Myo1b gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006495764.3	XP_006495827.1	unconventional myosin-Ib isoform X1
XM_006495767.3	XP_006495830.1	unconventional myosin-Ib isoform X4
NM_001290982.1	NP_001277911.1	unconventional myosin-Ib isoform 3
XM_006495764.2	XP_006495827.1	unconventional myosin-Ib isoform X1
XM_030252028.1	XP_030107888.1	unconventional myosin-Ib isoform X2
NM_001161817.2	NP_001155289.1	unconventional myosin-Ib isoform 1
XM_006495765.3	XP_006495828.1	unconventional myosin-Ib isoform X1
XM_017319213.1	XP_017174702.1	unconventional myosin-Ib isoform X6
NM_010863.4	NP_034993.2	unconventional myosin-Ib isoform 2
XM_006495766.2	XP_006495829.1	unconventional myosin-Ib isoform X2
XM_006495767.2	XP_006495830.1	unconventional myosin-Ib isoform X3
XM_006495766.3	XP_006495829.1	unconventional myosin-Ib isoform X3
XM_006495769.3	XP_006495832.1	unconventional myosin-Ib isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77014	XM_017319213.1 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu14407	NM_001161817.2 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$664.30 $949.00
OMu14407	XM_030252028.1 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu27294	XM_006495764.3 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu27295	XM_006495766.3 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu27296	XM_006495767.3 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu14397	NM_010863.4 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$650.30 $929.00
OMu16932	NM_001290982.1 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu27294	XM_006495765.3 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu27297	XM_006495769.3 Latest version!	Mus musculus myosin IB (Myo1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu27294	XM_006495764.2	Mus musculus myosin IB (Myo1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu27296	XM_006495767.2	Mus musculus myosin IB (Myo1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu27295	XM_006495766.2	Mus musculus myosin IB (Myo1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

Myo1b ( XM_017319213.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174702.1 Mus musculus myosin IB (Myo1b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu77014
Clone ID Related Accession (Same CDS sequence)	XM_017319213.1
Accession Version	XM_017319213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TCGCTGCCGT GTGCGGAAAA GGAGCAGAAG TCAATCAGGT CAAGGAACAG 
CTTCTGCAGT CCAACCCTGT CCTGGAAGCT TTTGGAAATG CCAAGACTGT GAGGAATGAC 
AACTCATCTA GATTCGGCAA ATACATGGAT ATTGAATTTG ACTTTAAAGG CGATCCGCTG 
GGAGGAGTGA TAAGTAACTA TCTTTTAGAG AAATCTCGTG TTGTTAAGCA GCCAAGAGGT 
GAAAGAAACT TCCATGTGTT CTATCAGCTG CTCTCAGGCG CCTCTGAGGA GCTCCTCTAC 
AAGCTTAAGC TGGAGCGGGA TTTCAGCAGA TACAACTATC TGAGTCTGGA CTCTGCTAAA 
GTTAATGGTG TGGATGATGC TGCCAATTTC AGAACTGTTA GGAATGCGAT GCAGATCGTG 
GGCTTTCTGG ATCATGAGGC CGAGGCTGTC CTGGAGGTAG TGGCAGCCGT TTTGAAACTG 
GGGAACATCG AGTTCAAGCC TGAATCACGA GTGAATGGTT TGGATGAAAG CAAAATCAAA 
GATAAAAATG AGTTAAAAGA AATTTGCGAG TTGACCAGCA TCGATCAGGT GGTTTTAGAA 
AGAGCGTTCA GTTTCCGAAC AGTTGAAGCG AAGCAGGAGA AAGTTTCAAC CACGCTGAAT 
GTGGCTCAGG CTTATTATGC CCGTGATGCC CTGGCTAAAA ACCTCTACAG CCGCTTGTTT 
TCATGGTTGG TAAATCGTAT CAACGAAAGC ATTAAGGCAC AAACAAAAGT GAGAAAGAAG 
GTCATGGGTG TTCTGGACAT TTATGGCTTT GAAATATTTG AGGATAACAG CTTCGAGCAG 
TTTATTATTA ACTATTGTAA TGAAAAGCTT CAACAAATCT TCATCGAACT TACCCTCAAA 
GAAGAGCAAG AGGAATATAT TCGGGAGGAC ATAGAATGGA CTCACATTGA CTACTTCAAC 
AATGCTATCA TTTGTGACCT AATAGAAAAT AACACAAATG GAATCTTGGC CATGCTGGAT 
GAAGAGTGCC TGAGACCCGG CACTGTTACA GATGAGACCT TCCTGGAAAA GCTGAACCAA 
GTCTGTGCCA CCCACCAGCA CTTTGAGAGC AGGATGAGCA AGTGTTCCCG CTTTCTCAAC 
GACACAACCC TGCCCCACAG CTGCTTCAGA ATCCAGCATT ATGCTGGCAA GGTGCTCTAC 
CAGGTGGAAG GATTTGTTGA CAAGAACAAT GACCTTCTGT ACCGGGACCT GTCTCAAGCC 
ATGTGGAAGG CCGGCCACTC CCTCATCAAG TCTCTGTTTC CTGAAGGAAA TCCTGCAAAG 
GTCAACCTGA AGAGACCTCC CACCGCAGGC TCCCAGTTCA AGGCGTCTGT GGCCACGCTG 
ATGAGAAACC TGCAGACCAA GAACCCGAAC TACATCAGGT GCATCAAGCC AAATGATAAA 
AAGGCAGCTC ACATTTTCAA TGAGAGTCTC GTATGCCATC AGATCAGGTA CCTGGGTCTT 
TTGGAGAACG TTCGAGTGAG ACGGGCAGGC TATGCCTTCA GGCAGGCCTA TGAACCTTGC 
CTGGAAAGAT ACAAGATGCT TTGCAAACAA ACGTGGCCTC ACTGGAAGGG ACCAGCAAGG 
TCTGGCGTGG AGGTTCTGTT TAATGAGCTA GAGATTCCTG TGGAAGAGCA CTCCTTCGGG 
AGATCCAAGA TATTCATCCG AAACCCACGG ACATTATTCC AACTAGAAGA CCTAAGGAAG 
CAGCGGCTGG AGGACCTGGC CACTCTCATT CAGAAGATTT ATCGAGGATG GAAATGCCGT 
ACACATTTTC TGCTCATGAA AAGAAGCCAA GTTGTGATTG CTGCCTGGTA CAGGAGATAT 
GCGCAACAAA AGCGGTACCA GCAGATAAAG AGTTCAGCCC TGGTGATTCA GTCGTACATC 
CGGGGCTGGA AGGCTCGAAA AATCCTGCGG GAGCTGAAGC ATCAGAAGCG CTGTAAGGAG 
GCAGCCACCA CCATCGCGGC GTACTGGCAT GGCACCCAGG CACGGAGGGA GTTGAAACGC 
TTGAAGGAGG AGGCTAGGCG TAAGCATGCA GTTGCTGTCA TTTGGGCTTA CTGGCTTGGA 
CTGAAGGTAC GCAGGGAATA CAGGAAATTC TTTAGAGCAA ATGCTGGAAA GAAAATCTAT 
GAGTTTACAC TTCAAAGAAT TGTGCAAAAA TACTTGTTGG AAATGAAAAA TAAAATGCCT 
TCCTTGTCAC CGATAGATAA AAATTGGCCA TCAAGACCTT ACCTGTTCTT GGATTCGACT 
CACAAGGAGC TAAAAAGGAT CTTCCACTTG TGGAGGTGTA AAAAATACAG GGATCAATTC 
ACAGACCAGC AGAAACTTAT TTATGAAGAG AAGCTCGAGG CCAGCGAACT CTTCAAAGAC 
AAGAAGGCTT TATATCCTTC TAGTGTTGGG CAACCATTCC AAGGAGCTTA CCTGGAAATC 
AACAAGAACC CAAAATATAA GAAACTCAAA GATGCCATTG AAGAGAAGAT CATTATTGCT 
GAGGTTGTGA ACAAAATTAA TCGGGCTAAT GGGAAGAGTA CATCTCGGAT TTTCCTCTTA 
ACAAACAATA ACCTTCTCCT TGCTGACCAA AAGTCTGGGC AGATCAAGTC CGAGGTCCCC 
CTGGTAGATG TGACCAAGGT TTCCATGAGT TCCCAGAACG ATGGTTTCTT TGCAGTGCAC 
CTCAAGGAGG GTTCAGAAGC GGCTAGTAAA GGGGACTTTC TCTTCAGCAG TGACCACCTG 
ATTGAAATGG CAACCAAGCT GTATCGCACG ACTCTCAGCC AAACTAAACA GAAGCTCAAC 
ATCGAGATTT CCGATGAGTT CCTGGTACAG TTCAGACAGG ACAAAGTATG TGTGAAGTTT 
ATTCAAGGCA ACCAGAAAAA TGGGAGCGTG CCAACCTGCA AACGAAAGAA CAACAGACTC 
CTTGAAGTCG CTGTCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174702.1
CDS	507..3467
Translation	MSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_017319213.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319213.1

ORF Insert Sequence:

ATGTCCTATG TCGCTGCCGT GTGCGGAAAA GGAGCAGAAG TCAATCAGGT CAAGGAACAG 
CTTCTGCAGT CCAACCCTGT CCTGGAAGCT TTTGGAAATG CCAAGACTGT GAGGAATGAC 
AACTCATCTA GATTCGGCAA ATACATGGAT ATTGAATTTG ACTTTAAAGG CGATCCGCTG 
GGAGGAGTGA TAAGTAACTA TCTTTTAGAG AAATCTCGTG TTGTTAAGCA GCCAAGAGGT 
GAAAGAAACT TCCATGTGTT CTATCAGCTG CTCTCAGGCG CCTCTGAGGA GCTCCTCTAC 
AAGCTTAAGC TGGAGCGGGA TTTCAGCAGA TACAACTATC TGAGTCTGGA CTCTGCTAAA 
GTTAATGGTG TGGATGATGC TGCCAATTTC AGAACTGTTA GGAATGCGAT GCAGATCGTG 
GGCTTTCTGG ATCATGAGGC CGAGGCTGTC CTGGAGGTAG TGGCAGCCGT TTTGAAACTG 
GGGAACATCG AGTTCAAGCC TGAATCACGA GTGAATGGTT TGGATGAAAG CAAAATCAAA 
GATAAAAATG AGTTAAAAGA AATTTGCGAG TTGACCAGCA TCGATCAGGT GGTTTTAGAA 
AGAGCGTTCA GTTTCCGAAC AGTTGAAGCG AAGCAGGAGA AAGTTTCAAC CACGCTGAAT 
GTGGCTCAGG CTTATTATGC CCGTGATGCC CTGGCTAAAA ACCTCTACAG CCGCTTGTTT 
TCATGGTTGG TAAATCGTAT CAACGAAAGC ATTAAGGCAC AAACAAAAGT GAGAAAGAAG 
GTCATGGGTG TTCTGGACAT TTATGGCTTT GAAATATTTG AGGATAACAG CTTCGAGCAG 
TTTATTATTA ACTATTGTAA TGAAAAGCTT CAACAAATCT TCATCGAACT TACCCTCAAA 
GAAGAGCAAG AGGAATATAT TCGGGAGGAC ATAGAATGGA CTCACATTGA CTACTTCAAC 
AATGCTATCA TTTGTGACCT AATAGAAAAT AACACAAATG GAATCTTGGC CATGCTGGAT 
GAAGAGTGCC TGAGACCCGG CACTGTTACA GATGAGACCT TCCTGGAAAA GCTGAACCAA 
GTCTGTGCCA CCCACCAGCA CTTTGAGAGC AGGATGAGCA AGTGTTCCCG CTTTCTCAAC 
GACACAACCC TGCCCCACAG CTGCTTCAGA ATCCAGCATT ATGCTGGCAA GGTGCTCTAC 
CAGGTGGAAG GATTTGTTGA CAAGAACAAT GACCTTCTGT ACCGGGACCT GTCTCAAGCC 
ATGTGGAAGG CCGGCCACTC CCTCATCAAG TCTCTGTTTC CTGAAGGAAA TCCTGCAAAG 
GTCAACCTGA AGAGACCTCC CACCGCAGGC TCCCAGTTCA AGGCGTCTGT GGCCACGCTG 
ATGAGAAACC TGCAGACCAA GAACCCGAAC TACATCAGGT GCATCAAGCC AAATGATAAA 
AAGGCAGCTC ACATTTTCAA TGAGAGTCTC GTATGCCATC AGATCAGGTA CCTGGGTCTT 
TTGGAGAACG TTCGAGTGAG ACGGGCAGGC TATGCCTTCA GGCAGGCCTA TGAACCTTGC 
CTGGAAAGAT ACAAGATGCT TTGCAAACAA ACGTGGCCTC ACTGGAAGGG ACCAGCAAGG 
TCTGGCGTGG AGGTTCTGTT TAATGAGCTA GAGATTCCTG TGGAAGAGCA CTCCTTCGGG 
AGATCCAAGA TATTCATCCG AAACCCACGG ACATTATTCC AACTAGAAGA CCTAAGGAAG 
CAGCGGCTGG AGGACCTGGC CACTCTCATT CAGAAGATTT ATCGAGGATG GAAATGCCGT 
ACACATTTTC TGCTCATGAA AAGAAGCCAA GTTGTGATTG CTGCCTGGTA CAGGAGATAT 
GCGCAACAAA AGCGGTACCA GCAGATAAAG AGTTCAGCCC TGGTGATTCA GTCGTACATC 
CGGGGCTGGA AGGCTCGAAA AATCCTGCGG GAGCTGAAGC ATCAGAAGCG CTGTAAGGAG 
GCAGCCACCA CCATCGCGGC GTACTGGCAT GGCACCCAGG CACGGAGGGA GTTGAAACGC 
TTGAAGGAGG AGGCTAGGCG TAAGCATGCA GTTGCTGTCA TTTGGGCTTA CTGGCTTGGA 
CTGAAGGTAC GCAGGGAATA CAGGAAATTC TTTAGAGCAA ATGCTGGAAA GAAAATCTAT 
GAGTTTACAC TTCAAAGAAT TGTGCAAAAA TACTTGTTGG AAATGAAAAA TAAAATGCCT 
TCCTTGTCAC CGATAGATAA AAATTGGCCA TCAAGACCTT ACCTGTTCTT GGATTCGACT 
CACAAGGAGC TAAAAAGGAT CTTCCACTTG TGGAGGTGTA AAAAATACAG GGATCAATTC 
ACAGACCAGC AGAAACTTAT TTATGAAGAG AAGCTCGAGG CCAGCGAACT CTTCAAAGAC 
AAGAAGGCTT TATATCCTTC TAGTGTTGGG CAACCATTCC AAGGAGCTTA CCTGGAAATC 
AACAAGAACC CAAAATATAA GAAACTCAAA GATGCCATTG AAGAGAAGAT CATTATTGCT 
GAGGTTGTGA ACAAAATTAA TCGGGCTAAT GGGAAGAGTA CATCTCGGAT TTTCCTCTTA 
ACAAACAATA ACCTTCTCCT TGCTGACCAA AAGTCTGGGC AGATCAAGTC CGAGGTCCCC 
CTGGTAGATG TGACCAAGGT TTCCATGAGT TCCCAGAACG ATGGTTTCTT TGCAGTGCAC 
CTCAAGGAGG GTTCAGAAGC GGCTAGTAAA GGGGACTTTC TCTTCAGCAG TGACCACCTG 
ATTGAAATGG CAACCAAGCT GTATCGCACG ACTCTCAGCC AAACTAAACA GAAGCTCAAC 
ATCGAGATTT CCGATGAGTT CCTGGTACAG TTCAGACAGG ACAAAGTATG TGTGAAGTTT 
ATTCAAGGCA ACCAGAAAAA TGGGAGCGTG CCAACCTGCA AACGAAAGAA CAACAGACTC 
CTTGAAGTCG CTGTCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( NM_001161817.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155289.1 Mus musculus myosin IB (Myo1b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14407
Clone ID Related Accession (Same CDS sequence)	XM_030252028.1 , NM_001161817.2
Accession Version	NM_001161817.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108840.13, BC054786.1, BY759345.1, BE950809.1 and BB768105.1. On Mar 21, 2014 this sequence version replaced NM_001161817.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGCGCGAAG GGAGCTGAGA 
CGGCTGAAGG ACGAGGCTAG GAATAAGCAC GCTATTGCAG TTATTTGGGC TTACTGGCTT 
GGATCGAAGG CACGGAGGGA GTTGAAACGC TTGAAGGAGG AGGCTAGGCG TAAGCATGCA 
GTTGCTGTCA TTTGGGCTTA CTGGCTTGGA CTGAAGGTAC GCAGGGAATA CAGGAAATTC 
TTTAGAGCAA ATGCTGGAAA GAAAATCTAT GAGTTTACAC TTCAAAGAAT TGTGCAAAAA 
TACTTGTTGG AAATGAAAAA TAAAATGCCT TCCTTGTCAC CGATAGATAA AAATTGGCCA 
TCAAGACCTT ACCTGTTCTT GGATTCGACT CACAAGGAGC TAAAAAGGAT CTTCCACTTG 
TGGAGGTGTA AAAAATACAG GGATCAATTC ACAGACCAGC AGAAACTTAT TTATGAAGAG 
AAGCTCGAGG CCAGCGAACT CTTCAAAGAC AAGAAGGCTT TATATCCTTC TAGTGTTGGG 
CAACCATTCC AAGGAGCTTA CCTGGAAATC AACAAGAACC CAAAATATAA GAAACTCAAA 
GATGCCATTG AAGAGAAGAT CATTATTGCT GAGGTTGTGA ACAAAATTAA TCGGGCTAAT 
GGGAAGAGTA CATCTCGGAT TTTCCTCTTA ACAAACAATA ACCTTCTCCT TGCTGACCAA 
AAGTCTGGGC AGATCAAGTC CGAGGTCCCC CTGGTAGATG TGACCAAGGT TTCCATGAGT 
TCCCAGAACG ATGGTTTCTT TGCAGTGCAC CTCAAGGAGG GTTCAGAAGC GGCTAGTAAA 
GGGGACTTTC TCTTCAGCAG TGACCACCTG ATTGAAATGG CAACCAAGCT GTATCGCACG 
ACTCTCAGCC AAACTAAACA GAAGCTCAAC ATCGAGATTT CCGATGAGTT CCTGGTACAG 
TTCAGACAGG ACAAAGTATG TGTGAAGTTT ATTCAAGGCA ACCAGAAAAA TGGGAGCGTG 
CCAACCTGCA AACGAAAGAA CAACAGACTC CTTGAAGTCG CTGTCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155289.1
CDS	228..3638
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELRRLKDEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	NM_001161817.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161817.2

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGCGCGAAG GGAGCTGAGA 
CGGCTGAAGG ACGAGGCTAG GAATAAGCAC GCTATTGCAG TTATTTGGGC TTACTGGCTT 
GGATCGAAGG CACGGAGGGA GTTGAAACGC TTGAAGGAGG AGGCTAGGCG TAAGCATGCA 
GTTGCTGTCA TTTGGGCTTA CTGGCTTGGA CTGAAGGTAC GCAGGGAATA CAGGAAATTC 
TTTAGAGCAA ATGCTGGAAA GAAAATCTAT GAGTTTACAC TTCAAAGAAT TGTGCAAAAA 
TACTTGTTGG AAATGAAAAA TAAAATGCCT TCCTTGTCAC CGATAGATAA AAATTGGCCA 
TCAAGACCTT ACCTGTTCTT GGATTCGACT CACAAGGAGC TAAAAAGGAT CTTCCACTTG 
TGGAGGTGTA AAAAATACAG GGATCAATTC ACAGACCAGC AGAAACTTAT TTATGAAGAG 
AAGCTCGAGG CCAGCGAACT CTTCAAAGAC AAGAAGGCTT TATATCCTTC TAGTGTTGGG 
CAACCATTCC AAGGAGCTTA CCTGGAAATC AACAAGAACC CAAAATATAA GAAACTCAAA 
GATGCCATTG AAGAGAAGAT CATTATTGCT GAGGTTGTGA ACAAAATTAA TCGGGCTAAT 
GGGAAGAGTA CATCTCGGAT TTTCCTCTTA ACAAACAATA ACCTTCTCCT TGCTGACCAA 
AAGTCTGGGC AGATCAAGTC CGAGGTCCCC CTGGTAGATG TGACCAAGGT TTCCATGAGT 
TCCCAGAACG ATGGTTTCTT TGCAGTGCAC CTCAAGGAGG GTTCAGAAGC GGCTAGTAAA 
GGGGACTTTC TCTTCAGCAG TGACCACCTG ATTGAAATGG CAACCAAGCT GTATCGCACG 
ACTCTCAGCC AAACTAAACA GAAGCTCAAC ATCGAGATTT CCGATGAGTT CCTGGTACAG 
TTCAGACAGG ACAAAGTATG TGTGAAGTTT ATTCAAGGCA ACCAGAAAAA TGGGAGCGTG 
CCAACCTGCA AACGAAAGAA CAACAGACTC CTTGAAGTCG CTGTCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_030252028.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107888.1 Mus musculus myosin IB (Myo1b), transcript variant X3, mRNA.

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CloneID	OMu14407
Clone ID Related Accession (Same CDS sequence)	XM_030252028.1 , NM_001161817.2
Accession Version	XM_030252028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGCGCGAAG GGAGCTGAGA 
CGGCTGAAGG ACGAGGCTAG GAATAAGCAC GCTATTGCAG TTATTTGGGC TTACTGGCTT 
GGATCGAAGG CACGGAGGGA GTTGAAACGC TTGAAGGAGG AGGCTAGGCG TAAGCATGCA 
GTTGCTGTCA TTTGGGCTTA CTGGCTTGGA CTGAAGGTAC GCAGGGAATA CAGGAAATTC 
TTTAGAGCAA ATGCTGGAAA GAAAATCTAT GAGTTTACAC TTCAAAGAAT TGTGCAAAAA 
TACTTGTTGG AAATGAAAAA TAAAATGCCT TCCTTGTCAC CGATAGATAA AAATTGGCCA 
TCAAGACCTT ACCTGTTCTT GGATTCGACT CACAAGGAGC TAAAAAGGAT CTTCCACTTG 
TGGAGGTGTA AAAAATACAG GGATCAATTC ACAGACCAGC AGAAACTTAT TTATGAAGAG 
AAGCTCGAGG CCAGCGAACT CTTCAAAGAC AAGAAGGCTT TATATCCTTC TAGTGTTGGG 
CAACCATTCC AAGGAGCTTA CCTGGAAATC AACAAGAACC CAAAATATAA GAAACTCAAA 
GATGCCATTG AAGAGAAGAT CATTATTGCT GAGGTTGTGA ACAAAATTAA TCGGGCTAAT 
GGGAAGAGTA CATCTCGGAT TTTCCTCTTA ACAAACAATA ACCTTCTCCT TGCTGACCAA 
AAGTCTGGGC AGATCAAGTC CGAGGTCCCC CTGGTAGATG TGACCAAGGT TTCCATGAGT 
TCCCAGAACG ATGGTTTCTT TGCAGTGCAC CTCAAGGAGG GTTCAGAAGC GGCTAGTAAA 
GGGGACTTTC TCTTCAGCAG TGACCACCTG ATTGAAATGG CAACCAAGCT GTATCGCACG 
ACTCTCAGCC AAACTAAACA GAAGCTCAAC ATCGAGATTT CCGATGAGTT CCTGGTACAG 
TTCAGACAGG ACAAAGTATG TGTGAAGTTT ATTCAAGGCA ACCAGAAAAA TGGGAGCGTG 
CCAACCTGCA AACGAAAGAA CAACAGACTC CTTGAAGTCG CTGTCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107888.1
CDS	900..4310
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELRRLKDEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_030252028.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252028.1

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGCGCGAAG GGAGCTGAGA 
CGGCTGAAGG ACGAGGCTAG GAATAAGCAC GCTATTGCAG TTATTTGGGC TTACTGGCTT 
GGATCGAAGG CACGGAGGGA GTTGAAACGC TTGAAGGAGG AGGCTAGGCG TAAGCATGCA 
GTTGCTGTCA TTTGGGCTTA CTGGCTTGGA CTGAAGGTAC GCAGGGAATA CAGGAAATTC 
TTTAGAGCAA ATGCTGGAAA GAAAATCTAT GAGTTTACAC TTCAAAGAAT TGTGCAAAAA 
TACTTGTTGG AAATGAAAAA TAAAATGCCT TCCTTGTCAC CGATAGATAA AAATTGGCCA 
TCAAGACCTT ACCTGTTCTT GGATTCGACT CACAAGGAGC TAAAAAGGAT CTTCCACTTG 
TGGAGGTGTA AAAAATACAG GGATCAATTC ACAGACCAGC AGAAACTTAT TTATGAAGAG 
AAGCTCGAGG CCAGCGAACT CTTCAAAGAC AAGAAGGCTT TATATCCTTC TAGTGTTGGG 
CAACCATTCC AAGGAGCTTA CCTGGAAATC AACAAGAACC CAAAATATAA GAAACTCAAA 
GATGCCATTG AAGAGAAGAT CATTATTGCT GAGGTTGTGA ACAAAATTAA TCGGGCTAAT 
GGGAAGAGTA CATCTCGGAT TTTCCTCTTA ACAAACAATA ACCTTCTCCT TGCTGACCAA 
AAGTCTGGGC AGATCAAGTC CGAGGTCCCC CTGGTAGATG TGACCAAGGT TTCCATGAGT 
TCCCAGAACG ATGGTTTCTT TGCAGTGCAC CTCAAGGAGG GTTCAGAAGC GGCTAGTAAA 
GGGGACTTTC TCTTCAGCAG TGACCACCTG ATTGAAATGG CAACCAAGCT GTATCGCACG 
ACTCTCAGCC AAACTAAACA GAAGCTCAAC ATCGAGATTT CCGATGAGTT CCTGGTACAG 
TTCAGACAGG ACAAAGTATG TGTGAAGTTT ATTCAAGGCA ACCAGAAAAA TGGGAGCGTG 
CCAACCTGCA AACGAAAGAA CAACAGACTC CTTGAAGTCG CTGTCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495764.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495827.1 Mus musculus myosin IB (Myo1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27294
Clone ID Related Accession (Same CDS sequence)	XM_006495764.3 , XM_006495764.2 , XM_006495765.3
Accession Version	XM_006495764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC GCGAAGGGAG 
CTGAGACGGC TGAAGGACGA GGCTAGGAAT AAGCACGCTA TTGCAGTTAT TTGGGCTTAC 
TGGCTTGGAT CGAAGGCACG GAGGGAGTTG AAACGCTTGA AGGAGGAGGC TAGGCGTAAG 
CATGCAGTTG CTGTCATTTG GGCTTACTGG CTTGGACTGA AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495827.1
CDS	313..3729
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELRRLKDEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495764.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495764.3

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC GCGAAGGGAG 
CTGAGACGGC TGAAGGACGA GGCTAGGAAT AAGCACGCTA TTGCAGTTAT TTGGGCTTAC 
TGGCTTGGAT CGAAGGCACG GAGGGAGTTG AAACGCTTGA AGGAGGAGGC TAGGCGTAAG 
CATGCAGTTG CTGTCATTTG GGCTTACTGG CTTGGACTGA AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495766.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495829.1 Mus musculus myosin IB (Myo1b), transcript variant X4, mRNA.

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CloneID	OMu27295
Clone ID Related Accession (Same CDS sequence)	XM_006495766.2 , XM_006495766.3
Accession Version	XM_006495766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC ACGGAGGGAG 
TTGAAACGCT TGAAGGAGGA GGCTAGGCGT AAGCATGCAG TTGCTGTCAT TTGGGCTTAC 
TGGCTTGGAC TGAAGGTACG CAGGGAATAC AGGAAATTCT TTAGAGCAAA TGCTGGAAAG 
AAAATCTATG AGTTTACACT TCAAAGAATT GTGCAAAAAT ACTTGTTGGA AATGAAAAAT 
AAAATGCCTT CCTTGTCACC GATAGATAAA AATTGGCCAT CAAGACCTTA CCTGTTCTTG 
GATTCGACTC ACAAGGAGCT AAAAAGGATC TTCCACTTGT GGAGGTGTAA AAAATACAGG 
GATCAATTCA CAGACCAGCA GAAACTTATT TATGAAGAGA AGCTCGAGGC CAGCGAACTC 
TTCAAAGACA AGAAGGCTTT ATATCCTTCT AGTGTTGGGC AACCATTCCA AGGAGCTTAC 
CTGGAAATCA ACAAGAACCC AAAATATAAG AAACTCAAAG ATGCCATTGA AGAGAAGATC 
ATTATTGCTG AGGTTGTGAA CAAAATTAAT CGGGCTAATG GGAAGAGTAC ATCTCGGATT 
TTCCTCTTAA CAAACAATAA CCTTCTCCTT GCTGACCAAA AGTCTGGGCA GATCAAGTCC 
GAGGTCCCCC TGGTAGATGT GACCAAGGTT TCCATGAGTT CCCAGAACGA TGGTTTCTTT 
GCAGTGCACC TCAAGGAGGG TTCAGAAGCG GCTAGTAAAG GGGACTTTCT CTTCAGCAGT 
GACCACCTGA TTGAAATGGC AACCAAGCTG TATCGCACGA CTCTCAGCCA AACTAAACAG 
AAGCTCAACA TCGAGATTTC CGATGAGTTC CTGGTACAGT TCAGACAGGA CAAAGTATGT 
GTGAAGTTTA TTCAAGGCAA CCAGAAAAAT GGGAGCGTGC CAACCTGCAA ACGAAAGAAC 
AACAGACTCC TTGAAGTCGC TGTCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495829.1
CDS	313..3642
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495766.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495766.3

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC ACGGAGGGAG 
TTGAAACGCT TGAAGGAGGA GGCTAGGCGT AAGCATGCAG TTGCTGTCAT TTGGGCTTAC 
TGGCTTGGAC TGAAGGTACG CAGGGAATAC AGGAAATTCT TTAGAGCAAA TGCTGGAAAG 
AAAATCTATG AGTTTACACT TCAAAGAATT GTGCAAAAAT ACTTGTTGGA AATGAAAAAT 
AAAATGCCTT CCTTGTCACC GATAGATAAA AATTGGCCAT CAAGACCTTA CCTGTTCTTG 
GATTCGACTC ACAAGGAGCT AAAAAGGATC TTCCACTTGT GGAGGTGTAA AAAATACAGG 
GATCAATTCA CAGACCAGCA GAAACTTATT TATGAAGAGA AGCTCGAGGC CAGCGAACTC 
TTCAAAGACA AGAAGGCTTT ATATCCTTCT AGTGTTGGGC AACCATTCCA AGGAGCTTAC 
CTGGAAATCA ACAAGAACCC AAAATATAAG AAACTCAAAG ATGCCATTGA AGAGAAGATC 
ATTATTGCTG AGGTTGTGAA CAAAATTAAT CGGGCTAATG GGAAGAGTAC ATCTCGGATT 
TTCCTCTTAA CAAACAATAA CCTTCTCCTT GCTGACCAAA AGTCTGGGCA GATCAAGTCC 
GAGGTCCCCC TGGTAGATGT GACCAAGGTT TCCATGAGTT CCCAGAACGA TGGTTTCTTT 
GCAGTGCACC TCAAGGAGGG TTCAGAAGCG GCTAGTAAAG GGGACTTTCT CTTCAGCAGT 
GACCACCTGA TTGAAATGGC AACCAAGCTG TATCGCACGA CTCTCAGCCA AACTAAACAG 
AAGCTCAACA TCGAGATTTC CGATGAGTTC CTGGTACAGT TCAGACAGGA CAAAGTATGT 
GTGAAGTTTA TTCAAGGCAA CCAGAAAAAT GGGAGCGTGC CAACCTGCAA ACGAAAGAAC 
AACAGACTCC TTGAAGTCGC TGTCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495767.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495830.1 Mus musculus myosin IB (Myo1b), transcript variant X5, mRNA.

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CloneID	OMu27296
Clone ID Related Accession (Same CDS sequence)	XM_006495767.3 , XM_006495767.2
Accession Version	XM_006495767.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGT ACGCAGGGAA 
TACAGGAAAT TCTTTAGAGC AAATGCTGGA AAGAAAATCT ATGAGTTTAC ACTTCAAAGA 
ATTGTGCAAA AATACTTGTT GGAAATGAAA AATAAAATGC CTTCCTTGTC ACCGATAGAT 
AAAAATTGGC CATCAAGACC TTACCTGTTC TTGGATTCGA CTCACAAGGA GCTAAAAAGG 
ATCTTCCACT TGTGGAGGTG TAAAAAATAC AGGGATCAAT TCACAGACCA GCAGAAACTT 
ATTTATGAAG AGAAGCTCGA GGCCAGCGAA CTCTTCAAAG ACAAGAAGGC TTTATATCCT 
TCTAGTGTTG GGCAACCATT CCAAGGAGCT TACCTGGAAA TCAACAAGAA CCCAAAATAT 
AAGAAACTCA AAGATGCCAT TGAAGAGAAG ATCATTATTG CTGAGGTTGT GAACAAAATT 
AATCGGGCTA ATGGGAAGAG TACATCTCGG ATTTTCCTCT TAACAAACAA TAACCTTCTC 
CTTGCTGACC AAAAGTCTGG GCAGATCAAG TCCGAGGTCC CCCTGGTAGA TGTGACCAAG 
GTTTCCATGA GTTCCCAGAA CGATGGTTTC TTTGCAGTGC ACCTCAAGGA GGGTTCAGAA 
GCGGCTAGTA AAGGGGACTT TCTCTTCAGC AGTGACCACC TGATTGAAAT GGCAACCAAG 
CTGTATCGCA CGACTCTCAG CCAAACTAAA CAGAAGCTCA ACATCGAGAT TTCCGATGAG 
TTCCTGGTAC AGTTCAGACA GGACAAAGTA TGTGTGAAGT TTATTCAAGG CAACCAGAAA 
AATGGGAGCG TGCCAACCTG CAAACGAAAG AACAACAGAC TCCTTGAAGT CGCTGTCCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495830.1
CDS	313..3555
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495767.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495767.3

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGT ACGCAGGGAA 
TACAGGAAAT TCTTTAGAGC AAATGCTGGA AAGAAAATCT ATGAGTTTAC ACTTCAAAGA 
ATTGTGCAAA AATACTTGTT GGAAATGAAA AATAAAATGC CTTCCTTGTC ACCGATAGAT 
AAAAATTGGC CATCAAGACC TTACCTGTTC TTGGATTCGA CTCACAAGGA GCTAAAAAGG 
ATCTTCCACT TGTGGAGGTG TAAAAAATAC AGGGATCAAT TCACAGACCA GCAGAAACTT 
ATTTATGAAG AGAAGCTCGA GGCCAGCGAA CTCTTCAAAG ACAAGAAGGC TTTATATCCT 
TCTAGTGTTG GGCAACCATT CCAAGGAGCT TACCTGGAAA TCAACAAGAA CCCAAAATAT 
AAGAAACTCA AAGATGCCAT TGAAGAGAAG ATCATTATTG CTGAGGTTGT GAACAAAATT 
AATCGGGCTA ATGGGAAGAG TACATCTCGG ATTTTCCTCT TAACAAACAA TAACCTTCTC 
CTTGCTGACC AAAAGTCTGG GCAGATCAAG TCCGAGGTCC CCCTGGTAGA TGTGACCAAG 
GTTTCCATGA GTTCCCAGAA CGATGGTTTC TTTGCAGTGC ACCTCAAGGA GGGTTCAGAA 
GCGGCTAGTA AAGGGGACTT TCTCTTCAGC AGTGACCACC TGATTGAAAT GGCAACCAAG 
CTGTATCGCA CGACTCTCAG CCAAACTAAA CAGAAGCTCA ACATCGAGAT TTCCGATGAG 
TTCCTGGTAC AGTTCAGACA GGACAAAGTA TGTGTGAAGT TTATTCAAGG CAACCAGAAA 
AATGGGAGCG TGCCAACCTG CAAACGAAAG AACAACAGAC TCCTTGAAGT CGCTGTCCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( NM_010863.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034993.2 Mus musculus myosin IB (Myo1b), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14397
Clone ID Related Accession (Same CDS sequence)	NM_010863.4
Accession Version	NM_010863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108840.13, AK141207.1, BC054786.1, BY759345.1, BE950809.1 and BB768105.1. On Mar 21, 2014 this sequence version replaced NM_010863.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK141207.1, BC046300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGCACGGAG GGAGTTGAAA 
CGCTTGAAGG AGGAGGCTAG GCGTAAGCAT GCAGTTGCTG TCATTTGGGC TTACTGGCTT 
GGACTGAAGG TACGCAGGGA ATACAGGAAA TTCTTTAGAG CAAATGCTGG AAAGAAAATC 
TATGAGTTTA CACTTCAAAG AATTGTGCAA AAATACTTGT TGGAAATGAA AAATAAAATG 
CCTTCCTTGT CACCGATAGA TAAAAATTGG CCATCAAGAC CTTACCTGTT CTTGGATTCG 
ACTCACAAGG AGCTAAAAAG GATCTTCCAC TTGTGGAGGT GTAAAAAATA CAGGGATCAA 
TTCACAGACC AGCAGAAACT TATTTATGAA GAGAAGCTCG AGGCCAGCGA ACTCTTCAAA 
GACAAGAAGG CTTTATATCC TTCTAGTGTT GGGCAACCAT TCCAAGGAGC TTACCTGGAA 
ATCAACAAGA ACCCAAAATA TAAGAAACTC AAAGATGCCA TTGAAGAGAA GATCATTATT 
GCTGAGGTTG TGAACAAAAT TAATCGGGCT AATGGGAAGA GTACATCTCG GATTTTCCTC 
TTAACAAACA ATAACCTTCT CCTTGCTGAC CAAAAGTCTG GGCAGATCAA GTCCGAGGTC 
CCCCTGGTAG ATGTGACCAA GGTTTCCATG AGTTCCCAGA ACGATGGTTT CTTTGCAGTG 
CACCTCAAGG AGGGTTCAGA AGCGGCTAGT AAAGGGGACT TTCTCTTCAG CAGTGACCAC 
CTGATTGAAA TGGCAACCAA GCTGTATCGC ACGACTCTCA GCCAAACTAA ACAGAAGCTC 
AACATCGAGA TTTCCGATGA GTTCCTGGTA CAGTTCAGAC AGGACAAAGT ATGTGTGAAG 
TTTATTCAAG GCAACCAGAA AAATGGGAGC GTGCCAACCT GCAAACGAAA GAACAACAGA 
CTCCTTGAAG TCGCTGTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034993.2
CDS	228..3551
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	NM_010863.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010863.4

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGCACGGAG GGAGTTGAAA 
CGCTTGAAGG AGGAGGCTAG GCGTAAGCAT GCAGTTGCTG TCATTTGGGC TTACTGGCTT 
GGACTGAAGG TACGCAGGGA ATACAGGAAA TTCTTTAGAG CAAATGCTGG AAAGAAAATC 
TATGAGTTTA CACTTCAAAG AATTGTGCAA AAATACTTGT TGGAAATGAA AAATAAAATG 
CCTTCCTTGT CACCGATAGA TAAAAATTGG CCATCAAGAC CTTACCTGTT CTTGGATTCG 
ACTCACAAGG AGCTAAAAAG GATCTTCCAC TTGTGGAGGT GTAAAAAATA CAGGGATCAA 
TTCACAGACC AGCAGAAACT TATTTATGAA GAGAAGCTCG AGGCCAGCGA ACTCTTCAAA 
GACAAGAAGG CTTTATATCC TTCTAGTGTT GGGCAACCAT TCCAAGGAGC TTACCTGGAA 
ATCAACAAGA ACCCAAAATA TAAGAAACTC AAAGATGCCA TTGAAGAGAA GATCATTATT 
GCTGAGGTTG TGAACAAAAT TAATCGGGCT AATGGGAAGA GTACATCTCG GATTTTCCTC 
TTAACAAACA ATAACCTTCT CCTTGCTGAC CAAAAGTCTG GGCAGATCAA GTCCGAGGTC 
CCCCTGGTAG ATGTGACCAA GGTTTCCATG AGTTCCCAGA ACGATGGTTT CTTTGCAGTG 
CACCTCAAGG AGGGTTCAGA AGCGGCTAGT AAAGGGGACT TTCTCTTCAG CAGTGACCAC 
CTGATTGAAA TGGCAACCAA GCTGTATCGC ACGACTCTCA GCCAAACTAA ACAGAAGCTC 
AACATCGAGA TTTCCGATGA GTTCCTGGTA CAGTTCAGAC AGGACAAAGT ATGTGTGAAG 
TTTATTCAAG GCAACCAGAA AAATGGGAGC GTGCCAACCT GCAAACGAAA GAACAACAGA 
CTCCTTGAAG TCGCTGTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( NM_001290982.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277911.1 Mus musculus myosin IB (Myo1b), transcript variant 3, mRNA.

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CloneID	OMu16932
Clone ID Related Accession (Same CDS sequence)	NM_001290982.1
Accession Version	NM_001290982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC108840.13, AK161062.1, BC054786.1, BY759345.1, BE950809.1 and BB768105.1. On Mar 21, 2014 this sequence version replaced XM_006495768.1. Transcript Variant: This variant (3) lacks two in-frame exons in the 3' coding region, compared to variant 1. The encoded protein (isoform 3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277911.1
CDS	228..3464
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	NM_001290982.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290982.1

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGGATAA CAGCTTCGAG 
CAGTTTATTA TTAACTATTG TAATGAAAAG CTTCAACAAA TCTTCATCGA ACTTACCCTC 
AAAGAAGAGC AAGAGGAATA TATTCGGGAG GACATAGAAT GGACTCACAT TGACTACTTC 
AACAATGCTA TCATTTGTGA CCTAATAGAA AATAACACAA ATGGAATCTT GGCCATGCTG 
GATGAAGAGT GCCTGAGACC CGGCACTGTT ACAGATGAGA CCTTCCTGGA AAAGCTGAAC 
CAAGTCTGTG CCACCCACCA GCACTTTGAG AGCAGGATGA GCAAGTGTTC CCGCTTTCTC 
AACGACACAA CCCTGCCCCA CAGCTGCTTC AGAATCCAGC ATTATGCTGG CAAGGTGCTC 
TACCAGGTGG AAGGATTTGT TGACAAGAAC AATGACCTTC TGTACCGGGA CCTGTCTCAA 
GCCATGTGGA AGGCCGGCCA CTCCCTCATC AAGTCTCTGT TTCCTGAAGG AAATCCTGCA 
AAGGTCAACC TGAAGAGACC TCCCACCGCA GGCTCCCAGT TCAAGGCGTC TGTGGCCACG 
CTGATGAGAA ACCTGCAGAC CAAGAACCCG AACTACATCA GGTGCATCAA GCCAAATGAT 
AAAAAGGCAG CTCACATTTT CAATGAGAGT CTCGTATGCC ATCAGATCAG GTACCTGGGT 
CTTTTGGAGA ACGTTCGAGT GAGACGGGCA GGCTATGCCT TCAGGCAGGC CTATGAACCT 
TGCCTGGAAA GATACAAGAT GCTTTGCAAA CAAACGTGGC CTCACTGGAA GGGACCAGCA 
AGGTCTGGCG TGGAGGTTCT GTTTAATGAG CTAGAGATTC CTGTGGAAGA GCACTCCTTC 
GGGAGATCCA AGATATTCAT CCGAAACCCA CGGACATTAT TCCAACTAGA AGACCTAAGG 
AAGCAGCGGC TGGAGGACCT GGCCACTCTC ATTCAGAAGA TTTATCGAGG ATGGAAATGC 
CGTACACATT TTCTGCTCAT GAAAAGAAGC CAAGTTGTGA TTGCTGCCTG GTACAGGAGA 
TATGCGCAAC AAAAGCGGTA CCAGCAGATA AAGAGTTCAG CCCTGGTGAT TCAGTCGTAC 
ATCCGGGGCT GGAAGGCTCG AAAAATCCTG CGGGAGCTGA AGCATCAGAA GCGCTGTAAG 
GAGGCAGCCA CCACCATCGC GGCGTACTGG CATGGCACCC AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495764.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495827.1 Mus musculus myosin IB (Myo1b), transcript variant X1, mRNA.

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CloneID	OMu27294
Clone ID Related Accession (Same CDS sequence)	XM_006495764.3 , XM_006495764.2 , XM_006495765.3
Accession Version	XM_006495764.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC GCGAAGGGAG 
CTGAGACGGC TGAAGGACGA GGCTAGGAAT AAGCACGCTA TTGCAGTTAT TTGGGCTTAC 
TGGCTTGGAT CGAAGGCACG GAGGGAGTTG AAACGCTTGA AGGAGGAGGC TAGGCGTAAG 
CATGCAGTTG CTGTCATTTG GGCTTACTGG CTTGGACTGA AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495827.1
CDS	223..3639
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELRRLKDEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495764.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495764.2

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC GCGAAGGGAG 
CTGAGACGGC TGAAGGACGA GGCTAGGAAT AAGCACGCTA TTGCAGTTAT TTGGGCTTAC 
TGGCTTGGAT CGAAGGCACG GAGGGAGTTG AAACGCTTGA AGGAGGAGGC TAGGCGTAAG 
CATGCAGTTG CTGTCATTTG GGCTTACTGG CTTGGACTGA AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495765.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495828.1 Mus musculus myosin IB (Myo1b), transcript variant X2, mRNA.

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CloneID	OMu27294
Clone ID Related Accession (Same CDS sequence)	XM_006495764.3 , XM_006495764.2 , XM_006495765.3
Accession Version	XM_006495765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC GCGAAGGGAG 
CTGAGACGGC TGAAGGACGA GGCTAGGAAT AAGCACGCTA TTGCAGTTAT TTGGGCTTAC 
TGGCTTGGAT CGAAGGCACG GAGGGAGTTG AAACGCTTGA AGGAGGAGGC TAGGCGTAAG 
CATGCAGTTG CTGTCATTTG GGCTTACTGG CTTGGACTGA AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495828.1
CDS	901..4317
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELRRLKDEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495765.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495765.3

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC GCGAAGGGAG 
CTGAGACGGC TGAAGGACGA GGCTAGGAAT AAGCACGCTA TTGCAGTTAT TTGGGCTTAC 
TGGCTTGGAT CGAAGGCACG GAGGGAGTTG AAACGCTTGA AGGAGGAGGC TAGGCGTAAG 
CATGCAGTTG CTGTCATTTG GGCTTACTGG CTTGGACTGA AGGTACGCAG GGAATACAGG 
AAATTCTTTA GAGCAAATGC TGGAAAGAAA ATCTATGAGT TTACACTTCA AAGAATTGTG 
CAAAAATACT TGTTGGAAAT GAAAAATAAA ATGCCTTCCT TGTCACCGAT AGATAAAAAT 
TGGCCATCAA GACCTTACCT GTTCTTGGAT TCGACTCACA AGGAGCTAAA AAGGATCTTC 
CACTTGTGGA GGTGTAAAAA ATACAGGGAT CAATTCACAG ACCAGCAGAA ACTTATTTAT 
GAAGAGAAGC TCGAGGCCAG CGAACTCTTC AAAGACAAGA AGGCTTTATA TCCTTCTAGT 
GTTGGGCAAC CATTCCAAGG AGCTTACCTG GAAATCAACA AGAACCCAAA ATATAAGAAA 
CTCAAAGATG CCATTGAAGA GAAGATCATT ATTGCTGAGG TTGTGAACAA AATTAATCGG 
GCTAATGGGA AGAGTACATC TCGGATTTTC CTCTTAACAA ACAATAACCT TCTCCTTGCT 
GACCAAAAGT CTGGGCAGAT CAAGTCCGAG GTCCCCCTGG TAGATGTGAC CAAGGTTTCC 
ATGAGTTCCC AGAACGATGG TTTCTTTGCA GTGCACCTCA AGGAGGGTTC AGAAGCGGCT 
AGTAAAGGGG ACTTTCTCTT CAGCAGTGAC CACCTGATTG AAATGGCAAC CAAGCTGTAT 
CGCACGACTC TCAGCCAAAC TAAACAGAAG CTCAACATCG AGATTTCCGA TGAGTTCCTG 
GTACAGTTCA GACAGGACAA AGTATGTGTG AAGTTTATTC AAGGCAACCA GAAAAATGGG 
AGCGTGCCAA CCTGCAAACG AAAGAACAAC AGACTCCTTG AAGTCGCTGT CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495766.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495829.1 Mus musculus myosin IB (Myo1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu27295
Clone ID Related Accession (Same CDS sequence)	XM_006495766.2 , XM_006495766.3
Accession Version	XM_006495766.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC ACGGAGGGAG 
TTGAAACGCT TGAAGGAGGA GGCTAGGCGT AAGCATGCAG TTGCTGTCAT TTGGGCTTAC 
TGGCTTGGAC TGAAGGTACG CAGGGAATAC AGGAAATTCT TTAGAGCAAA TGCTGGAAAG 
AAAATCTATG AGTTTACACT TCAAAGAATT GTGCAAAAAT ACTTGTTGGA AATGAAAAAT 
AAAATGCCTT CCTTGTCACC GATAGATAAA AATTGGCCAT CAAGACCTTA CCTGTTCTTG 
GATTCGACTC ACAAGGAGCT AAAAAGGATC TTCCACTTGT GGAGGTGTAA AAAATACAGG 
GATCAATTCA CAGACCAGCA GAAACTTATT TATGAAGAGA AGCTCGAGGC CAGCGAACTC 
TTCAAAGACA AGAAGGCTTT ATATCCTTCT AGTGTTGGGC AACCATTCCA AGGAGCTTAC 
CTGGAAATCA ACAAGAACCC AAAATATAAG AAACTCAAAG ATGCCATTGA AGAGAAGATC 
ATTATTGCTG AGGTTGTGAA CAAAATTAAT CGGGCTAATG GGAAGAGTAC ATCTCGGATT 
TTCCTCTTAA CAAACAATAA CCTTCTCCTT GCTGACCAAA AGTCTGGGCA GATCAAGTCC 
GAGGTCCCCC TGGTAGATGT GACCAAGGTT TCCATGAGTT CCCAGAACGA TGGTTTCTTT 
GCAGTGCACC TCAAGGAGGG TTCAGAAGCG GCTAGTAAAG GGGACTTTCT CTTCAGCAGT 
GACCACCTGA TTGAAATGGC AACCAAGCTG TATCGCACGA CTCTCAGCCA AACTAAACAG 
AAGCTCAACA TCGAGATTTC CGATGAGTTC CTGGTACAGT TCAGACAGGA CAAAGTATGT 
GTGAAGTTTA TTCAAGGCAA CCAGAAAAAT GGGAGCGTGC CAACCTGCAA ACGAAAGAAC 
AACAGACTCC TTGAAGTCGC TGTCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495829.1
CDS	223..3552
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495766.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495766.2

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGC ACGGAGGGAG 
TTGAAACGCT TGAAGGAGGA GGCTAGGCGT AAGCATGCAG TTGCTGTCAT TTGGGCTTAC 
TGGCTTGGAC TGAAGGTACG CAGGGAATAC AGGAAATTCT TTAGAGCAAA TGCTGGAAAG 
AAAATCTATG AGTTTACACT TCAAAGAATT GTGCAAAAAT ACTTGTTGGA AATGAAAAAT 
AAAATGCCTT CCTTGTCACC GATAGATAAA AATTGGCCAT CAAGACCTTA CCTGTTCTTG 
GATTCGACTC ACAAGGAGCT AAAAAGGATC TTCCACTTGT GGAGGTGTAA AAAATACAGG 
GATCAATTCA CAGACCAGCA GAAACTTATT TATGAAGAGA AGCTCGAGGC CAGCGAACTC 
TTCAAAGACA AGAAGGCTTT ATATCCTTCT AGTGTTGGGC AACCATTCCA AGGAGCTTAC 
CTGGAAATCA ACAAGAACCC AAAATATAAG AAACTCAAAG ATGCCATTGA AGAGAAGATC 
ATTATTGCTG AGGTTGTGAA CAAAATTAAT CGGGCTAATG GGAAGAGTAC ATCTCGGATT 
TTCCTCTTAA CAAACAATAA CCTTCTCCTT GCTGACCAAA AGTCTGGGCA GATCAAGTCC 
GAGGTCCCCC TGGTAGATGT GACCAAGGTT TCCATGAGTT CCCAGAACGA TGGTTTCTTT 
GCAGTGCACC TCAAGGAGGG TTCAGAAGCG GCTAGTAAAG GGGACTTTCT CTTCAGCAGT 
GACCACCTGA TTGAAATGGC AACCAAGCTG TATCGCACGA CTCTCAGCCA AACTAAACAG 
AAGCTCAACA TCGAGATTTC CGATGAGTTC CTGGTACAGT TCAGACAGGA CAAAGTATGT 
GTGAAGTTTA TTCAAGGCAA CCAGAAAAAT GGGAGCGTGC CAACCTGCAA ACGAAAGAAC 
AACAGACTCC TTGAAGTCGC TGTCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495767.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495830.1 Mus musculus myosin IB (Myo1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu27296
Clone ID Related Accession (Same CDS sequence)	XM_006495767.3 , XM_006495767.2
Accession Version	XM_006495767.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGT ACGCAGGGAA 
TACAGGAAAT TCTTTAGAGC AAATGCTGGA AAGAAAATCT ATGAGTTTAC ACTTCAAAGA 
ATTGTGCAAA AATACTTGTT GGAAATGAAA AATAAAATGC CTTCCTTGTC ACCGATAGAT 
AAAAATTGGC CATCAAGACC TTACCTGTTC TTGGATTCGA CTCACAAGGA GCTAAAAAGG 
ATCTTCCACT TGTGGAGGTG TAAAAAATAC AGGGATCAAT TCACAGACCA GCAGAAACTT 
ATTTATGAAG AGAAGCTCGA GGCCAGCGAA CTCTTCAAAG ACAAGAAGGC TTTATATCCT 
TCTAGTGTTG GGCAACCATT CCAAGGAGCT TACCTGGAAA TCAACAAGAA CCCAAAATAT 
AAGAAACTCA AAGATGCCAT TGAAGAGAAG ATCATTATTG CTGAGGTTGT GAACAAAATT 
AATCGGGCTA ATGGGAAGAG TACATCTCGG ATTTTCCTCT TAACAAACAA TAACCTTCTC 
CTTGCTGACC AAAAGTCTGG GCAGATCAAG TCCGAGGTCC CCCTGGTAGA TGTGACCAAG 
GTTTCCATGA GTTCCCAGAA CGATGGTTTC TTTGCAGTGC ACCTCAAGGA GGGTTCAGAA 
GCGGCTAGTA AAGGGGACTT TCTCTTCAGC AGTGACCACC TGATTGAAAT GGCAACCAAG 
CTGTATCGCA CGACTCTCAG CCAAACTAAA CAGAAGCTCA ACATCGAGAT TTCCGATGAG 
TTCCTGGTAC AGTTCAGACA GGACAAAGTA TGTGTGAAGT TTATTCAAGG CAACCAGAAA 
AATGGGAGCG TGCCAACCTG CAAACGAAAG AACAACAGAC TCCTTGAAGT CGCTGTCCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495830.1
CDS	223..3465
Translation	MAKMEVKSSLLDNMIGVGDMVLLEPLNEETFIDNLKKRFDHNEIYTYIGSVVISVNPYRSLPIYSPEKVEDYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495767.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495767.2

ORF Insert Sequence:

ATGGCCAAAA TGGAGGTGAA ATCCTCACTT CTGGACAATA TGATCGGTGT TGGAGATATG 
GTTCTTTTAG AGCCGCTCAA TGAGGAGACG TTCATCGACA ACCTGAAGAA GCGCTTCGAC 
CACAATGAGA TATATACATA CATTGGGAGC GTGGTTATAT CTGTTAACCC CTACCGGTCC 
TTGCCCATAT ATTCGCCGGA GAAAGTGGAA GATTACAGAA ATAGAAACTT CTATGAACTG 
AGCCCTCACA TCTTTGCTTT GTCGGATGAA GCATACAGAT CCCTCCGTGA TCAAGATAAA 
GATCAATGCA TTCTCATTAC TGGGGAGAGT GGAGCAGGAA AAACAGAGGC CAGCAAGCTC 
GTCATGTCCT ATGTCGCTGC CGTGTGCGGA AAAGGAGCAG AAGTCAATCA GGTCAAGGAA 
CAGCTTCTGC AGTCCAACCC TGTCCTGGAA GCTTTTGGAA ATGCCAAGAC TGTGAGGAAT 
GACAACTCAT CTAGATTCGG CAAATACATG GATATTGAAT TTGACTTTAA AGGCGATCCG 
CTGGGAGGAG TGATAAGTAA CTATCTTTTA GAGAAATCTC GTGTTGTTAA GCAGCCAAGA 
GGTGAAAGAA ACTTCCATGT GTTCTATCAG CTGCTCTCAG GCGCCTCTGA GGAGCTCCTC 
TACAAGCTTA AGCTGGAGCG GGATTTCAGC AGATACAACT ATCTGAGTCT GGACTCTGCT 
AAAGTTAATG GTGTGGATGA TGCTGCCAAT TTCAGAACTG TTAGGAATGC GATGCAGATC 
GTGGGCTTTC TGGATCATGA GGCCGAGGCT GTCCTGGAGG TAGTGGCAGC CGTTTTGAAA 
CTGGGGAACA TCGAGTTCAA GCCTGAATCA CGAGTGAATG GTTTGGATGA AAGCAAAATC 
AAAGATAAAA ATGAGTTAAA AGAAATTTGC GAGTTGACCA GCATCGATCA GGTGGTTTTA 
GAAAGAGCGT TCAGTTTCCG AACAGTTGAA GCGAAGCAGG AGAAAGTTTC AACCACGCTG 
AATGTGGCTC AGGCTTATTA TGCCCGTGAT GCCCTGGCTA AAAACCTCTA CAGCCGCTTG 
TTTTCATGGT TGGTAAATCG TATCAACGAA AGCATTAAGG CACAAACAAA AGTGAGAAAG 
AAGGTCATGG GTGTTCTGGA CATTTATGGC TTTGAAATAT TTGAGAATGC AGATAACAGC 
TTCGAGCAGT TTATTATTAA CTATTGTAAT GAAAAGCTTC AACAAATCTT CATCGAACTT 
ACCCTCAAAG AAGAGCAAGA GGAATATATT CGGGAGGACA TAGAATGGAC TCACATTGAC 
TACTTCAACA ATGCTATCAT TTGTGACCTA ATAGAAAATA ACACAAATGG AATCTTGGCC 
ATGCTGGATG AAGAGTGCCT GAGACCCGGC ACTGTTACAG ATGAGACCTT CCTGGAAAAG 
CTGAACCAAG TCTGTGCCAC CCACCAGCAC TTTGAGAGCA GGATGAGCAA GTGTTCCCGC 
TTTCTCAACG ACACAACCCT GCCCCACAGC TGCTTCAGAA TCCAGCATTA TGCTGGCAAG 
GTGCTCTACC AGGTGGAAGG ATTTGTTGAC AAGAACAATG ACCTTCTGTA CCGGGACCTG 
TCTCAAGCCA TGTGGAAGGC CGGCCACTCC CTCATCAAGT CTCTGTTTCC TGAAGGAAAT 
CCTGCAAAGG TCAACCTGAA GAGACCTCCC ACCGCAGGCT CCCAGTTCAA GGCGTCTGTG 
GCCACGCTGA TGAGAAACCT GCAGACCAAG AACCCGAACT ACATCAGGTG CATCAAGCCA 
AATGATAAAA AGGCAGCTCA CATTTTCAAT GAGAGTCTCG TATGCCATCA GATCAGGTAC 
CTGGGTCTTT TGGAGAACGT TCGAGTGAGA CGGGCAGGCT ATGCCTTCAG GCAGGCCTAT 
GAACCTTGCC TGGAAAGATA CAAGATGCTT TGCAAACAAA CGTGGCCTCA CTGGAAGGGA 
CCAGCAAGGT CTGGCGTGGA GGTTCTGTTT AATGAGCTAG AGATTCCTGT GGAAGAGCAC 
TCCTTCGGGA GATCCAAGAT ATTCATCCGA AACCCACGGA CATTATTCCA ACTAGAAGAC 
CTAAGGAAGC AGCGGCTGGA GGACCTGGCC ACTCTCATTC AGAAGATTTA TCGAGGATGG 
AAATGCCGTA CACATTTTCT GCTCATGAAA AGAAGCCAAG TTGTGATTGC TGCCTGGTAC 
AGGAGATATG CGCAACAAAA GCGGTACCAG CAGATAAAGA GTTCAGCCCT GGTGATTCAG 
TCGTACATCC GGGGCTGGAA GGCTCGAAAA ATCCTGCGGG AGCTGAAGCA TCAGAAGCGC 
TGTAAGGAGG CAGCCACCAC CATCGCGGCG TACTGGCATG GCACCCAGGT ACGCAGGGAA 
TACAGGAAAT TCTTTAGAGC AAATGCTGGA AAGAAAATCT ATGAGTTTAC ACTTCAAAGA 
ATTGTGCAAA AATACTTGTT GGAAATGAAA AATAAAATGC CTTCCTTGTC ACCGATAGAT 
AAAAATTGGC CATCAAGACC TTACCTGTTC TTGGATTCGA CTCACAAGGA GCTAAAAAGG 
ATCTTCCACT TGTGGAGGTG TAAAAAATAC AGGGATCAAT TCACAGACCA GCAGAAACTT 
ATTTATGAAG AGAAGCTCGA GGCCAGCGAA CTCTTCAAAG ACAAGAAGGC TTTATATCCT 
TCTAGTGTTG GGCAACCATT CCAAGGAGCT TACCTGGAAA TCAACAAGAA CCCAAAATAT 
AAGAAACTCA AAGATGCCAT TGAAGAGAAG ATCATTATTG CTGAGGTTGT GAACAAAATT 
AATCGGGCTA ATGGGAAGAG TACATCTCGG ATTTTCCTCT TAACAAACAA TAACCTTCTC 
CTTGCTGACC AAAAGTCTGG GCAGATCAAG TCCGAGGTCC CCCTGGTAGA TGTGACCAAG 
GTTTCCATGA GTTCCCAGAA CGATGGTTTC TTTGCAGTGC ACCTCAAGGA GGGTTCAGAA 
GCGGCTAGTA AAGGGGACTT TCTCTTCAGC AGTGACCACC TGATTGAAAT GGCAACCAAG 
CTGTATCGCA CGACTCTCAG CCAAACTAAA CAGAAGCTCA ACATCGAGAT TTCCGATGAG 
TTCCTGGTAC AGTTCAGACA GGACAAAGTA TGTGTGAAGT TTATTCAAGG CAACCAGAAA 
AATGGGAGCG TGCCAACCTG CAAACGAAAG AACAACAGAC TCCTTGAAGT CGCTGTCCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1b ( XM_006495769.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495832.1 Mus musculus myosin IB (Myo1b), transcript variant X6, mRNA.

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CloneID	OMu27297
Clone ID Related Accession (Same CDS sequence)	XM_006495769.3
Accession Version	XM_006495769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ib isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTATG TCGCTGCCGT GTGCGGAAAA GGAGCAGAAG TCAATCAGGT CAAGGAACAG 
CTTCTGCAGT CCAACCCTGT CCTGGAAGCT TTTGGAAATG CCAAGACTGT GAGGAATGAC 
AACTCATCTA GATTCGGCAA ATACATGGAT ATTGAATTTG ACTTTAAAGG CGATCCGCTG 
GGAGGAGTGA TAAGTAACTA TCTTTTAGAG AAATCTCGTG TTGTTAAGCA GCCAAGAGGT 
GAAAGAAACT TCCATGTGTT CTATCAGCTG CTCTCAGGCG CCTCTGAGGA GCTCCTCTAC 
AAGCTTAAGC TGGAGCGGGA TTTCAGCAGA TACAACTATC TGAGTCTGGA CTCTGCTAAA 
GTTAATGGTG TGGATGATGC TGCCAATTTC AGAACTGTTA GGAATGCGAT GCAGATCGTG 
GGCTTTCTGG ATCATGAGGC CGAGGCTGTC CTGGAGGTAG TGGCAGCCGT TTTGAAACTG 
GGGAACATCG AGTTCAAGCC TGAATCACGA GTGAATGGTT TGGATGAAAG CAAAATCAAA 
GATAAAAATG AGTTAAAAGA AATTTGCGAG TTGACCAGCA TCGATCAGGT GGTTTTAGAA 
AGAGCGTTCA GTTTCCGAAC AGTTGAAGCG AAGCAGGAGA AAGTTTCAAC CACGCTGAAT 
GTGGCTCAGG CTTATTATGC CCGTGATGCC CTGGCTAAAA ACCTCTACAG CCGCTTGTTT 
TCATGGTTGG TAAATCGTAT CAACGAAAGC ATTAAGGCAC AAACAAAAGT GAGAAAGAAG 
GTCATGGGTG TTCTGGACAT TTATGGCTTT GAAATATTTG AGAATGCAGA TAACAGCTTC 
GAGCAGTTTA TTATTAACTA TTGTAATGAA AAGCTTCAAC AAATCTTCAT CGAACTTACC 
CTCAAAGAAG AGCAAGAGGA ATATATTCGG GAGGACATAG AATGGACTCA CATTGACTAC 
TTCAACAATG CTATCATTTG TGACCTAATA GAAAATAACA CAAATGGAAT CTTGGCCATG 
CTGGATGAAG AGTGCCTGAG ACCCGGCACT GTTACAGATG AGACCTTCCT GGAAAAGCTG 
AACCAAGTCT GTGCCACCCA CCAGCACTTT GAGAGCAGGA TGAGCAAGTG TTCCCGCTTT 
CTCAACGACA CAACCCTGCC CCACAGCTGC TTCAGAATCC AGCATTATGC TGGCAAGGTG 
CTCTACCAGG TGGAAGGATT TGTTGACAAG AACAATGACC TTCTGTACCG GGACCTGTCT 
CAAGCCATGT GGAAGGCCGG CCACTCCCTC ATCAAGTCTC TGTTTCCTGA AGGAAATCCT 
GCAAAGGTCA ACCTGAAGAG ACCTCCCACC GCAGGCTCCC AGTTCAAGGC GTCTGTGGCC 
ACGCTGATGA GAAACCTGCA GACCAAGAAC CCGAACTACA TCAGGTGCAT CAAGCCAAAT 
GATAAAAAGG CAGCTCACAT TTTCAATGAG AGTCTCGTAT GCCATCAGAT CAGGTACCTG 
GGTCTTTTGG AGAACGTTCG AGTGAGACGG GCAGGCTATG CCTTCAGGCA GGCCTATGAA 
CCTTGCCTGG AAAGATACAA GATGCTTTGC AAACAAACGT GGCCTCACTG GAAGGGACCA 
GCAAGGTCTG GCGTGGAGGT TCTGTTTAAT GAGCTAGAGA TTCCTGTGGA AGAGCACTCC 
TTCGGGAGAT CCAAGATATT CATCCGAAAC CCACGGACAT TATTCCAACT AGAAGACCTA 
AGGAAGCAGC GGCTGGAGGA CCTGGCCACT CTCATTCAGA AGATTTATCG AGGATGGAAA 
TGCCGTACAC ATTTTCTGCT CATGAAAAGA AGCCAAGTTG TGATTGCTGC CTGGTACAGG 
AGATATGCGC AACAAAAGCG GTACCAGCAG ATAAAGAGTT CAGCCCTGGT GATTCAGTCG 
TACATCCGGG GCTGGAAGGC TCGAAAAATC CTGCGGGAGC TGAAGCATCA GAAGCGCTGT 
AAGGAGGCAG CCACCACCAT CGCGGCGTAC TGGCATGGCA CCCAGGCGCG AAGGGAGCTG 
AGACGGCTGA AGGACGAGGC TAGGAATAAG CACGCTATTG CAGTTATTTG GGCTTACTGG 
CTTGGATCGA AGGCACGGAG GGAGTTGAAA CGCTTGAAGG AGGAGGCTAG GCGTAAGCAT 
GCAGTTGCTG TCATTTGGGC TTACTGGCTT GGACTGAAGG TACGCAGGGA ATACAGGAAA 
TTCTTTAGAG CAAATGCTGG AAAGAAAATC TATGAGTTTA CACTTCAAAG AATTGTGCAA 
AAATACTTGT TGGAAATGAA AAATAAAATG CCTTCCTTGT CACCGATAGA TAAAAATTGG 
CCATCAAGAC CTTACCTGTT CTTGGATTCG ACTCACAAGG AGCTAAAAAG GATCTTCCAC 
TTGTGGAGGT GTAAAAAATA CAGGGATCAA TTCACAGACC AGCAGAAACT TATTTATGAA 
GAGAAGCTCG AGGCCAGCGA ACTCTTCAAA GACAAGAAGG CTTTATATCC TTCTAGTGTT 
GGGCAACCAT TCCAAGGAGC TTACCTGGAA ATCAACAAGA ACCCAAAATA TAAGAAACTC 
AAAGATGCCA TTGAAGAGAA GATCATTATT GCTGAGGTTG TGAACAAAAT TAATCGGGCT 
AATGGGAAGA GTACATCTCG GATTTTCCTC TTAACAAACA ATAACCTTCT CCTTGCTGAC 
CAAAAGTCTG GGCAGATCAA GTCCGAGGTC CCCCTGGTAG ATGTGACCAA GGTTTCCATG 
AGTTCCCAGA ACGATGGTTT CTTTGCAGTG CACCTCAAGG AGGGTTCAGA AGCGGCTAGT 
AAAGGGGACT TTCTCTTCAG CAGTGACCAC CTGATTGAAA TGGCAACCAA GCTGTATCGC 
ACGACTCTCA GCCAAACTAA ACAGAAGCTC AACATCGAGA TTTCCGATGA GTTCCTGGTA 
CAGTTCAGAC AGGACAAAGT ATGTGTGAAG TTTATTCAAG GCAACCAGAA AAATGGGAGC 
GTGCCAACCT GCAAACGAAA GAACAACAGA CTCCTTGAAG TCGCTGTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495832.1
CDS	507..3560
Translation	MSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLYKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFLDHEAEAVLEVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTSIDQVVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFENADNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTTLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKAGHSLIKSLFPEGNPAKVNLKRPPTAGSQFKASVATLMRNLQTKNPNYIRCIKPNDKKAAHIFNESLVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEHSFGRSKIFIRNPRTLFQLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKRSQVVIAAWYRRYAQQKRYQQIKSSALVIQSYIRGWKARKILRELKHQKRCKEAATTIAAYWHGTQARRELRRLKDEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYLLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP

Target ORF information:

RefSeq Version	XM_006495769.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IB (Myo1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495769.3

ORF Insert Sequence:

ATGTCCTATG TCGCTGCCGT GTGCGGAAAA GGAGCAGAAG TCAATCAGGT CAAGGAACAG 
CTTCTGCAGT CCAACCCTGT CCTGGAAGCT TTTGGAAATG CCAAGACTGT GAGGAATGAC 
AACTCATCTA GATTCGGCAA ATACATGGAT ATTGAATTTG ACTTTAAAGG CGATCCGCTG 
GGAGGAGTGA TAAGTAACTA TCTTTTAGAG AAATCTCGTG TTGTTAAGCA GCCAAGAGGT 
GAAAGAAACT TCCATGTGTT CTATCAGCTG CTCTCAGGCG CCTCTGAGGA GCTCCTCTAC 
AAGCTTAAGC TGGAGCGGGA TTTCAGCAGA TACAACTATC TGAGTCTGGA CTCTGCTAAA 
GTTAATGGTG TGGATGATGC TGCCAATTTC AGAACTGTTA GGAATGCGAT GCAGATCGTG 
GGCTTTCTGG ATCATGAGGC CGAGGCTGTC CTGGAGGTAG TGGCAGCCGT TTTGAAACTG 
GGGAACATCG AGTTCAAGCC TGAATCACGA GTGAATGGTT TGGATGAAAG CAAAATCAAA 
GATAAAAATG AGTTAAAAGA AATTTGCGAG TTGACCAGCA TCGATCAGGT GGTTTTAGAA 
AGAGCGTTCA GTTTCCGAAC AGTTGAAGCG AAGCAGGAGA AAGTTTCAAC CACGCTGAAT 
GTGGCTCAGG CTTATTATGC CCGTGATGCC CTGGCTAAAA ACCTCTACAG CCGCTTGTTT 
TCATGGTTGG TAAATCGTAT CAACGAAAGC ATTAAGGCAC AAACAAAAGT GAGAAAGAAG 
GTCATGGGTG TTCTGGACAT TTATGGCTTT GAAATATTTG AGAATGCAGA TAACAGCTTC 
GAGCAGTTTA TTATTAACTA TTGTAATGAA AAGCTTCAAC AAATCTTCAT CGAACTTACC 
CTCAAAGAAG AGCAAGAGGA ATATATTCGG GAGGACATAG AATGGACTCA CATTGACTAC 
TTCAACAATG CTATCATTTG TGACCTAATA GAAAATAACA CAAATGGAAT CTTGGCCATG 
CTGGATGAAG AGTGCCTGAG ACCCGGCACT GTTACAGATG AGACCTTCCT GGAAAAGCTG 
AACCAAGTCT GTGCCACCCA CCAGCACTTT GAGAGCAGGA TGAGCAAGTG TTCCCGCTTT 
CTCAACGACA CAACCCTGCC CCACAGCTGC TTCAGAATCC AGCATTATGC TGGCAAGGTG 
CTCTACCAGG TGGAAGGATT TGTTGACAAG AACAATGACC TTCTGTACCG GGACCTGTCT 
CAAGCCATGT GGAAGGCCGG CCACTCCCTC ATCAAGTCTC TGTTTCCTGA AGGAAATCCT 
GCAAAGGTCA ACCTGAAGAG ACCTCCCACC GCAGGCTCCC AGTTCAAGGC GTCTGTGGCC 
ACGCTGATGA GAAACCTGCA GACCAAGAAC CCGAACTACA TCAGGTGCAT CAAGCCAAAT 
GATAAAAAGG CAGCTCACAT TTTCAATGAG AGTCTCGTAT GCCATCAGAT CAGGTACCTG 
GGTCTTTTGG AGAACGTTCG AGTGAGACGG GCAGGCTATG CCTTCAGGCA GGCCTATGAA 
CCTTGCCTGG AAAGATACAA GATGCTTTGC AAACAAACGT GGCCTCACTG GAAGGGACCA 
GCAAGGTCTG GCGTGGAGGT TCTGTTTAAT GAGCTAGAGA TTCCTGTGGA AGAGCACTCC 
TTCGGGAGAT CCAAGATATT CATCCGAAAC CCACGGACAT TATTCCAACT AGAAGACCTA 
AGGAAGCAGC GGCTGGAGGA CCTGGCCACT CTCATTCAGA AGATTTATCG AGGATGGAAA 
TGCCGTACAC ATTTTCTGCT CATGAAAAGA AGCCAAGTTG TGATTGCTGC CTGGTACAGG 
AGATATGCGC AACAAAAGCG GTACCAGCAG ATAAAGAGTT CAGCCCTGGT GATTCAGTCG 
TACATCCGGG GCTGGAAGGC TCGAAAAATC CTGCGGGAGC TGAAGCATCA GAAGCGCTGT 
AAGGAGGCAG CCACCACCAT CGCGGCGTAC TGGCATGGCA CCCAGGCGCG AAGGGAGCTG 
AGACGGCTGA AGGACGAGGC TAGGAATAAG CACGCTATTG CAGTTATTTG GGCTTACTGG 
CTTGGATCGA AGGCACGGAG GGAGTTGAAA CGCTTGAAGG AGGAGGCTAG GCGTAAGCAT 
GCAGTTGCTG TCATTTGGGC TTACTGGCTT GGACTGAAGG TACGCAGGGA ATACAGGAAA 
TTCTTTAGAG CAAATGCTGG AAAGAAAATC TATGAGTTTA CACTTCAAAG AATTGTGCAA 
AAATACTTGT TGGAAATGAA AAATAAAATG CCTTCCTTGT CACCGATAGA TAAAAATTGG 
CCATCAAGAC CTTACCTGTT CTTGGATTCG ACTCACAAGG AGCTAAAAAG GATCTTCCAC 
TTGTGGAGGT GTAAAAAATA CAGGGATCAA TTCACAGACC AGCAGAAACT TATTTATGAA 
GAGAAGCTCG AGGCCAGCGA ACTCTTCAAA GACAAGAAGG CTTTATATCC TTCTAGTGTT 
GGGCAACCAT TCCAAGGAGC TTACCTGGAA ATCAACAAGA ACCCAAAATA TAAGAAACTC 
AAAGATGCCA TTGAAGAGAA GATCATTATT GCTGAGGTTG TGAACAAAAT TAATCGGGCT 
AATGGGAAGA GTACATCTCG GATTTTCCTC TTAACAAACA ATAACCTTCT CCTTGCTGAC 
CAAAAGTCTG GGCAGATCAA GTCCGAGGTC CCCCTGGTAG ATGTGACCAA GGTTTCCATG 
AGTTCCCAGA ACGATGGTTT CTTTGCAGTG CACCTCAAGG AGGGTTCAGA AGCGGCTAGT 
AAAGGGGACT TTCTCTTCAG CAGTGACCAC CTGATTGAAA TGGCAACCAA GCTGTATCGC 
ACGACTCTCA GCCAAACTAA ACAGAAGCTC AACATCGAGA TTTCCGATGA GTTCCTGGTA 
CAGTTCAGAC AGGACAAAGT ATGTGTGAAG TTTATTCAAG GCAACCAGAA AAATGGGAGC 
GTGCCAACCT GCAAACGAAA GAACAACAGA CTCCTTGAAG TCGCTGTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Myo1b
Entrez Gene ID	17912
Full Name	myosin IB
Synonyms	AA406997,MIH-L,MMIa
General protein information	Preferred Names myosin IB Names unconventional myosin-Ib MMI-alpha Myosin IA (Myosin I alpha) (MMI-alpha) (MMIa) (MIH-L) molecular motor myosin I alpha myosin-Ib
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C1.1\|1 26.58 cM

Canis lupus familiaris (dog)	MYO1B	XP_005640529.1
Mus musculus (house mouse)	Myo1b	NP_001155289.1
Gallus gallus (chicken)	MYO1B	XP_004942631.1
Danio rerio (zebrafish)	myo1b	XP_005168006.1
Drosophila melanogaster (fruit fly)	Myo95E	NP_001027208.1
Rattus norvegicus (Norway rat)	Myo1b	NP_446438.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004403	XP_313688.5
Homo sapiens (human)	MYO1B	NP_001155291.1
	LOC694510	XP_002808062.1
Pan troglodytes (chimpanzee)	MYO1B	XP_003309419.1
Bos taurus (cattle)	MYO1B	NP_001095669.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Myo1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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