Klk1b4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klk1b4
Entrez Gene ID	18048
Full Name	kallikrein 1-related pepidase b4
Synonyms	Ngfa,alpha-NGF,mGk-4
General protein information	Preferred Names kallikrein 1-related pepidase b4 Names kallikrein 1-related peptidase-like b4 7S nerve growth factor alpha chain 7S nerve growth factor alpha subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B3\|7 28.72 cM
Summary	This gene encodes the alpha subunit of the 7S nerve growth factor (NGF) complex that is essential for the differentiation and survival of distinct populations of neurons in both the central and the peripheral nervous systems. This gene is located in a cluster of several related kallikrein genes on chromosome 7. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010915.4	NP_035045.2	kallikrein 1-related peptidase-like b4 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Klk1b5	NP_032482.1
Mus musculus (house mouse)	Klk1b1	NP_034775.1
Mus musculus (house mouse)	Klk1b3	NP_032719.1
Mus musculus (house mouse)	Klk1b24	NP_034773.1
Mus musculus (house mouse)	Klk1b26	NP_034774.1
Mus musculus (house mouse)	Klk1b4	NP_035045.2
Mus musculus (house mouse)	Klk1b22	NP_034244.1
Mus musculus (house mouse)	Klk1b8	NP_032483.1
Mus musculus (house mouse)	Klk1b21	NP_034772.1
Mus musculus (house mouse)	Klk1b27	NP_064664.1
Homo sapiens (human)	KLK3	NP_001639.1
Mus musculus (house mouse)	Klk1b16	NP_032480.1
Mus musculus (house mouse)	Klk1b11	NP_034770.1
	KLK3	NP_001036241.1
Mus musculus (house mouse)	Klk1	NP_034769.4
Mus musculus (house mouse)	Klk1b9	NP_034246.1

Gene Ontology
Bibliography

Related articles in PubMed

Music exposure differentially alters the levels of brain-derived neurotrophic factor and nerve growth factor in the mouse hypothalamus.
Angelucci F, Ricci E, Padua L, Sabino A, Tonali PA
Neuroscience letters429(2-3)152-5(2007 Dec)

Reduction in nerve growth factor availability leads to a conditioning lesion-like effect in sympathetic neurons.
Shoemaker SE, Sachs HH, Vaccariello SA, Zigmond RE
Journal of neurobiology66(12)1322-37(2006 Oct)

A comprehensive nomenclature for serine proteases with homology to tissue kallikreins.
Lundwall A, Band V, Blaber M, Clements JA, Courty Y, Diamandis EP, Fritz H, Lilja H, Malm J, Maltais LJ, Olsson AY, Petraki C, Scorilas A, Sotiropoulou G, Stenman UH, Stephan C, Talieri M, Yousef GM
Biological chemistry387(6)637-41(2006 Jun)

Neural driven angiogenesis by overexpression of nerve growth factor.
Hansen-Algenstaedt N, Algenstaedt P, Schaefer C, Hamann A, Wolfram L, Cing?z G, Kilic N, Schwarzloh B, Schroeder M, Joscheck C, Wiesner L, R?ther W, Erg?n S
Histochemistry and cell biology125(6)637-49(2006 Jun)

Local phosphatidylinositol 3,4,5-trisphosphate accumulation recruits Vav2 and Vav3 to activate Rac1/Cdc42 and initiate neurite outgrowth in nerve growth factor-stimulated PC12 cells.
Aoki K, Nakamura T, Fujikawa K, Matsuda M
Molecular biology of the cell16(5)2207-17(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exposure to music in mice can influence neurotrophin production in the hypothalamus. Physiological effects of music might be in part mediated by modulation of neurotrophins.
Title: Music exposure differentially alters the levels of brain-derived neurotrophic factor and nerve growth factor in the mouse hypothalamus.

The results suggest that the decreased availability of NGF after axotomy is a sufficient stimulus to induce the conditioning lesion effect in sympathetic neurons.
Title: Reduction in nerve growth factor availability leads to a conditioning lesion-like effect in sympathetic neurons.

We demonstrate that overexpression of nerve growth factor (NGF) in brown adipose tissue (BAT) of NGF-transgenic mice elevates both mRNA and protein levels of vascular endothelial growth factor (VEGF) and VEGF-receptors.
Title: Neural driven angiogenesis by overexpression of nerve growth factor.

NGF controls dendrite development in hippocampal neurons by binding to p75NTR and modulating the cellular targets of Notch
Title: NGF controls dendrite development in hippocampal neurons by binding to p75NTR and modulating the cellular targets of Notch.

Nerve growth factor is regulated by vasoactive intestinal peptide in the embryonic mouse.
Title: Vasoactive intestinal peptide regulation of nerve growth factor in the embryonic mouse.

The following Klk1b4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klk1b4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07151	NM_010915.4 Latest version!	Mus musculus kallikrein 1-related pepidase b4 (Klk1b4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klk1b4 ( NM_010915.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035045.2 Mus musculus kallikrein 1-related pepidase b4 (Klk1b4), mRNA.

Price & Availability↑

CloneID	OMu07151
Clone ID Related Accession (Same CDS sequence)	NM_010915.4
Accession Version	NM_010915.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	kallikrein 1-related peptidase-like b4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124176.3 and BF454440.1. On Feb 23, 2016 this sequence version replaced NM_010915.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGTTCC TGATCCTGTT CCTAGCCCTG TCCCTAGGAG GGATTGATGC TGCACCTCCT 
GTCCAGTCTC AAGTGGACTG TGAGAATTCC CAACCCTGGC ATGTGGCTGT GTACCGCTTC 
AACAAATATC AATGCGGGGG AGTCCTGTTG GACCGCAACT GGGTTCTCAC AGCTGCCCAC 
TGCTATAACG ACAAGTACCA GGTTTGGCTG GGCAAAAACA ACTTTTTGGA GGATGAACCT 
TCTGACCAAC ACCGGCTTGT CAGCAAAGCC ATCCCTCACC CTGACTTCAA CATGAGCCTC 
CTGAATGAGC ACACCCCACA ACCTGAGGAC GACTACAGCA ATGACCTGAT GCTGCTCCGC 
CTCAGCAAGC CTGCTGACAT CACAGATGTT GTGAAGCCCA TCACCCTGCC CACTGAGGAG 
CCCAAGCTGG GGAGCACATG CCTAGCCTCA GGCTGGGGCA GCACTACACC CATCAAGTTC 
AAATATCCAG ATGATCTCCA GTGTGTGAAC CTCAAGCTCC TGCCTAATGA GGACTGTGAC 
AAAGCACATG AAATGAAGGT GACAGATGCC ATGCTGTGTG CAGGAGAGAT GGATGGTGGC 
TCATACACTT GTGAGCATGA CTCAGGAGGC CCACTGATCT GTGATGGTAT TCTCCAAGGT 
ATCACATCAT GGGGCCCTGA ACCATGTGGT GAGCCCACTG AGCCAAGTGT CTACACCAAA 
CTTATTAAGT TCAGCTCCTG GATAAGAGAA ACTATGGCGA ATAACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035045.2
CDS	22..792
Translation	MWFLILFLALSLGGIDAAPPVQSQVDCENSQPWHVAVYRFNKYQCGGVLLDRNWVLTAAHCYNDKYQVWLGKNNFLEDEPSDQHRLVSKAIPHPDFNMSLLNEHTPQPEDDYSNDLMLLRLSKPADITDVVKPITLPTEEPKLGSTCLASGWGSTTPIKFKYPDDLQCVNLKLLPNEDCDKAHEMKVTDAMLCAGEMDGGSYTCEHDSGGPLICDGILQGITSWGPEPCGEPTEPSVYTKLIKFSSWIRETMANNP

Target ORF information:

RefSeq Version	NM_010915.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kallikrein 1-related pepidase b4 (Klk1b4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010915.4

ORF Insert Sequence:

ATGTGGTTCC TGATCCTGTT CCTAGCCCTG TCCCTAGGAG GGATTGATGC TGCACCTCCT 
GTCCAGTCTC AAGTGGACTG TGAGAATTCC CAACCCTGGC ATGTGGCTGT GTACCGCTTC 
AACAAATATC AATGCGGGGG AGTCCTGTTG GACCGCAACT GGGTTCTCAC AGCTGCCCAC 
TGCTATAACG ACAAGTACCA GGTTTGGCTG GGCAAAAACA ACTTTTTGGA GGATGAACCT 
TCTGACCAAC ACCGGCTTGT CAGCAAAGCC ATCCCTCACC CTGACTTCAA CATGAGCCTC 
CTGAATGAGC ACACCCCACA ACCTGAGGAC GACTACAGCA ATGACCTGAT GCTGCTCCGC 
CTCAGCAAGC CTGCTGACAT CACAGATGTT GTGAAGCCCA TCACCCTGCC CACTGAGGAG 
CCCAAGCTGG GGAGCACATG CCTAGCCTCA GGCTGGGGCA GCACTACACC CATCAAGTTC 
AAATATCCAG ATGATCTCCA GTGTGTGAAC CTCAAGCTCC TGCCTAATGA GGACTGTGAC 
AAAGCACATG AAATGAAGGT GACAGATGCC ATGCTGTGTG CAGGAGAGAT GGATGGTGGC 
TCATACACTT GTGAGCATGA CTCAGGAGGC CCACTGATCT GTGATGGTAT TCTCCAAGGT 
ATCACATCAT GGGGCCCTGA ACCATGTGGT GAGCCCACTG AGCCAAGTGT CTACACCAAA 
CTTATTAAGT TCAGCTCCTG GATAAGAGAA ACTATGGCGA ATAACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Music exposure differentially alters the levels of brain-derived neurotrophic factor and nerve growth factor in the mouse hypothalamus.
Neuroscience letters429(2-3)152-5(2007 Dec)
Angelucci F,Ricci E,Padua L,Sabino A,Tonali PA

Reduction in nerve growth factor availability leads to a conditioning lesion-like effect in sympathetic neurons.
Journal of neurobiology66(12)1322-37(2006 Oct)
Shoemaker SE,Sachs HH,Vaccariello SA,Zigmond RE

A comprehensive nomenclature for serine proteases with homology to tissue kallikreins.
Biological chemistry387(6)637-41(2006 Jun)
Lundwall A,Band V,Blaber M,Clements JA,Courty Y,Diamandis EP,Fritz H,Lilja H,Malm J,Maltais LJ,Olsson AY,Petraki C,Scorilas A,Sotiropoulou G,Stenman UH,Stephan C,Talieri M,Yousef GM

Neural driven angiogenesis by overexpression of nerve growth factor.
Histochemistry and cell biology125(6)637-49(2006 Jun)
Hansen-Algenstaedt N,Algenstaedt P,Schaefer C,Hamann A,Wolfram L,Cing?z G,Kilic N,Schwarzloh B,Schroeder M,Joscheck C,Wiesner L,R?ther W,Erg?n S

Local phosphatidylinositol 3,4,5-trisphosphate accumulation recruits Vav2 and Vav3 to activate Rac1/Cdc42 and initiate neurite outgrowth in nerve growth factor-stimulated PC12 cells.
Molecular biology of the cell16(5)2207-17(2005 May)
Aoki K,Nakamura T,Fujikawa K,Matsuda M

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Klk1b4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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