Nme1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nme1
Entrez Gene ID	18102
Full Name	NME/NM23 nucleoside diphosphate kinase 1
Synonyms	AL024257,NDPK-A,NM23-M1,NM23A
General protein information	Preferred Names NME/NM23 nucleoside diphosphate kinase 1 Names nucleoside diphosphate kinase A NDK A NDP kinase A expressed in non-metastatic cells 1, protein metastasis inhibition factor NM23 non-metastatic cells 1, protein (NM23A) expressed in nucleoside-diphosphate kinase 1 nucleotide diphosphate kinase tumor metastatic process-associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_030245646.1	XP_030101506.1	nucleoside diphosphate kinase A isoform X1
NM_008704.2	NP_032730.1	nucleoside diphosphate kinase A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nme1	NP_612557.1
Pan troglodytes (chimpanzee)	NME1	NP_001191440.1
Canis lupus familiaris (dog)	NME1	NP_001019808.1
Arabidopsis thaliana (thale cress)	NDPK3	NP_192839.1
Homo sapiens (human)	NME1	NP_937818.1
Bos taurus (cattle)	NME1	NP_991387.2
Gallus gallus (chicken)	NME1	NP_001191690.1
Danio rerio (zebrafish)	nme2b.1	NP_571001.1
Arabidopsis thaliana (thale cress)	AT4G23895	NP_001190817.1
Oryza sativa (rice)	Os05g0595400	NP_001056515.1
Mus musculus (house mouse)	Nme1	NP_032730.1
Arabidopsis thaliana (thale cress)	AT4G23900	NP_567690.1

Gene Ontology
Bibliography

Related articles in PubMed

Metastasis suppressor NM23 limits oxidative stress in mammals by preventing activation of stress-activated protein kinases/JNKs through its nucleoside diphosphate kinase activity.
Peuchant E, Bats ML, Moranvillier I, Lepoivre M, Guitton J, Wendum D, Lacombe ML, Moreau-Gaudry F, Boissan M, Dabernat S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(4)1531-1546(2017 04)

PKC? is dispensible for oxLDL uptake and foam cell formation by human and murine macrophages.
Szilagyi K, Meijer AB, Neele AE, Verkuijlen P, Leitges M, Dabernat S, F?rster-Waldl E, Boztug K, Belot A, Kuijpers TW, Kraal G, de Winther MP, van den Berg TK
Cardiovascular research104(3)467-76(2014 Dec)

An EMMPRIN-?-catenin-Nm23 complex drives ATP production and actomyosin contractility at endothelial junctions.
Moreno V, Gonzalo P, G?mez-Escudero J, Poll?n ?, Ac?n-P?rez R, Breckenridge M, Y??ez-M? M, Barreiro O, Orsenigo F, Kadomatsu K, Chen CS, Enr?quez JA, Dejana E, S?nchez-Madrid F, Arroyo AG
Journal of cell science127(Pt 17)3768-81(2014 Sep)

Membrane trafficking. Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling.
Boissan M, Montagnac G, Shen Q, Griparic L, Guitton J, Romao M, Sauvonnet N, Lagache T, Lascu I, Raposo G, Desbourdes C, Schlattner U, Lacombe ML, Polo S, van der Bliek AM, Roux A, Chavrier P
Science (New York, N.Y.)344(6191)1510-5(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deletion of NME1 reduced total NDPK activity and exacerbated activation of the stress-related MAPK, JNK, in the liver in response to paraquat. NDPK activity protects cells from acute oxidative stress by inhibiting activation of JNK in mammal models.
Title: Metastasis suppressor NM23 limits oxidative stress in mammals by preventing activation of stress-activated protein kinases/JNKs through its nucleoside diphosphate kinase activity.

EMMPRIN ensures proper actomyosin-driven maturation of competent endothelial junctions by forming a molecular complex with gamma-catenin (also known as junction plakoglobin) and Nm23 (also known as NME1), a nucleoside diphosphate kinase
Title: An EMMPRIN-?-catenin-Nm23 complex drives ATP production and actomyosin contractility at endothelial junctions.

NM23 deficiency promotes metastasis in a UV radiation-induced mouse model of human melanoma.
Title: NM23 deficiency promotes metastasis in a UV radiation-induced mouse model of human melanoma.

data suggested that NME1 acts as a switcher or reprogramming factor which involves in oligodentrocyte versus neuron cell fate specification in vitro
Title: Nucleoside diphosphate kinase Nm23-M1 involves in oligodendroglial versus neuronal cell fate decision in vitro.

data show a critical role for NM23 isoforms in limiting mutagenesis and suppressing UVR-induced melanomagenesis
Title: Metastasis suppressor NM23-H1 promotes repair of UV-induced DNA damage and suppresses UV-induced melanomagenesis.

The following Nme1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nme1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14681	NM_008704.2 Latest version!	Mus musculus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu85651	XM_030245646.1 Latest version!	Mus musculus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nme1 ( NM_008704.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032730.1 Mus musculus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), mRNA.

Price & Availability↑

CloneID	OMu14681
Clone ID Related Accession (Same CDS sequence)	NM_008704.2
Accession Version	NM_008704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Mus musculus(house mouse)
Product	nucleoside diphosphate kinase A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY037001.1 and AK034899.1. On or before Dec 7, 2005 this sequence version replaced XM_914818.1, NM_008704.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U85511.1, SRR1660821.284357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACA GTGAGCGCAC CTTCATTGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTG 
GTGGGCGAGA TCATCAAGCG GTTCGAGCAG AAGGGGTTCC GCCTTGTTGG TCTGAAGTTT 
CTGCAGGCTT CAGAGGACCT TCTCAAGGAG CACTACACTG ACCTGAAGGA CCGCCCCTTC 
TTTACTGGCC TGGTGAAATA CATGCACTCA GGACCAGTGG TTGCTATGGT CTGGGAGGGT 
CTGAATGTGG TGAAGACAGG CCGCGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTCTGCATC CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTAA AGAGCGCAGA GAAGGAGATC AGCTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GAGTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032730.1
CDS	306..764
Translation	MANSERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFLQASEDLLKEHYTDLKDRPFFTGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFQPEELVEYKSCAQNWIYE

Target ORF information:

RefSeq Version	NM_008704.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008704.2

ORF Insert Sequence:

ATGGCCAACA GTGAGCGCAC CTTCATTGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTG 
GTGGGCGAGA TCATCAAGCG GTTCGAGCAG AAGGGGTTCC GCCTTGTTGG TCTGAAGTTT 
CTGCAGGCTT CAGAGGACCT TCTCAAGGAG CACTACACTG ACCTGAAGGA CCGCCCCTTC 
TTTACTGGCC TGGTGAAATA CATGCACTCA GGACCAGTGG TTGCTATGGT CTGGGAGGGT 
CTGAATGTGG TGAAGACAGG CCGCGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTCTGCATC CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTAA AGAGCGCAGA GAAGGAGATC AGCTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GAGTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nme1 ( XM_030245646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101506.1 Mus musculus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu85651
Clone ID Related Accession (Same CDS sequence)	XM_030245646.1
Accession Version	XM_030245646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nucleoside diphosphate kinase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTATT CTCTTTCCAG TCCGCTGTTG GGCACAGAGA CCATGGCCAA CAGTGAGCGC 
ACCTTCATTG CCATCAAGCC TGATGGGGTC CAGCGGGGGC TGGTGGGCGA GATCATCAAG 
CGGTTCGAGC AGAAGGGGTT CCGCCTTGTT GGTCTGAAGT TTCTGCAGGC TTCAGAGGAC 
CTTCTCAAGG AGCACTACAC TGACCTGAAG GACCGCCCCT TCTTTACTGG CCTGGTGAAA 
TACATGCACT CAGGACCAGT GGTTGCTATG GTCTGGGAGG GTCTGAATGT GGTGAAGACA 
GGCCGCGTGA TGCTTGGAGA GACCAACCCC GCAGACTCTA AGCCTGGGAC CATACGAGGA 
GACTTCTGCA TCCAAGTTGG CAGGAACATC ATTCATGGCA GCGATTCTGT AAAGAGCGCA 
GAGAAGGAGA TCAGCTTGTG GTTTCAGCCT GAGGAGCTGG TGGAGTACAA GAGCTGTGCG 
CAGAACTGGA TCTATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101506.1
CDS	56..556
Translation	MPYSLSSPLLGTETMANSERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFLQASEDLLKEHYTDLKDRPFFTGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFQPEELVEYKSCAQNWIYE

Target ORF information:

RefSeq Version	XM_030245646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245646.1

ORF Insert Sequence:

ATGCCTTATT CTCTTTCCAG TCCGCTGTTG GGCACAGAGA CCATGGCCAA CAGTGAGCGC 
ACCTTCATTG CCATCAAGCC TGATGGGGTC CAGCGGGGGC TGGTGGGCGA GATCATCAAG 
CGGTTCGAGC AGAAGGGGTT CCGCCTTGTT GGTCTGAAGT TTCTGCAGGC TTCAGAGGAC 
CTTCTCAAGG AGCACTACAC TGACCTGAAG GACCGCCCCT TCTTTACTGG CCTGGTGAAA 
TACATGCACT CAGGACCAGT GGTTGCTATG GTCTGGGAGG GTCTGAATGT GGTGAAGACA 
GGCCGCGTGA TGCTTGGAGA GACCAACCCC GCAGACTCTA AGCCTGGGAC CATACGAGGA 
GACTTCTGCA TCCAAGTTGG CAGGAACATC ATTCATGGCA GCGATTCTGT AAAGAGCGCA 
GAGAAGGAGA TCAGCTTGTG GTTTCAGCCT GAGGAGCTGG TGGAGTACAA GAGCTGTGCG 
CAGAACTGGA TCTATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metastasis suppressor NM23 limits oxidative stress in mammals by preventing activation of stress-activated protein kinases/JNKs through its nucleoside diphosphate kinase activity.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(4)1531-1546(2017 04)
Peuchant E,Bats ML,Moranvillier I,Lepoivre M,Guitton J,Wendum D,Lacombe ML,Moreau-Gaudry F,Boissan M,Dabernat S

PKC? is dispensible for oxLDL uptake and foam cell formation by human and murine macrophages.
Cardiovascular research104(3)467-76(2014 Dec)
Szilagyi K,Meijer AB,Neele AE,Verkuijlen P,Leitges M,Dabernat S,F?rster-Waldl E,Boztug K,Belot A,Kuijpers TW,Kraal G,de Winther MP,van den Berg TK

An EMMPRIN-?-catenin-Nm23 complex drives ATP production and actomyosin contractility at endothelial junctions.
Journal of cell science127(Pt 17)3768-81(2014 Sep)
Moreno V,Gonzalo P,G?mez-Escudero J,Poll?n ?,Ac?n-P?rez R,Breckenridge M,Y??ez-M? M,Barreiro O,Orsenigo F,Kadomatsu K,Chen CS,Enr?quez JA,Dejana E,S?nchez-Madrid F,Arroyo AG

Membrane trafficking. Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling.
Science (New York, N.Y.)344(6191)1510-5(2014 Jun)
Boissan M,Montagnac G,Shen Q,Griparic L,Guitton J,Romao M,Sauvonnet N,Lagache T,Lascu I,Raposo G,Desbourdes C,Schlattner U,Lacombe ML,Polo S,van der Bliek AM,Roux A,Chavrier P

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Nme1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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