Laptm4a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Laptm4a
Entrez Gene ID	17775
Full Name	lysosomal-associated protein transmembrane 4A
Synonyms	AA286466,LAPTM4,MTP,Mtrp,mKIAA0108
General protein information	Preferred Names lysosomal-associated protein transmembrane 4A Names lysosomal-associated transmembrane protein 4A golgi 4-transmembrane-spanning transporter membrane transporter protein transporter protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_008640.2	NP_032666.2	lysosomal-associated transmembrane protein 4A

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LAPTM4A	NP_001026263.1
Drosophila melanogaster (fruit fly)	CG14767	NP_652400.3
Pan troglodytes (chimpanzee)	LAPTM4A	XP_003949812.1
	LAPTM4A	XP_001095082.1
Mus musculus (house mouse)	Laptm4a	NP_032666.2
Bos taurus (cattle)	LAPTM4A	NP_991368.1
Homo sapiens (human)	LAPTM4A	NP_055528.1
Canis lupus familiaris (dog)	LAPTM4A	XP_003639626.1
Rattus norvegicus (Norway rat)	Laptm4a	NP_955416.1
Danio rerio (zebrafish)	laptm4a	NP_999850.2
Xenopus tropicalis (tropical clawed frog)	laptm4a	NP_001004797.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.
Milkereit R, Rotin D
PloS one6(11)e27478(2011)

Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
Mortensen M, Ferguson DJ, Edelmann M, Kessler B, Morten KJ, Komatsu M, Simon AK
Proceedings of the National Academy of Sciences of the United States of America107(2)832-7(2010 Jan)

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Tkatchenko TV, Moreno-Rodriguez RA, Conway SJ, Molkentin JD, Markwald RR, Tkatchenko AV
Physiological genomics39(3)160-8(2009 Nov)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that while some requisites of LAPTM5 lysosomal sorting are conserved among LAPTM4 proteins, LAPTM4a and LAPTM4b have also developed distinct sorting requirements.
Title: A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.

LAPTM4 alpha is the first example of a membrane protein that requires two tandemly arranged tyrosine-based sorting signals for efficient localization in lysosomes and endosomes.
Title: Lysosome-associated protein transmembrane 4 alpha (LAPTM4 alpha) requires two tandemly arranged tyrosine-based signals for sorting to lysosomes.

The following Laptm4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Laptm4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02434	NM_008640.2 Latest version!	Mus musculus lysosomal-associated protein transmembrane 4A (Laptm4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Laptm4a ( NM_008640.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032666.2 Mus musculus lysosomal-associated protein transmembrane 4A (Laptm4a), mRNA.

Price & Availability↑

CloneID	OMu02434
Clone ID Related Accession (Same CDS sequence)	NM_008640.2
Accession Version	NM_008640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	lysosomal-associated transmembrane protein 4A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW495200.1, AK084515.1, BY785283.1 and BC086667.1. On Jun 28, 2006 this sequence version replaced NM_008640.1. ##Evidence-Data-START## Transcript exon combination :: BC086667.1, AK084515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCAGA AGGCGGAGGG AGAGATTCCG AGCCGACGTC CCGCGAGAGC GGCGCCGGGC 
GCCCAGAGCG CAGGCGCACA GGAGTTGGCA AGGGCCCGGG CCGAGGGCGG GCCAGAAGAC 
GCGTCCTTTG GCTGTGGACA CCGTCGTAGA GTCGTCTGGG TTGAACCGCT GGGCCGAAAG 
CTTTTGTCAG GGGCCTGGGC GAGAACGAGG CCCGCGAGGT TCCGGATGGT GTCCATGACT 
TTCAAACGGA GCCGCAGCGA CCGGTTCTAC AGCACCCGGT GCTGCGGCTG TTGCCATGTC 
CGCACCGGGA CGATCATCCT GGGGACCTGG TACATGGTGG TCAACCTGTT GATGGCCATT 
TTGCTGACCG TGGAGGTGAC TCATCCAAAT TCCATGCCAG CTGTCAACAT TCAGTATGAA 
GTCATTGGTA ATTACTATTC ATCTGAGAGA ATGGCTGATA ATGCCTGCGT TCTTTTTGCC 
GTCTCTGTTC TTATGTTTAT AATCAGTTCA ATGCTGGTTT ATGGAGCCAT TTCTTATCAA 
GTGGGCTGGC TGATTCCTTT CTTCTGCTAC CGACTCTTTG ACTTCGTTCT CAGTTGTCTG 
GTGGCCATCA GTTCTCTCAC TTACTTGCCA AGAATCAAAG AATATCTGGA TCAGTTGCCT 
GATTTCCCAT ACAAAGATGA CCTCCTGGCA TTGGACTCCA GCTGCCTCCT GTTCATCGTC 
CTTGTGTTCT TTGTGGTATT CATCATTTTT AAGGCTTACC TAATCAATTG TGTTTGGAAC 
TGCTACAAAT ACATCAACAA CAGAAATGTG CCAGAGATTG CCGTGTACCC TGCCTTTGAA 
ACACCTCCAC AGTATGTTTT GCCAACCTAT GAAATGGCTG TGAAGATTCC TGAGAAAGAG 
CCACCACCTC CTTACCTGCC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032666.2
CDS	653..1579
Translation	MWQKAEGEIPSRRPARAAPGAQSAGAQELARARAEGGPEDASFGCGHRRRVVWVEPLGRKLLSGAWARTRPARFRMVSMTFKRSRSDRFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNYYSSERMADNACVLFAVSVLMFIISSMLVYGAISYQVGWLIPFFCYRLFDFVLSCLVAISSLTYLPRIKEYLDQLPDFPYKDDLLALDSSCLLFIVLVFFVVFIIFKAYLINCVWNCYKYINNRNVPEIAVYPAFETPPQYVLPTYEMAVKIPEKEPPPPYLPA

Target ORF information:

RefSeq Version	NM_008640.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysosomal-associated protein transmembrane 4A (Laptm4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008640.2

ORF Insert Sequence:

ATGTGGCAGA AGGCGGAGGG AGAGATTCCG AGCCGACGTC CCGCGAGAGC GGCGCCGGGC 
GCCCAGAGCG CAGGCGCACA GGAGTTGGCA AGGGCCCGGG CCGAGGGCGG GCCAGAAGAC 
GCGTCCTTTG GCTGTGGACA CCGTCGTAGA GTCGTCTGGG TTGAACCGCT GGGCCGAAAG 
CTTTTGTCAG GGGCCTGGGC GAGAACGAGG CCCGCGAGGT TCCGGATGGT GTCCATGACT 
TTCAAACGGA GCCGCAGCGA CCGGTTCTAC AGCACCCGGT GCTGCGGCTG TTGCCATGTC 
CGCACCGGGA CGATCATCCT GGGGACCTGG TACATGGTGG TCAACCTGTT GATGGCCATT 
TTGCTGACCG TGGAGGTGAC TCATCCAAAT TCCATGCCAG CTGTCAACAT TCAGTATGAA 
GTCATTGGTA ATTACTATTC ATCTGAGAGA ATGGCTGATA ATGCCTGCGT TCTTTTTGCC 
GTCTCTGTTC TTATGTTTAT AATCAGTTCA ATGCTGGTTT ATGGAGCCAT TTCTTATCAA 
GTGGGCTGGC TGATTCCTTT CTTCTGCTAC CGACTCTTTG ACTTCGTTCT CAGTTGTCTG 
GTGGCCATCA GTTCTCTCAC TTACTTGCCA AGAATCAAAG AATATCTGGA TCAGTTGCCT 
GATTTCCCAT ACAAAGATGA CCTCCTGGCA TTGGACTCCA GCTGCCTCCT GTTCATCGTC 
CTTGTGTTCT TTGTGGTATT CATCATTTTT AAGGCTTACC TAATCAATTG TGTTTGGAAC 
TGCTACAAAT ACATCAACAA CAGAAATGTG CCAGAGATTG CCGTGTACCC TGCCTTTGAA 
ACACCTCCAC AGTATGTTTT GCCAACCTAT GAAATGGCTG TGAAGATTCC TGAGAAAGAG 
CCACCACCTC CTTACCTGCC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.
PloS one6(11)e27478(2011)
Milkereit R,Rotin D

Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo.
Proceedings of the National Academy of Sciences of the United States of America107(2)832-7(2010 Jan)
Mortensen M,Ferguson DJ,Edelmann M,Kessler B,Morten KJ,Komatsu M,Simon AK

Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease.
Physiological genomics39(3)160-8(2009 Nov)
Tkatchenko TV,Moreno-Rodriguez RA,Conway SJ,Molkentin JD,Markwald RR,Tkatchenko AV

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

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Laptm4a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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