Nedd8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nedd8
Entrez Gene ID	18002
Full Name	neural precursor cell expressed, developmentally down-regulated gene 8
Synonyms	Rub1
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated gene 8 Names NEDD8 NEDD-8 neddylin neural precursor cell expressed developmentally down-regulated protein 8 ubiquitin-like protein Nedd8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 C3\|14 28.19 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008683.3	NP_032709.1	NEDD8 precursor
NM_008683.4	NP_032709.1	NEDD8 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NEDD8	XP_537389.2
Rattus norvegicus (Norway rat)	Nedd8	NP_620233.1
Drosophila melanogaster (fruit fly)	Nedd8	NP_609919.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007913	XP_317573.3
Saccharomyces cerevisiae (baker's yeast)	RUB1	NP_010423.4
Schizosaccharomyces pombe (fission yeast)	ned8	NP_595955.1
Magnaporthe oryzae (rice blast fungus)	MGG_09887	XP_003709980.1
	NEDD8	XP_001113390.1
Mus musculus (house mouse)	Nedd8	NP_032709.1
Homo sapiens (human)	NEDD8	NP_006147.1
Pan troglodytes (chimpanzee)	NEDD8	XP_003952499.1
Caenorhabditis elegans (roundworm)	ned-8	NP_492717.1
Kluyveromyces lactis	KLLA0F17974g	XP_455886.1
Eremothecium gossypii	AGOS_AGL185W	NP_986482.2
Bos taurus (cattle)	NEDD8	NP_777189.1
Danio rerio (zebrafish)	nedd8l	NP_001002557.1
Danio rerio (zebrafish)	nedd8	NP_958478.2
Neurospora crassa	NCU03309	XP_964486.1
Xenopus tropicalis (tropical clawed frog)	nedd8	NP_001016973.1

Gene Ontology
Bibliography

Related articles in PubMed

Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury.
Zhu Z, Sun L, Hao R, Jiang H, Qian F, Ye RD
American journal of physiology. Lung cellular and molecular physiology313(1)L104-L114(2017 Jul)

Promoting tumorigenesis in nasopharyngeal carcinoma, NEDD8 serves as a potential theranostic target.
Xie P, Yang JP, Cao Y, Peng LX, Zheng LS, Sun R, Meng DF, Wang MY, Mei Y, Qiang YY, Cao L, Xiang YQ, Luo DH, Yun JP, Huang BJ, Jia LJ, Qian CN
Cell death & disease8(6)e2834(2017 Jun)

An inhibitor of apoptosis protein antagonist T-3256336 potentiates the antitumor efficacy of the Nedd8-activating enzyme inhibitor pevonedistat (TAK-924/MLN4924).
Sumi H, Inazuka M, Morimoto M, Hibino R, Hashimoto K, Ishikawa T, Kuida K, Smith PG, Yoshida S, Yabuki M
Biochemical and biophysical research communications480(3)380-386(2016 Nov)

Identification of Novel Host Interactors of Effectors Secreted by
Sontag RL, Nakayasu ES, Brown RN, Niemann GS, Sydor MA, Sanchez O, Ansong C, Lu SY, Choi H, Valleau D, Weitz KK, Savchenko A, Cambronne ED, Adkins JN
mSystems1(4)(2016 Jul-Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

neural precursor cell expressed developmentally downregulated protein 8 (Nedd8) modification of Cul-5 is required for its interaction with TRAF6.
Title: Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury.

Our findings provide mechanistic evidence of the effects of IAP antagonists in combination with NAE inhibitors, and demonstrate the potential of a new combination therapy for cancer.
Title: An inhibitor of apoptosis protein antagonist T-3256336 potentiates the antitumor efficacy of the Nedd8-activating enzyme inhibitor pevonedistat (TAK-924/MLN4924).

Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity.
Title: NEDL2 regulates enteric nervous system and kidney development in its Nedd8 ligase activity-dependent manner.

a role of Nedd8 in regulating caspase-1 activation following inflammasome activation
Title: Nedd8 regulates inflammasome-dependent caspase-1 activation.

Nedd8 and its associated E2 carrier protein (Ubc12) and E3-SCF ligase play a key role in regulating the expression and activity of ENaC in alveolar pneumocytes.
Title: H2O2 regulates lung epithelial sodium channel (ENaC) via ubiquitin-like protein Nedd8.

The following Nedd8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18704	NM_008683.4 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu18704	NM_008683.3	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nedd8 ( NM_008683.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032709.1 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA.

Price & Availability↑

CloneID	OMu18704
Clone ID Related Accession (Same CDS sequence)	NM_008683.3 , NM_008683.4
Accession Version	NM_008683.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-09
Organism	Mus musculus(house mouse)
Product	NEDD8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK146732.1. On Apr 5, 2007 this sequence version replaced NM_008683.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146732.1, CJ228204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCTAATTA AAGTGAAGAC GCTGACTGGG AAGGAGATTG AGATAGACAT CGAACCCACA 
GACAAGGTGG AGCGAATCAA GGAGCGTGTG GAAGAAAAAG AAGGGATTCC CCCCCAGCAG 
CAGCGGCTCA TCTACAGTGG CAAGCAAATG AATGATGAGA AGACAGCAGC TGATTACAAG 
ATTCTAGGTG GTTCCGTCCT CCACCTGGTG TTGGCTCTTA GAGGAGGAGG TGGTCTTGGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032709.1
CDS	95..340
Translation	MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLGQ

Target ORF information:

RefSeq Version	NM_008683.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008683.3

ORF Insert Sequence:

ATGCTAATTA AAGTGAAGAC GCTGACTGGG AAGGAGATTG AGATAGACAT CGAACCCACA 
GACAAGGTGG AGCGAATCAA GGAGCGTGTG GAAGAAAAAG AAGGGATTCC CCCCCAGCAG 
CAGCGGCTCA TCTACAGTGG CAAGCAAATG AATGATGAGA AGACAGCAGC TGATTACAAG 
ATTCTAGGTG GTTCCGTCCT CCACCTGGTG TTGGCTCTTA GAGGAGGAGG TGGTCTTGGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd8 ( NM_008683.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032709.1 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA.

Price & Availability↑

CloneID	OMu18704
Clone ID Related Accession (Same CDS sequence)	NM_008683.3 , NM_008683.4
Accession Version	NM_008683.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Mus musculus(house mouse)
Product	NEDD8 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK146732.1 and AI847056.1. On Feb 5, 2019 this sequence version replaced NM_008683.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DV661605.1, SRR7345562.4007404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCTAATTA AAGTGAAGAC GCTGACTGGG AAGGAGATTG AGATAGACAT CGAACCCACA 
GACAAGGTGG AGCGAATCAA GGAGCGTGTG GAAGAAAAAG AAGGGATTCC CCCCCAGCAG 
CAGCGGCTCA TCTACAGTGG CAAGCAAATG AATGATGAGA AGACAGCAGC TGATTACAAG 
ATTCTAGGTG GTTCCGTCCT CCACCTGGTG TTGGCTCTTA GAGGAGGAGG TGGTCTTGGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032709.1
CDS	69..314
Translation	MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLGQ

Target ORF information:

RefSeq Version	NM_008683.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008683.4

ORF Insert Sequence:

ATGCTAATTA AAGTGAAGAC GCTGACTGGG AAGGAGATTG AGATAGACAT CGAACCCACA 
GACAAGGTGG AGCGAATCAA GGAGCGTGTG GAAGAAAAAG AAGGGATTCC CCCCCAGCAG 
CAGCGGCTCA TCTACAGTGG CAAGCAAATG AATGATGAGA AGACAGCAGC TGATTACAAG 
ATTCTAGGTG GTTCCGTCCT CCACCTGGTG TTGGCTCTTA GAGGAGGAGG TGGTCTTGGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury.
American journal of physiology. Lung cellular and molecular physiology313(1)L104-L114(2017 Jul)
Zhu Z,Sun L,Hao R,Jiang H,Qian F,Ye RD

Promoting tumorigenesis in nasopharyngeal carcinoma, NEDD8 serves as a potential theranostic target.
Cell death & disease8(6)e2834(2017 Jun)
Xie P,Yang JP,Cao Y,Peng LX,Zheng LS,Sun R,Meng DF,Wang MY,Mei Y,Qiang YY,Cao L,Xiang YQ,Luo DH,Yun JP,Huang BJ,Jia LJ,Qian CN

An inhibitor of apoptosis protein antagonist T-3256336 potentiates the antitumor efficacy of the Nedd8-activating enzyme inhibitor pevonedistat (TAK-924/MLN4924).
Biochemical and biophysical research communications480(3)380-386(2016 Nov)
Sumi H,Inazuka M,Morimoto M,Hibino R,Hashimoto K,Ishikawa T,Kuida K,Smith PG,Yoshida S,Yabuki M

Identification of Novel Host Interactors of Effectors Secreted by
mSystems1(4)(2016 Jul-Aug)
Sontag RL,Nakayasu ES,Brown RN,Niemann GS,Sydor MA,Sanchez O,Ansong C,Lu SY,Choi H,Valleau D,Weitz KK,Savchenko A,Cambronne ED,Adkins JN

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Nedd8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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