Nedd4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nedd4
Entrez Gene ID	17999
Full Name	neural precursor cell expressed, developmentally down-regulated 4
Synonyms	AA959633,AL023035,AU019897,E430025J12Rik,EG639396,Gm7265,KIAA0093,Nedd4-1,Nedd4a
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated 4 Names E3 ubiquitin-protein ligase NEDD4 HECT-type E3 ubiquitin transferase NEDD4 neural precursor cell expressed developmentally down-regulated protein 4 neural precursor cell expressed, developmentally down-regulated gene 4a
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 D\|9 40.08 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010890.3	NP_035020.2	E3 ubiquitin-protein ligase NEDD4
NM_001357998.1	NP_001344927.1	E3 ubiquitin-protein ligase NEDD4 isoform 2
NM_010890.4	NP_035020.2	E3 ubiquitin-protein ligase NEDD4 isoform 1
XM_011242672.2	XP_011240974.1	E3 ubiquitin-protein ligase NEDD4 isoform X1
XM_011242672.3	XP_011240974.1	E3 ubiquitin-protein ligase NEDD4 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nedd4a	NP_001029358.1
Mus musculus (house mouse)	Nedd4	NP_035020.2
Schizosaccharomyces pombe (fission yeast)	pub3	NP_595793.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003133	XP_003436401.1
Pan troglodytes (chimpanzee)	NEDD4	XP_523083.3
Rattus norvegicus (Norway rat)	Nedd4	NP_037118.1

Gene Ontology
Bibliography

Related articles in PubMed

Large-Scale Quantitative Proteomics Identifies the Ubiquitin Ligase Nedd4-1 as an Essential Regulator of Liver Regeneration.
Bachofner M, Speicher T, Bogorad RL, Muzumdar S, Derrer CP, H?rlimann F, B?hm F, Nanni P, Kockmann T, Kachaylo E, Meyer M, Padrissa-Alt?s S, Graf R, Anderson DG, Koteliansky V, Auf dem Keller U, Werner S
Developmental cell42(6)616-625.e8(2017 09)

Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.
Li S, Mi L, Yu L, Yu Q, Liu T, Wang GX, Zhao XY, Wu J, Lin JD
Proceedings of the National Academy of Sciences of the United States of America114(34)E7111-E7120(2017 Aug)

The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy.
Pei G, Buijze H, Liu H, Moura-Alves P, Goosmann C, Brinkmann V, Kawabe H, Dorhoi A, Kaufmann SHE
Autophagy13(12)2041-2055(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nedd4-1 is required for efficient internalization of major growth factor receptors involved in liver regeneration and their downstream mitogenic signaling.
Title: Large-Scale Quantitative Proteomics Identifies the Ubiquitin Ligase Nedd4-1 as an Essential Regulator of Liver Regeneration.

NPM1-dependent nucleolar PIDDosome is a key initiator of the caspase-2 activation cascade.
Title: NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus.

Our data suggest that the behavioral sensitivity to stress is dramatically reduced in FXS, opening new views on the impact of gene-environment interactions in this pathology.
Title: Behavioral effects of chronic stress in the Fmr1 mouse model for fragile X syndrome.

these results suggest that Nedd4 suppresses colonic WNT signaling and tumor growth, at least in part, by suppressing the transcription factors LEF1 and YY1.
Title: Intestinal knockout of Nedd4 enhances growth of Apc

Upregulation of long non-coding RNA PAPAS in response to hypoosmotic stress does not increase H4K20me3 because of Nedd4-dependent ubiquitinylation and proteasomal degradation of Suv4-20h2.
Title: lncRNA-Induced Nucleosome Repositioning Reinforces Transcriptional Repression of rRNA Genes upon Hypotonic Stress.

The following Nedd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19109	NM_010890.4 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu68307	XM_011242672.3 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu83607	NM_001357998.1 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMu19109	NM_010890.3	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu68307	XM_011242672.2	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nedd4 ( NM_010890.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035020.2 Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), mRNA.

Price & Availability↑

CloneID	OMu19109
Clone ID Related Accession (Same CDS sequence)	NM_010890.3 , NM_010890.4
Accession Version	NM_010890.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-08
Organism	Mus musculus(House mouse)
Product	E3 ubiquitin-protein ligase NEDD4
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D85414.1, BY059586.1, AK122203.1, BY068934.1, BB542352.1, BQ899954.1, CD807110.1, BQ177728.1 and AW553531.2. On or before Dec 20, 2004 this sequence version replaced XM_486230.1, NM_010890.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTCGG ACATGGCAGC CGACGAGTCG GAGGCCCCAG TACTCTCGGA GGACGAGGTA 
TGGGAGTTTT GCCTGGATAA GACAGAAGAT GGTGGCGGAT CCCCCGGAAG TGATGTTACA 
GATACTTGTG AGCCTCCATG TGGATGCTGG GAGTTGAATC CGAATTCCCT GGAAGAGGAG 
CACGTGCTGT TCACTGCTGA TCCGTACCTG GAGCTCCACA ACGATGACAC ACGAGTTGTG 
AGAGTGAAGG TTATAGCTGG CATAGGCCTG GCCAAGAAAG ACATCTTGGG AGCCAGTGAT 
CCTTACGTAA GAGTGACATT GTATGACCCG ATGAGTGGAA TCCTTACCAG CGTGCAGACA 
AAAACTATCA AAAAGTCTTT GAATCCAAAA TGGAATGAAG AAATACTGTT CAGGGTCCTT 
CCACAGCGAC ACCGCATTCT TTTCGAAGTG TTTGATGAAA ATCGTTTGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTATTTA CCTAAAAATG GCTCAGAAGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACT 
CATTTGCCGC ATCCACCAGA ACCCTCTCCC CTACCTCCAG GATGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAATCTAGAA GAACACAGTG GAAAAGGCCA 
AGCCCTGACG ATGACCTCAC GGATGAAGAC AATGATGATA TGCAGCTGCA AGCGCAGCGA 
GCATTCACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCGGGAGTCC 
CCTGAGAATT GGGAAATCGT ACGAGAAGAT GAAAACACCG AGTATAGTGG TCAGGCTGTC 
CAGTCACCTC CATCGGGTCA CATTGATGTG CAGACTCACC TTGCAGAAGA GTTTAATACC 
AGACTTGCTG TGTGTGGAAA TCCAGCCACC AGCCAGCCGG TTACCAGCTC AAATCATTCC 
AGCAGAGGAG GCAGCTTGCA GACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGAAGA 
TCATACTATG TAGACCACAA CTCTAAAACC ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATCCC TGCTCATCTG AGAGGAAAGA CTGACTCCAA TGACCTGGGA 
CCCTTACCTC CAGGCTGGGA AGAAAGAACC CACACAGATG GGCGAGTCTT CTTCATAAAC 
CACAATATAA AGAAGACCCA GTGGGAAGAT CCTCGCCTGC AGAACGTGGC AATCACTGGA 
CCAGCAGTGC CCTACTCCAG AGATTACAAG AGAAAGTACG AGTTCTTCAG AAGGAAGCTC 
AAGAAGCAGA CTGACATTCC AAACAAATTT GAAATGAAGC TTCGCCGCGC AAACATTCTG 
GAGGATTCTT ACCGGAGGAT TATGGGTGTG AAGAGAGCTG ACTTGCTCAA GGCCAGACTC 
TGGATTGAGT TTGATGGTGA AAAGGGCCTT GACTATGGAG GGGTTGCCAG AGAGTGGTTC 
TTCCTCATCT CGAAGGAAAT GTTCAACCCT TACTACGGCC TGTTTGAATA TTCTGCTACG 
GATAATTACA CCCTACAGAT AAATCCTAAC TCGGGCTTGT GTAATGAAGA TCACCTCTCA 
TACTTCAAGT TCATTGGCCG TGTGGCTGGG ATGGCAGTTT ATCATGGCAA GCTGTTGGAT 
GGGTTTTTCA TCCGTCCGTT TTACAAGATG ATGCTTCAGA AACTGATAAC ACTGCACGAC 
ATGGAGTCCG TGGATAGTGA ATATTACAGT TCTCTGCGAT GGATTCTTGA AAATGACCCG 
ACGGAGCTGG ACCTGAGATT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAGCAC 
GAACTGAAAA CCGGAGGATC AGAGATTGTT GTCACCAATA AGAACAAAAA GGAGTATATC 
TACCTTGTAA TACAATGGCG ATTTGTGAAC CGTATCCAGA AGCAAATGGC AGCTTTTAAA 
GAGGGATTTT TTGAACTGAT ACCACAGGAT CTCATCAAGA TATTTGATGA AAATGAGCTA 
GAGCTTCTCA TGTGTGGTCT GGGAGATGTG GATGTGAACG ACTGGCGGGA ACACACAAAA 
TACAAAAATG GCTACAGCAT GAACCACCAG GTCATCCACT GGTTCTGGAA GGCTGTTTGG 
ATGATGGATT CGGAAAAAAG AATACGCTTA CTTCAGTTTG TCACTGGCAC ATCCCGTGTG 
CCGATGAATG GGTTTGCTGA ACTCTATGGC TCGAATGGAC CACAATCCTT CACAGTGGAA 
CAATGGGGCA CCCCTGATAA GCTGCCAAGA GCACACACCT GCTTCAATCG CCTGGACCTG 
CCACCCTACG AATCCTTTGA CGAACTCTGG GATAAACTTC AGATGGCAAT TGAGAACACA 
CAGGGCTTTG ATGGCGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035020.2
CDS	211..2874
Translation	MSSDMAADESEAPVLSEDEVWEFCLDKTEDGGGSPGSDVTDTCEPPCGCWELNPNSLEEEHVLFTADPYLELHNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSGILTSVQTKTIKKSLNPKWNEEILFRVLPQRHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSEDENADQAEELEPGWVVLDQPDAATHLPHPPEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRPSPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPDNRESPENWEIVREDENTEYSGQAVQSPPSGHIDVQTHLAEEFNTRLAVCGNPATSQPVTSSNHSSRGGSLQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_010890.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010890.3

ORF Insert Sequence:

ATGAGCTCGG ACATGGCAGC CGACGAGTCG GAGGCCCCAG TACTCTCGGA GGACGAGGTA 
TGGGAGTTTT GCCTGGATAA GACAGAAGAT GGTGGCGGAT CCCCCGGAAG TGATGTTACA 
GATACTTGTG AGCCTCCATG TGGATGCTGG GAGTTGAATC CGAATTCCCT GGAAGAGGAG 
CACGTGCTGT TCACTGCTGA TCCGTACCTG GAGCTCCACA ACGATGACAC ACGAGTTGTG 
AGAGTGAAGG TTATAGCTGG CATAGGCCTG GCCAAGAAAG ACATCTTGGG AGCCAGTGAT 
CCTTACGTAA GAGTGACATT GTATGACCCG ATGAGTGGAA TCCTTACCAG CGTGCAGACA 
AAAACTATCA AAAAGTCTTT GAATCCAAAA TGGAATGAAG AAATACTGTT CAGGGTCCTT 
CCACAGCGAC ACCGCATTCT TTTCGAAGTG TTTGATGAAA ATCGTTTGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTATTTA CCTAAAAATG GCTCAGAAGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACT 
CATTTGCCGC ATCCACCAGA ACCCTCTCCC CTACCTCCAG GATGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAATCTAGAA GAACACAGTG GAAAAGGCCA 
AGCCCTGACG ATGACCTCAC GGATGAAGAC AATGATGATA TGCAGCTGCA AGCGCAGCGA 
GCATTCACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCGGGAGTCC 
CCTGAGAATT GGGAAATCGT ACGAGAAGAT GAAAACACCG AGTATAGTGG TCAGGCTGTC 
CAGTCACCTC CATCGGGTCA CATTGATGTG CAGACTCACC TTGCAGAAGA GTTTAATACC 
AGACTTGCTG TGTGTGGAAA TCCAGCCACC AGCCAGCCGG TTACCAGCTC AAATCATTCC 
AGCAGAGGAG GCAGCTTGCA GACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGAAGA 
TCATACTATG TAGACCACAA CTCTAAAACC ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATCCC TGCTCATCTG AGAGGAAAGA CTGACTCCAA TGACCTGGGA 
CCCTTACCTC CAGGCTGGGA AGAAAGAACC CACACAGATG GGCGAGTCTT CTTCATAAAC 
CACAATATAA AGAAGACCCA GTGGGAAGAT CCTCGCCTGC AGAACGTGGC AATCACTGGA 
CCAGCAGTGC CCTACTCCAG AGATTACAAG AGAAAGTACG AGTTCTTCAG AAGGAAGCTC 
AAGAAGCAGA CTGACATTCC AAACAAATTT GAAATGAAGC TTCGCCGCGC AAACATTCTG 
GAGGATTCTT ACCGGAGGAT TATGGGTGTG AAGAGAGCTG ACTTGCTCAA GGCCAGACTC 
TGGATTGAGT TTGATGGTGA AAAGGGCCTT GACTATGGAG GGGTTGCCAG AGAGTGGTTC 
TTCCTCATCT CGAAGGAAAT GTTCAACCCT TACTACGGCC TGTTTGAATA TTCTGCTACG 
GATAATTACA CCCTACAGAT AAATCCTAAC TCGGGCTTGT GTAATGAAGA TCACCTCTCA 
TACTTCAAGT TCATTGGCCG TGTGGCTGGG ATGGCAGTTT ATCATGGCAA GCTGTTGGAT 
GGGTTTTTCA TCCGTCCGTT TTACAAGATG ATGCTTCAGA AACTGATAAC ACTGCACGAC 
ATGGAGTCCG TGGATAGTGA ATATTACAGT TCTCTGCGAT GGATTCTTGA AAATGACCCG 
ACGGAGCTGG ACCTGAGATT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAGCAC 
GAACTGAAAA CCGGAGGATC AGAGATTGTT GTCACCAATA AGAACAAAAA GGAGTATATC 
TACCTTGTAA TACAATGGCG ATTTGTGAAC CGTATCCAGA AGCAAATGGC AGCTTTTAAA 
GAGGGATTTT TTGAACTGAT ACCACAGGAT CTCATCAAGA TATTTGATGA AAATGAGCTA 
GAGCTTCTCA TGTGTGGTCT GGGAGATGTG GATGTGAACG ACTGGCGGGA ACACACAAAA 
TACAAAAATG GCTACAGCAT GAACCACCAG GTCATCCACT GGTTCTGGAA GGCTGTTTGG 
ATGATGGATT CGGAAAAAAG AATACGCTTA CTTCAGTTTG TCACTGGCAC ATCCCGTGTG 
CCGATGAATG GGTTTGCTGA ACTCTATGGC TCGAATGGAC CACAATCCTT CACAGTGGAA 
CAATGGGGCA CCCCTGATAA GCTGCCAAGA GCACACACCT GCTTCAATCG CCTGGACCTG 
CCACCCTACG AATCCTTTGA CGAACTCTGG GATAAACTTC AGATGGCAAT TGAGAACACA 
CAGGGCTTTG ATGGCGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd4 ( NM_010890.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035020.2 Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19109
Clone ID Related Accession (Same CDS sequence)	NM_010890.3 , NM_010890.4
Accession Version	NM_010890.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123683.6, AK133838.1, CF736340.1, CO426280.1, CX565415.1 and CX566785.1. On Nov 14, 2017 this sequence version replaced NM_010890.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D85414.1, U96635.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCGG ACATGGCAGC CGACGAGTCG GAGGCCCCAG TACTCTCGGA GGACGAGGTA 
TGGGAGTTTT GCCTGGATAA GACAGAAGAT GGTGGCGGAT CCCCCGGAAG TGATGTTACA 
GATACTTGTG AGCCTCCATG TGGATGCTGG GAGTTGAATC CGAATTCCCT GGAAGAGGAG 
CACGTGCTGT TCACTGCTGA TCCGTACCTG GAGCTCCACA ACGATGACAC ACGAGTTGTG 
AGAGTGAAGG TTATAGCTGG CATAGGCCTG GCCAAGAAAG ACATCTTGGG AGCCAGTGAT 
CCTTACGTAA GAGTGACATT GTATGACCCG ATGAGTGGAA TCCTTACCAG CGTGCAGACA 
AAAACTATCA AAAAGTCTTT GAATCCAAAA TGGAATGAAG AAATACTGTT CAGGGTCCTT 
CCACAGCGAC ACCGCATTCT TTTCGAAGTG TTTGATGAAA ATCGTTTGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTATTTA CCTAAAAATG GCTCAGAAGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACT 
CATTTGCCGC ATCCACCAGA ACCCTCTCCC CTACCTCCAG GATGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAATCTAGAA GAACACAGTG GAAAAGGCCA 
AGCCCTGACG ATGACCTCAC GGATGAAGAC AATGATGATA TGCAGCTGCA AGCGCAGCGA 
GCATTCACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCGGGAGTCC 
CCTGAGAATT GGGAAATCGT ACGAGAAGAT GAAAACACCG AGTATAGTGG TCAGGCTGTC 
CAGTCACCTC CATCGGGTCA CATTGATGTG CAGACTCACC TTGCAGAAGA GTTTAATACC 
AGACTTGCTG TGTGTGGAAA TCCAGCCACC AGCCAGCCGG TTACCAGCTC AAATCATTCC 
AGCAGAGGAG GCAGCTTGCA GACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGAAGA 
TCATACTATG TAGACCACAA CTCTAAAACC ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATCCC TGCTCATCTG AGAGGAAAGA CTGACTCCAA TGACCTGGGA 
CCCTTACCTC CAGGCTGGGA AGAAAGAACC CACACAGATG GGCGAGTCTT CTTCATAAAC 
CACAATATAA AGAAGACCCA GTGGGAAGAT CCTCGCCTGC AGAACGTGGC AATCACTGGA 
CCAGCAGTGC CCTACTCCAG AGATTACAAG AGAAAGTACG AGTTCTTCAG AAGGAAGCTC 
AAGAAGCAGA CTGACATTCC AAACAAATTT GAAATGAAGC TTCGCCGCGC AAACATTCTG 
GAGGATTCTT ACCGGAGGAT TATGGGTGTG AAGAGAGCTG ACTTGCTCAA GGCCAGACTC 
TGGATTGAGT TTGATGGTGA AAAGGGCCTT GACTATGGAG GGGTTGCCAG AGAGTGGTTC 
TTCCTCATCT CGAAGGAAAT GTTCAACCCT TACTACGGCC TGTTTGAATA TTCTGCTACG 
GATAATTACA CCCTACAGAT AAATCCTAAC TCGGGCTTGT GTAATGAAGA TCACCTCTCA 
TACTTCAAGT TCATTGGCCG TGTGGCTGGG ATGGCAGTTT ATCATGGCAA GCTGTTGGAT 
GGGTTTTTCA TCCGTCCGTT TTACAAGATG ATGCTTCAGA AACTGATAAC ACTGCACGAC 
ATGGAGTCCG TGGATAGTGA ATATTACAGT TCTCTGCGAT GGATTCTTGA AAATGACCCG 
ACGGAGCTGG ACCTGAGATT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAGCAC 
GAACTGAAAA CCGGAGGATC AGAGATTGTT GTCACCAATA AGAACAAAAA GGAGTATATC 
TACCTTGTAA TACAATGGCG ATTTGTGAAC CGTATCCAGA AGCAAATGGC AGCTTTTAAA 
GAGGGATTTT TTGAACTGAT ACCACAGGAT CTCATCAAGA TATTTGATGA AAATGAGCTA 
GAGCTTCTCA TGTGTGGTCT GGGAGATGTG GATGTGAACG ACTGGCGGGA ACACACAAAA 
TACAAAAATG GCTACAGCAT GAACCACCAG GTCATCCACT GGTTCTGGAA GGCTGTTTGG 
ATGATGGATT CGGAAAAAAG AATACGCTTA CTTCAGTTTG TCACTGGCAC ATCCCGTGTG 
CCGATGAATG GGTTTGCTGA ACTCTATGGC TCGAATGGAC CACAATCCTT CACAGTGGAA 
CAATGGGGCA CCCCTGATAA GCTGCCAAGA GCACACACCT GCTTCAATCG CCTGGACCTG 
CCACCCTACG AATCCTTTGA CGAACTCTGG GATAAACTTC AGATGGCAAT TGAGAACACA 
CAGGGCTTTG ATGGCGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035020.2
CDS	227..2890
Translation	MSSDMAADESEAPVLSEDEVWEFCLDKTEDGGGSPGSDVTDTCEPPCGCWELNPNSLEEEHVLFTADPYLELHNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSGILTSVQTKTIKKSLNPKWNEEILFRVLPQRHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSEDENADQAEELEPGWVVLDQPDAATHLPHPPEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRPSPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPDNRESPENWEIVREDENTEYSGQAVQSPPSGHIDVQTHLAEEFNTRLAVCGNPATSQPVTSSNHSSRGGSLQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_010890.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010890.4

ORF Insert Sequence:

ATGAGCTCGG ACATGGCAGC CGACGAGTCG GAGGCCCCAG TACTCTCGGA GGACGAGGTA 
TGGGAGTTTT GCCTGGATAA GACAGAAGAT GGTGGCGGAT CCCCCGGAAG TGATGTTACA 
GATACTTGTG AGCCTCCATG TGGATGCTGG GAGTTGAATC CGAATTCCCT GGAAGAGGAG 
CACGTGCTGT TCACTGCTGA TCCGTACCTG GAGCTCCACA ACGATGACAC ACGAGTTGTG 
AGAGTGAAGG TTATAGCTGG CATAGGCCTG GCCAAGAAAG ACATCTTGGG AGCCAGTGAT 
CCTTACGTAA GAGTGACATT GTATGACCCG ATGAGTGGAA TCCTTACCAG CGTGCAGACA 
AAAACTATCA AAAAGTCTTT GAATCCAAAA TGGAATGAAG AAATACTGTT CAGGGTCCTT 
CCACAGCGAC ACCGCATTCT TTTCGAAGTG TTTGATGAAA ATCGTTTGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTATTTA CCTAAAAATG GCTCAGAAGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACT 
CATTTGCCGC ATCCACCAGA ACCCTCTCCC CTACCTCCAG GATGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAATCTAGAA GAACACAGTG GAAAAGGCCA 
AGCCCTGACG ATGACCTCAC GGATGAAGAC AATGATGATA TGCAGCTGCA AGCGCAGCGA 
GCATTCACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCGGGAGTCC 
CCTGAGAATT GGGAAATCGT ACGAGAAGAT GAAAACACCG AGTATAGTGG TCAGGCTGTC 
CAGTCACCTC CATCGGGTCA CATTGATGTG CAGACTCACC TTGCAGAAGA GTTTAATACC 
AGACTTGCTG TGTGTGGAAA TCCAGCCACC AGCCAGCCGG TTACCAGCTC AAATCATTCC 
AGCAGAGGAG GCAGCTTGCA GACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGAAGA 
TCATACTATG TAGACCACAA CTCTAAAACC ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATCCC TGCTCATCTG AGAGGAAAGA CTGACTCCAA TGACCTGGGA 
CCCTTACCTC CAGGCTGGGA AGAAAGAACC CACACAGATG GGCGAGTCTT CTTCATAAAC 
CACAATATAA AGAAGACCCA GTGGGAAGAT CCTCGCCTGC AGAACGTGGC AATCACTGGA 
CCAGCAGTGC CCTACTCCAG AGATTACAAG AGAAAGTACG AGTTCTTCAG AAGGAAGCTC 
AAGAAGCAGA CTGACATTCC AAACAAATTT GAAATGAAGC TTCGCCGCGC AAACATTCTG 
GAGGATTCTT ACCGGAGGAT TATGGGTGTG AAGAGAGCTG ACTTGCTCAA GGCCAGACTC 
TGGATTGAGT TTGATGGTGA AAAGGGCCTT GACTATGGAG GGGTTGCCAG AGAGTGGTTC 
TTCCTCATCT CGAAGGAAAT GTTCAACCCT TACTACGGCC TGTTTGAATA TTCTGCTACG 
GATAATTACA CCCTACAGAT AAATCCTAAC TCGGGCTTGT GTAATGAAGA TCACCTCTCA 
TACTTCAAGT TCATTGGCCG TGTGGCTGGG ATGGCAGTTT ATCATGGCAA GCTGTTGGAT 
GGGTTTTTCA TCCGTCCGTT TTACAAGATG ATGCTTCAGA AACTGATAAC ACTGCACGAC 
ATGGAGTCCG TGGATAGTGA ATATTACAGT TCTCTGCGAT GGATTCTTGA AAATGACCCG 
ACGGAGCTGG ACCTGAGATT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAGCAC 
GAACTGAAAA CCGGAGGATC AGAGATTGTT GTCACCAATA AGAACAAAAA GGAGTATATC 
TACCTTGTAA TACAATGGCG ATTTGTGAAC CGTATCCAGA AGCAAATGGC AGCTTTTAAA 
GAGGGATTTT TTGAACTGAT ACCACAGGAT CTCATCAAGA TATTTGATGA AAATGAGCTA 
GAGCTTCTCA TGTGTGGTCT GGGAGATGTG GATGTGAACG ACTGGCGGGA ACACACAAAA 
TACAAAAATG GCTACAGCAT GAACCACCAG GTCATCCACT GGTTCTGGAA GGCTGTTTGG 
ATGATGGATT CGGAAAAAAG AATACGCTTA CTTCAGTTTG TCACTGGCAC ATCCCGTGTG 
CCGATGAATG GGTTTGCTGA ACTCTATGGC TCGAATGGAC CACAATCCTT CACAGTGGAA 
CAATGGGGCA CCCCTGATAA GCTGCCAAGA GCACACACCT GCTTCAATCG CCTGGACCTG 
CCACCCTACG AATCCTTTGA CGAACTCTGG GATAAACTTC AGATGGCAAT TGAGAACACA 
CAGGGCTTTG ATGGCGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd4 ( XM_011242672.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240974.1 Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant X1, mRNA.

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CloneID	OMu68307
Clone ID Related Accession (Same CDS sequence)	XM_011242672.2 , XM_011242672.3
Accession Version	XM_011242672.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAC CATATACATT TAAGGATTTT GTTCTTCATC CAAGAAGTCA CAAATCAAGA 
GTTAAAGGTT ATCTGAGATT AAAAATGACT TATTTACCTA AAAATGGCTC AGAAGATGAA 
AATGCAGACC AGGCTGAGGA GTTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCCACTCATT TGCCGCATCC ACCAGAACCC TCTCCCCTAC CTCCAGGATG GGAAGAGAGG 
CAGGATGTCC TTGGAAGGAC CTACTACGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGGCCAAGCC CTGACGATGA CCTCACGGAT GAAGACAATG ATGATATGCA GCTGCAAGCG 
CAGCGAGCAT TCACCACCAG GCGGCAGATA TCGGAGGATG TGGATGGCCC TGACAACCGG 
GAGTCCCCTG AGAATTGGGA AATCGTACGA GAAGATGAAA ACACCGAGTA TAGTGGTCAG 
GCTGTCCAGT CACCTCCATC GGGTCACATT GATGTGCAGA CTCACCTTGC AGAAGAGTTT 
AATACCAGAC TTGCTGTGTG TGGAAATCCA GCCACCAGCC AGCCGGTTAC CAGCTCAAAT 
CATTCCAGCA GAGGAGGCAG CTTGCAGACC TGTATCTTTG AGGAACAGCC TACACTTCCT 
GTGCTTTTGC CTACTTCATC TGGATTGCCA CCAGGTTGGG AAGAAAAACA AGATGACAGA 
GGAAGATCAT ACTATGTAGA CCACAACTCT AAAACCACCA CATGGTCCAA GCCCACCATG 
CAGGATGATC CAAGATCGAA AATCCCTGCT CATCTGAGAG GAAAGACTGA CTCCAATGAC 
CTGGGACCCT TACCTCCAGG CTGGGAAGAA AGAACCCACA CAGATGGGCG AGTCTTCTTC 
ATAAACCACA ATATAAAGAA GACCCAGTGG GAAGATCCTC GCCTGCAGAA CGTGGCAATC 
ACTGGACCAG CAGTGCCCTA CTCCAGAGAT TACAAGAGAA AGTACGAGTT CTTCAGAAGG 
AAGCTCAAGA AGCAGACTGA CATTCCAAAC AAATTTGAAA TGAAGCTTCG CCGCGCAAAC 
ATTCTGGAGG ATTCTTACCG GAGGATTATG GGTGTGAAGA GAGCTGACTT GCTCAAGGCC 
AGACTCTGGA TTGAGTTTGA TGGTGAAAAG GGCCTTGACT ATGGAGGGGT TGCCAGAGAG 
TGGTTCTTCC TCATCTCGAA GGAAATGTTC AACCCTTACT ACGGCCTGTT TGAATATTCT 
GCTACGGATA ATTACACCCT ACAGATAAAT CCTAACTCGG GCTTGTGTAA TGAAGATCAC 
CTCTCATACT TCAAGTTCAT TGGCCGTGTG GCTGGGATGG CAGTTTATCA TGGCAAGCTG 
TTGGATGGGT TTTTCATCCG TCCGTTTTAC AAGATGATGC TTCAGAAACT GATAACACTG 
CACGACATGG AGTCCGTGGA TAGTGAATAT TACAGTTCTC TGCGATGGAT TCTTGAAAAT 
GACCCGACGG AGCTGGACCT GAGATTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAGCACGAAC TGAAAACCGG AGGATCAGAG ATTGTTGTCA CCAATAAGAA CAAAAAGGAG 
TATATCTACC TTGTAATACA ATGGCGATTT GTGAACCGTA TCCAGAAGCA AATGGCAGCT 
TTTAAAGAGG GATTTTTTGA ACTGATACCA CAGGATCTCA TCAAGATATT TGATGAAAAT 
GAGCTAGAGC TTCTCATGTG TGGTCTGGGA GATGTGGATG TGAACGACTG GCGGGAACAC 
ACAAAATACA AAAATGGCTA CAGCATGAAC CACCAGGTCA TCCACTGGTT CTGGAAGGCT 
GTTTGGATGA TGGATTCGGA AAAAAGAATA CGCTTACTTC AGTTTGTCAC TGGCACATCC 
CGTGTGCCGA TGAATGGGTT TGCTGAACTC TATGGCTCGA ATGGACCACA ATCCTTCACA 
GTGGAACAAT GGGGCACCCC TGATAAGCTG CCAAGAGCAC ACACCTGCTT CAATCGCCTG 
GACCTGCCAC CCTACGAATC CTTTGACGAA CTCTGGGATA AACTTCAGAT GGCAATTGAG 
AACACACAGG GCTTTGATGG CGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240974.1
CDS	75..2204
Translation	MERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSEDENADQAEELEPGWVVLDQPDAATHLPHPPEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRPSPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPDNRESPENWEIVREDENTEYSGQAVQSPPSGHIDVQTHLAEEFNTRLAVCGNPATSQPVTSSNHSSRGGSLQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011242672.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242672.2

ORF Insert Sequence:

ATGGAGAGAC CATATACATT TAAGGATTTT GTTCTTCATC CAAGAAGTCA CAAATCAAGA 
GTTAAAGGTT ATCTGAGATT AAAAATGACT TATTTACCTA AAAATGGCTC AGAAGATGAA 
AATGCAGACC AGGCTGAGGA GTTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCCACTCATT TGCCGCATCC ACCAGAACCC TCTCCCCTAC CTCCAGGATG GGAAGAGAGG 
CAGGATGTCC TTGGAAGGAC CTACTACGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGGCCAAGCC CTGACGATGA CCTCACGGAT GAAGACAATG ATGATATGCA GCTGCAAGCG 
CAGCGAGCAT TCACCACCAG GCGGCAGATA TCGGAGGATG TGGATGGCCC TGACAACCGG 
GAGTCCCCTG AGAATTGGGA AATCGTACGA GAAGATGAAA ACACCGAGTA TAGTGGTCAG 
GCTGTCCAGT CACCTCCATC GGGTCACATT GATGTGCAGA CTCACCTTGC AGAAGAGTTT 
AATACCAGAC TTGCTGTGTG TGGAAATCCA GCCACCAGCC AGCCGGTTAC CAGCTCAAAT 
CATTCCAGCA GAGGAGGCAG CTTGCAGACC TGTATCTTTG AGGAACAGCC TACACTTCCT 
GTGCTTTTGC CTACTTCATC TGGATTGCCA CCAGGTTGGG AAGAAAAACA AGATGACAGA 
GGAAGATCAT ACTATGTAGA CCACAACTCT AAAACCACCA CATGGTCCAA GCCCACCATG 
CAGGATGATC CAAGATCGAA AATCCCTGCT CATCTGAGAG GAAAGACTGA CTCCAATGAC 
CTGGGACCCT TACCTCCAGG CTGGGAAGAA AGAACCCACA CAGATGGGCG AGTCTTCTTC 
ATAAACCACA ATATAAAGAA GACCCAGTGG GAAGATCCTC GCCTGCAGAA CGTGGCAATC 
ACTGGACCAG CAGTGCCCTA CTCCAGAGAT TACAAGAGAA AGTACGAGTT CTTCAGAAGG 
AAGCTCAAGA AGCAGACTGA CATTCCAAAC AAATTTGAAA TGAAGCTTCG CCGCGCAAAC 
ATTCTGGAGG ATTCTTACCG GAGGATTATG GGTGTGAAGA GAGCTGACTT GCTCAAGGCC 
AGACTCTGGA TTGAGTTTGA TGGTGAAAAG GGCCTTGACT ATGGAGGGGT TGCCAGAGAG 
TGGTTCTTCC TCATCTCGAA GGAAATGTTC AACCCTTACT ACGGCCTGTT TGAATATTCT 
GCTACGGATA ATTACACCCT ACAGATAAAT CCTAACTCGG GCTTGTGTAA TGAAGATCAC 
CTCTCATACT TCAAGTTCAT TGGCCGTGTG GCTGGGATGG CAGTTTATCA TGGCAAGCTG 
TTGGATGGGT TTTTCATCCG TCCGTTTTAC AAGATGATGC TTCAGAAACT GATAACACTG 
CACGACATGG AGTCCGTGGA TAGTGAATAT TACAGTTCTC TGCGATGGAT TCTTGAAAAT 
GACCCGACGG AGCTGGACCT GAGATTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAGCACGAAC TGAAAACCGG AGGATCAGAG ATTGTTGTCA CCAATAAGAA CAAAAAGGAG 
TATATCTACC TTGTAATACA ATGGCGATTT GTGAACCGTA TCCAGAAGCA AATGGCAGCT 
TTTAAAGAGG GATTTTTTGA ACTGATACCA CAGGATCTCA TCAAGATATT TGATGAAAAT 
GAGCTAGAGC TTCTCATGTG TGGTCTGGGA GATGTGGATG TGAACGACTG GCGGGAACAC 
ACAAAATACA AAAATGGCTA CAGCATGAAC CACCAGGTCA TCCACTGGTT CTGGAAGGCT 
GTTTGGATGA TGGATTCGGA AAAAAGAATA CGCTTACTTC AGTTTGTCAC TGGCACATCC 
CGTGTGCCGA TGAATGGGTT TGCTGAACTC TATGGCTCGA ATGGACCACA ATCCTTCACA 
GTGGAACAAT GGGGCACCCC TGATAAGCTG CCAAGAGCAC ACACCTGCTT CAATCGCCTG 
GACCTGCCAC CCTACGAATC CTTTGACGAA CTCTGGGATA AACTTCAGAT GGCAATTGAG 
AACACACAGG GCTTTGATGG CGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd4 ( XM_011242672.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240974.1 Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68307
Clone ID Related Accession (Same CDS sequence)	XM_011242672.2 , XM_011242672.3
Accession Version	XM_011242672.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAC CATATACATT TAAGGATTTT GTTCTTCATC CAAGAAGTCA CAAATCAAGA 
GTTAAAGGTT ATCTGAGATT AAAAATGACT TATTTACCTA AAAATGGCTC AGAAGATGAA 
AATGCAGACC AGGCTGAGGA GTTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCCACTCATT TGCCGCATCC ACCAGAACCC TCTCCCCTAC CTCCAGGATG GGAAGAGAGG 
CAGGATGTCC TTGGAAGGAC CTACTACGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGGCCAAGCC CTGACGATGA CCTCACGGAT GAAGACAATG ATGATATGCA GCTGCAAGCG 
CAGCGAGCAT TCACCACCAG GCGGCAGATA TCGGAGGATG TGGATGGCCC TGACAACCGG 
GAGTCCCCTG AGAATTGGGA AATCGTACGA GAAGATGAAA ACACCGAGTA TAGTGGTCAG 
GCTGTCCAGT CACCTCCATC GGGTCACATT GATGTGCAGA CTCACCTTGC AGAAGAGTTT 
AATACCAGAC TTGCTGTGTG TGGAAATCCA GCCACCAGCC AGCCGGTTAC CAGCTCAAAT 
CATTCCAGCA GAGGAGGCAG CTTGCAGACC TGTATCTTTG AGGAACAGCC TACACTTCCT 
GTGCTTTTGC CTACTTCATC TGGATTGCCA CCAGGTTGGG AAGAAAAACA AGATGACAGA 
GGAAGATCAT ACTATGTAGA CCACAACTCT AAAACCACCA CATGGTCCAA GCCCACCATG 
CAGGATGATC CAAGATCGAA AATCCCTGCT CATCTGAGAG GAAAGACTGA CTCCAATGAC 
CTGGGACCCT TACCTCCAGG CTGGGAAGAA AGAACCCACA CAGATGGGCG AGTCTTCTTC 
ATAAACCACA ATATAAAGAA GACCCAGTGG GAAGATCCTC GCCTGCAGAA CGTGGCAATC 
ACTGGACCAG CAGTGCCCTA CTCCAGAGAT TACAAGAGAA AGTACGAGTT CTTCAGAAGG 
AAGCTCAAGA AGCAGACTGA CATTCCAAAC AAATTTGAAA TGAAGCTTCG CCGCGCAAAC 
ATTCTGGAGG ATTCTTACCG GAGGATTATG GGTGTGAAGA GAGCTGACTT GCTCAAGGCC 
AGACTCTGGA TTGAGTTTGA TGGTGAAAAG GGCCTTGACT ATGGAGGGGT TGCCAGAGAG 
TGGTTCTTCC TCATCTCGAA GGAAATGTTC AACCCTTACT ACGGCCTGTT TGAATATTCT 
GCTACGGATA ATTACACCCT ACAGATAAAT CCTAACTCGG GCTTGTGTAA TGAAGATCAC 
CTCTCATACT TCAAGTTCAT TGGCCGTGTG GCTGGGATGG CAGTTTATCA TGGCAAGCTG 
TTGGATGGGT TTTTCATCCG TCCGTTTTAC AAGATGATGC TTCAGAAACT GATAACACTG 
CACGACATGG AGTCCGTGGA TAGTGAATAT TACAGTTCTC TGCGATGGAT TCTTGAAAAT 
GACCCGACGG AGCTGGACCT GAGATTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAGCACGAAC TGAAAACCGG AGGATCAGAG ATTGTTGTCA CCAATAAGAA CAAAAAGGAG 
TATATCTACC TTGTAATACA ATGGCGATTT GTGAACCGTA TCCAGAAGCA AATGGCAGCT 
TTTAAAGAGG GATTTTTTGA ACTGATACCA CAGGATCTCA TCAAGATATT TGATGAAAAT 
GAGCTAGAGC TTCTCATGTG TGGTCTGGGA GATGTGGATG TGAACGACTG GCGGGAACAC 
ACAAAATACA AAAATGGCTA CAGCATGAAC CACCAGGTCA TCCACTGGTT CTGGAAGGCT 
GTTTGGATGA TGGATTCGGA AAAAAGAATA CGCTTACTTC AGTTTGTCAC TGGCACATCC 
CGTGTGCCGA TGAATGGGTT TGCTGAACTC TATGGCTCGA ATGGACCACA ATCCTTCACA 
GTGGAACAAT GGGGCACCCC TGATAAGCTG CCAAGAGCAC ACACCTGCTT CAATCGCCTG 
GACCTGCCAC CCTACGAATC CTTTGACGAA CTCTGGGATA AACTTCAGAT GGCAATTGAG 
AACACACAGG GCTTTGATGG CGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240974.1
CDS	75..2204
Translation	MERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSEDENADQAEELEPGWVVLDQPDAATHLPHPPEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRPSPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPDNRESPENWEIVREDENTEYSGQAVQSPPSGHIDVQTHLAEEFNTRLAVCGNPATSQPVTSSNHSSRGGSLQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011242672.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242672.3

ORF Insert Sequence:

ATGGAGAGAC CATATACATT TAAGGATTTT GTTCTTCATC CAAGAAGTCA CAAATCAAGA 
GTTAAAGGTT ATCTGAGATT AAAAATGACT TATTTACCTA AAAATGGCTC AGAAGATGAA 
AATGCAGACC AGGCTGAGGA GTTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCCACTCATT TGCCGCATCC ACCAGAACCC TCTCCCCTAC CTCCAGGATG GGAAGAGAGG 
CAGGATGTCC TTGGAAGGAC CTACTACGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGGCCAAGCC CTGACGATGA CCTCACGGAT GAAGACAATG ATGATATGCA GCTGCAAGCG 
CAGCGAGCAT TCACCACCAG GCGGCAGATA TCGGAGGATG TGGATGGCCC TGACAACCGG 
GAGTCCCCTG AGAATTGGGA AATCGTACGA GAAGATGAAA ACACCGAGTA TAGTGGTCAG 
GCTGTCCAGT CACCTCCATC GGGTCACATT GATGTGCAGA CTCACCTTGC AGAAGAGTTT 
AATACCAGAC TTGCTGTGTG TGGAAATCCA GCCACCAGCC AGCCGGTTAC CAGCTCAAAT 
CATTCCAGCA GAGGAGGCAG CTTGCAGACC TGTATCTTTG AGGAACAGCC TACACTTCCT 
GTGCTTTTGC CTACTTCATC TGGATTGCCA CCAGGTTGGG AAGAAAAACA AGATGACAGA 
GGAAGATCAT ACTATGTAGA CCACAACTCT AAAACCACCA CATGGTCCAA GCCCACCATG 
CAGGATGATC CAAGATCGAA AATCCCTGCT CATCTGAGAG GAAAGACTGA CTCCAATGAC 
CTGGGACCCT TACCTCCAGG CTGGGAAGAA AGAACCCACA CAGATGGGCG AGTCTTCTTC 
ATAAACCACA ATATAAAGAA GACCCAGTGG GAAGATCCTC GCCTGCAGAA CGTGGCAATC 
ACTGGACCAG CAGTGCCCTA CTCCAGAGAT TACAAGAGAA AGTACGAGTT CTTCAGAAGG 
AAGCTCAAGA AGCAGACTGA CATTCCAAAC AAATTTGAAA TGAAGCTTCG CCGCGCAAAC 
ATTCTGGAGG ATTCTTACCG GAGGATTATG GGTGTGAAGA GAGCTGACTT GCTCAAGGCC 
AGACTCTGGA TTGAGTTTGA TGGTGAAAAG GGCCTTGACT ATGGAGGGGT TGCCAGAGAG 
TGGTTCTTCC TCATCTCGAA GGAAATGTTC AACCCTTACT ACGGCCTGTT TGAATATTCT 
GCTACGGATA ATTACACCCT ACAGATAAAT CCTAACTCGG GCTTGTGTAA TGAAGATCAC 
CTCTCATACT TCAAGTTCAT TGGCCGTGTG GCTGGGATGG CAGTTTATCA TGGCAAGCTG 
TTGGATGGGT TTTTCATCCG TCCGTTTTAC AAGATGATGC TTCAGAAACT GATAACACTG 
CACGACATGG AGTCCGTGGA TAGTGAATAT TACAGTTCTC TGCGATGGAT TCTTGAAAAT 
GACCCGACGG AGCTGGACCT GAGATTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAGCACGAAC TGAAAACCGG AGGATCAGAG ATTGTTGTCA CCAATAAGAA CAAAAAGGAG 
TATATCTACC TTGTAATACA ATGGCGATTT GTGAACCGTA TCCAGAAGCA AATGGCAGCT 
TTTAAAGAGG GATTTTTTGA ACTGATACCA CAGGATCTCA TCAAGATATT TGATGAAAAT 
GAGCTAGAGC TTCTCATGTG TGGTCTGGGA GATGTGGATG TGAACGACTG GCGGGAACAC 
ACAAAATACA AAAATGGCTA CAGCATGAAC CACCAGGTCA TCCACTGGTT CTGGAAGGCT 
GTTTGGATGA TGGATTCGGA AAAAAGAATA CGCTTACTTC AGTTTGTCAC TGGCACATCC 
CGTGTGCCGA TGAATGGGTT TGCTGAACTC TATGGCTCGA ATGGACCACA ATCCTTCACA 
GTGGAACAAT GGGGCACCCC TGATAAGCTG CCAAGAGCAC ACACCTGCTT CAATCGCCTG 
GACCTGCCAC CCTACGAATC CTTTGACGAA CTCTGGGATA AACTTCAGAT GGCAATTGAG 
AACACACAGG GCTTTGATGG CGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd4 ( NM_001357998.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344927.1 Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant 2, mRNA.

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CloneID	OMu83607
Clone ID Related Accession (Same CDS sequence)	NM_001357998.1
Accession Version	NM_001357998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-20
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123683.6 and CT027588.10. Transcript Variant: This variant (2) lacks multiple 5' exons and contains an alternate 5' UTR and 5' coding region compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.268637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACAGA GTTTACGACT GCACTTTGCC GCCAGAAGAA GCAAGACTTA CCCTCTGTCG 
GAACCCTCTG GAGATGACTT GGATAGCCAC GTTGACACAC ACGGCCAGAG GCGGCCCCGG 
ATCTCAGTGG CTAGTGTGGT TCAAATGAGG CTGACCCCCA GACAGGCGCC ACAGGCTCCG 
TGGGTAGAGC ATAGATCCTC TGAAAGTACT GACAAAAAGA GCAGCTGCCT GCAGATCTCA 
CTGCAGCCCA GATACGGAGG GTGTCTTCCA TCTGACACTG TCGTGGCTGA TAGGGACAGT 
ACTTCCTCTA CCTGTGCTTC AAAGGATGAT GTTTGCAGCA GCAGTGGAAT TGACAGTGAG 
CTGGACAATG GCAGCCTCAG TAACTTCTCA GCCAGTGACA GTGGGTCTTA CAGCAGCAGT 
GGCAGTGACT GTGGGTCTTG TGCAAGCCTC GCAAGTGGAG GCTCCTCCAC CGGCAGTGGT 
GTCAGCGACA GTAGTGGCTA TGAGACCTTT TTCAGTGGAA ATTTACCTTC TGACAACGCC 
TCCAACAGAA GCGTGCCAGT CGACACTACA CCCCGTGAAG TTATGTGCGG AAGTCCCAGT 
AGACTTCCCT TTGCCCAGGA GGACCTGGAT CGTGGAGTAG AGATTATGAA ACTGCCAGTG 
AGTAGGAACG CAAAGGGTCC ACTGAAGCGC TGCACGTCTT TGGTCATTTT CCCCAGGAGT 
CCTTCAAACA CCCCACCAGC ATCCCCGACG AGCAGAGGTG CCTACCAGAC CTCACACCAG 
TTGGTTGTAT CACCTACTGA AACTGCACAT CATGAAGATG GATCTGGCAC CAAAGGCTTT 
CTGTCAACAG CTGTCAACGG GCTTCGATTA TCTAAAATGG TTTGTACTCC TGGAGAAGTC 
AGAGATGTAC GGCCACTCCA TGCCAAGGGC TCATTGCAAA AGAAAATTGT CATTTCAAAT 
AATAGGCCCA AACAAACTGT GTGTGAGAAG TTGCCAGAAG GATTTTCCTG TGTTTCCGTG 
CACTTGACCC AACAAAAACC AACGACATCA GATTGTGCAA ACCCCCGTGG TGGCTGCACA 
TCAGAGAGGT TAGAACTTCA GCTGCAGTCT GATTTGGACC ATGGCATGCT CAAACAGAGG 
GCATCCCCTT GCTTGCCAAC CGCATCAGAC ATGGGTTACC ATTTAAACAT CCGTGGAGAG 
TTGGAAAGAC CAAATTCACC GATAAGCAAA GGCCATGCTG CTTTGCAAAG AAGTGTTTCG 
CTGGGTGGGA CATACCCAAA TATTTCCTGC TTATCCAGCC TGAAGCACAA TTGTTCAAAG 
GGGGGACCAT CTCAGTTGCT CATTAAGTTT GCATCTGGGA ATGATGGTAA AGTTGATAGT 
TTCTGGAGAC AGAGAGACTT GACAAATGAA CTGTCTGATG TTTGTAAGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTATTTA CCTAAAAATG GCTCAGAAGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACT 
CATTTGCCGC ATCCACCAGA ACCCTCTCCC CTACCTCCAG GATGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAATCTAGAA GAACACAGTG GAAAAGGCCA 
AGCCCTGACG ATGACCTCAC GGATGAAGAC AATGATGATA TGCAGCTGCA AGCGCAGCGA 
GCATTCACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCGGGAGTCC 
CCTGAGAATT GGGAAATCGT ACGAGAAGAT GAAAACACCG AGTATAGTGG TCAGGCTGTC 
CAGTCACCTC CATCGGGTCA CATTGATGTG CAGACTCACC TTGCAGAAGA GTTTAATACC 
AGACTTGCTG TGTGTGGAAA TCCAGCCACC AGCCAGCCGG TTACCAGCTC AAATCATTCC 
AGCAGAGGAG GCAGCTTGCA GACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGAAGA 
TCATACTATG TAGACCACAA CTCTAAAACC ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATCCC TGCTCATCTG AGAGGAAAGA CTGACTCCAA TGACCTGGGA 
CCCTTACCTC CAGGCTGGGA AGAAAGAACC CACACAGATG GGCGAGTCTT CTTCATAAAC 
CACAATATAA AGAAGACCCA GTGGGAAGAT CCTCGCCTGC AGAACGTGGC AATCACTGGA 
CCAGCAGTGC CCTACTCCAG AGATTACAAG AGAAAGTACG AGTTCTTCAG AAGGAAGCTC 
AAGAAGCAGA CTGACATTCC AAACAAATTT GAAATGAAGC TTCGCCGCGC AAACATTCTG 
GAGGATTCTT ACCGGAGGAT TATGGGTGTG AAGAGAGCTG ACTTGCTCAA GGCCAGACTC 
TGGATTGAGT TTGATGGTGA AAAGGGCCTT GACTATGGAG GGGTTGCCAG AGAGTGGTTC 
TTCCTCATCT CGAAGGAAAT GTTCAACCCT TACTACGGCC TGTTTGAATA TTCTGCTACG 
GATAATTACA CCCTACAGAT AAATCCTAAC TCGGGCTTGT GTAATGAAGA TCACCTCTCA 
TACTTCAAGT TCATTGGCCG TGTGGCTGGG ATGGCAGTTT ATCATGGCAA GCTGTTGGAT 
GGGTTTTTCA TCCGTCCGTT TTACAAGATG ATGCTTCAGA AACTGATAAC ACTGCACGAC 
ATGGAGTCCG TGGATAGTGA ATATTACAGT TCTCTGCGAT GGATTCTTGA AAATGACCCG 
ACGGAGCTGG ACCTGAGATT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAGCAC 
GAACTGAAAA CCGGAGGATC AGAGATTGTT GTCACCAATA AGAACAAAAA GGAGTATATC 
TACCTTGTAA TACAATGGCG ATTTGTGAAC CGTATCCAGA AGCAAATGGC AGCTTTTAAA 
GAGGGATTTT TTGAACTGAT ACCACAGGAT CTCATCAAGA TATTTGATGA AAATGAGCTA 
GAGCTTCTCA TGTGTGGTCT GGGAGATGTG GATGTGAACG ACTGGCGGGA ACACACAAAA 
TACAAAAATG GCTACAGCAT GAACCACCAG GTCATCCACT GGTTCTGGAA GGCTGTTTGG 
ATGATGGATT CGGAAAAAAG AATACGCTTA CTTCAGTTTG TCACTGGCAC ATCCCGTGTG 
CCGATGAATG GGTTTGCTGA ACTCTATGGC TCGAATGGAC CACAATCCTT CACAGTGGAA 
CAATGGGGCA CCCCTGATAA GCTGCCAAGA GCACACACCT GCTTCAATCG CCTGGACCTG 
CCACCCTACG AATCCTTTGA CGAACTCTGG GATAAACTTC AGATGGCAAT TGAGAACACA 
CAGGGCTTTG ATGGCGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344927.1
CDS	142..3765
Translation	MAQSLRLHFAARRSKTYPLSEPSGDDLDSHVDTHGQRRPRISVASVVQMRLTPRQAPQAPWVEHRSSESTDKKSSCLQISLQPRYGGCLPSDTVVADRDSTSSTCASKDDVCSSSGIDSELDNGSLSNFSASDSGSYSSSGSDCGSCASLASGGSSTGSGVSDSSGYETFFSGNLPSDNASNRSVPVDTTPREVMCGSPSRLPFAQEDLDRGVEIMKLPVSRNAKGPLKRCTSLVIFPRSPSNTPPASPTSRGAYQTSHQLVVSPTETAHHEDGSGTKGFLSTAVNGLRLSKMVCTPGEVRDVRPLHAKGSLQKKIVISNNRPKQTVCEKLPEGFSCVSVHLTQQKPTTSDCANPRGGCTSERLELQLQSDLDHGMLKQRASPCLPTASDMGYHLNIRGELERPNSPISKGHAALQRSVSLGGTYPNISCLSSLKHNCSKGGPSQLLIKFASGNDGKVDSFWRQRDLTNELSDVCKTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSEDENADQAEELEPGWVVLDQPDAATHLPHPPEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRPSPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPDNRESPENWEIVREDENTEYSGQAVQSPPSGHIDVQTHLAEEFNTRLAVCGNPATSQPVTSSNHSSRGGSLQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_001357998.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357998.1

ORF Insert Sequence:

ATGGCACAGA GTTTACGACT GCACTTTGCC GCCAGAAGAA GCAAGACTTA CCCTCTGTCG 
GAACCCTCTG GAGATGACTT GGATAGCCAC GTTGACACAC ACGGCCAGAG GCGGCCCCGG 
ATCTCAGTGG CTAGTGTGGT TCAAATGAGG CTGACCCCCA GACAGGCGCC ACAGGCTCCG 
TGGGTAGAGC ATAGATCCTC TGAAAGTACT GACAAAAAGA GCAGCTGCCT GCAGATCTCA 
CTGCAGCCCA GATACGGAGG GTGTCTTCCA TCTGACACTG TCGTGGCTGA TAGGGACAGT 
ACTTCCTCTA CCTGTGCTTC AAAGGATGAT GTTTGCAGCA GCAGTGGAAT TGACAGTGAG 
CTGGACAATG GCAGCCTCAG TAACTTCTCA GCCAGTGACA GTGGGTCTTA CAGCAGCAGT 
GGCAGTGACT GTGGGTCTTG TGCAAGCCTC GCAAGTGGAG GCTCCTCCAC CGGCAGTGGT 
GTCAGCGACA GTAGTGGCTA TGAGACCTTT TTCAGTGGAA ATTTACCTTC TGACAACGCC 
TCCAACAGAA GCGTGCCAGT CGACACTACA CCCCGTGAAG TTATGTGCGG AAGTCCCAGT 
AGACTTCCCT TTGCCCAGGA GGACCTGGAT CGTGGAGTAG AGATTATGAA ACTGCCAGTG 
AGTAGGAACG CAAAGGGTCC ACTGAAGCGC TGCACGTCTT TGGTCATTTT CCCCAGGAGT 
CCTTCAAACA CCCCACCAGC ATCCCCGACG AGCAGAGGTG CCTACCAGAC CTCACACCAG 
TTGGTTGTAT CACCTACTGA AACTGCACAT CATGAAGATG GATCTGGCAC CAAAGGCTTT 
CTGTCAACAG CTGTCAACGG GCTTCGATTA TCTAAAATGG TTTGTACTCC TGGAGAAGTC 
AGAGATGTAC GGCCACTCCA TGCCAAGGGC TCATTGCAAA AGAAAATTGT CATTTCAAAT 
AATAGGCCCA AACAAACTGT GTGTGAGAAG TTGCCAGAAG GATTTTCCTG TGTTTCCGTG 
CACTTGACCC AACAAAAACC AACGACATCA GATTGTGCAA ACCCCCGTGG TGGCTGCACA 
TCAGAGAGGT TAGAACTTCA GCTGCAGTCT GATTTGGACC ATGGCATGCT CAAACAGAGG 
GCATCCCCTT GCTTGCCAAC CGCATCAGAC ATGGGTTACC ATTTAAACAT CCGTGGAGAG 
TTGGAAAGAC CAAATTCACC GATAAGCAAA GGCCATGCTG CTTTGCAAAG AAGTGTTTCG 
CTGGGTGGGA CATACCCAAA TATTTCCTGC TTATCCAGCC TGAAGCACAA TTGTTCAAAG 
GGGGGACCAT CTCAGTTGCT CATTAAGTTT GCATCTGGGA ATGATGGTAA AGTTGATAGT 
TTCTGGAGAC AGAGAGACTT GACAAATGAA CTGTCTGATG TTTGTAAGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTATTTA CCTAAAAATG GCTCAGAAGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACT 
CATTTGCCGC ATCCACCAGA ACCCTCTCCC CTACCTCCAG GATGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAATCTAGAA GAACACAGTG GAAAAGGCCA 
AGCCCTGACG ATGACCTCAC GGATGAAGAC AATGATGATA TGCAGCTGCA AGCGCAGCGA 
GCATTCACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCGGGAGTCC 
CCTGAGAATT GGGAAATCGT ACGAGAAGAT GAAAACACCG AGTATAGTGG TCAGGCTGTC 
CAGTCACCTC CATCGGGTCA CATTGATGTG CAGACTCACC TTGCAGAAGA GTTTAATACC 
AGACTTGCTG TGTGTGGAAA TCCAGCCACC AGCCAGCCGG TTACCAGCTC AAATCATTCC 
AGCAGAGGAG GCAGCTTGCA GACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGAAGA 
TCATACTATG TAGACCACAA CTCTAAAACC ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATCCC TGCTCATCTG AGAGGAAAGA CTGACTCCAA TGACCTGGGA 
CCCTTACCTC CAGGCTGGGA AGAAAGAACC CACACAGATG GGCGAGTCTT CTTCATAAAC 
CACAATATAA AGAAGACCCA GTGGGAAGAT CCTCGCCTGC AGAACGTGGC AATCACTGGA 
CCAGCAGTGC CCTACTCCAG AGATTACAAG AGAAAGTACG AGTTCTTCAG AAGGAAGCTC 
AAGAAGCAGA CTGACATTCC AAACAAATTT GAAATGAAGC TTCGCCGCGC AAACATTCTG 
GAGGATTCTT ACCGGAGGAT TATGGGTGTG AAGAGAGCTG ACTTGCTCAA GGCCAGACTC 
TGGATTGAGT TTGATGGTGA AAAGGGCCTT GACTATGGAG GGGTTGCCAG AGAGTGGTTC 
TTCCTCATCT CGAAGGAAAT GTTCAACCCT TACTACGGCC TGTTTGAATA TTCTGCTACG 
GATAATTACA CCCTACAGAT AAATCCTAAC TCGGGCTTGT GTAATGAAGA TCACCTCTCA 
TACTTCAAGT TCATTGGCCG TGTGGCTGGG ATGGCAGTTT ATCATGGCAA GCTGTTGGAT 
GGGTTTTTCA TCCGTCCGTT TTACAAGATG ATGCTTCAGA AACTGATAAC ACTGCACGAC 
ATGGAGTCCG TGGATAGTGA ATATTACAGT TCTCTGCGAT GGATTCTTGA AAATGACCCG 
ACGGAGCTGG ACCTGAGATT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAGCAC 
GAACTGAAAA CCGGAGGATC AGAGATTGTT GTCACCAATA AGAACAAAAA GGAGTATATC 
TACCTTGTAA TACAATGGCG ATTTGTGAAC CGTATCCAGA AGCAAATGGC AGCTTTTAAA 
GAGGGATTTT TTGAACTGAT ACCACAGGAT CTCATCAAGA TATTTGATGA AAATGAGCTA 
GAGCTTCTCA TGTGTGGTCT GGGAGATGTG GATGTGAACG ACTGGCGGGA ACACACAAAA 
TACAAAAATG GCTACAGCAT GAACCACCAG GTCATCCACT GGTTCTGGAA GGCTGTTTGG 
ATGATGGATT CGGAAAAAAG AATACGCTTA CTTCAGTTTG TCACTGGCAC ATCCCGTGTG 
CCGATGAATG GGTTTGCTGA ACTCTATGGC TCGAATGGAC CACAATCCTT CACAGTGGAA 
CAATGGGGCA CCCCTGATAA GCTGCCAAGA GCACACACCT GCTTCAATCG CCTGGACCTG 
CCACCCTACG AATCCTTTGA CGAACTCTGG GATAAACTTC AGATGGCAAT TGAGAACACA 
CAGGGCTTTG ATGGCGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Large-Scale Quantitative Proteomics Identifies the Ubiquitin Ligase Nedd4-1 as an Essential Regulator of Liver Regeneration.
Developmental cell42(6)616-625.e8(2017 09)
Bachofner M,Speicher T,Bogorad RL,Muzumdar S,Derrer CP,H?rlimann F,B?hm F,Nanni P,Kockmann T,Kachaylo E,Meyer M,Padrissa-Alt?s S,Graf R,Anderson DG,Koteliansky V,Auf dem Keller U,Werner S

Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.
Proceedings of the National Academy of Sciences of the United States of America114(34)E7111-E7120(2017 Aug)
Li S,Mi L,Yu L,Yu Q,Liu T,Wang GX,Zhao XY,Wu J,Lin JD

The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy.
Autophagy13(12)2041-2055(2017)
Pei G,Buijze H,Liu H,Moura-Alves P,Goosmann C,Brinkmann V,Kawabe H,Dorhoi A,Kaufmann SHE

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Nedd4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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