCATGGACTA
    ATATTTCTTC CCGTCTTACT CAGTTACATA GGGCCGTCGG TGAATAAAGC TAAAAGACAC
    ACCACTTACG AGCGCTACAG AGGGACAGAG AGAGAACGGC TCCTCAATTT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032746.2
    CDS150..3983
    Translation

    Target ORF information:

    RefSeq Version NM_008720.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus NPC intracellular cholesterol transporter 1 (Npc1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008720.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGTGCGC ACCACCCGGC CCTCGGCCTG CTGCTGCTGC TGCTGTGCCC TGCGCAGGTG 
    TTTTCGCAAT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CGACTGGAGA TAAGAGGTAC
    AACTGTAAAT ATTCTGGCCC ACCAAAACCC CTCCCAAAGG ACGGCTATGA CTTAGTGCAG
    GAACTCTGTC CAGGACTCTT CTTTGACAAT GTCAGTCTCT GCTGTGACAT TCAACAGCTT
    CAGACGCTGA AGAGTAACCT GCAGCTGCCC CTGCAGTTCC TGTCCAGGTG TCCGTCATGT
    TTTTATAACC TAATGACCCT GTTTTGTGAG CTAACATGTA GCCCACACCA GAGTCAGTTT
    CTGAATGTGA CAGCAACTGA GGATTATTTT GATCCTAAGA CACAGGAGAA TAAAACAAAT
    GTAAAGGAAT TAGAGTACTT TGTCGGACAG AGCTTCGCGA ATGCGATGTA CAATGCCTGC
    CGTGATGTGG AGGCGCCTTC CAGTAACGAG AAGGCCTTAG GACTCTTGTG TGGGAGGGAT
    GCCCGTGCCT GCAATGCCAC CAACTGGATT GAGTACATGT TCAATAAAGA CAACGGACAA
    GCGCCATTTA CCATCATTCC TGTGTTTTCA GATCTTTCAA TCCTTGGGAT GGAGCCCATG
    AGAAATGCCA CCAAAGGCTG CAATGAGTCT GTAGATGAGG TCACGGGGCC ATGTAGCTGC
    CAGGACTGCT CCATCGTCTG CGGCCCCAAG CCCCAGCCCC CACCCCCTCC TATGCCCTGG
    AGGATCTGGG GCTTGGATGC CATGTATGTC ATCATGTGGG TCACCTACGT GGCATTTCTG
    TTTGTGTTTT TTGGAGCACT GTTGGCAGTG TGGTGCCACA GAAGGCGGTA CTTTGTGTCT
    GAGTACACTC CCATTGACAG TAACATCGCC TTTTCTGTGA ATAGCAGTGA CAAAGGGGAA
    GCCTCATGCT GTGACCCACT TGGTGCAGCA TTTGATGACT GTCTGAGGCG CATGTTCACA
    AAGTGGGGGG CTTTCTGTGT CCGAAATCCC ACCTGCATCA TTTTCTTCTC ATTGGCCTTC
    ATCACTGTGT GCTCTTCTGG CCTGGTATTT GTCCAGGTCA CCACCAATCC TGTAGAGCTC
    TGGTCAGCCC CTCACAGTCA GGCCCGCTTG GAAAAGGAGT ACTTTGACAA GCACTTTGGG
    CCTTTCTTTC GCACGGAGCA GCTTATCATC CAGGCCCCCA ACACCAGTGT TCATATCTAC
    GAACCGTACC CCGCAGGAGC CGATGTGCCC TTCGGGCCTC CATTGAACAA AGAGATTCTG
    CACCAGGTTC TGGACTTACA GATCGCCATT GAAAGCATCA CCGCATCTTA CAACAATGAA
    ACCGTGACAC TGCAGGACAT CTGTGTGGCC CCCCTCTCTC CATACAACAA GAACTGCACC
    ATTATGAGTG TGTTAAATTA CTTCCAGAAC AGCCATGCGG TGCTGGACAG CCAAGTAGGC
    GACGACTTCT ATATCTACGC TGATTACCAC ACACACTTTC TGTACTGTGT ACGGGCTCCC
    GCCTCCTTGA ATGATACGAG TTTGCTCCAC GGTCCTTGCC TGGGTACATT TGGAGGACCG
    GTGTTCCCGT GGCTTGTGTT GGGTGGCTAT GATGATCAGA ACTACAACAA TGCCACCGCG
    CTTGTGATCA CCTTCCCCGT GAATAATTAC TACAATGACA CAGAGAGGCT CCAGAGGGCC
    TGGGCCTGGG AGAAAGAGTT TATTAGTTTT GTGAAAAACT ACAAGAATCC AAATCTGACC
    ATTTCTTTCA CTGCTGAGCG AAGCATCGAA GATGAGCTCA ATCGGGAAAG TAACAGTGAC
    GTGTTCACCG TCATCATCAG CTACGTCGTG ATGTTTCTGT ACATTTCCCT CGCCCTGGGT
    CACATCCAGA GCTGCAGCAG GCTCCTGGTG GATTCTAAGA TCTCGCTGGG CATTGCGGGG
    ATCCTGATCG TGCTAAGCTC GGTGGCCTGC TCTCTGGGCA TCTTCAGCTA CATGGGGATG
    CCGCTGACCC TCATCGTCAT TGAGGTCATC CCATTCCTGG TGCTGGCTGT CGGGGTGGAC
    AACATCTTCA TTCTAGTGCA GACCTACCAG AGAGATGAGC GTCTTCAGGA GGAAACGCTG
    GATCAGCAGC TGGGCAGGAT CCTTGGAGAA GTGGCCCCGA CCATGTTCCT TTCATCCTTT
    TCTGAGACCT CAGCATTTTT CTTTGGGGCG CTGTCCTCGA TGCCAGCTGT GCACACCTTC
    TCTCTGTTTG CGGGAATGGC CGTCCTCATT GACTTCCTCC TTCAGATTAC CTGCTTTGTG
    AGCCTGTTGG GGTTAGATAT TAAGAGGCAA GAGAAAAACC ATCTGGACAT CCTGTGCTGT
    GTCAGAGGCG CTGACGACGG ACAAGGTAGC CACGCCTCCG AAAGCTACCT GTTTCGCTTC
    TTCAAAAACT ACTTTGCCCC CCTTCTGCTG AAGGACTGGC TGAGGCCAAT TGTGGTAGCG
    GTGTTTGTGG GCGTTCTGTC ATTCAGTGTT GCGGTGGTGA ACAAAGTAGA CATCGGGTTG
    GATCAGTCTC TCTCAATGCC AAACGATTCG TATGTGATTG ACTATTTCAA ATCACTCGCT
    CAGTACCTGC ACTCGGGCCC ACCCGTGTAC TTTGTCCTGG AGGAAGGCTA TAACTACAGT
    TCACGCAAAG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GTGACAATGA CTCCCTGGTG
    CAGCAGATAT TTAACGCAGC TGAGCTGGAC ACCTACACCC GAGTAGGCTT CGCCCCCTCG
    TCCTGGATCG ATGACTACTT TGACTGGGTC TCGCCACAGT CGTCCTGCTG CAGACTCTAC
    AACGTCACTC ACCAGTTCTG CAATGCTTCT GTGATGGACC CAACCTGTGT CCGCTGCAGA
    CCTCTGACTC CAGAGGGTAA ACAGAGGCCT CAGGGGAAAG AATTCATGAA ATTCCTGCCC
    ATGTTCCTTT CTGATAACCC CAACCCCAAG TGCGGCAAAG GGGGACATGC TGCTTACGGT
    TCAGCTGTTA ACATTGTGGG AGATGACACT TACATTGGGG CCACTTACTT CATGACCTAC
    CACACCATAC TTAAGACCTC CGCTGACTAT ACTGATGCCA TGAAGAAAGC TCGGCTAATA
    GCCAGTAACA TCACGGAAAC CATGCGTTCT AAGGGGAGTG ACTACCGCGT ATTCCCTTAC
    AGTGTGTTCT ACGTCTTCTA TGAACAGTAC CTGACCATTA TTGATGACAC CATCTTTAAC
    CTCAGTGTGT CTCTGGGCTC CATATTTCTG GTGACCTTGG TGGTTCTGGG CTGTGAGCTG
    TGGTCTGCGG TCATCATGTG TATCACCATA GCCATGATCC TGGTCAACAT GTTCGGTGTC
    ATGTGGCTGT GGGGCATCAG TCTGAATGCG GTCTCCTTGG TCAACTTGGT GATGAGCTGC
    GGCATTTCTG TGGAGTTCTG CAGCCATATA ACGAGAGCAT TCACCATGAG TACCAAAGGA
    AGCCGAGTGA GCCGGGCGGA AGAGGCACTG GCCCACATGG GTAGTTCTGT ATTCAGTGGA
    ATCACACTTA CGAAATTTGG AGGGATCGTG GTGTTAGCCT TTGCCAAATC TCAAATTTTT
    GAGATATTTT ACTTCAGGAT GTACTTAGCC ATGGTCTTAC TCGGAGCCAC TCATGGACTA
    ATATTTCTTC CCGTCTTACT CAGTTACATA GGGCCGTCGG TGAATAAAGC TAAAAGACAC
    ACCACTTACG AGCGCTACAG AGGGACAGAG AGAGAACGGC TCCTCAATTT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.