Mylpf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mylpf
Entrez Gene ID	17907
Full Name	myosin light chain, phosphorylatable, fast skeletal muscle
Synonyms	2410014J02Rik,MLC-2,Mlc2
General protein information	Preferred Names myosin light chain, phosphorylatable, fast skeletal muscle Names myosin regulatory light chain 2, skeletal muscle isoform MLC2F fast skeletal myosin light chain 2 myosin light chain 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006507421.2	XP_006507484.1	myosin regulatory light chain 2, skeletal muscle isoform isoform X1
NM_001347655.1	NP_001334584.1	myosin regulatory light chain 2, skeletal muscle isoform isoform 2
NM_016754.5	NP_058034.1	myosin regulatory light chain 2, skeletal muscle isoform isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYLPF	NP_037424.2
Xenopus tropicalis (tropical clawed frog)	mylpf	NP_989158.1
Danio rerio (zebrafish)	mylpfa	NP_571263.1
Canis lupus familiaris (dog)	MYLPF	XP_536899.2
Bos taurus (cattle)	MYLPF	NP_001069115.1
Mus musculus (house mouse)	Mylpf	NP_058034.1
Rattus norvegicus (Norway rat)	Mylpf	NP_036737.1
Danio rerio (zebrafish)	mylpfb	NP_001004668.1
Caenorhabditis elegans (roundworm)	mlc-2	NP_510828.1
Caenorhabditis elegans (roundworm)	mlc-1	NP_510829.2

Gene Ontology
Bibliography

Related articles in PubMed

ADAMTS3 activity is mandatory for embryonic lymphangiogenesis and regulates placental angiogenesis.
Janssen L, Dupont L, Bekhouche M, Noel A, Leduc C, Voz M, Peers B, Cataldo D, Apte SS, Dubail J, Colige A
Angiogenesis19(1)53-65(2016 Jan)

Deletion of Mylk1 in oocytes causes delayed morula-to-blastocyst transition and reduced fertility without affecting folliculogenesis and oocyte maturation in mice.
Liang QX, Zhang QH, Qi ST, Wang ZW, Hu MW, Ma XS, Zhu MS, Schatten H, Wang ZB, Sun QY
Biology of reproduction92(4)97(2015 Apr)

The FgfrL1 receptor is required for development of slow muscle fibers.
Amann R, Wyder S, Slavotinek AM, Trueb B
Developmental biology394(2)228-41(2014 Oct)

Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice.
Harms MJ, Ishibashi J, Wang W, Lim HW, Goyama S, Sato T, Kurokawa M, Won KJ, Seale P
Cell metabolism19(4)593-604(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicated that sine oculis homeobox 1 (Six1) overexpression could significantly promote the expression of fast-type muscle genes Atp2a1, Srl, and Mylpf.
Title: Overexpression of Six1 gene suppresses proliferation and enhances expression of fast-type muscle genes in C2C12 myoblasts.

Data suggest that the overexpression of fast skeletal muscle myosin light chain in murine asthma may be associated with the changes of bronchial smooth muscle.
Title: The fast skeletal muscle myosin light chain is differentially expressed in smooth muscle cells of OVA-challenged mouse trachea.

RLC-f is necessary for skeletal muscle formation. The lack of skeletal muscle formation observed in RLC-f null mice indicates the total dependence of skeletal muscle development on the presence of RLC-f during embryogenesis.
Title: Fast skeletal muscle regulatory light chain is required for fast and slow skeletal muscle development.

Therefore, we suggest that Chr 21 gene(s) distal from the ETS2 locus reduce mouse mlc2a and PEBP expression in DS model mice and DS.
Title: Human chromosome 21q22.2-qter carries a gene(s) responsible for downregulation of mlc2a and PEBP in Down syndrome model mice.

The following Mylpf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mylpf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu39352	NM_001347655.1 Latest version!	Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu39352	XM_006507421.2 Latest version!	Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu10666	NM_016754.5 Latest version!	Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mylpf ( NM_001347655.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334584.1 Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu39352
Clone ID Related Accession (Same CDS sequence)	XM_006507421.2 , NM_001347655.1
Accession Version	NM_001347655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	myosin regulatory light chain 2, skeletal muscle isoform isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122537.4. On Dec 3, 2016 this sequence version replaced XM_006507422.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AA538095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGACC AGACTCAGAT CCAGGAGTTC AAGGAGGCGT TCACTGTAAT TGATCAGAAC 
AGGGATGGCA TTATCGACAA AGAGGATCTT CGGGACACCT TTGCAGCCAT GGGCCGTCTC 
AATGTGAAGA ATGAGGAACT CGACGCTATG ATGAAGGAAG CCAGTGGGCC CATCAACTTC 
ACTGTCTTCC TGACCATGTT TGGGGAAAAG CTTAAGGGTG CGGACCCGGA GGATGTGATC 
ACTGGAGCCT TCAAGGTCCT GGACCCAGAA GGGAAGGGCA CCATCAAAAA GCAGTTCTTG 
GAGGAGCTGC TTACCACGCA GTGCGACCGA TTTTCCCAGG AGGAGATCAA GAACATGTGG 
GCAGCCTTCC CCCCAGACGT GGGCGGCAAC GTGGACTACA AGAACATCTG CTACGTCATC 
ACACATGGTG ATGCTAAGGA CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334584.1
CDS	111..560
Translation	MFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKQFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_001347655.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347655.1

ORF Insert Sequence:

ATGTTTGACC AGACTCAGAT CCAGGAGTTC AAGGAGGCGT TCACTGTAAT TGATCAGAAC 
AGGGATGGCA TTATCGACAA AGAGGATCTT CGGGACACCT TTGCAGCCAT GGGCCGTCTC 
AATGTGAAGA ATGAGGAACT CGACGCTATG ATGAAGGAAG CCAGTGGGCC CATCAACTTC 
ACTGTCTTCC TGACCATGTT TGGGGAAAAG CTTAAGGGTG CGGACCCGGA GGATGTGATC 
ACTGGAGCCT TCAAGGTCCT GGACCCAGAA GGGAAGGGCA CCATCAAAAA GCAGTTCTTG 
GAGGAGCTGC TTACCACGCA GTGCGACCGA TTTTCCCAGG AGGAGATCAA GAACATGTGG 
GCAGCCTTCC CCCCAGACGT GGGCGGCAAC GTGGACTACA AGAACATCTG CTACGTCATC 
ACACATGGTG ATGCTAAGGA CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylpf ( XM_006507421.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507484.1 Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39352
Clone ID Related Accession (Same CDS sequence)	XM_006507421.2 , NM_001347655.1
Accession Version	XM_006507421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin regulatory light chain 2, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACC AGACTCAGAT CCAGGAGTTC AAGGAGGCGT TCACTGTAAT TGATCAGAAC 
AGGGATGGCA TTATCGACAA AGAGGATCTT CGGGACACCT TTGCAGCCAT GGGCCGTCTC 
AATGTGAAGA ATGAGGAACT CGACGCTATG ATGAAGGAAG CCAGTGGGCC CATCAACTTC 
ACTGTCTTCC TGACCATGTT TGGGGAAAAG CTTAAGGGTG CGGACCCGGA GGATGTGATC 
ACTGGAGCCT TCAAGGTCCT GGACCCAGAA GGGAAGGGCA CCATCAAAAA GCAGTTCTTG 
GAGGAGCTGC TTACCACGCA GTGCGACCGA TTTTCCCAGG AGGAGATCAA GAACATGTGG 
GCAGCCTTCC CCCCAGACGT GGGCGGCAAC GTGGACTACA AGAACATCTG CTACGTCATC 
ACACATGGTG ATGCTAAGGA CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507484.1
CDS	277..726
Translation	MFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKQFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	XM_006507421.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507421.2

ORF Insert Sequence:

ATGTTTGACC AGACTCAGAT CCAGGAGTTC AAGGAGGCGT TCACTGTAAT TGATCAGAAC 
AGGGATGGCA TTATCGACAA AGAGGATCTT CGGGACACCT TTGCAGCCAT GGGCCGTCTC 
AATGTGAAGA ATGAGGAACT CGACGCTATG ATGAAGGAAG CCAGTGGGCC CATCAACTTC 
ACTGTCTTCC TGACCATGTT TGGGGAAAAG CTTAAGGGTG CGGACCCGGA GGATGTGATC 
ACTGGAGCCT TCAAGGTCCT GGACCCAGAA GGGAAGGGCA CCATCAAAAA GCAGTTCTTG 
GAGGAGCTGC TTACCACGCA GTGCGACCGA TTTTCCCAGG AGGAGATCAA GAACATGTGG 
GCAGCCTTCC CCCCAGACGT GGGCGGCAAC GTGGACTACA AGAACATCTG CTACGTCATC 
ACACATGGTG ATGCTAAGGA CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mylpf ( NM_016754.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058034.1 Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10666
Clone ID Related Accession (Same CDS sequence)	NM_016754.5
Accession Version	NM_016754.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	myosin regulatory light chain 2, skeletal muscle isoform isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122537.4. On Apr 28, 2007 this sequence version replaced NM_016754.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK010483.1, BY709905.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAAGGCCAA GAGAAGGGCA GGAGCGGAAG GGAGCTCCAA CGTCTTCTCC 
ATGTTTGACC AGACTCAGAT CCAGGAGTTC AAGGAGGCGT TCACTGTAAT TGATCAGAAC 
AGGGATGGCA TTATCGACAA AGAGGATCTT CGGGACACCT TTGCAGCCAT GGGCCGTCTC 
AATGTGAAGA ATGAGGAACT CGACGCTATG ATGAAGGAAG CCAGTGGGCC CATCAACTTC 
ACTGTCTTCC TGACCATGTT TGGGGAAAAG CTTAAGGGTG CGGACCCGGA GGATGTGATC 
ACTGGAGCCT TCAAGGTCCT GGACCCAGAA GGGAAGGGCA CCATCAAAAA GCAGTTCTTG 
GAGGAGCTGC TTACCACGCA GTGCGACCGA TTTTCCCAGG AGGAGATCAA GAACATGTGG 
GCAGCCTTCC CCCCAGACGT GGGCGGCAAC GTGGACTACA AGAACATCTG CTACGTCATC 
ACACATGGTG ATGCTAAGGA CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058034.1
CDS	154..663
Translation	MAPKKAKRRAGAEGSSNVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKQFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_016754.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016754.5

ORF Insert Sequence:

ATGGCACCCA AGAAGGCCAA GAGAAGGGCA GGAGCGGAAG GGAGCTCCAA CGTCTTCTCC 
ATGTTTGACC AGACTCAGAT CCAGGAGTTC AAGGAGGCGT TCACTGTAAT TGATCAGAAC 
AGGGATGGCA TTATCGACAA AGAGGATCTT CGGGACACCT TTGCAGCCAT GGGCCGTCTC 
AATGTGAAGA ATGAGGAACT CGACGCTATG ATGAAGGAAG CCAGTGGGCC CATCAACTTC 
ACTGTCTTCC TGACCATGTT TGGGGAAAAG CTTAAGGGTG CGGACCCGGA GGATGTGATC 
ACTGGAGCCT TCAAGGTCCT GGACCCAGAA GGGAAGGGCA CCATCAAAAA GCAGTTCTTG 
GAGGAGCTGC TTACCACGCA GTGCGACCGA TTTTCCCAGG AGGAGATCAA GAACATGTGG 
GCAGCCTTCC CCCCAGACGT GGGCGGCAAC GTGGACTACA AGAACATCTG CTACGTCATC 
ACACATGGTG ATGCTAAGGA CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ADAMTS3 activity is mandatory for embryonic lymphangiogenesis and regulates placental angiogenesis.
Angiogenesis19(1)53-65(2016 Jan)
Janssen L,Dupont L,Bekhouche M,Noel A,Leduc C,Voz M,Peers B,Cataldo D,Apte SS,Dubail J,Colige A

Deletion of Mylk1 in oocytes causes delayed morula-to-blastocyst transition and reduced fertility without affecting folliculogenesis and oocyte maturation in mice.
Biology of reproduction92(4)97(2015 Apr)
Liang QX,Zhang QH,Qi ST,Wang ZW,Hu MW,Ma XS,Zhu MS,Schatten H,Wang ZB,Sun QY

The FgfrL1 receptor is required for development of slow muscle fibers.
Developmental biology394(2)228-41(2014 Oct)
Amann R,Wyder S,Slavotinek AM,Trueb B

Prdm16 is required for the maintenance of brown adipocyte identity and function in adult mice.
Cell metabolism19(4)593-604(2014 Apr)
Harms MJ,Ishibashi J,Wang W,Lim HW,Goyama S,Sato T,Kurokawa M,Won KJ,Seale P

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Mylpf cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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