Mmp16 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mmp16
Entrez Gene ID	17389
Full Name	matrix metallopeptidase 16
Synonyms	MT-MMP 3,MT3-MMP,Mt3mmp
General protein information	Preferred Names matrix metallopeptidase 16 Names matrix metalloproteinase-16 Membrane type 3-MMP membrane-type matrix metalloproteinase 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 A3\|4 7.08 cM
Summary	This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein exhibit retarded growth of the skeleton, especially in the cranium and long bones. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320017.1	XP_017175506.1	matrix metalloproteinase-16 isoform X1
NM_019724.3	NP_062698.2	matrix metalloproteinase-16 precursor
XM_017320017.2	XP_017175506.1	matrix metalloproteinase-16 isoform X1
NM_019724.4	NP_062698.2	matrix metalloproteinase-16 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mmp16	NP_542954.1
Homo sapiens (human)	MMP16	NP_005932.2
Pan troglodytes (chimpanzee)	MMP16	XP_001136497.1
	MMP16	XP_001084206.1
Bos taurus (cattle)	MMP16	NP_001179551.1
Gallus gallus (chicken)	MMP16	NP_990528.1
Canis lupus familiaris (dog)	MMP16	XP_544165.3
Mus musculus (house mouse)	Mmp16	NP_062698.2
Danio rerio (zebrafish)	mmp16b	XP_700781.6
Xenopus tropicalis (tropical clawed frog)	mmp16	NP_001015992.1

Gene Ontology
Bibliography

Related articles in PubMed

MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding.
Sanz RL, Ferraro GB, Kacervosky J, Salesse C, Gowing E, Hua L, Rambaldi I, Beaubien F, Holmbeck K, Cloutier JF, L?vesque M, Murai K, Fournier AE
The Journal of neuroscience : the official journal of the Society for Neuroscience38(3)518-529(2018 Jan)

Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration.
Zhang WL, Chen YF, Meng HZ, Du JJ, Luan GN, Wang HQ, Yang MW, Luo ZJ
Journal of orthopaedic research : official publication of the Orthopaedic Research Society35(6)1323-1334(2017 06)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Membrane-type matrix metalloproteinases: Their functions and regulations.
Itoh Y
Matrix biology : journal of the International Society for Matrix Biology44-46207-23(2015 May-Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-155 contributed to the up-regulation of MMP-16, and MMP-16 further degraded aggrecan and collagen type II, leading to the dehydration and degeneration of intervertebral discs
Title: Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration.

Neuronal knockdown of MT3-MMP resulted in the accumulation of NgR1 at the cell surface and reduced the accumulation of the NgR1 cleavage fragment in medium conditioned by cortical neurons
Title: Membrane-type matrix metalloproteinase-3 regulates neuronal responsiveness to myelin through Nogo-66 receptor 1 cleavage.

Study demonstrates that MT3-MMP, is expressed in mesenchymal tissues of the skeleton and in peri-skeletal soft connective tissue; and MT3-MMP-deficient mice display growth inhibition tied to a decreased viability of mesenchymal cells in skeletal tissues.
Title: Membrane-type MMPs enable extracellular matrix permissiveness and mesenchymal cell proliferation during embryogenesis.

The following Mmp16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mmp16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11652	NM_019724.4 Latest version!	Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$356.30 $509.00
OMu11652	XM_017320017.2 Latest version!	Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu11652	XM_017320017.1	Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$356.30 $509.00
OMu11652	NM_019724.3	Mus musculus matrix metallopeptidase 16 (Mmp16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$356.30 $509.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mmp16 ( NM_019724.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062698.2 Mus musculus matrix metallopeptidase 16 (Mmp16), mRNA.

Price & Availability↑

CloneID	OMu11652
Clone ID Related Accession (Same CDS sequence)	XM_017320017.1 , NM_019724.3 , XM_017320017.2 , NM_019724.4
Accession Version	NM_019724.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	matrix metalloproteinase-16 precursor
Comment

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_062698.2

Translation

MILLAFSSGRRLDFVHRSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFNIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRRAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSVPPADPRRHDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGNGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITIWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRDKEGLSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV

Target ORF information:

RefSeq Version	NM_019724.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus matrix metallopeptidase 16 (Mmp16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019724.3

ORF Insert Sequence:

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmp16 ( NM_019724.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062698.2 Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11652
Clone ID Related Accession (Same CDS sequence)	XM_017320017.1 , NM_019724.3 , XM_017320017.2 , NM_019724.4
Accession Version	NM_019724.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	matrix metalloproteinase-16 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN460527.1, AK048543.1 and AL805957.12. On Feb 13, 2016 this sequence version replaced NM_019724.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK048543.1, SRR1660815.75365.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062698.2
CDS	264..2087
Translation	MILLAFSSGRRLDFVHRSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFNIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRRAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSVPPADPRRHDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGNGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITIWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRDKEGLSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV

Target ORF information:

RefSeq Version	NM_019724.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019724.4

ORF Insert Sequence:

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmp16 ( XM_017320017.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175506.1 Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu11652
Clone ID Related Accession (Same CDS sequence)	XM_017320017.1 , NM_019724.3 , XM_017320017.2 , NM_019724.4
Accession Version	XM_017320017.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	matrix metalloproteinase-16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175506.1
CDS	615..2438
Translation	MILLAFSSGRRLDFVHRSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFNIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRRAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSVPPADPRRHDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGNGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITIWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRDKEGLSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV

Target ORF information:

RefSeq Version	XM_017320017.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320017.1

ORF Insert Sequence:

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmp16 ( XM_017320017.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175506.1 Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant X1, mRNA.

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CloneID	OMu11652
Clone ID Related Accession (Same CDS sequence)	XM_017320017.1 , NM_019724.3 , XM_017320017.2 , NM_019724.4
Accession Version	XM_017320017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	matrix metalloproteinase-16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175506.1
CDS	604..2427
Translation	MILLAFSSGRRLDFVHRSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFNIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRRAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSVPPADPRRHDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGNGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITIWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRDKEGLSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV

Target ORF information:

RefSeq Version	XM_017320017.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus matrix metallopeptidase 16 (Mmp16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320017.2

ORF Insert Sequence:

ATGATCTTAC TCGCATTCAG CTCTGGAAGA AGGTTGGATT TCGTGCATCG TTCGGGTGTG 
TTTTTCTTGC AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT 
TTCAATGTGG AGGTTTGGTT ACAAAAATAC GGCTACCTTC CACCGACTGA CCCCAGAATG 
TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCAGCTCTAG CTGCCATGCA GCAGTTCTAT 
GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATCG ACTGGATGAA GAAGCCTCGA 
TGCGGTGTAC CAGACCAGAC AAGAGGCAGC TCTAAATTCA ACATTCGTCG AAAGCGCTAC 
GCATTAACTG GACAGAAATG GCAACACAAA CATATCACTT ACAGTATAAA GAACGTAACT 
CCAAAAGTTG GAGATCCTGA GACCCGGAGA GCAATTCGCC GTGCCTTTGA TGTGTGGCAG 
AATGTAACTC CTCTAACGTT TGAAGAAGTT CCCTACAGTG AACTAGAAAA CGGCAAACGT 
GATGTGGATA TAACCATCAT TTTTGCTTCT GGTTTTCATG GAGACAGTTC CCCATTTGAT 
GGGGAGGGAG GATTTTTGGC ACATGCTTAT TTCCCTGGAC CCGGAATTGG AGGCGACACC 
CATTTTGATT CTGATGAGCC ATGGACACTG GGAAATCCCA ATCATGATGG AAACGATTTA 
TTTCTTGTAG CAGTCCATGA GCTGGGACAT GCTCTAGGAC TGGAACATTC CAACGACCCC 
ACCGCTATCA TGGCCCCATT TTATCAGTAC ATGGAAACAG ACAACTTCAA GCTTCCTAAT 
GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATCCC TCCACCTACA 
AGACCTCTAC CGACAGTGCC CCCGCACCGA TCTGTTCCTC CAGCAGACCC AAGGAGACAT 
GACAGGCCCA AACCTCCTCG GCCTCCCACT GGCAGACCCT CCTATCCAGG AGCCAAACCC 
AACATCTGTG ATGGGAACTT CAACACTCTA GCTATTCTTC GAAGGGAGAT GTTTGTTTTC 
AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA 
ATTACTTACT TCTGGCGGGG CCTTCCTCCC AGTATTGATG CAGTTTATGA AAACAGTGAC 
GGAAATTTCG TCTTCTTTAA AGGCAACAAA TACTGGGTGT TCAAAGACAC AACTCTTCAA 
CCTGGTTACC CTCATGACTT GATTACCCTT GGGAATGGAA TCCCCCCTCA TGGTATTGAT 
TCTGCCATAT GGTGGGAAGA TGTTGGCAAA ACCTATTTCT TCAAAGGTGA CAGGTATTGG 
AGATATAGTG AAGAAATGAA AACCATGGAC CCTGGTTACC CCAAGCCCAT CACCATCTGG 
AAGGGGATCC CTGAATCACC TCAGGGAGCT TTCGTCCACA AAGAAAATGG CTTTACCTAT 
TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAATCAGA TACTCAAAGT AGAACCTGGT 
TATCCAAGAT CCATCCTCAA GGACTTTATG GGCTGTGATG GACCAACAGA CCGAGATAAA 
GAAGGACTCA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC 
ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG 
GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA 
CGCTCTATGC AAGAGTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding.
The Journal of neuroscience : the official journal of the Society for Neuroscience38(3)518-529(2018 Jan)
Sanz RL,Ferraro GB,Kacervosky J,Salesse C,Gowing E,Hua L,Rambaldi I,Beaubien F,Holmbeck K,Cloutier JF,L?vesque M,Murai K,Fournier AE

Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society35(6)1323-1334(2017 06)
Zhang WL,Chen YF,Meng HZ,Du JJ,Luan GN,Wang HQ,Yang MW,Luo ZJ

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Membrane-type matrix metalloproteinases: Their functions and regulations.
Matrix biology : journal of the International Society for Matrix Biology44-46207-23(2015 May-Jul)
Itoh Y

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Mmp16 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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