Ncf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncf2
Entrez Gene ID	17970
Full Name	neutrophil cytosolic factor 2
Synonyms	NOXA2,Ncf-2,p67phox
General protein information	Preferred Names neutrophil cytosolic factor 2 Names neutrophil cytosol factor 2 67 kDa neutrophil oxidase factor NADPH oxidase activator 2 NADPH oxidase subunit (67kDa) neutrophil NADPH oxidase factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 G3\|1 65.05 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529236.4	XP_006529299.1	neutrophil cytosol factor 2 isoform X1
XM_006529236.3	XP_006529299.1	neutrophil cytosol factor 2 isoform X1
NM_010877.5	NP_035007.1	neutrophil cytosol factor 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NCF2	XP_001163353.1
Canis lupus familiaris (dog)	NCF2	NP_001095302.1
Mus musculus (house mouse)	Ncf2	NP_035007.1
Homo sapiens (human)	NCF2	NP_000424.2
Gallus gallus (chicken)	NCF2	XP_422288.2
Danio rerio (zebrafish)	ncf2	NP_001103932.1
Bos taurus (cattle)	NCF2	NP_776545.1
Rattus norvegicus (Norway rat)	Ncf2	NP_001094454.1
Xenopus tropicalis (tropical clawed frog)	ncf2	NP_001029119.1

Gene Ontology
Bibliography

Related articles in PubMed

Haploinsufficiency of NADPH Oxidase Subunit Neutrophil Cytosolic Factor 2 Is Sufficient to Accelerate Full-Blown Lupus in NZM 2328 Mice.
Jacob CO, Yu N, Yoo DG, Perez-Zapata LJ, Barbu EA, Kaplan MJ, Purmalek M, Pingel JT, Idol RA, Dinauer MC
Arthritis & rheumatology (Hoboken, N.J.)69(8)1647-1660(2017 08)

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

p67(phox) terminates the phospholipase A(2)-derived signal for activation of NADPH oxidase (NOX2).
Krishnaiah SY, Dodia C, Feinstein SI, Fisher AB
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(5)2066-73(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Just as patients with chronic granulomatous disease who lack NADPH oxidase rarely develop SLE, NCF-2-null mice on a nonautoimmune background were susceptible to a chronic granulomatous disease-like opportunistic infection but did not develop lupus. In contrast, on a lupus-prone background, even haploinsufficiency of NCF-2 accelerated the development of full-blown lupus disease.
Title: Haploinsufficiency of NADPH Oxidase Subunit Neutrophil Cytosolic Factor 2 Is Sufficient to Accelerate Full-Blown Lupus in NZM 2328 Mice.

p67(phox) binds to phosphoPrdx6 and inhibits its PLA2 activity, an interaction that could function to terminate the PLA2-mediated NOX2 activation signal.
Title: p67(phox) terminates the phospholipase A(2)-derived signal for activation of NADPH oxidase (NOX2).

The results of this study suggested that repeated stress promotes depressive behavior through the upregulation of NADPH oxidasesubunit (67kDa) and the resultant metabolic oxidative stress.
Title: NADPH oxidase mediates depressive behavior induced by chronic stress in mice.

Data suggest that a cytosolic source of reactive oxygen species, probably p67(phox) subunit of cardiac NADPH oxidase 2 (NOX2), may contribute to the hypertrophic phenotype in glucose transporter 4 gene (GLUT4-/-) mice.
Title: Cytosolic, but not mitochondrial, oxidative stress is a likely contributor to cardiac hypertrophy resulting from cardiac specific GLUT4 deletion in mice.

binding of the ternary complex to phagosomes can be temporally regulated by switching between adaptor proteins that have PX domains with distinct lipid-binding specificities
Title: Sequential binding of cytosolic Phox complex to phagosomes through regulated adaptor proteins: evaluation using the novel monomeric Kusabira-Green System and live imaging of phagocytosis.

The following Ncf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23077	NM_010877.5 Latest version!	Mus musculus neutrophil cytosolic factor 2 (Ncf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu23077	XM_006529236.4 Latest version!	Mus musculus neutrophil cytosolic factor 2 (Ncf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu23077	XM_006529236.3	Mus musculus neutrophil cytosolic factor 2 (Ncf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncf2 ( NM_010877.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035007.1 Mus musculus neutrophil cytosolic factor 2 (Ncf2), mRNA.

Price & Availability↑

CloneID	OMu23077
Clone ID Related Accession (Same CDS sequence)	XM_006529236.4 , XM_006529236.3 , NM_010877.5
Accession Version	NM_010877.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY097838.1, BC076609.1 and AK036379.1. On or before Aug 27, 2015 this sequence version replaced XM_006529235.2, NM_010877.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC076609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCTGG CTGAGGCCAT CAGACTCTGG AATGAAGGGG TGCTCGCAGC CGACAAGAAG 
GACTGGAAGG GTGCCCTGGA GGCCTTCAGC GAGGTGCAGG ACCCCCACTC GAGGATTTGC 
TTCAACATAG GCTGCGTGAA CACTATCCTG GAAAACTTGC AGGCAGCCGA GCAGGCCTTC 
ACCAAAAGCA TCAACAGAGA CAAGCACTCT GCAGTGGCCT ACTTCCAGAG AGGAATGCTC 
TACTACAGAA TGGAGAAGTA CGACCTTGCT ATCAAAGACC TTAAAGAGGC CTTGACGCAG 
CTTCGTGGGA ACCAGCTGAT AGACTACAAG ATCCTGGGGC TGCAGTTCAA GCTGTTTGCC 
TGTGAGGTAT TGTACAATAT TGCTCTCATG CATGCCAAGA AAGAGGAATG GAAGAAAGCA 
GAAGAGCAGT TGGCATTGGC AACCAACATG AAGTCCGAGC CCAGGCATTC CAAGATCGAC 
AAGGCCATGG AGAGCATCTG GAAGCAGAAG CTGTTCGAGC CCGTGGTGAT CCCTGTGGGT 
CGGCTGTTCC GTCCAAATGA GAGGCAGGTG GCTCAGCTGG CCAAAAAGGA CTATCTGGGC 
AAGGCTACGG TTGTAGCATC TGTGGTTCAC CAAGACAACT TTTCTGGCTT CGCCCCTCTG 
CAGCCACAGT CAGCAGAGCC TCCTCCCAGA CCCAAAACCC CAGAAATCTT CAGGGCTCTG 
GAAGGTGAGG CACACCGCGT ATTGTTTGGC TTTGTGCCGG AGACGCCAGA AGAGCTACAG 
GTCATGCCTG GGAACATCGT CTTTGTCTTG AAGAAGGGCA GTGATAACTG GGCCACAGTC 
ATGTTCAATG GACAGAAGGG GCTTGTCCCC TGCAACTACC TGGAGCCAGT TGAGCTTCGG 
ATTCACCCTC AGTCGCAGCC CCAGGAAGAT ACCTCTCCAG AATCCGATAT TCCACCACCT 
CCTAATTCTA GCCCCCCAGG AAGACTCCAG TTGTCACCAG GTCACAAGCA AAAAGAGCCC 
AAGGAACTGA AGCTCAGCGT GCCTATGCCT TACATGCTCA AGGTGCATTA CAAATACACA 
GTGGTCATGG AGACGCGGCT TGGCCTCCCC TACAGCCAGC TTCGGAACAT GGTGTCTAAG 
AAGCTGGCGC TCTCGCCAGA ACACACTAAA CTGAGCTACC GGCGTCGGGA CAGCCACGAG 
CTTCTGCTCC TGTCCGAAGA AAGCATGAAG GATGCCTGGG GCCAAGTGAA AAACTACTGC 
CTGACTCTGT GGTGTGAGCA TACGGTGGGT GACCAAGGTC TTATTGATGA ACCCATACAA 
AGGGAAAACT CAGACGCCAG TAAGCAGACT ACGGAGCCTC AGCCTAAGGA GGGGACCCAG 
GTGGTAGCAA TCTTCAGTTA TGAGGCTGCC CAGCCAGAAG ACCTGGAATT TGTGGAAGGA 
GATGTAATCC TGGTACTGTC ACATGTGAAT GAAGAATGGC TGGAAGGGGA GTGTAAAGGG 
AAAGTTGGCA TTTTCCCGAA GGCTTTTGTT GAAGGATGTG CAGCCAAGAA TTTGGAAGGC 
ATTCCCAGAG AAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035007.1
CDS	1137..2714
Translation	MSLAEAIRLWNEGVLAADKKDWKGALEAFSEVQDPHSRICFNIGCVNTILENLQAAEQAFTKSINRDKHSAVAYFQRGMLYYRMEKYDLAIKDLKEALTQLRGNQLIDYKILGLQFKLFACEVLYNIALMHAKKEEWKKAEEQLALATNMKSEPRHSKIDKAMESIWKQKLFEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVHQDNFSGFAPLQPQSAEPPPRPKTPEIFRALEGEAHRVLFGFVPETPEELQVMPGNIVFVLKKGSDNWATVMFNGQKGLVPCNYLEPVELRIHPQSQPQEDTSPESDIPPPPNSSPPGRLQLSPGHKQKEPKELKLSVPMPYMLKVHYKYTVVMETRLGLPYSQLRNMVSKKLALSPEHTKLSYRRRDSHELLLLSEESMKDAWGQVKNYCLTLWCEHTVGDQGLIDEPIQRENSDASKQTTEPQPKEGTQVVAIFSYEAAQPEDLEFVEGDVILVLSHVNEEWLEGECKGKVGIFPKAFVEGCAAKNLEGIPREV

Target ORF information:

RefSeq Version	NM_010877.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 2 (Ncf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010877.5

ORF Insert Sequence:

ATGTCCCTGG CTGAGGCCAT CAGACTCTGG AATGAAGGGG TGCTCGCAGC CGACAAGAAG 
GACTGGAAGG GTGCCCTGGA GGCCTTCAGC GAGGTGCAGG ACCCCCACTC GAGGATTTGC 
TTCAACATAG GCTGCGTGAA CACTATCCTG GAAAACTTGC AGGCAGCCGA GCAGGCCTTC 
ACCAAAAGCA TCAACAGAGA CAAGCACTCT GCAGTGGCCT ACTTCCAGAG AGGAATGCTC 
TACTACAGAA TGGAGAAGTA CGACCTTGCT ATCAAAGACC TTAAAGAGGC CTTGACGCAG 
CTTCGTGGGA ACCAGCTGAT AGACTACAAG ATCCTGGGGC TGCAGTTCAA GCTGTTTGCC 
TGTGAGGTAT TGTACAATAT TGCTCTCATG CATGCCAAGA AAGAGGAATG GAAGAAAGCA 
GAAGAGCAGT TGGCATTGGC AACCAACATG AAGTCCGAGC CCAGGCATTC CAAGATCGAC 
AAGGCCATGG AGAGCATCTG GAAGCAGAAG CTGTTCGAGC CCGTGGTGAT CCCTGTGGGT 
CGGCTGTTCC GTCCAAATGA GAGGCAGGTG GCTCAGCTGG CCAAAAAGGA CTATCTGGGC 
AAGGCTACGG TTGTAGCATC TGTGGTTCAC CAAGACAACT TTTCTGGCTT CGCCCCTCTG 
CAGCCACAGT CAGCAGAGCC TCCTCCCAGA CCCAAAACCC CAGAAATCTT CAGGGCTCTG 
GAAGGTGAGG CACACCGCGT ATTGTTTGGC TTTGTGCCGG AGACGCCAGA AGAGCTACAG 
GTCATGCCTG GGAACATCGT CTTTGTCTTG AAGAAGGGCA GTGATAACTG GGCCACAGTC 
ATGTTCAATG GACAGAAGGG GCTTGTCCCC TGCAACTACC TGGAGCCAGT TGAGCTTCGG 
ATTCACCCTC AGTCGCAGCC CCAGGAAGAT ACCTCTCCAG AATCCGATAT TCCACCACCT 
CCTAATTCTA GCCCCCCAGG AAGACTCCAG TTGTCACCAG GTCACAAGCA AAAAGAGCCC 
AAGGAACTGA AGCTCAGCGT GCCTATGCCT TACATGCTCA AGGTGCATTA CAAATACACA 
GTGGTCATGG AGACGCGGCT TGGCCTCCCC TACAGCCAGC TTCGGAACAT GGTGTCTAAG 
AAGCTGGCGC TCTCGCCAGA ACACACTAAA CTGAGCTACC GGCGTCGGGA CAGCCACGAG 
CTTCTGCTCC TGTCCGAAGA AAGCATGAAG GATGCCTGGG GCCAAGTGAA AAACTACTGC 
CTGACTCTGT GGTGTGAGCA TACGGTGGGT GACCAAGGTC TTATTGATGA ACCCATACAA 
AGGGAAAACT CAGACGCCAG TAAGCAGACT ACGGAGCCTC AGCCTAAGGA GGGGACCCAG 
GTGGTAGCAA TCTTCAGTTA TGAGGCTGCC CAGCCAGAAG ACCTGGAATT TGTGGAAGGA 
GATGTAATCC TGGTACTGTC ACATGTGAAT GAAGAATGGC TGGAAGGGGA GTGTAAAGGG 
AAAGTTGGCA TTTTCCCGAA GGCTTTTGTT GAAGGATGTG CAGCCAAGAA TTTGGAAGGC 
ATTCCCAGAG AAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncf2 ( XM_006529236.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529299.1 Mus musculus neutrophil cytosolic factor 2 (Ncf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23077
Clone ID Related Accession (Same CDS sequence)	XM_006529236.4 , XM_006529236.3 , NM_010877.5
Accession Version	XM_006529236.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGG CTGAGGCCAT CAGACTCTGG AATGAAGGGG TGCTCGCAGC CGACAAGAAG 
GACTGGAAGG GTGCCCTGGA GGCCTTCAGC GAGGTGCAGG ACCCCCACTC GAGGATTTGC 
TTCAACATAG GCTGCGTGAA CACTATCCTG GAAAACTTGC AGGCAGCCGA GCAGGCCTTC 
ACCAAAAGCA TCAACAGAGA CAAGCACTCT GCAGTGGCCT ACTTCCAGAG AGGAATGCTC 
TACTACAGAA TGGAGAAGTA CGACCTTGCT ATCAAAGACC TTAAAGAGGC CTTGACGCAG 
CTTCGTGGGA ACCAGCTGAT AGACTACAAG ATCCTGGGGC TGCAGTTCAA GCTGTTTGCC 
TGTGAGGTAT TGTACAATAT TGCTCTCATG CATGCCAAGA AAGAGGAATG GAAGAAAGCA 
GAAGAGCAGT TGGCATTGGC AACCAACATG AAGTCCGAGC CCAGGCATTC CAAGATCGAC 
AAGGCCATGG AGAGCATCTG GAAGCAGAAG CTGTTCGAGC CCGTGGTGAT CCCTGTGGGT 
CGGCTGTTCC GTCCAAATGA GAGGCAGGTG GCTCAGCTGG CCAAAAAGGA CTATCTGGGC 
AAGGCTACGG TTGTAGCATC TGTGGTTCAC CAAGACAACT TTTCTGGCTT CGCCCCTCTG 
CAGCCACAGT CAGCAGAGCC TCCTCCCAGA CCCAAAACCC CAGAAATCTT CAGGGCTCTG 
GAAGGTGAGG CACACCGCGT ATTGTTTGGC TTTGTGCCGG AGACGCCAGA AGAGCTACAG 
GTCATGCCTG GGAACATCGT CTTTGTCTTG AAGAAGGGCA GTGATAACTG GGCCACAGTC 
ATGTTCAATG GACAGAAGGG GCTTGTCCCC TGCAACTACC TGGAGCCAGT TGAGCTTCGG 
ATTCACCCTC AGTCGCAGCC CCAGGAAGAT ACCTCTCCAG AATCCGATAT TCCACCACCT 
CCTAATTCTA GCCCCCCAGG AAGACTCCAG TTGTCACCAG GTCACAAGCA AAAAGAGCCC 
AAGGAACTGA AGCTCAGCGT GCCTATGCCT TACATGCTCA AGGTGCATTA CAAATACACA 
GTGGTCATGG AGACGCGGCT TGGCCTCCCC TACAGCCAGC TTCGGAACAT GGTGTCTAAG 
AAGCTGGCGC TCTCGCCAGA ACACACTAAA CTGAGCTACC GGCGTCGGGA CAGCCACGAG 
CTTCTGCTCC TGTCCGAAGA AAGCATGAAG GATGCCTGGG GCCAAGTGAA AAACTACTGC 
CTGACTCTGT GGTGTGAGCA TACGGTGGGT GACCAAGGTC TTATTGATGA ACCCATACAA 
AGGGAAAACT CAGACGCCAG TAAGCAGACT ACGGAGCCTC AGCCTAAGGA GGGGACCCAG 
GTGGTAGCAA TCTTCAGTTA TGAGGCTGCC CAGCCAGAAG ACCTGGAATT TGTGGAAGGA 
GATGTAATCC TGGTACTGTC ACATGTGAAT GAAGAATGGC TGGAAGGGGA GTGTAAAGGG 
AAAGTTGGCA TTTTCCCGAA GGCTTTTGTT GAAGGATGTG CAGCCAAGAA TTTGGAAGGC 
ATTCCCAGAG AAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529299.1
CDS	676..2253
Translation	MSLAEAIRLWNEGVLAADKKDWKGALEAFSEVQDPHSRICFNIGCVNTILENLQAAEQAFTKSINRDKHSAVAYFQRGMLYYRMEKYDLAIKDLKEALTQLRGNQLIDYKILGLQFKLFACEVLYNIALMHAKKEEWKKAEEQLALATNMKSEPRHSKIDKAMESIWKQKLFEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVHQDNFSGFAPLQPQSAEPPPRPKTPEIFRALEGEAHRVLFGFVPETPEELQVMPGNIVFVLKKGSDNWATVMFNGQKGLVPCNYLEPVELRIHPQSQPQEDTSPESDIPPPPNSSPPGRLQLSPGHKQKEPKELKLSVPMPYMLKVHYKYTVVMETRLGLPYSQLRNMVSKKLALSPEHTKLSYRRRDSHELLLLSEESMKDAWGQVKNYCLTLWCEHTVGDQGLIDEPIQRENSDASKQTTEPQPKEGTQVVAIFSYEAAQPEDLEFVEGDVILVLSHVNEEWLEGECKGKVGIFPKAFVEGCAAKNLEGIPREV

Target ORF information:

RefSeq Version	XM_006529236.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 2 (Ncf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529236.3

ORF Insert Sequence:

ATGTCCCTGG CTGAGGCCAT CAGACTCTGG AATGAAGGGG TGCTCGCAGC CGACAAGAAG 
GACTGGAAGG GTGCCCTGGA GGCCTTCAGC GAGGTGCAGG ACCCCCACTC GAGGATTTGC 
TTCAACATAG GCTGCGTGAA CACTATCCTG GAAAACTTGC AGGCAGCCGA GCAGGCCTTC 
ACCAAAAGCA TCAACAGAGA CAAGCACTCT GCAGTGGCCT ACTTCCAGAG AGGAATGCTC 
TACTACAGAA TGGAGAAGTA CGACCTTGCT ATCAAAGACC TTAAAGAGGC CTTGACGCAG 
CTTCGTGGGA ACCAGCTGAT AGACTACAAG ATCCTGGGGC TGCAGTTCAA GCTGTTTGCC 
TGTGAGGTAT TGTACAATAT TGCTCTCATG CATGCCAAGA AAGAGGAATG GAAGAAAGCA 
GAAGAGCAGT TGGCATTGGC AACCAACATG AAGTCCGAGC CCAGGCATTC CAAGATCGAC 
AAGGCCATGG AGAGCATCTG GAAGCAGAAG CTGTTCGAGC CCGTGGTGAT CCCTGTGGGT 
CGGCTGTTCC GTCCAAATGA GAGGCAGGTG GCTCAGCTGG CCAAAAAGGA CTATCTGGGC 
AAGGCTACGG TTGTAGCATC TGTGGTTCAC CAAGACAACT TTTCTGGCTT CGCCCCTCTG 
CAGCCACAGT CAGCAGAGCC TCCTCCCAGA CCCAAAACCC CAGAAATCTT CAGGGCTCTG 
GAAGGTGAGG CACACCGCGT ATTGTTTGGC TTTGTGCCGG AGACGCCAGA AGAGCTACAG 
GTCATGCCTG GGAACATCGT CTTTGTCTTG AAGAAGGGCA GTGATAACTG GGCCACAGTC 
ATGTTCAATG GACAGAAGGG GCTTGTCCCC TGCAACTACC TGGAGCCAGT TGAGCTTCGG 
ATTCACCCTC AGTCGCAGCC CCAGGAAGAT ACCTCTCCAG AATCCGATAT TCCACCACCT 
CCTAATTCTA GCCCCCCAGG AAGACTCCAG TTGTCACCAG GTCACAAGCA AAAAGAGCCC 
AAGGAACTGA AGCTCAGCGT GCCTATGCCT TACATGCTCA AGGTGCATTA CAAATACACA 
GTGGTCATGG AGACGCGGCT TGGCCTCCCC TACAGCCAGC TTCGGAACAT GGTGTCTAAG 
AAGCTGGCGC TCTCGCCAGA ACACACTAAA CTGAGCTACC GGCGTCGGGA CAGCCACGAG 
CTTCTGCTCC TGTCCGAAGA AAGCATGAAG GATGCCTGGG GCCAAGTGAA AAACTACTGC 
CTGACTCTGT GGTGTGAGCA TACGGTGGGT GACCAAGGTC TTATTGATGA ACCCATACAA 
AGGGAAAACT CAGACGCCAG TAAGCAGACT ACGGAGCCTC AGCCTAAGGA GGGGACCCAG 
GTGGTAGCAA TCTTCAGTTA TGAGGCTGCC CAGCCAGAAG ACCTGGAATT TGTGGAAGGA 
GATGTAATCC TGGTACTGTC ACATGTGAAT GAAGAATGGC TGGAAGGGGA GTGTAAAGGG 
AAAGTTGGCA TTTTCCCGAA GGCTTTTGTT GAAGGATGTG CAGCCAAGAA TTTGGAAGGC 
ATTCCCAGAG AAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncf2 ( XM_006529236.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529299.1 Mus musculus neutrophil cytosolic factor 2 (Ncf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23077
Clone ID Related Accession (Same CDS sequence)	XM_006529236.4 , XM_006529236.3 , NM_010877.5
Accession Version	XM_006529236.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neutrophil cytosol factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529236.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGG CTGAGGCCAT CAGACTCTGG AATGAAGGGG TGCTCGCAGC CGACAAGAAG 
GACTGGAAGG GTGCCCTGGA GGCCTTCAGC GAGGTGCAGG ACCCCCACTC GAGGATTTGC 
TTCAACATAG GCTGCGTGAA CACTATCCTG GAAAACTTGC AGGCAGCCGA GCAGGCCTTC 
ACCAAAAGCA TCAACAGAGA CAAGCACTCT GCAGTGGCCT ACTTCCAGAG AGGAATGCTC 
TACTACAGAA TGGAGAAGTA CGACCTTGCT ATCAAAGACC TTAAAGAGGC CTTGACGCAG 
CTTCGTGGGA ACCAGCTGAT AGACTACAAG ATCCTGGGGC TGCAGTTCAA GCTGTTTGCC 
TGTGAGGTAT TGTACAATAT TGCTCTCATG CATGCCAAGA AAGAGGAATG GAAGAAAGCA 
GAAGAGCAGT TGGCATTGGC AACCAACATG AAGTCCGAGC CCAGGCATTC CAAGATCGAC 
AAGGCCATGG AGAGCATCTG GAAGCAGAAG CTGTTCGAGC CCGTGGTGAT CCCTGTGGGT 
CGGCTGTTCC GTCCAAATGA GAGGCAGGTG GCTCAGCTGG CCAAAAAGGA CTATCTGGGC 
AAGGCTACGG TTGTAGCATC TGTGGTTCAC CAAGACAACT TTTCTGGCTT CGCCCCTCTG 
CAGCCACAGT CAGCAGAGCC TCCTCCCAGA CCCAAAACCC CAGAAATCTT CAGGGCTCTG 
GAAGGTGAGG CACACCGCGT ATTGTTTGGC TTTGTGCCGG AGACGCCAGA AGAGCTACAG 
GTCATGCCTG GGAACATCGT CTTTGTCTTG AAGAAGGGCA GTGATAACTG GGCCACAGTC 
ATGTTCAATG GACAGAAGGG GCTTGTCCCC TGCAACTACC TGGAGCCAGT TGAGCTTCGG 
ATTCACCCTC AGTCGCAGCC CCAGGAAGAT ACCTCTCCAG AATCCGATAT TCCACCACCT 
CCTAATTCTA GCCCCCCAGG AAGACTCCAG TTGTCACCAG GTCACAAGCA AAAAGAGCCC 
AAGGAACTGA AGCTCAGCGT GCCTATGCCT TACATGCTCA AGGTGCATTA CAAATACACA 
GTGGTCATGG AGACGCGGCT TGGCCTCCCC TACAGCCAGC TTCGGAACAT GGTGTCTAAG 
AAGCTGGCGC TCTCGCCAGA ACACACTAAA CTGAGCTACC GGCGTCGGGA CAGCCACGAG 
CTTCTGCTCC TGTCCGAAGA AAGCATGAAG GATGCCTGGG GCCAAGTGAA AAACTACTGC 
CTGACTCTGT GGTGTGAGCA TACGGTGGGT GACCAAGGTC TTATTGATGA ACCCATACAA 
AGGGAAAACT CAGACGCCAG TAAGCAGACT ACGGAGCCTC AGCCTAAGGA GGGGACCCAG 
GTGGTAGCAA TCTTCAGTTA TGAGGCTGCC CAGCCAGAAG ACCTGGAATT TGTGGAAGGA 
GATGTAATCC TGGTACTGTC ACATGTGAAT GAAGAATGGC TGGAAGGGGA GTGTAAAGGG 
AAAGTTGGCA TTTTCCCGAA GGCTTTTGTT GAAGGATGTG CAGCCAAGAA TTTGGAAGGC 
ATTCCCAGAG AAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529299.1
CDS	614..2191
Translation	MSLAEAIRLWNEGVLAADKKDWKGALEAFSEVQDPHSRICFNIGCVNTILENLQAAEQAFTKSINRDKHSAVAYFQRGMLYYRMEKYDLAIKDLKEALTQLRGNQLIDYKILGLQFKLFACEVLYNIALMHAKKEEWKKAEEQLALATNMKSEPRHSKIDKAMESIWKQKLFEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVHQDNFSGFAPLQPQSAEPPPRPKTPEIFRALEGEAHRVLFGFVPETPEELQVMPGNIVFVLKKGSDNWATVMFNGQKGLVPCNYLEPVELRIHPQSQPQEDTSPESDIPPPPNSSPPGRLQLSPGHKQKEPKELKLSVPMPYMLKVHYKYTVVMETRLGLPYSQLRNMVSKKLALSPEHTKLSYRRRDSHELLLLSEESMKDAWGQVKNYCLTLWCEHTVGDQGLIDEPIQRENSDASKQTTEPQPKEGTQVVAIFSYEAAQPEDLEFVEGDVILVLSHVNEEWLEGECKGKVGIFPKAFVEGCAAKNLEGIPREV

Target ORF information:

RefSeq Version	XM_006529236.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neutrophil cytosolic factor 2 (Ncf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529236.4

ORF Insert Sequence:

ATGTCCCTGG CTGAGGCCAT CAGACTCTGG AATGAAGGGG TGCTCGCAGC CGACAAGAAG 
GACTGGAAGG GTGCCCTGGA GGCCTTCAGC GAGGTGCAGG ACCCCCACTC GAGGATTTGC 
TTCAACATAG GCTGCGTGAA CACTATCCTG GAAAACTTGC AGGCAGCCGA GCAGGCCTTC 
ACCAAAAGCA TCAACAGAGA CAAGCACTCT GCAGTGGCCT ACTTCCAGAG AGGAATGCTC 
TACTACAGAA TGGAGAAGTA CGACCTTGCT ATCAAAGACC TTAAAGAGGC CTTGACGCAG 
CTTCGTGGGA ACCAGCTGAT AGACTACAAG ATCCTGGGGC TGCAGTTCAA GCTGTTTGCC 
TGTGAGGTAT TGTACAATAT TGCTCTCATG CATGCCAAGA AAGAGGAATG GAAGAAAGCA 
GAAGAGCAGT TGGCATTGGC AACCAACATG AAGTCCGAGC CCAGGCATTC CAAGATCGAC 
AAGGCCATGG AGAGCATCTG GAAGCAGAAG CTGTTCGAGC CCGTGGTGAT CCCTGTGGGT 
CGGCTGTTCC GTCCAAATGA GAGGCAGGTG GCTCAGCTGG CCAAAAAGGA CTATCTGGGC 
AAGGCTACGG TTGTAGCATC TGTGGTTCAC CAAGACAACT TTTCTGGCTT CGCCCCTCTG 
CAGCCACAGT CAGCAGAGCC TCCTCCCAGA CCCAAAACCC CAGAAATCTT CAGGGCTCTG 
GAAGGTGAGG CACACCGCGT ATTGTTTGGC TTTGTGCCGG AGACGCCAGA AGAGCTACAG 
GTCATGCCTG GGAACATCGT CTTTGTCTTG AAGAAGGGCA GTGATAACTG GGCCACAGTC 
ATGTTCAATG GACAGAAGGG GCTTGTCCCC TGCAACTACC TGGAGCCAGT TGAGCTTCGG 
ATTCACCCTC AGTCGCAGCC CCAGGAAGAT ACCTCTCCAG AATCCGATAT TCCACCACCT 
CCTAATTCTA GCCCCCCAGG AAGACTCCAG TTGTCACCAG GTCACAAGCA AAAAGAGCCC 
AAGGAACTGA AGCTCAGCGT GCCTATGCCT TACATGCTCA AGGTGCATTA CAAATACACA 
GTGGTCATGG AGACGCGGCT TGGCCTCCCC TACAGCCAGC TTCGGAACAT GGTGTCTAAG 
AAGCTGGCGC TCTCGCCAGA ACACACTAAA CTGAGCTACC GGCGTCGGGA CAGCCACGAG 
CTTCTGCTCC TGTCCGAAGA AAGCATGAAG GATGCCTGGG GCCAAGTGAA AAACTACTGC 
CTGACTCTGT GGTGTGAGCA TACGGTGGGT GACCAAGGTC TTATTGATGA ACCCATACAA 
AGGGAAAACT CAGACGCCAG TAAGCAGACT ACGGAGCCTC AGCCTAAGGA GGGGACCCAG 
GTGGTAGCAA TCTTCAGTTA TGAGGCTGCC CAGCCAGAAG ACCTGGAATT TGTGGAAGGA 
GATGTAATCC TGGTACTGTC ACATGTGAAT GAAGAATGGC TGGAAGGGGA GTGTAAAGGG 
AAAGTTGGCA TTTTCCCGAA GGCTTTTGTT GAAGGATGTG CAGCCAAGAA TTTGGAAGGC 
ATTCCCAGAG AAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Haploinsufficiency of NADPH Oxidase Subunit Neutrophil Cytosolic Factor 2 Is Sufficient to Accelerate Full-Blown Lupus in NZM 2328 Mice.
Arthritis & rheumatology (Hoboken, N.J.)69(8)1647-1660(2017 08)
Jacob CO,Yu N,Yoo DG,Perez-Zapata LJ,Barbu EA,Kaplan MJ,Purmalek M,Pingel JT,Idol RA,Dinauer MC

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

p67(phox) terminates the phospholipase A(2)-derived signal for activation of NADPH oxidase (NOX2).
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(5)2066-73(2013 May)
Krishnaiah SY,Dodia C,Feinstein SI,Fisher AB

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Ncf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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