Trpm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpm1
Entrez Gene ID	17364
Full Name	transient receptor potential cation channel, subfamily M, member 1
Synonyms	4732499L03Rik,AI606771,Ltrpc1,Mlsn1
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 1 Names transient receptor potential cation channel subfamily M member 1 long transient receptor potential channel 1 melastatin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 C\|7 34.61 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017322013.1	XP_017177502.1	transient receptor potential cation channel subfamily M member 1 isoform X2
NM_001039104.2	NP_001034193.2	transient receptor potential cation channel subfamily M member 1 isoform 2
XM_017322012.2	XP_017177501.1	transient receptor potential cation channel subfamily M member 1 isoform X1
NM_018752.3	NP_061222.3	transient receptor potential cation channel subfamily M member 1 isoform 1
XM_017322012.1	XP_017177501.1	transient receptor potential cation channel subfamily M member 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPM1	NP_001238949.1
Pan troglodytes (chimpanzee)	TRPM1	XP_003318096.1
	TRPM1	XP_001116180.2
Xenopus tropicalis (tropical clawed frog)	trpm1	XP_002934962.2
Caenorhabditis elegans (roundworm)	gon-2	NP_001122507.1
Canis lupus familiaris (dog)	TRPM1	XP_005618325.1
Bos taurus (cattle)	TRPM1	XP_002696651.2
Rattus norvegicus (Norway rat)	Trpm1	NP_001032822.1
Mus musculus (house mouse)	Trpm1	NP_001034193.2
Gallus gallus (chicken)	TRPM1	XP_425066.3

Gene Ontology
Bibliography

Related articles in PubMed

The TRPM1 Channel Is Required for Development of the Rod ON Bipolar Cell-AII Amacrine Cell Pathway in the Retinal Circuit.
Kozuka T, Chaya T, Tamalu F, Shimada M, Fujimaki-Aoba K, Kuwahara R, Watanabe SI, Furukawa T
The Journal of neuroscience : the official journal of the Society for Neuroscience37(41)9889-9900(2017 Oct)

TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade.
Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T
Proceedings of the National Academy of Sciences of the United States of America107(1)332-7(2010 Jan)

Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10.
Rowan S, Chen CM, Young TL, Fisher DE, Cepko CL
Development (Cambridge, England)131(20)5139-52(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that TRPM1 channel opening is essential for rod bipolar pathway establishment in development
Title: The TRPM1 Channel Is Required for Development of the Rod ON Bipolar Cell-AII Amacrine Cell Pathway in the Retinal Circuit.

This study confirmed the localization of LRIT3 at the dendritic tips of depolarizing bipolar cells in mouse retina and demonstrated the dependence of TRPM1 localization on the presence of LRIT3.
Title: LRIT3 is essential to localize TRPM1 to the dendritic tips of depolarizing bipolar cells and may play a role in cone synapse formation.

results indicate that the serum of a patient with PR contained an antibody against TRPM1 caused an acute death of retinal ON bipolar cells of mice
Title: Degeneration of retinal on bipolar cells induced by serum including autoantibody against TRPM1 in mouse model of paraneoplastic retinopathy.

TRPM1 is not essential for the development of hearing or balance in the mouse model and it is unlikely that TRPM1 is the hair cell mechanotransduction channel.
Title: Transient receptor potential melastatin 1: a hair cell transduction channel candidate.

The data indicates that the A1068T mutant TRPM1 acts as a dominant negative with respect to TRPM1 channel function.
Title: Depolarizing bipolar cell dysfunction due to a Trpm1 point mutation.

The following Trpm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15305	NM_018752.3 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu15305	XM_017322013.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu81947	XM_017322012.2 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu15260	NM_001039104.2 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$958.30 $1369.00
View Old Accession Versions >>
OMu81947	XM_017322012.1	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpm1 ( NM_018752.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061222.3 Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15305
Clone ID Related Accession (Same CDS sequence)	XM_017322013.1 , NM_018752.3
Accession Version	NM_018752.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY100491.1, AK148275.1, AK164395.1, AF047714.1, AK190606.1 and BB763853.1. On Mar 14, 2008 this sequence version replaced NM_018752.2. Transcript Variant: This variant (TRPM1-S, PMID: 19966281) encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF047714.1, AK148275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164141 [ECO:0000348] ##Evidence-Data-END##

ATGTACATCC GAGTCTCCTA CGACACCAAG CCAGATTCCC TGCTCCACCT CATGGTGAAG 
GACTGGCAGC TGGAGCTCCC GAAGCTCTTG ATATCTGTGC ACGGAGGCCT CCAAAGCTTC 
GAGATGCAGC CCAAACTGAA GCAGGTGTTT GGGAAAGGTC TGATCAAGGC TGCCATGACC 
ACGGGGGCGT GGATCTTCAC CGGGGGTGTG AGCACTGGTG TCGTCAGCCA TGTGGGGGAT 
GCCTTGAAAG ACCACTCCTC CAAGTCCAGA GGCCGGCTCT GTGCTATAGG AATTGCTCCC 
TGGGGCATGG TGGAGAACAA GGAAGACCTG ATTGGAAAAG ATGTAACAAG AGTCTATCAG 
ACCATGTCCA ACCCTCTGAG CAAGCTCTCT GTGCTCAACA ATTCCCACAC TCACTTCATC 
TTGGCTGACA ACGGCACCCT GGGCAAGTAT GGTGCTGAGG TGAAGCTTCG AAGACAGCTG 
GAAAAACACA TCTCCCTGCA GAAGATCAAC ACAAGGCTGG GCCAGGGTGT ACCTGTCGTG 
GGCCTAGTGG TAGAAGGTGG TCCTAACGTG GTTTCTATCG TCCTGGAGTA TCTCAAAGAA 
GACCCTCCTG TCCCTGTGGT GGTTTGCGAT GGCAGTGGAC GTGCCTCTGA CATTTTGTCC 
TTCGCACACA AATACTGCGA CGAAGGAGGA GTCATAAACG AGTCCCTGCG GGACCAGCTT 
CTAGTTACCA TTCAGAAAAC ATTTAATTAC AGCAAGTCCC AGTCGTATCA GCTGTTTGCA 
ATTATCATGG AGTGCATGAA GAAGAAAGAA CTCGTCACTG TGTTTCGGAT GGGTTCCGAG 
GGTCAGCAAG ATGTCGAGAT GGCAATTTTA ACTGCCTTGC TCAAAGGAAC CAACGCATCA 
GCTCCAGATC AGCTGAGCTT GGCCCTGGCT TGGAACCGGG TCGACATAGC GCGAAGCCAG 
ATCTTCGTCT TTGGCCCACA CTGGCCGCCA CTGGGAAGCC TGGCCCCTCC TGTGGACACC 
AAAGCCACAG AGAAGGAAAA GAAGCCACCC ACAGCCACCA CCAAGGGGAG AGGAAAAGGA 
AAAGGCAAGA AGAAAGGCAA AGTGAAAGAG GAAGTGGAGG AAGAGACGGA CCCCCGGAAG 
CTTGAGCTGC TCAACTGGGT GAATGCCCTG GAGCAAGCCA TGCTGGATGC TCTTGTCCTA 
GATCGGGTGG ACTTTGTAAA GCTCCTGATT GAAAACGGAG TGAACATGCA GCATTTCCTC 
ACCATCCCGA GGCTGGAGGA GCTATACAAC ACCAGACTGG GCCCACCAAA CACCCTTCAT 
CTGCTGGTGC GGGATGTAAA GAAGAGCAAC CTTCCACCTG ATTACCACAT CAGCCTCATT 
GATATAGGAC TGGTGCTGGA GTACCTCATG GGCGGTGCCT ACCGCTGCAA CTACACTCGG 
AAAAGCTTCC GGACTCTCTA CAACAACTTG TTTGGCCCTA AGAGGGTAGA GCTCAGCAGA 
CACACAGTGT CCTGTGCCTC CCAGAGTAAC ATGTGGTTCC TTGATGTGCT TCCCCAAAAG 
CCCACCTGTG CAGAATGCAA CTCTTCACCT CACCTGTCCC AAACTGACAT CACCCCACCT 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061222.3
CDS	439..2067
Translation	MYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQSFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVVSHVGDALKDHSSKSRGRLCAIGIAPWGMVENKEDLIGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRQLEKHISLQKINTRLGQGVPVVGLVVEGGPNVVSIVLEYLKEDPPVPVVVCDGSGRASDILSFAHKYCDEGGVINESLRDQLLVTIQKTFNYSKSQSYQLFAIIMECMKKKELVTVFRMGSEGQQDVEMAILTALLKGTNASAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPVDTKATEKEKKPPTATTKGRGKGKGKKKGKVKEEVEEETDPRKLELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKSFRTLYNNLFGPKRVELSRHTVSCASQSNMWFLDVLPQKPTCAECNSSPHLSQTDITPPLP

Target ORF information:

RefSeq Version	NM_018752.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018752.3

ORF Insert Sequence:

ATGTACATCC GAGTCTCCTA CGACACCAAG CCAGATTCCC TGCTCCACCT CATGGTGAAG 
GACTGGCAGC TGGAGCTCCC GAAGCTCTTG ATATCTGTGC ACGGAGGCCT CCAAAGCTTC 
GAGATGCAGC CCAAACTGAA GCAGGTGTTT GGGAAAGGTC TGATCAAGGC TGCCATGACC 
ACGGGGGCGT GGATCTTCAC CGGGGGTGTG AGCACTGGTG TCGTCAGCCA TGTGGGGGAT 
GCCTTGAAAG ACCACTCCTC CAAGTCCAGA GGCCGGCTCT GTGCTATAGG AATTGCTCCC 
TGGGGCATGG TGGAGAACAA GGAAGACCTG ATTGGAAAAG ATGTAACAAG AGTCTATCAG 
ACCATGTCCA ACCCTCTGAG CAAGCTCTCT GTGCTCAACA ATTCCCACAC TCACTTCATC 
TTGGCTGACA ACGGCACCCT GGGCAAGTAT GGTGCTGAGG TGAAGCTTCG AAGACAGCTG 
GAAAAACACA TCTCCCTGCA GAAGATCAAC ACAAGGCTGG GCCAGGGTGT ACCTGTCGTG 
GGCCTAGTGG TAGAAGGTGG TCCTAACGTG GTTTCTATCG TCCTGGAGTA TCTCAAAGAA 
GACCCTCCTG TCCCTGTGGT GGTTTGCGAT GGCAGTGGAC GTGCCTCTGA CATTTTGTCC 
TTCGCACACA AATACTGCGA CGAAGGAGGA GTCATAAACG AGTCCCTGCG GGACCAGCTT 
CTAGTTACCA TTCAGAAAAC ATTTAATTAC AGCAAGTCCC AGTCGTATCA GCTGTTTGCA 
ATTATCATGG AGTGCATGAA GAAGAAAGAA CTCGTCACTG TGTTTCGGAT GGGTTCCGAG 
GGTCAGCAAG ATGTCGAGAT GGCAATTTTA ACTGCCTTGC TCAAAGGAAC CAACGCATCA 
GCTCCAGATC AGCTGAGCTT GGCCCTGGCT TGGAACCGGG TCGACATAGC GCGAAGCCAG 
ATCTTCGTCT TTGGCCCACA CTGGCCGCCA CTGGGAAGCC TGGCCCCTCC TGTGGACACC 
AAAGCCACAG AGAAGGAAAA GAAGCCACCC ACAGCCACCA CCAAGGGGAG AGGAAAAGGA 
AAAGGCAAGA AGAAAGGCAA AGTGAAAGAG GAAGTGGAGG AAGAGACGGA CCCCCGGAAG 
CTTGAGCTGC TCAACTGGGT GAATGCCCTG GAGCAAGCCA TGCTGGATGC TCTTGTCCTA 
GATCGGGTGG ACTTTGTAAA GCTCCTGATT GAAAACGGAG TGAACATGCA GCATTTCCTC 
ACCATCCCGA GGCTGGAGGA GCTATACAAC ACCAGACTGG GCCCACCAAA CACCCTTCAT 
CTGCTGGTGC GGGATGTAAA GAAGAGCAAC CTTCCACCTG ATTACCACAT CAGCCTCATT 
GATATAGGAC TGGTGCTGGA GTACCTCATG GGCGGTGCCT ACCGCTGCAA CTACACTCGG 
AAAAGCTTCC GGACTCTCTA CAACAACTTG TTTGGCCCTA AGAGGGTAGA GCTCAGCAGA 
CACACAGTGT CCTGTGCCTC CCAGAGTAAC ATGTGGTTCC TTGATGTGCT TCCCCAAAAG 
CCCACCTGTG CAGAATGCAA CTCTTCACCT CACCTGTCCC AAACTGACAT CACCCCACCT 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm1 ( XM_017322013.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177502.1 Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu15305
Clone ID Related Accession (Same CDS sequence)	XM_017322013.1 , NM_018752.3
Accession Version	XM_017322013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATCC GAGTCTCCTA CGACACCAAG CCAGATTCCC TGCTCCACCT CATGGTGAAG 
GACTGGCAGC TGGAGCTCCC GAAGCTCTTG ATATCTGTGC ACGGAGGCCT CCAAAGCTTC 
GAGATGCAGC CCAAACTGAA GCAGGTGTTT GGGAAAGGTC TGATCAAGGC TGCCATGACC 
ACGGGGGCGT GGATCTTCAC CGGGGGTGTG AGCACTGGTG TCGTCAGCCA TGTGGGGGAT 
GCCTTGAAAG ACCACTCCTC CAAGTCCAGA GGCCGGCTCT GTGCTATAGG AATTGCTCCC 
TGGGGCATGG TGGAGAACAA GGAAGACCTG ATTGGAAAAG ATGTAACAAG AGTCTATCAG 
ACCATGTCCA ACCCTCTGAG CAAGCTCTCT GTGCTCAACA ATTCCCACAC TCACTTCATC 
TTGGCTGACA ACGGCACCCT GGGCAAGTAT GGTGCTGAGG TGAAGCTTCG AAGACAGCTG 
GAAAAACACA TCTCCCTGCA GAAGATCAAC ACAAGGCTGG GCCAGGGTGT ACCTGTCGTG 
GGCCTAGTGG TAGAAGGTGG TCCTAACGTG GTTTCTATCG TCCTGGAGTA TCTCAAAGAA 
GACCCTCCTG TCCCTGTGGT GGTTTGCGAT GGCAGTGGAC GTGCCTCTGA CATTTTGTCC 
TTCGCACACA AATACTGCGA CGAAGGAGGA GTCATAAACG AGTCCCTGCG GGACCAGCTT 
CTAGTTACCA TTCAGAAAAC ATTTAATTAC AGCAAGTCCC AGTCGTATCA GCTGTTTGCA 
ATTATCATGG AGTGCATGAA GAAGAAAGAA CTCGTCACTG TGTTTCGGAT GGGTTCCGAG 
GGTCAGCAAG ATGTCGAGAT GGCAATTTTA ACTGCCTTGC TCAAAGGAAC CAACGCATCA 
GCTCCAGATC AGCTGAGCTT GGCCCTGGCT TGGAACCGGG TCGACATAGC GCGAAGCCAG 
ATCTTCGTCT TTGGCCCACA CTGGCCGCCA CTGGGAAGCC TGGCCCCTCC TGTGGACACC 
AAAGCCACAG AGAAGGAAAA GAAGCCACCC ACAGCCACCA CCAAGGGGAG AGGAAAAGGA 
AAAGGCAAGA AGAAAGGCAA AGTGAAAGAG GAAGTGGAGG AAGAGACGGA CCCCCGGAAG 
CTTGAGCTGC TCAACTGGGT GAATGCCCTG GAGCAAGCCA TGCTGGATGC TCTTGTCCTA 
GATCGGGTGG ACTTTGTAAA GCTCCTGATT GAAAACGGAG TGAACATGCA GCATTTCCTC 
ACCATCCCGA GGCTGGAGGA GCTATACAAC ACCAGACTGG GCCCACCAAA CACCCTTCAT 
CTGCTGGTGC GGGATGTAAA GAAGAGCAAC CTTCCACCTG ATTACCACAT CAGCCTCATT 
GATATAGGAC TGGTGCTGGA GTACCTCATG GGCGGTGCCT ACCGCTGCAA CTACACTCGG 
AAAAGCTTCC GGACTCTCTA CAACAACTTG TTTGGCCCTA AGAGGGTAGA GCTCAGCAGA 
CACACAGTGT CCTGTGCCTC CCAGAGTAAC ATGTGGTTCC TTGATGTGCT TCCCCAAAAG 
CCCACCTGTG CAGAATGCAA CTCTTCACCT CACCTGTCCC AAACTGACAT CACCCCACCT 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177502.1
CDS	362..1990
Translation	MYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQSFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVVSHVGDALKDHSSKSRGRLCAIGIAPWGMVENKEDLIGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRQLEKHISLQKINTRLGQGVPVVGLVVEGGPNVVSIVLEYLKEDPPVPVVVCDGSGRASDILSFAHKYCDEGGVINESLRDQLLVTIQKTFNYSKSQSYQLFAIIMECMKKKELVTVFRMGSEGQQDVEMAILTALLKGTNASAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPVDTKATEKEKKPPTATTKGRGKGKGKKKGKVKEEVEEETDPRKLELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKSFRTLYNNLFGPKRVELSRHTVSCASQSNMWFLDVLPQKPTCAECNSSPHLSQTDITPPLP

Target ORF information:

RefSeq Version	XM_017322013.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322013.1

ORF Insert Sequence:

ATGTACATCC GAGTCTCCTA CGACACCAAG CCAGATTCCC TGCTCCACCT CATGGTGAAG 
GACTGGCAGC TGGAGCTCCC GAAGCTCTTG ATATCTGTGC ACGGAGGCCT CCAAAGCTTC 
GAGATGCAGC CCAAACTGAA GCAGGTGTTT GGGAAAGGTC TGATCAAGGC TGCCATGACC 
ACGGGGGCGT GGATCTTCAC CGGGGGTGTG AGCACTGGTG TCGTCAGCCA TGTGGGGGAT 
GCCTTGAAAG ACCACTCCTC CAAGTCCAGA GGCCGGCTCT GTGCTATAGG AATTGCTCCC 
TGGGGCATGG TGGAGAACAA GGAAGACCTG ATTGGAAAAG ATGTAACAAG AGTCTATCAG 
ACCATGTCCA ACCCTCTGAG CAAGCTCTCT GTGCTCAACA ATTCCCACAC TCACTTCATC 
TTGGCTGACA ACGGCACCCT GGGCAAGTAT GGTGCTGAGG TGAAGCTTCG AAGACAGCTG 
GAAAAACACA TCTCCCTGCA GAAGATCAAC ACAAGGCTGG GCCAGGGTGT ACCTGTCGTG 
GGCCTAGTGG TAGAAGGTGG TCCTAACGTG GTTTCTATCG TCCTGGAGTA TCTCAAAGAA 
GACCCTCCTG TCCCTGTGGT GGTTTGCGAT GGCAGTGGAC GTGCCTCTGA CATTTTGTCC 
TTCGCACACA AATACTGCGA CGAAGGAGGA GTCATAAACG AGTCCCTGCG GGACCAGCTT 
CTAGTTACCA TTCAGAAAAC ATTTAATTAC AGCAAGTCCC AGTCGTATCA GCTGTTTGCA 
ATTATCATGG AGTGCATGAA GAAGAAAGAA CTCGTCACTG TGTTTCGGAT GGGTTCCGAG 
GGTCAGCAAG ATGTCGAGAT GGCAATTTTA ACTGCCTTGC TCAAAGGAAC CAACGCATCA 
GCTCCAGATC AGCTGAGCTT GGCCCTGGCT TGGAACCGGG TCGACATAGC GCGAAGCCAG 
ATCTTCGTCT TTGGCCCACA CTGGCCGCCA CTGGGAAGCC TGGCCCCTCC TGTGGACACC 
AAAGCCACAG AGAAGGAAAA GAAGCCACCC ACAGCCACCA CCAAGGGGAG AGGAAAAGGA 
AAAGGCAAGA AGAAAGGCAA AGTGAAAGAG GAAGTGGAGG AAGAGACGGA CCCCCGGAAG 
CTTGAGCTGC TCAACTGGGT GAATGCCCTG GAGCAAGCCA TGCTGGATGC TCTTGTCCTA 
GATCGGGTGG ACTTTGTAAA GCTCCTGATT GAAAACGGAG TGAACATGCA GCATTTCCTC 
ACCATCCCGA GGCTGGAGGA GCTATACAAC ACCAGACTGG GCCCACCAAA CACCCTTCAT 
CTGCTGGTGC GGGATGTAAA GAAGAGCAAC CTTCCACCTG ATTACCACAT CAGCCTCATT 
GATATAGGAC TGGTGCTGGA GTACCTCATG GGCGGTGCCT ACCGCTGCAA CTACACTCGG 
AAAAGCTTCC GGACTCTCTA CAACAACTTG TTTGGCCCTA AGAGGGTAGA GCTCAGCAGA 
CACACAGTGT CCTGTGCCTC CCAGAGTAAC ATGTGGTTCC TTGATGTGCT TCCCCAAAAG 
CCCACCTGTG CAGAATGCAA CTCTTCACCT CACCTGTCCC AAACTGACAT CACCCCACCT 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm1 ( XM_017322012.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177501.1 Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81947
Clone ID Related Accession (Same CDS sequence)	XM_017322012.2 , XM_017322012.1
Accession Version	XM_017322012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGG CCGTGCTCAT GAAGCGCCAG AAGATGGCGG TCTTCCTGTG GCAGCGTGGG 
GAAGAGTGCA TGGCGAAGGC CTTGGTGGCC TGCAAGCTCT ACAAGGCCAT GGCCCACGAG 
TCCTCCGAGA GCGAGCTGGT GGATGACATC TCCCAGGATC TGGACAACAA TTCCAAAGAT 
TTTGGCCAGC TCGCTGTGGA GCTGTTAGAC CAGTCTTACA AGCACGATGA GCAGGTTGCC 
ATGAAACTTC TGACCTACGA GCTCAAGAAC TGGAGCAACT CAACCTGCCT GAAGCTGGCT 
GTGGCAGCCA AGCACCGGGA CTTCATTGCT CACACCTGCA GCCAGATGCT GCTGACAGAC 
ATGTGGATGG GAAGGCTTCG CATGAGGAAG AACCCTGGCC TGAAGGTCAT CATGGGGATC 
CTTATCCCAC CCACCATCTT GTTTTTGGAA TTCCGTACAT ATGATGACTT CTCCTATCAA 
ACATCCAAGG AGAACGAAGA TGGCAAAGAA AAGGAAGAAG AGAATGTGAT TTCCTACTTG 
GGCTACTTGC TGCTGTTCAA TTATGTCATC CTGGTGCGGA TGGATGGATG GCCATCACCC 
CAAGAATGGA TTGTCATCTC CTACATTGTG AGCCTTGCAT TGGAGAAAAT ACGAGAGATC 
CTCATGTCGG AACCAGGCAA ACTCAGCCAG AAAATCAAAG TGTGGCTCCA GGAGTACTGG 
AACATCACAG ACCTCGTGGC CATCTCCATG TTCATGGTTG GGGCCATCCT TCGTCTCCAG 
AGCCAGCCAT ACATGGGCTA TGGTCGGGTC ATCTACTGTG TGGATATCAT CCTCTGGTAC 
ATCCGAGTGT TAGACATCTT TGGTGTCAAC AAATATCTTG GTCCCTATGT GATGATGATT 
GGAAAGATGA TGATTGACAT GCTCTACTTT GTGGTCATTA TGCTGGTTGT ACTGATGAGT 
TTTGGAGTGG CTCGGCAAGC CATCTTGCAT CCGGAGGAGA AGCCTTCCTG GAAACTGGCC 
CGAAACATCT TCTACATGCC ATACTGGATG ATCTACGGAG AGGTGTTTGC AGACCAGATA 
GACCTCTACG CCATGGAAAT CAACCCTCCT TGTGGTGAGA ATCTCTATGA TGAGGAAGGC 
AAGAGGCTCC CTCCCTGCAT CCCTGGTGCC TGGCTCACAC CTGCCCTCAT GGCCTGTTAT 
CTCCTGGTGG CCAATATCCT GCTGGTCAAC CTACTGATTG CTGTTTTCAA CAATACCTTC 
TTTGAAGTCA AGTCAATATC CAATCAAGTG TGGAAGTTCC AGCGATACCA GCTGATCATG 
ACATTTCATG ACAGGCCGGT CCTGCCCCCA CCAATGATCA TCTTAAGTCA CATCTATATC 
ATCATTATGC GCCTCAGCGG TCGCTGCAGA AAAAAGAGAG AGGGGGACCA GGAGGAGCGG 
GACCGTGGGT TGAAGTTGTT CCTTAGCGAC GAAGAACTGA AAAAACTGCA TGAGTTTGAG 
GAGCAGTGCG TGCAGGAGCA CTTCCGGGAA AAGGAGGATG AGCAGCAGTC GTCCAGCGAC 
GAGCGCATCC GGGTCACAAG CGAGAGGGTT GAAAATATGT CAATGAGATT GGAGGAAATC 
AACGAAAGAG AAAATTTCAT GAAAACTTCT CTGCAGACTG TTGACCTTCG GCTCTCTCAG 
CTGGAGGAGC TATCTGGCAG GATGGTGAGC GCTCTGGAGA ATCTGGCAGG CATCGACAGG 
TCTGATCTGA TCCAGGCTCG ATCTCGAGCA TCCTCCGAAT GCGAGGCCAC CTACCTGCTG 
CGGCAGAGCA GCATCAACAG TGCCGACGGC TACAGCTTGT ACCGCTACCA TTTCAATGGC 
GAGGAGCTTC TGTTTGAGGA GCCTGCTCTC TCCACTTCGC CAGGGACAGC GTTCCGGAAA 
AAAACCTACT CCTTTCGTGT GAAGGATGAG GATGCAAAGT CGCACCTGGA CCAGCCCAGC 
AACCTGCACC ACACCCCTGG CCCCAGCCCA CCTGCCACGC CAGGCCGCAG CCGGCTCGCC 
CTTGAAGGTC CCTTGAGCAC AGAGCTGAGA CCTGGATCGG ACCCTGGCAT CTCCGCAGGC 
GAGTTCGATC CGCGGGCGGA TTTCAAGAGC ACGGAAGCTG CACCAAGTCT GAACGCAGCG 
GGTGTTACAG GCACTCAACT GACTGTCGAA AGCACCGATT CCCACCCTCT GCGGGAGAGC 
AAACTCGTGC GCTACTACCC CGGAGACCCT AACACCTACA AAACAATGAA GTCCAGAAGC 
TTCGTCTATA CTGAAGGAAG AAAGCTAGTC CGAGGTCTTA GCAACTGGAG TGCAGAGTAC 
AGTTCAATCA TGGACCAAGC ATGGAACGCG ACAGAATGGA GATGTCAAGT TCAAAGGATC 
ACGCGCTCCC GCAGCACAGA CATCCCATAC ATCGTGTCCG AAGCAGCCTC GCAAGACGAG 
CTTGAAGATG AGCACAGAGG ATCTCTTCTG GATCCTCAGA TCTCCCGTTC AGCCCTCACC 
GTCTCCGACA GGCCAGAAAA GGAAAACTTA CTCTCTGTGA AGCCACATCA GACTTTAGGA 
TTCCCCTGCC TACGGTCAAG AAGTTTACAT GGCCGTCCTA GGAGTGCTGA GCCCGCTCCT 
AGCAAATTAG ACAGGGCAGG ACATGCCAGC AGCACTAGCA ACTTAGCAGT TATGTCAGTT 
GTTCCAGAGG GACAAAACAC CCAGCAAGAG AAAAGAAGCG CGGAAACTGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177501.1
CDS	253..3009
Translation	MVWAVLMKRQKMAVFLWQRGEECMAKALVACKLYKAMAHESSESELVDDISQDLDNNSKDFGQLAVELLDQSYKHDEQVAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILIPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENVISYLGYLLLFNYVILVRMDGWPSPQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISMFMVGAILRLQSQPYMGYGRVIYCVDIILWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKKLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERENFMKTSLQTVDLRLSQLEELSGRMVSALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEEPALSTSPGTAFRKKTYSFRVKDEDAKSHLDQPSNLHHTPGPSPPATPGRSRLALEGPLSTELRPGSDPGISAGEFDPRADFKSTEAAPSLNAAGVTGTQLTVESTDSHPLRESKLVRYYPGDPNTYKTMKSRSFVYTEGRKLVRGLSNWSAEYSSIMDQAWNATEWRCQVQRITRSRSTDIPYIVSEAASQDELEDEHRGSLLDPQISRSALTVSDRPEKENLLSVKPHQTLGFPCLRSRSLHGRPRSAEPAPSKLDRAGHASSTSNLAVMSVVPEGQNTQQEKRSAETEC

Target ORF information:

RefSeq Version	XM_017322012.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322012.2

ORF Insert Sequence:

ATGGTATGGG CCGTGCTCAT GAAGCGCCAG AAGATGGCGG TCTTCCTGTG GCAGCGTGGG 
GAAGAGTGCA TGGCGAAGGC CTTGGTGGCC TGCAAGCTCT ACAAGGCCAT GGCCCACGAG 
TCCTCCGAGA GCGAGCTGGT GGATGACATC TCCCAGGATC TGGACAACAA TTCCAAAGAT 
TTTGGCCAGC TCGCTGTGGA GCTGTTAGAC CAGTCTTACA AGCACGATGA GCAGGTTGCC 
ATGAAACTTC TGACCTACGA GCTCAAGAAC TGGAGCAACT CAACCTGCCT GAAGCTGGCT 
GTGGCAGCCA AGCACCGGGA CTTCATTGCT CACACCTGCA GCCAGATGCT GCTGACAGAC 
ATGTGGATGG GAAGGCTTCG CATGAGGAAG AACCCTGGCC TGAAGGTCAT CATGGGGATC 
CTTATCCCAC CCACCATCTT GTTTTTGGAA TTCCGTACAT ATGATGACTT CTCCTATCAA 
ACATCCAAGG AGAACGAAGA TGGCAAAGAA AAGGAAGAAG AGAATGTGAT TTCCTACTTG 
GGCTACTTGC TGCTGTTCAA TTATGTCATC CTGGTGCGGA TGGATGGATG GCCATCACCC 
CAAGAATGGA TTGTCATCTC CTACATTGTG AGCCTTGCAT TGGAGAAAAT ACGAGAGATC 
CTCATGTCGG AACCAGGCAA ACTCAGCCAG AAAATCAAAG TGTGGCTCCA GGAGTACTGG 
AACATCACAG ACCTCGTGGC CATCTCCATG TTCATGGTTG GGGCCATCCT TCGTCTCCAG 
AGCCAGCCAT ACATGGGCTA TGGTCGGGTC ATCTACTGTG TGGATATCAT CCTCTGGTAC 
ATCCGAGTGT TAGACATCTT TGGTGTCAAC AAATATCTTG GTCCCTATGT GATGATGATT 
GGAAAGATGA TGATTGACAT GCTCTACTTT GTGGTCATTA TGCTGGTTGT ACTGATGAGT 
TTTGGAGTGG CTCGGCAAGC CATCTTGCAT CCGGAGGAGA AGCCTTCCTG GAAACTGGCC 
CGAAACATCT TCTACATGCC ATACTGGATG ATCTACGGAG AGGTGTTTGC AGACCAGATA 
GACCTCTACG CCATGGAAAT CAACCCTCCT TGTGGTGAGA ATCTCTATGA TGAGGAAGGC 
AAGAGGCTCC CTCCCTGCAT CCCTGGTGCC TGGCTCACAC CTGCCCTCAT GGCCTGTTAT 
CTCCTGGTGG CCAATATCCT GCTGGTCAAC CTACTGATTG CTGTTTTCAA CAATACCTTC 
TTTGAAGTCA AGTCAATATC CAATCAAGTG TGGAAGTTCC AGCGATACCA GCTGATCATG 
ACATTTCATG ACAGGCCGGT CCTGCCCCCA CCAATGATCA TCTTAAGTCA CATCTATATC 
ATCATTATGC GCCTCAGCGG TCGCTGCAGA AAAAAGAGAG AGGGGGACCA GGAGGAGCGG 
GACCGTGGGT TGAAGTTGTT CCTTAGCGAC GAAGAACTGA AAAAACTGCA TGAGTTTGAG 
GAGCAGTGCG TGCAGGAGCA CTTCCGGGAA AAGGAGGATG AGCAGCAGTC GTCCAGCGAC 
GAGCGCATCC GGGTCACAAG CGAGAGGGTT GAAAATATGT CAATGAGATT GGAGGAAATC 
AACGAAAGAG AAAATTTCAT GAAAACTTCT CTGCAGACTG TTGACCTTCG GCTCTCTCAG 
CTGGAGGAGC TATCTGGCAG GATGGTGAGC GCTCTGGAGA ATCTGGCAGG CATCGACAGG 
TCTGATCTGA TCCAGGCTCG ATCTCGAGCA TCCTCCGAAT GCGAGGCCAC CTACCTGCTG 
CGGCAGAGCA GCATCAACAG TGCCGACGGC TACAGCTTGT ACCGCTACCA TTTCAATGGC 
GAGGAGCTTC TGTTTGAGGA GCCTGCTCTC TCCACTTCGC CAGGGACAGC GTTCCGGAAA 
AAAACCTACT CCTTTCGTGT GAAGGATGAG GATGCAAAGT CGCACCTGGA CCAGCCCAGC 
AACCTGCACC ACACCCCTGG CCCCAGCCCA CCTGCCACGC CAGGCCGCAG CCGGCTCGCC 
CTTGAAGGTC CCTTGAGCAC AGAGCTGAGA CCTGGATCGG ACCCTGGCAT CTCCGCAGGC 
GAGTTCGATC CGCGGGCGGA TTTCAAGAGC ACGGAAGCTG CACCAAGTCT GAACGCAGCG 
GGTGTTACAG GCACTCAACT GACTGTCGAA AGCACCGATT CCCACCCTCT GCGGGAGAGC 
AAACTCGTGC GCTACTACCC CGGAGACCCT AACACCTACA AAACAATGAA GTCCAGAAGC 
TTCGTCTATA CTGAAGGAAG AAAGCTAGTC CGAGGTCTTA GCAACTGGAG TGCAGAGTAC 
AGTTCAATCA TGGACCAAGC ATGGAACGCG ACAGAATGGA GATGTCAAGT TCAAAGGATC 
ACGCGCTCCC GCAGCACAGA CATCCCATAC ATCGTGTCCG AAGCAGCCTC GCAAGACGAG 
CTTGAAGATG AGCACAGAGG ATCTCTTCTG GATCCTCAGA TCTCCCGTTC AGCCCTCACC 
GTCTCCGACA GGCCAGAAAA GGAAAACTTA CTCTCTGTGA AGCCACATCA GACTTTAGGA 
TTCCCCTGCC TACGGTCAAG AAGTTTACAT GGCCGTCCTA GGAGTGCTGA GCCCGCTCCT 
AGCAAATTAG ACAGGGCAGG ACATGCCAGC AGCACTAGCA ACTTAGCAGT TATGTCAGTT 
GTTCCAGAGG GACAAAACAC CCAGCAAGAG AAAAGAAGCG CGGAAACTGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm1 ( XM_017322012.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177501.1 Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X1, mRNA.

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CloneID	OMu81947
Clone ID Related Accession (Same CDS sequence)	XM_017322012.2 , XM_017322012.1
Accession Version	XM_017322012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGG CCGTGCTCAT GAAGCGCCAG AAGATGGCGG TCTTCCTGTG GCAGCGTGGG 
GAAGAGTGCA TGGCGAAGGC CTTGGTGGCC TGCAAGCTCT ACAAGGCCAT GGCCCACGAG 
TCCTCCGAGA GCGAGCTGGT GGATGACATC TCCCAGGATC TGGACAACAA TTCCAAAGAT 
TTTGGCCAGC TCGCTGTGGA GCTGTTAGAC CAGTCTTACA AGCACGATGA GCAGGTTGCC 
ATGAAACTTC TGACCTACGA GCTCAAGAAC TGGAGCAACT CAACCTGCCT GAAGCTGGCT 
GTGGCAGCCA AGCACCGGGA CTTCATTGCT CACACCTGCA GCCAGATGCT GCTGACAGAC 
ATGTGGATGG GAAGGCTTCG CATGAGGAAG AACCCTGGCC TGAAGGTCAT CATGGGGATC 
CTTATCCCAC CCACCATCTT GTTTTTGGAA TTCCGTACAT ATGATGACTT CTCCTATCAA 
ACATCCAAGG AGAACGAAGA TGGCAAAGAA AAGGAAGAAG AGAATGTGAT TTCCTACTTG 
GGCTACTTGC TGCTGTTCAA TTATGTCATC CTGGTGCGGA TGGATGGATG GCCATCACCC 
CAAGAATGGA TTGTCATCTC CTACATTGTG AGCCTTGCAT TGGAGAAAAT ACGAGAGATC 
CTCATGTCGG AACCAGGCAA ACTCAGCCAG AAAATCAAAG TGTGGCTCCA GGAGTACTGG 
AACATCACAG ACCTCGTGGC CATCTCCATG TTCATGGTTG GGGCCATCCT TCGTCTCCAG 
AGCCAGCCAT ACATGGGCTA TGGTCGGGTC ATCTACTGTG TGGATATCAT CCTCTGGTAC 
ATCCGAGTGT TAGACATCTT TGGTGTCAAC AAATATCTTG GTCCCTATGT GATGATGATT 
GGAAAGATGA TGATTGACAT GCTCTACTTT GTGGTCATTA TGCTGGTTGT ACTGATGAGT 
TTTGGAGTGG CTCGGCAAGC CATCTTGCAT CCGGAGGAGA AGCCTTCCTG GAAACTGGCC 
CGAAACATCT TCTACATGCC ATACTGGATG ATCTACGGAG AGGTGTTTGC AGACCAGATA 
GACCTCTACG CCATGGAAAT CAACCCTCCT TGTGGTGAGA ATCTCTATGA TGAGGAAGGC 
AAGAGGCTCC CTCCCTGCAT CCCTGGTGCC TGGCTCACAC CTGCCCTCAT GGCCTGTTAT 
CTCCTGGTGG CCAATATCCT GCTGGTCAAC CTACTGATTG CTGTTTTCAA CAATACCTTC 
TTTGAAGTCA AGTCAATATC CAATCAAGTG TGGAAGTTCC AGCGATACCA GCTGATCATG 
ACATTTCATG ACAGGCCGGT CCTGCCCCCA CCAATGATCA TCTTAAGTCA CATCTATATC 
ATCATTATGC GCCTCAGCGG TCGCTGCAGA AAAAAGAGAG AGGGGGACCA GGAGGAGCGG 
GACCGTGGGT TGAAGTTGTT CCTTAGCGAC GAAGAACTGA AAAAACTGCA TGAGTTTGAG 
GAGCAGTGCG TGCAGGAGCA CTTCCGGGAA AAGGAGGATG AGCAGCAGTC GTCCAGCGAC 
GAGCGCATCC GGGTCACAAG CGAGAGGGTT GAAAATATGT CAATGAGATT GGAGGAAATC 
AACGAAAGAG AAAATTTCAT GAAAACTTCT CTGCAGACTG TTGACCTTCG GCTCTCTCAG 
CTGGAGGAGC TATCTGGCAG GATGGTGAGC GCTCTGGAGA ATCTGGCAGG CATCGACAGG 
TCTGATCTGA TCCAGGCTCG ATCTCGAGCA TCCTCCGAAT GCGAGGCCAC CTACCTGCTG 
CGGCAGAGCA GCATCAACAG TGCCGACGGC TACAGCTTGT ACCGCTACCA TTTCAATGGC 
GAGGAGCTTC TGTTTGAGGA GCCTGCTCTC TCCACTTCGC CAGGGACAGC GTTCCGGAAA 
AAAACCTACT CCTTTCGTGT GAAGGATGAG GATGCAAAGT CGCACCTGGA CCAGCCCAGC 
AACCTGCACC ACACCCCTGG CCCCAGCCCA CCTGCCACGC CAGGCCGCAG CCGGCTCGCC 
CTTGAAGGTC CCTTGAGCAC AGAGCTGAGA CCTGGATCGG ACCCTGGCAT CTCCGCAGGC 
GAGTTCGATC CGCGGGCGGA TTTCAAGAGC ACGGAAGCTG CACCAAGTCT GAACGCAGCG 
GGTGTTACAG GCACTCAACT GACTGTCGAA AGCACCGATT CCCACCCTCT GCGGGAGAGC 
AAACTCGTGC GCTACTACCC CGGAGACCCT AACACCTACA AAACAATGAA GTCCAGAAGC 
TTCGTCTATA CTGAAGGAAG AAAGCTAGTC CGAGGTCTTA GCAACTGGAG TGCAGAGTAC 
AGTTCAATCA TGGACCAAGC ATGGAACGCG ACAGAATGGA GATGTCAAGT TCAAAGGATC 
ACGCGCTCCC GCAGCACAGA CATCCCATAC ATCGTGTCCG AAGCAGCCTC GCAAGACGAG 
CTTGAAGATG AGCACAGAGG ATCTCTTCTG GATCCTCAGA TCTCCCGTTC AGCCCTCACC 
GTCTCCGACA GGCCAGAAAA GGAAAACTTA CTCTCTGTGA AGCCACATCA GACTTTAGGA 
TTCCCCTGCC TACGGTCAAG AAGTTTACAT GGCCGTCCTA GGAGTGCTGA GCCCGCTCCT 
AGCAAATTAG ACAGGGCAGG ACATGCCAGC AGCACTAGCA ACTTAGCAGT TATGTCAGTT 
GTTCCAGAGG GACAAAACAC CCAGCAAGAG AAAAGAAGCG CGGAAACTGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177501.1
CDS	798..3554
Translation	MVWAVLMKRQKMAVFLWQRGEECMAKALVACKLYKAMAHESSESELVDDISQDLDNNSKDFGQLAVELLDQSYKHDEQVAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILIPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENVISYLGYLLLFNYVILVRMDGWPSPQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISMFMVGAILRLQSQPYMGYGRVIYCVDIILWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKKLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERENFMKTSLQTVDLRLSQLEELSGRMVSALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEEPALSTSPGTAFRKKTYSFRVKDEDAKSHLDQPSNLHHTPGPSPPATPGRSRLALEGPLSTELRPGSDPGISAGEFDPRADFKSTEAAPSLNAAGVTGTQLTVESTDSHPLRESKLVRYYPGDPNTYKTMKSRSFVYTEGRKLVRGLSNWSAEYSSIMDQAWNATEWRCQVQRITRSRSTDIPYIVSEAASQDELEDEHRGSLLDPQISRSALTVSDRPEKENLLSVKPHQTLGFPCLRSRSLHGRPRSAEPAPSKLDRAGHASSTSNLAVMSVVPEGQNTQQEKRSAETEC

Target ORF information:

RefSeq Version	XM_017322012.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322012.1

ORF Insert Sequence:

ATGGTATGGG CCGTGCTCAT GAAGCGCCAG AAGATGGCGG TCTTCCTGTG GCAGCGTGGG 
GAAGAGTGCA TGGCGAAGGC CTTGGTGGCC TGCAAGCTCT ACAAGGCCAT GGCCCACGAG 
TCCTCCGAGA GCGAGCTGGT GGATGACATC TCCCAGGATC TGGACAACAA TTCCAAAGAT 
TTTGGCCAGC TCGCTGTGGA GCTGTTAGAC CAGTCTTACA AGCACGATGA GCAGGTTGCC 
ATGAAACTTC TGACCTACGA GCTCAAGAAC TGGAGCAACT CAACCTGCCT GAAGCTGGCT 
GTGGCAGCCA AGCACCGGGA CTTCATTGCT CACACCTGCA GCCAGATGCT GCTGACAGAC 
ATGTGGATGG GAAGGCTTCG CATGAGGAAG AACCCTGGCC TGAAGGTCAT CATGGGGATC 
CTTATCCCAC CCACCATCTT GTTTTTGGAA TTCCGTACAT ATGATGACTT CTCCTATCAA 
ACATCCAAGG AGAACGAAGA TGGCAAAGAA AAGGAAGAAG AGAATGTGAT TTCCTACTTG 
GGCTACTTGC TGCTGTTCAA TTATGTCATC CTGGTGCGGA TGGATGGATG GCCATCACCC 
CAAGAATGGA TTGTCATCTC CTACATTGTG AGCCTTGCAT TGGAGAAAAT ACGAGAGATC 
CTCATGTCGG AACCAGGCAA ACTCAGCCAG AAAATCAAAG TGTGGCTCCA GGAGTACTGG 
AACATCACAG ACCTCGTGGC CATCTCCATG TTCATGGTTG GGGCCATCCT TCGTCTCCAG 
AGCCAGCCAT ACATGGGCTA TGGTCGGGTC ATCTACTGTG TGGATATCAT CCTCTGGTAC 
ATCCGAGTGT TAGACATCTT TGGTGTCAAC AAATATCTTG GTCCCTATGT GATGATGATT 
GGAAAGATGA TGATTGACAT GCTCTACTTT GTGGTCATTA TGCTGGTTGT ACTGATGAGT 
TTTGGAGTGG CTCGGCAAGC CATCTTGCAT CCGGAGGAGA AGCCTTCCTG GAAACTGGCC 
CGAAACATCT TCTACATGCC ATACTGGATG ATCTACGGAG AGGTGTTTGC AGACCAGATA 
GACCTCTACG CCATGGAAAT CAACCCTCCT TGTGGTGAGA ATCTCTATGA TGAGGAAGGC 
AAGAGGCTCC CTCCCTGCAT CCCTGGTGCC TGGCTCACAC CTGCCCTCAT GGCCTGTTAT 
CTCCTGGTGG CCAATATCCT GCTGGTCAAC CTACTGATTG CTGTTTTCAA CAATACCTTC 
TTTGAAGTCA AGTCAATATC CAATCAAGTG TGGAAGTTCC AGCGATACCA GCTGATCATG 
ACATTTCATG ACAGGCCGGT CCTGCCCCCA CCAATGATCA TCTTAAGTCA CATCTATATC 
ATCATTATGC GCCTCAGCGG TCGCTGCAGA AAAAAGAGAG AGGGGGACCA GGAGGAGCGG 
GACCGTGGGT TGAAGTTGTT CCTTAGCGAC GAAGAACTGA AAAAACTGCA TGAGTTTGAG 
GAGCAGTGCG TGCAGGAGCA CTTCCGGGAA AAGGAGGATG AGCAGCAGTC GTCCAGCGAC 
GAGCGCATCC GGGTCACAAG CGAGAGGGTT GAAAATATGT CAATGAGATT GGAGGAAATC 
AACGAAAGAG AAAATTTCAT GAAAACTTCT CTGCAGACTG TTGACCTTCG GCTCTCTCAG 
CTGGAGGAGC TATCTGGCAG GATGGTGAGC GCTCTGGAGA ATCTGGCAGG CATCGACAGG 
TCTGATCTGA TCCAGGCTCG ATCTCGAGCA TCCTCCGAAT GCGAGGCCAC CTACCTGCTG 
CGGCAGAGCA GCATCAACAG TGCCGACGGC TACAGCTTGT ACCGCTACCA TTTCAATGGC 
GAGGAGCTTC TGTTTGAGGA GCCTGCTCTC TCCACTTCGC CAGGGACAGC GTTCCGGAAA 
AAAACCTACT CCTTTCGTGT GAAGGATGAG GATGCAAAGT CGCACCTGGA CCAGCCCAGC 
AACCTGCACC ACACCCCTGG CCCCAGCCCA CCTGCCACGC CAGGCCGCAG CCGGCTCGCC 
CTTGAAGGTC CCTTGAGCAC AGAGCTGAGA CCTGGATCGG ACCCTGGCAT CTCCGCAGGC 
GAGTTCGATC CGCGGGCGGA TTTCAAGAGC ACGGAAGCTG CACCAAGTCT GAACGCAGCG 
GGTGTTACAG GCACTCAACT GACTGTCGAA AGCACCGATT CCCACCCTCT GCGGGAGAGC 
AAACTCGTGC GCTACTACCC CGGAGACCCT AACACCTACA AAACAATGAA GTCCAGAAGC 
TTCGTCTATA CTGAAGGAAG AAAGCTAGTC CGAGGTCTTA GCAACTGGAG TGCAGAGTAC 
AGTTCAATCA TGGACCAAGC ATGGAACGCG ACAGAATGGA GATGTCAAGT TCAAAGGATC 
ACGCGCTCCC GCAGCACAGA CATCCCATAC ATCGTGTCCG AAGCAGCCTC GCAAGACGAG 
CTTGAAGATG AGCACAGAGG ATCTCTTCTG GATCCTCAGA TCTCCCGTTC AGCCCTCACC 
GTCTCCGACA GGCCAGAAAA GGAAAACTTA CTCTCTGTGA AGCCACATCA GACTTTAGGA 
TTCCCCTGCC TACGGTCAAG AAGTTTACAT GGCCGTCCTA GGAGTGCTGA GCCCGCTCCT 
AGCAAATTAG ACAGGGCAGG ACATGCCAGC AGCACTAGCA ACTTAGCAGT TATGTCAGTT 
GTTCCAGAGG GACAAAACAC CCAGCAAGAG AAAAGAAGCG CGGAAACTGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm1 ( NM_001039104.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034193.2 Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant 2, mRNA.

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CloneID	OMu15260
Clone ID Related Accession (Same CDS sequence)	NM_001039104.2
Accession Version	NM_001039104.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY180104.2. On Mar 14, 2008 this sequence version replaced NM_001039104.1. Transcript Variant: This variant (TRPM1-L, PMID: 19966281, also known as Trpm1delta189-1078) differs in the 5' UTR and 5' coding region, and includes several additional exons at the 3' end, compared to variant 1. The resulting isoform (2) has distinct N- and C-termini, and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY180104.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849388 [ECO:0000350] ##Evidence-Data-END##

ATGGGGTCCA TGAGGAAGAT GAGCAGCTCC TTCAAGCGTG GTTCAATCAA GAGCTCCACA 
TCAGGGTCCC AGAAGGGTCA GAAAGCATGG ATAGAGAAGA CATTTTGCAA AAGGGAATGC 
ATCTTTGTAA TTCCCAGTAC AAAAGACCCT AACAGATGTT GCTGTGGTCA GCTCACTAAC 
CAGCACATCC CCCCTCTGCC GAGTGGGGCT CCCAGCACAA CAGGAGAGGA CACCAAGCAG 
GCAGACACGC AGTCCGGGAA ATGGTCTGTC AGCAAACACA CCCAGAGCTA CCCAACAGAC 
TCCTATGGGA TTCTTGAATT CCAGGGTGGG GGTTACTCCA ATAAAGCCAT GTACATCCGA 
GTCTCCTACG ACACCAAGCC AGATTCCCTG CTCCACCTCA TGGTGAAGGA CTGGCAGCTG 
GAGCTCCCGA AGCTCTTGAT ATCTGTGCAC GGAGGCCTCC AAAGCTTCGA GATGCAGCCC 
AAACTGAAGC AGGTGTTTGG GAAAGGTCTG ATCAAGGCTG CCATGACCAC GGGGGCGTGG 
ATCTTCACCG GGGGTGTGAG CACTGGTGTC GTCAGCCATG TGGGGGATGC CTTGAAAGAC 
CACTCCTCCA AGTCCAGAGG CCGGCTCTGT GCTATAGGAA TTGCTCCCTG GGGCATGGTG 
GAGAACAAGG AAGACCTGAT TGGAAAAGAT GTAACAAGAG TCTATCAGAC CATGTCCAAC 
CCTCTGAGCA AGCTCTCTGT GCTCAACAAT TCCCACACTC ACTTCATCTT GGCTGACAAC 
GGCACCCTGG GCAAGTATGG TGCTGAGGTG AAGCTTCGAA GACAGCTGGA AAAACACATC 
TCCCTGCAGA AGATCAACAC AAGGCTGGGC CAGGGTGTAC CTGTCGTGGG CCTAGTGGTA 
GAAGGTGGTC CTAACGTGGT TTCTATCGTC CTGGAGTATC TCAAAGAAGA CCCTCCTGTC 
CCTGTGGTGG TTTGCGATGG CAGTGGACGT GCCTCTGACA TTTTGTCCTT CGCACACAAA 
TACTGCGACG AAGGAGGAGT CATAAACGAG TCCCTGCGGG ACCAGCTTCT AGTTACCATT 
CAGAAAACAT TTAATTACAG CAAGTCCCAG TCGTATCAGC TGTTTGCAAT TATCATGGAG 
TGCATGAAGA AGAAAGAACT CGTCACTGTG TTTCGGATGG GTTCCGAGGG TCAGCAAGAT 
GTCGAGATGG CAATTTTAAC TGCCTTGCTC AAAGGAACCA ACGCATCAGC TCCAGATCAG 
CTGAGCTTGG CCCTGGCTTG GAACCGGGTC GACATAGCGC GAAGCCAGAT CTTCGTCTTT 
GGCCCACACT GGCCGCCACT GGGAAGCCTG GCCCCTCCTG TGGACACCAA AGCCACAGAG 
AAGGAAAAGA AGCCACCCAC AGCCACCACC AAGGGGAGAG GAAAAGGAAA AGGCAAGAAG 
AAAGGCAAAG TGAAAGAGGA AGTGGAGGAA GAGACGGACC CCCGGAAGCT TGAGCTGCTC 
AACTGGGTGA ATGCCCTGGA GCAAGCCATG CTGGATGCTC TTGTCCTAGA TCGGGTGGAC 
TTTGTAAAGC TCCTGATTGA AAACGGAGTG AACATGCAGC ATTTCCTCAC CATCCCGAGG 
CTGGAGGAGC TATACAACAC CAGACTGGGC CCACCAAACA CCCTTCATCT GCTGGTGCGG 
GATGTAAAGA AGAGCAACCT TCCACCTGAT TACCACATCA GCCTCATTGA TATAGGACTG 
GTGCTGGAGT ACCTCATGGG CGGTGCCTAC CGCTGCAACT ACACTCGGAA AAGCTTCCGG 
ACTCTCTACA ACAACTTGTT TGGCCCTAAG AGGCCGAAAG CACTTAAGCT TCTGGGAATG 
GAAGATGATG AACCCCCAGC CAAAGGCAAG AAGAAAAAGA AGAAGAAGAA AGAGGAAGAG 
ATTGACATCG ACGTGGACGA CCCCGCCGTG AGTAGGTTCC AGTACCCGTT CCATGAGCTC 
ATGGTATGGG CCGTGCTCAT GAAGCGCCAG AAGATGGCGG TCTTCCTGTG GCAGCGTGGG 
GAAGAGTGCA TGGCGAAGGC CTTGGTGGCC TGCAAGCTCT ACAAGGCCAT GGCCCACGAG 
TCCTCCGAGA GCGAGCTGGT GGATGACATC TCCCAGGATC TGGACAACAA TTCCAAAGAT 
TTTGGCCAGC TCGCTGTGGA GCTGTTAGAC CAGTCTTACA AGCACGATGA GCAGGTTGCC 
ATGAAACTTC TGACCTACGA GCTCAAGAAC TGGAGCAACT CAACCTGCCT GAAGCTGGCT 
GTGGCAGCCA AGCACCGGGA CTTCATTGCT CACACCTGCA GCCAGATGCT GCTGACAGAC 
ATGTGGATGG GAAGGCTTCG CATGAGGAAG AACCCTGGCC TGAAGGTCAT CATGGGGATC 
CTTATCCCAC CCACCATCTT GTTTTTGGAA TTCCGTACAT ATGATGACTT CTCCTATCAA 
ACATCCAAGG AGAACGAAGA TGGCAAAGAA AAGGAAGAAG AGAATGTGGA TGCAAATGCA 
GATGCTGGCT CGAGAAAGGG GGATGAGGAG AATGAGCACA AAAAGCAGAG GAGCATTCCC 
ATTGGAACAA AGATCTGTGA ATTCTATAAT GCGCCCATTG TCAAGTTCTG GTTTTACACA 
ATTTCCTACT TGGGCTACTT GCTGCTGTTC AATTATGTCA TCCTGGTGCG GATGGATGGA 
TGGCCATCAC CCCAAGAATG GATTGTCATC TCCTACATTG TGAGCCTTGC ATTGGAGAAA 
ATACGAGAGA TCCTCATGTC GGAACCAGGC AAACTCAGCC AGAAAATCAA AGTGTGGCTC 
CAGGAGTACT GGAACATCAC AGACCTCGTG GCCATCTCCA TGTTCATGGT TGGGGCCATC 
CTTCGTCTCC AGAGCCAGCC ATACATGGGC TATGGTCGGG TCATCTACTG TGTGGATATC 
ATCCTCTGGT ACATCCGAGT GTTAGACATC TTTGGTGTCA ACAAATATCT TGGTCCCTAT 
GTGATGATGA TTGGAAAGAT GATGATTGAC ATGCTCTACT TTGTGGTCAT TATGCTGGTT 
GTACTGATGA GTTTTGGAGT GGCTCGGCAA GCCATCTTGC ATCCGGAGGA GAAGCCTTCC 
TGGAAACTGG CCCGAAACAT CTTCTACATG CCATACTGGA TGATCTACGG AGAGGTGTTT 
GCAGACCAGA TAGACCTCTA CGCCATGGAA ATCAACCCTC CTTGTGGTGA GAATCTCTAT 
GATGAGGAAG GCAAGAGGCT CCCTCCCTGC ATCCCTGGTG CCTGGCTCAC ACCTGCCCTC 
ATGGCCTGTT ATCTCCTGGT GGCCAATATC CTGCTGGTCA ACCTACTGAT TGCTGTTTTC 
AACAATACCT TCTTTGAAGT CAAGTCAATA TCCAATCAAG TGTGGAAGTT CCAGCGATAC 
CAGCTGATCA TGACATTTCA TGACAGGCCG GTCCTGCCCC CACCAATGAT CATCTTAAGT 
CACATCTATA TCATCATTAT GCGCCTCAGC GGTCGCTGCA GAAAAAAGAG AGAGGGGGAC 
CAGGAGGAGC GGGACCGTGG GTTGAAGTTG TTCCTTAGCG ACGAAGAACT GAAAAAACTG 
CATGAGTTTG AGGAGCAGTG CGTGCAGGAG CACTTCCGGG AAAAGGAGGA TGAGCAGCAG 
TCGTCCAGCG ACGAGCGCAT CCGGGTCACA AGCGAGAGGG TTGAAAATAT GTCAATGAGA 
TTGGAGGAAA TCAACGAAAG AGAAAATTTC ATGAAAACTT CTCTGCAGAC TGTTGACCTT 
CGGCTCTCTC AGCTGGAGGA GCTATCTGGC AGGATGGTGA GCGCTCTGGA GAATCTGGCA 
GGCATCGACA GGTCTGATCT GATCCAGGCT CGATCTCGAG CATCCTCCGA ATGCGAGGCC 
ACCTACCTGC TGCGGCAGAG CAGCATCAAC AGTGCCGACG GCTACAGCTT GTACCGCTAC 
CATTTCAATG GCGAGGAGCT TCTGTTTGAG GAGCCTGCTC TCTCCACTTC GCCAGGGACA 
GCGTTCCGGA AAAAAACCTA CTCCTTTCGT GTGAAGGATG AGGATGCAAA GTCGCACCTG 
GACCAGCCCA GCAACCTGCA CCACACCCCT GGCCCCAGCC CACCTGCCAC GCCAGGCCGC 
AGCCGGCTCG CCCTTGAAGG TCCCTTGAGC ACAGAGCTGA GACCTGGATC GGACCCTGGC 
ATCTCCGCAG GCGAGTTCGA TCCGCGGGCG GATTTCAAGA GCACGGAAGC TGCACCAAGT 
CTGAACGCAG CGGGTGTTAC AGGCACTCAA CTGACTGTCG AAAGCACCGA TTCCCACCCT 
CTGCGGGAGA GCAAACTCGT GCGCTACTAC CCCGGAGACC CTAACACCTA CAAAACAATG 
AAGTCCAGAA GCTTCGTCTA TACTGAAGGA AGAAAGCTAG TCCGAGGTCT TAGCAACTGG 
AGTGCAGAGT ACAGTTCAAT CATGGACCAA GCATGGAACG CGACAGAATG GAGATGTCAA 
GTTCAAAGGA TCACGCGCTC CCGCAGCACA GACATCCCAT ACATCGTGTC CGAAGCAGCC 
TCGCAAGACG AGCTTGAAGA TGAGCACAGA GGATCTCTTC TGGATCCTCA GATCTCCCGT 
TCAGCCCTCA CCGTCTCCGA CAGGCCAGAA AAGGAAAACT TACTCTCTGT GAAGCCACAT 
CAGACTTTAG GATTCCCCTG CCTACGGTCA AGAAGTTTAC ATGGCCGTCC TAGGAGTGCT 
GAGCCCGCTC CTAGCAAATT AGACAGGGCA GGACATGCCA GCAGCACTAG CAACTTAGCA 
GTTATGTCAG TTGTTCCAGA GGGACAAAAC ACCCAGCAAG AGAAAAGAAG CGCGGAAACT 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034193.2
CDS	198..5066
Translation	MGSMRKMSSSFKRGSIKSSTSGSQKGQKAWIEKTFCKRECIFVIPSTKDPNRCCCGQLTNQHIPPLPSGAPSTTGEDTKQADTQSGKWSVSKHTQSYPTDSYGILEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQSFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVVSHVGDALKDHSSKSRGRLCAIGIAPWGMVENKEDLIGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRQLEKHISLQKINTRLGQGVPVVGLVVEGGPNVVSIVLEYLKEDPPVPVVVCDGSGRASDILSFAHKYCDEGGVINESLRDQLLVTIQKTFNYSKSQSYQLFAIIMECMKKKELVTVFRMGSEGQQDVEMAILTALLKGTNASAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPVDTKATEKEKKPPTATTKGRGKGKGKKKGKVKEEVEEETDPRKLELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKSFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEECMAKALVACKLYKAMAHESSESELVDDISQDLDNNSKDFGQLAVELLDQSYKHDEQVAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILIPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENVDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSPQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISMFMVGAILRLQSQPYMGYGRVIYCVDIILWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKKLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERENFMKTSLQTVDLRLSQLEELSGRMVSALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEEPALSTSPGTAFRKKTYSFRVKDEDAKSHLDQPSNLHHTPGPSPPATPGRSRLALEGPLSTELRPGSDPGISAGEFDPRADFKSTEAAPSLNAAGVTGTQLTVESTDSHPLRESKLVRYYPGDPNTYKTMKSRSFVYTEGRKLVRGLSNWSAEYSSIMDQAWNATEWRCQVQRITRSRSTDIPYIVSEAASQDELEDEHRGSLLDPQISRSALTVSDRPEKENLLSVKPHQTLGFPCLRSRSLHGRPRSAEPAPSKLDRAGHASSTSNLAVMSVVPEGQNTQQEKRSAETEC

Target ORF information:

RefSeq Version	NM_001039104.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039104.2

ORF Insert Sequence:

ATGGGGTCCA TGAGGAAGAT GAGCAGCTCC TTCAAGCGTG GTTCAATCAA GAGCTCCACA 
TCAGGGTCCC AGAAGGGTCA GAAAGCATGG ATAGAGAAGA CATTTTGCAA AAGGGAATGC 
ATCTTTGTAA TTCCCAGTAC AAAAGACCCT AACAGATGTT GCTGTGGTCA GCTCACTAAC 
CAGCACATCC CCCCTCTGCC GAGTGGGGCT CCCAGCACAA CAGGAGAGGA CACCAAGCAG 
GCAGACACGC AGTCCGGGAA ATGGTCTGTC AGCAAACACA CCCAGAGCTA CCCAACAGAC 
TCCTATGGGA TTCTTGAATT CCAGGGTGGG GGTTACTCCA ATAAAGCCAT GTACATCCGA 
GTCTCCTACG ACACCAAGCC AGATTCCCTG CTCCACCTCA TGGTGAAGGA CTGGCAGCTG 
GAGCTCCCGA AGCTCTTGAT ATCTGTGCAC GGAGGCCTCC AAAGCTTCGA GATGCAGCCC 
AAACTGAAGC AGGTGTTTGG GAAAGGTCTG ATCAAGGCTG CCATGACCAC GGGGGCGTGG 
ATCTTCACCG GGGGTGTGAG CACTGGTGTC GTCAGCCATG TGGGGGATGC CTTGAAAGAC 
CACTCCTCCA AGTCCAGAGG CCGGCTCTGT GCTATAGGAA TTGCTCCCTG GGGCATGGTG 
GAGAACAAGG AAGACCTGAT TGGAAAAGAT GTAACAAGAG TCTATCAGAC CATGTCCAAC 
CCTCTGAGCA AGCTCTCTGT GCTCAACAAT TCCCACACTC ACTTCATCTT GGCTGACAAC 
GGCACCCTGG GCAAGTATGG TGCTGAGGTG AAGCTTCGAA GACAGCTGGA AAAACACATC 
TCCCTGCAGA AGATCAACAC AAGGCTGGGC CAGGGTGTAC CTGTCGTGGG CCTAGTGGTA 
GAAGGTGGTC CTAACGTGGT TTCTATCGTC CTGGAGTATC TCAAAGAAGA CCCTCCTGTC 
CCTGTGGTGG TTTGCGATGG CAGTGGACGT GCCTCTGACA TTTTGTCCTT CGCACACAAA 
TACTGCGACG AAGGAGGAGT CATAAACGAG TCCCTGCGGG ACCAGCTTCT AGTTACCATT 
CAGAAAACAT TTAATTACAG CAAGTCCCAG TCGTATCAGC TGTTTGCAAT TATCATGGAG 
TGCATGAAGA AGAAAGAACT CGTCACTGTG TTTCGGATGG GTTCCGAGGG TCAGCAAGAT 
GTCGAGATGG CAATTTTAAC TGCCTTGCTC AAAGGAACCA ACGCATCAGC TCCAGATCAG 
CTGAGCTTGG CCCTGGCTTG GAACCGGGTC GACATAGCGC GAAGCCAGAT CTTCGTCTTT 
GGCCCACACT GGCCGCCACT GGGAAGCCTG GCCCCTCCTG TGGACACCAA AGCCACAGAG 
AAGGAAAAGA AGCCACCCAC AGCCACCACC AAGGGGAGAG GAAAAGGAAA AGGCAAGAAG 
AAAGGCAAAG TGAAAGAGGA AGTGGAGGAA GAGACGGACC CCCGGAAGCT TGAGCTGCTC 
AACTGGGTGA ATGCCCTGGA GCAAGCCATG CTGGATGCTC TTGTCCTAGA TCGGGTGGAC 
TTTGTAAAGC TCCTGATTGA AAACGGAGTG AACATGCAGC ATTTCCTCAC CATCCCGAGG 
CTGGAGGAGC TATACAACAC CAGACTGGGC CCACCAAACA CCCTTCATCT GCTGGTGCGG 
GATGTAAAGA AGAGCAACCT TCCACCTGAT TACCACATCA GCCTCATTGA TATAGGACTG 
GTGCTGGAGT ACCTCATGGG CGGTGCCTAC CGCTGCAACT ACACTCGGAA AAGCTTCCGG 
ACTCTCTACA ACAACTTGTT TGGCCCTAAG AGGCCGAAAG CACTTAAGCT TCTGGGAATG 
GAAGATGATG AACCCCCAGC CAAAGGCAAG AAGAAAAAGA AGAAGAAGAA AGAGGAAGAG 
ATTGACATCG ACGTGGACGA CCCCGCCGTG AGTAGGTTCC AGTACCCGTT CCATGAGCTC 
ATGGTATGGG CCGTGCTCAT GAAGCGCCAG AAGATGGCGG TCTTCCTGTG GCAGCGTGGG 
GAAGAGTGCA TGGCGAAGGC CTTGGTGGCC TGCAAGCTCT ACAAGGCCAT GGCCCACGAG 
TCCTCCGAGA GCGAGCTGGT GGATGACATC TCCCAGGATC TGGACAACAA TTCCAAAGAT 
TTTGGCCAGC TCGCTGTGGA GCTGTTAGAC CAGTCTTACA AGCACGATGA GCAGGTTGCC 
ATGAAACTTC TGACCTACGA GCTCAAGAAC TGGAGCAACT CAACCTGCCT GAAGCTGGCT 
GTGGCAGCCA AGCACCGGGA CTTCATTGCT CACACCTGCA GCCAGATGCT GCTGACAGAC 
ATGTGGATGG GAAGGCTTCG CATGAGGAAG AACCCTGGCC TGAAGGTCAT CATGGGGATC 
CTTATCCCAC CCACCATCTT GTTTTTGGAA TTCCGTACAT ATGATGACTT CTCCTATCAA 
ACATCCAAGG AGAACGAAGA TGGCAAAGAA AAGGAAGAAG AGAATGTGGA TGCAAATGCA 
GATGCTGGCT CGAGAAAGGG GGATGAGGAG AATGAGCACA AAAAGCAGAG GAGCATTCCC 
ATTGGAACAA AGATCTGTGA ATTCTATAAT GCGCCCATTG TCAAGTTCTG GTTTTACACA 
ATTTCCTACT TGGGCTACTT GCTGCTGTTC AATTATGTCA TCCTGGTGCG GATGGATGGA 
TGGCCATCAC CCCAAGAATG GATTGTCATC TCCTACATTG TGAGCCTTGC ATTGGAGAAA 
ATACGAGAGA TCCTCATGTC GGAACCAGGC AAACTCAGCC AGAAAATCAA AGTGTGGCTC 
CAGGAGTACT GGAACATCAC AGACCTCGTG GCCATCTCCA TGTTCATGGT TGGGGCCATC 
CTTCGTCTCC AGAGCCAGCC ATACATGGGC TATGGTCGGG TCATCTACTG TGTGGATATC 
ATCCTCTGGT ACATCCGAGT GTTAGACATC TTTGGTGTCA ACAAATATCT TGGTCCCTAT 
GTGATGATGA TTGGAAAGAT GATGATTGAC ATGCTCTACT TTGTGGTCAT TATGCTGGTT 
GTACTGATGA GTTTTGGAGT GGCTCGGCAA GCCATCTTGC ATCCGGAGGA GAAGCCTTCC 
TGGAAACTGG CCCGAAACAT CTTCTACATG CCATACTGGA TGATCTACGG AGAGGTGTTT 
GCAGACCAGA TAGACCTCTA CGCCATGGAA ATCAACCCTC CTTGTGGTGA GAATCTCTAT 
GATGAGGAAG GCAAGAGGCT CCCTCCCTGC ATCCCTGGTG CCTGGCTCAC ACCTGCCCTC 
ATGGCCTGTT ATCTCCTGGT GGCCAATATC CTGCTGGTCA ACCTACTGAT TGCTGTTTTC 
AACAATACCT TCTTTGAAGT CAAGTCAATA TCCAATCAAG TGTGGAAGTT CCAGCGATAC 
CAGCTGATCA TGACATTTCA TGACAGGCCG GTCCTGCCCC CACCAATGAT CATCTTAAGT 
CACATCTATA TCATCATTAT GCGCCTCAGC GGTCGCTGCA GAAAAAAGAG AGAGGGGGAC 
CAGGAGGAGC GGGACCGTGG GTTGAAGTTG TTCCTTAGCG ACGAAGAACT GAAAAAACTG 
CATGAGTTTG AGGAGCAGTG CGTGCAGGAG CACTTCCGGG AAAAGGAGGA TGAGCAGCAG 
TCGTCCAGCG ACGAGCGCAT CCGGGTCACA AGCGAGAGGG TTGAAAATAT GTCAATGAGA 
TTGGAGGAAA TCAACGAAAG AGAAAATTTC ATGAAAACTT CTCTGCAGAC TGTTGACCTT 
CGGCTCTCTC AGCTGGAGGA GCTATCTGGC AGGATGGTGA GCGCTCTGGA GAATCTGGCA 
GGCATCGACA GGTCTGATCT GATCCAGGCT CGATCTCGAG CATCCTCCGA ATGCGAGGCC 
ACCTACCTGC TGCGGCAGAG CAGCATCAAC AGTGCCGACG GCTACAGCTT GTACCGCTAC 
CATTTCAATG GCGAGGAGCT TCTGTTTGAG GAGCCTGCTC TCTCCACTTC GCCAGGGACA 
GCGTTCCGGA AAAAAACCTA CTCCTTTCGT GTGAAGGATG AGGATGCAAA GTCGCACCTG 
GACCAGCCCA GCAACCTGCA CCACACCCCT GGCCCCAGCC CACCTGCCAC GCCAGGCCGC 
AGCCGGCTCG CCCTTGAAGG TCCCTTGAGC ACAGAGCTGA GACCTGGATC GGACCCTGGC 
ATCTCCGCAG GCGAGTTCGA TCCGCGGGCG GATTTCAAGA GCACGGAAGC TGCACCAAGT 
CTGAACGCAG CGGGTGTTAC AGGCACTCAA CTGACTGTCG AAAGCACCGA TTCCCACCCT 
CTGCGGGAGA GCAAACTCGT GCGCTACTAC CCCGGAGACC CTAACACCTA CAAAACAATG 
AAGTCCAGAA GCTTCGTCTA TACTGAAGGA AGAAAGCTAG TCCGAGGTCT TAGCAACTGG 
AGTGCAGAGT ACAGTTCAAT CATGGACCAA GCATGGAACG CGACAGAATG GAGATGTCAA 
GTTCAAAGGA TCACGCGCTC CCGCAGCACA GACATCCCAT ACATCGTGTC CGAAGCAGCC 
TCGCAAGACG AGCTTGAAGA TGAGCACAGA GGATCTCTTC TGGATCCTCA GATCTCCCGT 
TCAGCCCTCA CCGTCTCCGA CAGGCCAGAA AAGGAAAACT TACTCTCTGT GAAGCCACAT 
CAGACTTTAG GATTCCCCTG CCTACGGTCA AGAAGTTTAC ATGGCCGTCC TAGGAGTGCT 
GAGCCCGCTC CTAGCAAATT AGACAGGGCA GGACATGCCA GCAGCACTAG CAACTTAGCA 
GTTATGTCAG TTGTTCCAGA GGGACAAAAC ACCCAGCAAG AGAAAAGAAG CGCGGAAACT 
GAGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The TRPM1 Channel Is Required for Development of the Rod ON Bipolar Cell-AII Amacrine Cell Pathway in the Retinal Circuit.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(41)9889-9900(2017 Oct)
Kozuka T,Chaya T,Tamalu F,Shimada M,Fujimaki-Aoba K,Kuwahara R,Watanabe SI,Furukawa T

TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade.
Proceedings of the National Academy of Sciences of the United States of America107(1)332-7(2010 Jan)
Koike C,Obara T,Uriu Y,Numata T,Sanuki R,Miyata K,Koyasu T,Ueno S,Funabiki K,Tani A,Ueda H,Kondo M,Mori Y,Tachibana M,Furukawa T

Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10.
Development (Cambridge, England)131(20)5139-52(2004 Oct)
Rowan S,Chen CM,Young TL,Fisher DE,Cepko CL

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Trpm1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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