Nfkb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nfkb1
Entrez Gene ID	18033
Full Name	nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
Synonyms	NF-KB1,NF-kappaB,NF-kappaB1,p105,p50,p50/p105
General protein information	Preferred Names nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Names nuclear factor NF-kappa-B p105 subunit DNA-binding factor KBF1 EBP-1 NF kappaB1 NF-kappa-B1 p84/NF-kappa-B1 p98 NF-kappaB p50 nuclear factor kappaB p50 nuclear factor of kappa light chain gene enhancer in B-cells 1, p105 p50 subunit of NF kappaB
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 G3\|3 62.82 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501106.2	XP_006501169.1	nuclear factor NF-kappa-B p105 subunit isoform X1
XM_006501107.2	XP_006501170.1	nuclear factor NF-kappa-B p105 subunit isoform X2
NM_008689.2	NP_032715.2	nuclear factor NF-kappa-B p105 subunit
XM_006501107.1	XP_006501170.1	nuclear factor NF-kappa-B p105 subunit isoform X2
XM_006501106.3	XP_006501169.1	nuclear factor NF-kappa-B p105 subunit isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFKB1	XP_001168718.1
Canis lupus familiaris (dog)	NFKB1	NP_001003344.1
Bos taurus (cattle)	NFKB1	NP_001069877.1
Rattus norvegicus (Norway rat)	Nfkb1	NP_001263640.1
Gallus gallus (chicken)	NFKB1	NP_990465.1
Mus musculus (house mouse)	Nfkb1	NP_032715.2
Homo sapiens (human)	NFKB1	NP_003989.2
Xenopus tropicalis (tropical clawed frog)	nfkb1	XP_002934805.2
	NFKB1	XP_002804199.1

Gene Ontology

The following Nfkb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfkb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20734	NM_008689.2 Latest version!	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu31686	XM_006501106.3 Latest version!	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu31687	XM_006501107.2 Latest version!	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu31687	XM_006501107.1	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu31686	XM_006501106.2	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfkb1 ( NM_008689.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032715.2 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), mRNA.

Price & Availability↑

CloneID	OMu20734
Clone ID Related Accession (Same CDS sequence)	NM_008689.2
Accession Version	NM_008689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	nuclear factor NF-kappa-B p105 subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC050841.1, AB221614.1, AK159423.1 and AW907469.1. On Nov 8, 2006 this sequence version replaced NM_008689.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M57999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGACG ATGATCCCTA CGGAACTGGG CAAATGTTTC ATTTGAACAC TGCTTTGACT 
CACTCAATAT TTAATGCAGA ATTATATTCA CCAGAAATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TATTAGAGCA ACCAAAACAG AGGGGATTTC GATTCCGCTA TGTGTGTGAA 
GGCCCATCAC ACGGAGGGCT TCCGGGAGCC TCTAGTGAGA AGAACAAGAA ATCCTACCCA 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAACA TCCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGGGTA 
TGCACCGTAA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CATGTGACTA AGAAAAAGGT ATTTGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATT 
AGGGGCTATA ATCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA AGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCCGTGCAG 
CAGACCAAGG AGATGGACCT GAGCGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCTGAC 
AGCACTGGCA GCTTCACTCG GAGACTGGAG CCTGTGGTGT CAGACGCCAT CTATGATAGC 
AAAGCCCCGA ATGCATCCAA CCTGAAAATC GTGAGAATGG ACAGAACAGC AGGATGTGTG 
ACGGGAGGGG AGGAGATTTA CCTTCTCTGT GACAAGGTTC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGCGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACAGATGTTC ATAGACAGTT TGCCATTGTC TTCAAAACGC CAAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGGA AATCAGACCT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCTGAA ATCAAAGACA AAGAGGAAGT GCAAAGGAAA 
CGCCAGAAGC TTATGCCGAA CTTCTCGGAC AGCTTCGGTG GCGGCAGTGG AGCGGGAGCC 
GGTGGTGGAG GCATGTTCGG TAGTGGCGGT GGCGGAGGGA GTACCGGAAG CCCTGGCCCA 
GGGTATGGCT ACTCGAACTA CGGATTTCCT CCCTACGGTG GGATTACATT CCATCCCGGA 
GTCACGAAAT CCAACGCAGG GGTCACCCAT GGCACCATAA ACACCAAATT TAAAAATGGC 
CCTAAAGATT GTGCCAAGAG TGATGACGAG GAGAGTCTGA CTCTCCCTGA GAAGGAAACT 
GAAGGTGAAG GGCCCAGCCT GCCCATGGCC TGCACCAAGA CGGAACCCAT CGCCTTGGCA 
TCCACCATGG AAGACAAGGA GCAGGACATG GGATTTCAGG ATAACCTCTT TCTTGAGAAG 
GCTCTGCAGC TCGCCAGGCG ACACGCCAAC GCCCTTTTCG ACTACGCAGT GACGGGGGAT 
GTGAAGATGT TGCTGGCCGT GCAACGCCAT CTCACCGCCG TGCAGGATGA GAATGGGGAC 
AGTGTCTTAC ACTTAGCCAT CATCCACCTC CACGCTCAGC TTGTGAGGGA TCTGCTGGAA 
GTCACATCTG GTTTGATCTC TGATGACATC ATCAACATGA GAAATGACCT GTATCAGACA 
CCTCTGCACT TGGCCGTGAT CACCAAGCAG GAAGATGTAG TAGAGGATTT GCTGAGGGTT 
GGGGCTGACC TGAGCCTTCT GGACCGCTGG GGCAACTCTG TCCTGCACCT AGCTGCCAAA 
GAAGGACACG ACAGAATCCT CAGCATCCTG CTCAAGAGCA GAAAAGCAGC GCCCCTTATC 
GACCACCCCA ATGGGGAAGG TCTAAATGCC ATCCACATAG CTGTGATGAG CAATAGCCTG 
CCATGTCTGC TGCTGCTGGT GGCTGCCGGG GCAGAAGTCA ATGCTCAGGA GCAGAAGTCT 
GGGCGCACAG CGCTGCACCT GGCCGTGGAG TACGACAACA TCTCCTTGGC TGGCTGCCTG 
CTTCTGGAGG GTGATGCCCA CGTGGACAGT ACCACCTATG ATGGGACTAC ACCTCTGCAT 
ATAGCGGCCG GAAGAGGGTC CACCAGACTG GCAGCTCTTC TCAAAGCAGC AGGAGCAGAC 
CCCCTGGTGG AGAACTTTGA GCCTCTCTAT GACCTGGACG ACTCTTGGGA GAAGGCTGGA 
GAAGATGAGG GAGTGGTGCC AGGTACCACA CCCCTGGACA TGGCTGCCAA CTGGCAGGTA 
TTTGACATAC TAAATGGGAA ACCGTATGAG CCTGTGTTCA CATCTGATGA TATACTACCA 
CAAGGGGACA TGAAGCAGCT GACAGAAGAC ACGAGGCTAC AACTCTGCAA ACTGCTGGAA 
ATTCCTGATC CAGACAAAAA CTGGGCCACT CTGGCACAGA AGTTGGGTCT GGGGATACTG 
AACAATGCCT TCCGGCTGAG TCCTGCTCCT TCTAAAACTC TCATGGACAA CTATGAGGTC 
TCTGGGGGTA CCATCAAAGA GCTGATGGAG GCCCTGCAAC AGATGGGCTA CACAGAGGCC 
ATTGAAGTGA TCCAGGCAGC CTTCCGCACC CCGGCAACCA CAGCCTCCAG CCCCGTGACC 
ACTGCTCAGG TCCACTGTCT GCCTCTCTCG TCTTCCTCCA CGAGGCAGCA CATAGATGAA 
CTCCGGGATA GTGACAGCGT CTGTGACAGT GGTGTGGAGA CATCCTTCCG CAAACTCAGC 
TTTACAGAGT CTCTTACTGG AGACAGCCCA CTGCTATCTC TGAACAAAAT GCCCCACGGT 
TATGGGCAGG AAGGACCTAT TGAAGGCAAA ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032715.2
CDS	503..3418
Translation	MADDDPYGTGQMFHLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGVCTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTLPEKETEGEGPSLPMACTKTEPIALASTMEDKEQDMGFQDNLFLEKALQLARRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDRILSILLKSRKAAPLIDHPNGEGLNAIHIAVMSNSLPCLLLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTIKELMEALQQMGYTEAIEVIQAAFRTPATTASSPVTTAQVHCLPLSSSSTRQHIDELRDSDSVCDSGVETSFRKLSFTESLTGDSPLLSLNKMPHGYGQEGPIEGKI

Target ORF information:

RefSeq Version	NM_008689.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008689.2

ORF Insert Sequence:

ATGGCAGACG ATGATCCCTA CGGAACTGGG CAAATGTTTC ATTTGAACAC TGCTTTGACT 
CACTCAATAT TTAATGCAGA ATTATATTCA CCAGAAATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TATTAGAGCA ACCAAAACAG AGGGGATTTC GATTCCGCTA TGTGTGTGAA 
GGCCCATCAC ACGGAGGGCT TCCGGGAGCC TCTAGTGAGA AGAACAAGAA ATCCTACCCA 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAACA TCCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGGGTA 
TGCACCGTAA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CATGTGACTA AGAAAAAGGT ATTTGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATT 
AGGGGCTATA ATCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA AGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCCGTGCAG 
CAGACCAAGG AGATGGACCT GAGCGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCTGAC 
AGCACTGGCA GCTTCACTCG GAGACTGGAG CCTGTGGTGT CAGACGCCAT CTATGATAGC 
AAAGCCCCGA ATGCATCCAA CCTGAAAATC GTGAGAATGG ACAGAACAGC AGGATGTGTG 
ACGGGAGGGG AGGAGATTTA CCTTCTCTGT GACAAGGTTC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGCGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACAGATGTTC ATAGACAGTT TGCCATTGTC TTCAAAACGC CAAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGGA AATCAGACCT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCTGAA ATCAAAGACA AAGAGGAAGT GCAAAGGAAA 
CGCCAGAAGC TTATGCCGAA CTTCTCGGAC AGCTTCGGTG GCGGCAGTGG AGCGGGAGCC 
GGTGGTGGAG GCATGTTCGG TAGTGGCGGT GGCGGAGGGA GTACCGGAAG CCCTGGCCCA 
GGGTATGGCT ACTCGAACTA CGGATTTCCT CCCTACGGTG GGATTACATT CCATCCCGGA 
GTCACGAAAT CCAACGCAGG GGTCACCCAT GGCACCATAA ACACCAAATT TAAAAATGGC 
CCTAAAGATT GTGCCAAGAG TGATGACGAG GAGAGTCTGA CTCTCCCTGA GAAGGAAACT 
GAAGGTGAAG GGCCCAGCCT GCCCATGGCC TGCACCAAGA CGGAACCCAT CGCCTTGGCA 
TCCACCATGG AAGACAAGGA GCAGGACATG GGATTTCAGG ATAACCTCTT TCTTGAGAAG 
GCTCTGCAGC TCGCCAGGCG ACACGCCAAC GCCCTTTTCG ACTACGCAGT GACGGGGGAT 
GTGAAGATGT TGCTGGCCGT GCAACGCCAT CTCACCGCCG TGCAGGATGA GAATGGGGAC 
AGTGTCTTAC ACTTAGCCAT CATCCACCTC CACGCTCAGC TTGTGAGGGA TCTGCTGGAA 
GTCACATCTG GTTTGATCTC TGATGACATC ATCAACATGA GAAATGACCT GTATCAGACA 
CCTCTGCACT TGGCCGTGAT CACCAAGCAG GAAGATGTAG TAGAGGATTT GCTGAGGGTT 
GGGGCTGACC TGAGCCTTCT GGACCGCTGG GGCAACTCTG TCCTGCACCT AGCTGCCAAA 
GAAGGACACG ACAGAATCCT CAGCATCCTG CTCAAGAGCA GAAAAGCAGC GCCCCTTATC 
GACCACCCCA ATGGGGAAGG TCTAAATGCC ATCCACATAG CTGTGATGAG CAATAGCCTG 
CCATGTCTGC TGCTGCTGGT GGCTGCCGGG GCAGAAGTCA ATGCTCAGGA GCAGAAGTCT 
GGGCGCACAG CGCTGCACCT GGCCGTGGAG TACGACAACA TCTCCTTGGC TGGCTGCCTG 
CTTCTGGAGG GTGATGCCCA CGTGGACAGT ACCACCTATG ATGGGACTAC ACCTCTGCAT 
ATAGCGGCCG GAAGAGGGTC CACCAGACTG GCAGCTCTTC TCAAAGCAGC AGGAGCAGAC 
CCCCTGGTGG AGAACTTTGA GCCTCTCTAT GACCTGGACG ACTCTTGGGA GAAGGCTGGA 
GAAGATGAGG GAGTGGTGCC AGGTACCACA CCCCTGGACA TGGCTGCCAA CTGGCAGGTA 
TTTGACATAC TAAATGGGAA ACCGTATGAG CCTGTGTTCA CATCTGATGA TATACTACCA 
CAAGGGGACA TGAAGCAGCT GACAGAAGAC ACGAGGCTAC AACTCTGCAA ACTGCTGGAA 
ATTCCTGATC CAGACAAAAA CTGGGCCACT CTGGCACAGA AGTTGGGTCT GGGGATACTG 
AACAATGCCT TCCGGCTGAG TCCTGCTCCT TCTAAAACTC TCATGGACAA CTATGAGGTC 
TCTGGGGGTA CCATCAAAGA GCTGATGGAG GCCCTGCAAC AGATGGGCTA CACAGAGGCC 
ATTGAAGTGA TCCAGGCAGC CTTCCGCACC CCGGCAACCA CAGCCTCCAG CCCCGTGACC 
ACTGCTCAGG TCCACTGTCT GCCTCTCTCG TCTTCCTCCA CGAGGCAGCA CATAGATGAA 
CTCCGGGATA GTGACAGCGT CTGTGACAGT GGTGTGGAGA CATCCTTCCG CAAACTCAGC 
TTTACAGAGT CTCTTACTGG AGACAGCCCA CTGCTATCTC TGAACAAAAT GCCCCACGGT 
TATGGGCAGG AAGGACCTAT TGAAGGCAAA ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkb1 ( XM_006501106.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501169.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31686
Clone ID Related Accession (Same CDS sequence)	XM_006501106.2 , XM_006501106.3
Accession Version	XM_006501106.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear factor NF-kappa-B p105 subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG ATGATCCCTA CGGAACTGGG CAAATGTTTC ATTTGAACAC TGCTTTGACT 
CACTCAATAT TTAATGCAGA ATTATATTCA CCAGAAATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TATTAGAGCA ACCAAAACAG AGGGGATTTC GATTCCGCTA TGTGTGTGAA 
GGCCCATCAC ACGGAGGGCT TCCGGGAGCC TCTAGTGAGA AGAACAAGAA ATCCTACCCA 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAACA TCCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGGGTA 
TGCACCGTAA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CATGTGACTA AGAAAAAGGT ATTTGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATT 
AGGGGCTATA ATCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA AGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCCGTGCAG 
CAGACCAAGG AGATGGACCT GAGCGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCTGAC 
AGCACTGGCA GCTTCACTCG GAGACTGGAG CCTGTGGTGT CAGACGCCAT CTATGATAGC 
AAAGCCCCGA ATGCATCCAA CCTGAAAATC GTGAGAATGG ACAGAACAGC AGGATGTGTG 
ACGGGAGGGG AGGAGATTTA CCTTCTCTGT GACAAGGTTC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGCGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACAGATGTTC ATAGACAGTT TGCCATTGTC TTCAAAACGC CAAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGGA AATCAGACCT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCTGAA ATCAAAGACA AAGAGGAAGT GCAAAGGAAA 
CGCCAGAAGC TTATGCCGAA CTTCTCGGAC AGCTTCGGTG GCGGCAGTGG AGCGGGAGCC 
GGTGGTGGAG GCATGTTCGG TAGTGGCGGT GGCGGAGGGA GTACCGGAAG CCCTGGCCCA 
GGGTATGGCT ACTCGAACTA CGGATTTCCT CCCTACGGTG GGATTACATT CCATCCCGGA 
GTCACGAAAT CCAACGCAGG GGTCACCCAT GGCACCATAA ACACCAAATT TAAAAATGGC 
CCTAAAGATT GTGCCAAGAG TGATGACGAG GAGAGTCTGA CTCTCCCTGA GAAGGAAACT 
GAAGGTGAAG GGCCCAGCCT GCCCATGGCC TGCACCAAGA CGGAACCCAT CGCCTTGGCA 
TCCACCATGG AAGACAAGGA GCAGGACATG GGATTTCAGG ATAACCTCTT TCTTGAGAAG 
GCTCTGCAGC TCGCCAGGCG ACACGCCAAC GCCCTTTTCG ACTACGCAGT GACGGGGGAT 
GTGAAGATGT TGCTGGCCGT GCAACGCCAT CTCACCGCCG TGCAGGATGA GAATGGGGAC 
AGTGTCTTAC ACTTAGCCAT CATCCACCTC CACGCTCAGC TTGTGAGGGA TCTGCTGGAA 
GTCACATCTG GTTTGATCTC TGATGACATC ATCAACATGA GAAATGACCT GTATCAGACA 
CCTCTGCACT TGGCCGTGAT CACCAAGCAG GAAGATGTAG TAGAGGATTT GCTGAGGGTT 
GGGGCTGACC TGAGCCTTCT GGACCGCTGG GGCAACTCTG TCCTGCACCT AGCTGCCAAA 
GAAGGACACG ACAGAATCCT CAGCATCCTG CTCAAGAGCA GAAAAGCAGC GCCCCTTATC 
GACCACCCCA ATGGGGAAGG TCTAAATGCC ATCCACATAG CTGTGATGAG CAATAGCCTG 
CCATGTCTGC TGCTGCTGGT GGCTGCCGGG GCAGAAGTCA ATGCTCAGGA GCAGAAGTCT 
GGGCGCACAG CGCTGCACCT GGCCGTGGAG TACGACAACA TCTCCTTGGC TGGCTGCCTG 
CTTCTGGAGG GTGATGCCCA CGTGGACAGT ACCACCTATG ATGGGACTAC ACCTCTGCAT 
ATAGCGGCCG GAAGAGGGTC CACCAGACTG GCAGCTCTTC TCAAAGCAGC AGGAGCAGAC 
CCCCTGGTGG AGAACTTTGA GCCTCTCTAT GACCTGGACG ACTCTTGGGA GAAGGCTGGA 
GAAGATGAGG GAGTGGTGCC AGGTACCACA CCCCTGGACA TGGCTGCCAA CTGGCAGGTA 
TTTGACATAC TAAATGGGAA ACCGTATGAG CCTGTGTTCA CATCTGATGA TATACTACCA 
CAAGGGGACA TGAAGCAGCT GACAGAAGAC ACGAGGCTAC AACTCTGCAA ACTGCTGGAA 
ATTCCTGATC CAGACAAAAA CTGGGCCACT CTGGCACAGA AGTTGGGTCT GGGGATACTG 
AACAATGCCT TCCGGCTGAG TCCTGCTCCT TCTAAAACTC TCATGGACAA CTATGAGATG 
AACTCCGGGA TAGTGACAGC GTCTGTGACA GTGGTGTGGA GACATCCTTC CGCAAACTCA 
GCTTTACAGA GTCTCTTACT GGAGACAGCC CACTGCTATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501169.1
CDS	938..3622
Translation	MADDDPYGTGQMFHLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGVCTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTLPEKETEGEGPSLPMACTKTEPIALASTMEDKEQDMGFQDNLFLEKALQLARRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDRILSILLKSRKAAPLIDHPNGEGLNAIHIAVMSNSLPCLLLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEMNSGIVTASVTVVWRHPSANSALQSLLLETAHCYL

Target ORF information:

RefSeq Version	XM_006501106.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501106.2

ORF Insert Sequence:

ATGGCAGACG ATGATCCCTA CGGAACTGGG CAAATGTTTC ATTTGAACAC TGCTTTGACT 
CACTCAATAT TTAATGCAGA ATTATATTCA CCAGAAATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TATTAGAGCA ACCAAAACAG AGGGGATTTC GATTCCGCTA TGTGTGTGAA 
GGCCCATCAC ACGGAGGGCT TCCGGGAGCC TCTAGTGAGA AGAACAAGAA ATCCTACCCA 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAACA TCCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGGGTA 
TGCACCGTAA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CATGTGACTA AGAAAAAGGT ATTTGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATT 
AGGGGCTATA ATCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA AGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCCGTGCAG 
CAGACCAAGG AGATGGACCT GAGCGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCTGAC 
AGCACTGGCA GCTTCACTCG GAGACTGGAG CCTGTGGTGT CAGACGCCAT CTATGATAGC 
AAAGCCCCGA ATGCATCCAA CCTGAAAATC GTGAGAATGG ACAGAACAGC AGGATGTGTG 
ACGGGAGGGG AGGAGATTTA CCTTCTCTGT GACAAGGTTC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGCGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACAGATGTTC ATAGACAGTT TGCCATTGTC TTCAAAACGC CAAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGGA AATCAGACCT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCTGAA ATCAAAGACA AAGAGGAAGT GCAAAGGAAA 
CGCCAGAAGC TTATGCCGAA CTTCTCGGAC AGCTTCGGTG GCGGCAGTGG AGCGGGAGCC 
GGTGGTGGAG GCATGTTCGG TAGTGGCGGT GGCGGAGGGA GTACCGGAAG CCCTGGCCCA 
GGGTATGGCT ACTCGAACTA CGGATTTCCT CCCTACGGTG GGATTACATT CCATCCCGGA 
GTCACGAAAT CCAACGCAGG GGTCACCCAT GGCACCATAA ACACCAAATT TAAAAATGGC 
CCTAAAGATT GTGCCAAGAG TGATGACGAG GAGAGTCTGA CTCTCCCTGA GAAGGAAACT 
GAAGGTGAAG GGCCCAGCCT GCCCATGGCC TGCACCAAGA CGGAACCCAT CGCCTTGGCA 
TCCACCATGG AAGACAAGGA GCAGGACATG GGATTTCAGG ATAACCTCTT TCTTGAGAAG 
GCTCTGCAGC TCGCCAGGCG ACACGCCAAC GCCCTTTTCG ACTACGCAGT GACGGGGGAT 
GTGAAGATGT TGCTGGCCGT GCAACGCCAT CTCACCGCCG TGCAGGATGA GAATGGGGAC 
AGTGTCTTAC ACTTAGCCAT CATCCACCTC CACGCTCAGC TTGTGAGGGA TCTGCTGGAA 
GTCACATCTG GTTTGATCTC TGATGACATC ATCAACATGA GAAATGACCT GTATCAGACA 
CCTCTGCACT TGGCCGTGAT CACCAAGCAG GAAGATGTAG TAGAGGATTT GCTGAGGGTT 
GGGGCTGACC TGAGCCTTCT GGACCGCTGG GGCAACTCTG TCCTGCACCT AGCTGCCAAA 
GAAGGACACG ACAGAATCCT CAGCATCCTG CTCAAGAGCA GAAAAGCAGC GCCCCTTATC 
GACCACCCCA ATGGGGAAGG TCTAAATGCC ATCCACATAG CTGTGATGAG CAATAGCCTG 
CCATGTCTGC TGCTGCTGGT GGCTGCCGGG GCAGAAGTCA ATGCTCAGGA GCAGAAGTCT 
GGGCGCACAG CGCTGCACCT GGCCGTGGAG TACGACAACA TCTCCTTGGC TGGCTGCCTG 
CTTCTGGAGG GTGATGCCCA CGTGGACAGT ACCACCTATG ATGGGACTAC ACCTCTGCAT 
ATAGCGGCCG GAAGAGGGTC CACCAGACTG GCAGCTCTTC TCAAAGCAGC AGGAGCAGAC 
CCCCTGGTGG AGAACTTTGA GCCTCTCTAT GACCTGGACG ACTCTTGGGA GAAGGCTGGA 
GAAGATGAGG GAGTGGTGCC AGGTACCACA CCCCTGGACA TGGCTGCCAA CTGGCAGGTA 
TTTGACATAC TAAATGGGAA ACCGTATGAG CCTGTGTTCA CATCTGATGA TATACTACCA 
CAAGGGGACA TGAAGCAGCT GACAGAAGAC ACGAGGCTAC AACTCTGCAA ACTGCTGGAA 
ATTCCTGATC CAGACAAAAA CTGGGCCACT CTGGCACAGA AGTTGGGTCT GGGGATACTG 
AACAATGCCT TCCGGCTGAG TCCTGCTCCT TCTAAAACTC TCATGGACAA CTATGAGATG 
AACTCCGGGA TAGTGACAGC GTCTGTGACA GTGGTGTGGA GACATCCTTC CGCAAACTCA 
GCTTTACAGA GTCTCTTACT GGAGACAGCC CACTGCTATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkb1 ( XM_006501107.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501170.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X2, mRNA.

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CloneID	OMu31687
Clone ID Related Accession (Same CDS sequence)	XM_006501107.2 , XM_006501107.1
Accession Version	XM_006501107.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear factor NF-kappa-B p105 subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTTG GCTTTGCAAA CCTGGGAATA CTTCATGTGA CTAAGAAAAA GGTATTTGAA 
ACACTGGAAG CACGGATGAC AGAGGCGTGT ATTAGGGGCT ATAATCCTGG ACTTCTGGTG 
CATTCTGACC TTGCCTATCT ACAAGCAGAA GGCGGAGGAG ACCGGCAACT CACAGACAGA 
GAGAAGGAGA TCATCCGCCA GGCAGCCGTG CAGCAGACCA AGGAGATGGA CCTGAGCGTG 
GTGCGCCTCA TGTTCACAGC CTTCCTCCCT GACAGCACTG GCAGCTTCAC TCGGAGACTG 
GAGCCTGTGG TGTCAGACGC CATCTATGAT AGCAAAGCCC CGAATGCATC CAACCTGAAA 
ATCGTGAGAA TGGACAGAAC AGCAGGATGT GTGACGGGAG GGGAGGAGAT TTACCTTCTC 
TGTGACAAGG TTCAGAAAGA TGACATCCAG ATTCGGTTTT ATGAAGAGGA AGAAAATGGC 
GGAGTTTGGG AAGGATTTGG GGACTTTTCC CCCACAGATG TTCATAGACA GTTTGCCATT 
GTCTTCAAAA CGCCAAAGTA TAAGGATGTC AACATTACAA AGCCAGCTTC CGTGTTTGTT 
CAGCTTCGGA GGAAATCAGA CCTGGAAACT AGTGAACCGA AACCCTTTCT CTACTACCCT 
GAAATCAAAG ACAAAGAGGA AGTGCAAAGG AAACGCCAGA AGCTTATGCC GAACTTCTCG 
GACAGCTTCG GTGGCGGCAG TGGAGCGGGA GCCGGTGGTG GAGGCATGTT CGGTAGTGGC 
GGTGGCGGAG GGAGTACCGG AAGCCCTGGC CCAGGGTATG GCTACTCGAA CTACGGATTT 
CCTCCCTACG GTGGGATTAC ATTCCATCCC GGAGTCACGA AATCCAACGC AGGGGTCACC 
CATGGCACCA TAAACACCAA ATTTAAAAAT GGCCCTAAAG ATTGTGCCAA GAGTGATGAC 
GAGGAGAGTC TGACTCTCCC TGAGAAGGAA ACTGAAGGTG AAGGGCCCAG CCTGCCCATG 
GCCTGCACCA AGACGGAACC CATCGCCTTG GCATCCACCA TGGAAGACAA GGAGCAGGAC 
ATGGGATTTC AGGATAACCT CTTTCTTGAG AAGGCTCTGC AGCTCGCCAG GCGACACGCC 
AACGCCCTTT TCGACTACGC AGTGACGGGG GATGTGAAGA TGTTGCTGGC CGTGCAACGC 
CATCTCACCG CCGTGCAGGA TGAGAATGGG GACAGTGTCT TACACTTAGC CATCATCCAC 
CTCCACGCTC AGCTTGTGAG GGATCTGCTG GAAGTCACAT CTGGTTTGAT CTCTGATGAC 
ATCATCAACA TGAGAAATGA CCTGTATCAG ACACCTCTGC ACTTGGCCGT GATCACCAAG 
CAGGAAGATG TAGTAGAGGA TTTGCTGAGG GTTGGGGCTG ACCTGAGCCT TCTGGACCGC 
TGGGGCAACT CTGTCCTGCA CCTAGCTGCC AAAGAAGGAC ACGACAGAAT CCTCAGCATC 
CTGCTCAAGA GCAGAAAAGC AGCGCCCCTT ATCGACCACC CCAATGGGGA AGGTCTAAAT 
GCCATCCACA TAGCTGTGAT GAGCAATAGC CTGCCATGTC TGCTGCTGCT GGTGGCTGCC 
GGGGCAGAAG TCAATGCTCA GGAGCAGAAG TCTGGGCGCA CAGCGCTGCA CCTGGCCGTG 
GAGTACGACA ACATCTCCTT GGCTGGCTGC CTGCTTCTGG AGGGTGATGC CCACGTGGAC 
AGTACCACCT ATGATGGGAC TACACCTCTG CATATAGCGG CCGGAAGAGG GTCCACCAGA 
CTGGCAGCTC TTCTCAAAGC AGCAGGAGCA GACCCCCTGG TGGAGAACTT TGAGCCTCTC 
TATGACCTGG ACGACTCTTG GGAGAAGGCT GGAGAAGATG AGGGAGTGGT GCCAGGTACC 
ACACCCCTGG ACATGGCTGC CAACTGGCAG GTATTTGACA TACTAAATGG GAAACCGTAT 
GAGCCTGTGT TCACATCTGA TGATATACTA CCACAAGGGG ACATGAAGCA GCTGACAGAA 
GACACGAGGC TACAACTCTG CAAACTGCTG GAAATTCCTG ATCCAGACAA AAACTGGGCC 
ACTCTGGCAC AGAAGTTGGG TCTGGGGATA CTGAACAATG CCTTCCGGCT GAGTCCTGCT 
CCTTCTAAAA CTCTCATGGA CAACTATGAG GTCTCTGGGG GTACCATCAA AGAGCTGATG 
GAGGCCCTGC AACAGATGGG CTACACAGAG GCCATTGAAG TGATCCAGGC AGCCTTCCGC 
ACCCCGGCAA CCACAGCCTC CAGCCCCGTG ACCACTGCTC AGGTCCACTG TCTGCCTCTC 
TCGTCTTCCT CCACGAGGCA GCACATAGAT GAACTCCGGG ATAGTGACAG CGTCTGTGAC 
AGTGGTGTGG AGACATCCTT CCGCAAACTC AGCTTTACAG AGTCTCTTAC TGGAGACAGC 
CCACTGCTAT CTCTGAACAA AATGCCCCAC GGTTATGGGC AGGAAGGACC TATTGAAGGC 
AAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501170.1
CDS	580..3108
Translation	MVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTLPEKETEGEGPSLPMACTKTEPIALASTMEDKEQDMGFQDNLFLEKALQLARRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDRILSILLKSRKAAPLIDHPNGEGLNAIHIAVMSNSLPCLLLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTIKELMEALQQMGYTEAIEVIQAAFRTPATTASSPVTTAQVHCLPLSSSSTRQHIDELRDSDSVCDSGVETSFRKLSFTESLTGDSPLLSLNKMPHGYGQEGPIEGKI

Target ORF information:

RefSeq Version	XM_006501107.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501107.1

ORF Insert Sequence:

ATGGTGGTTG GCTTTGCAAA CCTGGGAATA CTTCATGTGA CTAAGAAAAA GGTATTTGAA 
ACACTGGAAG CACGGATGAC AGAGGCGTGT ATTAGGGGCT ATAATCCTGG ACTTCTGGTG 
CATTCTGACC TTGCCTATCT ACAAGCAGAA GGCGGAGGAG ACCGGCAACT CACAGACAGA 
GAGAAGGAGA TCATCCGCCA GGCAGCCGTG CAGCAGACCA AGGAGATGGA CCTGAGCGTG 
GTGCGCCTCA TGTTCACAGC CTTCCTCCCT GACAGCACTG GCAGCTTCAC TCGGAGACTG 
GAGCCTGTGG TGTCAGACGC CATCTATGAT AGCAAAGCCC CGAATGCATC CAACCTGAAA 
ATCGTGAGAA TGGACAGAAC AGCAGGATGT GTGACGGGAG GGGAGGAGAT TTACCTTCTC 
TGTGACAAGG TTCAGAAAGA TGACATCCAG ATTCGGTTTT ATGAAGAGGA AGAAAATGGC 
GGAGTTTGGG AAGGATTTGG GGACTTTTCC CCCACAGATG TTCATAGACA GTTTGCCATT 
GTCTTCAAAA CGCCAAAGTA TAAGGATGTC AACATTACAA AGCCAGCTTC CGTGTTTGTT 
CAGCTTCGGA GGAAATCAGA CCTGGAAACT AGTGAACCGA AACCCTTTCT CTACTACCCT 
GAAATCAAAG ACAAAGAGGA AGTGCAAAGG AAACGCCAGA AGCTTATGCC GAACTTCTCG 
GACAGCTTCG GTGGCGGCAG TGGAGCGGGA GCCGGTGGTG GAGGCATGTT CGGTAGTGGC 
GGTGGCGGAG GGAGTACCGG AAGCCCTGGC CCAGGGTATG GCTACTCGAA CTACGGATTT 
CCTCCCTACG GTGGGATTAC ATTCCATCCC GGAGTCACGA AATCCAACGC AGGGGTCACC 
CATGGCACCA TAAACACCAA ATTTAAAAAT GGCCCTAAAG ATTGTGCCAA GAGTGATGAC 
GAGGAGAGTC TGACTCTCCC TGAGAAGGAA ACTGAAGGTG AAGGGCCCAG CCTGCCCATG 
GCCTGCACCA AGACGGAACC CATCGCCTTG GCATCCACCA TGGAAGACAA GGAGCAGGAC 
ATGGGATTTC AGGATAACCT CTTTCTTGAG AAGGCTCTGC AGCTCGCCAG GCGACACGCC 
AACGCCCTTT TCGACTACGC AGTGACGGGG GATGTGAAGA TGTTGCTGGC CGTGCAACGC 
CATCTCACCG CCGTGCAGGA TGAGAATGGG GACAGTGTCT TACACTTAGC CATCATCCAC 
CTCCACGCTC AGCTTGTGAG GGATCTGCTG GAAGTCACAT CTGGTTTGAT CTCTGATGAC 
ATCATCAACA TGAGAAATGA CCTGTATCAG ACACCTCTGC ACTTGGCCGT GATCACCAAG 
CAGGAAGATG TAGTAGAGGA TTTGCTGAGG GTTGGGGCTG ACCTGAGCCT TCTGGACCGC 
TGGGGCAACT CTGTCCTGCA CCTAGCTGCC AAAGAAGGAC ACGACAGAAT CCTCAGCATC 
CTGCTCAAGA GCAGAAAAGC AGCGCCCCTT ATCGACCACC CCAATGGGGA AGGTCTAAAT 
GCCATCCACA TAGCTGTGAT GAGCAATAGC CTGCCATGTC TGCTGCTGCT GGTGGCTGCC 
GGGGCAGAAG TCAATGCTCA GGAGCAGAAG TCTGGGCGCA CAGCGCTGCA CCTGGCCGTG 
GAGTACGACA ACATCTCCTT GGCTGGCTGC CTGCTTCTGG AGGGTGATGC CCACGTGGAC 
AGTACCACCT ATGATGGGAC TACACCTCTG CATATAGCGG CCGGAAGAGG GTCCACCAGA 
CTGGCAGCTC TTCTCAAAGC AGCAGGAGCA GACCCCCTGG TGGAGAACTT TGAGCCTCTC 
TATGACCTGG ACGACTCTTG GGAGAAGGCT GGAGAAGATG AGGGAGTGGT GCCAGGTACC 
ACACCCCTGG ACATGGCTGC CAACTGGCAG GTATTTGACA TACTAAATGG GAAACCGTAT 
GAGCCTGTGT TCACATCTGA TGATATACTA CCACAAGGGG ACATGAAGCA GCTGACAGAA 
GACACGAGGC TACAACTCTG CAAACTGCTG GAAATTCCTG ATCCAGACAA AAACTGGGCC 
ACTCTGGCAC AGAAGTTGGG TCTGGGGATA CTGAACAATG CCTTCCGGCT GAGTCCTGCT 
CCTTCTAAAA CTCTCATGGA CAACTATGAG GTCTCTGGGG GTACCATCAA AGAGCTGATG 
GAGGCCCTGC AACAGATGGG CTACACAGAG GCCATTGAAG TGATCCAGGC AGCCTTCCGC 
ACCCCGGCAA CCACAGCCTC CAGCCCCGTG ACCACTGCTC AGGTCCACTG TCTGCCTCTC 
TCGTCTTCCT CCACGAGGCA GCACATAGAT GAACTCCGGG ATAGTGACAG CGTCTGTGAC 
AGTGGTGTGG AGACATCCTT CCGCAAACTC AGCTTTACAG AGTCTCTTAC TGGAGACAGC 
CCACTGCTAT CTCTGAACAA AATGCCCCAC GGTTATGGGC AGGAAGGACC TATTGAAGGC 
AAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkb1 ( XM_006501106.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501169.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31686
Clone ID Related Accession (Same CDS sequence)	XM_006501106.2 , XM_006501106.3
Accession Version	XM_006501106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear factor NF-kappa-B p105 subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG ATGATCCCTA CGGAACTGGG CAAATGTTTC ATTTGAACAC TGCTTTGACT 
CACTCAATAT TTAATGCAGA ATTATATTCA CCAGAAATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TATTAGAGCA ACCAAAACAG AGGGGATTTC GATTCCGCTA TGTGTGTGAA 
GGCCCATCAC ACGGAGGGCT TCCGGGAGCC TCTAGTGAGA AGAACAAGAA ATCCTACCCA 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAACA TCCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGGGTA 
TGCACCGTAA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CATGTGACTA AGAAAAAGGT ATTTGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATT 
AGGGGCTATA ATCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA AGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCCGTGCAG 
CAGACCAAGG AGATGGACCT GAGCGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCTGAC 
AGCACTGGCA GCTTCACTCG GAGACTGGAG CCTGTGGTGT CAGACGCCAT CTATGATAGC 
AAAGCCCCGA ATGCATCCAA CCTGAAAATC GTGAGAATGG ACAGAACAGC AGGATGTGTG 
ACGGGAGGGG AGGAGATTTA CCTTCTCTGT GACAAGGTTC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGCGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACAGATGTTC ATAGACAGTT TGCCATTGTC TTCAAAACGC CAAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGGA AATCAGACCT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCTGAA ATCAAAGACA AAGAGGAAGT GCAAAGGAAA 
CGCCAGAAGC TTATGCCGAA CTTCTCGGAC AGCTTCGGTG GCGGCAGTGG AGCGGGAGCC 
GGTGGTGGAG GCATGTTCGG TAGTGGCGGT GGCGGAGGGA GTACCGGAAG CCCTGGCCCA 
GGGTATGGCT ACTCGAACTA CGGATTTCCT CCCTACGGTG GGATTACATT CCATCCCGGA 
GTCACGAAAT CCAACGCAGG GGTCACCCAT GGCACCATAA ACACCAAATT TAAAAATGGC 
CCTAAAGATT GTGCCAAGAG TGATGACGAG GAGAGTCTGA CTCTCCCTGA GAAGGAAACT 
GAAGGTGAAG GGCCCAGCCT GCCCATGGCC TGCACCAAGA CGGAACCCAT CGCCTTGGCA 
TCCACCATGG AAGACAAGGA GCAGGACATG GGATTTCAGG ATAACCTCTT TCTTGAGAAG 
GCTCTGCAGC TCGCCAGGCG ACACGCCAAC GCCCTTTTCG ACTACGCAGT GACGGGGGAT 
GTGAAGATGT TGCTGGCCGT GCAACGCCAT CTCACCGCCG TGCAGGATGA GAATGGGGAC 
AGTGTCTTAC ACTTAGCCAT CATCCACCTC CACGCTCAGC TTGTGAGGGA TCTGCTGGAA 
GTCACATCTG GTTTGATCTC TGATGACATC ATCAACATGA GAAATGACCT GTATCAGACA 
CCTCTGCACT TGGCCGTGAT CACCAAGCAG GAAGATGTAG TAGAGGATTT GCTGAGGGTT 
GGGGCTGACC TGAGCCTTCT GGACCGCTGG GGCAACTCTG TCCTGCACCT AGCTGCCAAA 
GAAGGACACG ACAGAATCCT CAGCATCCTG CTCAAGAGCA GAAAAGCAGC GCCCCTTATC 
GACCACCCCA ATGGGGAAGG TCTAAATGCC ATCCACATAG CTGTGATGAG CAATAGCCTG 
CCATGTCTGC TGCTGCTGGT GGCTGCCGGG GCAGAAGTCA ATGCTCAGGA GCAGAAGTCT 
GGGCGCACAG CGCTGCACCT GGCCGTGGAG TACGACAACA TCTCCTTGGC TGGCTGCCTG 
CTTCTGGAGG GTGATGCCCA CGTGGACAGT ACCACCTATG ATGGGACTAC ACCTCTGCAT 
ATAGCGGCCG GAAGAGGGTC CACCAGACTG GCAGCTCTTC TCAAAGCAGC AGGAGCAGAC 
CCCCTGGTGG AGAACTTTGA GCCTCTCTAT GACCTGGACG ACTCTTGGGA GAAGGCTGGA 
GAAGATGAGG GAGTGGTGCC AGGTACCACA CCCCTGGACA TGGCTGCCAA CTGGCAGGTA 
TTTGACATAC TAAATGGGAA ACCGTATGAG CCTGTGTTCA CATCTGATGA TATACTACCA 
CAAGGGGACA TGAAGCAGCT GACAGAAGAC ACGAGGCTAC AACTCTGCAA ACTGCTGGAA 
ATTCCTGATC CAGACAAAAA CTGGGCCACT CTGGCACAGA AGTTGGGTCT GGGGATACTG 
AACAATGCCT TCCGGCTGAG TCCTGCTCCT TCTAAAACTC TCATGGACAA CTATGAGATG 
AACTCCGGGA TAGTGACAGC GTCTGTGACA GTGGTGTGGA GACATCCTTC CGCAAACTCA 
GCTTTACAGA GTCTCTTACT GGAGACAGCC CACTGCTATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501169.1
CDS	925..3609
Translation	MADDDPYGTGQMFHLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGVCTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTLPEKETEGEGPSLPMACTKTEPIALASTMEDKEQDMGFQDNLFLEKALQLARRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDRILSILLKSRKAAPLIDHPNGEGLNAIHIAVMSNSLPCLLLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEMNSGIVTASVTVVWRHPSANSALQSLLLETAHCYL

Target ORF information:

RefSeq Version	XM_006501106.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501106.3

ORF Insert Sequence:

ATGGCAGACG ATGATCCCTA CGGAACTGGG CAAATGTTTC ATTTGAACAC TGCTTTGACT 
CACTCAATAT TTAATGCAGA ATTATATTCA CCAGAAATAC CACTGTCAAC AGATGGCCCA 
TACCTTCAAA TATTAGAGCA ACCAAAACAG AGGGGATTTC GATTCCGCTA TGTGTGTGAA 
GGCCCATCAC ACGGAGGGCT TCCGGGAGCC TCTAGTGAGA AGAACAAGAA ATCCTACCCA 
CAGGTCAAAA TTTGCAACTA TGTGGGGCCT GCAAAGGTTA TCGTTCAGTT GGTCACAAAT 
GGAAAAAACA TCCACCTGCA CGCCCACAGC CTGGTGGGCA AGCACTGTGA GGACGGGGTA 
TGCACCGTAA CAGCAGGACC CAAGGACATG GTGGTTGGCT TTGCAAACCT GGGAATACTT 
CATGTGACTA AGAAAAAGGT ATTTGAAACA CTGGAAGCAC GGATGACAGA GGCGTGTATT 
AGGGGCTATA ATCCTGGACT TCTGGTGCAT TCTGACCTTG CCTATCTACA AGCAGAAGGC 
GGAGGAGACC GGCAACTCAC AGACAGAGAG AAGGAGATCA TCCGCCAGGC AGCCGTGCAG 
CAGACCAAGG AGATGGACCT GAGCGTGGTG CGCCTCATGT TCACAGCCTT CCTCCCTGAC 
AGCACTGGCA GCTTCACTCG GAGACTGGAG CCTGTGGTGT CAGACGCCAT CTATGATAGC 
AAAGCCCCGA ATGCATCCAA CCTGAAAATC GTGAGAATGG ACAGAACAGC AGGATGTGTG 
ACGGGAGGGG AGGAGATTTA CCTTCTCTGT GACAAGGTTC AGAAAGATGA CATCCAGATT 
CGGTTTTATG AAGAGGAAGA AAATGGCGGA GTTTGGGAAG GATTTGGGGA CTTTTCCCCC 
ACAGATGTTC ATAGACAGTT TGCCATTGTC TTCAAAACGC CAAAGTATAA GGATGTCAAC 
ATTACAAAGC CAGCTTCCGT GTTTGTTCAG CTTCGGAGGA AATCAGACCT GGAAACTAGT 
GAACCGAAAC CCTTTCTCTA CTACCCTGAA ATCAAAGACA AAGAGGAAGT GCAAAGGAAA 
CGCCAGAAGC TTATGCCGAA CTTCTCGGAC AGCTTCGGTG GCGGCAGTGG AGCGGGAGCC 
GGTGGTGGAG GCATGTTCGG TAGTGGCGGT GGCGGAGGGA GTACCGGAAG CCCTGGCCCA 
GGGTATGGCT ACTCGAACTA CGGATTTCCT CCCTACGGTG GGATTACATT CCATCCCGGA 
GTCACGAAAT CCAACGCAGG GGTCACCCAT GGCACCATAA ACACCAAATT TAAAAATGGC 
CCTAAAGATT GTGCCAAGAG TGATGACGAG GAGAGTCTGA CTCTCCCTGA GAAGGAAACT 
GAAGGTGAAG GGCCCAGCCT GCCCATGGCC TGCACCAAGA CGGAACCCAT CGCCTTGGCA 
TCCACCATGG AAGACAAGGA GCAGGACATG GGATTTCAGG ATAACCTCTT TCTTGAGAAG 
GCTCTGCAGC TCGCCAGGCG ACACGCCAAC GCCCTTTTCG ACTACGCAGT GACGGGGGAT 
GTGAAGATGT TGCTGGCCGT GCAACGCCAT CTCACCGCCG TGCAGGATGA GAATGGGGAC 
AGTGTCTTAC ACTTAGCCAT CATCCACCTC CACGCTCAGC TTGTGAGGGA TCTGCTGGAA 
GTCACATCTG GTTTGATCTC TGATGACATC ATCAACATGA GAAATGACCT GTATCAGACA 
CCTCTGCACT TGGCCGTGAT CACCAAGCAG GAAGATGTAG TAGAGGATTT GCTGAGGGTT 
GGGGCTGACC TGAGCCTTCT GGACCGCTGG GGCAACTCTG TCCTGCACCT AGCTGCCAAA 
GAAGGACACG ACAGAATCCT CAGCATCCTG CTCAAGAGCA GAAAAGCAGC GCCCCTTATC 
GACCACCCCA ATGGGGAAGG TCTAAATGCC ATCCACATAG CTGTGATGAG CAATAGCCTG 
CCATGTCTGC TGCTGCTGGT GGCTGCCGGG GCAGAAGTCA ATGCTCAGGA GCAGAAGTCT 
GGGCGCACAG CGCTGCACCT GGCCGTGGAG TACGACAACA TCTCCTTGGC TGGCTGCCTG 
CTTCTGGAGG GTGATGCCCA CGTGGACAGT ACCACCTATG ATGGGACTAC ACCTCTGCAT 
ATAGCGGCCG GAAGAGGGTC CACCAGACTG GCAGCTCTTC TCAAAGCAGC AGGAGCAGAC 
CCCCTGGTGG AGAACTTTGA GCCTCTCTAT GACCTGGACG ACTCTTGGGA GAAGGCTGGA 
GAAGATGAGG GAGTGGTGCC AGGTACCACA CCCCTGGACA TGGCTGCCAA CTGGCAGGTA 
TTTGACATAC TAAATGGGAA ACCGTATGAG CCTGTGTTCA CATCTGATGA TATACTACCA 
CAAGGGGACA TGAAGCAGCT GACAGAAGAC ACGAGGCTAC AACTCTGCAA ACTGCTGGAA 
ATTCCTGATC CAGACAAAAA CTGGGCCACT CTGGCACAGA AGTTGGGTCT GGGGATACTG 
AACAATGCCT TCCGGCTGAG TCCTGCTCCT TCTAAAACTC TCATGGACAA CTATGAGATG 
AACTCCGGGA TAGTGACAGC GTCTGTGACA GTGGTGTGGA GACATCCTTC CGCAAACTCA 
GCTTTACAGA GTCTCTTACT GGAGACAGCC CACTGCTATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfkb1 ( XM_006501107.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501170.1 Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X2, mRNA.

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CloneID	OMu31687
Clone ID Related Accession (Same CDS sequence)	XM_006501107.2 , XM_006501107.1
Accession Version	XM_006501107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear factor NF-kappa-B p105 subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTTG GCTTTGCAAA CCTGGGAATA CTTCATGTGA CTAAGAAAAA GGTATTTGAA 
ACACTGGAAG CACGGATGAC AGAGGCGTGT ATTAGGGGCT ATAATCCTGG ACTTCTGGTG 
CATTCTGACC TTGCCTATCT ACAAGCAGAA GGCGGAGGAG ACCGGCAACT CACAGACAGA 
GAGAAGGAGA TCATCCGCCA GGCAGCCGTG CAGCAGACCA AGGAGATGGA CCTGAGCGTG 
GTGCGCCTCA TGTTCACAGC CTTCCTCCCT GACAGCACTG GCAGCTTCAC TCGGAGACTG 
GAGCCTGTGG TGTCAGACGC CATCTATGAT AGCAAAGCCC CGAATGCATC CAACCTGAAA 
ATCGTGAGAA TGGACAGAAC AGCAGGATGT GTGACGGGAG GGGAGGAGAT TTACCTTCTC 
TGTGACAAGG TTCAGAAAGA TGACATCCAG ATTCGGTTTT ATGAAGAGGA AGAAAATGGC 
GGAGTTTGGG AAGGATTTGG GGACTTTTCC CCCACAGATG TTCATAGACA GTTTGCCATT 
GTCTTCAAAA CGCCAAAGTA TAAGGATGTC AACATTACAA AGCCAGCTTC CGTGTTTGTT 
CAGCTTCGGA GGAAATCAGA CCTGGAAACT AGTGAACCGA AACCCTTTCT CTACTACCCT 
GAAATCAAAG ACAAAGAGGA AGTGCAAAGG AAACGCCAGA AGCTTATGCC GAACTTCTCG 
GACAGCTTCG GTGGCGGCAG TGGAGCGGGA GCCGGTGGTG GAGGCATGTT CGGTAGTGGC 
GGTGGCGGAG GGAGTACCGG AAGCCCTGGC CCAGGGTATG GCTACTCGAA CTACGGATTT 
CCTCCCTACG GTGGGATTAC ATTCCATCCC GGAGTCACGA AATCCAACGC AGGGGTCACC 
CATGGCACCA TAAACACCAA ATTTAAAAAT GGCCCTAAAG ATTGTGCCAA GAGTGATGAC 
GAGGAGAGTC TGACTCTCCC TGAGAAGGAA ACTGAAGGTG AAGGGCCCAG CCTGCCCATG 
GCCTGCACCA AGACGGAACC CATCGCCTTG GCATCCACCA TGGAAGACAA GGAGCAGGAC 
ATGGGATTTC AGGATAACCT CTTTCTTGAG AAGGCTCTGC AGCTCGCCAG GCGACACGCC 
AACGCCCTTT TCGACTACGC AGTGACGGGG GATGTGAAGA TGTTGCTGGC CGTGCAACGC 
CATCTCACCG CCGTGCAGGA TGAGAATGGG GACAGTGTCT TACACTTAGC CATCATCCAC 
CTCCACGCTC AGCTTGTGAG GGATCTGCTG GAAGTCACAT CTGGTTTGAT CTCTGATGAC 
ATCATCAACA TGAGAAATGA CCTGTATCAG ACACCTCTGC ACTTGGCCGT GATCACCAAG 
CAGGAAGATG TAGTAGAGGA TTTGCTGAGG GTTGGGGCTG ACCTGAGCCT TCTGGACCGC 
TGGGGCAACT CTGTCCTGCA CCTAGCTGCC AAAGAAGGAC ACGACAGAAT CCTCAGCATC 
CTGCTCAAGA GCAGAAAAGC AGCGCCCCTT ATCGACCACC CCAATGGGGA AGGTCTAAAT 
GCCATCCACA TAGCTGTGAT GAGCAATAGC CTGCCATGTC TGCTGCTGCT GGTGGCTGCC 
GGGGCAGAAG TCAATGCTCA GGAGCAGAAG TCTGGGCGCA CAGCGCTGCA CCTGGCCGTG 
GAGTACGACA ACATCTCCTT GGCTGGCTGC CTGCTTCTGG AGGGTGATGC CCACGTGGAC 
AGTACCACCT ATGATGGGAC TACACCTCTG CATATAGCGG CCGGAAGAGG GTCCACCAGA 
CTGGCAGCTC TTCTCAAAGC AGCAGGAGCA GACCCCCTGG TGGAGAACTT TGAGCCTCTC 
TATGACCTGG ACGACTCTTG GGAGAAGGCT GGAGAAGATG AGGGAGTGGT GCCAGGTACC 
ACACCCCTGG ACATGGCTGC CAACTGGCAG GTATTTGACA TACTAAATGG GAAACCGTAT 
GAGCCTGTGT TCACATCTGA TGATATACTA CCACAAGGGG ACATGAAGCA GCTGACAGAA 
GACACGAGGC TACAACTCTG CAAACTGCTG GAAATTCCTG ATCCAGACAA AAACTGGGCC 
ACTCTGGCAC AGAAGTTGGG TCTGGGGATA CTGAACAATG CCTTCCGGCT GAGTCCTGCT 
CCTTCTAAAA CTCTCATGGA CAACTATGAG GTCTCTGGGG GTACCATCAA AGAGCTGATG 
GAGGCCCTGC AACAGATGGG CTACACAGAG GCCATTGAAG TGATCCAGGC AGCCTTCCGC 
ACCCCGGCAA CCACAGCCTC CAGCCCCGTG ACCACTGCTC AGGTCCACTG TCTGCCTCTC 
TCGTCTTCCT CCACGAGGCA GCACATAGAT GAACTCCGGG ATAGTGACAG CGTCTGTGAC 
AGTGGTGTGG AGACATCCTT CCGCAAACTC AGCTTTACAG AGTCTCTTAC TGGAGACAGC 
CCACTGCTAT CTCTGAACAA AATGCCCCAC GGTTATGGGC AGGAAGGACC TATTGAAGGC 
AAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501170.1
CDS	604..3132
Translation	MVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTLPEKETEGEGPSLPMACTKTEPIALASTMEDKEQDMGFQDNLFLEKALQLARRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDRILSILLKSRKAAPLIDHPNGEGLNAIHIAVMSNSLPCLLLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTIKELMEALQQMGYTEAIEVIQAAFRTPATTASSPVTTAQVHCLPLSSSSTRQHIDELRDSDSVCDSGVETSFRKLSFTESLTGDSPLLSLNKMPHGYGQEGPIEGKI

Target ORF information:

RefSeq Version	XM_006501107.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501107.2

ORF Insert Sequence:

ATGGTGGTTG GCTTTGCAAA CCTGGGAATA CTTCATGTGA CTAAGAAAAA GGTATTTGAA 
ACACTGGAAG CACGGATGAC AGAGGCGTGT ATTAGGGGCT ATAATCCTGG ACTTCTGGTG 
CATTCTGACC TTGCCTATCT ACAAGCAGAA GGCGGAGGAG ACCGGCAACT CACAGACAGA 
GAGAAGGAGA TCATCCGCCA GGCAGCCGTG CAGCAGACCA AGGAGATGGA CCTGAGCGTG 
GTGCGCCTCA TGTTCACAGC CTTCCTCCCT GACAGCACTG GCAGCTTCAC TCGGAGACTG 
GAGCCTGTGG TGTCAGACGC CATCTATGAT AGCAAAGCCC CGAATGCATC CAACCTGAAA 
ATCGTGAGAA TGGACAGAAC AGCAGGATGT GTGACGGGAG GGGAGGAGAT TTACCTTCTC 
TGTGACAAGG TTCAGAAAGA TGACATCCAG ATTCGGTTTT ATGAAGAGGA AGAAAATGGC 
GGAGTTTGGG AAGGATTTGG GGACTTTTCC CCCACAGATG TTCATAGACA GTTTGCCATT 
GTCTTCAAAA CGCCAAAGTA TAAGGATGTC AACATTACAA AGCCAGCTTC CGTGTTTGTT 
CAGCTTCGGA GGAAATCAGA CCTGGAAACT AGTGAACCGA AACCCTTTCT CTACTACCCT 
GAAATCAAAG ACAAAGAGGA AGTGCAAAGG AAACGCCAGA AGCTTATGCC GAACTTCTCG 
GACAGCTTCG GTGGCGGCAG TGGAGCGGGA GCCGGTGGTG GAGGCATGTT CGGTAGTGGC 
GGTGGCGGAG GGAGTACCGG AAGCCCTGGC CCAGGGTATG GCTACTCGAA CTACGGATTT 
CCTCCCTACG GTGGGATTAC ATTCCATCCC GGAGTCACGA AATCCAACGC AGGGGTCACC 
CATGGCACCA TAAACACCAA ATTTAAAAAT GGCCCTAAAG ATTGTGCCAA GAGTGATGAC 
GAGGAGAGTC TGACTCTCCC TGAGAAGGAA ACTGAAGGTG AAGGGCCCAG CCTGCCCATG 
GCCTGCACCA AGACGGAACC CATCGCCTTG GCATCCACCA TGGAAGACAA GGAGCAGGAC 
ATGGGATTTC AGGATAACCT CTTTCTTGAG AAGGCTCTGC AGCTCGCCAG GCGACACGCC 
AACGCCCTTT TCGACTACGC AGTGACGGGG GATGTGAAGA TGTTGCTGGC CGTGCAACGC 
CATCTCACCG CCGTGCAGGA TGAGAATGGG GACAGTGTCT TACACTTAGC CATCATCCAC 
CTCCACGCTC AGCTTGTGAG GGATCTGCTG GAAGTCACAT CTGGTTTGAT CTCTGATGAC 
ATCATCAACA TGAGAAATGA CCTGTATCAG ACACCTCTGC ACTTGGCCGT GATCACCAAG 
CAGGAAGATG TAGTAGAGGA TTTGCTGAGG GTTGGGGCTG ACCTGAGCCT TCTGGACCGC 
TGGGGCAACT CTGTCCTGCA CCTAGCTGCC AAAGAAGGAC ACGACAGAAT CCTCAGCATC 
CTGCTCAAGA GCAGAAAAGC AGCGCCCCTT ATCGACCACC CCAATGGGGA AGGTCTAAAT 
GCCATCCACA TAGCTGTGAT GAGCAATAGC CTGCCATGTC TGCTGCTGCT GGTGGCTGCC 
GGGGCAGAAG TCAATGCTCA GGAGCAGAAG TCTGGGCGCA CAGCGCTGCA CCTGGCCGTG 
GAGTACGACA ACATCTCCTT GGCTGGCTGC CTGCTTCTGG AGGGTGATGC CCACGTGGAC 
AGTACCACCT ATGATGGGAC TACACCTCTG CATATAGCGG CCGGAAGAGG GTCCACCAGA 
CTGGCAGCTC TTCTCAAAGC AGCAGGAGCA GACCCCCTGG TGGAGAACTT TGAGCCTCTC 
TATGACCTGG ACGACTCTTG GGAGAAGGCT GGAGAAGATG AGGGAGTGGT GCCAGGTACC 
ACACCCCTGG ACATGGCTGC CAACTGGCAG GTATTTGACA TACTAAATGG GAAACCGTAT 
GAGCCTGTGT TCACATCTGA TGATATACTA CCACAAGGGG ACATGAAGCA GCTGACAGAA 
GACACGAGGC TACAACTCTG CAAACTGCTG GAAATTCCTG ATCCAGACAA AAACTGGGCC 
ACTCTGGCAC AGAAGTTGGG TCTGGGGATA CTGAACAATG CCTTCCGGCT GAGTCCTGCT 
CCTTCTAAAA CTCTCATGGA CAACTATGAG GTCTCTGGGG GTACCATCAA AGAGCTGATG 
GAGGCCCTGC AACAGATGGG CTACACAGAG GCCATTGAAG TGATCCAGGC AGCCTTCCGC 
ACCCCGGCAA CCACAGCCTC CAGCCCCGTG ACCACTGCTC AGGTCCACTG TCTGCCTCTC 
TCGTCTTCCT CCACGAGGCA GCACATAGAT GAACTCCGGG ATAGTGACAG CGTCTGTGAC 
AGTGGTGTGG AGACATCCTT CCGCAAACTC AGCTTTACAG AGTCTCTTAC TGGAGACAGC 
CCACTGCTAT CTCTGAACAA AATGCCCCAC GGTTATGGGC AGGAAGGACC TATTGAAGGC 
AAAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Nfkb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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