Npas2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npas2
Entrez Gene ID	18143
Full Name	neuronal PAS domain protein 2
Synonyms	MOP4,bHLHe9
General protein information	Preferred Names neuronal PAS domain protein 2 Names neuronal PAS domain-containing protein 2 neuronal PAS2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 B\|1 17.98 cM
Summary	The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. The encoded protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq, Dec 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008719.2	NP_032745.2	neuronal PAS domain-containing protein 2
XM_011238452.3	XP_011236754.1	neuronal PAS domain-containing protein 2 isoform X1
XM_011238452.2	XP_011236754.1	neuronal PAS domain-containing protein 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NPAS2	NP_002509.2
	NPAS2	XP_001105763.2
Canis lupus familiaris (dog)	NPAS2	XP_005626061.1
Xenopus tropicalis (tropical clawed frog)	npas2	XP_004911838.1
Pan troglodytes (chimpanzee)	NPAS2	XP_003309214.1
Mus musculus (house mouse)	Npas2	NP_032745.2
Rattus norvegicus (Norway rat)	Npas2	NP_001101684.2
Bos taurus (cattle)	NPAS2	NP_001077232.1
Gallus gallus (chicken)	NPAS2	NP_001025713.1

Gene Ontology
Bibliography

Related articles in PubMed

Conditional postnatal deletion of the neonatal murine hepatic circadian gene, Npas2, alters the gut microbiome following restricted feeding.
O'Neil DS, Stewart CJ, Chu DM, Goodspeed DM, Gonzalez-Rodriguez PJ, Shope CD, Aagaard KM
American journal of obstetrics and gynecology217(2)218.e1-218.e15(2017 08)

Titanium biomaterials with complex surfaces induced aberrant peripheral circadian rhythms in bone marrow mesenchymal stromal cells.
Hassan N, McCarville K, Morinaga K, Mengatto CM, Langfelder P, Hokugo A, Tahara Y, Colwell CS, Nishimura I
PloS one12(8)e0183359(2017)

NPAS2 Regulation of Anxiety-Like Behavior and GABAA Receptors.
Ozburn AR, Kern J, Parekh PK, Logan RW, Liu Z, Falcon E, Becker-Krail D, Purohit K, Edgar NM, Huang Y, McClung CA
Frontiers in molecular neuroscience10360(2017)

NPAS2 Compensates for Loss of CLOCK in Peripheral Circadian Oscillators.
Landgraf D, Wang LL, Diemer T, Welsh DK
PLoS genetics12(2)e1005882(2016 Feb)

Chronic Alcohol Exposure and the Circadian Clock Mutation Exert Tissue-Specific Effects on Gene Expression in Mouse Hippocampus, Liver, and Proximal Colon.
Summa KC, Jiang P, Fitzpatrick K, Voigt RM, Bowers SJ, Forsyth CB, Vitaterna MH, Keshavarzian A, Turek FW
Alcoholism, clinical and experimental research39(10)1917-29(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we have provided an initial key insight into the interplay between neonatal establishment of the peripheral circadian clock in the liver and the ability of the gut microbiome to respond to dietary and metabolic stress.
Title: Conditional postnatal deletion of the neonatal murine hepatic circadian gene, Npas2, alters the gut microbiome following restricted feeding.

In CLOCK-deficient fibroblasts, knockdown of Npas2 leads to arrhythmicity, suggesting that NPAS2 can compensate for loss of CLOCK in peripheral cells as well as in suprachiasmatic nucleus.
Title: NPAS2 Compensates for Loss of CLOCK in Peripheral Circadian Oscillators.

Study identified a unique role for Npas2 in the regulation of cocaine reward and dopamine Drd3 receptor expression
Title: Direct regulation of diurnal Drd3 expression and cocaine reward by NPAS2.

Shp and Npas2 crosstalk is essential to maintain hepatic lipid homeostasis.
Title: Small heterodimer partner/neuronal PAS domain protein 2 axis regulates the oscillation of liver lipid metabolism.

In the absence of NADPH, a change in pH from 6.5 to 8.0 decreased the KD(app) value of the E-box from 125 to 22 nM, with an 8-fold increase in the maximal level of DNA binding for the NPAS2/BMAL1 heterodimer.
Title: Changes in pH and NADPH regulate the DNA binding activity of neuronal PAS domain protein 2, a mammalian circadian transcription factor.

The following Npas2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npas2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20478	NM_008719.2 Latest version!	Mus musculus neuronal PAS domain protein 2 (Npas2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu20478	XM_011238452.3 Latest version!	Mus musculus neuronal PAS domain protein 2 (Npas2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu20478	XM_011238452.2	Mus musculus neuronal PAS domain protein 2 (Npas2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npas2 ( NM_008719.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032745.2 Mus musculus neuronal PAS domain protein 2 (Npas2), mRNA.

Price & Availability↑

CloneID	OMu20478
Clone ID Related Accession (Same CDS sequence)	NM_008719.2 , XM_011238452.3 , XM_011238452.2
Accession Version	NM_008719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	neuronal PAS domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC109166.2, U77969.1, BB524729.2 and BG070037.2. On Dec 4, 2007 this sequence version replaced NM_008719.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109166.2, U77969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAAG ATGAGAAGGA TAGAGCAAAG AGAGCCTCTC GAAATAAGTC TGAGAAGAAG 
CGTCGGGACC AGTTCAATGT TCTCATCAAA GAGCTCAGCT CCATGCTCCC TGGTAACACT 
CGGAAAATGG ACAAAACCAC CGTGCTGGAG AAGGTCATCG GATTCTTGCA GAAACACAAT 
GAAGTCTCAG CACAAACAGA AATCTGTGAC ATCCAGCAGG ACTGGAAGCC ATCATTCCTC 
AGTAACGAAG AATTCACCCA GCTGATGTTG GAGGCATTAG ATGGCTTCGT CATCGTCGTG 
ACAACAGACG GCAGCATCAT CTATGTGTCC GACAGTATCA CACCTCTCCT TGGACATTTA 
CCGGCGGATG TCATGGATCA GAACTTGTTA AATTTCCTTC CAGAGCAAGA ACATTCCGAA 
GTTTATAAAA TCCTTTCTTC CCATATGCTT GTGACGGATT CCCCCTCCCC AGAATTCCTA 
AAATCTGACA ACGATTTAGA GTTTTATTGC CATCTTCTCA GAGGCAGCTT GAACCCAAAG 
GAATTTCCAA CTTACGAATA TATAAAATTT GTAGGAAATT TTCGCTCTTA CAACAATGTG 
CCTAGCCCCT CCTGTAATGG CTTTGACAAC ACCCTTTCAA GACCCTGCCA TGTACCCCTA 
GGAAAGGACG TCTGCTTCAT CGCCACCGTG CGCCTGGCAA CCCCGCAGTT CTTAAAGGAA 
ATGTGTGTAG CTGACGAACC TTTAGAGGAA TTCACTTCGA GGCATAGCTT GGAATGGAAA 
TTTTTATTTC TGGATCACAG AGCTCCTCCA ATCATAGGAT ACCTGCCCTT TGAAGTACTT 
GGCACCTCAG GCTACGACTA CTACCACATT GATGACCTGG AGCTCCTGGC CAGGTGCCAC 
CAGCATCTGA TGCAGTTTGG CAAAGGGAAG TCGTGCTGTT ACCGGTTTCT AACCAAAGGG 
CAGCAGTGGA TTTGGTTGCA AACCCACTAC TACATCACCT ACCACCAATG GAACTCCAAG 
CCTGAGTTCA TCGTATGCAC ACACTCAGTG GTCAGTTACG CAGATGTTCG AGTGGAAAGG 
AGACAGGAGC TGGCTCTGGA AGACCCACCC ACAGAGGCCA TGCACCCCTC TGCAGTGAAG 
GAAAAGGACT CAAGCCTAGA GCCTCCACAG CCCTTTAATG CACTTGACAT GGGCGCCTCA 
GGTCTTCCCA GCAGCCCTTC TCCATCAGCC TCCTCAAGGA GTTCCCACAA GTCCTCACAC 
ACAGCCATGT CAGAACCCAC CTCCACTCCA ACCAAGCTGA TGGCTGAGAA CAGCACCACA 
GCTTTGCCAA GACCGGCCAC CCTACCCCAG GAGTTACCAG TGCAGGGGCT CAGCCAGGCA 
GCCACAATGC CGACTGCTCT GCATTCCTCA GCCTCCTGCG ACCTCACAAA GCAACTCCTG 
CTGCAGAGCC TGCCTCAGAC CGGCTTGCAG AGTCCACCTG CTCCAGTGAC ACAGTTTTCA 
GCACAATTCA GCATGTTCCA GACCATTAAA GACCAACTGG AGCAGAGGAC ACGGATCCTG 
CAGGCCAACA TTCGGTGGCA GCAGGAAGAG CTTCATAAGA TCCAGGAACA ACTCTGCCTG 
GTCCAGGACT CCAACGTCCA GATGTTTCTG CAGCAGCCAG CTGTGTCCCT GAGCTTCAGC 
AGTACGCAGC GACCAGCAGC TCAGCAGCAG CTGCAGCAAA GGCCCGCCGC ACCGTCTCAG 
CCCCAGCTTG TGGTCAACAC TCCACTGCAG GGGCAGATCA CATCCACCCA AGTCACAAAC 
CAGCACCTGC TCCGAGAATC GAATGTGATA TCCGCCCAGG GTCCAAAGCC AATGAGAAGT 
TCCCAGCTGC TGCCTGCCAG CGGCCGTTCA CTGAGCAGCC TGCCATCCCA GTTTAGCAGT 
ACAGCTTCCG TGCTCCCGCC TGGCCTGAGC CTCACCACGA TTGCTCCCAC CCCTCAGGAT 
GACAGCCAGT GCCAGCCCAG CCCTGACTTC GGCCATGATC GGCAGCTCAG GCTGTTGCTG 
AGCCAGCCCA TCCAGCCTAT GATGCCTGGG TCCTGCGATG CCAGGCAGCC CTCAGAAGTC 
AGCAGAACTG GACGGCAAGT CAAGTATGCA CAGAGCCAAG TGATGTTTCC AAGTCCAGAC 
TCACACCCTA CCAACAGCAG CGCATCCACC CCGGTCCTGC TCATGGGGCA GGCAGTACTC 
CATCCCAGCT TCCCTGCTTC CCGGCCATCG CCACTGCAGC CAGCACAGGC ACAGCAGCAG 
CCACCACCCT ATTTGCAGGC ACCAACATCT CTGCACAGTG AGCAGCCGGA CTCGCTCCTT 
CTCTCCACCT TCTCCCAGCA GCCGGGAACC CTGGGCTATG CAGCCACACA GTCTACACCT 
CCGCAACCCC CACGCCCCTC CCGCAGGGTC AGCCGGCTGT CAGAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032745.2
CDS	497..2947
Translation	MDEDEKDRAKRASRNKSEKKRRDQFNVLIKELSSMLPGNTRKMDKTTVLEKVIGFLQKHNEVSAQTEICDIQQDWKPSFLSNEEFTQLMLEALDGFVIVVTTDGSIIYVSDSITPLLGHLPADVMDQNLLNFLPEQEHSEVYKILSSHMLVTDSPSPEFLKSDNDLEFYCHLLRGSLNPKEFPTYEYIKFVGNFRSYNNVPSPSCNGFDNTLSRPCHVPLGKDVCFIATVRLATPQFLKEMCVADEPLEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHIDDLELLARCHQHLMQFGKGKSCCYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYADVRVERRQELALEDPPTEAMHPSAVKEKDSSLEPPQPFNALDMGASGLPSSPSPSASSRSSHKSSHTAMSEPTSTPTKLMAENSTTALPRPATLPQELPVQGLSQAATMPTALHSSASCDLTKQLLLQSLPQTGLQSPPAPVTQFSAQFSMFQTIKDQLEQRTRILQANIRWQQEELHKIQEQLCLVQDSNVQMFLQQPAVSLSFSSTQRPAAQQQLQQRPAAPSQPQLVVNTPLQGQITSTQVTNQHLLRESNVISAQGPKPMRSSQLLPASGRSLSSLPSQFSSTASVLPPGLSLTTIAPTPQDDSQCQPSPDFGHDRQLRLLLSQPIQPMMPGSCDARQPSEVSRTGRQVKYAQSQVMFPSPDSHPTNSSASTPVLLMGQAVLHPSFPASRPSPLQPAQAQQQPPPYLQAPTSLHSEQPDSLLLSTFSQQPGTLGYAATQSTPPQPPRPSRRVSRLSES

Target ORF information:

RefSeq Version	NM_008719.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuronal PAS domain protein 2 (Npas2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008719.2

ORF Insert Sequence:

ATGGACGAAG ATGAGAAGGA TAGAGCAAAG AGAGCCTCTC GAAATAAGTC TGAGAAGAAG 
CGTCGGGACC AGTTCAATGT TCTCATCAAA GAGCTCAGCT CCATGCTCCC TGGTAACACT 
CGGAAAATGG ACAAAACCAC CGTGCTGGAG AAGGTCATCG GATTCTTGCA GAAACACAAT 
GAAGTCTCAG CACAAACAGA AATCTGTGAC ATCCAGCAGG ACTGGAAGCC ATCATTCCTC 
AGTAACGAAG AATTCACCCA GCTGATGTTG GAGGCATTAG ATGGCTTCGT CATCGTCGTG 
ACAACAGACG GCAGCATCAT CTATGTGTCC GACAGTATCA CACCTCTCCT TGGACATTTA 
CCGGCGGATG TCATGGATCA GAACTTGTTA AATTTCCTTC CAGAGCAAGA ACATTCCGAA 
GTTTATAAAA TCCTTTCTTC CCATATGCTT GTGACGGATT CCCCCTCCCC AGAATTCCTA 
AAATCTGACA ACGATTTAGA GTTTTATTGC CATCTTCTCA GAGGCAGCTT GAACCCAAAG 
GAATTTCCAA CTTACGAATA TATAAAATTT GTAGGAAATT TTCGCTCTTA CAACAATGTG 
CCTAGCCCCT CCTGTAATGG CTTTGACAAC ACCCTTTCAA GACCCTGCCA TGTACCCCTA 
GGAAAGGACG TCTGCTTCAT CGCCACCGTG CGCCTGGCAA CCCCGCAGTT CTTAAAGGAA 
ATGTGTGTAG CTGACGAACC TTTAGAGGAA TTCACTTCGA GGCATAGCTT GGAATGGAAA 
TTTTTATTTC TGGATCACAG AGCTCCTCCA ATCATAGGAT ACCTGCCCTT TGAAGTACTT 
GGCACCTCAG GCTACGACTA CTACCACATT GATGACCTGG AGCTCCTGGC CAGGTGCCAC 
CAGCATCTGA TGCAGTTTGG CAAAGGGAAG TCGTGCTGTT ACCGGTTTCT AACCAAAGGG 
CAGCAGTGGA TTTGGTTGCA AACCCACTAC TACATCACCT ACCACCAATG GAACTCCAAG 
CCTGAGTTCA TCGTATGCAC ACACTCAGTG GTCAGTTACG CAGATGTTCG AGTGGAAAGG 
AGACAGGAGC TGGCTCTGGA AGACCCACCC ACAGAGGCCA TGCACCCCTC TGCAGTGAAG 
GAAAAGGACT CAAGCCTAGA GCCTCCACAG CCCTTTAATG CACTTGACAT GGGCGCCTCA 
GGTCTTCCCA GCAGCCCTTC TCCATCAGCC TCCTCAAGGA GTTCCCACAA GTCCTCACAC 
ACAGCCATGT CAGAACCCAC CTCCACTCCA ACCAAGCTGA TGGCTGAGAA CAGCACCACA 
GCTTTGCCAA GACCGGCCAC CCTACCCCAG GAGTTACCAG TGCAGGGGCT CAGCCAGGCA 
GCCACAATGC CGACTGCTCT GCATTCCTCA GCCTCCTGCG ACCTCACAAA GCAACTCCTG 
CTGCAGAGCC TGCCTCAGAC CGGCTTGCAG AGTCCACCTG CTCCAGTGAC ACAGTTTTCA 
GCACAATTCA GCATGTTCCA GACCATTAAA GACCAACTGG AGCAGAGGAC ACGGATCCTG 
CAGGCCAACA TTCGGTGGCA GCAGGAAGAG CTTCATAAGA TCCAGGAACA ACTCTGCCTG 
GTCCAGGACT CCAACGTCCA GATGTTTCTG CAGCAGCCAG CTGTGTCCCT GAGCTTCAGC 
AGTACGCAGC GACCAGCAGC TCAGCAGCAG CTGCAGCAAA GGCCCGCCGC ACCGTCTCAG 
CCCCAGCTTG TGGTCAACAC TCCACTGCAG GGGCAGATCA CATCCACCCA AGTCACAAAC 
CAGCACCTGC TCCGAGAATC GAATGTGATA TCCGCCCAGG GTCCAAAGCC AATGAGAAGT 
TCCCAGCTGC TGCCTGCCAG CGGCCGTTCA CTGAGCAGCC TGCCATCCCA GTTTAGCAGT 
ACAGCTTCCG TGCTCCCGCC TGGCCTGAGC CTCACCACGA TTGCTCCCAC CCCTCAGGAT 
GACAGCCAGT GCCAGCCCAG CCCTGACTTC GGCCATGATC GGCAGCTCAG GCTGTTGCTG 
AGCCAGCCCA TCCAGCCTAT GATGCCTGGG TCCTGCGATG CCAGGCAGCC CTCAGAAGTC 
AGCAGAACTG GACGGCAAGT CAAGTATGCA CAGAGCCAAG TGATGTTTCC AAGTCCAGAC 
TCACACCCTA CCAACAGCAG CGCATCCACC CCGGTCCTGC TCATGGGGCA GGCAGTACTC 
CATCCCAGCT TCCCTGCTTC CCGGCCATCG CCACTGCAGC CAGCACAGGC ACAGCAGCAG 
CCACCACCCT ATTTGCAGGC ACCAACATCT CTGCACAGTG AGCAGCCGGA CTCGCTCCTT 
CTCTCCACCT TCTCCCAGCA GCCGGGAACC CTGGGCTATG CAGCCACACA GTCTACACCT 
CCGCAACCCC CACGCCCCTC CCGCAGGGTC AGCCGGCTGT CAGAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npas2 ( XM_011238452.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236754.1 Mus musculus neuronal PAS domain protein 2 (Npas2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20478
Clone ID Related Accession (Same CDS sequence)	NM_008719.2 , XM_011238452.3 , XM_011238452.2
Accession Version	XM_011238452.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuronal PAS domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAG ATGAGAAGGA TAGAGCAAAG AGAGCCTCTC GAAATAAGTC TGAGAAGAAG 
CGTCGGGACC AGTTCAATGT TCTCATCAAA GAGCTCAGCT CCATGCTCCC TGGTAACACT 
CGGAAAATGG ACAAAACCAC CGTGCTGGAG AAGGTCATCG GATTCTTGCA GAAACACAAT 
GAAGTCTCAG CACAAACAGA AATCTGTGAC ATCCAGCAGG ACTGGAAGCC ATCATTCCTC 
AGTAACGAAG AATTCACCCA GCTGATGTTG GAGGCATTAG ATGGCTTCGT CATCGTCGTG 
ACAACAGACG GCAGCATCAT CTATGTGTCC GACAGTATCA CACCTCTCCT TGGACATTTA 
CCGGCGGATG TCATGGATCA GAACTTGTTA AATTTCCTTC CAGAGCAAGA ACATTCCGAA 
GTTTATAAAA TCCTTTCTTC CCATATGCTT GTGACGGATT CCCCCTCCCC AGAATTCCTA 
AAATCTGACA ACGATTTAGA GTTTTATTGC CATCTTCTCA GAGGCAGCTT GAACCCAAAG 
GAATTTCCAA CTTACGAATA TATAAAATTT GTAGGAAATT TTCGCTCTTA CAACAATGTG 
CCTAGCCCCT CCTGTAATGG CTTTGACAAC ACCCTTTCAA GACCCTGCCA TGTACCCCTA 
GGAAAGGACG TCTGCTTCAT CGCCACCGTG CGCCTGGCAA CCCCGCAGTT CTTAAAGGAA 
ATGTGTGTAG CTGACGAACC TTTAGAGGAA TTCACTTCGA GGCATAGCTT GGAATGGAAA 
TTTTTATTTC TGGATCACAG AGCTCCTCCA ATCATAGGAT ACCTGCCCTT TGAAGTACTT 
GGCACCTCAG GCTACGACTA CTACCACATT GATGACCTGG AGCTCCTGGC CAGGTGCCAC 
CAGCATCTGA TGCAGTTTGG CAAAGGGAAG TCGTGCTGTT ACCGGTTTCT AACCAAAGGG 
CAGCAGTGGA TTTGGTTGCA AACCCACTAC TACATCACCT ACCACCAATG GAACTCCAAG 
CCTGAGTTCA TCGTATGCAC ACACTCAGTG GTCAGTTACG CAGATGTTCG AGTGGAAAGG 
AGACAGGAGC TGGCTCTGGA AGACCCACCC ACAGAGGCCA TGCACCCCTC TGCAGTGAAG 
GAAAAGGACT CAAGCCTAGA GCCTCCACAG CCCTTTAATG CACTTGACAT GGGCGCCTCA 
GGTCTTCCCA GCAGCCCTTC TCCATCAGCC TCCTCAAGGA GTTCCCACAA GTCCTCACAC 
ACAGCCATGT CAGAACCCAC CTCCACTCCA ACCAAGCTGA TGGCTGAGAA CAGCACCACA 
GCTTTGCCAA GACCGGCCAC CCTACCCCAG GAGTTACCAG TGCAGGGGCT CAGCCAGGCA 
GCCACAATGC CGACTGCTCT GCATTCCTCA GCCTCCTGCG ACCTCACAAA GCAACTCCTG 
CTGCAGAGCC TGCCTCAGAC CGGCTTGCAG AGTCCACCTG CTCCAGTGAC ACAGTTTTCA 
GCACAATTCA GCATGTTCCA GACCATTAAA GACCAACTGG AGCAGAGGAC ACGGATCCTG 
CAGGCCAACA TTCGGTGGCA GCAGGAAGAG CTTCATAAGA TCCAGGAACA ACTCTGCCTG 
GTCCAGGACT CCAACGTCCA GATGTTTCTG CAGCAGCCAG CTGTGTCCCT GAGCTTCAGC 
AGTACGCAGC GACCAGCAGC TCAGCAGCAG CTGCAGCAAA GGCCCGCCGC ACCGTCTCAG 
CCCCAGCTTG TGGTCAACAC TCCACTGCAG GGGCAGATCA CATCCACCCA AGTCACAAAC 
CAGCACCTGC TCCGAGAATC GAATGTGATA TCCGCCCAGG GTCCAAAGCC AATGAGAAGT 
TCCCAGCTGC TGCCTGCCAG CGGCCGTTCA CTGAGCAGCC TGCCATCCCA GTTTAGCAGT 
ACAGCTTCCG TGCTCCCGCC TGGCCTGAGC CTCACCACGA TTGCTCCCAC CCCTCAGGAT 
GACAGCCAGT GCCAGCCCAG CCCTGACTTC GGCCATGATC GGCAGCTCAG GCTGTTGCTG 
AGCCAGCCCA TCCAGCCTAT GATGCCTGGG TCCTGCGATG CCAGGCAGCC CTCAGAAGTC 
AGCAGAACTG GACGGCAAGT CAAGTATGCA CAGAGCCAAG TGATGTTTCC AAGTCCAGAC 
TCACACCCTA CCAACAGCAG CGCATCCACC CCGGTCCTGC TCATGGGGCA GGCAGTACTC 
CATCCCAGCT TCCCTGCTTC CCGGCCATCG CCACTGCAGC CAGCACAGGC ACAGCAGCAG 
CCACCACCCT ATTTGCAGGC ACCAACATCT CTGCACAGTG AGCAGCCGGA CTCGCTCCTT 
CTCTCCACCT TCTCCCAGCA GCCGGGAACC CTGGGCTATG CAGCCACACA GTCTACACCT 
CCGCAACCCC CACGCCCCTC CCGCAGGGTC AGCCGGCTGT CAGAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236754.1
CDS	321..2771
Translation	MDEDEKDRAKRASRNKSEKKRRDQFNVLIKELSSMLPGNTRKMDKTTVLEKVIGFLQKHNEVSAQTEICDIQQDWKPSFLSNEEFTQLMLEALDGFVIVVTTDGSIIYVSDSITPLLGHLPADVMDQNLLNFLPEQEHSEVYKILSSHMLVTDSPSPEFLKSDNDLEFYCHLLRGSLNPKEFPTYEYIKFVGNFRSYNNVPSPSCNGFDNTLSRPCHVPLGKDVCFIATVRLATPQFLKEMCVADEPLEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHIDDLELLARCHQHLMQFGKGKSCCYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYADVRVERRQELALEDPPTEAMHPSAVKEKDSSLEPPQPFNALDMGASGLPSSPSPSASSRSSHKSSHTAMSEPTSTPTKLMAENSTTALPRPATLPQELPVQGLSQAATMPTALHSSASCDLTKQLLLQSLPQTGLQSPPAPVTQFSAQFSMFQTIKDQLEQRTRILQANIRWQQEELHKIQEQLCLVQDSNVQMFLQQPAVSLSFSSTQRPAAQQQLQQRPAAPSQPQLVVNTPLQGQITSTQVTNQHLLRESNVISAQGPKPMRSSQLLPASGRSLSSLPSQFSSTASVLPPGLSLTTIAPTPQDDSQCQPSPDFGHDRQLRLLLSQPIQPMMPGSCDARQPSEVSRTGRQVKYAQSQVMFPSPDSHPTNSSASTPVLLMGQAVLHPSFPASRPSPLQPAQAQQQPPPYLQAPTSLHSEQPDSLLLSTFSQQPGTLGYAATQSTPPQPPRPSRRVSRLSES

Target ORF information:

RefSeq Version	XM_011238452.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuronal PAS domain protein 2 (Npas2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238452.2

ORF Insert Sequence:

ATGGACGAAG ATGAGAAGGA TAGAGCAAAG AGAGCCTCTC GAAATAAGTC TGAGAAGAAG 
CGTCGGGACC AGTTCAATGT TCTCATCAAA GAGCTCAGCT CCATGCTCCC TGGTAACACT 
CGGAAAATGG ACAAAACCAC CGTGCTGGAG AAGGTCATCG GATTCTTGCA GAAACACAAT 
GAAGTCTCAG CACAAACAGA AATCTGTGAC ATCCAGCAGG ACTGGAAGCC ATCATTCCTC 
AGTAACGAAG AATTCACCCA GCTGATGTTG GAGGCATTAG ATGGCTTCGT CATCGTCGTG 
ACAACAGACG GCAGCATCAT CTATGTGTCC GACAGTATCA CACCTCTCCT TGGACATTTA 
CCGGCGGATG TCATGGATCA GAACTTGTTA AATTTCCTTC CAGAGCAAGA ACATTCCGAA 
GTTTATAAAA TCCTTTCTTC CCATATGCTT GTGACGGATT CCCCCTCCCC AGAATTCCTA 
AAATCTGACA ACGATTTAGA GTTTTATTGC CATCTTCTCA GAGGCAGCTT GAACCCAAAG 
GAATTTCCAA CTTACGAATA TATAAAATTT GTAGGAAATT TTCGCTCTTA CAACAATGTG 
CCTAGCCCCT CCTGTAATGG CTTTGACAAC ACCCTTTCAA GACCCTGCCA TGTACCCCTA 
GGAAAGGACG TCTGCTTCAT CGCCACCGTG CGCCTGGCAA CCCCGCAGTT CTTAAAGGAA 
ATGTGTGTAG CTGACGAACC TTTAGAGGAA TTCACTTCGA GGCATAGCTT GGAATGGAAA 
TTTTTATTTC TGGATCACAG AGCTCCTCCA ATCATAGGAT ACCTGCCCTT TGAAGTACTT 
GGCACCTCAG GCTACGACTA CTACCACATT GATGACCTGG AGCTCCTGGC CAGGTGCCAC 
CAGCATCTGA TGCAGTTTGG CAAAGGGAAG TCGTGCTGTT ACCGGTTTCT AACCAAAGGG 
CAGCAGTGGA TTTGGTTGCA AACCCACTAC TACATCACCT ACCACCAATG GAACTCCAAG 
CCTGAGTTCA TCGTATGCAC ACACTCAGTG GTCAGTTACG CAGATGTTCG AGTGGAAAGG 
AGACAGGAGC TGGCTCTGGA AGACCCACCC ACAGAGGCCA TGCACCCCTC TGCAGTGAAG 
GAAAAGGACT CAAGCCTAGA GCCTCCACAG CCCTTTAATG CACTTGACAT GGGCGCCTCA 
GGTCTTCCCA GCAGCCCTTC TCCATCAGCC TCCTCAAGGA GTTCCCACAA GTCCTCACAC 
ACAGCCATGT CAGAACCCAC CTCCACTCCA ACCAAGCTGA TGGCTGAGAA CAGCACCACA 
GCTTTGCCAA GACCGGCCAC CCTACCCCAG GAGTTACCAG TGCAGGGGCT CAGCCAGGCA 
GCCACAATGC CGACTGCTCT GCATTCCTCA GCCTCCTGCG ACCTCACAAA GCAACTCCTG 
CTGCAGAGCC TGCCTCAGAC CGGCTTGCAG AGTCCACCTG CTCCAGTGAC ACAGTTTTCA 
GCACAATTCA GCATGTTCCA GACCATTAAA GACCAACTGG AGCAGAGGAC ACGGATCCTG 
CAGGCCAACA TTCGGTGGCA GCAGGAAGAG CTTCATAAGA TCCAGGAACA ACTCTGCCTG 
GTCCAGGACT CCAACGTCCA GATGTTTCTG CAGCAGCCAG CTGTGTCCCT GAGCTTCAGC 
AGTACGCAGC GACCAGCAGC TCAGCAGCAG CTGCAGCAAA GGCCCGCCGC ACCGTCTCAG 
CCCCAGCTTG TGGTCAACAC TCCACTGCAG GGGCAGATCA CATCCACCCA AGTCACAAAC 
CAGCACCTGC TCCGAGAATC GAATGTGATA TCCGCCCAGG GTCCAAAGCC AATGAGAAGT 
TCCCAGCTGC TGCCTGCCAG CGGCCGTTCA CTGAGCAGCC TGCCATCCCA GTTTAGCAGT 
ACAGCTTCCG TGCTCCCGCC TGGCCTGAGC CTCACCACGA TTGCTCCCAC CCCTCAGGAT 
GACAGCCAGT GCCAGCCCAG CCCTGACTTC GGCCATGATC GGCAGCTCAG GCTGTTGCTG 
AGCCAGCCCA TCCAGCCTAT GATGCCTGGG TCCTGCGATG CCAGGCAGCC CTCAGAAGTC 
AGCAGAACTG GACGGCAAGT CAAGTATGCA CAGAGCCAAG TGATGTTTCC AAGTCCAGAC 
TCACACCCTA CCAACAGCAG CGCATCCACC CCGGTCCTGC TCATGGGGCA GGCAGTACTC 
CATCCCAGCT TCCCTGCTTC CCGGCCATCG CCACTGCAGC CAGCACAGGC ACAGCAGCAG 
CCACCACCCT ATTTGCAGGC ACCAACATCT CTGCACAGTG AGCAGCCGGA CTCGCTCCTT 
CTCTCCACCT TCTCCCAGCA GCCGGGAACC CTGGGCTATG CAGCCACACA GTCTACACCT 
CCGCAACCCC CACGCCCCTC CCGCAGGGTC AGCCGGCTGT CAGAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npas2 ( XM_011238452.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236754.1 Mus musculus neuronal PAS domain protein 2 (Npas2), transcript variant X1, mRNA.

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CloneID	OMu20478
Clone ID Related Accession (Same CDS sequence)	NM_008719.2 , XM_011238452.3 , XM_011238452.2
Accession Version	XM_011238452.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuronal PAS domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAG ATGAGAAGGA TAGAGCAAAG AGAGCCTCTC GAAATAAGTC TGAGAAGAAG 
CGTCGGGACC AGTTCAATGT TCTCATCAAA GAGCTCAGCT CCATGCTCCC TGGTAACACT 
CGGAAAATGG ACAAAACCAC CGTGCTGGAG AAGGTCATCG GATTCTTGCA GAAACACAAT 
GAAGTCTCAG CACAAACAGA AATCTGTGAC ATCCAGCAGG ACTGGAAGCC ATCATTCCTC 
AGTAACGAAG AATTCACCCA GCTGATGTTG GAGGCATTAG ATGGCTTCGT CATCGTCGTG 
ACAACAGACG GCAGCATCAT CTATGTGTCC GACAGTATCA CACCTCTCCT TGGACATTTA 
CCGGCGGATG TCATGGATCA GAACTTGTTA AATTTCCTTC CAGAGCAAGA ACATTCCGAA 
GTTTATAAAA TCCTTTCTTC CCATATGCTT GTGACGGATT CCCCCTCCCC AGAATTCCTA 
AAATCTGACA ACGATTTAGA GTTTTATTGC CATCTTCTCA GAGGCAGCTT GAACCCAAAG 
GAATTTCCAA CTTACGAATA TATAAAATTT GTAGGAAATT TTCGCTCTTA CAACAATGTG 
CCTAGCCCCT CCTGTAATGG CTTTGACAAC ACCCTTTCAA GACCCTGCCA TGTACCCCTA 
GGAAAGGACG TCTGCTTCAT CGCCACCGTG CGCCTGGCAA CCCCGCAGTT CTTAAAGGAA 
ATGTGTGTAG CTGACGAACC TTTAGAGGAA TTCACTTCGA GGCATAGCTT GGAATGGAAA 
TTTTTATTTC TGGATCACAG AGCTCCTCCA ATCATAGGAT ACCTGCCCTT TGAAGTACTT 
GGCACCTCAG GCTACGACTA CTACCACATT GATGACCTGG AGCTCCTGGC CAGGTGCCAC 
CAGCATCTGA TGCAGTTTGG CAAAGGGAAG TCGTGCTGTT ACCGGTTTCT AACCAAAGGG 
CAGCAGTGGA TTTGGTTGCA AACCCACTAC TACATCACCT ACCACCAATG GAACTCCAAG 
CCTGAGTTCA TCGTATGCAC ACACTCAGTG GTCAGTTACG CAGATGTTCG AGTGGAAAGG 
AGACAGGAGC TGGCTCTGGA AGACCCACCC ACAGAGGCCA TGCACCCCTC TGCAGTGAAG 
GAAAAGGACT CAAGCCTAGA GCCTCCACAG CCCTTTAATG CACTTGACAT GGGCGCCTCA 
GGTCTTCCCA GCAGCCCTTC TCCATCAGCC TCCTCAAGGA GTTCCCACAA GTCCTCACAC 
ACAGCCATGT CAGAACCCAC CTCCACTCCA ACCAAGCTGA TGGCTGAGAA CAGCACCACA 
GCTTTGCCAA GACCGGCCAC CCTACCCCAG GAGTTACCAG TGCAGGGGCT CAGCCAGGCA 
GCCACAATGC CGACTGCTCT GCATTCCTCA GCCTCCTGCG ACCTCACAAA GCAACTCCTG 
CTGCAGAGCC TGCCTCAGAC CGGCTTGCAG AGTCCACCTG CTCCAGTGAC ACAGTTTTCA 
GCACAATTCA GCATGTTCCA GACCATTAAA GACCAACTGG AGCAGAGGAC ACGGATCCTG 
CAGGCCAACA TTCGGTGGCA GCAGGAAGAG CTTCATAAGA TCCAGGAACA ACTCTGCCTG 
GTCCAGGACT CCAACGTCCA GATGTTTCTG CAGCAGCCAG CTGTGTCCCT GAGCTTCAGC 
AGTACGCAGC GACCAGCAGC TCAGCAGCAG CTGCAGCAAA GGCCCGCCGC ACCGTCTCAG 
CCCCAGCTTG TGGTCAACAC TCCACTGCAG GGGCAGATCA CATCCACCCA AGTCACAAAC 
CAGCACCTGC TCCGAGAATC GAATGTGATA TCCGCCCAGG GTCCAAAGCC AATGAGAAGT 
TCCCAGCTGC TGCCTGCCAG CGGCCGTTCA CTGAGCAGCC TGCCATCCCA GTTTAGCAGT 
ACAGCTTCCG TGCTCCCGCC TGGCCTGAGC CTCACCACGA TTGCTCCCAC CCCTCAGGAT 
GACAGCCAGT GCCAGCCCAG CCCTGACTTC GGCCATGATC GGCAGCTCAG GCTGTTGCTG 
AGCCAGCCCA TCCAGCCTAT GATGCCTGGG TCCTGCGATG CCAGGCAGCC CTCAGAAGTC 
AGCAGAACTG GACGGCAAGT CAAGTATGCA CAGAGCCAAG TGATGTTTCC AAGTCCAGAC 
TCACACCCTA CCAACAGCAG CGCATCCACC CCGGTCCTGC TCATGGGGCA GGCAGTACTC 
CATCCCAGCT TCCCTGCTTC CCGGCCATCG CCACTGCAGC CAGCACAGGC ACAGCAGCAG 
CCACCACCCT ATTTGCAGGC ACCAACATCT CTGCACAGTG AGCAGCCGGA CTCGCTCCTT 
CTCTCCACCT TCTCCCAGCA GCCGGGAACC CTGGGCTATG CAGCCACACA GTCTACACCT 
CCGCAACCCC CACGCCCCTC CCGCAGGGTC AGCCGGCTGT CAGAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236754.1
CDS	334..2784
Translation	MDEDEKDRAKRASRNKSEKKRRDQFNVLIKELSSMLPGNTRKMDKTTVLEKVIGFLQKHNEVSAQTEICDIQQDWKPSFLSNEEFTQLMLEALDGFVIVVTTDGSIIYVSDSITPLLGHLPADVMDQNLLNFLPEQEHSEVYKILSSHMLVTDSPSPEFLKSDNDLEFYCHLLRGSLNPKEFPTYEYIKFVGNFRSYNNVPSPSCNGFDNTLSRPCHVPLGKDVCFIATVRLATPQFLKEMCVADEPLEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHIDDLELLARCHQHLMQFGKGKSCCYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYADVRVERRQELALEDPPTEAMHPSAVKEKDSSLEPPQPFNALDMGASGLPSSPSPSASSRSSHKSSHTAMSEPTSTPTKLMAENSTTALPRPATLPQELPVQGLSQAATMPTALHSSASCDLTKQLLLQSLPQTGLQSPPAPVTQFSAQFSMFQTIKDQLEQRTRILQANIRWQQEELHKIQEQLCLVQDSNVQMFLQQPAVSLSFSSTQRPAAQQQLQQRPAAPSQPQLVVNTPLQGQITSTQVTNQHLLRESNVISAQGPKPMRSSQLLPASGRSLSSLPSQFSSTASVLPPGLSLTTIAPTPQDDSQCQPSPDFGHDRQLRLLLSQPIQPMMPGSCDARQPSEVSRTGRQVKYAQSQVMFPSPDSHPTNSSASTPVLLMGQAVLHPSFPASRPSPLQPAQAQQQPPPYLQAPTSLHSEQPDSLLLSTFSQQPGTLGYAATQSTPPQPPRPSRRVSRLSES

Target ORF information:

RefSeq Version	XM_011238452.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuronal PAS domain protein 2 (Npas2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238452.3

ORF Insert Sequence:

ATGGACGAAG ATGAGAAGGA TAGAGCAAAG AGAGCCTCTC GAAATAAGTC TGAGAAGAAG 
CGTCGGGACC AGTTCAATGT TCTCATCAAA GAGCTCAGCT CCATGCTCCC TGGTAACACT 
CGGAAAATGG ACAAAACCAC CGTGCTGGAG AAGGTCATCG GATTCTTGCA GAAACACAAT 
GAAGTCTCAG CACAAACAGA AATCTGTGAC ATCCAGCAGG ACTGGAAGCC ATCATTCCTC 
AGTAACGAAG AATTCACCCA GCTGATGTTG GAGGCATTAG ATGGCTTCGT CATCGTCGTG 
ACAACAGACG GCAGCATCAT CTATGTGTCC GACAGTATCA CACCTCTCCT TGGACATTTA 
CCGGCGGATG TCATGGATCA GAACTTGTTA AATTTCCTTC CAGAGCAAGA ACATTCCGAA 
GTTTATAAAA TCCTTTCTTC CCATATGCTT GTGACGGATT CCCCCTCCCC AGAATTCCTA 
AAATCTGACA ACGATTTAGA GTTTTATTGC CATCTTCTCA GAGGCAGCTT GAACCCAAAG 
GAATTTCCAA CTTACGAATA TATAAAATTT GTAGGAAATT TTCGCTCTTA CAACAATGTG 
CCTAGCCCCT CCTGTAATGG CTTTGACAAC ACCCTTTCAA GACCCTGCCA TGTACCCCTA 
GGAAAGGACG TCTGCTTCAT CGCCACCGTG CGCCTGGCAA CCCCGCAGTT CTTAAAGGAA 
ATGTGTGTAG CTGACGAACC TTTAGAGGAA TTCACTTCGA GGCATAGCTT GGAATGGAAA 
TTTTTATTTC TGGATCACAG AGCTCCTCCA ATCATAGGAT ACCTGCCCTT TGAAGTACTT 
GGCACCTCAG GCTACGACTA CTACCACATT GATGACCTGG AGCTCCTGGC CAGGTGCCAC 
CAGCATCTGA TGCAGTTTGG CAAAGGGAAG TCGTGCTGTT ACCGGTTTCT AACCAAAGGG 
CAGCAGTGGA TTTGGTTGCA AACCCACTAC TACATCACCT ACCACCAATG GAACTCCAAG 
CCTGAGTTCA TCGTATGCAC ACACTCAGTG GTCAGTTACG CAGATGTTCG AGTGGAAAGG 
AGACAGGAGC TGGCTCTGGA AGACCCACCC ACAGAGGCCA TGCACCCCTC TGCAGTGAAG 
GAAAAGGACT CAAGCCTAGA GCCTCCACAG CCCTTTAATG CACTTGACAT GGGCGCCTCA 
GGTCTTCCCA GCAGCCCTTC TCCATCAGCC TCCTCAAGGA GTTCCCACAA GTCCTCACAC 
ACAGCCATGT CAGAACCCAC CTCCACTCCA ACCAAGCTGA TGGCTGAGAA CAGCACCACA 
GCTTTGCCAA GACCGGCCAC CCTACCCCAG GAGTTACCAG TGCAGGGGCT CAGCCAGGCA 
GCCACAATGC CGACTGCTCT GCATTCCTCA GCCTCCTGCG ACCTCACAAA GCAACTCCTG 
CTGCAGAGCC TGCCTCAGAC CGGCTTGCAG AGTCCACCTG CTCCAGTGAC ACAGTTTTCA 
GCACAATTCA GCATGTTCCA GACCATTAAA GACCAACTGG AGCAGAGGAC ACGGATCCTG 
CAGGCCAACA TTCGGTGGCA GCAGGAAGAG CTTCATAAGA TCCAGGAACA ACTCTGCCTG 
GTCCAGGACT CCAACGTCCA GATGTTTCTG CAGCAGCCAG CTGTGTCCCT GAGCTTCAGC 
AGTACGCAGC GACCAGCAGC TCAGCAGCAG CTGCAGCAAA GGCCCGCCGC ACCGTCTCAG 
CCCCAGCTTG TGGTCAACAC TCCACTGCAG GGGCAGATCA CATCCACCCA AGTCACAAAC 
CAGCACCTGC TCCGAGAATC GAATGTGATA TCCGCCCAGG GTCCAAAGCC AATGAGAAGT 
TCCCAGCTGC TGCCTGCCAG CGGCCGTTCA CTGAGCAGCC TGCCATCCCA GTTTAGCAGT 
ACAGCTTCCG TGCTCCCGCC TGGCCTGAGC CTCACCACGA TTGCTCCCAC CCCTCAGGAT 
GACAGCCAGT GCCAGCCCAG CCCTGACTTC GGCCATGATC GGCAGCTCAG GCTGTTGCTG 
AGCCAGCCCA TCCAGCCTAT GATGCCTGGG TCCTGCGATG CCAGGCAGCC CTCAGAAGTC 
AGCAGAACTG GACGGCAAGT CAAGTATGCA CAGAGCCAAG TGATGTTTCC AAGTCCAGAC 
TCACACCCTA CCAACAGCAG CGCATCCACC CCGGTCCTGC TCATGGGGCA GGCAGTACTC 
CATCCCAGCT TCCCTGCTTC CCGGCCATCG CCACTGCAGC CAGCACAGGC ACAGCAGCAG 
CCACCACCCT ATTTGCAGGC ACCAACATCT CTGCACAGTG AGCAGCCGGA CTCGCTCCTT 
CTCTCCACCT TCTCCCAGCA GCCGGGAACC CTGGGCTATG CAGCCACACA GTCTACACCT 
CCGCAACCCC CACGCCCCTC CCGCAGGGTC AGCCGGCTGT CAGAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conditional postnatal deletion of the neonatal murine hepatic circadian gene, Npas2, alters the gut microbiome following restricted feeding.
American journal of obstetrics and gynecology217(2)218.e1-218.e15(2017 08)
O'Neil DS,Stewart CJ,Chu DM,Goodspeed DM,Gonzalez-Rodriguez PJ,Shope CD,Aagaard KM

Titanium biomaterials with complex surfaces induced aberrant peripheral circadian rhythms in bone marrow mesenchymal stromal cells.
PloS one12(8)e0183359(2017)
Hassan N,McCarville K,Morinaga K,Mengatto CM,Langfelder P,Hokugo A,Tahara Y,Colwell CS,Nishimura I

NPAS2 Regulation of Anxiety-Like Behavior and GABAA Receptors.
Frontiers in molecular neuroscience10360(2017)
Ozburn AR,Kern J,Parekh PK,Logan RW,Liu Z,Falcon E,Becker-Krail D,Purohit K,Edgar NM,Huang Y,McClung CA

NPAS2 Compensates for Loss of CLOCK in Peripheral Circadian Oscillators.
PLoS genetics12(2)e1005882(2016 Feb)
Landgraf D,Wang LL,Diemer T,Welsh DK

Chronic Alcohol Exposure and the Circadian Clock Mutation Exert Tissue-Specific Effects on Gene Expression in Mouse Hippocampus, Liver, and Proximal Colon.
Alcoholism, clinical and experimental research39(10)1917-29(2015 Oct)
Summa KC,Jiang P,Fitzpatrick K,Voigt RM,Bowers SJ,Forsyth CB,Vitaterna MH,Keshavarzian A,Turek FW

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Npas2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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