Mras cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mras
Entrez Gene ID	17532
Full Name	muscle and microspikes RAS
Synonyms	2900078C09Rik,AI326250
General protein information	Preferred Names muscle and microspikes RAS Names ras-related protein M-Ras X-Ras ras-related protein R-Ras3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030244081.1	XP_030099941.1	ras-related protein M-Ras isoform X2
XM_030244082.1	XP_030099942.1	ras-related protein M-Ras isoform X3
XM_006510825.2	XP_006510888.1	ras-related protein M-Ras isoform X1
NM_008624.3	NP_032650.1	ras-related protein M-Ras precursor
XM_006510825.3	XP_006510888.1	ras-related protein M-Ras isoform X1
XM_030244080.1	XP_030099940.1	ras-related protein M-Ras isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mras	NP_037113.2
Bos taurus (cattle)	MRAS	NP_001091587.1
Mus musculus (house mouse)	Mras	NP_032650.1
Danio rerio (zebrafish)	LOC100536317	XP_005167818.1
Caenorhabditis elegans (roundworm)	ras-2	NP_497972.1
Xenopus tropicalis (tropical clawed frog)	LOC100496446	XP_002943203.1
Homo sapiens (human)	MRAS	NP_036351.3
Canis lupus familiaris (dog)	MRAS	XP_534277.2
Pan troglodytes (chimpanzee)	MRAS	XP_003310061.1
	MRAS	XP_001114261.1
Gallus gallus (chicken)	MRAS	NP_989820.1

Gene Ontology
Bibliography

Related articles in PubMed

Transient recruitment of M-Ras GTPase to phagocytic cups in RAW264 macrophages during Fc?R-mediated phagocytosis.
Egami Y, Araki N
Microscopy (Oxford, England)(2018 Jan)

Urinary Retention, Incontinence, and Dysregulation of Muscarinic Receptors in Male Mice Lacking Mras.
Ehrhardt A, Wang B, Yung AC, Wang Y, Kozlowski P, van Breemen C, Schrader JW
PloS one10(10)e0141493(2015)

Genetic dissection of plexin signaling in vivo.
Worzfeld T, Swiercz JM, Sent?rk A, Genz B, Korostylev A, Deng S, Xia J, Hoshino M, Epstein JA, Chan AM, Vollmar B, Acker-Palmer A, Kuner R, Offermanns S
Proceedings of the National Academy of Sciences of the United States of America111(6)2194-9(2014 Feb)

MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell plasticity and Xenopus embryonic cell fate.
Mathieu ME, Faucheux C, Saucourt C, Soulet F, Gauthereau X, F?dou S, Trouillas M, Th?z? N, Thi?baud P, Boeuf H
Development (Cambridge, England)140(16)3311-22(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of the muscarinic M2 and M3 receptors that mediate the cholinergic contractile stimuli of the detrusor muscle was dysregulated in both Mras-/- males and females, although only males exhibited a urinary phenotype.
Title: Urinary Retention, Incontinence, and Dysregulation of Muscarinic Receptors in Male Mice Lacking Mras.

Overexpression of MRAS in mouse embryonic stem cells leads to different cellular phenotypes.
Title: MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell plasticity and Xenopus embryonic cell fate.

succeed in solving two crystal structures corresponding to state 1 and state 2 from a single Ras polypeptide, M-RasD41E, carrying an H-Ras-type substitution in residue 41
Title: Critical roles of interactions among switch I-preceding residues and between switch II and its neighboring alpha-helix in conformational dynamics of the GTP-bound Ras family small GTPases.

X-ray crystal structure analyses of a series of mutant H-Ras and M-Ras in complex with guanosine 5'-(beta,gamma-imido)triphosphate (GppNHp), representing various intermediate states of the transition, were determined.
Title: Structural basis for conformational dynamics of GTP-bound Ras protein.

These results imply that M-Ras, induced and activated by BMP-2 signaling, participates in the osteoblastic determination, differentiation, and transdifferentiation under p38 MAPK and JNK regulation
Title: M-Ras is activated by bone morphogenetic protein-2 and participates in osteoblastic determination, differentiation, and transdifferentiation.

The following Mras gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mras cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21927	NM_008624.3 Latest version!	Mus musculus muscle and microspikes RAS (Mras), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu21927	XM_030244080.1 Latest version!	Mus musculus muscle and microspikes RAS (Mras), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu21927	XM_006510825.3 Latest version!	Mus musculus muscle and microspikes RAS (Mras), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu90070	XM_030244081.1 Latest version!	Mus musculus muscle and microspikes RAS (Mras), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu90071	XM_030244082.1 Latest version!	Mus musculus muscle and microspikes RAS (Mras), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu21927	XM_006510825.2	Mus musculus muscle and microspikes RAS (Mras), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mras ( NM_008624.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032650.1 Mus musculus muscle and microspikes RAS (Mras), mRNA.

Price & Availability↑

CloneID	OMu21927
Clone ID Related Accession (Same CDS sequence)	XM_006510825.2 , NM_008624.3 , XM_006510825.3 , XM_030244080.1
Accession Version	NM_008624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	ras-related protein M-Ras precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX122916.1, CJ162751.1, AB004879.1, AK050469.1, AC157994.2 and BC023079.1. On Apr 17, 2007 this sequence version replaced NM_008624.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.238883.1, SRR1660817.221420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849390, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032650.1
CDS	421..1047
Translation	MATSAVPSENLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLNVDKTFHDLVRVIRQQVPEKNQKKKKKTKWRGDRATGTHKLQCVIL

Target ORF information:

RefSeq Version	NM_008624.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus muscle and microspikes RAS (Mras), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008624.3

ORF Insert Sequence:

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mras ( XM_006510825.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510888.1 Mus musculus muscle and microspikes RAS (Mras), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21927
Clone ID Related Accession (Same CDS sequence)	XM_006510825.2 , NM_008624.3 , XM_006510825.3 , XM_030244080.1
Accession Version	XM_006510825.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ras-related protein M-Ras isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510888.1
CDS	102..728
Translation	MATSAVPSENLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLNVDKTFHDLVRVIRQQVPEKNQKKKKKTKWRGDRATGTHKLQCVIL

Target ORF information:

RefSeq Version	XM_006510825.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus muscle and microspikes RAS (Mras), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510825.2

ORF Insert Sequence:

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mras ( XM_006510825.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510888.1 Mus musculus muscle and microspikes RAS (Mras), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21927
Clone ID Related Accession (Same CDS sequence)	XM_006510825.2 , NM_008624.3 , XM_006510825.3 , XM_030244080.1
Accession Version	XM_006510825.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras-related protein M-Ras isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510888.1
CDS	838..1464
Translation	MATSAVPSENLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLNVDKTFHDLVRVIRQQVPEKNQKKKKKTKWRGDRATGTHKLQCVIL

Target ORF information:

RefSeq Version	XM_006510825.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus muscle and microspikes RAS (Mras), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510825.3

ORF Insert Sequence:

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mras ( XM_030244080.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099940.1 Mus musculus muscle and microspikes RAS (Mras), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu21927
Clone ID Related Accession (Same CDS sequence)	XM_006510825.2 , NM_008624.3 , XM_006510825.3 , XM_030244080.1
Accession Version	XM_030244080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras-related protein M-Ras isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099940.1
CDS	258..884
Translation	MATSAVPSENLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLNVDKTFHDLVRVIRQQVPEKNQKKKKKTKWRGDRATGTHKLQCVIL

Target ORF information:

RefSeq Version	XM_030244080.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus muscle and microspikes RAS (Mras), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244080.1

ORF Insert Sequence:

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGCA ACAGGTTCCA 
GAGAAAAACC AGAAGAAGAA AAAGAAGACA AAATGGCGAG GAGACAGGGC CACCGGCACT 
CACAAACTGC AGTGTGTCAT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mras ( XM_030244081.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099941.1 Mus musculus muscle and microspikes RAS (Mras), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu90070
Clone ID Related Accession (Same CDS sequence)	XM_030244081.1
Accession Version	XM_030244081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras-related protein M-Ras isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099941.1
CDS	448..978
Translation	MATSAVPSENLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLNVDKTFHDLVRVIR

Target ORF information:

RefSeq Version	XM_030244081.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus muscle and microspikes RAS (Mras), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244081.1

ORF Insert Sequence:

ATGGCGACCA GCGCTGTTCC AAGTGAAAAC CTTCCCACAT ATAAACTAGT AGTGGTGGGA 
GATGGTGGTG TGGGCAAGAG TGCGCTCACT ATTCAGTTTT TCCAGAAGAT CTTTGTGCCT 
GACTACGACC CCACCATTGA AGACTCCTAC CTGAAGCATA CAGAGATTGA CAATCAGTGG 
GCCATCTTGG ATGTTCTGGA CACAGCCGGG CAGGAGGAGT TCAGTGCCAT GCGGGAACAA 
TACATGCGCA CAGGGGATGG CTTCCTCATT GTCTACTCCG TCACCGACAA GGCCAGCTTC 
GAGCACGTGG ACCGCTTCCA CCAGCTCATT CTGCGTGTCA AGGACAGGGA GTCATTCCCA 
ATGATCCTCG TGGCCAACAA GGTGGATCTG ATGCACCTAA GGAAAGTCAC CAGGGACCAA 
GGAAAAGAAA TGGCAACCAA ATACAATATC CCATATATAG AGACCAGTGC CAAGGACCCG 
CCTCTCAACG TGGATAAAAC CTTCCATGAC CTAGTTAGAG TAATTAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mras ( XM_030244082.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099942.1 Mus musculus muscle and microspikes RAS (Mras), transcript variant X4, mRNA.

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CloneID	OMu90071
Clone ID Related Accession (Same CDS sequence)	XM_030244082.1
Accession Version	XM_030244082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras-related protein M-Ras isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGAAC AATACATGCG CACAGGGGAT GGCTTCCTCA TTGTCTACTC CGTCACCGAC 
AAGGCCAGCT TCGAGCACGT GGACCGCTTC CACCAGCTCA TTCTGCGTGT CAAGGACAGG 
GAGTCATTCC CAATGATCCT CGTGGCCAAC AAGGTGGATC TGATGCACCT AAGGAAAGTC 
ACCAGGGACC AAGGAAAAGA AATGGCAACC AAATACAATA TCCCATATAT AGAGACCAGT 
GCCAAGGACC CGCCTCTCAA CGTGGATAAA ACCTTCCATG ACCTAGTTAG AGTAATTAGG 
CAACAGGTTC CAGAGAAAAA CCAGAAGAAG AAAAAGAAGA CAAAATGGCG AGGAGACAGG 
GCCACCGGCA CTCACAAACT GCAGTGTGTC ATCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099942.1
CDS	117..515
Translation	MREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMILVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLNVDKTFHDLVRVIRQQVPEKNQKKKKKTKWRGDRATGTHKLQCVIL

Target ORF information:

RefSeq Version	XM_030244082.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus muscle and microspikes RAS (Mras), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244082.1

ORF Insert Sequence:

ATGCGGGAAC AATACATGCG CACAGGGGAT GGCTTCCTCA TTGTCTACTC CGTCACCGAC 
AAGGCCAGCT TCGAGCACGT GGACCGCTTC CACCAGCTCA TTCTGCGTGT CAAGGACAGG 
GAGTCATTCC CAATGATCCT CGTGGCCAAC AAGGTGGATC TGATGCACCT AAGGAAAGTC 
ACCAGGGACC AAGGAAAAGA AATGGCAACC AAATACAATA TCCCATATAT AGAGACCAGT 
GCCAAGGACC CGCCTCTCAA CGTGGATAAA ACCTTCCATG ACCTAGTTAG AGTAATTAGG 
CAACAGGTTC CAGAGAAAAA CCAGAAGAAG AAAAAGAAGA CAAAATGGCG AGGAGACAGG 
GCCACCGGCA CTCACAAACT GCAGTGTGTC ATCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transient recruitment of M-Ras GTPase to phagocytic cups in RAW264 macrophages during Fc?R-mediated phagocytosis.
Microscopy (Oxford, England)(2018 Jan)
Egami Y,Araki N

Urinary Retention, Incontinence, and Dysregulation of Muscarinic Receptors in Male Mice Lacking Mras.
PloS one10(10)e0141493(2015)
Ehrhardt A,Wang B,Yung AC,Wang Y,Kozlowski P,van Breemen C,Schrader JW

Genetic dissection of plexin signaling in vivo.
Proceedings of the National Academy of Sciences of the United States of America111(6)2194-9(2014 Feb)
Worzfeld T,Swiercz JM,Sent?rk A,Genz B,Korostylev A,Deng S,Xia J,Hoshino M,Epstein JA,Chan AM,Vollmar B,Acker-Palmer A,Kuner R,Offermanns S

MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell plasticity and Xenopus embryonic cell fate.
Development (Cambridge, England)140(16)3311-22(2013 Aug)
Mathieu ME,Faucheux C,Saucourt C,Soulet F,Gauthereau X,F?dou S,Trouillas M,Th?z? N,Thi?baud P,Boeuf H

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Mras cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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