ORF » Species Summary » Mus musculus » Me1 cDNA ORF clone

Me1 cDNA ORF clone, Mus musculus(house mouse)

The following Me1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Me1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu13060 NM_008615.2
Latest version!		 Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu13293 NM_001198933.1
Latest version!		 Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu68208 XM_011242666.2
Latest version!		 Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Me1 ( NM_008615.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032641.2 Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OMu13060
    Clone ID Related Accession (Same CDS sequence) NM_008615.2
    Accession Version NM_008615.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-03
    Organism Mus musculus(house mouse)
    Product NADP-dependent malic enzyme isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ070715.1, AK077968.1 and AV363687.1. On Dec 9, 2007 this sequence version replaced NM_008615.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077968.1, AK004980.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCCCC GAGCCCCCCG CCGCCGACAC ACCCACCAGC GCGGCTACCT GCTGACGCGG 
    GACCCGCATC TCAACAAGGA CTTGGCTTTT ACTCTGGAAG AGAGACAGCA GTTGAACATT 
    CATGGATTGT TGCCGCCCTG CATCATCAGC CAGGAGCTCC AGGTCCTTAG AATAATTAAG 
    AATTTCGAAC GACTGAACTC TGACTTCGAC AGGTATCTCC TGTTAATGGA CCTGCAAGAC 
    AGAAATGAGA AGCTCTTCTA CAGCGTGCTC ATGTCTGATG TTGAAAAGTT CATGCCTATT 
    GTTTACACCC CCACCGTGGG CCTCGCATGC CAGCAGTACA GTTTGGCATT CCGGAAGCCA 
    AGAGGCCTCT TTATTAGTAT CCATGACAAA GGGCACATTG CTTCAGTTCT TAATGCATGG 
    CCAGAGGATG TCGTCAAGGC TATTGTGGTA ACTGATGGAG AGCGCATCCT TGGCTTGGGA 
    GACCTTGGCT GTAATGGGAT GGGCATCCCT GTGGGTAAAC TGGCCCTGTA CACGGCATGT 
    GGAGGGGTGA ACCCACAACA GTGTCTACCC ATCACTTTGG ATGTGGGAAC AGAAAATGAG 
    GAGTTACTTA AGGATCCACT GTACATCGGG CTGCGGCACC GGCGAGTCAG AGGCCCTGAG 
    TATGACGCCT TCCTGGATGA GTTCATGGAG GCAGCGTCTT CCAAATATGG CATGAATTGC 
    CTTATTCAGT TTGAAGATTT TGCCAATCGG AATGCATTTC GTCTCCTGAA CAAGTATCGA 
    AACAAGTATT GCACATTTAA CGATGATATT CAAGGAACAG CGTCTGTTGC GGTTGCGGGT 
    CTCCTTGCAG CTCTTCGAAT AACCAAGAAC AAGCTCTCTG ATCAGACAGT GCTGTTCCAG 
    GGAGCTGGAG AGGCTGCCTT GGGGATTGCT CACTTGGTTG TTATGGCCAT GGAGAAAGAA 
    GGTTTATCAA AGGAGAATGC TAGAAAGAAG ATATGGTTGG TTGACTCAAA AGGACTAATA 
    GTTAAGGGTC GTGCATCTCT CACAGAAGAG AAAGAGGTGT TTGCCCATGA ACATGAAGAA 
    ATGAAGAATC TGGAAGCCAT TGTTCAAAAG ATAAAACCAA CTGCCCTCAT AGGAGTTGCT 
    GCAATTGGTG GTGCTTTCAC TGAACAAATT CTCAAGGATA TGGCTGCCTT CAACGAGCGG 
    CCCATCATCT TTGCTTTGAG TAATCCGACC AGCAAAGCGG AGTGCTCTGC AGAGCAGTGC 
    TACAAGGTGA CCAAGGGACG TGCAATCTTT GCCAGCGGCA GTCCTTTTGA TCCAGTCACT 
    CTCCCAGATG GACGGACTCT GTTTCCTGGC CAAGGCAACA ATTCCTACGT GTTCCCTGGA 
    GTTGCTCTTG GGGTGGTGGC CTGCGGACTG AGACACATCG ATGATAAGGT CTTCCTCACC 
    ACTGCTGAGG TCATATCTCA GCAAGTGTCA GATAAACACC TGCAAGAAGG CCGGCTCTAT 
    CCTCCTTTGA ATACCATTCG AGGCGTTTCG TTGAAAATTG CAGTAAAGAT TGTGCAAGAT 
    GCATACAAAG AAAAGATGGC CACTGTTTAT CCTGAACCCC AAAACAAAGA AGAATTTGTC 
    TCCTCCCAGA TGTACAGCAC TAATTATGAC CAGATCCTAC CTGATTGTTA TCCGTGGCCT 
    GCAGAAGTCC AGAAAATACA GACCAAAGTC AACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032641.2
    CDS165..1883
    Translation

    Target ORF information:

    RefSeq Version NM_008615.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_008615.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCCCC GAGCCCCCCG CCGCCGACAC ACCCACCAGC GCGGCTACCT GCTGACGCGG 
    GACCCGCATC TCAACAAGGA CTTGGCTTTT ACTCTGGAAG AGAGACAGCA GTTGAACATT 
    CATGGATTGT TGCCGCCCTG CATCATCAGC CAGGAGCTCC AGGTCCTTAG AATAATTAAG 
    AATTTCGAAC GACTGAACTC TGACTTCGAC AGGTATCTCC TGTTAATGGA CCTGCAAGAC 
    AGAAATGAGA AGCTCTTCTA CAGCGTGCTC ATGTCTGATG TTGAAAAGTT CATGCCTATT 
    GTTTACACCC CCACCGTGGG CCTCGCATGC CAGCAGTACA GTTTGGCATT CCGGAAGCCA 
    AGAGGCCTCT TTATTAGTAT CCATGACAAA GGGCACATTG CTTCAGTTCT TAATGCATGG 
    CCAGAGGATG TCGTCAAGGC TATTGTGGTA ACTGATGGAG AGCGCATCCT TGGCTTGGGA 
    GACCTTGGCT GTAATGGGAT GGGCATCCCT GTGGGTAAAC TGGCCCTGTA CACGGCATGT 
    GGAGGGGTGA ACCCACAACA GTGTCTACCC ATCACTTTGG ATGTGGGAAC AGAAAATGAG 
    GAGTTACTTA AGGATCCACT GTACATCGGG CTGCGGCACC GGCGAGTCAG AGGCCCTGAG 
    TATGACGCCT TCCTGGATGA GTTCATGGAG GCAGCGTCTT CCAAATATGG CATGAATTGC 
    CTTATTCAGT TTGAAGATTT TGCCAATCGG AATGCATTTC GTCTCCTGAA CAAGTATCGA 
    AACAAGTATT GCACATTTAA CGATGATATT CAAGGAACAG CGTCTGTTGC GGTTGCGGGT 
    CTCCTTGCAG CTCTTCGAAT AACCAAGAAC AAGCTCTCTG ATCAGACAGT GCTGTTCCAG 
    GGAGCTGGAG AGGCTGCCTT GGGGATTGCT CACTTGGTTG TTATGGCCAT GGAGAAAGAA 
    GGTTTATCAA AGGAGAATGC TAGAAAGAAG ATATGGTTGG TTGACTCAAA AGGACTAATA 
    GTTAAGGGTC GTGCATCTCT CACAGAAGAG AAAGAGGTGT TTGCCCATGA ACATGAAGAA 
    ATGAAGAATC TGGAAGCCAT TGTTCAAAAG ATAAAACCAA CTGCCCTCAT AGGAGTTGCT 
    GCAATTGGTG GTGCTTTCAC TGAACAAATT CTCAAGGATA TGGCTGCCTT CAACGAGCGG 
    CCCATCATCT TTGCTTTGAG TAATCCGACC AGCAAAGCGG AGTGCTCTGC AGAGCAGTGC 
    TACAAGGTGA CCAAGGGACG TGCAATCTTT GCCAGCGGCA GTCCTTTTGA TCCAGTCACT 
    CTCCCAGATG GACGGACTCT GTTTCCTGGC CAAGGCAACA ATTCCTACGT GTTCCCTGGA 
    GTTGCTCTTG GGGTGGTGGC CTGCGGACTG AGACACATCG ATGATAAGGT CTTCCTCACC 
    ACTGCTGAGG TCATATCTCA GCAAGTGTCA GATAAACACC TGCAAGAAGG CCGGCTCTAT 
    CCTCCTTTGA ATACCATTCG AGGCGTTTCG TTGAAAATTG CAGTAAAGAT TGTGCAAGAT 
    GCATACAAAG AAAAGATGGC CACTGTTTAT CCTGAACCCC AAAACAAAGA AGAATTTGTC 
    TCCTCCCAGA TGTACAGCAC TAATTATGAC CAGATCCTAC CTGATTGTTA TCCGTGGCCT 
    GCAGAAGTCC AGAAAATACA GACCAAAGTC AACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Me1 ( NM_001198933.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185862.1 Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OMu13293
    Clone ID Related Accession (Same CDS sequence) NM_001198933.1
    Accession Version NM_001198933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1659bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-03
    Organism Mus musculus(house mouse)
    Product NADP-dependent malic enzyme isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK163403.1, AK077968.1 and AV363687.1. Transcript Variant: This variant (2) has an alternate exon at its 5' UTR and 5' coding region, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK163403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGACTGA TCTGCGAAGA CTTGGCTTTT ACTCTGGAAG AGAGACAGCA GTTGAACATT 
    CATGGATTGT TGCCGCCCTG CATCATCAGC CAGGAGCTCC AGGTCCTTAG AATAATTAAG 
    AATTTCGAAC GACTGAACTC TGACTTCGAC AGGTATCTCC TGTTAATGGA CCTGCAAGAC 
    AGAAATGAGA AGCTCTTCTA CAGCGTGCTC ATGTCTGATG TTGAAAAGTT CATGCCTATT 
    GTTTACACCC CCACCGTGGG CCTCGCATGC CAGCAGTACA GTTTGGCATT CCGGAAGCCA 
    AGAGGCCTCT TTATTAGTAT CCATGACAAA GGGCACATTG CTTCAGTTCT TAATGCATGG 
    CCAGAGGATG TCGTCAAGGC TATTGTGGTA ACTGATGGAG AGCGCATCCT TGGCTTGGGA 
    GACCTTGGCT GTAATGGGAT GGGCATCCCT GTGGGTAAAC TGGCCCTGTA CACGGCATGT 
    GGAGGGGTGA ACCCACAACA GTGTCTACCC ATCACTTTGG ATGTGGGAAC AGAAAATGAG 
    GAGTTACTTA AGGATCCACT GTACATCGGG CTGCGGCACC GGCGAGTCAG AGGCCCTGAG 
    TATGACGCCT TCCTGGATGA GTTCATGGAG GCAGCGTCTT CCAAATATGG CATGAATTGC 
    CTTATTCAGT TTGAAGATTT TGCCAATCGG AATGCATTTC GTCTCCTGAA CAAGTATCGA 
    AACAAGTATT GCACATTTAA CGATGATATT CAAGGAACAG CGTCTGTTGC GGTTGCGGGT 
    CTCCTTGCAG CTCTTCGAAT AACCAAGAAC AAGCTCTCTG ATCAGACAGT GCTGTTCCAG 
    GGAGCTGGAG AGGCTGCCTT GGGGATTGCT CACTTGGTTG TTATGGCCAT GGAGAAAGAA 
    GGTTTATCAA AGGAGAATGC TAGAAAGAAG ATATGGTTGG TTGACTCAAA AGGACTAATA 
    GTTAAGGGTC GTGCATCTCT CACAGAAGAG AAAGAGGTGT TTGCCCATGA ACATGAAGAA 
    ATGAAGAATC TGGAAGCCAT TGTTCAAAAG ATAAAACCAA CTGCCCTCAT AGGAGTTGCT 
    GCAATTGGTG GTGCTTTCAC TGAACAAATT CTCAAGGATA TGGCTGCCTT CAACGAGCGG 
    CCCATCATCT TTGCTTTGAG TAATCCGACC AGCAAAGCGG AGTGCTCTGC AGAGCAGTGC 
    TACAAGGTGA CCAAGGGACG TGCAATCTTT GCCAGCGGCA GTCCTTTTGA TCCAGTCACT 
    CTCCCAGATG GACGGACTCT GTTTCCTGGC CAAGGCAACA ATTCCTACGT GTTCCCTGGA 
    GTTGCTCTTG GGGTGGTGGC CTGCGGACTG AGACACATCG ATGATAAGGT CTTCCTCACC 
    ACTGCTGAGG TCATATCTCA GCAAGTGTCA GATAAACACC TGCAAGAAGG CCGGCTCTAT 
    CCTCCTTTGA ATACCATTCG AGGCGTTTCG TTGAAAATTG CAGTAAAGAT TGTGCAAGAT 
    GCATACAAAG AAAAGATGGC CACTGTTTAT CCTGAACCCC AAAACAAAGA AGAATTTGTC 
    TCCTCCCAGA TGTACAGCAC TAATTATGAC CAGATCCTAC CTGATTGTTA TCCGTGGCCT 
    GCAGAAGTCC AGAAAATACA GACCAAAGTC AACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001185862.1
    CDS303..1961
    Translation

    Target ORF information:

    RefSeq Version NM_001198933.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001198933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGACTGA TCTGCGAAGA CTTGGCTTTT ACTCTGGAAG AGAGACAGCA GTTGAACATT 
    CATGGATTGT TGCCGCCCTG CATCATCAGC CAGGAGCTCC AGGTCCTTAG AATAATTAAG 
    AATTTCGAAC GACTGAACTC TGACTTCGAC AGGTATCTCC TGTTAATGGA CCTGCAAGAC 
    AGAAATGAGA AGCTCTTCTA CAGCGTGCTC ATGTCTGATG TTGAAAAGTT CATGCCTATT 
    GTTTACACCC CCACCGTGGG CCTCGCATGC CAGCAGTACA GTTTGGCATT CCGGAAGCCA 
    AGAGGCCTCT TTATTAGTAT CCATGACAAA GGGCACATTG CTTCAGTTCT TAATGCATGG 
    CCAGAGGATG TCGTCAAGGC TATTGTGGTA ACTGATGGAG AGCGCATCCT TGGCTTGGGA 
    GACCTTGGCT GTAATGGGAT GGGCATCCCT GTGGGTAAAC TGGCCCTGTA CACGGCATGT 
    GGAGGGGTGA ACCCACAACA GTGTCTACCC ATCACTTTGG ATGTGGGAAC AGAAAATGAG 
    GAGTTACTTA AGGATCCACT GTACATCGGG CTGCGGCACC GGCGAGTCAG AGGCCCTGAG 
    TATGACGCCT TCCTGGATGA GTTCATGGAG GCAGCGTCTT CCAAATATGG CATGAATTGC 
    CTTATTCAGT TTGAAGATTT TGCCAATCGG AATGCATTTC GTCTCCTGAA CAAGTATCGA 
    AACAAGTATT GCACATTTAA CGATGATATT CAAGGAACAG CGTCTGTTGC GGTTGCGGGT 
    CTCCTTGCAG CTCTTCGAAT AACCAAGAAC AAGCTCTCTG ATCAGACAGT GCTGTTCCAG 
    GGAGCTGGAG AGGCTGCCTT GGGGATTGCT CACTTGGTTG TTATGGCCAT GGAGAAAGAA 
    GGTTTATCAA AGGAGAATGC TAGAAAGAAG ATATGGTTGG TTGACTCAAA AGGACTAATA 
    GTTAAGGGTC GTGCATCTCT CACAGAAGAG AAAGAGGTGT TTGCCCATGA ACATGAAGAA 
    ATGAAGAATC TGGAAGCCAT TGTTCAAAAG ATAAAACCAA CTGCCCTCAT AGGAGTTGCT 
    GCAATTGGTG GTGCTTTCAC TGAACAAATT CTCAAGGATA TGGCTGCCTT CAACGAGCGG 
    CCCATCATCT TTGCTTTGAG TAATCCGACC AGCAAAGCGG AGTGCTCTGC AGAGCAGTGC 
    TACAAGGTGA CCAAGGGACG TGCAATCTTT GCCAGCGGCA GTCCTTTTGA TCCAGTCACT 
    CTCCCAGATG GACGGACTCT GTTTCCTGGC CAAGGCAACA ATTCCTACGT GTTCCCTGGA 
    GTTGCTCTTG GGGTGGTGGC CTGCGGACTG AGACACATCG ATGATAAGGT CTTCCTCACC 
    ACTGCTGAGG TCATATCTCA GCAAGTGTCA GATAAACACC TGCAAGAAGG CCGGCTCTAT 
    CCTCCTTTGA ATACCATTCG AGGCGTTTCG TTGAAAATTG CAGTAAAGAT TGTGCAAGAT 
    GCATACAAAG AAAAGATGGC CACTGTTTAT CCTGAACCCC AAAACAAAGA AGAATTTGTC 
    TCCTCCCAGA TGTACAGCAC TAATTATGAC CAGATCCTAC CTGATTGTTA TCCGTGGCCT 
    GCAGAAGTCC AGAAAATACA GACCAAAGTC AACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Me1 ( XM_011242666.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240968.1 Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu68208
    Clone ID Related Accession (Same CDS sequence) XM_011242666.2
    Accession Version XM_011242666.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NADP-dependent malic enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGACCTGC AAGACAGAAA TGAGAAGCTC TTCTACAGCG TGCTCATGTC TGATGTTGAA 
    AAGTTCATGC CTATTGTTTA CACCCCCACC GTGGGCCTCG CATGCCAGCA GTACAGTTTG 
    GCATTCCGGA AGCCAAGAGG CCTCTTTATT AGTATCCATG ACAAAGGGCA CATTGCTTCA 
    GTTCTTAATG CATGGCCAGA GGATGTCGTC AAGGCTATTG TGGTAACTGA TGGAGAGCGC 
    ATCCTTGGCT TGGGAGACCT TGGCTGTAAT GGGATGGGCA TCCCTGTGGG TAAACTGGCC 
    CTGTACACGG CATGTGGAGG GGTGAACCCA CAACAGTGTC TACCCATCAC TTTGGATGTG 
    GGAACAGAAA ATGAGGAGTT ACTTAAGGAT CCACTGTACA TCGGGCTGCG GCACCGGCGA 
    GTCAGAGGCC CTGAGTATGA CGCCTTCCTG GATGAGTTCA TGGAGGCAGC GTCTTCCAAA 
    TATGGCATGA ATTGCCTTAT TCAGTTTGAA GATTTTGCCA ATCGGAATGC ATTTCGTCTC 
    CTGAACAAGT ATCGAAACAA GTATTGCACA TTTAACGATG ATATTCAAGG AACAGCGTCT 
    GTTGCGGTTG CGGGTCTCCT TGCAGCTCTT CGAATAACCA AGAACAAGCT CTCTGATCAG 
    ACAGTGCTGT TCCAGGGAGC TGGAGAGGCT GCCTTGGGGA TTGCTCACTT GGTTGTTATG 
    GCCATGGAGA AAGAAGGTTT ATCAAAGGAG AATGCTAGAA AGAAGATATG GTTGGTTGAC 
    TCAAAAGGAC TAATAGTTAA GGGTCGTGCA TCTCTCACAG AAGAGAAAGA GGTGTTTGCC 
    CATGAACATG AAGAAATGAA GAATCTGGAA GCCATTGTTC AAAAGATAAA ACCAACTGCC 
    CTCATAGGAG TTGCTGCAAT TGGTGGTGCT TTCACTGAAC AAATTCTCAA GGATATGGCT 
    GCCTTCAACG AGCGGCCCAT CATCTTTGCT TTGAGTAATC CGACCAGCAA AGCGGAGTGC 
    TCTGCAGAGC AGTGCTACAA GGTGACCAAG GGACGTGCAA TCTTTGCCAG CGGCAGTCCT 
    TTTGATCCAG TCACTCTCCC AGATGGACGG ACTCTGTTTC CTGGCCAAGG CAACAATTCC 
    TACGTGTTCC CTGGAGTTGC TCTTGGGGTG GTGGCCTGCG GACTGAGACA CATCGATGAT 
    AAGGTCTTCC TCACCACTGC TGAGGTCATA TCTCAGCAAG TGTCAGATAA ACACCTGCAA 
    GAAGGCCGGC TCTATCCTCC TTTGAATACC ATTCGAGGCG TTTCGTTGAA AATTGCAGTA 
    AAGATTGTGC AAGATGCATA CAAAGAAAAG ATGGCCACTG TTTATCCTGA ACCCCAAAAC 
    AAAGAAGAAT TTGTCTCCTC CCAGATGTAC AGCACTAATT ATGACCAGAT CCTACCTGAT 
    TGTTATCCGT GGCCTGCAGA AGTCCAGAAA ATACAGACCA AAGTCAACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011240968.1
    CDS225..1718
    Translation

    Target ORF information:

    RefSeq Version XM_011242666.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011242666.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGACCTGC AAGACAGAAA TGAGAAGCTC TTCTACAGCG TGCTCATGTC TGATGTTGAA 
    AAGTTCATGC CTATTGTTTA CACCCCCACC GTGGGCCTCG CATGCCAGCA GTACAGTTTG 
    GCATTCCGGA AGCCAAGAGG CCTCTTTATT AGTATCCATG ACAAAGGGCA CATTGCTTCA 
    GTTCTTAATG CATGGCCAGA GGATGTCGTC AAGGCTATTG TGGTAACTGA TGGAGAGCGC 
    ATCCTTGGCT TGGGAGACCT TGGCTGTAAT GGGATGGGCA TCCCTGTGGG TAAACTGGCC 
    CTGTACACGG CATGTGGAGG GGTGAACCCA CAACAGTGTC TACCCATCAC TTTGGATGTG 
    GGAACAGAAA ATGAGGAGTT ACTTAAGGAT CCACTGTACA TCGGGCTGCG GCACCGGCGA 
    GTCAGAGGCC CTGAGTATGA CGCCTTCCTG GATGAGTTCA TGGAGGCAGC GTCTTCCAAA 
    TATGGCATGA ATTGCCTTAT TCAGTTTGAA GATTTTGCCA ATCGGAATGC ATTTCGTCTC 
    CTGAACAAGT ATCGAAACAA GTATTGCACA TTTAACGATG ATATTCAAGG AACAGCGTCT 
    GTTGCGGTTG CGGGTCTCCT TGCAGCTCTT CGAATAACCA AGAACAAGCT CTCTGATCAG 
    ACAGTGCTGT TCCAGGGAGC TGGAGAGGCT GCCTTGGGGA TTGCTCACTT GGTTGTTATG 
    GCCATGGAGA AAGAAGGTTT ATCAAAGGAG AATGCTAGAA AGAAGATATG GTTGGTTGAC 
    TCAAAAGGAC TAATAGTTAA GGGTCGTGCA TCTCTCACAG AAGAGAAAGA GGTGTTTGCC 
    CATGAACATG AAGAAATGAA GAATCTGGAA GCCATTGTTC AAAAGATAAA ACCAACTGCC 
    CTCATAGGAG TTGCTGCAAT TGGTGGTGCT TTCACTGAAC AAATTCTCAA GGATATGGCT 
    GCCTTCAACG AGCGGCCCAT CATCTTTGCT TTGAGTAATC CGACCAGCAA AGCGGAGTGC 
    TCTGCAGAGC AGTGCTACAA GGTGACCAAG GGACGTGCAA TCTTTGCCAG CGGCAGTCCT 
    TTTGATCCAG TCACTCTCCC AGATGGACGG ACTCTGTTTC CTGGCCAAGG CAACAATTCC 
    TACGTGTTCC CTGGAGTTGC TCTTGGGGTG GTGGCCTGCG GACTGAGACA CATCGATGAT 
    AAGGTCTTCC TCACCACTGC TGAGGTCATA TCTCAGCAAG TGTCAGATAA ACACCTGCAA 
    GAAGGCCGGC TCTATCCTCC TTTGAATACC ATTCGAGGCG TTTCGTTGAA AATTGCAGTA 
    AAGATTGTGC AAGATGCATA CAAAGAAAAG ATGGCCACTG TTTATCCTGA ACCCCAAAAC 
    AAAGAAGAAT TTGTCTCCTC CCAGATGTAC AGCACTAATT ATGACCAGAT CCTACCTGAT 
    TGTTATCCGT GGCCTGCAGA AGTCCAGAAA ATACAGACCA AAGTCAACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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