Nos3 cDNA ORF clone, Mus musculus(house mouse)

The following Nos3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nos3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu20082 NM_008713.4
Latest version!
Mus musculus nitric oxide synthase 3, endothelial cell (Nos3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $643.30
$919.00
OMu34722 XM_006535639.4
Latest version!
Mus musculus nitric oxide synthase 3, endothelial cell (Nos3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OMu34722 XM_006535639.3 Mus musculus nitric oxide synthase 3, endothelial cell (Nos3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu20082
    Clone ID Related Accession (Same CDS sequence) NM_008713.4
    Accession Version NM_008713.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, endothelial
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077896.1, U53142.1 and AW489695.1. On Nov 27, 2007 this sequence version replaced NM_008713.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077896.1, BC052636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTGGGCCAC CCTGTGGCCT AGGGCTCGGG 
    CTGGGTTTAG GGCTGTGCGG CAAGCAGGGC CCAGCCTCTC CAGCACCGGA GCCTAGCCAG
    GCGCCAGCAC CCCCGTCCCC AACCCGACCA GCACCAGACC ACAGCCCCCC GCTAACCCGG
    CCCCCAGACG GACCCAGGTT TCCTCGAGTA AAGAATTGGG AAGTGGGCAG CATCACCTAC
    GACACCCTCA GTGCCCAGGC TCAGCAGGAT GGGCCCTGTA CCTCAAGACG CTGCTTGGGA
    TCCCTGGTGT TTCCAAGGAA GTTACAGAGC CGGCCCACCC AGGGCCCTTC ACCCACTGAG
    CAGCTATTGG GTCAAGCCCG GGACTTCATC AATCAGTACT ATAACTCCAT CAAAAGGAGT
    GGCTCCCAGG CTCATGAGCA GCGGCTTCAG GAAGTGGAGG CTGAGGTGGC AGCCACAGGC
    ACCTACCAGC TCCGGGAGAG CGAGCTGGTG TTTGGGGCCA AGCAGGCCTG GCGCAATGCT
    CCCCGCTGTG TGGGCCGGAT CCAGTGGGGA AAGCTGCAGG TATTTGATGC TCGGGACTGC
    AGGACTGCAC AGGAAATGTT CACCTACATC TGTAACCACA TTAAATACGC AACAAATAGA
    GGCAATCTTC GTTCAGCCAT CACAGTGTTC CCCCAGCGCT GCCCTGGCCG GGGAGACTTC
    CGGATCTGGA ACAGCCAGCT GATACGCTAT GCGGGCTATA GGCAGCAGGA TGGCTCCGTG
    CGAGGGGACC CCGCCAACGT GGAGATCACT GAGCTCTGTA TCCAACATGG CTGGACCCCA
    GGAAATGGCC GCTTTGATGT GCTGCCCCTG TTACTCCAGG CTCCTGATGA GCCCCCAGAA
    CTCTTCACTC TGCCCCCAGA GATGGTCCTC GAGGTGCCTC TGGAGCACCC CACGCTCGAG
    TGGTTTGCTG CCCTTGGCCT GCGCTGGTAT GCCCTCCCAG CTGTGTCCAA CATGCTGCTA
    GAAATCGGGG GCCTGGAGTT TCCTGCTGCC CCTTTCAGCG GCTGGTACAT GAGTTCAGAG
    ATTGGCATGA GGGACCTGTG TGACCCTCAC CGCTACAACA TACTTGAGGA TGTGGCTGTG
    TGCATGGATC TGGACACCAG GACAACCTCA TCCCTGTGGA AAGACAAGGC AGCGGTGGAA
    ATTAATGTGG CCGTGTTGCA CAGTTACCAG CTGGCCAAAG TGACCATAGT GGACCACCAC
    GCCGCCACAG CCTCCTTCAT GAAGCACCTG GAAAATGAGC AGAAGGCCAG AGGGGGCTGC
    CCTGCCGATT GGGCCTGGAT TGTGCCCCCC ATCTCAGGCA GCCTAACTCC TGTCTTCCAT
    CAAGAGATGG TCAACTATTT CCTGTCCCCT GCCTTCCGCT ACCAGCCAGA CCCCTGGAAG
    GGAAGTGCAG CAAAGGGGGC AGGCATCACC AGGAAGAAGA CCTTTAAGGA AGTAGCCAAT
    GCAGTGAAGA TCTCTGCCTC ACTCATGGGC ACGGTGATGG CGAAGCGTGT GAAGGCAACC
    ATTCTGTATG GCTCTGAGAC TGGCCGGGCC CAGAGCTACG CACAGCAGCT GGGAAGACTC
    TTCCGGAAGG CGTTTGATCC CCGGGTCCTG TGCATGGATG AGTATGATGT GGTGTCCCTA
    GAGCACGAGG CACTGGTGTT GGTGGTGACC AGCACATTTG GCAATGGGGA TCCTCCGGAG
    AATGGAGAGA GCTTTGCAGC AGCGCTCATG GAAATGTCAG GCCCGTACAA CAGCTCCCCT
    AGGCCTGAGC AGCACAAGAG CTACAAAATC CGATTCAACA GTGTCTCCTG CTCAGACCCA
    CTGGTATCCT CTTGGCGGCG CAAGAGGAAG GAGTCTAGCA ACACAGACAG TGCAGGAGCC
    CTGGGCACCC TCAGGTTCTG TGTGTTTGGG CTGGGCTCCC GAGCATACCC CCACTTCTGT
    GCCTTTGCTC GAGCGGTGGA CACAAGGCTG GAGGAGCTGG GCGGGGAGCG ACTACTGCAG
    CTGGGCCAAG GTGATGAGCT CTGTGGCCAG GAGGAGGCTT TCCGAGGCTG GGCCCAGGCC
    GCCTTCCAGG CTGCCTGTGA AACCTTCTGT GTGGGAGAAG ATGCCAAAGC TGCTGCCCGA
    GATATCTTCA GCCCCAAACG CAGCTGGAAG CGCCAGAGGT ACCGGCTGAG TACCCAGGCT
    GAGAGCCTGC AATTACTACC AGGGCTGACT CACGTGCACA GGCGGAAGAT GTTCCAGGCT
    ACAATCCTCT CTGTGGAAAA CCTACAGAGC AGCAAATCCA CCCGAGCCAC GATCCTGGTG
    CGTCTGGACA CCGGAGGCCA GGAGGGACTG CAGTACCAGC CAGGGGACCA CATAGGTGTG
    TGCCCACCCA ACCGTCCTGG CCTAGTGGAG GCACTGCTGA GCCGAGTGGA GGACCCTCCG
    CCATCCACAG AACCTGTGGC TGTGGAACAA CTGGAGAAAG GCAGCCCTGG TGGCCCTCCC
    CCCGGCTGGG TACGGGACCC CCGGCTACCC CCATGTACGC TGCGGCAGGC TCTCACCTAC
    TTCCTGGACA TCACTTCCCC GCCTAGTCCT CGCCTCCTTC GACTGCTCAG CACCCTGGCA
    GAAGAGTCCA GCGAACAGCA GGAGCTAGAG GCTCTCAGCC AGGACCCCCG GCGCTACGAA
    GAATGGAAGT GGTTCAGCTG CCCCACACTG CTAGAGGTGC TGGAGCAATT TCCTTCAGTG
    GCACTGCCTG CCCCGCTGAT CCTCACCCAG CTGCCCTTGC TCCAGCCCCG GTACTACTCT
    GTCAGTTCAG CACCCAGCGC CCACCCAGGA GAGATCCACC TCACCATAGC TGTGCTGGCT
    TACAGAACCC AGGATGGGCT GGGCCCTCTG CACTACGGTG TCTGCTCCAC GTGGATGAGC
    CAGCTCAAGG CGGGAGATCC AGTGCCCTGC TTCATCAGGG GGGCTCCCTC CTTCCGGCTG
    CCACCTGATC CTAACTTGCC CTGCATCCTG GTGGGCCCAG GGACTGGCAT TGCACCCTTC
    CGGGGATTCT GGCAAGACAG ACTACACGAC ATTGAGATCA AAGGGCTACA ACCTGCCCCC
    ATGACTTTGG TGTTTGGCTG CCGATGCTCC CAACTGGACC ATCTCTACCG GGACGAGGTA
    CTGGACGCCC AGCAGCGTGG GGTGTTTGGA CAAGTCCTCA CCGCCTTTTC CAGGGATCCT
    GGCAGCCCCA AGACCTACGT GCAAGACCTC CTGAGGACAG AGCTAGCCGC GGAGGTTCAC
    CGTGTGCTGT GCCTTGAGCA AGGACATATG TTTGTCTGCG GCGATGTCAC TATGGCAACC
    AGCGTCCTGC AAACCGTGCA GAGAATTCTG GCAACAGAGG GCGGCATGGA GCTGGATGAA
    GCCGGTGACG TCATCGGCGT GCTGCGGGAT CAGCAACGCT ACCACGAGGA CATTTTCGGA
    CTCACATTGC GCACCCAGGA GGTGACAAGC CGCATACGCA CCCAGAGCTT TTCTTTGCAG
    GAGCGACAGC TGAGGGGCGC AGTGCCCTGG TCCTTTGACC CGCCTGGCCC AGAAATACCT
    GGTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032739.3
    CDS20..3628
    Translation

    Target ORF information:

    RefSeq Version NM_008713.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 3, endothelial cell (Nos3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008713.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCTGGGCCAC CCTGTGGCCT AGGGCTCGGG 
    CTGGGTTTAG GGCTGTGCGG CAAGCAGGGC CCAGCCTCTC CAGCACCGGA GCCTAGCCAG
    GCGCCAGCAC CCCCGTCCCC AACCCGACCA GCACCAGACC ACAGCCCCCC GCTAACCCGG
    CCCCCAGACG GACCCAGGTT TCCTCGAGTA AAGAATTGGG AAGTGGGCAG CATCACCTAC
    GACACCCTCA GTGCCCAGGC TCAGCAGGAT GGGCCCTGTA CCTCAAGACG CTGCTTGGGA
    TCCCTGGTGT TTCCAAGGAA GTTACAGAGC CGGCCCACCC AGGGCCCTTC ACCCACTGAG
    CAGCTATTGG GTCAAGCCCG GGACTTCATC AATCAGTACT ATAACTCCAT CAAAAGGAGT
    GGCTCCCAGG CTCATGAGCA GCGGCTTCAG GAAGTGGAGG CTGAGGTGGC AGCCACAGGC
    ACCTACCAGC TCCGGGAGAG CGAGCTGGTG TTTGGGGCCA AGCAGGCCTG GCGCAATGCT
    CCCCGCTGTG TGGGCCGGAT CCAGTGGGGA AAGCTGCAGG TATTTGATGC TCGGGACTGC
    AGGACTGCAC AGGAAATGTT CACCTACATC TGTAACCACA TTAAATACGC AACAAATAGA
    GGCAATCTTC GTTCAGCCAT CACAGTGTTC CCCCAGCGCT GCCCTGGCCG GGGAGACTTC
    CGGATCTGGA ACAGCCAGCT GATACGCTAT GCGGGCTATA GGCAGCAGGA TGGCTCCGTG
    CGAGGGGACC CCGCCAACGT GGAGATCACT GAGCTCTGTA TCCAACATGG CTGGACCCCA
    GGAAATGGCC GCTTTGATGT GCTGCCCCTG TTACTCCAGG CTCCTGATGA GCCCCCAGAA
    CTCTTCACTC TGCCCCCAGA GATGGTCCTC GAGGTGCCTC TGGAGCACCC CACGCTCGAG
    TGGTTTGCTG CCCTTGGCCT GCGCTGGTAT GCCCTCCCAG CTGTGTCCAA CATGCTGCTA
    GAAATCGGGG GCCTGGAGTT TCCTGCTGCC CCTTTCAGCG GCTGGTACAT GAGTTCAGAG
    ATTGGCATGA GGGACCTGTG TGACCCTCAC CGCTACAACA TACTTGAGGA TGTGGCTGTG
    TGCATGGATC TGGACACCAG GACAACCTCA TCCCTGTGGA AAGACAAGGC AGCGGTGGAA
    ATTAATGTGG CCGTGTTGCA CAGTTACCAG CTGGCCAAAG TGACCATAGT GGACCACCAC
    GCCGCCACAG CCTCCTTCAT GAAGCACCTG GAAAATGAGC AGAAGGCCAG AGGGGGCTGC
    CCTGCCGATT GGGCCTGGAT TGTGCCCCCC ATCTCAGGCA GCCTAACTCC TGTCTTCCAT
    CAAGAGATGG TCAACTATTT CCTGTCCCCT GCCTTCCGCT ACCAGCCAGA CCCCTGGAAG
    GGAAGTGCAG CAAAGGGGGC AGGCATCACC AGGAAGAAGA CCTTTAAGGA AGTAGCCAAT
    GCAGTGAAGA TCTCTGCCTC ACTCATGGGC ACGGTGATGG CGAAGCGTGT GAAGGCAACC
    ATTCTGTATG GCTCTGAGAC TGGCCGGGCC CAGAGCTACG CACAGCAGCT GGGAAGACTC
    TTCCGGAAGG CGTTTGATCC CCGGGTCCTG TGCATGGATG AGTATGATGT GGTGTCCCTA
    GAGCACGAGG CACTGGTGTT GGTGGTGACC AGCACATTTG GCAATGGGGA TCCTCCGGAG
    AATGGAGAGA GCTTTGCAGC AGCGCTCATG GAAATGTCAG GCCCGTACAA CAGCTCCCCT
    AGGCCTGAGC AGCACAAGAG CTACAAAATC CGATTCAACA GTGTCTCCTG CTCAGACCCA
    CTGGTATCCT CTTGGCGGCG CAAGAGGAAG GAGTCTAGCA ACACAGACAG TGCAGGAGCC
    CTGGGCACCC TCAGGTTCTG TGTGTTTGGG CTGGGCTCCC GAGCATACCC CCACTTCTGT
    GCCTTTGCTC GAGCGGTGGA CACAAGGCTG GAGGAGCTGG GCGGGGAGCG ACTACTGCAG
    CTGGGCCAAG GTGATGAGCT CTGTGGCCAG GAGGAGGCTT TCCGAGGCTG GGCCCAGGCC
    GCCTTCCAGG CTGCCTGTGA AACCTTCTGT GTGGGAGAAG ATGCCAAAGC TGCTGCCCGA
    GATATCTTCA GCCCCAAACG CAGCTGGAAG CGCCAGAGGT ACCGGCTGAG TACCCAGGCT
    GAGAGCCTGC AATTACTACC AGGGCTGACT CACGTGCACA GGCGGAAGAT GTTCCAGGCT
    ACAATCCTCT CTGTGGAAAA CCTACAGAGC AGCAAATCCA CCCGAGCCAC GATCCTGGTG
    CGTCTGGACA CCGGAGGCCA GGAGGGACTG CAGTACCAGC CAGGGGACCA CATAGGTGTG
    TGCCCACCCA ACCGTCCTGG CCTAGTGGAG GCACTGCTGA GCCGAGTGGA GGACCCTCCG
    CCATCCACAG AACCTGTGGC TGTGGAACAA CTGGAGAAAG GCAGCCCTGG TGGCCCTCCC
    CCCGGCTGGG TACGGGACCC CCGGCTACCC CCATGTACGC TGCGGCAGGC TCTCACCTAC
    TTCCTGGACA TCACTTCCCC GCCTAGTCCT CGCCTCCTTC GACTGCTCAG CACCCTGGCA
    GAAGAGTCCA GCGAACAGCA GGAGCTAGAG GCTCTCAGCC AGGACCCCCG GCGCTACGAA
    GAATGGAAGT GGTTCAGCTG CCCCACACTG CTAGAGGTGC TGGAGCAATT TCCTTCAGTG
    GCACTGCCTG CCCCGCTGAT CCTCACCCAG CTGCCCTTGC TCCAGCCCCG GTACTACTCT
    GTCAGTTCAG CACCCAGCGC CCACCCAGGA GAGATCCACC TCACCATAGC TGTGCTGGCT
    TACAGAACCC AGGATGGGCT GGGCCCTCTG CACTACGGTG TCTGCTCCAC GTGGATGAGC
    CAGCTCAAGG CGGGAGATCC AGTGCCCTGC TTCATCAGGG GGGCTCCCTC CTTCCGGCTG
    CCACCTGATC CTAACTTGCC CTGCATCCTG GTGGGCCCAG GGACTGGCAT TGCACCCTTC
    CGGGGATTCT GGCAAGACAG ACTACACGAC ATTGAGATCA AAGGGCTACA ACCTGCCCCC
    ATGACTTTGG TGTTTGGCTG CCGATGCTCC CAACTGGACC ATCTCTACCG GGACGAGGTA
    CTGGACGCCC AGCAGCGTGG GGTGTTTGGA CAAGTCCTCA CCGCCTTTTC CAGGGATCCT
    GGCAGCCCCA AGACCTACGT GCAAGACCTC CTGAGGACAG AGCTAGCCGC GGAGGTTCAC
    CGTGTGCTGT GCCTTGAGCA AGGACATATG TTTGTCTGCG GCGATGTCAC TATGGCAACC
    AGCGTCCTGC AAACCGTGCA GAGAATTCTG GCAACAGAGG GCGGCATGGA GCTGGATGAA
    GCCGGTGACG TCATCGGCGT GCTGCGGGAT CAGCAACGCT ACCACGAGGA CATTTTCGGA
    CTCACATTGC GCACCCAGGA GGTGACAAGC CGCATACGCA CCCAGAGCTT TTCTTTGCAG
    GAGCGACAGC TGAGGGGCGC AGTGCCCTGG TCCTTTGACC CGCCTGGCCC AGAAATACCT
    GGTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu34722
    Clone ID Related Accession (Same CDS sequence) XM_006535639.4 , XM_006535639.3
    Accession Version XM_006535639.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, endothelial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535639.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCAGCC CCCCGCTAAC CCGGCCCCCA GACGGACCCA GGTTTCCTCG AGTAAAGAAT 
    TGGGAAGTGG GCAGCATCAC CTACGACACC CTCAGTGCCC AGGCTCAGCA GGATGGGCCC
    TGTACCTCAA GACGCTGCTT GGGATCCCTG GTGTTTCCAA GGAAGTTACA GAGCCGGCCC
    ACCCAGGGCC CTTCACCCAC TGAGCAGCTA TTGGGTCAAG CCCGGGACTT CATCAATCAG
    TACTATAACT CCATCAAAAG GAGTGGCTCC CAGGCTCATG AGCAGCGGCT TCAGGAAGTG
    GAGGCTGAGG TGGCAGCCAC AGGCACCTAC CAGCTCCGGG AGAGCGAGCT GGTGTTTGGG
    GCCAAGCAGG CCTGGCGCAA TGCTCCCCGC TGTGTGGGCC GGATCCAGTG GGGAAAGCTG
    CAGGTATTTG ATGCTCGGGA CTGCAGGACT GCACAGGAAA TGTTCACCTA CATCTGTAAC
    CACATTAAAT ACGCAACAAA TAGAGGCAAT CTTCGTTCAG CCATCACAGT GTTCCCCCAG
    CGCTGCCCTG GCCGGGGAGA CTTCCGGATC TGGAACAGCC AGCTGATACG CTATGCGGGC
    TATAGGCAGC AGGATGGCTC CGTGCGAGGG GACCCCGCCA ACGTGGAGAT CACTGAGCTC
    TGTATCCAAC ATGGCTGGAC CCCAGGAAAT GGCCGCTTTG ATGTGCTGCC CCTGTTACTC
    CAGGCTCCTG ATGAGCCCCC AGAACTCTTC ACTCTGCCCC CAGAGATGGT CCTCGAGGTG
    CCTCTGGAGC ACCCCACGCT CGAGTGGTTT GCTGCCCTTG GCCTGCGCTG GTATGCCCTC
    CCAGCTGTGT CCAACATGCT GCTAGAAATC GGGGGCCTGG AGTTTCCTGC TGCCCCTTTC
    AGCGGCTGGT ACATGAGTTC AGAGATTGGC ATGAGGGACC TGTGTGACCC TCACCGCTAC
    AACATACTTG AGGATGTGGC TGTGTGCATG GATCTGGACA CCAGGACAAC CTCATCCCTG
    TGGAAAGACA AGGCAGCGGT GGAAATTAAT GTGGCCGTGT TGCACAGTTA CCAGCTGGCC
    AAAGTGACCA TAGTGGACCA CCACGCCGCC ACAGCCTCCT TCATGAAGCA CCTGGAAAAT
    GAGCAGAAGG CCAGAGGGGG CTGCCCTGCC GATTGGGCCT GGATTGTGCC CCCCATCTCA
    GGCAGCCTAA CTCCTGTCTT CCATCAAGAG ATGGTCAACT ATTTCCTGTC CCCTGCCTTC
    CGCTACCAGC CAGACCCCTG GAAGGGAAGT GCAGCAAAGG GGGCAGGCAT CACCAGGAAG
    AAGACCTTTA AGGAAGTAGC CAATGCAGTG AAGATCTCTG CCTCACTCAT GGGCACGGTG
    ATGGCGAAGC GTGTGAAGGC AACCATTCTG TATGGCTCTG AGACTGGCCG GGCCCAGAGC
    TACGCACAGC AGCTGGGAAG ACTCTTCCGG AAGGCGTTTG ATCCCCGGGT CCTGTGCATG
    GATGAGTATG ATGTGGTGTC CCTAGAGCAC GAGGCACTGG TGTTGGTGGT GACCAGCACA
    TTTGGCAATG GGGATCCTCC GGAGAATGGA GAGAGCTTTG CAGCAGCGCT CATGGAAATG
    TCAGGCCCGT ACAACAGCTC CCCTAGGCCT GAGCAGCACA AGAGCTACAA AATCCGATTC
    AACAGTGTCT CCTGCTCAGA CCCACTGGTA TCCTCTTGGC GGCGCAAGAG GAAGGAGTCT
    AGCAACACAG ACAGTGCAGG AGCCCTGGGC ACCCTCAGGT TCTGTGTGTT TGGGCTGGGC
    TCCCGAGCAT ACCCCCACTT CTGTGCCTTT GCTCGAGCGG TGGACACAAG GCTGGAGGAG
    CTGGGCGGGG AGCGACTACT GCAGCTGGGC CAAGGTGATG AGCTCTGTGG CCAGGAGGAG
    GCTTTCCGAG GCTGGGCCCA GGCCGCCTTC CAGGCTGCCT GTGAAACCTT CTGTGTGGGA
    GAAGATGCCA AAGCTGCTGC CCGAGATATC TTCAGCCCCA AACGCAGCTG GAAGCGCCAG
    AGGTACCGGC TGAGTACCCA GGCTGAGAGC CTGCAATTAC TACCAGGGCT GACTCACGTG
    CACAGGCGGA AGATGTTCCA GGCTACAATC CTCTCTGTGG AAAACCTACA GAGCAGCAAA
    TCCACCCGAG CCACGATCCT GGTGCGTCTG GACACCGGAG GCCAGGAGGG ACTGCAGTAC
    CAGCCAGGGG ACCACATAGG TGTGTGCCCA CCCAACCGTC CTGGCCTAGT GGAGGCACTG
    CTGAGCCGAG TGGAGGACCC TCCGCCATCC ACAGAACCTG TGGCTGTGGA ACAACTGGAG
    AAAGGCAGCC CTGGTGGCCC TCCCCCCGGC TGGGTACGGG ACCCCCGGCT ACCCCCATGT
    ACGCTGCGGC AGGCTCTCAC CTACTTCCTG GACATCACTT CCCCGCCTAG TCCTCGCCTC
    CTTCGACTGC TCAGCACCCT GGCAGAAGAG TCCAGCGAAC AGCAGGAGCT AGAGGCTCTC
    AGCCAGGACC CCCGGCGCTA CGAAGAATGG AAGTGGTTCA GCTGCCCCAC ACTGCTAGAG
    GTGCTGGAGC AATTTCCTTC AGTGGCACTG CCTGCCCCGC TGATCCTCAC CCAGCTGCCC
    TTGCTCCAGC CCCGGTACTA CTCTGTCAGT TCAGCACCCA GCGCCCACCC AGGAGAGATC
    CACCTCACCA TAGCTGTGCT GGCTTACAGA ACCCAGGATG GGCTGGGCCC TCTGCACTAC
    GGTGTCTGCT CCACGTGGAT GAGCCAGCTC AAGGCGGGAG ATCCAGTGCC CTGCTTCATC
    AGGGGGGCTC CCTCCTTCCG GCTGCCACCT GATCCTAACT TGCCCTGCAT CCTGGTGGGC
    CCAGGGACTG GCATTGCACC CTTCCGGGGA TTCTGGCAAG ACAGACTACA CGACATTGAG
    ATCAAAGGGC TACAACCTGC CCCCATGACT TTGGTGTTTG GCTGCCGATG CTCCCAACTG
    GACCATCTCT ACCGGGACGA GGTACTGGAC GCCCAGCAGC GTGGGGTGTT TGGACAAGTC
    CTCACCGCCT TTTCCAGGGA TCCTGGCAGC CCCAAGACCT ACGTGCAAGA CCTCCTGAGG
    ACAGAGCTAG CCGCGGAGGT TCACCGTGTG CTGTGCCTTG AGCAAGGACA TATGTTTGTC
    TGCGGCGATG TCACTATGGC AACCAGCGTC CTGCAAACCG TGCAGAGAAT TCTGGCAACA
    GAGGGCGGCA TGGAGCTGGA TGAAGCCGGT GACGTCATCG GCGTGCTGCG GGATCAGCAA
    CGCTACCACG AGGACATTTT CGGACTCACA TTGCGCACCC AGGAGGTGAC AAGCCGCATA
    CGCACCCAGA GCTTTTCTTT GCAGGAGCGA CAGCTGAGGG GCGCAGTGCC CTGGTCCTTT
    GACCCGCCTG GCCCAGAAAT ACCTGGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006535702.1
    CDS204..3656
    Translation

    Target ORF information:

    RefSeq Version XM_006535639.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 3, endothelial cell (Nos3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006535639.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCAGCC CCCCGCTAAC CCGGCCCCCA GACGGACCCA GGTTTCCTCG AGTAAAGAAT 
    TGGGAAGTGG GCAGCATCAC CTACGACACC CTCAGTGCCC AGGCTCAGCA GGATGGGCCC
    TGTACCTCAA GACGCTGCTT GGGATCCCTG GTGTTTCCAA GGAAGTTACA GAGCCGGCCC
    ACCCAGGGCC CTTCACCCAC TGAGCAGCTA TTGGGTCAAG CCCGGGACTT CATCAATCAG
    TACTATAACT CCATCAAAAG GAGTGGCTCC CAGGCTCATG AGCAGCGGCT TCAGGAAGTG
    GAGGCTGAGG TGGCAGCCAC AGGCACCTAC CAGCTCCGGG AGAGCGAGCT GGTGTTTGGG
    GCCAAGCAGG CCTGGCGCAA TGCTCCCCGC TGTGTGGGCC GGATCCAGTG GGGAAAGCTG
    CAGGTATTTG ATGCTCGGGA CTGCAGGACT GCACAGGAAA TGTTCACCTA CATCTGTAAC
    CACATTAAAT ACGCAACAAA TAGAGGCAAT CTTCGTTCAG CCATCACAGT GTTCCCCCAG
    CGCTGCCCTG GCCGGGGAGA CTTCCGGATC TGGAACAGCC AGCTGATACG CTATGCGGGC
    TATAGGCAGC AGGATGGCTC CGTGCGAGGG GACCCCGCCA ACGTGGAGAT CACTGAGCTC
    TGTATCCAAC ATGGCTGGAC CCCAGGAAAT GGCCGCTTTG ATGTGCTGCC CCTGTTACTC
    CAGGCTCCTG ATGAGCCCCC AGAACTCTTC ACTCTGCCCC CAGAGATGGT CCTCGAGGTG
    CCTCTGGAGC ACCCCACGCT CGAGTGGTTT GCTGCCCTTG GCCTGCGCTG GTATGCCCTC
    CCAGCTGTGT CCAACATGCT GCTAGAAATC GGGGGCCTGG AGTTTCCTGC TGCCCCTTTC
    AGCGGCTGGT ACATGAGTTC AGAGATTGGC ATGAGGGACC TGTGTGACCC TCACCGCTAC
    AACATACTTG AGGATGTGGC TGTGTGCATG GATCTGGACA CCAGGACAAC CTCATCCCTG
    TGGAAAGACA AGGCAGCGGT GGAAATTAAT GTGGCCGTGT TGCACAGTTA CCAGCTGGCC
    AAAGTGACCA TAGTGGACCA CCACGCCGCC ACAGCCTCCT TCATGAAGCA CCTGGAAAAT
    GAGCAGAAGG CCAGAGGGGG CTGCCCTGCC GATTGGGCCT GGATTGTGCC CCCCATCTCA
    GGCAGCCTAA CTCCTGTCTT CCATCAAGAG ATGGTCAACT ATTTCCTGTC CCCTGCCTTC
    CGCTACCAGC CAGACCCCTG GAAGGGAAGT GCAGCAAAGG GGGCAGGCAT CACCAGGAAG
    AAGACCTTTA AGGAAGTAGC CAATGCAGTG AAGATCTCTG CCTCACTCAT GGGCACGGTG
    ATGGCGAAGC GTGTGAAGGC AACCATTCTG TATGGCTCTG AGACTGGCCG GGCCCAGAGC
    TACGCACAGC AGCTGGGAAG ACTCTTCCGG AAGGCGTTTG ATCCCCGGGT CCTGTGCATG
    GATGAGTATG ATGTGGTGTC CCTAGAGCAC GAGGCACTGG TGTTGGTGGT GACCAGCACA
    TTTGGCAATG GGGATCCTCC GGAGAATGGA GAGAGCTTTG CAGCAGCGCT CATGGAAATG
    TCAGGCCCGT ACAACAGCTC CCCTAGGCCT GAGCAGCACA AGAGCTACAA AATCCGATTC
    AACAGTGTCT CCTGCTCAGA CCCACTGGTA TCCTCTTGGC GGCGCAAGAG GAAGGAGTCT
    AGCAACACAG ACAGTGCAGG AGCCCTGGGC ACCCTCAGGT TCTGTGTGTT TGGGCTGGGC
    TCCCGAGCAT ACCCCCACTT CTGTGCCTTT GCTCGAGCGG TGGACACAAG GCTGGAGGAG
    CTGGGCGGGG AGCGACTACT GCAGCTGGGC CAAGGTGATG AGCTCTGTGG CCAGGAGGAG
    GCTTTCCGAG GCTGGGCCCA GGCCGCCTTC CAGGCTGCCT GTGAAACCTT CTGTGTGGGA
    GAAGATGCCA AAGCTGCTGC CCGAGATATC TTCAGCCCCA AACGCAGCTG GAAGCGCCAG
    AGGTACCGGC TGAGTACCCA GGCTGAGAGC CTGCAATTAC TACCAGGGCT GACTCACGTG
    CACAGGCGGA AGATGTTCCA GGCTACAATC CTCTCTGTGG AAAACCTACA GAGCAGCAAA
    TCCACCCGAG CCACGATCCT GGTGCGTCTG GACACCGGAG GCCAGGAGGG ACTGCAGTAC
    CAGCCAGGGG ACCACATAGG TGTGTGCCCA CCCAACCGTC CTGGCCTAGT GGAGGCACTG
    CTGAGCCGAG TGGAGGACCC TCCGCCATCC ACAGAACCTG TGGCTGTGGA ACAACTGGAG
    AAAGGCAGCC CTGGTGGCCC TCCCCCCGGC TGGGTACGGG ACCCCCGGCT ACCCCCATGT
    ACGCTGCGGC AGGCTCTCAC CTACTTCCTG GACATCACTT CCCCGCCTAG TCCTCGCCTC
    CTTCGACTGC TCAGCACCCT GGCAGAAGAG TCCAGCGAAC AGCAGGAGCT AGAGGCTCTC
    AGCCAGGACC CCCGGCGCTA CGAAGAATGG AAGTGGTTCA GCTGCCCCAC ACTGCTAGAG
    GTGCTGGAGC AATTTCCTTC AGTGGCACTG CCTGCCCCGC TGATCCTCAC CCAGCTGCCC
    TTGCTCCAGC CCCGGTACTA CTCTGTCAGT TCAGCACCCA GCGCCCACCC AGGAGAGATC
    CACCTCACCA TAGCTGTGCT GGCTTACAGA ACCCAGGATG GGCTGGGCCC TCTGCACTAC
    GGTGTCTGCT CCACGTGGAT GAGCCAGCTC AAGGCGGGAG ATCCAGTGCC CTGCTTCATC
    AGGGGGGCTC CCTCCTTCCG GCTGCCACCT GATCCTAACT TGCCCTGCAT CCTGGTGGGC
    CCAGGGACTG GCATTGCACC CTTCCGGGGA TTCTGGCAAG ACAGACTACA CGACATTGAG
    ATCAAAGGGC TACAACCTGC CCCCATGACT TTGGTGTTTG GCTGCCGATG CTCCCAACTG
    GACCATCTCT ACCGGGACGA GGTACTGGAC GCCCAGCAGC GTGGGGTGTT TGGACAAGTC
    CTCACCGCCT TTTCCAGGGA TCCTGGCAGC CCCAAGACCT ACGTGCAAGA CCTCCTGAGG
    ACAGAGCTAG CCGCGGAGGT TCACCGTGTG CTGTGCCTTG AGCAAGGACA TATGTTTGTC
    TGCGGCGATG TCACTATGGC AACCAGCGTC CTGCAAACCG TGCAGAGAAT TCTGGCAACA
    GAGGGCGGCA TGGAGCTGGA TGAAGCCGGT GACGTCATCG GCGTGCTGCG GGATCAGCAA
    CGCTACCACG AGGACATTTT CGGACTCACA TTGCGCACCC AGGAGGTGAC AAGCCGCATA
    CGCACCCAGA GCTTTTCTTT GCAGGAGCGA CAGCTGAGGG GCGCAGTGCC CTGGTCCTTT
    GACCCGCCTG GCCCAGAAAT ACCTGGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu34722
    Clone ID Related Accession (Same CDS sequence) XM_006535639.4 , XM_006535639.3
    Accession Version XM_006535639.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product nitric oxide synthase, endothelial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCAGCC CCCCGCTAAC CCGGCCCCCA GACGGACCCA GGTTTCCTCG AGTAAAGAAT 
    TGGGAAGTGG GCAGCATCAC CTACGACACC CTCAGTGCCC AGGCTCAGCA GGATGGGCCC
    TGTACCTCAA GACGCTGCTT GGGATCCCTG GTGTTTCCAA GGAAGTTACA GAGCCGGCCC
    ACCCAGGGCC CTTCACCCAC TGAGCAGCTA TTGGGTCAAG CCCGGGACTT CATCAATCAG
    TACTATAACT CCATCAAAAG GAGTGGCTCC CAGGCTCATG AGCAGCGGCT TCAGGAAGTG
    GAGGCTGAGG TGGCAGCCAC AGGCACCTAC CAGCTCCGGG AGAGCGAGCT GGTGTTTGGG
    GCCAAGCAGG CCTGGCGCAA TGCTCCCCGC TGTGTGGGCC GGATCCAGTG GGGAAAGCTG
    CAGGTATTTG ATGCTCGGGA CTGCAGGACT GCACAGGAAA TGTTCACCTA CATCTGTAAC
    CACATTAAAT ACGCAACAAA TAGAGGCAAT CTTCGTTCAG CCATCACAGT GTTCCCCCAG
    CGCTGCCCTG GCCGGGGAGA CTTCCGGATC TGGAACAGCC AGCTGATACG CTATGCGGGC
    TATAGGCAGC AGGATGGCTC CGTGCGAGGG GACCCCGCCA ACGTGGAGAT CACTGAGCTC
    TGTATCCAAC ATGGCTGGAC CCCAGGAAAT GGCCGCTTTG ATGTGCTGCC CCTGTTACTC
    CAGGCTCCTG ATGAGCCCCC AGAACTCTTC ACTCTGCCCC CAGAGATGGT CCTCGAGGTG
    CCTCTGGAGC ACCCCACGCT CGAGTGGTTT GCTGCCCTTG GCCTGCGCTG GTATGCCCTC
    CCAGCTGTGT CCAACATGCT GCTAGAAATC GGGGGCCTGG AGTTTCCTGC TGCCCCTTTC
    AGCGGCTGGT ACATGAGTTC AGAGATTGGC ATGAGGGACC TGTGTGACCC TCACCGCTAC
    AACATACTTG AGGATGTGGC TGTGTGCATG GATCTGGACA CCAGGACAAC CTCATCCCTG
    TGGAAAGACA AGGCAGCGGT GGAAATTAAT GTGGCCGTGT TGCACAGTTA CCAGCTGGCC
    AAAGTGACCA TAGTGGACCA CCACGCCGCC ACAGCCTCCT TCATGAAGCA CCTGGAAAAT
    GAGCAGAAGG CCAGAGGGGG CTGCCCTGCC GATTGGGCCT GGATTGTGCC CCCCATCTCA
    GGCAGCCTAA CTCCTGTCTT CCATCAAGAG ATGGTCAACT ATTTCCTGTC CCCTGCCTTC
    CGCTACCAGC CAGACCCCTG GAAGGGAAGT GCAGCAAAGG GGGCAGGCAT CACCAGGAAG
    AAGACCTTTA AGGAAGTAGC CAATGCAGTG AAGATCTCTG CCTCACTCAT GGGCACGGTG
    ATGGCGAAGC GTGTGAAGGC AACCATTCTG TATGGCTCTG AGACTGGCCG GGCCCAGAGC
    TACGCACAGC AGCTGGGAAG ACTCTTCCGG AAGGCGTTTG ATCCCCGGGT CCTGTGCATG
    GATGAGTATG ATGTGGTGTC CCTAGAGCAC GAGGCACTGG TGTTGGTGGT GACCAGCACA
    TTTGGCAATG GGGATCCTCC GGAGAATGGA GAGAGCTTTG CAGCAGCGCT CATGGAAATG
    TCAGGCCCGT ACAACAGCTC CCCTAGGCCT GAGCAGCACA AGAGCTACAA AATCCGATTC
    AACAGTGTCT CCTGCTCAGA CCCACTGGTA TCCTCTTGGC GGCGCAAGAG GAAGGAGTCT
    AGCAACACAG ACAGTGCAGG AGCCCTGGGC ACCCTCAGGT TCTGTGTGTT TGGGCTGGGC
    TCCCGAGCAT ACCCCCACTT CTGTGCCTTT GCTCGAGCGG TGGACACAAG GCTGGAGGAG
    CTGGGCGGGG AGCGACTACT GCAGCTGGGC CAAGGTGATG AGCTCTGTGG CCAGGAGGAG
    GCTTTCCGAG GCTGGGCCCA GGCCGCCTTC CAGGCTGCCT GTGAAACCTT CTGTGTGGGA
    GAAGATGCCA AAGCTGCTGC CCGAGATATC TTCAGCCCCA AACGCAGCTG GAAGCGCCAG
    AGGTACCGGC TGAGTACCCA GGCTGAGAGC CTGCAATTAC TACCAGGGCT GACTCACGTG
    CACAGGCGGA AGATGTTCCA GGCTACAATC CTCTCTGTGG AAAACCTACA GAGCAGCAAA
    TCCACCCGAG CCACGATCCT GGTGCGTCTG GACACCGGAG GCCAGGAGGG ACTGCAGTAC
    CAGCCAGGGG ACCACATAGG TGTGTGCCCA CCCAACCGTC CTGGCCTAGT GGAGGCACTG
    CTGAGCCGAG TGGAGGACCC TCCGCCATCC ACAGAACCTG TGGCTGTGGA ACAACTGGAG
    AAAGGCAGCC CTGGTGGCCC TCCCCCCGGC TGGGTACGGG ACCCCCGGCT ACCCCCATGT
    ACGCTGCGGC AGGCTCTCAC CTACTTCCTG GACATCACTT CCCCGCCTAG TCCTCGCCTC
    CTTCGACTGC TCAGCACCCT GGCAGAAGAG TCCAGCGAAC AGCAGGAGCT AGAGGCTCTC
    AGCCAGGACC CCCGGCGCTA CGAAGAATGG AAGTGGTTCA GCTGCCCCAC ACTGCTAGAG
    GTGCTGGAGC AATTTCCTTC AGTGGCACTG CCTGCCCCGC TGATCCTCAC CCAGCTGCCC
    TTGCTCCAGC CCCGGTACTA CTCTGTCAGT TCAGCACCCA GCGCCCACCC AGGAGAGATC
    CACCTCACCA TAGCTGTGCT GGCTTACAGA ACCCAGGATG GGCTGGGCCC TCTGCACTAC
    GGTGTCTGCT CCACGTGGAT GAGCCAGCTC AAGGCGGGAG ATCCAGTGCC CTGCTTCATC
    AGGGGGGCTC CCTCCTTCCG GCTGCCACCT GATCCTAACT TGCCCTGCAT CCTGGTGGGC
    CCAGGGACTG GCATTGCACC CTTCCGGGGA TTCTGGCAAG ACAGACTACA CGACATTGAG
    ATCAAAGGGC TACAACCTGC CCCCATGACT TTGGTGTTTG GCTGCCGATG CTCCCAACTG
    GACCATCTCT ACCGGGACGA GGTACTGGAC GCCCAGCAGC GTGGGGTGTT TGGACAAGTC
    CTCACCGCCT TTTCCAGGGA TCCTGGCAGC CCCAAGACCT ACGTGCAAGA CCTCCTGAGG
    ACAGAGCTAG CCGCGGAGGT TCACCGTGTG CTGTGCCTTG AGCAAGGACA TATGTTTGTC
    TGCGGCGATG TCACTATGGC AACCAGCGTC CTGCAAACCG TGCAGAGAAT TCTGGCAACA
    GAGGGCGGCA TGGAGCTGGA TGAAGCCGGT GACGTCATCG GCGTGCTGCG GGATCAGCAA
    CGCTACCACG AGGACATTTT CGGACTCACA TTGCGCACCC AGGAGGTGAC AAGCCGCATA
    CGCACCCAGA GCTTTTCTTT GCAGGAGCGA CAGCTGAGGG GCGCAGTGCC CTGGTCCTTT
    GACCCGCCTG GCCCAGAAAT ACCTGGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006535702.1
    CDS49..3501
    Translation

    Target ORF information:

    RefSeq Version XM_006535639.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus nitric oxide synthase 3, endothelial cell (Nos3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006535639.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCAGCC CCCCGCTAAC CCGGCCCCCA GACGGACCCA GGTTTCCTCG AGTAAAGAAT 
    TGGGAAGTGG GCAGCATCAC CTACGACACC CTCAGTGCCC AGGCTCAGCA GGATGGGCCC
    TGTACCTCAA GACGCTGCTT GGGATCCCTG GTGTTTCCAA GGAAGTTACA GAGCCGGCCC
    ACCCAGGGCC CTTCACCCAC TGAGCAGCTA TTGGGTCAAG CCCGGGACTT CATCAATCAG
    TACTATAACT CCATCAAAAG GAGTGGCTCC CAGGCTCATG AGCAGCGGCT TCAGGAAGTG
    GAGGCTGAGG TGGCAGCCAC AGGCACCTAC CAGCTCCGGG AGAGCGAGCT GGTGTTTGGG
    GCCAAGCAGG CCTGGCGCAA TGCTCCCCGC TGTGTGGGCC GGATCCAGTG GGGAAAGCTG
    CAGGTATTTG ATGCTCGGGA CTGCAGGACT GCACAGGAAA TGTTCACCTA CATCTGTAAC
    CACATTAAAT ACGCAACAAA TAGAGGCAAT CTTCGTTCAG CCATCACAGT GTTCCCCCAG
    CGCTGCCCTG GCCGGGGAGA CTTCCGGATC TGGAACAGCC AGCTGATACG CTATGCGGGC
    TATAGGCAGC AGGATGGCTC CGTGCGAGGG GACCCCGCCA ACGTGGAGAT CACTGAGCTC
    TGTATCCAAC ATGGCTGGAC CCCAGGAAAT GGCCGCTTTG ATGTGCTGCC CCTGTTACTC
    CAGGCTCCTG ATGAGCCCCC AGAACTCTTC ACTCTGCCCC CAGAGATGGT CCTCGAGGTG
    CCTCTGGAGC ACCCCACGCT CGAGTGGTTT GCTGCCCTTG GCCTGCGCTG GTATGCCCTC
    CCAGCTGTGT CCAACATGCT GCTAGAAATC GGGGGCCTGG AGTTTCCTGC TGCCCCTTTC
    AGCGGCTGGT ACATGAGTTC AGAGATTGGC ATGAGGGACC TGTGTGACCC TCACCGCTAC
    AACATACTTG AGGATGTGGC TGTGTGCATG GATCTGGACA CCAGGACAAC CTCATCCCTG
    TGGAAAGACA AGGCAGCGGT GGAAATTAAT GTGGCCGTGT TGCACAGTTA CCAGCTGGCC
    AAAGTGACCA TAGTGGACCA CCACGCCGCC ACAGCCTCCT TCATGAAGCA CCTGGAAAAT
    GAGCAGAAGG CCAGAGGGGG CTGCCCTGCC GATTGGGCCT GGATTGTGCC CCCCATCTCA
    GGCAGCCTAA CTCCTGTCTT CCATCAAGAG ATGGTCAACT ATTTCCTGTC CCCTGCCTTC
    CGCTACCAGC CAGACCCCTG GAAGGGAAGT GCAGCAAAGG GGGCAGGCAT CACCAGGAAG
    AAGACCTTTA AGGAAGTAGC CAATGCAGTG AAGATCTCTG CCTCACTCAT GGGCACGGTG
    ATGGCGAAGC GTGTGAAGGC AACCATTCTG TATGGCTCTG AGACTGGCCG GGCCCAGAGC
    TACGCACAGC AGCTGGGAAG ACTCTTCCGG AAGGCGTTTG ATCCCCGGGT CCTGTGCATG
    GATGAGTATG ATGTGGTGTC CCTAGAGCAC GAGGCACTGG TGTTGGTGGT GACCAGCACA
    TTTGGCAATG GGGATCCTCC GGAGAATGGA GAGAGCTTTG CAGCAGCGCT CATGGAAATG
    TCAGGCCCGT ACAACAGCTC CCCTAGGCCT GAGCAGCACA AGAGCTACAA AATCCGATTC
    AACAGTGTCT CCTGCTCAGA CCCACTGGTA TCCTCTTGGC GGCGCAAGAG GAAGGAGTCT
    AGCAACACAG ACAGTGCAGG AGCCCTGGGC ACCCTCAGGT TCTGTGTGTT TGGGCTGGGC
    TCCCGAGCAT ACCCCCACTT CTGTGCCTTT GCTCGAGCGG TGGACACAAG GCTGGAGGAG
    CTGGGCGGGG AGCGACTACT GCAGCTGGGC CAAGGTGATG AGCTCTGTGG CCAGGAGGAG
    GCTTTCCGAG GCTGGGCCCA GGCCGCCTTC CAGGCTGCCT GTGAAACCTT CTGTGTGGGA
    GAAGATGCCA AAGCTGCTGC CCGAGATATC TTCAGCCCCA AACGCAGCTG GAAGCGCCAG
    AGGTACCGGC TGAGTACCCA GGCTGAGAGC CTGCAATTAC TACCAGGGCT GACTCACGTG
    CACAGGCGGA AGATGTTCCA GGCTACAATC CTCTCTGTGG AAAACCTACA GAGCAGCAAA
    TCCACCCGAG CCACGATCCT GGTGCGTCTG GACACCGGAG GCCAGGAGGG ACTGCAGTAC
    CAGCCAGGGG ACCACATAGG TGTGTGCCCA CCCAACCGTC CTGGCCTAGT GGAGGCACTG
    CTGAGCCGAG TGGAGGACCC TCCGCCATCC ACAGAACCTG TGGCTGTGGA ACAACTGGAG
    AAAGGCAGCC CTGGTGGCCC TCCCCCCGGC TGGGTACGGG ACCCCCGGCT ACCCCCATGT
    ACGCTGCGGC AGGCTCTCAC CTACTTCCTG GACATCACTT CCCCGCCTAG TCCTCGCCTC
    CTTCGACTGC TCAGCACCCT GGCAGAAGAG TCCAGCGAAC AGCAGGAGCT AGAGGCTCTC
    AGCCAGGACC CCCGGCGCTA CGAAGAATGG AAGTGGTTCA GCTGCCCCAC ACTGCTAGAG
    GTGCTGGAGC AATTTCCTTC AGTGGCACTG CCTGCCCCGC TGATCCTCAC CCAGCTGCCC
    TTGCTCCAGC CCCGGTACTA CTCTGTCAGT TCAGCACCCA GCGCCCACCC AGGAGAGATC
    CACCTCACCA TAGCTGTGCT GGCTTACAGA ACCCAGGATG GGCTGGGCCC TCTGCACTAC
    GGTGTCTGCT CCACGTGGAT GAGCCAGCTC AAGGCGGGAG ATCCAGTGCC CTGCTTCATC
    AGGGGGGCTC CCTCCTTCCG GCTGCCACCT GATCCTAACT TGCCCTGCAT CCTGGTGGGC
    CCAGGGACTG GCATTGCACC CTTCCGGGGA TTCTGGCAAG ACAGACTACA CGACATTGAG
    ATCAAAGGGC TACAACCTGC CCCCATGACT TTGGTGTTTG GCTGCCGATG CTCCCAACTG
    GACCATCTCT ACCGGGACGA GGTACTGGAC GCCCAGCAGC GTGGGGTGTT TGGACAAGTC
    CTCACCGCCT TTTCCAGGGA TCCTGGCAGC CCCAAGACCT ACGTGCAAGA CCTCCTGAGG
    ACAGAGCTAG CCGCGGAGGT TCACCGTGTG CTGTGCCTTG AGCAAGGACA TATGTTTGTC
    TGCGGCGATG TCACTATGGC AACCAGCGTC CTGCAAACCG TGCAGAGAAT TCTGGCAACA
    GAGGGCGGCA TGGAGCTGGA TGAAGCCGGT GACGTCATCG GCGTGCTGCG GGATCAGCAA
    CGCTACCACG AGGACATTTT CGGACTCACA TTGCGCACCC AGGAGGTGAC AAGCCGCATA
    CGCACCCAGA GCTTTTCTTT GCAGGAGCGA CAGCTGAGGG GCGCAGTGCC CTGGTCCTTT
    GACCCGCCTG GCCCAGAAAT ACCTGGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.