Ngf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ngf
Entrez Gene ID	18049
Full Name	nerve growth factor
Synonyms	Ngfb,beta-NGF
General protein information	Preferred Names nerve growth factor Names beta-nerve growth factor nerve growth factor, beta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 F2.2\|3 45.25 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001112698.2	NP_001106168.1	beta-nerve growth factor isoform B precursor
NM_013609.3	NP_038637.1	beta-nerve growth factor isoform A
XM_006501108.3	XP_006501171.1	beta-nerve growth factor isoform X1
XM_006501108.4	XP_006501171.1	beta-nerve growth factor isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ngf	NP_001123396.1
	LOC711681	XP_001100522.2
Mus musculus (house mouse)	Ngf	NP_038637.1
Pan troglodytes (chimpanzee)	NGF	NP_001012439.1
Homo sapiens (human)	NGF	NP_002497.2
Rattus norvegicus (Norway rat)	Ngf	NP_001263984.1
Canis lupus familiaris (dog)	NGF	NP_001181879.1
Bos taurus (cattle)	NGF	NP_001092832.1
Gallus gallus (chicken)	NGF	XP_004935065.1

Gene Ontology
Bibliography

Related articles in PubMed

Chromosomal locations of the human and mouse genes for precursors of epidermal growth factor and the beta subunit of nerve growth factor.
Zabel BU, Eddy RL, Lalley PA, Scott J, Bell GI, Shows TB
Proceedings of the National Academy of Sciences of the United States of America82(2)469-73(1985 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mechanical stress-induced upregulation of NGF in colon SMC underlies the visceral hypersensitivity in bowel obstruction through increased gene expression and activity of tetrodotoxin-resistant Na channels in sensory neurons.
Title: Pathogenesis of abdominal pain in bowel obstruction: role of mechanical stress-induced upregulation of nerve growth factor in gut smooth muscle cells.

These findings reveal a non-neuronal role for neurotrophins and identify a new regulatory pathway in insulin secretion that can be targeted to ameliorate beta cell dysfunction.
Title: Neurotrophin Signaling Is Required for Glucose-Induced Insulin Secretion.

nerve growth factor (NGF) signaling through neurotrophic tyrosine kinase receptor type 1 (TrkA) directs innervation of the developing mouse femur to promote vascularization and osteoprogenitor lineage progression.
Title: NGF-TrkA Signaling by Sensory Nerves Coordinates the Vascularization and Ossification of Developing Endochondral Bone.

results suggest that perivascular nerves innervate neovessels as neovasculatures mature and that NGF accelerates the innervation of perivascular nerves in neovessels.
Title: Nerve growth factor facilitates perivascular innervation in neovasculatures of mice.

TNF-alpha upregulated Nerve Growth Factor [NGF] expression in synovial fibroblasts and macrophages and IL-1beta upregulated NGF expression in synovial fibroblasts. IL-1beta and TNF-alpha may regulate NGF signaling in Osteoarthritic joints and be suitable therapeutic targets for treating Osteoarthritis pain
Title: Nerve Growth Factor Regulation by TNF-? and IL-1? in Synovial Macrophages and Fibroblasts in Osteoarthritic Mice.

The following Ngf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ngf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19799	NM_013609.3 Latest version!	Mus musculus nerve growth factor (Ngf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu19834	NM_001112698.2 Latest version!	Mus musculus nerve growth factor (Ngf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu19799	XM_006501108.4 Latest version!	Mus musculus nerve growth factor (Ngf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu19799	XM_006501108.3	Mus musculus nerve growth factor (Ngf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ngf ( NM_013609.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038637.1 Mus musculus nerve growth factor (Ngf), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19799
Clone ID Related Accession (Same CDS sequence)	NM_013609.3 , XM_006501108.3 , XM_006501108.4
Accession Version	NM_013609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	beta-nerve growth factor isoform A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BF786523.1, AK144588.1 and K01759.1. On Nov 20, 2013 this sequence version replaced NM_013609.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144588.1, M35075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTGCC TCAAGCCAGT GAAATTAGGC TCCCTGGAGG TGGGACACGG GCAGCATGGT 
GGAGTTTTGG CCTGTGGTCG TGCAGTCCAG GGGGCTGGAT GGCATGCTGG ACCCAAGCTC 
ACCTCAGTGT CTGGGCCCAA TAAAGGTTTT GCCAAGGACG CAGCTTTCTA TACTGGCCGC 
AGTGAGGTGC ATAGCGTAAT GTCCATGTTG TTCTACACTC TGATCACTGC GTTTTTGATC 
GGCGTACAGG CAGAACCGTA CACAGATAGC AATGTCCCAG AAGGAGACTC TGTCCCTGAA 
GCCCACTGGA CTAAACTTCA GCATTCCCTT GACACAGCCC TCCGCAGAGC CCGCAGTGCC 
CCTACTGCAC CAATAGCTGC CCGAGTGACA GGGCAGACCC GCAACATCAC TGTAGACCCC 
AGACTGTTTA AGAAACGGAG ACTCCACTCA CCCCGTGTGC TGTTCAGCAC CCAGCCTCCA 
CCCACCTCTT CAGACACTCT GGATCTAGAC TTCCAGGCCC ATGGTACAAT CCCTTTCAAC 
AGGACTCACC GGAGCAAGCG CTCATCCACC CACCCAGTCT TCCACATGGG GGAGTTCTCA 
GTGTGTGACA GTGTCAGTGT GTGGGTTGGA GATAAGACCA CAGCCACAGA CATCAAGGGC 
AAGGAGGTGA CAGTGCTGGC CGAGGTGAAC ATTAACAACA GTGTATTCAG ACAGTACTTT 
TTTGAGACCA AGTGCCGAGC CTCCAATCCT GTTGAGAGTG GGTGCCGGGG CATCGACTCC 
AAACACTGGA ACTCATACTG CACCACGACT CACACCTTCG TCAAGGCGTT GACAACAGAT 
GAGAAGCAGG CTGCCTGGAG GTTCATCCGG ATAGACACAG CCTGTGTGTG TGTGCTCAGC 
AGGAAGGCTA CAAGAAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038637.1
CDS	108..1031
Translation	MLCLKPVKLGSLEVGHGQHGGVLACGRAVQGAGWHAGPKLTSVSGPNKGFAKDAAFYTGRSEVHSVMSMLFYTLITAFLIGVQAEPYTDSNVPEGDSVPEAHWTKLQHSLDTALRRARSAPTAPIAARVTGQTRNITVDPRLFKKRRLHSPRVLFSTQPPPTSSDTLDLDFQAHGTIPFNRTHRSKRSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG

Target ORF information:

RefSeq Version	NM_013609.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nerve growth factor (Ngf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013609.3

ORF Insert Sequence:

ATGCTGTGCC TCAAGCCAGT GAAATTAGGC TCCCTGGAGG TGGGACACGG GCAGCATGGT 
GGAGTTTTGG CCTGTGGTCG TGCAGTCCAG GGGGCTGGAT GGCATGCTGG ACCCAAGCTC 
ACCTCAGTGT CTGGGCCCAA TAAAGGTTTT GCCAAGGACG CAGCTTTCTA TACTGGCCGC 
AGTGAGGTGC ATAGCGTAAT GTCCATGTTG TTCTACACTC TGATCACTGC GTTTTTGATC 
GGCGTACAGG CAGAACCGTA CACAGATAGC AATGTCCCAG AAGGAGACTC TGTCCCTGAA 
GCCCACTGGA CTAAACTTCA GCATTCCCTT GACACAGCCC TCCGCAGAGC CCGCAGTGCC 
CCTACTGCAC CAATAGCTGC CCGAGTGACA GGGCAGACCC GCAACATCAC TGTAGACCCC 
AGACTGTTTA AGAAACGGAG ACTCCACTCA CCCCGTGTGC TGTTCAGCAC CCAGCCTCCA 
CCCACCTCTT CAGACACTCT GGATCTAGAC TTCCAGGCCC ATGGTACAAT CCCTTTCAAC 
AGGACTCACC GGAGCAAGCG CTCATCCACC CACCCAGTCT TCCACATGGG GGAGTTCTCA 
GTGTGTGACA GTGTCAGTGT GTGGGTTGGA GATAAGACCA CAGCCACAGA CATCAAGGGC 
AAGGAGGTGA CAGTGCTGGC CGAGGTGAAC ATTAACAACA GTGTATTCAG ACAGTACTTT 
TTTGAGACCA AGTGCCGAGC CTCCAATCCT GTTGAGAGTG GGTGCCGGGG CATCGACTCC 
AAACACTGGA ACTCATACTG CACCACGACT CACACCTTCG TCAAGGCGTT GACAACAGAT 
GAGAAGCAGG CTGCCTGGAG GTTCATCCGG ATAGACACAG CCTGTGTGTG TGTGCTCAGC 
AGGAAGGCTA CAAGAAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ngf ( NM_001112698.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106168.1 Mus musculus nerve growth factor (Ngf), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19834
Clone ID Related Accession (Same CDS sequence)	NM_001112698.2
Accession Version	NM_001112698.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	beta-nerve growth factor isoform B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108822.9. On Nov 20, 2013 this sequence version replaced NM_001112698.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region compared to variant 1. The resulting isoform (B) has a shorter N-terminus compared to isoform A. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF786523.1, BY334442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCATGT TGTTCTACAC TCTGATCACT GCGTTTTTGA TCGGCGTACA GGCAGAACCG 
TACACAGATA GCAATGTCCC AGAAGGAGAC TCTGTCCCTG AAGCCCACTG GACTAAACTT 
CAGCATTCCC TTGACACAGC CCTCCGCAGA GCCCGCAGTG CCCCTACTGC ACCAATAGCT 
GCCCGAGTGA CAGGGCAGAC CCGCAACATC ACTGTAGACC CCAGACTGTT TAAGAAACGG 
AGACTCCACT CACCCCGTGT GCTGTTCAGC ACCCAGCCTC CACCCACCTC TTCAGACACT 
CTGGATCTAG ACTTCCAGGC CCATGGTACA ATCCCTTTCA ACAGGACTCA CCGGAGCAAG 
CGCTCATCCA CCCACCCAGT CTTCCACATG GGGGAGTTCT CAGTGTGTGA CAGTGTCAGT 
GTGTGGGTTG GAGATAAGAC CACAGCCACA GACATCAAGG GCAAGGAGGT GACAGTGCTG 
GCCGAGGTGA ACATTAACAA CAGTGTATTC AGACAGTACT TTTTTGAGAC CAAGTGCCGA 
GCCTCCAATC CTGTTGAGAG TGGGTGCCGG GGCATCGACT CCAAACACTG GAACTCATAC 
TGCACCACGA CTCACACCTT CGTCAAGGCG TTGACAACAG ATGAGAAGCA GGCTGCCTGG 
AGGTTCATCC GGATAGACAC AGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TACAAGAAGA 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106168.1
CDS	179..904
Translation	MSMLFYTLITAFLIGVQAEPYTDSNVPEGDSVPEAHWTKLQHSLDTALRRARSAPTAPIAARVTGQTRNITVDPRLFKKRRLHSPRVLFSTQPPPTSSDTLDLDFQAHGTIPFNRTHRSKRSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG

Target ORF information:

RefSeq Version	NM_001112698.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nerve growth factor (Ngf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112698.2

ORF Insert Sequence:

ATGTCCATGT TGTTCTACAC TCTGATCACT GCGTTTTTGA TCGGCGTACA GGCAGAACCG 
TACACAGATA GCAATGTCCC AGAAGGAGAC TCTGTCCCTG AAGCCCACTG GACTAAACTT 
CAGCATTCCC TTGACACAGC CCTCCGCAGA GCCCGCAGTG CCCCTACTGC ACCAATAGCT 
GCCCGAGTGA CAGGGCAGAC CCGCAACATC ACTGTAGACC CCAGACTGTT TAAGAAACGG 
AGACTCCACT CACCCCGTGT GCTGTTCAGC ACCCAGCCTC CACCCACCTC TTCAGACACT 
CTGGATCTAG ACTTCCAGGC CCATGGTACA ATCCCTTTCA ACAGGACTCA CCGGAGCAAG 
CGCTCATCCA CCCACCCAGT CTTCCACATG GGGGAGTTCT CAGTGTGTGA CAGTGTCAGT 
GTGTGGGTTG GAGATAAGAC CACAGCCACA GACATCAAGG GCAAGGAGGT GACAGTGCTG 
GCCGAGGTGA ACATTAACAA CAGTGTATTC AGACAGTACT TTTTTGAGAC CAAGTGCCGA 
GCCTCCAATC CTGTTGAGAG TGGGTGCCGG GGCATCGACT CCAAACACTG GAACTCATAC 
TGCACCACGA CTCACACCTT CGTCAAGGCG TTGACAACAG ATGAGAAGCA GGCTGCCTGG 
AGGTTCATCC GGATAGACAC AGCCTGTGTG TGTGTGCTCA GCAGGAAGGC TACAAGAAGA 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ngf ( XM_006501108.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501171.1 Mus musculus nerve growth factor (Ngf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19799
Clone ID Related Accession (Same CDS sequence)	NM_013609.3 , XM_006501108.3 , XM_006501108.4
Accession Version	XM_006501108.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	beta-nerve growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCC TCAAGCCAGT GAAATTAGGC TCCCTGGAGG TGGGACACGG GCAGCATGGT 
GGAGTTTTGG CCTGTGGTCG TGCAGTCCAG GGGGCTGGAT GGCATGCTGG ACCCAAGCTC 
ACCTCAGTGT CTGGGCCCAA TAAAGGTTTT GCCAAGGACG CAGCTTTCTA TACTGGCCGC 
AGTGAGGTGC ATAGCGTAAT GTCCATGTTG TTCTACACTC TGATCACTGC GTTTTTGATC 
GGCGTACAGG CAGAACCGTA CACAGATAGC AATGTCCCAG AAGGAGACTC TGTCCCTGAA 
GCCCACTGGA CTAAACTTCA GCATTCCCTT GACACAGCCC TCCGCAGAGC CCGCAGTGCC 
CCTACTGCAC CAATAGCTGC CCGAGTGACA GGGCAGACCC GCAACATCAC TGTAGACCCC 
AGACTGTTTA AGAAACGGAG ACTCCACTCA CCCCGTGTGC TGTTCAGCAC CCAGCCTCCA 
CCCACCTCTT CAGACACTCT GGATCTAGAC TTCCAGGCCC ATGGTACAAT CCCTTTCAAC 
AGGACTCACC GGAGCAAGCG CTCATCCACC CACCCAGTCT TCCACATGGG GGAGTTCTCA 
GTGTGTGACA GTGTCAGTGT GTGGGTTGGA GATAAGACCA CAGCCACAGA CATCAAGGGC 
AAGGAGGTGA CAGTGCTGGC CGAGGTGAAC ATTAACAACA GTGTATTCAG ACAGTACTTT 
TTTGAGACCA AGTGCCGAGC CTCCAATCCT GTTGAGAGTG GGTGCCGGGG CATCGACTCC 
AAACACTGGA ACTCATACTG CACCACGACT CACACCTTCG TCAAGGCGTT GACAACAGAT 
GAGAAGCAGG CTGCCTGGAG GTTCATCCGG ATAGACACAG CCTGTGTGTG TGTGCTCAGC 
AGGAAGGCTA CAAGAAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501171.1
CDS	178..1101
Translation	MLCLKPVKLGSLEVGHGQHGGVLACGRAVQGAGWHAGPKLTSVSGPNKGFAKDAAFYTGRSEVHSVMSMLFYTLITAFLIGVQAEPYTDSNVPEGDSVPEAHWTKLQHSLDTALRRARSAPTAPIAARVTGQTRNITVDPRLFKKRRLHSPRVLFSTQPPPTSSDTLDLDFQAHGTIPFNRTHRSKRSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG

Target ORF information:

RefSeq Version	XM_006501108.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nerve growth factor (Ngf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501108.3

ORF Insert Sequence:

ATGCTGTGCC TCAAGCCAGT GAAATTAGGC TCCCTGGAGG TGGGACACGG GCAGCATGGT 
GGAGTTTTGG CCTGTGGTCG TGCAGTCCAG GGGGCTGGAT GGCATGCTGG ACCCAAGCTC 
ACCTCAGTGT CTGGGCCCAA TAAAGGTTTT GCCAAGGACG CAGCTTTCTA TACTGGCCGC 
AGTGAGGTGC ATAGCGTAAT GTCCATGTTG TTCTACACTC TGATCACTGC GTTTTTGATC 
GGCGTACAGG CAGAACCGTA CACAGATAGC AATGTCCCAG AAGGAGACTC TGTCCCTGAA 
GCCCACTGGA CTAAACTTCA GCATTCCCTT GACACAGCCC TCCGCAGAGC CCGCAGTGCC 
CCTACTGCAC CAATAGCTGC CCGAGTGACA GGGCAGACCC GCAACATCAC TGTAGACCCC 
AGACTGTTTA AGAAACGGAG ACTCCACTCA CCCCGTGTGC TGTTCAGCAC CCAGCCTCCA 
CCCACCTCTT CAGACACTCT GGATCTAGAC TTCCAGGCCC ATGGTACAAT CCCTTTCAAC 
AGGACTCACC GGAGCAAGCG CTCATCCACC CACCCAGTCT TCCACATGGG GGAGTTCTCA 
GTGTGTGACA GTGTCAGTGT GTGGGTTGGA GATAAGACCA CAGCCACAGA CATCAAGGGC 
AAGGAGGTGA CAGTGCTGGC CGAGGTGAAC ATTAACAACA GTGTATTCAG ACAGTACTTT 
TTTGAGACCA AGTGCCGAGC CTCCAATCCT GTTGAGAGTG GGTGCCGGGG CATCGACTCC 
AAACACTGGA ACTCATACTG CACCACGACT CACACCTTCG TCAAGGCGTT GACAACAGAT 
GAGAAGCAGG CTGCCTGGAG GTTCATCCGG ATAGACACAG CCTGTGTGTG TGTGCTCAGC 
AGGAAGGCTA CAAGAAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ngf ( XM_006501108.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501171.1 Mus musculus nerve growth factor (Ngf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19799
Clone ID Related Accession (Same CDS sequence)	NM_013609.3 , XM_006501108.3 , XM_006501108.4
Accession Version	XM_006501108.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta-nerve growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501108.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCC TCAAGCCAGT GAAATTAGGC TCCCTGGAGG TGGGACACGG GCAGCATGGT 
GGAGTTTTGG CCTGTGGTCG TGCAGTCCAG GGGGCTGGAT GGCATGCTGG ACCCAAGCTC 
ACCTCAGTGT CTGGGCCCAA TAAAGGTTTT GCCAAGGACG CAGCTTTCTA TACTGGCCGC 
AGTGAGGTGC ATAGCGTAAT GTCCATGTTG TTCTACACTC TGATCACTGC GTTTTTGATC 
GGCGTACAGG CAGAACCGTA CACAGATAGC AATGTCCCAG AAGGAGACTC TGTCCCTGAA 
GCCCACTGGA CTAAACTTCA GCATTCCCTT GACACAGCCC TCCGCAGAGC CCGCAGTGCC 
CCTACTGCAC CAATAGCTGC CCGAGTGACA GGGCAGACCC GCAACATCAC TGTAGACCCC 
AGACTGTTTA AGAAACGGAG ACTCCACTCA CCCCGTGTGC TGTTCAGCAC CCAGCCTCCA 
CCCACCTCTT CAGACACTCT GGATCTAGAC TTCCAGGCCC ATGGTACAAT CCCTTTCAAC 
AGGACTCACC GGAGCAAGCG CTCATCCACC CACCCAGTCT TCCACATGGG GGAGTTCTCA 
GTGTGTGACA GTGTCAGTGT GTGGGTTGGA GATAAGACCA CAGCCACAGA CATCAAGGGC 
AAGGAGGTGA CAGTGCTGGC CGAGGTGAAC ATTAACAACA GTGTATTCAG ACAGTACTTT 
TTTGAGACCA AGTGCCGAGC CTCCAATCCT GTTGAGAGTG GGTGCCGGGG CATCGACTCC 
AAACACTGGA ACTCATACTG CACCACGACT CACACCTTCG TCAAGGCGTT GACAACAGAT 
GAGAAGCAGG CTGCCTGGAG GTTCATCCGG ATAGACACAG CCTGTGTGTG TGTGCTCAGC 
AGGAAGGCTA CAAGAAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501171.1
CDS	826..1749
Translation	MLCLKPVKLGSLEVGHGQHGGVLACGRAVQGAGWHAGPKLTSVSGPNKGFAKDAAFYTGRSEVHSVMSMLFYTLITAFLIGVQAEPYTDSNVPEGDSVPEAHWTKLQHSLDTALRRARSAPTAPIAARVTGQTRNITVDPRLFKKRRLHSPRVLFSTQPPPTSSDTLDLDFQAHGTIPFNRTHRSKRSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG

Target ORF information:

RefSeq Version	XM_006501108.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nerve growth factor (Ngf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501108.4

ORF Insert Sequence:

ATGCTGTGCC TCAAGCCAGT GAAATTAGGC TCCCTGGAGG TGGGACACGG GCAGCATGGT 
GGAGTTTTGG CCTGTGGTCG TGCAGTCCAG GGGGCTGGAT GGCATGCTGG ACCCAAGCTC 
ACCTCAGTGT CTGGGCCCAA TAAAGGTTTT GCCAAGGACG CAGCTTTCTA TACTGGCCGC 
AGTGAGGTGC ATAGCGTAAT GTCCATGTTG TTCTACACTC TGATCACTGC GTTTTTGATC 
GGCGTACAGG CAGAACCGTA CACAGATAGC AATGTCCCAG AAGGAGACTC TGTCCCTGAA 
GCCCACTGGA CTAAACTTCA GCATTCCCTT GACACAGCCC TCCGCAGAGC CCGCAGTGCC 
CCTACTGCAC CAATAGCTGC CCGAGTGACA GGGCAGACCC GCAACATCAC TGTAGACCCC 
AGACTGTTTA AGAAACGGAG ACTCCACTCA CCCCGTGTGC TGTTCAGCAC CCAGCCTCCA 
CCCACCTCTT CAGACACTCT GGATCTAGAC TTCCAGGCCC ATGGTACAAT CCCTTTCAAC 
AGGACTCACC GGAGCAAGCG CTCATCCACC CACCCAGTCT TCCACATGGG GGAGTTCTCA 
GTGTGTGACA GTGTCAGTGT GTGGGTTGGA GATAAGACCA CAGCCACAGA CATCAAGGGC 
AAGGAGGTGA CAGTGCTGGC CGAGGTGAAC ATTAACAACA GTGTATTCAG ACAGTACTTT 
TTTGAGACCA AGTGCCGAGC CTCCAATCCT GTTGAGAGTG GGTGCCGGGG CATCGACTCC 
AAACACTGGA ACTCATACTG CACCACGACT CACACCTTCG TCAAGGCGTT GACAACAGAT 
GAGAAGCAGG CTGCCTGGAG GTTCATCCGG ATAGACACAG CCTGTGTGTG TGTGCTCAGC 
AGGAAGGCTA CAAGAAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosomal locations of the human and mouse genes for precursors of epidermal growth factor and the beta subunit of nerve growth factor.
Proceedings of the National Academy of Sciences of the United States of America82(2)469-73(1985 Jan)
Zabel BU,Eddy RL,Lalley PA,Scott J,Bell GI,Shows TB

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Ngf cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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