Afdn cDNA ORF clone, Mus musculus(house mouse)

The following Afdn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afdn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu53206 XM_006523752.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53207 XM_006523753.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53208 XM_006523754.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53209 XM_006523755.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53210 XM_006523756.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53211 XM_006523757.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53212 XM_006523758.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53213 XM_006523759.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53214 XM_006523760.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53216 XM_006523762.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53217 XM_006523763.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53218 XM_006523764.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53219 XM_006523765.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53220 XM_006523766.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53221 XM_006523767.3
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu79471 XM_017317275.2
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu79472 XM_017317276.2
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu87108 XM_030249526.1
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu87109 XM_030249527.1
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu87110 XM_030249528.1
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu22404 NM_010806.1
Latest version!
Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
View Old Accession Versions >>
OMu53206 XM_006523752.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53218 XM_006523764.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53216 XM_006523762.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53208 XM_006523754.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53209 XM_006523755.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53214 XM_006523760.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53220 XM_006523766.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53210 XM_006523756.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu79471 XM_017317275.1 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53219 XM_006523765.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53207 XM_006523753.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53211 XM_006523757.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53212 XM_006523758.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu79472 XM_017317276.1 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu53213 XM_006523759.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53217 XM_006523763.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu53221 XM_006523767.2 Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu53206
    Clone ID Related Accession (Same CDS sequence) XM_006523752.2 , XM_006523752.3
    Accession Version XM_006523752.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGTCAGG ATAAGTACCC CAGCACACGG AAGTCTCATG GGCACCTTCC TCTTGCCACC
    CTTAAGCCGC AGCAGCCCCC CTGCCAGCCA CGCCCAGCCT CGGATGGCAT CTCCCTCTGC
    AACTCATTCC AGCCACCCTT GGCCAAAGCC AACAGTACAG CTTACAAAGC AGGCCAGCCC
    CCGCCCCTGC CAGGCAAACC AAGCCTGTAC CGTCCTGGCC ACTGGAAAGG TCGAGGACCA
    AACTCTTACT CGGGATCTGC AGGCACAGCT GTGGGAGCCT ATGATGCCCC TCGGGAAGCG
    AGAGAGAAAC TGACTAGGAG CCAAGACGCA GACTTACCTG GCAGTTCTGG AGCCCCTGAA
    AATCTGACAT TTAAAGAGCG TCAGCGCCTT TTTTCACAAG GGCAGGATGT GTCTGACAAA
    GTGAAAGCTT CTCGTAAATT AACAGAGCTC GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523815.1
    CDS284..6037
    Translation

    Target ORF information:

    RefSeq Version XM_006523752.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523752.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGTCAGG ATAAGTACCC CAGCACACGG AAGTCTCATG GGCACCTTCC TCTTGCCACC
    CTTAAGCCGC AGCAGCCCCC CTGCCAGCCA CGCCCAGCCT CGGATGGCAT CTCCCTCTGC
    AACTCATTCC AGCCACCCTT GGCCAAAGCC AACAGTACAG CTTACAAAGC AGGCCAGCCC
    CCGCCCCTGC CAGGCAAACC AAGCCTGTAC CGTCCTGGCC ACTGGAAAGG TCGAGGACCA
    AACTCTTACT CGGGATCTGC AGGCACAGCT GTGGGAGCCT ATGATGCCCC TCGGGAAGCG
    AGAGAGAAAC TGACTAGGAG CCAAGACGCA GACTTACCTG GCAGTTCTGG AGCCCCTGAA
    AATCTGACAT TTAAAGAGCG TCAGCGCCTT TTTTCACAAG GGCAGGATGT GTCTGACAAA
    GTGAAAGCTT CTCGTAAATT AACAGAGCTC GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53207
    Clone ID Related Accession (Same CDS sequence) XM_006523753.2 , XM_006523753.3
    Accession Version XM_006523753.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523816.1
    CDS178..5928
    Translation

    Target ORF information:

    RefSeq Version XM_006523753.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523753.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53208
    Clone ID Related Accession (Same CDS sequence) XM_006523754.3 , XM_006523754.2
    Accession Version XM_006523754.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523817.1
    CDS178..5928
    Translation

    Target ORF information:

    RefSeq Version XM_006523754.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53209
    Clone ID Related Accession (Same CDS sequence) XM_006523755.3 , XM_006523755.2
    Accession Version XM_006523755.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523755.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523818.1
    CDS284..6034
    Translation

    Target ORF information:

    RefSeq Version XM_006523755.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523755.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53210
    Clone ID Related Accession (Same CDS sequence) XM_006523756.3 , XM_006523756.2
    Accession Version XM_006523756.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523756.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAACATT GCAGGAGCAC TTGCTTTTTG GATGGCAAAT
    GCATCTGAGC TTCTCAACTT CATCAAGCAG GACCGAGACC TTAGTCGGAT CACGCTGGAT
    GCCCAGGATG TCCTAGCACA CTTGGTTCAG ATGGCATTTA AGTACTTGGT TCATTGCCTT
    CAGTCAGAAC TTAATAACTA CATGCCAGCC TTTCTGGATG ATCCTGAGGA GAACAGTCTG
    CAAAGACCAA AAATAGATGA TGTGCTGCAC ACACTCACAG GAGCCATGTC CTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CTTGACCATC CAGCTCTTCT CTCAGCTTTT CCACTTCATC
    AATATGTGGC TGTTCAACAG ACTGGTGACT GACCCAGATT CAGGCCTGTG CTCCCACTAC
    TGGGGTGCCA TTATCCGCCA GCAGCTGGGG CATATTGAAG CGTGGGCAGA GAAGCAGGGG
    CTAGAGCTGG CAGCTGACTG TCACTTGAGC AGAATTGTAC AGGCTACAAC ATTGCTTACG
    ATGGATAAGT ATGTACCTGA TGACATTCCA AATATAAACA GCACATGCTT TAAGTTAAAT
    TCTCTGCAGC TTCAAGCCTT GTTACAAAAT TACCACTGTG CACCCGATGA GCCTTTTATT
    CCTACGGATC TCATAGAAAA TGTTGTGGCT GTTGCTGAGA ACACAGCTGA TGAGCTCGCC
    CGCAGTGATG GCAGAGACGT GCAGCTGGAG GAAGACCCAG ACCTACAGCT GCCTTTTCTT
    CTGCCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGTAGATTGG TTCCTCACAC ACGTTCACCA
    GGTACCTGGA CAATATATTT TGAAGGAGCA GATTATGAAA GTCACCTTAT GCGTGAGAAC
    GCAGAACTGG CCCAGCCTCT GAGGAAGGAG CCTGAGATAA TCACTGTGAC TCTGAAGAAG
    CAGAATGGAA TGGGCCTCAG CATCGTTGCA GCCAAGGGTG CTGGCCAGGA TAAACTGGGA
    ATCTACGTTA AGTCGGTTGT CAAAGGAGGT GCTGCAGATG TGGATGGACG ATTAGCTGCT
    GGTGACCAAC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTTTCTCA GGAAAGGGCA
    GCAGAACTCA TGACAAGAAC CAGTTCCGTG GTTACACTGG AAGTTGCAAA GCAAGGTGCC
    ATCTATCACG GCCTGGCCAC CCTCCTTAAC CAGCCGTCCC CCATGATGCA AAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCAGACCA AAGAGTGAAG GTTTTGAGCT CTATAATAAT
    TCAGCTCAAA ATGGCTCCCC AGAGAGTCCT CAGATGCCTT GGACAGAGTA CAGTGAGCCA
    AAGAAACTGC CTGGGGATGA CAGGCTGATG AAAAACAGAG CGGACCACCG TTCCAGCCCC
    AATGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGGGTC CATATACATC TGGAACAGCA
    GCTAAGATAA CATCTGTCTC CACTGGAAAC CTCTGCACTG AGGAGCAGTC CCCTCCACCC
    AGACCTGAAG CCTATCCTAT CCCTACTCAG ACATACACCA GAGAGTATTT TACCTTTCCA
    GCTTCAAAGT CGCAGGATCG GATGGCTCCT CCTCAGAGCC AGTGGCCAAA TTATGAGGAA
    AAACCACATG TGCACACAGA AAGTAATCAT TCCAGTATTG CAATCCAGCG TGTTACCCGT
    TCCCAGGAAG AGCTTCGGGA AGAAAAGGTT TACCAACTTG AGCGGCATCG AGTAGAGGCA
    GGCATGGATC GCAAGTGTGA CAGTGATATG TGGATCAATC AGAGCTCCTC AGTGGAATCC
    AGCACATCCA GCCAGGAACA CCTGAACCAT TCTTCCAAGT CAGTTACCCC TGCTTCCACT
    CTGACCAAGA GTGGTCCTGG CCGTTGGAAA ACACCAGCAG CGGTGCTGCC CACCCCTGTG
    GCTGTCTCTC AGCCCATTCG AACAGACCTC CCTCCACCAC CACCTCCACC GCCGGTCCAC
    TACACCAGTG AGTTTGATGG TATTCCAATG GATTTGCCCC TCCCACCTCC TCCTGCCAAC
    CAGGCAGGAC CCCAGTCTGC TCAGGTGGCT GCTGCAGAGT GGAAAAAGAG AGAGGAGCAC
    CAGAGGTGGT ATGAGAAAGA GAAGGCCCGC CTGGAGGAAG AACGAGAGAG AAAGCGCAGG
    GAGCAGGAGC GGAAGTTGGG GCAGATGCGC TCTCAGACCC TGAACCCTGC TTCCTTCTCA
    CCTTTGGCCA CACAGGCCAA GCCAGAAAAG CCTTCCACAC TGCAGAGGCC CCAAGAAACA
    GTCATTCGGG AGCTTCAGCC CCAGCAGCAG CCCCGCACGA TCGAGCGCAA GGATCTGCAG
    TATATAACCA TCAGTAAAGA GGAGCTGTCC TCTGGTGATA GTCTGTCTCC AGACCCCTGG
    AAACGAGATG CCAGGGAGAA GCTGGAGAAG CAACAGCAAA TGCACATTGT GGACATGCTA
    AGCAAGGAGA TCCACGAGCT GCAAAACAAG GTGGACCGTA CTGCGGAGGA GAGTGACCGC
    CTGCGGAAGC TTATGCTGGA GTGGCAGTTC CAGAAGAGAC TACAGGAGTC CAAACAGAAG
    GATGAGGATG ATGACGAAGA GGAAGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGT
    CTGGAGGCCG AGCGGAGAGC CAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG
    ATGCAGGATG AGGAGCGCAG GCGCCAGCAA CAGTTGGAAG AGATGCGGAA ACGAGAAGCA
    GAAGATCGAG TAAGACAAGA GGAAGATGGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA
    GATGCTGAAG AAAAGAGGCG ACAGGAGGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG
    CGCCGACAGC ACGAAGAGGC AGCACGCAGG CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC
    CGACCTCCAC TTCCACGGGA CTATGAGCCC CCGTCCTTGT CCTCAGCACC CTGTGCCCCT
    CCTCCCCCAC CTCAGCGAAA CGCATCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG
    CTGTTCACTG CCAAGTTTGT TGCGTATGAT GAGGAGGAGG AGGACTACGG CCCAGCAGGT
    CAGGATAAGT ACCCCAGCAC ACGGAAGTCT CATGGGCACC TTCCTCTTGC CACCCTTAAG
    CCGCAGCAGC CCCCCTGCCA GCCACGCCCA GCCTCGGATG GCATCTCCCT CTGCAACTCA
    TTCCAGCCAC CCTTGGCCAA AGCCAACAGT ACAGCTTACA AAGCAGGCCA GCCCCCGCCC
    CTGCCAGGCA AACCAAGCCT GTACCGTCCT GGCCACTGGA AAGGTCGAGG ACCAAACTCT
    TACTCGGGAT CTGCAGGCAC AGCTGTGGGA GCCTATGATG CCCCTCGGGA AGCGAGAGAG
    AAACTGACTA GGAGCCAAGA CGCAGACTTA CCTGGCAGTT CTGGAGCCCC TGAAAATCTG
    ACATTTAAAG AGCGTCAGCG CCTTTTTTCA CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA
    GCTTCTCGTA AATTAACAGA GCTCGAGAAT GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523819.1
    CDS178..5925
    Translation

    Target ORF information:

    RefSeq Version XM_006523756.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523756.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAACATT GCAGGAGCAC TTGCTTTTTG GATGGCAAAT
    GCATCTGAGC TTCTCAACTT CATCAAGCAG GACCGAGACC TTAGTCGGAT CACGCTGGAT
    GCCCAGGATG TCCTAGCACA CTTGGTTCAG ATGGCATTTA AGTACTTGGT TCATTGCCTT
    CAGTCAGAAC TTAATAACTA CATGCCAGCC TTTCTGGATG ATCCTGAGGA GAACAGTCTG
    CAAAGACCAA AAATAGATGA TGTGCTGCAC ACACTCACAG GAGCCATGTC CTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CTTGACCATC CAGCTCTTCT CTCAGCTTTT CCACTTCATC
    AATATGTGGC TGTTCAACAG ACTGGTGACT GACCCAGATT CAGGCCTGTG CTCCCACTAC
    TGGGGTGCCA TTATCCGCCA GCAGCTGGGG CATATTGAAG CGTGGGCAGA GAAGCAGGGG
    CTAGAGCTGG CAGCTGACTG TCACTTGAGC AGAATTGTAC AGGCTACAAC ATTGCTTACG
    ATGGATAAGT ATGTACCTGA TGACATTCCA AATATAAACA GCACATGCTT TAAGTTAAAT
    TCTCTGCAGC TTCAAGCCTT GTTACAAAAT TACCACTGTG CACCCGATGA GCCTTTTATT
    CCTACGGATC TCATAGAAAA TGTTGTGGCT GTTGCTGAGA ACACAGCTGA TGAGCTCGCC
    CGCAGTGATG GCAGAGACGT GCAGCTGGAG GAAGACCCAG ACCTACAGCT GCCTTTTCTT
    CTGCCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGTAGATTGG TTCCTCACAC ACGTTCACCA
    GGTACCTGGA CAATATATTT TGAAGGAGCA GATTATGAAA GTCACCTTAT GCGTGAGAAC
    GCAGAACTGG CCCAGCCTCT GAGGAAGGAG CCTGAGATAA TCACTGTGAC TCTGAAGAAG
    CAGAATGGAA TGGGCCTCAG CATCGTTGCA GCCAAGGGTG CTGGCCAGGA TAAACTGGGA
    ATCTACGTTA AGTCGGTTGT CAAAGGAGGT GCTGCAGATG TGGATGGACG ATTAGCTGCT
    GGTGACCAAC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTTTCTCA GGAAAGGGCA
    GCAGAACTCA TGACAAGAAC CAGTTCCGTG GTTACACTGG AAGTTGCAAA GCAAGGTGCC
    ATCTATCACG GCCTGGCCAC CCTCCTTAAC CAGCCGTCCC CCATGATGCA AAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCAGACCA AAGAGTGAAG GTTTTGAGCT CTATAATAAT
    TCAGCTCAAA ATGGCTCCCC AGAGAGTCCT CAGATGCCTT GGACAGAGTA CAGTGAGCCA
    AAGAAACTGC CTGGGGATGA CAGGCTGATG AAAAACAGAG CGGACCACCG TTCCAGCCCC
    AATGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGGGTC CATATACATC TGGAACAGCA
    GCTAAGATAA CATCTGTCTC CACTGGAAAC CTCTGCACTG AGGAGCAGTC CCCTCCACCC
    AGACCTGAAG CCTATCCTAT CCCTACTCAG ACATACACCA GAGAGTATTT TACCTTTCCA
    GCTTCAAAGT CGCAGGATCG GATGGCTCCT CCTCAGAGCC AGTGGCCAAA TTATGAGGAA
    AAACCACATG TGCACACAGA AAGTAATCAT TCCAGTATTG CAATCCAGCG TGTTACCCGT
    TCCCAGGAAG AGCTTCGGGA AGAAAAGGTT TACCAACTTG AGCGGCATCG AGTAGAGGCA
    GGCATGGATC GCAAGTGTGA CAGTGATATG TGGATCAATC AGAGCTCCTC AGTGGAATCC
    AGCACATCCA GCCAGGAACA CCTGAACCAT TCTTCCAAGT CAGTTACCCC TGCTTCCACT
    CTGACCAAGA GTGGTCCTGG CCGTTGGAAA ACACCAGCAG CGGTGCTGCC CACCCCTGTG
    GCTGTCTCTC AGCCCATTCG AACAGACCTC CCTCCACCAC CACCTCCACC GCCGGTCCAC
    TACACCAGTG AGTTTGATGG TATTCCAATG GATTTGCCCC TCCCACCTCC TCCTGCCAAC
    CAGGCAGGAC CCCAGTCTGC TCAGGTGGCT GCTGCAGAGT GGAAAAAGAG AGAGGAGCAC
    CAGAGGTGGT ATGAGAAAGA GAAGGCCCGC CTGGAGGAAG AACGAGAGAG AAAGCGCAGG
    GAGCAGGAGC GGAAGTTGGG GCAGATGCGC TCTCAGACCC TGAACCCTGC TTCCTTCTCA
    CCTTTGGCCA CACAGGCCAA GCCAGAAAAG CCTTCCACAC TGCAGAGGCC CCAAGAAACA
    GTCATTCGGG AGCTTCAGCC CCAGCAGCAG CCCCGCACGA TCGAGCGCAA GGATCTGCAG
    TATATAACCA TCAGTAAAGA GGAGCTGTCC TCTGGTGATA GTCTGTCTCC AGACCCCTGG
    AAACGAGATG CCAGGGAGAA GCTGGAGAAG CAACAGCAAA TGCACATTGT GGACATGCTA
    AGCAAGGAGA TCCACGAGCT GCAAAACAAG GTGGACCGTA CTGCGGAGGA GAGTGACCGC
    CTGCGGAAGC TTATGCTGGA GTGGCAGTTC CAGAAGAGAC TACAGGAGTC CAAACAGAAG
    GATGAGGATG ATGACGAAGA GGAAGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGT
    CTGGAGGCCG AGCGGAGAGC CAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG
    ATGCAGGATG AGGAGCGCAG GCGCCAGCAA CAGTTGGAAG AGATGCGGAA ACGAGAAGCA
    GAAGATCGAG TAAGACAAGA GGAAGATGGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA
    GATGCTGAAG AAAAGAGGCG ACAGGAGGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG
    CGCCGACAGC ACGAAGAGGC AGCACGCAGG CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC
    CGACCTCCAC TTCCACGGGA CTATGAGCCC CCGTCCTTGT CCTCAGCACC CTGTGCCCCT
    CCTCCCCCAC CTCAGCGAAA CGCATCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG
    CTGTTCACTG CCAAGTTTGT TGCGTATGAT GAGGAGGAGG AGGACTACGG CCCAGCAGGT
    CAGGATAAGT ACCCCAGCAC ACGGAAGTCT CATGGGCACC TTCCTCTTGC CACCCTTAAG
    CCGCAGCAGC CCCCCTGCCA GCCACGCCCA GCCTCGGATG GCATCTCCCT CTGCAACTCA
    TTCCAGCCAC CCTTGGCCAA AGCCAACAGT ACAGCTTACA AAGCAGGCCA GCCCCCGCCC
    CTGCCAGGCA AACCAAGCCT GTACCGTCCT GGCCACTGGA AAGGTCGAGG ACCAAACTCT
    TACTCGGGAT CTGCAGGCAC AGCTGTGGGA GCCTATGATG CCCCTCGGGA AGCGAGAGAG
    AAACTGACTA GGAGCCAAGA CGCAGACTTA CCTGGCAGTT CTGGAGCCCC TGAAAATCTG
    ACATTTAAAG AGCGTCAGCG CCTTTTTTCA CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA
    GCTTCTCGTA AATTAACAGA GCTCGAGAAT GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53211
    Clone ID Related Accession (Same CDS sequence) XM_006523757.2 , XM_006523757.3
    Accession Version XM_006523757.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523757.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGTCAGGA TAAGTACCCC
    AGCACACGGA AGTCTCATGG GCACCTTCCT CTTGCCACCC TTAAGCCGCA GCAGCCCCCC
    TGCCAGCCAC GCCCAGCCTC GGATGGCATC TCCCTCTGCA ACTCATTCCA GCCACCCTTG
    GCCAAAGCCA ACAGTACAGC TTACAAAGCA GGCCAGCCCC CGCCCCTGCC AGGCAAACCA
    AGCCTGTACC GTCCTGGCCA CTGGAAAGGT CGAGGACCAA ACTCTTACTC GGGATCTGCA
    GGCACAGCTG TGGGAGCCTA TGATGCCCCT CGGGAAGCGA GAGAGAAACT GACTAGGAGC
    CAAGACGCAG ACTTACCTGG CAGTTCTGGA GCCCCTGAAA ATCTGACATT TAAAGAGCGT
    CAGCGCCTTT TTTCACAAGG GCAGGATGTG TCTGACAAAG TGAAAGCTTC TCGTAAATTA
    ACAGAGCTCG AGAATGAACT GAACACAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523820.1
    CDS284..6016
    Translation

    Target ORF information:

    RefSeq Version XM_006523757.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523757.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGTCAGGA TAAGTACCCC
    AGCACACGGA AGTCTCATGG GCACCTTCCT CTTGCCACCC TTAAGCCGCA GCAGCCCCCC
    TGCCAGCCAC GCCCAGCCTC GGATGGCATC TCCCTCTGCA ACTCATTCCA GCCACCCTTG
    GCCAAAGCCA ACAGTACAGC TTACAAAGCA GGCCAGCCCC CGCCCCTGCC AGGCAAACCA
    AGCCTGTACC GTCCTGGCCA CTGGAAAGGT CGAGGACCAA ACTCTTACTC GGGATCTGCA
    GGCACAGCTG TGGGAGCCTA TGATGCCCCT CGGGAAGCGA GAGAGAAACT GACTAGGAGC
    CAAGACGCAG ACTTACCTGG CAGTTCTGGA GCCCCTGAAA ATCTGACATT TAAAGAGCGT
    CAGCGCCTTT TTTCACAAGG GCAGGATGTG TCTGACAAAG TGAAAGCTTC TCGTAAATTA
    ACAGAGCTCG AGAATGAACT GAACACAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53212
    Clone ID Related Accession (Same CDS sequence) XM_006523758.2 , XM_006523758.3
    Accession Version XM_006523758.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523821.1
    CDS178..5907
    Translation

    Target ORF information:

    RefSeq Version XM_006523758.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523758.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53213
    Clone ID Related Accession (Same CDS sequence) XM_006523759.2 , XM_006523759.3
    Accession Version XM_006523759.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGT CAGGATAAGT ACCCCAGCAC ACGGAAGTCT
    CATGGGCACC TTCCTCTTGC CACCCTTAAG CCGCAGCAGC CCCCCTGCCA GCCACGCCCA
    GCCTCGGATG GCATCTCCCT CTGCAACTCA TTCCAGCCAC CCTTGGCCAA AGCCAACAGT
    ACAGCTTACA AAGCAGGCCA GCCCCCGCCC CTGCCAGGCA AACCAAGCCT GTACCGTCCT
    GGCCACTGGA AAGGTCGAGG ACCAAACTCT TACTCGGGAT CTGCAGGCAC AGCTGTGGGA
    GCCTATGATG CCCCTCGGGA AGCGAGAGAG AAACTGACTA GGAGCCAAGA CGCAGACTTA
    CCTGGCAGTT CTGGAGCCCC TGAAAATCTG ACATTTAAAG AGCGTCAGCG CCTTTTTTCA
    CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA GCTTCTCGTA AATTAACAGA GCTCGAGAAT
    GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523822.1
    CDS284..6001
    Translation

    Target ORF information:

    RefSeq Version XM_006523759.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523759.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGT CAGGATAAGT ACCCCAGCAC ACGGAAGTCT
    CATGGGCACC TTCCTCTTGC CACCCTTAAG CCGCAGCAGC CCCCCTGCCA GCCACGCCCA
    GCCTCGGATG GCATCTCCCT CTGCAACTCA TTCCAGCCAC CCTTGGCCAA AGCCAACAGT
    ACAGCTTACA AAGCAGGCCA GCCCCCGCCC CTGCCAGGCA AACCAAGCCT GTACCGTCCT
    GGCCACTGGA AAGGTCGAGG ACCAAACTCT TACTCGGGAT CTGCAGGCAC AGCTGTGGGA
    GCCTATGATG CCCCTCGGGA AGCGAGAGAG AAACTGACTA GGAGCCAAGA CGCAGACTTA
    CCTGGCAGTT CTGGAGCCCC TGAAAATCTG ACATTTAAAG AGCGTCAGCG CCTTTTTTCA
    CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA GCTTCTCGTA AATTAACAGA GCTCGAGAAT
    GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53214
    Clone ID Related Accession (Same CDS sequence) XM_006523760.2 , XM_006523760.3
    Accession Version XM_006523760.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523760.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGAGCA
    CTTGCTTTTT GGATGGCAAA TGCATCTGAG CTTCTCAACT TCATCAAGCA GGACCGAGAC
    CTTAGTCGGA TCACGCTGGA TGCCCAGGAT GTCCTAGCAC ACTTGGTTCA GATGGCATTT
    AAGTACTTGG TTCATTGCCT TCAGTCAGAA CTTAATAACT ACATGCCAGC CTTTCTGGAT
    GATCCTGAGG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACACTCACA
    GGAGCCATGT CCTTGCTGCG GCGCTGCAGA GTCAATGCTG CCTTGACCAT CCAGCTCTTC
    TCTCAGCTTT TCCACTTCAT CAATATGTGG CTGTTCAACA GACTGGTGAC TGACCCAGAT
    TCAGGCCTGT GCTCCCACTA CTGGGGTGCC ATTATCCGCC AGCAGCTGGG GCATATTGAA
    GCGTGGGCAG AGAAGCAGGG GCTAGAGCTG GCAGCTGACT GTCACTTGAG CAGAATTGTA
    CAGGCTACAA CATTGCTTAC GATGGATAAG TATGTACCTG ATGACATTCC AAATATAAAC
    AGCACATGCT TTAAGTTAAA TTCTCTGCAG CTTCAAGCCT TGTTACAAAA TTACCACTGT
    GCACCCGATG AGCCTTTTAT TCCTACGGAT CTCATAGAAA ATGTTGTGGC TGTTGCTGAG
    AACACAGCTG ATGAGCTCGC CCGCAGTGAT GGCAGAGACG TGCAGCTGGA GGAAGACCCA
    GACCTACAGC TGCCTTTTCT TCTGCCAGAA GATGGCTATT CCTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGTAGATTG
    GTTCCTCACA CACGTTCACC AGGTACCTGG ACAATATATT TTGAAGGAGC AGATTATGAA
    AGTCACCTTA TGCGTGAGAA CGCAGAACTG GCCCAGCCTC TGAGGAAGGA GCCTGAGATA
    ATCACTGTGA CTCTGAAGAA GCAGAATGGA ATGGGCCTCA GCATCGTTGC AGCCAAGGGT
    GCTGGCCAGG ATAAACTGGG AATCTACGTT AAGTCGGTTG TCAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GATTAGCTGC TGGTGACCAA CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTTTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CCAGTTCCGT GGTTACACTG
    GAAGTTGCAA AGCAAGGTGC CATCTATCAC GGCCTGGCCA CCCTCCTTAA CCAGCCGTCC
    CCCATGATGC AAAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCAGACC AAAGAGTGAA
    GGTTTTGAGC TCTATAATAA TTCAGCTCAA AATGGCTCCC CAGAGAGTCC TCAGATGCCT
    TGGACAGAGT ACAGTGAGCC AAAGAAACTG CCTGGGGATG ACAGGCTGAT GAAAAACAGA
    GCGGACCACC GTTCCAGCCC CAATGTGGCA AATCAGCCTC CTAGCCCTGG AGGGAAGGGT
    CCATATACAT CTGGAACAGC AGCTAAGATA ACATCTGTCT CCACTGGAAA CCTCTGCACT
    GAGGAGCAGT CCCCTCCACC CAGACCTGAA GCCTATCCTA TCCCTACTCA GACATACACC
    AGAGAGTATT TTACCTTTCC AGCTTCAAAG TCGCAGGATC GGATGGCTCC TCCTCAGAGC
    CAGTGGCCAA ATTATGAGGA AAAACCACAT GTGCACACAG AAAGTAATCA TTCCAGTATT
    GCAATCCAGC GTGTTACCCG TTCCCAGGAA GAGCTTCGGG AAGAAAAGGT TTACCAACTT
    GAGCGGCATC GAGTAGAGGC AGGCATGGAT CGCAAGTGTG ACAGTGATAT GTGGATCAAT
    CAGAGCTCCT CAGTGGAATC CAGCACATCC AGCCAGGAAC ACCTGAACCA TTCTTCCAAG
    TCAGTTACCC CTGCTTCCAC TCTGACCAAG AGTGGTCCTG GCCGTTGGAA AACACCAGCA
    GCGGTGCTGC CCACCCCTGT GGCTGTCTCT CAGCCCATTC GAACAGACCT CCCTCCACCA
    CCACCTCCAC CGCCGGTCCA CTACACCAGT GAGTTTGATG GTATTCCAAT GGATTTGCCC
    CTCCCACCTC CTCCTGCCAA CCAGGCAGGA CCCCAGTCTG CTCAGGTGGC TGCTGCAGAG
    TGGAAAAAGA GAGAGGAGCA CCAGAGGTGG TATGAGAAAG AGAAGGCCCG CCTGGAGGAA
    GAACGAGAGA GAAAGCGCAG GGAGCAGGAG CGGAAGTTGG GGCAGATGCG CTCTCAGACC
    CTGAACCCTG CTTCCTTCTC ACCTTTGGCC ACACAGGCCA AGCCAGAAAA GCCTTCCACA
    CTGCAGAGGC CCCAAGAAAC AGTCATTCGG GAGCTTCAGC CCCAGCAGCA GCCCCGCACG
    ATCGAGCGCA AGGATCTGCA GTATATAACC ATCAGTAAAG AGGAGCTGTC CTCTGGTGAT
    AGTCTGTCTC CAGACCCCTG GAAACGAGAT GCCAGGGAGA AGCTGGAGAA GCAACAGCAA
    ATGCACATTG TGGACATGCT AAGCAAGGAG ATCCACGAGC TGCAAAACAA GGTGGACCGT
    ACTGCGGAGG AGAGTGACCG CCTGCGGAAG CTTATGCTGG AGTGGCAGTT CCAGAAGAGA
    CTACAGGAGT CCAAACAGAA GGATGAGGAT GATGACGAAG AGGAAGATGA TGATGTGGAC
    ACCATGCTGA TCATGCAGCG TCTGGAGGCC GAGCGGAGAG CCAGGCAGAC TGCTATGCCA
    GCAATCTCTG TTCTAGATTT GATGCAGGAT GAGGAGCGCA GGCGCCAGCA ACAGTTGGAA
    GAGATGCGGA AACGAGAAGC AGAAGATCGA GTAAGACAAG AGGAAGATGG ACGCCATCAG
    GAGGAGGAGC GAGTAAAGAG AGATGCTGAA GAAAAGAGGC GACAGGAGGA AGGGTATTAC
    AGCCGCCTAG AAGCTGAGAG GCGCCGACAG CACGAAGAGG CAGCACGCAG GCTGCTGGAG
    CCTGAAGAGC CCGGGCTGAG CCGACCTCCA CTTCCACGGG ACTATGAGCC CCCGTCCTTG
    TCCTCAGCAC CCTGTGCCCC TCCTCCCCCA CCTCAGCGAA ACGCATCCTA CCTCAAAACA
    CAGGTCCTCT CCCCAGACTC GCTGTTCACT GCCAAGTTTG TTGCGTATGA TGAGGAGGAG
    GAGGACTACG GCCCAGCAGG TCAGGATAAG TACCCCAGCA CACGGAAGTC TCATGGGCAC
    CTTCCTCTTG CCACCCTTAA GCCGCAGCAG CCCCCCTGCC AGCCACGCCC AGCCTCGGAT
    GGCATCTCCC TCTGCAACTC ATTCCAGCCA CCCTTGGCCA AAGCCAACAG TACAGCTTAC
    AAAGCAGGCC AGCCCCCGCC CCTGCCAGGC AAACCAAGCC TGTACCGTCC TGGCCACTGG
    AAAGGTCGAG GACCAAACTC TTACTCGGGA TCTGCAGGCA CAGCTGTGGG AGCCTATGAT
    GCCCCTCGGG AAGCGAGAGA GAAACTGACT AGGAGCCAAG ACGCAGACTT ACCTGGCAGT
    TCTGGAGCCC CTGAAAATCT GACATTTAAA GAGCGTCAGC GCCTTTTTTC ACAAGGGCAG
    GATGTGTCTG ACAAAGTGAA AGCTTCTCGT AAATTAACAG AGCTCGAGAA TGAACTGAAC
    ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523823.1
    CDS284..5992
    Translation

    Target ORF information:

    RefSeq Version XM_006523760.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523760.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGAGCA
    CTTGCTTTTT GGATGGCAAA TGCATCTGAG CTTCTCAACT TCATCAAGCA GGACCGAGAC
    CTTAGTCGGA TCACGCTGGA TGCCCAGGAT GTCCTAGCAC ACTTGGTTCA GATGGCATTT
    AAGTACTTGG TTCATTGCCT TCAGTCAGAA CTTAATAACT ACATGCCAGC CTTTCTGGAT
    GATCCTGAGG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACACTCACA
    GGAGCCATGT CCTTGCTGCG GCGCTGCAGA GTCAATGCTG CCTTGACCAT CCAGCTCTTC
    TCTCAGCTTT TCCACTTCAT CAATATGTGG CTGTTCAACA GACTGGTGAC TGACCCAGAT
    TCAGGCCTGT GCTCCCACTA CTGGGGTGCC ATTATCCGCC AGCAGCTGGG GCATATTGAA
    GCGTGGGCAG AGAAGCAGGG GCTAGAGCTG GCAGCTGACT GTCACTTGAG CAGAATTGTA
    CAGGCTACAA CATTGCTTAC GATGGATAAG TATGTACCTG ATGACATTCC AAATATAAAC
    AGCACATGCT TTAAGTTAAA TTCTCTGCAG CTTCAAGCCT TGTTACAAAA TTACCACTGT
    GCACCCGATG AGCCTTTTAT TCCTACGGAT CTCATAGAAA ATGTTGTGGC TGTTGCTGAG
    AACACAGCTG ATGAGCTCGC CCGCAGTGAT GGCAGAGACG TGCAGCTGGA GGAAGACCCA
    GACCTACAGC TGCCTTTTCT TCTGCCAGAA GATGGCTATT CCTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGTAGATTG
    GTTCCTCACA CACGTTCACC AGGTACCTGG ACAATATATT TTGAAGGAGC AGATTATGAA
    AGTCACCTTA TGCGTGAGAA CGCAGAACTG GCCCAGCCTC TGAGGAAGGA GCCTGAGATA
    ATCACTGTGA CTCTGAAGAA GCAGAATGGA ATGGGCCTCA GCATCGTTGC AGCCAAGGGT
    GCTGGCCAGG ATAAACTGGG AATCTACGTT AAGTCGGTTG TCAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GATTAGCTGC TGGTGACCAA CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTTTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CCAGTTCCGT GGTTACACTG
    GAAGTTGCAA AGCAAGGTGC CATCTATCAC GGCCTGGCCA CCCTCCTTAA CCAGCCGTCC
    CCCATGATGC AAAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCAGACC AAAGAGTGAA
    GGTTTTGAGC TCTATAATAA TTCAGCTCAA AATGGCTCCC CAGAGAGTCC TCAGATGCCT
    TGGACAGAGT ACAGTGAGCC AAAGAAACTG CCTGGGGATG ACAGGCTGAT GAAAAACAGA
    GCGGACCACC GTTCCAGCCC CAATGTGGCA AATCAGCCTC CTAGCCCTGG AGGGAAGGGT
    CCATATACAT CTGGAACAGC AGCTAAGATA ACATCTGTCT CCACTGGAAA CCTCTGCACT
    GAGGAGCAGT CCCCTCCACC CAGACCTGAA GCCTATCCTA TCCCTACTCA GACATACACC
    AGAGAGTATT TTACCTTTCC AGCTTCAAAG TCGCAGGATC GGATGGCTCC TCCTCAGAGC
    CAGTGGCCAA ATTATGAGGA AAAACCACAT GTGCACACAG AAAGTAATCA TTCCAGTATT
    GCAATCCAGC GTGTTACCCG TTCCCAGGAA GAGCTTCGGG AAGAAAAGGT TTACCAACTT
    GAGCGGCATC GAGTAGAGGC AGGCATGGAT CGCAAGTGTG ACAGTGATAT GTGGATCAAT
    CAGAGCTCCT CAGTGGAATC CAGCACATCC AGCCAGGAAC ACCTGAACCA TTCTTCCAAG
    TCAGTTACCC CTGCTTCCAC TCTGACCAAG AGTGGTCCTG GCCGTTGGAA AACACCAGCA
    GCGGTGCTGC CCACCCCTGT GGCTGTCTCT CAGCCCATTC GAACAGACCT CCCTCCACCA
    CCACCTCCAC CGCCGGTCCA CTACACCAGT GAGTTTGATG GTATTCCAAT GGATTTGCCC
    CTCCCACCTC CTCCTGCCAA CCAGGCAGGA CCCCAGTCTG CTCAGGTGGC TGCTGCAGAG
    TGGAAAAAGA GAGAGGAGCA CCAGAGGTGG TATGAGAAAG AGAAGGCCCG CCTGGAGGAA
    GAACGAGAGA GAAAGCGCAG GGAGCAGGAG CGGAAGTTGG GGCAGATGCG CTCTCAGACC
    CTGAACCCTG CTTCCTTCTC ACCTTTGGCC ACACAGGCCA AGCCAGAAAA GCCTTCCACA
    CTGCAGAGGC CCCAAGAAAC AGTCATTCGG GAGCTTCAGC CCCAGCAGCA GCCCCGCACG
    ATCGAGCGCA AGGATCTGCA GTATATAACC ATCAGTAAAG AGGAGCTGTC CTCTGGTGAT
    AGTCTGTCTC CAGACCCCTG GAAACGAGAT GCCAGGGAGA AGCTGGAGAA GCAACAGCAA
    ATGCACATTG TGGACATGCT AAGCAAGGAG ATCCACGAGC TGCAAAACAA GGTGGACCGT
    ACTGCGGAGG AGAGTGACCG CCTGCGGAAG CTTATGCTGG AGTGGCAGTT CCAGAAGAGA
    CTACAGGAGT CCAAACAGAA GGATGAGGAT GATGACGAAG AGGAAGATGA TGATGTGGAC
    ACCATGCTGA TCATGCAGCG TCTGGAGGCC GAGCGGAGAG CCAGGCAGAC TGCTATGCCA
    GCAATCTCTG TTCTAGATTT GATGCAGGAT GAGGAGCGCA GGCGCCAGCA ACAGTTGGAA
    GAGATGCGGA AACGAGAAGC AGAAGATCGA GTAAGACAAG AGGAAGATGG ACGCCATCAG
    GAGGAGGAGC GAGTAAAGAG AGATGCTGAA GAAAAGAGGC GACAGGAGGA AGGGTATTAC
    AGCCGCCTAG AAGCTGAGAG GCGCCGACAG CACGAAGAGG CAGCACGCAG GCTGCTGGAG
    CCTGAAGAGC CCGGGCTGAG CCGACCTCCA CTTCCACGGG ACTATGAGCC CCCGTCCTTG
    TCCTCAGCAC CCTGTGCCCC TCCTCCCCCA CCTCAGCGAA ACGCATCCTA CCTCAAAACA
    CAGGTCCTCT CCCCAGACTC GCTGTTCACT GCCAAGTTTG TTGCGTATGA TGAGGAGGAG
    GAGGACTACG GCCCAGCAGG TCAGGATAAG TACCCCAGCA CACGGAAGTC TCATGGGCAC
    CTTCCTCTTG CCACCCTTAA GCCGCAGCAG CCCCCCTGCC AGCCACGCCC AGCCTCGGAT
    GGCATCTCCC TCTGCAACTC ATTCCAGCCA CCCTTGGCCA AAGCCAACAG TACAGCTTAC
    AAAGCAGGCC AGCCCCCGCC CCTGCCAGGC AAACCAAGCC TGTACCGTCC TGGCCACTGG
    AAAGGTCGAG GACCAAACTC TTACTCGGGA TCTGCAGGCA CAGCTGTGGG AGCCTATGAT
    GCCCCTCGGG AAGCGAGAGA GAAACTGACT AGGAGCCAAG ACGCAGACTT ACCTGGCAGT
    TCTGGAGCCC CTGAAAATCT GACATTTAAA GAGCGTCAGC GCCTTTTTTC ACAAGGGCAG
    GATGTGTCTG ACAAAGTGAA AGCTTCTCGT AAATTAACAG AGCTCGAGAA TGAACTGAAC
    ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53216
    Clone ID Related Accession (Same CDS sequence) XM_006523762.2 , XM_006523762.3
    Accession Version XM_006523762.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523762.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGATGCAG
    GATGAGGAGC GCAGGCGCCA GCAACAGTTG GAAGAGATGC GGAAACGAGA AGCAGAAGAT
    CGAGTAAGAC AAGAGGAAGA TGGACGCCAT CAGGAGGAGG AGCGAGTAAA GAGAGATGCT
    GAAGAAAAGA GGCGACAGGA GGAAGGGTAT TACAGCCGCC TAGAAGCTGA GAGGCGCCGA
    CAGCACGAAG AGGCAGCACG CAGGCTGCTG GAGCCTGAAG AGCCCGGGCT GAGCCGACCT
    CCACTTCCAC GGGACTATGA GCCCCCGTCC TTGTCCTCAG CACCCTGTGC CCCTCCTCCC
    CCACCTCAGC GAAACGCATC CTACCTCAAA ACACAGGTCC TCTCCCCAGA CTCGCTGTTC
    ACTGCCAAGT TTGTTGCGTA TGATGAGGAG GAGGAGGACT ACGGCCCAGC AGGTCAGGAT
    AAGTACCCCA GCACACGGAA GTCTCATGGG CACCTTCCTC TTGCCACCCT TAAGCCGCAG
    CAGCCCCCCT GCCAGCCACG CCCAGCCTCG GATGGCATCT CCCTCTGCAA CTCATTCCAG
    CCACCCTTGG CCAAAGCCAA CAGTACAGCT TACAAAGCAG GCCAGCCCCC GCCCCTGCCA
    GGCAAACCAA GCCTGTACCG TCCTGGCCAC TGGAAAGGTC GAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523825.1
    CDS178..5859
    Translation

    Target ORF information:

    RefSeq Version XM_006523762.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523762.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGATGCAG
    GATGAGGAGC GCAGGCGCCA GCAACAGTTG GAAGAGATGC GGAAACGAGA AGCAGAAGAT
    CGAGTAAGAC AAGAGGAAGA TGGACGCCAT CAGGAGGAGG AGCGAGTAAA GAGAGATGCT
    GAAGAAAAGA GGCGACAGGA GGAAGGGTAT TACAGCCGCC TAGAAGCTGA GAGGCGCCGA
    CAGCACGAAG AGGCAGCACG CAGGCTGCTG GAGCCTGAAG AGCCCGGGCT GAGCCGACCT
    CCACTTCCAC GGGACTATGA GCCCCCGTCC TTGTCCTCAG CACCCTGTGC CCCTCCTCCC
    CCACCTCAGC GAAACGCATC CTACCTCAAA ACACAGGTCC TCTCCCCAGA CTCGCTGTTC
    ACTGCCAAGT TTGTTGCGTA TGATGAGGAG GAGGAGGACT ACGGCCCAGC AGGTCAGGAT
    AAGTACCCCA GCACACGGAA GTCTCATGGG CACCTTCCTC TTGCCACCCT TAAGCCGCAG
    CAGCCCCCCT GCCAGCCACG CCCAGCCTCG GATGGCATCT CCCTCTGCAA CTCATTCCAG
    CCACCCTTGG CCAAAGCCAA CAGTACAGCT TACAAAGCAG GCCAGCCCCC GCCCCTGCCA
    GGCAAACCAA GCCTGTACCG TCCTGGCCAC TGGAAAGGTC GAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53217
    Clone ID Related Accession (Same CDS sequence) XM_006523763.2 , XM_006523763.3
    Accession Version XM_006523763.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523763.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGACCAA ACTCTTACTC GGGATCTGCA GGCACAGCTG TGGGAGCCTA TGATGCCCCT
    CGGGAAGCGA GAGAGAAACT GACTAGGAGC CAAGACGCAG ACTTACCTGG CAGTTCTGGA
    GCCCCTGAAA ATCTGACATT TAAAGAGCGT CAGCGCCTTT TTTCACAAGG GCAGGATGTG
    TCTGACAAAG TGAAAGCTTC TCGTAAATTA ACAGAGCTCG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523826.1
    CDS284..5806
    Translation

    Target ORF information:

    RefSeq Version XM_006523763.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523763.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGACCAA ACTCTTACTC GGGATCTGCA GGCACAGCTG TGGGAGCCTA TGATGCCCCT
    CGGGAAGCGA GAGAGAAACT GACTAGGAGC CAAGACGCAG ACTTACCTGG CAGTTCTGGA
    GCCCCTGAAA ATCTGACATT TAAAGAGCGT CAGCGCCTTT TTTCACAAGG GCAGGATGTG
    TCTGACAAAG TGAAAGCTTC TCGTAAATTA ACAGAGCTCG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53218
    Clone ID Related Accession (Same CDS sequence) XM_006523764.3 , XM_006523764.2
    Accession Version XM_006523764.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523827.1
    CDS284..5770
    Translation

    Target ORF information:

    RefSeq Version XM_006523764.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523764.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53219
    Clone ID Related Accession (Same CDS sequence) XM_006523765.3 , XM_006523765.2
    Accession Version XM_006523765.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGATG CAGGATGAGG AGCGCAGGCG CCAGCAACAG
    TTGGAAGAGA TGCGGAAACG AGAAGCAGAA GATCGAGTAA GACAAGAGGA AGATGGACGC
    CATCAGGAGG AGGAGCGAGT AAAGAGAGAT GCTGAAGAAA AGAGGCGACA GGAGGAAGGG
    TATTACAGCC GCCTAGAAGC TGAGAGGCGC CGACAGCACG AAGAGGCAGC ACGCAGGCTG
    CTGGAGCCTG AAGAGCCCGG GCTGAGCCGA CCTCCACTTC CACGGGACTA TGAGCCCCCG
    TCCTTGTCCT CAGCACCCTG TGCCCCTCCT CCCCCACCTC AGCGAAACGC ATCCTACCTC
    AAAACACAGG TCCTCTCCCC AGACTCGCTG TTCACTGCCA AGTTTGTTGC GTATGATGAG
    GAGGAGGAGG ACTACGGCCC AGCAGGACCA AACTCTTACT CGGGATCTGC AGGCACAGCT
    GTGGGAGCCT ATGATGCCCC TCGGGAAGCG AGAGAGAAAC TGACTAGGAG CCAAGACGCA
    GACTTACCTG GCAGTTCTGG AGCCCCTGAA AATCTGACAT TTAAAGAGCG TCAGCGCCTT
    TTTTCACAAG GGCAGGATGT GTCTGACAAA GTGAAAGCTT CTCGTAAATT AACAGAGCTC
    GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523828.1
    CDS178..5661
    Translation

    Target ORF information:

    RefSeq Version XM_006523765.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523765.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGATG CAGGATGAGG AGCGCAGGCG CCAGCAACAG
    TTGGAAGAGA TGCGGAAACG AGAAGCAGAA GATCGAGTAA GACAAGAGGA AGATGGACGC
    CATCAGGAGG AGGAGCGAGT AAAGAGAGAT GCTGAAGAAA AGAGGCGACA GGAGGAAGGG
    TATTACAGCC GCCTAGAAGC TGAGAGGCGC CGACAGCACG AAGAGGCAGC ACGCAGGCTG
    CTGGAGCCTG AAGAGCCCGG GCTGAGCCGA CCTCCACTTC CACGGGACTA TGAGCCCCCG
    TCCTTGTCCT CAGCACCCTG TGCCCCTCCT CCCCCACCTC AGCGAAACGC ATCCTACCTC
    AAAACACAGG TCCTCTCCCC AGACTCGCTG TTCACTGCCA AGTTTGTTGC GTATGATGAG
    GAGGAGGAGG ACTACGGCCC AGCAGGACCA AACTCTTACT CGGGATCTGC AGGCACAGCT
    GTGGGAGCCT ATGATGCCCC TCGGGAAGCG AGAGAGAAAC TGACTAGGAG CCAAGACGCA
    GACTTACCTG GCAGTTCTGG AGCCCCTGAA AATCTGACAT TTAAAGAGCG TCAGCGCCTT
    TTTTCACAAG GGCAGGATGT GTCTGACAAA GTGAAAGCTT CTCGTAAATT AACAGAGCTC
    GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53220
    Clone ID Related Accession (Same CDS sequence) XM_006523766.2 , XM_006523766.3
    Accession Version XM_006523766.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523829.1
    CDS284..5785
    Translation

    Target ORF information:

    RefSeq Version XM_006523766.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523766.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53221
    Clone ID Related Accession (Same CDS sequence) XM_006523767.2 , XM_006523767.3
    Accession Version XM_006523767.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGAT GCAGGATGAG GAGCGCAGGC GCCAGCAACA GTTGGAAGAG
    ATGCGGAAAC GAGAAGCAGA AGATCGAGTA AGACAAGAGG AAGATGGACG CCATCAGGAG
    GAGGAGCGAG TAAAGAGAGA TGCTGAAGAA AAGAGGCGAC AGGAGGAAGG GTATTACAGC
    CGCCTAGAAG CTGAGAGGCG CCGACAGCAC GAAGAGGCAG CACGCAGGCT GCTGGAGCCT
    GAAGAGCCCG GGCTGAGCCG ACCTCCACTT CCACGGGACT ATGAGCCCCC GTCCTTGTCC
    TCAGCACCCT GTGCCCCTCC TCCCCCACCT CAGCGAAACG CATCCTACCT CAAAACACAG
    GTCCTCTCCC CAGACTCGCT GTTCACTGCC AAGTTTGTTG CGTATGATGA GGAGGAGGAG
    GACTACGGCC CAGCAGGTCA GGATAAGTAC CCCAGCACAC GGAAGTCTCA TGGGCACCTT
    CCTCTTGCCA CCCTTAAGCC GCAGCAGCCC CCCTGCCAGC CACGCCCAGC CTCGGATGGC
    ATCTCCCTCT GCAACTCATT CCAGCCACCC TTGGCCAAAG CCAACAGTAC AGCTTACAAA
    GCAGGCCAGC CCCCGCCCCT GCCAGGCAAA CCAAGCCTGT ACCGTCCTGG CCACTGGAAA
    GGTCGAGGAC CAAACTCTTA CTCGGGATCT GCAGGCACAG CTGTGGGAGC CTATGATGCC
    CCTCGGGAAG CGAGAGAGAA ACTGACTAGG AGCCAAGACG CAGACTTACC TGGCAGTTCT
    GGAGCCCCTG AAAATCTGAC ATTTAAAGAG CGTCAGCGCC TTTTTTCACA AGGGCAGGAT
    GTGTCTGACA AAGTGAAAGC TTCTCGTAAA TTAACAGAGC TCGAGAATGA ACTGAACACA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523830.1
    CDS178..5823
    Translation

    Target ORF information:

    RefSeq Version XM_006523767.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523767.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGAT GCAGGATGAG GAGCGCAGGC GCCAGCAACA GTTGGAAGAG
    ATGCGGAAAC GAGAAGCAGA AGATCGAGTA AGACAAGAGG AAGATGGACG CCATCAGGAG
    GAGGAGCGAG TAAAGAGAGA TGCTGAAGAA AAGAGGCGAC AGGAGGAAGG GTATTACAGC
    CGCCTAGAAG CTGAGAGGCG CCGACAGCAC GAAGAGGCAG CACGCAGGCT GCTGGAGCCT
    GAAGAGCCCG GGCTGAGCCG ACCTCCACTT CCACGGGACT ATGAGCCCCC GTCCTTGTCC
    TCAGCACCCT GTGCCCCTCC TCCCCCACCT CAGCGAAACG CATCCTACCT CAAAACACAG
    GTCCTCTCCC CAGACTCGCT GTTCACTGCC AAGTTTGTTG CGTATGATGA GGAGGAGGAG
    GACTACGGCC CAGCAGGTCA GGATAAGTAC CCCAGCACAC GGAAGTCTCA TGGGCACCTT
    CCTCTTGCCA CCCTTAAGCC GCAGCAGCCC CCCTGCCAGC CACGCCCAGC CTCGGATGGC
    ATCTCCCTCT GCAACTCATT CCAGCCACCC TTGGCCAAAG CCAACAGTAC AGCTTACAAA
    GCAGGCCAGC CCCCGCCCCT GCCAGGCAAA CCAAGCCTGT ACCGTCCTGG CCACTGGAAA
    GGTCGAGGAC CAAACTCTTA CTCGGGATCT GCAGGCACAG CTGTGGGAGC CTATGATGCC
    CCTCGGGAAG CGAGAGAGAA ACTGACTAGG AGCCAAGACG CAGACTTACC TGGCAGTTCT
    GGAGCCCCTG AAAATCTGAC ATTTAAAGAG CGTCAGCGCC TTTTTTCACA AGGGCAGGAT
    GTGTCTGACA AAGTGAAAGC TTCTCGTAAA TTAACAGAGC TCGAGAATGA ACTGAACACA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79471
    Clone ID Related Accession (Same CDS sequence) XM_017317275.2 , XM_017317275.1
    Accession Version XM_017317275.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172764.1
    CDS178..5907
    Translation

    Target ORF information:

    RefSeq Version XM_017317275.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317275.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79471
    Clone ID Related Accession (Same CDS sequence) XM_017317275.2 , XM_017317275.1
    Accession Version XM_017317275.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172764.1
    CDS131..5860
    Translation

    Target ORF information:

    RefSeq Version XM_017317275.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79472
    Clone ID Related Accession (Same CDS sequence) XM_017317276.1 , XM_017317276.2
    Accession Version XM_017317276.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGAAACAG
    AAGAACATTG CAGGAGCACT TGCTTTTTGG ATGGCAAATG CATCTGAGCT TCTCAACTTC
    ATCAAGCAGG ACCGAGACCT TAGTCGGATC ACGCTGGATG CCCAGGATGT CCTAGCACAC
    TTGGTTCAGA TGGCATTTAA GTACTTGGTT CATTGCCTTC AGTCAGAACT TAATAACTAC
    ATGCCAGCCT TTCTGGATGA TCCTGAGGAG AACAGTCTGC AAAGACCAAA AATAGATGAT
    GTGCTGCACA CACTCACAGG AGCCATGTCC TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    TTGACCATCC AGCTCTTCTC TCAGCTTTTC CACTTCATCA ATATGTGGCT GTTCAACAGA
    CTGGTGACTG ACCCAGATTC AGGCCTGTGC TCCCACTACT GGGGTGCCAT TATCCGCCAG
    CAGCTGGGGC ATATTGAAGC GTGGGCAGAG AAGCAGGGGC TAGAGCTGGC AGCTGACTGT
    CACTTGAGCA GAATTGTACA GGCTACAACA TTGCTTACGA TGGATAAGTA TGTACCTGAT
    GACATTCCAA ATATAAACAG CACATGCTTT AAGTTAAATT CTCTGCAGCT TCAAGCCTTG
    TTACAAAATT ACCACTGTGC ACCCGATGAG CCTTTTATTC CTACGGATCT CATAGAAAAT
    GTTGTGGCTG TTGCTGAGAA CACAGCTGAT GAGCTCGCCC GCAGTGATGG CAGAGACGTG
    CAGCTGGAGG AAGACCCAGA CCTACAGCTG CCTTTTCTTC TGCCAGAAGA TGGCTATTCC
    TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG
    AGAGGATTTT GTAGATTGGT TCCTCACACA CGTTCACCAG GTACCTGGAC AATATATTTT
    GAAGGAGCAG ATTATGAAAG TCACCTTATG CGTGAGAACG CAGAACTGGC CCAGCCTCTG
    AGGAAGGAGC CTGAGATAAT CACTGTGACT CTGAAGAAGC AGAATGGAAT GGGCCTCAGC
    ATCGTTGCAG CCAAGGGTGC TGGCCAGGAT AAACTGGGAA TCTACGTTAA GTCGGTTGTC
    AAAGGAGGTG CTGCAGATGT GGATGGACGA TTAGCTGCTG GTGACCAACT CCTCAGTGTG
    GATGGACGAA GTCTGGTTGG ACTTTCTCAG GAAAGGGCAG CAGAACTCAT GACAAGAACC
    AGTTCCGTGG TTACACTGGA AGTTGCAAAG CAAGGTGCCA TCTATCACGG CCTGGCCACC
    CTCCTTAACC AGCCGTCCCC CATGATGCAA AGAATTTCAG ATCGTCGTGG CTCAGGTAAA
    CCCAGACCAA AGAGTGAAGG TTTTGAGCTC TATAATAATT CAGCTCAAAA TGGCTCCCCA
    GAGAGTCCTC AGATGCCTTG GACAGAGTAC AGTGAGCCAA AGAAACTGCC TGGGGATGAC
    AGGCTGATGA AAAACAGAGC GGACCACCGT TCCAGCCCCA ATGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAGGGTCC ATATACATCT GGAACAGCAG CTAAGATAAC ATCTGTCTCC
    ACTGGAAACC TCTGCACTGA GGAGCAGTCC CCTCCACCCA GACCTGAAGC CTATCCTATC
    CCTACTCAGA CATACACCAG AGAGTATTTT ACCTTTCCAG CTTCAAAGTC GCAGGATCGG
    ATGGCTCCTC CTCAGAGCCA GTGGCCAAAT TATGAGGAAA AACCACATGT GCACACAGAA
    AGTAATCATT CCAGTATTGC AATCCAGCGT GTTACCCGTT CCCAGGAAGA GCTTCGGGAA
    GAAAAGGTTT ACCAACTTGA GCGGCATCGA GTAGAGGCAG GCATGGATCG CAAGTGTGAC
    AGTGATATGT GGATCAATCA GAGCTCCTCA GTGGAATCCA GCACATCCAG CCAGGAACAC
    CTGAACCATT CTTCCAAGTC AGTTACCCCT GCTTCCACTC TGACCAAGAG TGGTCCTGGC
    CGTTGGAAAA CACCAGCAGC GGTGCTGCCC ACCCCTGTGG CTGTCTCTCA GCCCATTCGA
    ACAGACCTCC CTCCACCACC ACCTCCACCG CCGGTCCACT ACACCAGTGA GTTTGATGGT
    ATTCCAATGG ATTTGCCCCT CCCACCTCCT CCTGCCAACC AGGCAGGACC CCAGTCTGCT
    CAGGTGGCTG CTGCAGAGTG GAAAAAGAGA GAGGAGCACC AGAGGTGGTA TGAGAAAGAG
    AAGGCCCGCC TGGAGGAAGA ACGAGAGAGA AAGCGCAGGG AGCAGGAGCG GAAGTTGGGG
    CAGATGCGCT CTCAGACCCT GAACCCTGCT TCCTTCTCAC CTTTGGCCAC ACAGGCCAAG
    CCAGAAAAGC CTTCCACACT GCAGAGGCCC CAAGAAACAG TCATTCGGGA GCTTCAGCCC
    CAGCAGCAGC CCCGCACGAT CGAGCGCAAG GATCTGCAGT ATATAACCAT CAGTAAAGAG
    GAGCTGTCCT CTGGTGATAG TCTGTCTCCA GACCCCTGGA AACGAGATGC CAGGGAGAAG
    CTGGAGAAGC AACAGCAAAT GCACATTGTG GACATGCTAA GCAAGGAGAT CCACGAGCTG
    CAAAACAAGG TGGACCGTAC TGCGGAGGAG AGTGACCGCC TGCGGAAGCT TATGCTGGAG
    TGGCAGTTCC AGAAGAGACT ACAGGAGTCC AAACAGAAGG ATGAGGATGA TGACGAAGAG
    GAAGATGATG ATGTGGACAC CATGCTGATC ATGCAGCGTC TGGAGGCCGA GCGGAGAGCC
    AGGCAGACTG CTATGCCAGC AATCTCTGTT CTAGATTTGA TGCAGGATGA GGAGCGCAGG
    CGCCAGCAAC AGTTGGAAGA GATGCGGAAA CGAGAAGCAG AAGATCGAGT AAGACAAGAG
    GAAGATGGAC GCCATCAGGA GGAGGAGCGA GTAAAGAGAG ATGCTGAAGA AAAGAGGCGA
    CAGGAGGAAG GGTATTACAG CCGCCTAGAA GCTGAGAGGC GCCGACAGCA CGAAGAGGCA
    GCACGCAGGC TGCTGGAGCC TGAAGAGCCC GGGCTGAGCC GACCTCCACT TCCACGGGAC
    TATGAGCCCC CGTCCTTGTC CTCAGCACCC TGTGCCCCTC CTCCCCCACC TCAGCGAAAC
    GCATCCTACC TCAAAACACA GGTCCTCTCC CCAGACTCGC TGTTCACTGC CAAGTTTGTT
    GCGTATGATG AGGAGGAGGA GGACTACGGC CCAGCAGGTC AGGATAAGTA CCCCAGCACA
    CGGAAGTCTC ATGGGCACCT TCCTCTTGCC ACCCTTAAGC CGCAGCAGCC CCCCTGCCAG
    CCACGCCCAG CCTCGGATGG CATCTCCCTC TGCAACTCAT TCCAGCCACC CTTGGCCAAA
    GCCAACAGTA CAGCTTACAA AGCAGGCCAG CCCCCGCCCC TGCCAGGCAA ACCAAGCCTG
    TACCGTCCTG GCCACTGGAA AGGTCGAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172765.1
    CDS131..5857
    Translation

    Target ORF information:

    RefSeq Version XM_017317276.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGAAACAG
    AAGAACATTG CAGGAGCACT TGCTTTTTGG ATGGCAAATG CATCTGAGCT TCTCAACTTC
    ATCAAGCAGG ACCGAGACCT TAGTCGGATC ACGCTGGATG CCCAGGATGT CCTAGCACAC
    TTGGTTCAGA TGGCATTTAA GTACTTGGTT CATTGCCTTC AGTCAGAACT TAATAACTAC
    ATGCCAGCCT TTCTGGATGA TCCTGAGGAG AACAGTCTGC AAAGACCAAA AATAGATGAT
    GTGCTGCACA CACTCACAGG AGCCATGTCC TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    TTGACCATCC AGCTCTTCTC TCAGCTTTTC CACTTCATCA ATATGTGGCT GTTCAACAGA
    CTGGTGACTG ACCCAGATTC AGGCCTGTGC TCCCACTACT GGGGTGCCAT TATCCGCCAG
    CAGCTGGGGC ATATTGAAGC GTGGGCAGAG AAGCAGGGGC TAGAGCTGGC AGCTGACTGT
    CACTTGAGCA GAATTGTACA GGCTACAACA TTGCTTACGA TGGATAAGTA TGTACCTGAT
    GACATTCCAA ATATAAACAG CACATGCTTT AAGTTAAATT CTCTGCAGCT TCAAGCCTTG
    TTACAAAATT ACCACTGTGC ACCCGATGAG CCTTTTATTC CTACGGATCT CATAGAAAAT
    GTTGTGGCTG TTGCTGAGAA CACAGCTGAT GAGCTCGCCC GCAGTGATGG CAGAGACGTG
    CAGCTGGAGG AAGACCCAGA CCTACAGCTG CCTTTTCTTC TGCCAGAAGA TGGCTATTCC
    TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG
    AGAGGATTTT GTAGATTGGT TCCTCACACA CGTTCACCAG GTACCTGGAC AATATATTTT
    GAAGGAGCAG ATTATGAAAG TCACCTTATG CGTGAGAACG CAGAACTGGC CCAGCCTCTG
    AGGAAGGAGC CTGAGATAAT CACTGTGACT CTGAAGAAGC AGAATGGAAT GGGCCTCAGC
    ATCGTTGCAG CCAAGGGTGC TGGCCAGGAT AAACTGGGAA TCTACGTTAA GTCGGTTGTC
    AAAGGAGGTG CTGCAGATGT GGATGGACGA TTAGCTGCTG GTGACCAACT CCTCAGTGTG
    GATGGACGAA GTCTGGTTGG ACTTTCTCAG GAAAGGGCAG CAGAACTCAT GACAAGAACC
    AGTTCCGTGG TTACACTGGA AGTTGCAAAG CAAGGTGCCA TCTATCACGG CCTGGCCACC
    CTCCTTAACC AGCCGTCCCC CATGATGCAA AGAATTTCAG ATCGTCGTGG CTCAGGTAAA
    CCCAGACCAA AGAGTGAAGG TTTTGAGCTC TATAATAATT CAGCTCAAAA TGGCTCCCCA
    GAGAGTCCTC AGATGCCTTG GACAGAGTAC AGTGAGCCAA AGAAACTGCC TGGGGATGAC
    AGGCTGATGA AAAACAGAGC GGACCACCGT TCCAGCCCCA ATGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAGGGTCC ATATACATCT GGAACAGCAG CTAAGATAAC ATCTGTCTCC
    ACTGGAAACC TCTGCACTGA GGAGCAGTCC CCTCCACCCA GACCTGAAGC CTATCCTATC
    CCTACTCAGA CATACACCAG AGAGTATTTT ACCTTTCCAG CTTCAAAGTC GCAGGATCGG
    ATGGCTCCTC CTCAGAGCCA GTGGCCAAAT TATGAGGAAA AACCACATGT GCACACAGAA
    AGTAATCATT CCAGTATTGC AATCCAGCGT GTTACCCGTT CCCAGGAAGA GCTTCGGGAA
    GAAAAGGTTT ACCAACTTGA GCGGCATCGA GTAGAGGCAG GCATGGATCG CAAGTGTGAC
    AGTGATATGT GGATCAATCA GAGCTCCTCA GTGGAATCCA GCACATCCAG CCAGGAACAC
    CTGAACCATT CTTCCAAGTC AGTTACCCCT GCTTCCACTC TGACCAAGAG TGGTCCTGGC
    CGTTGGAAAA CACCAGCAGC GGTGCTGCCC ACCCCTGTGG CTGTCTCTCA GCCCATTCGA
    ACAGACCTCC CTCCACCACC ACCTCCACCG CCGGTCCACT ACACCAGTGA GTTTGATGGT
    ATTCCAATGG ATTTGCCCCT CCCACCTCCT CCTGCCAACC AGGCAGGACC CCAGTCTGCT
    CAGGTGGCTG CTGCAGAGTG GAAAAAGAGA GAGGAGCACC AGAGGTGGTA TGAGAAAGAG
    AAGGCCCGCC TGGAGGAAGA ACGAGAGAGA AAGCGCAGGG AGCAGGAGCG GAAGTTGGGG
    CAGATGCGCT CTCAGACCCT GAACCCTGCT TCCTTCTCAC CTTTGGCCAC ACAGGCCAAG
    CCAGAAAAGC CTTCCACACT GCAGAGGCCC CAAGAAACAG TCATTCGGGA GCTTCAGCCC
    CAGCAGCAGC CCCGCACGAT CGAGCGCAAG GATCTGCAGT ATATAACCAT CAGTAAAGAG
    GAGCTGTCCT CTGGTGATAG TCTGTCTCCA GACCCCTGGA AACGAGATGC CAGGGAGAAG
    CTGGAGAAGC AACAGCAAAT GCACATTGTG GACATGCTAA GCAAGGAGAT CCACGAGCTG
    CAAAACAAGG TGGACCGTAC TGCGGAGGAG AGTGACCGCC TGCGGAAGCT TATGCTGGAG
    TGGCAGTTCC AGAAGAGACT ACAGGAGTCC AAACAGAAGG ATGAGGATGA TGACGAAGAG
    GAAGATGATG ATGTGGACAC CATGCTGATC ATGCAGCGTC TGGAGGCCGA GCGGAGAGCC
    AGGCAGACTG CTATGCCAGC AATCTCTGTT CTAGATTTGA TGCAGGATGA GGAGCGCAGG
    CGCCAGCAAC AGTTGGAAGA GATGCGGAAA CGAGAAGCAG AAGATCGAGT AAGACAAGAG
    GAAGATGGAC GCCATCAGGA GGAGGAGCGA GTAAAGAGAG ATGCTGAAGA AAAGAGGCGA
    CAGGAGGAAG GGTATTACAG CCGCCTAGAA GCTGAGAGGC GCCGACAGCA CGAAGAGGCA
    GCACGCAGGC TGCTGGAGCC TGAAGAGCCC GGGCTGAGCC GACCTCCACT TCCACGGGAC
    TATGAGCCCC CGTCCTTGTC CTCAGCACCC TGTGCCCCTC CTCCCCCACC TCAGCGAAAC
    GCATCCTACC TCAAAACACA GGTCCTCTCC CCAGACTCGC TGTTCACTGC CAAGTTTGTT
    GCGTATGATG AGGAGGAGGA GGACTACGGC CCAGCAGGTC AGGATAAGTA CCCCAGCACA
    CGGAAGTCTC ATGGGCACCT TCCTCTTGCC ACCCTTAAGC CGCAGCAGCC CCCCTGCCAG
    CCACGCCCAG CCTCGGATGG CATCTCCCTC TGCAACTCAT TCCAGCCACC CTTGGCCAAA
    GCCAACAGTA CAGCTTACAA AGCAGGCCAG CCCCCGCCCC TGCCAGGCAA ACCAAGCCTG
    TACCGTCCTG GCCACTGGAA AGGTCGAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79472
    Clone ID Related Accession (Same CDS sequence) XM_017317276.1 , XM_017317276.2
    Accession Version XM_017317276.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGAAACAG
    AAGAACATTG CAGGAGCACT TGCTTTTTGG ATGGCAAATG CATCTGAGCT TCTCAACTTC
    ATCAAGCAGG ACCGAGACCT TAGTCGGATC ACGCTGGATG CCCAGGATGT CCTAGCACAC
    TTGGTTCAGA TGGCATTTAA GTACTTGGTT CATTGCCTTC AGTCAGAACT TAATAACTAC
    ATGCCAGCCT TTCTGGATGA TCCTGAGGAG AACAGTCTGC AAAGACCAAA AATAGATGAT
    GTGCTGCACA CACTCACAGG AGCCATGTCC TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    TTGACCATCC AGCTCTTCTC TCAGCTTTTC CACTTCATCA ATATGTGGCT GTTCAACAGA
    CTGGTGACTG ACCCAGATTC AGGCCTGTGC TCCCACTACT GGGGTGCCAT TATCCGCCAG
    CAGCTGGGGC ATATTGAAGC GTGGGCAGAG AAGCAGGGGC TAGAGCTGGC AGCTGACTGT
    CACTTGAGCA GAATTGTACA GGCTACAACA TTGCTTACGA TGGATAAGTA TGTACCTGAT
    GACATTCCAA ATATAAACAG CACATGCTTT AAGTTAAATT CTCTGCAGCT TCAAGCCTTG
    TTACAAAATT ACCACTGTGC ACCCGATGAG CCTTTTATTC CTACGGATCT CATAGAAAAT
    GTTGTGGCTG TTGCTGAGAA CACAGCTGAT GAGCTCGCCC GCAGTGATGG CAGAGACGTG
    CAGCTGGAGG AAGACCCAGA CCTACAGCTG CCTTTTCTTC TGCCAGAAGA TGGCTATTCC
    TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG
    AGAGGATTTT GTAGATTGGT TCCTCACACA CGTTCACCAG GTACCTGGAC AATATATTTT
    GAAGGAGCAG ATTATGAAAG TCACCTTATG CGTGAGAACG CAGAACTGGC CCAGCCTCTG
    AGGAAGGAGC CTGAGATAAT CACTGTGACT CTGAAGAAGC AGAATGGAAT GGGCCTCAGC
    ATCGTTGCAG CCAAGGGTGC TGGCCAGGAT AAACTGGGAA TCTACGTTAA GTCGGTTGTC
    AAAGGAGGTG CTGCAGATGT GGATGGACGA TTAGCTGCTG GTGACCAACT CCTCAGTGTG
    GATGGACGAA GTCTGGTTGG ACTTTCTCAG GAAAGGGCAG CAGAACTCAT GACAAGAACC
    AGTTCCGTGG TTACACTGGA AGTTGCAAAG CAAGGTGCCA TCTATCACGG CCTGGCCACC
    CTCCTTAACC AGCCGTCCCC CATGATGCAA AGAATTTCAG ATCGTCGTGG CTCAGGTAAA
    CCCAGACCAA AGAGTGAAGG TTTTGAGCTC TATAATAATT CAGCTCAAAA TGGCTCCCCA
    GAGAGTCCTC AGATGCCTTG GACAGAGTAC AGTGAGCCAA AGAAACTGCC TGGGGATGAC
    AGGCTGATGA AAAACAGAGC GGACCACCGT TCCAGCCCCA ATGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAGGGTCC ATATACATCT GGAACAGCAG CTAAGATAAC ATCTGTCTCC
    ACTGGAAACC TCTGCACTGA GGAGCAGTCC CCTCCACCCA GACCTGAAGC CTATCCTATC
    CCTACTCAGA CATACACCAG AGAGTATTTT ACCTTTCCAG CTTCAAAGTC GCAGGATCGG
    ATGGCTCCTC CTCAGAGCCA GTGGCCAAAT TATGAGGAAA AACCACATGT GCACACAGAA
    AGTAATCATT CCAGTATTGC AATCCAGCGT GTTACCCGTT CCCAGGAAGA GCTTCGGGAA
    GAAAAGGTTT ACCAACTTGA GCGGCATCGA GTAGAGGCAG GCATGGATCG CAAGTGTGAC
    AGTGATATGT GGATCAATCA GAGCTCCTCA GTGGAATCCA GCACATCCAG CCAGGAACAC
    CTGAACCATT CTTCCAAGTC AGTTACCCCT GCTTCCACTC TGACCAAGAG TGGTCCTGGC
    CGTTGGAAAA CACCAGCAGC GGTGCTGCCC ACCCCTGTGG CTGTCTCTCA GCCCATTCGA
    ACAGACCTCC CTCCACCACC ACCTCCACCG CCGGTCCACT ACACCAGTGA GTTTGATGGT
    ATTCCAATGG ATTTGCCCCT CCCACCTCCT CCTGCCAACC AGGCAGGACC CCAGTCTGCT
    CAGGTGGCTG CTGCAGAGTG GAAAAAGAGA GAGGAGCACC AGAGGTGGTA TGAGAAAGAG
    AAGGCCCGCC TGGAGGAAGA ACGAGAGAGA AAGCGCAGGG AGCAGGAGCG GAAGTTGGGG
    CAGATGCGCT CTCAGACCCT GAACCCTGCT TCCTTCTCAC CTTTGGCCAC ACAGGCCAAG
    CCAGAAAAGC CTTCCACACT GCAGAGGCCC CAAGAAACAG TCATTCGGGA GCTTCAGCCC
    CAGCAGCAGC CCCGCACGAT CGAGCGCAAG GATCTGCAGT ATATAACCAT CAGTAAAGAG
    GAGCTGTCCT CTGGTGATAG TCTGTCTCCA GACCCCTGGA AACGAGATGC CAGGGAGAAG
    CTGGAGAAGC AACAGCAAAT GCACATTGTG GACATGCTAA GCAAGGAGAT CCACGAGCTG
    CAAAACAAGG TGGACCGTAC TGCGGAGGAG AGTGACCGCC TGCGGAAGCT TATGCTGGAG
    TGGCAGTTCC AGAAGAGACT ACAGGAGTCC AAACAGAAGG ATGAGGATGA TGACGAAGAG
    GAAGATGATG ATGTGGACAC CATGCTGATC ATGCAGCGTC TGGAGGCCGA GCGGAGAGCC
    AGGCAGACTG CTATGCCAGC AATCTCTGTT CTAGATTTGA TGCAGGATGA GGAGCGCAGG
    CGCCAGCAAC AGTTGGAAGA GATGCGGAAA CGAGAAGCAG AAGATCGAGT AAGACAAGAG
    GAAGATGGAC GCCATCAGGA GGAGGAGCGA GTAAAGAGAG ATGCTGAAGA AAAGAGGCGA
    CAGGAGGAAG GGTATTACAG CCGCCTAGAA GCTGAGAGGC GCCGACAGCA CGAAGAGGCA
    GCACGCAGGC TGCTGGAGCC TGAAGAGCCC GGGCTGAGCC GACCTCCACT TCCACGGGAC
    TATGAGCCCC CGTCCTTGTC CTCAGCACCC TGTGCCCCTC CTCCCCCACC TCAGCGAAAC
    GCATCCTACC TCAAAACACA GGTCCTCTCC CCAGACTCGC TGTTCACTGC CAAGTTTGTT
    GCGTATGATG AGGAGGAGGA GGACTACGGC CCAGCAGGTC AGGATAAGTA CCCCAGCACA
    CGGAAGTCTC ATGGGCACCT TCCTCTTGCC ACCCTTAAGC CGCAGCAGCC CCCCTGCCAG
    CCACGCCCAG CCTCGGATGG CATCTCCCTC TGCAACTCAT TCCAGCCACC CTTGGCCAAA
    GCCAACAGTA CAGCTTACAA AGCAGGCCAG CCCCCGCCCC TGCCAGGCAA ACCAAGCCTG
    TACCGTCCTG GCCACTGGAA AGGTCGAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172765.1
    CDS178..5904
    Translation

    Target ORF information:

    RefSeq Version XM_017317276.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317276.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGAAACAG
    AAGAACATTG CAGGAGCACT TGCTTTTTGG ATGGCAAATG CATCTGAGCT TCTCAACTTC
    ATCAAGCAGG ACCGAGACCT TAGTCGGATC ACGCTGGATG CCCAGGATGT CCTAGCACAC
    TTGGTTCAGA TGGCATTTAA GTACTTGGTT CATTGCCTTC AGTCAGAACT TAATAACTAC
    ATGCCAGCCT TTCTGGATGA TCCTGAGGAG AACAGTCTGC AAAGACCAAA AATAGATGAT
    GTGCTGCACA CACTCACAGG AGCCATGTCC TTGCTGCGGC GCTGCAGAGT CAATGCTGCC
    TTGACCATCC AGCTCTTCTC TCAGCTTTTC CACTTCATCA ATATGTGGCT GTTCAACAGA
    CTGGTGACTG ACCCAGATTC AGGCCTGTGC TCCCACTACT GGGGTGCCAT TATCCGCCAG
    CAGCTGGGGC ATATTGAAGC GTGGGCAGAG AAGCAGGGGC TAGAGCTGGC AGCTGACTGT
    CACTTGAGCA GAATTGTACA GGCTACAACA TTGCTTACGA TGGATAAGTA TGTACCTGAT
    GACATTCCAA ATATAAACAG CACATGCTTT AAGTTAAATT CTCTGCAGCT TCAAGCCTTG
    TTACAAAATT ACCACTGTGC ACCCGATGAG CCTTTTATTC CTACGGATCT CATAGAAAAT
    GTTGTGGCTG TTGCTGAGAA CACAGCTGAT GAGCTCGCCC GCAGTGATGG CAGAGACGTG
    CAGCTGGAGG AAGACCCAGA CCTACAGCTG CCTTTTCTTC TGCCAGAAGA TGGCTATTCC
    TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG
    AGAGGATTTT GTAGATTGGT TCCTCACACA CGTTCACCAG GTACCTGGAC AATATATTTT
    GAAGGAGCAG ATTATGAAAG TCACCTTATG CGTGAGAACG CAGAACTGGC CCAGCCTCTG
    AGGAAGGAGC CTGAGATAAT CACTGTGACT CTGAAGAAGC AGAATGGAAT GGGCCTCAGC
    ATCGTTGCAG CCAAGGGTGC TGGCCAGGAT AAACTGGGAA TCTACGTTAA GTCGGTTGTC
    AAAGGAGGTG CTGCAGATGT GGATGGACGA TTAGCTGCTG GTGACCAACT CCTCAGTGTG
    GATGGACGAA GTCTGGTTGG ACTTTCTCAG GAAAGGGCAG CAGAACTCAT GACAAGAACC
    AGTTCCGTGG TTACACTGGA AGTTGCAAAG CAAGGTGCCA TCTATCACGG CCTGGCCACC
    CTCCTTAACC AGCCGTCCCC CATGATGCAA AGAATTTCAG ATCGTCGTGG CTCAGGTAAA
    CCCAGACCAA AGAGTGAAGG TTTTGAGCTC TATAATAATT CAGCTCAAAA TGGCTCCCCA
    GAGAGTCCTC AGATGCCTTG GACAGAGTAC AGTGAGCCAA AGAAACTGCC TGGGGATGAC
    AGGCTGATGA AAAACAGAGC GGACCACCGT TCCAGCCCCA ATGTGGCAAA TCAGCCTCCT
    AGCCCTGGAG GGAAGGGTCC ATATACATCT GGAACAGCAG CTAAGATAAC ATCTGTCTCC
    ACTGGAAACC TCTGCACTGA GGAGCAGTCC CCTCCACCCA GACCTGAAGC CTATCCTATC
    CCTACTCAGA CATACACCAG AGAGTATTTT ACCTTTCCAG CTTCAAAGTC GCAGGATCGG
    ATGGCTCCTC CTCAGAGCCA GTGGCCAAAT TATGAGGAAA AACCACATGT GCACACAGAA
    AGTAATCATT CCAGTATTGC AATCCAGCGT GTTACCCGTT CCCAGGAAGA GCTTCGGGAA
    GAAAAGGTTT ACCAACTTGA GCGGCATCGA GTAGAGGCAG GCATGGATCG CAAGTGTGAC
    AGTGATATGT GGATCAATCA GAGCTCCTCA GTGGAATCCA GCACATCCAG CCAGGAACAC
    CTGAACCATT CTTCCAAGTC AGTTACCCCT GCTTCCACTC TGACCAAGAG TGGTCCTGGC
    CGTTGGAAAA CACCAGCAGC GGTGCTGCCC ACCCCTGTGG CTGTCTCTCA GCCCATTCGA
    ACAGACCTCC CTCCACCACC ACCTCCACCG CCGGTCCACT ACACCAGTGA GTTTGATGGT
    ATTCCAATGG ATTTGCCCCT CCCACCTCCT CCTGCCAACC AGGCAGGACC CCAGTCTGCT
    CAGGTGGCTG CTGCAGAGTG GAAAAAGAGA GAGGAGCACC AGAGGTGGTA TGAGAAAGAG
    AAGGCCCGCC TGGAGGAAGA ACGAGAGAGA AAGCGCAGGG AGCAGGAGCG GAAGTTGGGG
    CAGATGCGCT CTCAGACCCT GAACCCTGCT TCCTTCTCAC CTTTGGCCAC ACAGGCCAAG
    CCAGAAAAGC CTTCCACACT GCAGAGGCCC CAAGAAACAG TCATTCGGGA GCTTCAGCCC
    CAGCAGCAGC CCCGCACGAT CGAGCGCAAG GATCTGCAGT ATATAACCAT CAGTAAAGAG
    GAGCTGTCCT CTGGTGATAG TCTGTCTCCA GACCCCTGGA AACGAGATGC CAGGGAGAAG
    CTGGAGAAGC AACAGCAAAT GCACATTGTG GACATGCTAA GCAAGGAGAT CCACGAGCTG
    CAAAACAAGG TGGACCGTAC TGCGGAGGAG AGTGACCGCC TGCGGAAGCT TATGCTGGAG
    TGGCAGTTCC AGAAGAGACT ACAGGAGTCC AAACAGAAGG ATGAGGATGA TGACGAAGAG
    GAAGATGATG ATGTGGACAC CATGCTGATC ATGCAGCGTC TGGAGGCCGA GCGGAGAGCC
    AGGCAGACTG CTATGCCAGC AATCTCTGTT CTAGATTTGA TGCAGGATGA GGAGCGCAGG
    CGCCAGCAAC AGTTGGAAGA GATGCGGAAA CGAGAAGCAG AAGATCGAGT AAGACAAGAG
    GAAGATGGAC GCCATCAGGA GGAGGAGCGA GTAAAGAGAG ATGCTGAAGA AAAGAGGCGA
    CAGGAGGAAG GGTATTACAG CCGCCTAGAA GCTGAGAGGC GCCGACAGCA CGAAGAGGCA
    GCACGCAGGC TGCTGGAGCC TGAAGAGCCC GGGCTGAGCC GACCTCCACT TCCACGGGAC
    TATGAGCCCC CGTCCTTGTC CTCAGCACCC TGTGCCCCTC CTCCCCCACC TCAGCGAAAC
    GCATCCTACC TCAAAACACA GGTCCTCTCC CCAGACTCGC TGTTCACTGC CAAGTTTGTT
    GCGTATGATG AGGAGGAGGA GGACTACGGC CCAGCAGGTC AGGATAAGTA CCCCAGCACA
    CGGAAGTCTC ATGGGCACCT TCCTCTTGCC ACCCTTAAGC CGCAGCAGCC CCCCTGCCAG
    CCACGCCCAG CCTCGGATGG CATCTCCCTC TGCAACTCAT TCCAGCCACC CTTGGCCAAA
    GCCAACAGTA CAGCTTACAA AGCAGGCCAG CCCCCGCCCC TGCCAGGCAA ACCAAGCCTG
    TACCGTCCTG GCCACTGGAA AGGTCGAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu87108
    Clone ID Related Accession (Same CDS sequence) XM_030249526.1
    Accession Version XM_030249526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGAAACA GAAGAACATT GCAGGAGCAC TTGCTTTTTG GATGGCAAAT
    GCATCTGAGC TTCTCAACTT CATCAAGCAG GACCGAGACC TTAGTCGGAT CACGCTGGAT
    GCCCAGGATG TCCTAGCACA CTTGGTTCAG ATGGCATTTA AGTACTTGGT TCATTGCCTT
    CAGTCAGAAC TTAATAACTA CATGCCAGCC TTTCTGGATG ATCCTGAGGA GAACAGTCTG
    CAAAGACCAA AAATAGATGA TGTGCTGCAC ACACTCACAG GAGCCATGTC CTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CTTGACCATC CAGCTCTTCT CTCAGCTTTT CCACTTCATC
    AATATGTGGC TGTTCAACAG ACTGGTGACT GACCCAGATT CAGGCCTGTG CTCCCACTAC
    TGGGGTGCCA TTATCCGCCA GCAGCTGGGG CATATTGAAG CGTGGGCAGA GAAGCAGGGG
    CTAGAGCTGG CAGCTGACTG TCACTTGAGC AGAATTGTAC AGGCTACAAC ATTGCTTACG
    ATGGATAAGT ATGTACCTGA TGACATTCCA AATATAAACA GCACATGCTT TAAGTTAAAT
    TCTCTGCAGC TTCAAGCCTT GTTACAAAAT TACCACTGTG CACCCGATGA GCCTTTTATT
    CCTACGGATC TCATAGAAAA TGTTGTGGCT GTTGCTGAGA ACACAGCTGA TGAGCTCGCC
    CGCAGTGATG GCAGAGACGT GCAGCTGGAG GAAGACCCAG ACCTACAGCT GCCTTTTCTT
    CTGCCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGTAGATTGG TTCCTCACAC ACGTTCACCA
    GGTACCTGGA CAATATATTT TGAAGGAGCA GATTATGAAA GTCACCTTAT GCGTGAGAAC
    GCAGAACTGG CCCAGCCTCT GAGGAAGGAG CCTGAGATAA TCACTGTGAC TCTGAAGAAG
    CAGAATGGAA TGGGCCTCAG CATCGTTGCA GCCAAGGGTG CTGGCCAGGA TAAACTGGGA
    ATCTACGTTA AGTCGGTTGT CAAAGGAGGT GCTGCAGATG TGGATGGACG ATTAGCTGCT
    GGTGACCAAC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTTTCTCA GGAAAGGGCA
    GCAGAACTCA TGACAAGAAC CAGTTCCGTG GTTACACTGG AAGTTGCAAA GCAAGGTGCC
    ATCTATCACG GCCTGGCCAC CCTCCTTAAC CAGCCGTCCC CCATGATGCA AAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCAGACCA AAGAGTGAAG GTTTTGAGCT CTATAATAAT
    TCAGCTCAAA ATGGCTCCCC AGAGAGTCCT CAGATGCCTT GGACAGAGTA CAGTGAGCCA
    AAGAAACTGC CTGGGGATGA CAGGCTGATG AAAAACAGAG CGGACCACCG TTCCAGCCCC
    AATGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGGGTC CATATACATC TGGAACAGCA
    GCTAAGATAA CATCTGTCTC CACTGGAAAC CTCTGCACTG AGGAGCAGTC CCCTCCACCC
    AGACCTGAAG CCTATCCTAT CCCTACTCAG ACATACACCA GAGAGTATTT TACCTTTCCA
    GCTTCAAAGT CGCAGGATCG GATGGCTCCT CCTCAGAGCC AGTGGCCAAA TTATGAGGAA
    AAACCACATG TGCACACAGA AAGTAATCAT TCCAGTATTG CAATCCAGCG TGTTACCCGT
    TCCCAGGAAG AGCTTCGGGA AGAAAAGGTT TACCAACTTG AGCGGCATCG AGTAGAGGCA
    GGCATGGATC GCAAGTGTGA CAGTGATATG TGGATCAATC AGAGCTCCTC AGTGGAATCC
    AGCACATCCA GCCAGGAACA CCTGAACCAT TCTTCCAAGT CAGTTACCCC TGCTTCCACT
    CTGACCAAGA GTGGTCCTGG CCGTTGGAAA ACACCAGCAG CGGTGCTGCC CACCCCTGTG
    GCTGTCTCTC AGCCCATTCG AACAGACCTC CCTCCACCAC CACCTCCACC GCCGGTCCAC
    TACACCAGTG AGTTTGATGG TATTCCAATG GATTTGCCCC TCCCACCTCC TCCTGCCAAC
    CAGGCAGGAC CCCAGTCTGC TCAGGTGGCT GCTGCAGAGT GGAAAAAGAG AGAGGAGCAC
    CAGAGGTGGT ATGAGAAAGA GAAGGCCCGC CTGGAGGAAG AACGAGAGAG AAAGCGCAGG
    GAGCAGGAGC GGAAGTTGGG GCAGATGCGC TCTCAGACCC TGAACCCTGC TTCCTTCTCA
    CCTTTGGCCA CACAGGCCAA GCCAGAAAAG CCTTCCACAC TGCAGAGGCC CCAAGAAACA
    GTCATTCGGG AGCTTCAGCC CCAGCAGCAG CCCCGCACGA TCGAGCGCAA GGATCTGCAG
    TATATAACCA TCAGTAAAGA GGAGCTGTCC TCTGGTGATA GTCTGTCTCC AGACCCCTGG
    AAACGAGATG CCAGGGAGAA GCTGGAGAAG CAACAGCAAA TGCACATTGT GGACATGCTA
    AGCAAGGAGA TCCACGAGCT GCAAAACAAG GTGGACCGTA CTGCGGAGGA GAGTGACCGC
    CTGCGGAAGC TTATGCTGGA GTGGCAGTTC CAGAAGAGAC TACAGGAGTC CAAACAGAAG
    GATGAGGATG ATGACGAAGA GGAAGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGT
    CTGGAGGCCG AGCGGAGAGC CAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG
    ATGCAGGATG AGGAGCGCAG GCGCCAGCAA CAGTTGGAAG AGATGCGGAA ACGAGAAGCA
    GAAGATCGAG TAAGACAAGA GGAAGATGGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA
    GATGCTGAAG AAAAGAGGCG ACAGGAGGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG
    CGCCGACAGC ACGAAGAGGC AGCACGCAGG CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC
    CGACCTCCAC TTCCACGGGA CTATGAGCCC CCGTCCTTGT CCTCAGCACC CTGTGCCCCT
    CCTCCCCCAC CTCAGCGAAA CGCATCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG
    CTGTTCACTG CCAAGTTTGT TGCGTATGAT GAGGAGGAGG AGGACTACGG CCCAGCAGGT
    CAGGATAAGT ACCCCAGCAC ACGGAAGTCT CATGGGCACC TTCCTCTTGC CACCCTTAAG
    CCGCAGCAGC CCCCCTGCCA GCCACGCCCA GCCTCGGATG GCATCTCCCT CTGCAACTCA
    TTCCAGCCAC CCTTGGCCAA AGCCAACAGT ACAGCTTACA AAGCAGGCCA GCCCCCGCCC
    CTGCCAGGCA AACCAAGCCT GTACCGTCCT GGCCACTGGA AAGGTCGAGG ACCAAACTCT
    TACTCGGGAT CTGCAGGCAC AGCTGTGGGA GCCTATGATG CCCCTCGGGA AGCGAGAGAG
    AAACTGACTA GGAGCCAAGA CGCAGACTTA CCTGGCAGTT CTGGAGCCCC TGAAAATCTG
    ACATTTAAAG AGCGTCAGCG CCTTTTTTCA CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA
    GCTTCTCGTA AATTAACAGA GCTCGAGAAT GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105386.1
    CDS284..5971
    Translation

    Target ORF information:

    RefSeq Version XM_030249526.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGAAACA GAAGAACATT GCAGGAGCAC TTGCTTTTTG GATGGCAAAT
    GCATCTGAGC TTCTCAACTT CATCAAGCAG GACCGAGACC TTAGTCGGAT CACGCTGGAT
    GCCCAGGATG TCCTAGCACA CTTGGTTCAG ATGGCATTTA AGTACTTGGT TCATTGCCTT
    CAGTCAGAAC TTAATAACTA CATGCCAGCC TTTCTGGATG ATCCTGAGGA GAACAGTCTG
    CAAAGACCAA AAATAGATGA TGTGCTGCAC ACACTCACAG GAGCCATGTC CTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CTTGACCATC CAGCTCTTCT CTCAGCTTTT CCACTTCATC
    AATATGTGGC TGTTCAACAG ACTGGTGACT GACCCAGATT CAGGCCTGTG CTCCCACTAC
    TGGGGTGCCA TTATCCGCCA GCAGCTGGGG CATATTGAAG CGTGGGCAGA GAAGCAGGGG
    CTAGAGCTGG CAGCTGACTG TCACTTGAGC AGAATTGTAC AGGCTACAAC ATTGCTTACG
    ATGGATAAGT ATGTACCTGA TGACATTCCA AATATAAACA GCACATGCTT TAAGTTAAAT
    TCTCTGCAGC TTCAAGCCTT GTTACAAAAT TACCACTGTG CACCCGATGA GCCTTTTATT
    CCTACGGATC TCATAGAAAA TGTTGTGGCT GTTGCTGAGA ACACAGCTGA TGAGCTCGCC
    CGCAGTGATG GCAGAGACGT GCAGCTGGAG GAAGACCCAG ACCTACAGCT GCCTTTTCTT
    CTGCCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGTAGATTGG TTCCTCACAC ACGTTCACCA
    GGTACCTGGA CAATATATTT TGAAGGAGCA GATTATGAAA GTCACCTTAT GCGTGAGAAC
    GCAGAACTGG CCCAGCCTCT GAGGAAGGAG CCTGAGATAA TCACTGTGAC TCTGAAGAAG
    CAGAATGGAA TGGGCCTCAG CATCGTTGCA GCCAAGGGTG CTGGCCAGGA TAAACTGGGA
    ATCTACGTTA AGTCGGTTGT CAAAGGAGGT GCTGCAGATG TGGATGGACG ATTAGCTGCT
    GGTGACCAAC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTTTCTCA GGAAAGGGCA
    GCAGAACTCA TGACAAGAAC CAGTTCCGTG GTTACACTGG AAGTTGCAAA GCAAGGTGCC
    ATCTATCACG GCCTGGCCAC CCTCCTTAAC CAGCCGTCCC CCATGATGCA AAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCAGACCA AAGAGTGAAG GTTTTGAGCT CTATAATAAT
    TCAGCTCAAA ATGGCTCCCC AGAGAGTCCT CAGATGCCTT GGACAGAGTA CAGTGAGCCA
    AAGAAACTGC CTGGGGATGA CAGGCTGATG AAAAACAGAG CGGACCACCG TTCCAGCCCC
    AATGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGGGTC CATATACATC TGGAACAGCA
    GCTAAGATAA CATCTGTCTC CACTGGAAAC CTCTGCACTG AGGAGCAGTC CCCTCCACCC
    AGACCTGAAG CCTATCCTAT CCCTACTCAG ACATACACCA GAGAGTATTT TACCTTTCCA
    GCTTCAAAGT CGCAGGATCG GATGGCTCCT CCTCAGAGCC AGTGGCCAAA TTATGAGGAA
    AAACCACATG TGCACACAGA AAGTAATCAT TCCAGTATTG CAATCCAGCG TGTTACCCGT
    TCCCAGGAAG AGCTTCGGGA AGAAAAGGTT TACCAACTTG AGCGGCATCG AGTAGAGGCA
    GGCATGGATC GCAAGTGTGA CAGTGATATG TGGATCAATC AGAGCTCCTC AGTGGAATCC
    AGCACATCCA GCCAGGAACA CCTGAACCAT TCTTCCAAGT CAGTTACCCC TGCTTCCACT
    CTGACCAAGA GTGGTCCTGG CCGTTGGAAA ACACCAGCAG CGGTGCTGCC CACCCCTGTG
    GCTGTCTCTC AGCCCATTCG AACAGACCTC CCTCCACCAC CACCTCCACC GCCGGTCCAC
    TACACCAGTG AGTTTGATGG TATTCCAATG GATTTGCCCC TCCCACCTCC TCCTGCCAAC
    CAGGCAGGAC CCCAGTCTGC TCAGGTGGCT GCTGCAGAGT GGAAAAAGAG AGAGGAGCAC
    CAGAGGTGGT ATGAGAAAGA GAAGGCCCGC CTGGAGGAAG AACGAGAGAG AAAGCGCAGG
    GAGCAGGAGC GGAAGTTGGG GCAGATGCGC TCTCAGACCC TGAACCCTGC TTCCTTCTCA
    CCTTTGGCCA CACAGGCCAA GCCAGAAAAG CCTTCCACAC TGCAGAGGCC CCAAGAAACA
    GTCATTCGGG AGCTTCAGCC CCAGCAGCAG CCCCGCACGA TCGAGCGCAA GGATCTGCAG
    TATATAACCA TCAGTAAAGA GGAGCTGTCC TCTGGTGATA GTCTGTCTCC AGACCCCTGG
    AAACGAGATG CCAGGGAGAA GCTGGAGAAG CAACAGCAAA TGCACATTGT GGACATGCTA
    AGCAAGGAGA TCCACGAGCT GCAAAACAAG GTGGACCGTA CTGCGGAGGA GAGTGACCGC
    CTGCGGAAGC TTATGCTGGA GTGGCAGTTC CAGAAGAGAC TACAGGAGTC CAAACAGAAG
    GATGAGGATG ATGACGAAGA GGAAGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGT
    CTGGAGGCCG AGCGGAGAGC CAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG
    ATGCAGGATG AGGAGCGCAG GCGCCAGCAA CAGTTGGAAG AGATGCGGAA ACGAGAAGCA
    GAAGATCGAG TAAGACAAGA GGAAGATGGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA
    GATGCTGAAG AAAAGAGGCG ACAGGAGGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG
    CGCCGACAGC ACGAAGAGGC AGCACGCAGG CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC
    CGACCTCCAC TTCCACGGGA CTATGAGCCC CCGTCCTTGT CCTCAGCACC CTGTGCCCCT
    CCTCCCCCAC CTCAGCGAAA CGCATCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG
    CTGTTCACTG CCAAGTTTGT TGCGTATGAT GAGGAGGAGG AGGACTACGG CCCAGCAGGT
    CAGGATAAGT ACCCCAGCAC ACGGAAGTCT CATGGGCACC TTCCTCTTGC CACCCTTAAG
    CCGCAGCAGC CCCCCTGCCA GCCACGCCCA GCCTCGGATG GCATCTCCCT CTGCAACTCA
    TTCCAGCCAC CCTTGGCCAA AGCCAACAGT ACAGCTTACA AAGCAGGCCA GCCCCCGCCC
    CTGCCAGGCA AACCAAGCCT GTACCGTCCT GGCCACTGGA AAGGTCGAGG ACCAAACTCT
    TACTCGGGAT CTGCAGGCAC AGCTGTGGGA GCCTATGATG CCCCTCGGGA AGCGAGAGAG
    AAACTGACTA GGAGCCAAGA CGCAGACTTA CCTGGCAGTT CTGGAGCCCC TGAAAATCTG
    ACATTTAAAG AGCGTCAGCG CCTTTTTTCA CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA
    GCTTCTCGTA AATTAACAGA GCTCGAGAAT GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu87109
    Clone ID Related Accession (Same CDS sequence) XM_030249527.1
    Accession Version XM_030249527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGACCAAACT CTTACTCGGG ATCTGCAGGC ACAGCTGTGG GAGCCTATGA TGCCCCTCGG
    GAAGCGAGAG AGAAACTGAC TAGGAGCCAA GACGCAGACT TACCTGGCAG TTCTGGAGCC
    CCTGAAAATC TGACATTTAA AGAGCGTCAG CGCCTTTTTT CACAAGGGCA GGATGTGTCT
    GACAAAGTGA AAGCTTCTCG TAAATTAACA GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105387.1
    CDS284..5803
    Translation

    Target ORF information:

    RefSeq Version XM_030249527.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGACCAAACT CTTACTCGGG ATCTGCAGGC ACAGCTGTGG GAGCCTATGA TGCCCCTCGG
    GAAGCGAGAG AGAAACTGAC TAGGAGCCAA GACGCAGACT TACCTGGCAG TTCTGGAGCC
    CCTGAAAATC TGACATTTAA AGAGCGTCAG CGCCTTTTTT CACAAGGGCA GGATGTGTCT
    GACAAAGTGA AAGCTTCTCG TAAATTAACA GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu87110
    Clone ID Related Accession (Same CDS sequence) XM_030249528.1
    Accession Version XM_030249528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5442bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product afadin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGAGCA
    CTTGCTTTTT GGATGGCAAA TGCATCTGAG CTTCTCAACT TCATCAAGCA GGACCGAGAC
    CTTAGTCGGA TCACGCTGGA TGCCCAGGAT GTCCTAGCAC ACTTGGTTCA GATGGCATTT
    AAGTACTTGG TTCATTGCCT TCAGTCAGAA CTTAATAACT ACATGCCAGC CTTTCTGGAT
    GATCCTGAGG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACACTCACA
    GGAGCCATGT CCTTGCTGCG GCGCTGCAGA GTCAATGCTG CCTTGACCAT CCAGCTCTTC
    TCTCAGCTTT TCCACTTCAT CAATATGTGG CTGTTCAACA GACTGGTGAC TGACCCAGAT
    TCAGGCCTGT GCTCCCACTA CTGGGGTGCC ATTATCCGCC AGCAGCTGGG GCATATTGAA
    GCGTGGGCAG AGAAGCAGGG GCTAGAGCTG GCAGCTGACT GTCACTTGAG CAGAATTGTA
    CAGGCTACAA CATTGCTTAC GATGGATAAG TATGTACCTG ATGACATTCC AAATATAAAC
    AGCACATGCT TTAAGTTAAA TTCTCTGCAG CTTCAAGCCT TGTTACAAAA TTACCACTGT
    GCACCCGATG AGCCTTTTAT TCCTACGGAT CTCATAGAAA ATGTTGTGGC TGTTGCTGAG
    AACACAGCTG ATGAGCTCGC CCGCAGTGAT GGCAGAGACG TGCAGCTGGA GGAAGACCCA
    GACCTACAGC TGCCTTTTCT TCTGCCAGAA GATGGCTATT CCTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGTAGATTG
    GTTCCTCACA CACGTTCACC AGGTACCTGG ACAATATATT TTGAAGGAGC AGATTATGAA
    AGTCACCTTA TGCGTGAGAA CGCAGAACTG GCCCAGCCTC TGAGGAAGGA GCCTGAGATA
    ATCACTGTGA CTCTGAAGAA GCAGAATGGA ATGGGCCTCA GCATCGTTGC AGCCAAGGGT
    GCTGGCCAGG ATAAACTGGG AATCTACGTT AAGTCGGTTG TCAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GATTAGCTGC TGGTGACCAA CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTTTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CCAGTTCCGT GGTTACACTG
    GAAGTTGCAA AGCAAGGTGC CATCTATCAC GGCCTGGCCA CCCTCCTTAA CCAGCCGTCC
    CCCATGATGC AAAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCAGACC AAAGAGTGAA
    GGTTTTGAGC TCTATAATAA TTCAGCTCAA AATGGCTCCC CAGAGAGTCC TCAGATGCCT
    TGGACAGAGT ACAGTGAGCC AAAGAAACTG CCTGGGGATG ACAGGCTGAT GAAAAACAGA
    GCGGACCACC GTTCCAGCCC CAATGTGGCA AATCAGCCTC CTAGCCCTGG AGGGAAGGGT
    CCATATACAT CTGGAACAGC AGCTAAGATA ACATCTGTCT CCACTGGAAA CCTCTGCACT
    GAGGAGCAGT CCCCTCCACC CAGACCTGAA GCCTATCCTA TCCCTACTCA GACATACACC
    AGAGAGTATT TTACCTTTCC AGCTTCAAAG TCGCAGGATC GGATGGCTCC TCCTCAGAGC
    CAGTGGCCAA ATTATGAGGA AAAACCACAT GTGCACACAG AAAGTAATCA TTCCAGTATT
    GCAATCCAGC GTGTTACCCG TTCCCAGGAA GAGCTTCGGG AAGAAAAGGT TTACCAACTT
    GAGCGGCATC GAGTAGAGGC AGGCATGGAT CGCAAGTGTG ACAGTGATAT GTGGATCAAT
    CAGAGCTCCT CAGTGGAATC CAGCACATCC AGCCAGGAAC ACCTGAACCA TTCTTCCAAG
    TCAGTTACCC CTGCTTCCAC TCTGACCAAG AGTGGTCCTG GCCGTTGGAA AACACCAGCA
    GCGGTGCTGC CCACCCCTGT GGCTGTCTCT CAGCCCATTC GAACAGACCT CCCTCCACCA
    CCACCTCCAC CGCCGGTCCA CTACACCAGT GAGTTTGATG GTATTCCAAT GGATTTGCCC
    CTCCCACCTC CTCCTGCCAA CCAGGCAGGA CCCCAGTCTG CTCAGGTGGC TGCTGCAGAG
    TGGAAAAAGA GAGAGGAGCA CCAGAGGTGG TATGAGAAAG AGAAGGCCCG CCTGGAGGAA
    GAACGAGAGA GAAAGCGCAG GGAGCAGGAG CGGAAGTTGG GGCAGATGCG CTCTCAGACC
    CTGAACCCTG CTTCCTTCTC ACCTTTGGCC ACACAGGCCA AGCCAGAAAA GCCTTCCACA
    CTGCAGAGGC CCCAAGAAAC AGTCATTCGG GAGCTTCAGC CCCAGCAGCA GCCCCGCACG
    ATCGAGCGCA AGGATCTGCA GTATATAACC ATCAGTAAAG AGGAGCTGTC CTCTGGTGAT
    AGTCTGTCTC CAGACCCCTG GAAACGAGAT GCCAGGGAGA AGCTGGAGAA GCAACAGCAA
    ATGCACATTG TGGACATGCT AAGCAAGGAG ATCCACGAGC TGCAAAACAA GGTGGACCGT
    ACTGCGGAGG AGAGTGACCG CCTGCGGAAG CTTATGCTGG AGTGGCAGTT CCAGAAGAGA
    CTACAGGAGT CCAAACAGAA GGATGAGGAT GATGACGAAG AGGAAGATGA TGATGTGGAC
    ACCATGCTGA TCATGCAGCG TCTGGAGGCC GAGCGGAGAG CCAGGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030105388.1
    CDS284..5725
    Translation

    Target ORF information:

    RefSeq Version XM_030249528.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGAGCA
    CTTGCTTTTT GGATGGCAAA TGCATCTGAG CTTCTCAACT TCATCAAGCA GGACCGAGAC
    CTTAGTCGGA TCACGCTGGA TGCCCAGGAT GTCCTAGCAC ACTTGGTTCA GATGGCATTT
    AAGTACTTGG TTCATTGCCT TCAGTCAGAA CTTAATAACT ACATGCCAGC CTTTCTGGAT
    GATCCTGAGG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACACTCACA
    GGAGCCATGT CCTTGCTGCG GCGCTGCAGA GTCAATGCTG CCTTGACCAT CCAGCTCTTC
    TCTCAGCTTT TCCACTTCAT CAATATGTGG CTGTTCAACA GACTGGTGAC TGACCCAGAT
    TCAGGCCTGT GCTCCCACTA CTGGGGTGCC ATTATCCGCC AGCAGCTGGG GCATATTGAA
    GCGTGGGCAG AGAAGCAGGG GCTAGAGCTG GCAGCTGACT GTCACTTGAG CAGAATTGTA
    CAGGCTACAA CATTGCTTAC GATGGATAAG TATGTACCTG ATGACATTCC AAATATAAAC
    AGCACATGCT TTAAGTTAAA TTCTCTGCAG CTTCAAGCCT TGTTACAAAA TTACCACTGT
    GCACCCGATG AGCCTTTTAT TCCTACGGAT CTCATAGAAA ATGTTGTGGC TGTTGCTGAG
    AACACAGCTG ATGAGCTCGC CCGCAGTGAT GGCAGAGACG TGCAGCTGGA GGAAGACCCA
    GACCTACAGC TGCCTTTTCT TCTGCCAGAA GATGGCTATT CCTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGTAGATTG
    GTTCCTCACA CACGTTCACC AGGTACCTGG ACAATATATT TTGAAGGAGC AGATTATGAA
    AGTCACCTTA TGCGTGAGAA CGCAGAACTG GCCCAGCCTC TGAGGAAGGA GCCTGAGATA
    ATCACTGTGA CTCTGAAGAA GCAGAATGGA ATGGGCCTCA GCATCGTTGC AGCCAAGGGT
    GCTGGCCAGG ATAAACTGGG AATCTACGTT AAGTCGGTTG TCAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GATTAGCTGC TGGTGACCAA CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTTTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CCAGTTCCGT GGTTACACTG
    GAAGTTGCAA AGCAAGGTGC CATCTATCAC GGCCTGGCCA CCCTCCTTAA CCAGCCGTCC
    CCCATGATGC AAAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCAGACC AAAGAGTGAA
    GGTTTTGAGC TCTATAATAA TTCAGCTCAA AATGGCTCCC CAGAGAGTCC TCAGATGCCT
    TGGACAGAGT ACAGTGAGCC AAAGAAACTG CCTGGGGATG ACAGGCTGAT GAAAAACAGA
    GCGGACCACC GTTCCAGCCC CAATGTGGCA AATCAGCCTC CTAGCCCTGG AGGGAAGGGT
    CCATATACAT CTGGAACAGC AGCTAAGATA ACATCTGTCT CCACTGGAAA CCTCTGCACT
    GAGGAGCAGT CCCCTCCACC CAGACCTGAA GCCTATCCTA TCCCTACTCA GACATACACC
    AGAGAGTATT TTACCTTTCC AGCTTCAAAG TCGCAGGATC GGATGGCTCC TCCTCAGAGC
    CAGTGGCCAA ATTATGAGGA AAAACCACAT GTGCACACAG AAAGTAATCA TTCCAGTATT
    GCAATCCAGC GTGTTACCCG TTCCCAGGAA GAGCTTCGGG AAGAAAAGGT TTACCAACTT
    GAGCGGCATC GAGTAGAGGC AGGCATGGAT CGCAAGTGTG ACAGTGATAT GTGGATCAAT
    CAGAGCTCCT CAGTGGAATC CAGCACATCC AGCCAGGAAC ACCTGAACCA TTCTTCCAAG
    TCAGTTACCC CTGCTTCCAC TCTGACCAAG AGTGGTCCTG GCCGTTGGAA AACACCAGCA
    GCGGTGCTGC CCACCCCTGT GGCTGTCTCT CAGCCCATTC GAACAGACCT CCCTCCACCA
    CCACCTCCAC CGCCGGTCCA CTACACCAGT GAGTTTGATG GTATTCCAAT GGATTTGCCC
    CTCCCACCTC CTCCTGCCAA CCAGGCAGGA CCCCAGTCTG CTCAGGTGGC TGCTGCAGAG
    TGGAAAAAGA GAGAGGAGCA CCAGAGGTGG TATGAGAAAG AGAAGGCCCG CCTGGAGGAA
    GAACGAGAGA GAAAGCGCAG GGAGCAGGAG CGGAAGTTGG GGCAGATGCG CTCTCAGACC
    CTGAACCCTG CTTCCTTCTC ACCTTTGGCC ACACAGGCCA AGCCAGAAAA GCCTTCCACA
    CTGCAGAGGC CCCAAGAAAC AGTCATTCGG GAGCTTCAGC CCCAGCAGCA GCCCCGCACG
    ATCGAGCGCA AGGATCTGCA GTATATAACC ATCAGTAAAG AGGAGCTGTC CTCTGGTGAT
    AGTCTGTCTC CAGACCCCTG GAAACGAGAT GCCAGGGAGA AGCTGGAGAA GCAACAGCAA
    ATGCACATTG TGGACATGCT AAGCAAGGAG ATCCACGAGC TGCAAAACAA GGTGGACCGT
    ACTGCGGAGG AGAGTGACCG CCTGCGGAAG CTTATGCTGG AGTGGCAGTT CCAGAAGAGA
    CTACAGGAGT CCAAACAGAA GGATGAGGAT GATGACGAAG AGGAAGATGA TGATGTGGAC
    ACCATGCTGA TCATGCAGCG TCTGGAGGCC GAGCGGAGAG CCAGGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22404
    Clone ID Related Accession (Same CDS sequence) NM_010806.1
    Accession Version NM_010806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product afadin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ165098.1, AF172447.1, CF538789.1, CK637291.1, CN537179.1, CN538841.1, BU611478.1, CO433521.1, CN529812.1, BI991173.1, BQ929757.1, CB181965.1 and CO042367.1. On or before Jul 25, 2007 this sequence version replaced XM_911010.3, XM_890447.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849390 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGACCAA ACTCTTACTC GGGATCTGCA GGCACAGCTG TGGGAGCCTA TGATGCCCCT
    CGGGAAGCGA GAGAGAAACT GACTAGGAGC CAAGACGCAG ACTTACCTGG CAGTTCTGGA
    GCCCCTGAAA ATCTGACATT TAAAGAGCGT CAGCGCCTTT TTTCACAAGG GCAGGATGTG
    TCTGACAAAG TGAAAGCTTC TCGTAAATTA ACAGAGCTCG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_034936.1
    CDS134..5596
    Translation

    Target ORF information:

    RefSeq Version NM_010806.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus afadin, adherens junction formation factor (Afdn), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_010806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGACCAA ACTCTTACTC GGGATCTGCA GGCACAGCTG TGGGAGCCTA TGATGCCCCT
    CGGGAAGCGA GAGAGAAACT GACTAGGAGC CAAGACGCAG ACTTACCTGG CAGTTCTGGA
    GCCCCTGAAA ATCTGACATT TAAAGAGCGT CAGCGCCTTT TTTCACAAGG GCAGGATGTG
    TCTGACAAAG TGAAAGCTTC TCGTAAATTA ACAGAGCTCG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53206
    Clone ID Related Accession (Same CDS sequence) XM_006523752.2 , XM_006523752.3
    Accession Version XM_006523752.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGTCAGG ATAAGTACCC CAGCACACGG AAGTCTCATG GGCACCTTCC TCTTGCCACC
    CTTAAGCCGC AGCAGCCCCC CTGCCAGCCA CGCCCAGCCT CGGATGGCAT CTCCCTCTGC
    AACTCATTCC AGCCACCCTT GGCCAAAGCC AACAGTACAG CTTACAAAGC AGGCCAGCCC
    CCGCCCCTGC CAGGCAAACC AAGCCTGTAC CGTCCTGGCC ACTGGAAAGG TCGAGGACCA
    AACTCTTACT CGGGATCTGC AGGCACAGCT GTGGGAGCCT ATGATGCCCC TCGGGAAGCG
    AGAGAGAAAC TGACTAGGAG CCAAGACGCA GACTTACCTG GCAGTTCTGG AGCCCCTGAA
    AATCTGACAT TTAAAGAGCG TCAGCGCCTT TTTTCACAAG GGCAGGATGT GTCTGACAAA
    GTGAAAGCTT CTCGTAAATT AACAGAGCTC GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523815.1
    CDS131..5884
    Translation

    Target ORF information:

    RefSeq Version XM_006523752.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGTCAGG ATAAGTACCC CAGCACACGG AAGTCTCATG GGCACCTTCC TCTTGCCACC
    CTTAAGCCGC AGCAGCCCCC CTGCCAGCCA CGCCCAGCCT CGGATGGCAT CTCCCTCTGC
    AACTCATTCC AGCCACCCTT GGCCAAAGCC AACAGTACAG CTTACAAAGC AGGCCAGCCC
    CCGCCCCTGC CAGGCAAACC AAGCCTGTAC CGTCCTGGCC ACTGGAAAGG TCGAGGACCA
    AACTCTTACT CGGGATCTGC AGGCACAGCT GTGGGAGCCT ATGATGCCCC TCGGGAAGCG
    AGAGAGAAAC TGACTAGGAG CCAAGACGCA GACTTACCTG GCAGTTCTGG AGCCCCTGAA
    AATCTGACAT TTAAAGAGCG TCAGCGCCTT TTTTCACAAG GGCAGGATGT GTCTGACAAA
    GTGAAAGCTT CTCGTAAATT AACAGAGCTC GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53207
    Clone ID Related Accession (Same CDS sequence) XM_006523753.2 , XM_006523753.3
    Accession Version XM_006523753.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523816.1
    CDS131..5881
    Translation

    Target ORF information:

    RefSeq Version XM_006523753.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523753.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53208
    Clone ID Related Accession (Same CDS sequence) XM_006523754.3 , XM_006523754.2
    Accession Version XM_006523754.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523817.1
    CDS131..5881
    Translation

    Target ORF information:

    RefSeq Version XM_006523754.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523754.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGCAG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53209
    Clone ID Related Accession (Same CDS sequence) XM_006523755.3 , XM_006523755.2
    Accession Version XM_006523755.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523818.1
    CDS131..5881
    Translation

    Target ORF information:

    RefSeq Version XM_006523755.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523755.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ACTGCTATGC CAGCAATCTC TGTTCTAGAT
    TTGATGCAGG ATGAGGAGCG CAGGCGCCAG CAACAGTTGG AAGAGATGCG GAAACGAGAA
    GCAGAAGATC GAGTAAGACA AGAGGAAGAT GGACGCCATC AGGAGGAGGA GCGAGTAAAG
    AGAGATGCTG AAGAAAAGAG GCGACAGGAG GAAGGGTATT ACAGCCGCCT AGAAGCTGAG
    AGGCGCCGAC AGCACGAAGA GGCAGCACGC AGGCTGCTGG AGCCTGAAGA GCCCGGGCTG
    AGCCGACCTC CACTTCCACG GGACTATGAG CCCCCGTCCT TGTCCTCAGC ACCCTGTGCC
    CCTCCTCCCC CACCTCAGCG AAACGCATCC TACCTCAAAA CACAGGTCCT CTCCCCAGAC
    TCGCTGTTCA CTGCCAAGTT TGTTGCGTAT GATGAGGAGG AGGAGGACTA CGGCCCAGCA
    GGTCAGGATA AGTACCCCAG CACACGGAAG TCTCATGGGC ACCTTCCTCT TGCCACCCTT
    AAGCCGCAGC AGCCCCCCTG CCAGCCACGC CCAGCCTCGG ATGGCATCTC CCTCTGCAAC
    TCATTCCAGC CACCCTTGGC CAAAGCCAAC AGTACAGCTT ACAAAGCAGG CCAGCCCCCG
    CCCCTGCCAG GCAAACCAAG CCTGTACCGT CCTGGCCACT GGAAAGGTCG AGGACCAAAC
    TCTTACTCGG GATCTGCAGG CACAGCTGTG GGAGCCTATG ATGCCCCTCG GGAAGCGAGA
    GAGAAACTGA CTAGGAGCCA AGACGCAGAC TTACCTGGCA GTTCTGGAGC CCCTGAAAAT
    CTGACATTTA AAGAGCGTCA GCGCCTTTTT TCACAAGGGC AGGATGTGTC TGACAAAGTG
    AAAGCTTCTC GTAAATTAAC AGAGCTCGAG AATGAACTGA ACACAAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53210
    Clone ID Related Accession (Same CDS sequence) XM_006523756.3 , XM_006523756.2
    Accession Version XM_006523756.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAACATT GCAGGAGCAC TTGCTTTTTG GATGGCAAAT
    GCATCTGAGC TTCTCAACTT CATCAAGCAG GACCGAGACC TTAGTCGGAT CACGCTGGAT
    GCCCAGGATG TCCTAGCACA CTTGGTTCAG ATGGCATTTA AGTACTTGGT TCATTGCCTT
    CAGTCAGAAC TTAATAACTA CATGCCAGCC TTTCTGGATG ATCCTGAGGA GAACAGTCTG
    CAAAGACCAA AAATAGATGA TGTGCTGCAC ACACTCACAG GAGCCATGTC CTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CTTGACCATC CAGCTCTTCT CTCAGCTTTT CCACTTCATC
    AATATGTGGC TGTTCAACAG ACTGGTGACT GACCCAGATT CAGGCCTGTG CTCCCACTAC
    TGGGGTGCCA TTATCCGCCA GCAGCTGGGG CATATTGAAG CGTGGGCAGA GAAGCAGGGG
    CTAGAGCTGG CAGCTGACTG TCACTTGAGC AGAATTGTAC AGGCTACAAC ATTGCTTACG
    ATGGATAAGT ATGTACCTGA TGACATTCCA AATATAAACA GCACATGCTT TAAGTTAAAT
    TCTCTGCAGC TTCAAGCCTT GTTACAAAAT TACCACTGTG CACCCGATGA GCCTTTTATT
    CCTACGGATC TCATAGAAAA TGTTGTGGCT GTTGCTGAGA ACACAGCTGA TGAGCTCGCC
    CGCAGTGATG GCAGAGACGT GCAGCTGGAG GAAGACCCAG ACCTACAGCT GCCTTTTCTT
    CTGCCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGTAGATTGG TTCCTCACAC ACGTTCACCA
    GGTACCTGGA CAATATATTT TGAAGGAGCA GATTATGAAA GTCACCTTAT GCGTGAGAAC
    GCAGAACTGG CCCAGCCTCT GAGGAAGGAG CCTGAGATAA TCACTGTGAC TCTGAAGAAG
    CAGAATGGAA TGGGCCTCAG CATCGTTGCA GCCAAGGGTG CTGGCCAGGA TAAACTGGGA
    ATCTACGTTA AGTCGGTTGT CAAAGGAGGT GCTGCAGATG TGGATGGACG ATTAGCTGCT
    GGTGACCAAC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTTTCTCA GGAAAGGGCA
    GCAGAACTCA TGACAAGAAC CAGTTCCGTG GTTACACTGG AAGTTGCAAA GCAAGGTGCC
    ATCTATCACG GCCTGGCCAC CCTCCTTAAC CAGCCGTCCC CCATGATGCA AAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCAGACCA AAGAGTGAAG GTTTTGAGCT CTATAATAAT
    TCAGCTCAAA ATGGCTCCCC AGAGAGTCCT CAGATGCCTT GGACAGAGTA CAGTGAGCCA
    AAGAAACTGC CTGGGGATGA CAGGCTGATG AAAAACAGAG CGGACCACCG TTCCAGCCCC
    AATGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGGGTC CATATACATC TGGAACAGCA
    GCTAAGATAA CATCTGTCTC CACTGGAAAC CTCTGCACTG AGGAGCAGTC CCCTCCACCC
    AGACCTGAAG CCTATCCTAT CCCTACTCAG ACATACACCA GAGAGTATTT TACCTTTCCA
    GCTTCAAAGT CGCAGGATCG GATGGCTCCT CCTCAGAGCC AGTGGCCAAA TTATGAGGAA
    AAACCACATG TGCACACAGA AAGTAATCAT TCCAGTATTG CAATCCAGCG TGTTACCCGT
    TCCCAGGAAG AGCTTCGGGA AGAAAAGGTT TACCAACTTG AGCGGCATCG AGTAGAGGCA
    GGCATGGATC GCAAGTGTGA CAGTGATATG TGGATCAATC AGAGCTCCTC AGTGGAATCC
    AGCACATCCA GCCAGGAACA CCTGAACCAT TCTTCCAAGT CAGTTACCCC TGCTTCCACT
    CTGACCAAGA GTGGTCCTGG CCGTTGGAAA ACACCAGCAG CGGTGCTGCC CACCCCTGTG
    GCTGTCTCTC AGCCCATTCG AACAGACCTC CCTCCACCAC CACCTCCACC GCCGGTCCAC
    TACACCAGTG AGTTTGATGG TATTCCAATG GATTTGCCCC TCCCACCTCC TCCTGCCAAC
    CAGGCAGGAC CCCAGTCTGC TCAGGTGGCT GCTGCAGAGT GGAAAAAGAG AGAGGAGCAC
    CAGAGGTGGT ATGAGAAAGA GAAGGCCCGC CTGGAGGAAG AACGAGAGAG AAAGCGCAGG
    GAGCAGGAGC GGAAGTTGGG GCAGATGCGC TCTCAGACCC TGAACCCTGC TTCCTTCTCA
    CCTTTGGCCA CACAGGCCAA GCCAGAAAAG CCTTCCACAC TGCAGAGGCC CCAAGAAACA
    GTCATTCGGG AGCTTCAGCC CCAGCAGCAG CCCCGCACGA TCGAGCGCAA GGATCTGCAG
    TATATAACCA TCAGTAAAGA GGAGCTGTCC TCTGGTGATA GTCTGTCTCC AGACCCCTGG
    AAACGAGATG CCAGGGAGAA GCTGGAGAAG CAACAGCAAA TGCACATTGT GGACATGCTA
    AGCAAGGAGA TCCACGAGCT GCAAAACAAG GTGGACCGTA CTGCGGAGGA GAGTGACCGC
    CTGCGGAAGC TTATGCTGGA GTGGCAGTTC CAGAAGAGAC TACAGGAGTC CAAACAGAAG
    GATGAGGATG ATGACGAAGA GGAAGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGT
    CTGGAGGCCG AGCGGAGAGC CAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG
    ATGCAGGATG AGGAGCGCAG GCGCCAGCAA CAGTTGGAAG AGATGCGGAA ACGAGAAGCA
    GAAGATCGAG TAAGACAAGA GGAAGATGGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA
    GATGCTGAAG AAAAGAGGCG ACAGGAGGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG
    CGCCGACAGC ACGAAGAGGC AGCACGCAGG CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC
    CGACCTCCAC TTCCACGGGA CTATGAGCCC CCGTCCTTGT CCTCAGCACC CTGTGCCCCT
    CCTCCCCCAC CTCAGCGAAA CGCATCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG
    CTGTTCACTG CCAAGTTTGT TGCGTATGAT GAGGAGGAGG AGGACTACGG CCCAGCAGGT
    CAGGATAAGT ACCCCAGCAC ACGGAAGTCT CATGGGCACC TTCCTCTTGC CACCCTTAAG
    CCGCAGCAGC CCCCCTGCCA GCCACGCCCA GCCTCGGATG GCATCTCCCT CTGCAACTCA
    TTCCAGCCAC CCTTGGCCAA AGCCAACAGT ACAGCTTACA AAGCAGGCCA GCCCCCGCCC
    CTGCCAGGCA AACCAAGCCT GTACCGTCCT GGCCACTGGA AAGGTCGAGG ACCAAACTCT
    TACTCGGGAT CTGCAGGCAC AGCTGTGGGA GCCTATGATG CCCCTCGGGA AGCGAGAGAG
    AAACTGACTA GGAGCCAAGA CGCAGACTTA CCTGGCAGTT CTGGAGCCCC TGAAAATCTG
    ACATTTAAAG AGCGTCAGCG CCTTTTTTCA CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA
    GCTTCTCGTA AATTAACAGA GCTCGAGAAT GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523819.1
    CDS131..5878
    Translation

    Target ORF information:

    RefSeq Version XM_006523756.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523756.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC
    AGCTATCACA CTTACGAAGA TGGCTCTGAC TCCAGAGACA AGCCAAAGTT ATACCGCCTT
    CAATTAAGTG TGACTGAAGT TGGGACAGAG AAATTTGATG ACAATTCTAT CCAGCTGTTT
    GGCCCCGGAA TTCAGCCCCA TCACTGTGAT CTCACCAACA TGGATGGAGT GGTCACTGTG
    ACACCCAGAA GTATGGATGC AGAGACCTAC GTGGATGGTC AGCGCATCTC AGAGACCACA
    ATGCTGCAGA GTGGCATGAG ACTGCAGTTT GGCACCTCGC ATGTGTTTAA GTTTGTGGAC
    CCCATCCAGG ACCACGTTCT TTCCAAGAGA TCTGTGGATG GAGGCCTGAT GGTCAAGGGC
    CCAAGACATA AACCTGGAGC TGTTCAGGAG ACAACCTTTG AATTGGGAGG AGATGTCCAC
    AGTGGGACAG CATTGCCGGC AAGCAGGAGC ACTACTAGAC TGGACAGTGA CAGGGTGTCC
    TCTGCCTCTA GCACAGCTGA GCGAGGGATG GTGAAGCCGA TGATCCGACT GGACCAGGAG
    CAGGAATATC GACGACGAGA AAACAGGACT CAGGATGCTA CTGGGCCTGA ACTGATACTG
    CCTGCCAGCA TTGAATTCAG GGAAAGCTCT GAAGACTCTT TTTTATCTGC CATTATAAAC
    TATACCAATA GTTCTACAGT TCATTTTAAG TTGTCACCTA CATATGTGTT ATACATGGCA
    TGCCGGTATG TATTGTCCAG CCAGCACAGG CCCGACATCA GTCCTACAGA GCGTACACAT
    AAGGCCATTG CTGTTGTCAA CAAGATGGTG AGCATGATGG AAGGGGTCAT CCAGGAAGTA
    GACCAGGTTG ATCAGAAACA GAAGAACATT GCAGGAGCAC TTGCTTTTTG GATGGCAAAT
    GCATCTGAGC TTCTCAACTT CATCAAGCAG GACCGAGACC TTAGTCGGAT CACGCTGGAT
    GCCCAGGATG TCCTAGCACA CTTGGTTCAG ATGGCATTTA AGTACTTGGT TCATTGCCTT
    CAGTCAGAAC TTAATAACTA CATGCCAGCC TTTCTGGATG ATCCTGAGGA GAACAGTCTG
    CAAAGACCAA AAATAGATGA TGTGCTGCAC ACACTCACAG GAGCCATGTC CTTGCTGCGG
    CGCTGCAGAG TCAATGCTGC CTTGACCATC CAGCTCTTCT CTCAGCTTTT CCACTTCATC
    AATATGTGGC TGTTCAACAG ACTGGTGACT GACCCAGATT CAGGCCTGTG CTCCCACTAC
    TGGGGTGCCA TTATCCGCCA GCAGCTGGGG CATATTGAAG CGTGGGCAGA GAAGCAGGGG
    CTAGAGCTGG CAGCTGACTG TCACTTGAGC AGAATTGTAC AGGCTACAAC ATTGCTTACG
    ATGGATAAGT ATGTACCTGA TGACATTCCA AATATAAACA GCACATGCTT TAAGTTAAAT
    TCTCTGCAGC TTCAAGCCTT GTTACAAAAT TACCACTGTG CACCCGATGA GCCTTTTATT
    CCTACGGATC TCATAGAAAA TGTTGTGGCT GTTGCTGAGA ACACAGCTGA TGAGCTCGCC
    CGCAGTGATG GCAGAGACGT GCAGCTGGAG GAAGACCCAG ACCTACAGCT GCCTTTTCTT
    CTGCCAGAAG ATGGCTATTC CTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA
    TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGTAGATTGG TTCCTCACAC ACGTTCACCA
    GGTACCTGGA CAATATATTT TGAAGGAGCA GATTATGAAA GTCACCTTAT GCGTGAGAAC
    GCAGAACTGG CCCAGCCTCT GAGGAAGGAG CCTGAGATAA TCACTGTGAC TCTGAAGAAG
    CAGAATGGAA TGGGCCTCAG CATCGTTGCA GCCAAGGGTG CTGGCCAGGA TAAACTGGGA
    ATCTACGTTA AGTCGGTTGT CAAAGGAGGT GCTGCAGATG TGGATGGACG ATTAGCTGCT
    GGTGACCAAC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTTTCTCA GGAAAGGGCA
    GCAGAACTCA TGACAAGAAC CAGTTCCGTG GTTACACTGG AAGTTGCAAA GCAAGGTGCC
    ATCTATCACG GCCTGGCCAC CCTCCTTAAC CAGCCGTCCC CCATGATGCA AAGAATTTCA
    GATCGTCGTG GCTCAGGTAA ACCCAGACCA AAGAGTGAAG GTTTTGAGCT CTATAATAAT
    TCAGCTCAAA ATGGCTCCCC AGAGAGTCCT CAGATGCCTT GGACAGAGTA CAGTGAGCCA
    AAGAAACTGC CTGGGGATGA CAGGCTGATG AAAAACAGAG CGGACCACCG TTCCAGCCCC
    AATGTGGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGGGTC CATATACATC TGGAACAGCA
    GCTAAGATAA CATCTGTCTC CACTGGAAAC CTCTGCACTG AGGAGCAGTC CCCTCCACCC
    AGACCTGAAG CCTATCCTAT CCCTACTCAG ACATACACCA GAGAGTATTT TACCTTTCCA
    GCTTCAAAGT CGCAGGATCG GATGGCTCCT CCTCAGAGCC AGTGGCCAAA TTATGAGGAA
    AAACCACATG TGCACACAGA AAGTAATCAT TCCAGTATTG CAATCCAGCG TGTTACCCGT
    TCCCAGGAAG AGCTTCGGGA AGAAAAGGTT TACCAACTTG AGCGGCATCG AGTAGAGGCA
    GGCATGGATC GCAAGTGTGA CAGTGATATG TGGATCAATC AGAGCTCCTC AGTGGAATCC
    AGCACATCCA GCCAGGAACA CCTGAACCAT TCTTCCAAGT CAGTTACCCC TGCTTCCACT
    CTGACCAAGA GTGGTCCTGG CCGTTGGAAA ACACCAGCAG CGGTGCTGCC CACCCCTGTG
    GCTGTCTCTC AGCCCATTCG AACAGACCTC CCTCCACCAC CACCTCCACC GCCGGTCCAC
    TACACCAGTG AGTTTGATGG TATTCCAATG GATTTGCCCC TCCCACCTCC TCCTGCCAAC
    CAGGCAGGAC CCCAGTCTGC TCAGGTGGCT GCTGCAGAGT GGAAAAAGAG AGAGGAGCAC
    CAGAGGTGGT ATGAGAAAGA GAAGGCCCGC CTGGAGGAAG AACGAGAGAG AAAGCGCAGG
    GAGCAGGAGC GGAAGTTGGG GCAGATGCGC TCTCAGACCC TGAACCCTGC TTCCTTCTCA
    CCTTTGGCCA CACAGGCCAA GCCAGAAAAG CCTTCCACAC TGCAGAGGCC CCAAGAAACA
    GTCATTCGGG AGCTTCAGCC CCAGCAGCAG CCCCGCACGA TCGAGCGCAA GGATCTGCAG
    TATATAACCA TCAGTAAAGA GGAGCTGTCC TCTGGTGATA GTCTGTCTCC AGACCCCTGG
    AAACGAGATG CCAGGGAGAA GCTGGAGAAG CAACAGCAAA TGCACATTGT GGACATGCTA
    AGCAAGGAGA TCCACGAGCT GCAAAACAAG GTGGACCGTA CTGCGGAGGA GAGTGACCGC
    CTGCGGAAGC TTATGCTGGA GTGGCAGTTC CAGAAGAGAC TACAGGAGTC CAAACAGAAG
    GATGAGGATG ATGACGAAGA GGAAGATGAT GATGTGGACA CCATGCTGAT CATGCAGCGT
    CTGGAGGCCG AGCGGAGAGC CAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG
    ATGCAGGATG AGGAGCGCAG GCGCCAGCAA CAGTTGGAAG AGATGCGGAA ACGAGAAGCA
    GAAGATCGAG TAAGACAAGA GGAAGATGGA CGCCATCAGG AGGAGGAGCG AGTAAAGAGA
    GATGCTGAAG AAAAGAGGCG ACAGGAGGAA GGGTATTACA GCCGCCTAGA AGCTGAGAGG
    CGCCGACAGC ACGAAGAGGC AGCACGCAGG CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC
    CGACCTCCAC TTCCACGGGA CTATGAGCCC CCGTCCTTGT CCTCAGCACC CTGTGCCCCT
    CCTCCCCCAC CTCAGCGAAA CGCATCCTAC CTCAAAACAC AGGTCCTCTC CCCAGACTCG
    CTGTTCACTG CCAAGTTTGT TGCGTATGAT GAGGAGGAGG AGGACTACGG CCCAGCAGGT
    CAGGATAAGT ACCCCAGCAC ACGGAAGTCT CATGGGCACC TTCCTCTTGC CACCCTTAAG
    CCGCAGCAGC CCCCCTGCCA GCCACGCCCA GCCTCGGATG GCATCTCCCT CTGCAACTCA
    TTCCAGCCAC CCTTGGCCAA AGCCAACAGT ACAGCTTACA AAGCAGGCCA GCCCCCGCCC
    CTGCCAGGCA AACCAAGCCT GTACCGTCCT GGCCACTGGA AAGGTCGAGG ACCAAACTCT
    TACTCGGGAT CTGCAGGCAC AGCTGTGGGA GCCTATGATG CCCCTCGGGA AGCGAGAGAG
    AAACTGACTA GGAGCCAAGA CGCAGACTTA CCTGGCAGTT CTGGAGCCCC TGAAAATCTG
    ACATTTAAAG AGCGTCAGCG CCTTTTTTCA CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA
    GCTTCTCGTA AATTAACAGA GCTCGAGAAT GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53211
    Clone ID Related Accession (Same CDS sequence) XM_006523757.2 , XM_006523757.3
    Accession Version XM_006523757.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523757.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGTCAGGA TAAGTACCCC
    AGCACACGGA AGTCTCATGG GCACCTTCCT CTTGCCACCC TTAAGCCGCA GCAGCCCCCC
    TGCCAGCCAC GCCCAGCCTC GGATGGCATC TCCCTCTGCA ACTCATTCCA GCCACCCTTG
    GCCAAAGCCA ACAGTACAGC TTACAAAGCA GGCCAGCCCC CGCCCCTGCC AGGCAAACCA
    AGCCTGTACC GTCCTGGCCA CTGGAAAGGT CGAGGACCAA ACTCTTACTC GGGATCTGCA
    GGCACAGCTG TGGGAGCCTA TGATGCCCCT CGGGAAGCGA GAGAGAAACT GACTAGGAGC
    CAAGACGCAG ACTTACCTGG CAGTTCTGGA GCCCCTGAAA ATCTGACATT TAAAGAGCGT
    CAGCGCCTTT TTTCACAAGG GCAGGATGTG TCTGACAAAG TGAAAGCTTC TCGTAAATTA
    ACAGAGCTCG AGAATGAACT GAACACAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523820.1
    CDS131..5863
    Translation

    Target ORF information:

    RefSeq Version XM_006523757.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523757.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGTCAGGA TAAGTACCCC
    AGCACACGGA AGTCTCATGG GCACCTTCCT CTTGCCACCC TTAAGCCGCA GCAGCCCCCC
    TGCCAGCCAC GCCCAGCCTC GGATGGCATC TCCCTCTGCA ACTCATTCCA GCCACCCTTG
    GCCAAAGCCA ACAGTACAGC TTACAAAGCA GGCCAGCCCC CGCCCCTGCC AGGCAAACCA
    AGCCTGTACC GTCCTGGCCA CTGGAAAGGT CGAGGACCAA ACTCTTACTC GGGATCTGCA
    GGCACAGCTG TGGGAGCCTA TGATGCCCCT CGGGAAGCGA GAGAGAAACT GACTAGGAGC
    CAAGACGCAG ACTTACCTGG CAGTTCTGGA GCCCCTGAAA ATCTGACATT TAAAGAGCGT
    CAGCGCCTTT TTTCACAAGG GCAGGATGTG TCTGACAAAG TGAAAGCTTC TCGTAAATTA
    ACAGAGCTCG AGAATGAACT GAACACAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53212
    Clone ID Related Accession (Same CDS sequence) XM_006523758.2 , XM_006523758.3
    Accession Version XM_006523758.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523821.1
    CDS131..5860
    Translation

    Target ORF information:

    RefSeq Version XM_006523758.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523758.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGAAA
    CAGAAGAACA TTGCAGGAGC ACTTGCTTTT TGGATGGCAA ATGCATCTGA GCTTCTCAAC
    TTCATCAAGC AGGACCGAGA CCTTAGTCGG ATCACGCTGG ATGCCCAGGA TGTCCTAGCA
    CACTTGGTTC AGATGGCATT TAAGTACTTG GTTCATTGCC TTCAGTCAGA ACTTAATAAC
    TACATGCCAG CCTTTCTGGA TGATCCTGAG GAGAACAGTC TGCAAAGACC AAAAATAGAT
    GATGTGCTGC ACACACTCAC AGGAGCCATG TCCTTGCTGC GGCGCTGCAG AGTCAATGCT
    GCCTTGACCA TCCAGCTCTT CTCTCAGCTT TTCCACTTCA TCAATATGTG GCTGTTCAAC
    AGACTGGTGA CTGACCCAGA TTCAGGCCTG TGCTCCCACT ACTGGGGTGC CATTATCCGC
    CAGCAGCTGG GGCATATTGA AGCGTGGGCA GAGAAGCAGG GGCTAGAGCT GGCAGCTGAC
    TGTCACTTGA GCAGAATTGT ACAGGCTACA ACATTGCTTA CGATGGATAA GTATGTACCT
    GATGACATTC CAAATATAAA CAGCACATGC TTTAAGTTAA ATTCTCTGCA GCTTCAAGCC
    TTGTTACAAA ATTACCACTG TGCACCCGAT GAGCCTTTTA TTCCTACGGA TCTCATAGAA
    AATGTTGTGG CTGTTGCTGA GAACACAGCT GATGAGCTCG CCCGCAGTGA TGGCAGAGAC
    GTGCAGCTGG AGGAAGACCC AGACCTACAG CTGCCTTTTC TTCTGCCAGA AGATGGCTAT
    TCCTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC
    CAGAGAGGAT TTTGTAGATT GGTTCCTCAC ACACGTTCAC CAGGTACCTG GACAATATAT
    TTTGAAGGAG CAGATTATGA AAGTCACCTT ATGCGTGAGA ACGCAGAACT GGCCCAGCCT
    CTGAGGAAGG AGCCTGAGAT AATCACTGTG ACTCTGAAGA AGCAGAATGG AATGGGCCTC
    AGCATCGTTG CAGCCAAGGG TGCTGGCCAG GATAAACTGG GAATCTACGT TAAGTCGGTT
    GTCAAAGGAG GTGCTGCAGA TGTGGATGGA CGATTAGCTG CTGGTGACCA ACTCCTCAGT
    GTGGATGGAC GAAGTCTGGT TGGACTTTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA
    ACCAGTTCCG TGGTTACACT GGAAGTTGCA AAGCAAGGTG CCATCTATCA CGGCCTGGCC
    ACCCTCCTTA ACCAGCCGTC CCCCATGATG CAAAGAATTT CAGATCGTCG TGGCTCAGGT
    AAACCCAGAC CAAAGAGTGA AGGTTTTGAG CTCTATAATA ATTCAGCTCA AAATGGCTCC
    CCAGAGAGTC CTCAGATGCC TTGGACAGAG TACAGTGAGC CAAAGAAACT GCCTGGGGAT
    GACAGGCTGA TGAAAAACAG AGCGGACCAC CGTTCCAGCC CCAATGTGGC AAATCAGCCT
    CCTAGCCCTG GAGGGAAGGG TCCATATACA TCTGGAACAG CAGCTAAGAT AACATCTGTC
    TCCACTGGAA ACCTCTGCAC TGAGGAGCAG TCCCCTCCAC CCAGACCTGA AGCCTATCCT
    ATCCCTACTC AGACATACAC CAGAGAGTAT TTTACCTTTC CAGCTTCAAA GTCGCAGGAT
    CGGATGGCTC CTCCTCAGAG CCAGTGGCCA AATTATGAGG AAAAACCACA TGTGCACACA
    GAAAGTAATC ATTCCAGTAT TGCAATCCAG CGTGTTACCC GTTCCCAGGA AGAGCTTCGG
    GAAGAAAAGG TTTACCAACT TGAGCGGCAT CGAGTAGAGG CAGGCATGGA TCGCAAGTGT
    GACAGTGATA TGTGGATCAA TCAGAGCTCC TCAGTGGAAT CCAGCACATC CAGCCAGGAA
    CACCTGAACC ATTCTTCCAA GTCAGTTACC CCTGCTTCCA CTCTGACCAA GAGTGGTCCT
    GGCCGTTGGA AAACACCAGC AGCGGTGCTG CCCACCCCTG TGGCTGTCTC TCAGCCCATT
    CGAACAGACC TCCCTCCACC ACCACCTCCA CCGCCGGTCC ACTACACCAG TGAGTTTGAT
    GGTATTCCAA TGGATTTGCC CCTCCCACCT CCTCCTGCCA ACCAGGCAGG ACCCCAGTCT
    GCTCAGGTGG CTGCTGCAGA GTGGAAAAAG AGAGAGGAGC ACCAGAGGTG GTATGAGAAA
    GAGAAGGCCC GCCTGGAGGA AGAACGAGAG AGAAAGCGCA GGGAGCAGGA GCGGAAGTTG
    GGGCAGATGC GCTCTCAGAC CCTGAACCCT GCTTCCTTCT CACCTTTGGC CACACAGGCC
    AAGCCAGAAA AGCCTTCCAC ACTGCAGAGG CCCCAAGAAA CAGTCATTCG GGAGCTTCAG
    CCCCAGCAGC AGCCCCGCAC GATCGAGCGC AAGGATCTGC AGTATATAAC CATCAGTAAA
    GAGGAGCTGT CCTCTGGTGA TAGTCTGTCT CCAGACCCCT GGAAACGAGA TGCCAGGGAG
    AAGCTGGAGA AGCAACAGCA AATGCACATT GTGGACATGC TAAGCAAGGA GATCCACGAG
    CTGCAAAACA AGGTGGACCG TACTGCGGAG GAGAGTGACC GCCTGCGGAA GCTTATGCTG
    GAGTGGCAGT TCCAGAAGAG ACTACAGGAG TCCAAACAGA AGGATGAGGA TGATGACGAA
    GAGGAAGATG ATGATGTGGA CACCATGCTG ATCATGCAGC GTCTGGAGGC CGAGCGGAGA
    GCCAGGCAGA CTGCTATGCC AGCAATCTCT GTTCTAGATT TGATGCAGGA TGAGGAGCGC
    AGGCGCCAGC AACAGTTGGA AGAGATGCGG AAACGAGAAG CAGAAGATCG AGTAAGACAA
    GAGGAAGATG GACGCCATCA GGAGGAGGAG CGAGTAAAGA GAGATGCTGA AGAAAAGAGG
    CGACAGGAGG AAGGGTATTA CAGCCGCCTA GAAGCTGAGA GGCGCCGACA GCACGAAGAG
    GCAGCACGCA GGCTGCTGGA GCCTGAAGAG CCCGGGCTGA GCCGACCTCC ACTTCCACGG
    GACTATGAGC CCCCGTCCTT GTCCTCAGCA CCCTGTGCCC CTCCTCCCCC ACCTCAGCGA
    AACGCATCCT ACCTCAAAAC ACAGGTCCTC TCCCCAGACT CGCTGTTCAC TGCCAAGTTT
    GTTGCGTATG ATGAGGAGGA GGAGGACTAC GGCCCAGCAG GTCAGGATAA GTACCCCAGC
    ACACGGAAGT CTCATGGGCA CCTTCCTCTT GCCACCCTTA AGCCGCAGCA GCCCCCCTGC
    CAGCCACGCC CAGCCTCGGA TGGCATCTCC CTCTGCAACT CATTCCAGCC ACCCTTGGCC
    AAAGCCAACA GTACAGCTTA CAAAGCAGGC CAGCCCCCGC CCCTGCCAGG CAAACCAAGC
    CTGTACCGTC CTGGCCACTG GAAAGGTCGA GGACCAAACT CTTACTCGGG ATCTGCAGGC
    ACAGCTGTGG GAGCCTATGA TGCCCCTCGG GAAGCGAGAG AGAAACTGAC TAGGAGCCAA
    GACGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAATC TGACATTTAA AGAGCGTCAG
    CGCCTTTTTT CACAAGGGCA GGATGTGTCT GACAAAGTGA AAGCTTCTCG TAAATTAACA
    GAGCTCGAGA ATGAACTGAA CACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53213
    Clone ID Related Accession (Same CDS sequence) XM_006523759.2 , XM_006523759.3
    Accession Version XM_006523759.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGT CAGGATAAGT ACCCCAGCAC ACGGAAGTCT
    CATGGGCACC TTCCTCTTGC CACCCTTAAG CCGCAGCAGC CCCCCTGCCA GCCACGCCCA
    GCCTCGGATG GCATCTCCCT CTGCAACTCA TTCCAGCCAC CCTTGGCCAA AGCCAACAGT
    ACAGCTTACA AAGCAGGCCA GCCCCCGCCC CTGCCAGGCA AACCAAGCCT GTACCGTCCT
    GGCCACTGGA AAGGTCGAGG ACCAAACTCT TACTCGGGAT CTGCAGGCAC AGCTGTGGGA
    GCCTATGATG CCCCTCGGGA AGCGAGAGAG AAACTGACTA GGAGCCAAGA CGCAGACTTA
    CCTGGCAGTT CTGGAGCCCC TGAAAATCTG ACATTTAAAG AGCGTCAGCG CCTTTTTTCA
    CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA GCTTCTCGTA AATTAACAGA GCTCGAGAAT
    GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523822.1
    CDS131..5848
    Translation

    Target ORF information:

    RefSeq Version XM_006523759.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523759.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGT CAGGATAAGT ACCCCAGCAC ACGGAAGTCT
    CATGGGCACC TTCCTCTTGC CACCCTTAAG CCGCAGCAGC CCCCCTGCCA GCCACGCCCA
    GCCTCGGATG GCATCTCCCT CTGCAACTCA TTCCAGCCAC CCTTGGCCAA AGCCAACAGT
    ACAGCTTACA AAGCAGGCCA GCCCCCGCCC CTGCCAGGCA AACCAAGCCT GTACCGTCCT
    GGCCACTGGA AAGGTCGAGG ACCAAACTCT TACTCGGGAT CTGCAGGCAC AGCTGTGGGA
    GCCTATGATG CCCCTCGGGA AGCGAGAGAG AAACTGACTA GGAGCCAAGA CGCAGACTTA
    CCTGGCAGTT CTGGAGCCCC TGAAAATCTG ACATTTAAAG AGCGTCAGCG CCTTTTTTCA
    CAAGGGCAGG ATGTGTCTGA CAAAGTGAAA GCTTCTCGTA AATTAACAGA GCTCGAGAAT
    GAACTGAACA CAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53214
    Clone ID Related Accession (Same CDS sequence) XM_006523760.2 , XM_006523760.3
    Accession Version XM_006523760.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGAGCA
    CTTGCTTTTT GGATGGCAAA TGCATCTGAG CTTCTCAACT TCATCAAGCA GGACCGAGAC
    CTTAGTCGGA TCACGCTGGA TGCCCAGGAT GTCCTAGCAC ACTTGGTTCA GATGGCATTT
    AAGTACTTGG TTCATTGCCT TCAGTCAGAA CTTAATAACT ACATGCCAGC CTTTCTGGAT
    GATCCTGAGG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACACTCACA
    GGAGCCATGT CCTTGCTGCG GCGCTGCAGA GTCAATGCTG CCTTGACCAT CCAGCTCTTC
    TCTCAGCTTT TCCACTTCAT CAATATGTGG CTGTTCAACA GACTGGTGAC TGACCCAGAT
    TCAGGCCTGT GCTCCCACTA CTGGGGTGCC ATTATCCGCC AGCAGCTGGG GCATATTGAA
    GCGTGGGCAG AGAAGCAGGG GCTAGAGCTG GCAGCTGACT GTCACTTGAG CAGAATTGTA
    CAGGCTACAA CATTGCTTAC GATGGATAAG TATGTACCTG ATGACATTCC AAATATAAAC
    AGCACATGCT TTAAGTTAAA TTCTCTGCAG CTTCAAGCCT TGTTACAAAA TTACCACTGT
    GCACCCGATG AGCCTTTTAT TCCTACGGAT CTCATAGAAA ATGTTGTGGC TGTTGCTGAG
    AACACAGCTG ATGAGCTCGC CCGCAGTGAT GGCAGAGACG TGCAGCTGGA GGAAGACCCA
    GACCTACAGC TGCCTTTTCT TCTGCCAGAA GATGGCTATT CCTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGTAGATTG
    GTTCCTCACA CACGTTCACC AGGTACCTGG ACAATATATT TTGAAGGAGC AGATTATGAA
    AGTCACCTTA TGCGTGAGAA CGCAGAACTG GCCCAGCCTC TGAGGAAGGA GCCTGAGATA
    ATCACTGTGA CTCTGAAGAA GCAGAATGGA ATGGGCCTCA GCATCGTTGC AGCCAAGGGT
    GCTGGCCAGG ATAAACTGGG AATCTACGTT AAGTCGGTTG TCAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GATTAGCTGC TGGTGACCAA CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTTTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CCAGTTCCGT GGTTACACTG
    GAAGTTGCAA AGCAAGGTGC CATCTATCAC GGCCTGGCCA CCCTCCTTAA CCAGCCGTCC
    CCCATGATGC AAAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCAGACC AAAGAGTGAA
    GGTTTTGAGC TCTATAATAA TTCAGCTCAA AATGGCTCCC CAGAGAGTCC TCAGATGCCT
    TGGACAGAGT ACAGTGAGCC AAAGAAACTG CCTGGGGATG ACAGGCTGAT GAAAAACAGA
    GCGGACCACC GTTCCAGCCC CAATGTGGCA AATCAGCCTC CTAGCCCTGG AGGGAAGGGT
    CCATATACAT CTGGAACAGC AGCTAAGATA ACATCTGTCT CCACTGGAAA CCTCTGCACT
    GAGGAGCAGT CCCCTCCACC CAGACCTGAA GCCTATCCTA TCCCTACTCA GACATACACC
    AGAGAGTATT TTACCTTTCC AGCTTCAAAG TCGCAGGATC GGATGGCTCC TCCTCAGAGC
    CAGTGGCCAA ATTATGAGGA AAAACCACAT GTGCACACAG AAAGTAATCA TTCCAGTATT
    GCAATCCAGC GTGTTACCCG TTCCCAGGAA GAGCTTCGGG AAGAAAAGGT TTACCAACTT
    GAGCGGCATC GAGTAGAGGC AGGCATGGAT CGCAAGTGTG ACAGTGATAT GTGGATCAAT
    CAGAGCTCCT CAGTGGAATC CAGCACATCC AGCCAGGAAC ACCTGAACCA TTCTTCCAAG
    TCAGTTACCC CTGCTTCCAC TCTGACCAAG AGTGGTCCTG GCCGTTGGAA AACACCAGCA
    GCGGTGCTGC CCACCCCTGT GGCTGTCTCT CAGCCCATTC GAACAGACCT CCCTCCACCA
    CCACCTCCAC CGCCGGTCCA CTACACCAGT GAGTTTGATG GTATTCCAAT GGATTTGCCC
    CTCCCACCTC CTCCTGCCAA CCAGGCAGGA CCCCAGTCTG CTCAGGTGGC TGCTGCAGAG
    TGGAAAAAGA GAGAGGAGCA CCAGAGGTGG TATGAGAAAG AGAAGGCCCG CCTGGAGGAA
    GAACGAGAGA GAAAGCGCAG GGAGCAGGAG CGGAAGTTGG GGCAGATGCG CTCTCAGACC
    CTGAACCCTG CTTCCTTCTC ACCTTTGGCC ACACAGGCCA AGCCAGAAAA GCCTTCCACA
    CTGCAGAGGC CCCAAGAAAC AGTCATTCGG GAGCTTCAGC CCCAGCAGCA GCCCCGCACG
    ATCGAGCGCA AGGATCTGCA GTATATAACC ATCAGTAAAG AGGAGCTGTC CTCTGGTGAT
    AGTCTGTCTC CAGACCCCTG GAAACGAGAT GCCAGGGAGA AGCTGGAGAA GCAACAGCAA
    ATGCACATTG TGGACATGCT AAGCAAGGAG ATCCACGAGC TGCAAAACAA GGTGGACCGT
    ACTGCGGAGG AGAGTGACCG CCTGCGGAAG CTTATGCTGG AGTGGCAGTT CCAGAAGAGA
    CTACAGGAGT CCAAACAGAA GGATGAGGAT GATGACGAAG AGGAAGATGA TGATGTGGAC
    ACCATGCTGA TCATGCAGCG TCTGGAGGCC GAGCGGAGAG CCAGGCAGAC TGCTATGCCA
    GCAATCTCTG TTCTAGATTT GATGCAGGAT GAGGAGCGCA GGCGCCAGCA ACAGTTGGAA
    GAGATGCGGA AACGAGAAGC AGAAGATCGA GTAAGACAAG AGGAAGATGG ACGCCATCAG
    GAGGAGGAGC GAGTAAAGAG AGATGCTGAA GAAAAGAGGC GACAGGAGGA AGGGTATTAC
    AGCCGCCTAG AAGCTGAGAG GCGCCGACAG CACGAAGAGG CAGCACGCAG GCTGCTGGAG
    CCTGAAGAGC CCGGGCTGAG CCGACCTCCA CTTCCACGGG ACTATGAGCC CCCGTCCTTG
    TCCTCAGCAC CCTGTGCCCC TCCTCCCCCA CCTCAGCGAA ACGCATCCTA CCTCAAAACA
    CAGGTCCTCT CCCCAGACTC GCTGTTCACT GCCAAGTTTG TTGCGTATGA TGAGGAGGAG
    GAGGACTACG GCCCAGCAGG TCAGGATAAG TACCCCAGCA CACGGAAGTC TCATGGGCAC
    CTTCCTCTTG CCACCCTTAA GCCGCAGCAG CCCCCCTGCC AGCCACGCCC AGCCTCGGAT
    GGCATCTCCC TCTGCAACTC ATTCCAGCCA CCCTTGGCCA AAGCCAACAG TACAGCTTAC
    AAAGCAGGCC AGCCCCCGCC CCTGCCAGGC AAACCAAGCC TGTACCGTCC TGGCCACTGG
    AAAGGTCGAG GACCAAACTC TTACTCGGGA TCTGCAGGCA CAGCTGTGGG AGCCTATGAT
    GCCCCTCGGG AAGCGAGAGA GAAACTGACT AGGAGCCAAG ACGCAGACTT ACCTGGCAGT
    TCTGGAGCCC CTGAAAATCT GACATTTAAA GAGCGTCAGC GCCTTTTTTC ACAAGGGCAG
    GATGTGTCTG ACAAAGTGAA AGCTTCTCGT AAATTAACAG AGCTCGAGAA TGAACTGAAC
    ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523823.1
    CDS131..5839
    Translation

    Target ORF information:

    RefSeq Version XM_006523760.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523760.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG ATGGCTCTGA CTCCAGAGAC
    AAGCCAAAGT TATACCGCCT TCAATTAAGT GTGACTGAAG TTGGGACAGA GAAATTTGAT
    GACAATTCTA TCCAGCTGTT TGGCCCCGGA ATTCAGCCCC ATCACTGTGA TCTCACCAAC
    ATGGATGGAG TGGTCACTGT GACACCCAGA AGTATGGATG CAGAGACCTA CGTGGATGGT
    CAGCGCATCT CAGAGACCAC AATGCTGCAG AGTGGCATGA GACTGCAGTT TGGCACCTCG
    CATGTGTTTA AGTTTGTGGA CCCCATCCAG GACCACGTTC TTTCCAAGAG ATCTGTGGAT
    GGAGGCCTGA TGGTCAAGGG CCCAAGACAT AAACCTGGAG CTGTTCAGGA GACAACCTTT
    GAATTGGGAG GAGATGTCCA CAGTGGGACA GCATTGCCGG CAAGCAGGAG CACTACTAGA
    CTGGACAGTG ACAGGGTGTC CTCTGCCTCT AGCACAGCTG AGCGAGGGAT GGTGAAGCCG
    ATGATCCGAC TGGACCAGGA GCAGGAATAT CGACGACGAG AAAACAGGAC TCAGGATGCT
    ACTGGGCCTG AACTGATACT GCCTGCCAGC ATTGAATTCA GGGAAAGCTC TGAAGACTCT
    TTTTTATCTG CCATTATAAA CTATACCAAT AGTTCTACAG TTCATTTTAA GTTGTCACCT
    ACATATGTGT TATACATGGC ATGCCGGTAT GTATTGTCCA GCCAGCACAG GCCCGACATC
    AGTCCTACAG AGCGTACACA TAAGGCCATT GCTGTTGTCA ACAAGATGGT GAGCATGATG
    GAAGGGGTCA TCCAGGAAGT AGACCAGGTT GATCAGAAAC AGAAGAACAT TGCAGGAGCA
    CTTGCTTTTT GGATGGCAAA TGCATCTGAG CTTCTCAACT TCATCAAGCA GGACCGAGAC
    CTTAGTCGGA TCACGCTGGA TGCCCAGGAT GTCCTAGCAC ACTTGGTTCA GATGGCATTT
    AAGTACTTGG TTCATTGCCT TCAGTCAGAA CTTAATAACT ACATGCCAGC CTTTCTGGAT
    GATCCTGAGG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACACTCACA
    GGAGCCATGT CCTTGCTGCG GCGCTGCAGA GTCAATGCTG CCTTGACCAT CCAGCTCTTC
    TCTCAGCTTT TCCACTTCAT CAATATGTGG CTGTTCAACA GACTGGTGAC TGACCCAGAT
    TCAGGCCTGT GCTCCCACTA CTGGGGTGCC ATTATCCGCC AGCAGCTGGG GCATATTGAA
    GCGTGGGCAG AGAAGCAGGG GCTAGAGCTG GCAGCTGACT GTCACTTGAG CAGAATTGTA
    CAGGCTACAA CATTGCTTAC GATGGATAAG TATGTACCTG ATGACATTCC AAATATAAAC
    AGCACATGCT TTAAGTTAAA TTCTCTGCAG CTTCAAGCCT TGTTACAAAA TTACCACTGT
    GCACCCGATG AGCCTTTTAT TCCTACGGAT CTCATAGAAA ATGTTGTGGC TGTTGCTGAG
    AACACAGCTG ATGAGCTCGC CCGCAGTGAT GGCAGAGACG TGCAGCTGGA GGAAGACCCA
    GACCTACAGC TGCCTTTTCT TCTGCCAGAA GATGGCTATT CCTGTGATGT TGTCAGAAAC
    ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGTAGATTG
    GTTCCTCACA CACGTTCACC AGGTACCTGG ACAATATATT TTGAAGGAGC AGATTATGAA
    AGTCACCTTA TGCGTGAGAA CGCAGAACTG GCCCAGCCTC TGAGGAAGGA GCCTGAGATA
    ATCACTGTGA CTCTGAAGAA GCAGAATGGA ATGGGCCTCA GCATCGTTGC AGCCAAGGGT
    GCTGGCCAGG ATAAACTGGG AATCTACGTT AAGTCGGTTG TCAAAGGAGG TGCTGCAGAT
    GTGGATGGAC GATTAGCTGC TGGTGACCAA CTCCTCAGTG TGGATGGACG AAGTCTGGTT
    GGACTTTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CCAGTTCCGT GGTTACACTG
    GAAGTTGCAA AGCAAGGTGC CATCTATCAC GGCCTGGCCA CCCTCCTTAA CCAGCCGTCC
    CCCATGATGC AAAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCAGACC AAAGAGTGAA
    GGTTTTGAGC TCTATAATAA TTCAGCTCAA AATGGCTCCC CAGAGAGTCC TCAGATGCCT
    TGGACAGAGT ACAGTGAGCC AAAGAAACTG CCTGGGGATG ACAGGCTGAT GAAAAACAGA
    GCGGACCACC GTTCCAGCCC CAATGTGGCA AATCAGCCTC CTAGCCCTGG AGGGAAGGGT
    CCATATACAT CTGGAACAGC AGCTAAGATA ACATCTGTCT CCACTGGAAA CCTCTGCACT
    GAGGAGCAGT CCCCTCCACC CAGACCTGAA GCCTATCCTA TCCCTACTCA GACATACACC
    AGAGAGTATT TTACCTTTCC AGCTTCAAAG TCGCAGGATC GGATGGCTCC TCCTCAGAGC
    CAGTGGCCAA ATTATGAGGA AAAACCACAT GTGCACACAG AAAGTAATCA TTCCAGTATT
    GCAATCCAGC GTGTTACCCG TTCCCAGGAA GAGCTTCGGG AAGAAAAGGT TTACCAACTT
    GAGCGGCATC GAGTAGAGGC AGGCATGGAT CGCAAGTGTG ACAGTGATAT GTGGATCAAT
    CAGAGCTCCT CAGTGGAATC CAGCACATCC AGCCAGGAAC ACCTGAACCA TTCTTCCAAG
    TCAGTTACCC CTGCTTCCAC TCTGACCAAG AGTGGTCCTG GCCGTTGGAA AACACCAGCA
    GCGGTGCTGC CCACCCCTGT GGCTGTCTCT CAGCCCATTC GAACAGACCT CCCTCCACCA
    CCACCTCCAC CGCCGGTCCA CTACACCAGT GAGTTTGATG GTATTCCAAT GGATTTGCCC
    CTCCCACCTC CTCCTGCCAA CCAGGCAGGA CCCCAGTCTG CTCAGGTGGC TGCTGCAGAG
    TGGAAAAAGA GAGAGGAGCA CCAGAGGTGG TATGAGAAAG AGAAGGCCCG CCTGGAGGAA
    GAACGAGAGA GAAAGCGCAG GGAGCAGGAG CGGAAGTTGG GGCAGATGCG CTCTCAGACC
    CTGAACCCTG CTTCCTTCTC ACCTTTGGCC ACACAGGCCA AGCCAGAAAA GCCTTCCACA
    CTGCAGAGGC CCCAAGAAAC AGTCATTCGG GAGCTTCAGC CCCAGCAGCA GCCCCGCACG
    ATCGAGCGCA AGGATCTGCA GTATATAACC ATCAGTAAAG AGGAGCTGTC CTCTGGTGAT
    AGTCTGTCTC CAGACCCCTG GAAACGAGAT GCCAGGGAGA AGCTGGAGAA GCAACAGCAA
    ATGCACATTG TGGACATGCT AAGCAAGGAG ATCCACGAGC TGCAAAACAA GGTGGACCGT
    ACTGCGGAGG AGAGTGACCG CCTGCGGAAG CTTATGCTGG AGTGGCAGTT CCAGAAGAGA
    CTACAGGAGT CCAAACAGAA GGATGAGGAT GATGACGAAG AGGAAGATGA TGATGTGGAC
    ACCATGCTGA TCATGCAGCG TCTGGAGGCC GAGCGGAGAG CCAGGCAGAC TGCTATGCCA
    GCAATCTCTG TTCTAGATTT GATGCAGGAT GAGGAGCGCA GGCGCCAGCA ACAGTTGGAA
    GAGATGCGGA AACGAGAAGC AGAAGATCGA GTAAGACAAG AGGAAGATGG ACGCCATCAG
    GAGGAGGAGC GAGTAAAGAG AGATGCTGAA GAAAAGAGGC GACAGGAGGA AGGGTATTAC
    AGCCGCCTAG AAGCTGAGAG GCGCCGACAG CACGAAGAGG CAGCACGCAG GCTGCTGGAG
    CCTGAAGAGC CCGGGCTGAG CCGACCTCCA CTTCCACGGG ACTATGAGCC CCCGTCCTTG
    TCCTCAGCAC CCTGTGCCCC TCCTCCCCCA CCTCAGCGAA ACGCATCCTA CCTCAAAACA
    CAGGTCCTCT CCCCAGACTC GCTGTTCACT GCCAAGTTTG TTGCGTATGA TGAGGAGGAG
    GAGGACTACG GCCCAGCAGG TCAGGATAAG TACCCCAGCA CACGGAAGTC TCATGGGCAC
    CTTCCTCTTG CCACCCTTAA GCCGCAGCAG CCCCCCTGCC AGCCACGCCC AGCCTCGGAT
    GGCATCTCCC TCTGCAACTC ATTCCAGCCA CCCTTGGCCA AAGCCAACAG TACAGCTTAC
    AAAGCAGGCC AGCCCCCGCC CCTGCCAGGC AAACCAAGCC TGTACCGTCC TGGCCACTGG
    AAAGGTCGAG GACCAAACTC TTACTCGGGA TCTGCAGGCA CAGCTGTGGG AGCCTATGAT
    GCCCCTCGGG AAGCGAGAGA GAAACTGACT AGGAGCCAAG ACGCAGACTT ACCTGGCAGT
    TCTGGAGCCC CTGAAAATCT GACATTTAAA GAGCGTCAGC GCCTTTTTTC ACAAGGGCAG
    GATGTGTCTG ACAAAGTGAA AGCTTCTCGT AAATTAACAG AGCTCGAGAA TGAACTGAAC
    ACAAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53216
    Clone ID Related Accession (Same CDS sequence) XM_006523762.2 , XM_006523762.3
    Accession Version XM_006523762.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGATGCAG
    GATGAGGAGC GCAGGCGCCA GCAACAGTTG GAAGAGATGC GGAAACGAGA AGCAGAAGAT
    CGAGTAAGAC AAGAGGAAGA TGGACGCCAT CAGGAGGAGG AGCGAGTAAA GAGAGATGCT
    GAAGAAAAGA GGCGACAGGA GGAAGGGTAT TACAGCCGCC TAGAAGCTGA GAGGCGCCGA
    CAGCACGAAG AGGCAGCACG CAGGCTGCTG GAGCCTGAAG AGCCCGGGCT GAGCCGACCT
    CCACTTCCAC GGGACTATGA GCCCCCGTCC TTGTCCTCAG CACCCTGTGC CCCTCCTCCC
    CCACCTCAGC GAAACGCATC CTACCTCAAA ACACAGGTCC TCTCCCCAGA CTCGCTGTTC
    ACTGCCAAGT TTGTTGCGTA TGATGAGGAG GAGGAGGACT ACGGCCCAGC AGGTCAGGAT
    AAGTACCCCA GCACACGGAA GTCTCATGGG CACCTTCCTC TTGCCACCCT TAAGCCGCAG
    CAGCCCCCCT GCCAGCCACG CCCAGCCTCG GATGGCATCT CCCTCTGCAA CTCATTCCAG
    CCACCCTTGG CCAAAGCCAA CAGTACAGCT TACAAAGCAG GCCAGCCCCC GCCCCTGCCA
    GGCAAACCAA GCCTGTACCG TCCTGGCCAC TGGAAAGGTC GAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523825.1
    CDS131..5812
    Translation

    Target ORF information:

    RefSeq Version XM_006523762.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523762.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGCA GACTGCTATG CCAGCAATCT CTGTTCTAGA TTTGATGCAG
    GATGAGGAGC GCAGGCGCCA GCAACAGTTG GAAGAGATGC GGAAACGAGA AGCAGAAGAT
    CGAGTAAGAC AAGAGGAAGA TGGACGCCAT CAGGAGGAGG AGCGAGTAAA GAGAGATGCT
    GAAGAAAAGA GGCGACAGGA GGAAGGGTAT TACAGCCGCC TAGAAGCTGA GAGGCGCCGA
    CAGCACGAAG AGGCAGCACG CAGGCTGCTG GAGCCTGAAG AGCCCGGGCT GAGCCGACCT
    CCACTTCCAC GGGACTATGA GCCCCCGTCC TTGTCCTCAG CACCCTGTGC CCCTCCTCCC
    CCACCTCAGC GAAACGCATC CTACCTCAAA ACACAGGTCC TCTCCCCAGA CTCGCTGTTC
    ACTGCCAAGT TTGTTGCGTA TGATGAGGAG GAGGAGGACT ACGGCCCAGC AGGTCAGGAT
    AAGTACCCCA GCACACGGAA GTCTCATGGG CACCTTCCTC TTGCCACCCT TAAGCCGCAG
    CAGCCCCCCT GCCAGCCACG CCCAGCCTCG GATGGCATCT CCCTCTGCAA CTCATTCCAG
    CCACCCTTGG CCAAAGCCAA CAGTACAGCT TACAAAGCAG GCCAGCCCCC GCCCCTGCCA
    GGCAAACCAA GCCTGTACCG TCCTGGCCAC TGGAAAGGTC GAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53217
    Clone ID Related Accession (Same CDS sequence) XM_006523763.2 , XM_006523763.3
    Accession Version XM_006523763.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGACCAA ACTCTTACTC GGGATCTGCA GGCACAGCTG TGGGAGCCTA TGATGCCCCT
    CGGGAAGCGA GAGAGAAACT GACTAGGAGC CAAGACGCAG ACTTACCTGG CAGTTCTGGA
    GCCCCTGAAA ATCTGACATT TAAAGAGCGT CAGCGCCTTT TTTCACAAGG GCAGGATGTG
    TCTGACAAAG TGAAAGCTTC TCGTAAATTA ACAGAGCTCG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523826.1
    CDS131..5653
    Translation

    Target ORF information:

    RefSeq Version XM_006523763.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523763.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA
    GATTTGATGC AGGATGAGGA GCGCAGGCGC CAGCAACAGT TGGAAGAGAT GCGGAAACGA
    GAAGCAGAAG ATCGAGTAAG ACAAGAGGAA GATGGACGCC ATCAGGAGGA GGAGCGAGTA
    AAGAGAGATG CTGAAGAAAA GAGGCGACAG GAGGAAGGGT ATTACAGCCG CCTAGAAGCT
    GAGAGGCGCC GACAGCACGA AGAGGCAGCA CGCAGGCTGC TGGAGCCTGA AGAGCCCGGG
    CTGAGCCGAC CTCCACTTCC ACGGGACTAT GAGCCCCCGT CCTTGTCCTC AGCACCCTGT
    GCCCCTCCTC CCCCACCTCA GCGAAACGCA TCCTACCTCA AAACACAGGT CCTCTCCCCA
    GACTCGCTGT TCACTGCCAA GTTTGTTGCG TATGATGAGG AGGAGGAGGA CTACGGCCCA
    GCAGGACCAA ACTCTTACTC GGGATCTGCA GGCACAGCTG TGGGAGCCTA TGATGCCCCT
    CGGGAAGCGA GAGAGAAACT GACTAGGAGC CAAGACGCAG ACTTACCTGG CAGTTCTGGA
    GCCCCTGAAA ATCTGACATT TAAAGAGCGT CAGCGCCTTT TTTCACAAGG GCAGGATGTG
    TCTGACAAAG TGAAAGCTTC TCGTAAATTA ACAGAGCTCG AGAATGAACT GAACACAAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53218
    Clone ID Related Accession (Same CDS sequence) XM_006523764.3 , XM_006523764.2
    Accession Version XM_006523764.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523827.1
    CDS131..5617
    Translation

    Target ORF information:

    RefSeq Version XM_006523764.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523764.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    GAAGTAGACC AGGTTGATCA GAAACAGAAG AACATTGCAG GAGCACTTGC TTTTTGGATG
    GCAAATGCAT CTGAGCTTCT CAACTTCATC AAGCAGGACC GAGACCTTAG TCGGATCACG
    CTGGATGCCC AGGATGTCCT AGCACACTTG GTTCAGATGG CATTTAAGTA CTTGGTTCAT
    TGCCTTCAGT CAGAACTTAA TAACTACATG CCAGCCTTTC TGGATGATCC TGAGGAGAAC
    AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACAC TCACAGGAGC CATGTCCTTG
    CTGCGGCGCT GCAGAGTCAA TGCTGCCTTG ACCATCCAGC TCTTCTCTCA GCTTTTCCAC
    TTCATCAATA TGTGGCTGTT CAACAGACTG GTGACTGACC CAGATTCAGG CCTGTGCTCC
    CACTACTGGG GTGCCATTAT CCGCCAGCAG CTGGGGCATA TTGAAGCGTG GGCAGAGAAG
    CAGGGGCTAG AGCTGGCAGC TGACTGTCAC TTGAGCAGAA TTGTACAGGC TACAACATTG
    CTTACGATGG ATAAGTATGT ACCTGATGAC ATTCCAAATA TAAACAGCAC ATGCTTTAAG
    TTAAATTCTC TGCAGCTTCA AGCCTTGTTA CAAAATTACC ACTGTGCACC CGATGAGCCT
    TTTATTCCTA CGGATCTCAT AGAAAATGTT GTGGCTGTTG CTGAGAACAC AGCTGATGAG
    CTCGCCCGCA GTGATGGCAG AGACGTGCAG CTGGAGGAAG ACCCAGACCT ACAGCTGCCT
    TTTCTTCTGC CAGAAGATGG CTATTCCTGT GATGTTGTCA GAAACATTCC AAATGGTTTA
    CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGTA GATTGGTTCC TCACACACGT
    TCACCAGGTA CCTGGACAAT ATATTTTGAA GGAGCAGATT ATGAAAGTCA CCTTATGCGT
    GAGAACGCAG AACTGGCCCA GCCTCTGAGG AAGGAGCCTG AGATAATCAC TGTGACTCTG
    AAGAAGCAGA ATGGAATGGG CCTCAGCATC GTTGCAGCCA AGGGTGCTGG CCAGGATAAA
    CTGGGAATCT ACGTTAAGTC GGTTGTCAAA GGAGGTGCTG CAGATGTGGA TGGACGATTA
    GCTGCTGGTG ACCAACTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT TTCTCAGGAA
    AGGGCAGCAG AACTCATGAC AAGAACCAGT TCCGTGGTTA CACTGGAAGT TGCAAAGCAA
    GGTGCCATCT ATCACGGCCT GGCCACCCTC CTTAACCAGC CGTCCCCCAT GATGCAAAGA
    ATTTCAGATC GTCGTGGCTC AGGTAAACCC AGACCAAAGA GTGAAGGTTT TGAGCTCTAT
    AATAATTCAG CTCAAAATGG CTCCCCAGAG AGTCCTCAGA TGCCTTGGAC AGAGTACAGT
    GAGCCAAAGA AACTGCCTGG GGATGACAGG CTGATGAAAA ACAGAGCGGA CCACCGTTCC
    AGCCCCAATG TGGCAAATCA GCCTCCTAGC CCTGGAGGGA AGGGTCCATA TACATCTGGA
    ACAGCAGCTA AGATAACATC TGTCTCCACT GGAAACCTCT GCACTGAGGA GCAGTCCCCT
    CCACCCAGAC CTGAAGCCTA TCCTATCCCT ACTCAGACAT ACACCAGAGA GTATTTTACC
    TTTCCAGCTT CAAAGTCGCA GGATCGGATG GCTCCTCCTC AGAGCCAGTG GCCAAATTAT
    GAGGAAAAAC CACATGTGCA CACAGAAAGT AATCATTCCA GTATTGCAAT CCAGCGTGTT
    ACCCGTTCCC AGGAAGAGCT TCGGGAAGAA AAGGTTTACC AACTTGAGCG GCATCGAGTA
    GAGGCAGGCA TGGATCGCAA GTGTGACAGT GATATGTGGA TCAATCAGAG CTCCTCAGTG
    GAATCCAGCA CATCCAGCCA GGAACACCTG AACCATTCTT CCAAGTCAGT TACCCCTGCT
    TCCACTCTGA CCAAGAGTGG TCCTGGCCGT TGGAAAACAC CAGCAGCGGT GCTGCCCACC
    CCTGTGGCTG TCTCTCAGCC CATTCGAACA GACCTCCCTC CACCACCACC TCCACCGCCG
    GTCCACTACA CCAGTGAGTT TGATGGTATT CCAATGGATT TGCCCCTCCC ACCTCCTCCT
    GCCAACCAGG CAGGACCCCA GTCTGCTCAG GTGGCTGCTG CAGAGTGGAA AAAGAGAGAG
    GAGCACCAGA GGTGGTATGA GAAAGAGAAG GCCCGCCTGG AGGAAGAACG AGAGAGAAAG
    CGCAGGGAGC AGGAGCGGAA GTTGGGGCAG ATGCGCTCTC AGACCCTGAA CCCTGCTTCC
    TTCTCACCTT TGGCCACACA GGCCAAGCCA GAAAAGCCTT CCACACTGCA GAGGCCCCAA
    GAAACAGTCA TTCGGGAGCT TCAGCCCCAG CAGCAGCCCC GCACGATCGA GCGCAAGGAT
    CTGCAGTATA TAACCATCAG TAAAGAGGAG CTGTCCTCTG GTGATAGTCT GTCTCCAGAC
    CCCTGGAAAC GAGATGCCAG GGAGAAGCTG GAGAAGCAAC AGCAAATGCA CATTGTGGAC
    ATGCTAAGCA AGGAGATCCA CGAGCTGCAA AACAAGGTGG ACCGTACTGC GGAGGAGAGT
    GACCGCCTGC GGAAGCTTAT GCTGGAGTGG CAGTTCCAGA AGAGACTACA GGAGTCCAAA
    CAGAAGGATG AGGATGATGA CGAAGAGGAA GATGATGATG TGGACACCAT GCTGATCATG
    CAGCGTCTGG AGGCCGAGCG GAGAGCCAGG ATGCAGGATG AGGAGCGCAG GCGCCAGCAA
    CAGTTGGAAG AGATGCGGAA ACGAGAAGCA GAAGATCGAG TAAGACAAGA GGAAGATGGA
    CGCCATCAGG AGGAGGAGCG AGTAAAGAGA GATGCTGAAG AAAAGAGGCG ACAGGAGGAA
    GGGTATTACA GCCGCCTAGA AGCTGAGAGG CGCCGACAGC ACGAAGAGGC AGCACGCAGG
    CTGCTGGAGC CTGAAGAGCC CGGGCTGAGC CGACCTCCAC TTCCACGGGA CTATGAGCCC
    CCGTCCTTGT CCTCAGCACC CTGTGCCCCT CCTCCCCCAC CTCAGCGAAA CGCATCCTAC
    CTCAAAACAC AGGTCCTCTC CCCAGACTCG CTGTTCACTG CCAAGTTTGT TGCGTATGAT
    GAGGAGGAGG AGGACTACGG CCCAGCAGGA CCAAACTCTT ACTCGGGATC TGCAGGCACA
    GCTGTGGGAG CCTATGATGC CCCTCGGGAA GCGAGAGAGA AACTGACTAG GAGCCAAGAC
    GCAGACTTAC CTGGCAGTTC TGGAGCCCCT GAAAATCTGA CATTTAAAGA GCGTCAGCGC
    CTTTTTTCAC AAGGGCAGGA TGTGTCTGAC AAAGTGAAAG CTTCTCGTAA ATTAACAGAG
    CTCGAGAATG AACTGAACAC AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53219
    Clone ID Related Accession (Same CDS sequence) XM_006523765.3 , XM_006523765.2
    Accession Version XM_006523765.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGATG CAGGATGAGG AGCGCAGGCG CCAGCAACAG
    TTGGAAGAGA TGCGGAAACG AGAAGCAGAA GATCGAGTAA GACAAGAGGA AGATGGACGC
    CATCAGGAGG AGGAGCGAGT AAAGAGAGAT GCTGAAGAAA AGAGGCGACA GGAGGAAGGG
    TATTACAGCC GCCTAGAAGC TGAGAGGCGC CGACAGCACG AAGAGGCAGC ACGCAGGCTG
    CTGGAGCCTG AAGAGCCCGG GCTGAGCCGA CCTCCACTTC CACGGGACTA TGAGCCCCCG
    TCCTTGTCCT CAGCACCCTG TGCCCCTCCT CCCCCACCTC AGCGAAACGC ATCCTACCTC
    AAAACACAGG TCCTCTCCCC AGACTCGCTG TTCACTGCCA AGTTTGTTGC GTATGATGAG
    GAGGAGGAGG ACTACGGCCC AGCAGGACCA AACTCTTACT CGGGATCTGC AGGCACAGCT
    GTGGGAGCCT ATGATGCCCC TCGGGAAGCG AGAGAGAAAC TGACTAGGAG CCAAGACGCA
    GACTTACCTG GCAGTTCTGG AGCCCCTGAA AATCTGACAT TTAAAGAGCG TCAGCGCCTT
    TTTTCACAAG GGCAGGATGT GTCTGACAAA GTGAAAGCTT CTCGTAAATT AACAGAGCTC
    GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523828.1
    CDS131..5614
    Translation

    Target ORF information:

    RefSeq Version XM_006523765.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523765.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT
    CAGAGTAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT
    CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAAAGAGA AGGAGGCATT GAGACAAGCC
    TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAACTCACG ACTGGCTGCT
    GAAGTTTACA AAGACATGCC TGAGACCAGC TTTACTAGGA CCATTTCTAA CCCTGAAGTT
    GTCATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA
    GATGGGCGGC CTGACTCAGG TGGAACACTG AGGATATATG CAGATAGTTT AAAACCAAAT
    ATTCCATACA AGACAATCCT GCTGTCTACT ACCGATACCG CAGACTTTGC TGTAGCCGAA
    TCTTTGGAGA AGTATGGTCT GGAAAAAGAG AATCCCAAGG ACTACTGTAT TGCCCGGGTT
    ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAC
    GATGATGAAT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC
    TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATCCCAAAGA AAATGAAGAA ACATGTTGAA
    GGGAAGTCGT TGAAGGGGAA AGACAGAGCT GACGGGTCTG GCTACGGCTC CGCTCTTCCT
    CCCGAGAAGC TGCCCTACTT AGTAGAGCTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC
    TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC
    CTTCAATTAA GTGTGACTGA AGTTGGGACA GAGAAATTTG ATGACAATTC TATCCAGCTG
    TTTGGCCCCG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT
    GTGACACCCA GAAGTATGGA TGCAGAGACC TACGTGGATG GTCAGCGCAT CTCAGAGACC
    ACAATGCTGC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG
    GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG
    GGCCCAAGAC ATAAACCTGG AGCTGTTCAG GAGACAACCT TTGAATTGGG AGGAGATGTC
    CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GACTGGACAG TGACAGGGTG
    TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CGATGATCCG ACTGGACCAG
    GAGCAGGAAT ATCGACGACG AGAAAACAGG ACTCAGGATG CTACTGGGCC TGAACTGATA
    CTGCCTGCCA GCATTGAATT CAGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA
    AACTATACCA ATAGTTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG
    GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGTACA
    CATAAGGCCA TTGCTGTTGT CAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA
    GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT TTGGATGGCA
    AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATCACGCTG
    GATGCCCAGG ATGTCCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCATTGC
    CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCTGA GGAGAACAGT
    CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACACTCA CAGGAGCCAT GTCCTTGCTG
    CGGCGCTGCA GAGTCAATGC TGCCTTGACC ATCCAGCTCT TCTCTCAGCT TTTCCACTTC
    ATCAATATGT GGCTGTTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGCTCCCAC
    TACTGGGGTG CCATTATCCG CCAGCAGCTG GGGCATATTG AAGCGTGGGC AGAGAAGCAG
    GGGCTAGAGC TGGCAGCTGA CTGTCACTTG AGCAGAATTG TACAGGCTAC AACATTGCTT
    ACGATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA
    AATTCTCTGC AGCTTCAAGC CTTGTTACAA AATTACCACT GTGCACCCGA TGAGCCTTTT
    ATTCCTACGG ATCTCATAGA AAATGTTGTG GCTGTTGCTG AGAACACAGC TGATGAGCTC
    GCCCGCAGTG ATGGCAGAGA CGTGCAGCTG GAGGAAGACC CAGACCTACA GCTGCCTTTT
    CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA
    GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGTAGAT TGGTTCCTCA CACACGTTCA
    CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT TATGCGTGAG
    AACGCAGAAC TGGCCCAGCC TCTGAGGAAG GAGCCTGAGA TAATCACTGT GACTCTGAAG
    AAGCAGAATG GAATGGGCCT CAGCATCGTT GCAGCCAAGG GTGCTGGCCA GGATAAACTG
    GGAATCTACG TTAAGTCGGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGATTAGCT
    GCTGGTGACC AACTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG
    GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTTACAC TGGAAGTTGC AAAGCAAGGT
    GCCATCTATC ACGGCCTGGC CACCCTCCTT AACCAGCCGT CCCCCATGAT GCAAAGAATT
    TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGTG AAGGTTTTGA GCTCTATAAT
    AATTCAGCTC AAAATGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG
    CCAAAGAAAC TGCCTGGGGA TGACAGGCTG ATGAAAAACA GAGCGGACCA CCGTTCCAGC
    CCCAATGTGG CAAATCAGCC TCCTAGCCCT GGAGGGAAGG GTCCATATAC ATCTGGAACA
    GCAGCTAAGA TAACATCTGT CTCCACTGGA AACCTCTGCA CTGAGGAGCA GTCCCCTCCA
    CCCAGACCTG AAGCCTATCC TATCCCTACT CAGACATACA CCAGAGAGTA TTTTACCTTT
    CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTCCTCAGA GCCAGTGGCC AAATTATGAG
    GAAAAACCAC ATGTGCACAC AGAAAGTAAT CATTCCAGTA TTGCAATCCA GCGTGTTACC
    CGTTCCCAGG AAGAGCTTCG GGAAGAAAAG GTTTACCAAC TTGAGCGGCA TCGAGTAGAG
    GCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA
    TCCAGCACAT CCAGCCAGGA ACACCTGAAC CATTCTTCCA AGTCAGTTAC CCCTGCTTCC
    ACTCTGACCA AGAGTGGTCC TGGCCGTTGG AAAACACCAG CAGCGGTGCT GCCCACCCCT
    GTGGCTGTCT CTCAGCCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCGCCGGTC
    CACTACACCA GTGAGTTTGA TGGTATTCCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC
    AACCAGGCAG GACCCCAGTC TGCTCAGGTG GCTGCTGCAG AGTGGAAAAA GAGAGAGGAG
    CACCAGAGGT GGTATGAGAA AGAGAAGGCC CGCCTGGAGG AAGAACGAGA GAGAAAGCGC
    AGGGAGCAGG AGCGGAAGTT GGGGCAGATG CGCTCTCAGA CCCTGAACCC TGCTTCCTTC
    TCACCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA
    ACAGTCATTC GGGAGCTTCA GCCCCAGCAG CAGCCCCGCA CGATCGAGCG CAAGGATCTG
    CAGTATATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGTG ATAGTCTGTC TCCAGACCCC
    TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTGGACATG
    CTAAGCAAGG AGATCCACGA GCTGCAAAAC AAGGTGGACC GTACTGCGGA GGAGAGTGAC
    CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAACAG
    AAGGATGAGG ATGATGACGA AGAGGAAGAT GATGATGTGG ACACCATGCT GATCATGCAG
    CGTCTGGAGG CCGAGCGGAG AGCCAGGATG CAGGATGAGG AGCGCAGGCG CCAGCAACAG
    TTGGAAGAGA TGCGGAAACG AGAAGCAGAA GATCGAGTAA GACAAGAGGA AGATGGACGC
    CATCAGGAGG AGGAGCGAGT AAAGAGAGAT GCTGAAGAAA AGAGGCGACA GGAGGAAGGG
    TATTACAGCC GCCTAGAAGC TGAGAGGCGC CGACAGCACG AAGAGGCAGC ACGCAGGCTG
    CTGGAGCCTG AAGAGCCCGG GCTGAGCCGA CCTCCACTTC CACGGGACTA TGAGCCCCCG
    TCCTTGTCCT CAGCACCCTG TGCCCCTCCT CCCCCACCTC AGCGAAACGC ATCCTACCTC
    AAAACACAGG TCCTCTCCCC AGACTCGCTG TTCACTGCCA AGTTTGTTGC GTATGATGAG
    GAGGAGGAGG ACTACGGCCC AGCAGGACCA AACTCTTACT CGGGATCTGC AGGCACAGCT
    GTGGGAGCCT ATGATGCCCC TCGGGAAGCG AGAGAGAAAC TGACTAGGAG CCAAGACGCA
    GACTTACCTG GCAGTTCTGG AGCCCCTGAA AATCTGACAT TTAAAGAGCG TCAGCGCCTT
    TTTTCACAAG GGCAGGATGT GTCTGACAAA GTGAAAGCTT CTCGTAAATT AACAGAGCTC
    GAGAATGAAC TGAACACAAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53220
    Clone ID Related Accession (Same CDS sequence) XM_006523766.2 , XM_006523766.3
    Accession Version XM_006523766.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523829.1
    CDS131..5632
    Translation

    Target ORF information:

    RefSeq Version XM_006523766.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAGAAAGAA GATTGGACAT TGATGAGAAA CCTCTAGTTG TACAGTTGAA TTGGAACAAA
    GATGATCGAG AGGGTCGATT CGTCCTTAAG AACGAGAATG ACGCCATTCC TGCCAAGAAG
    GCTCAGAGTA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATTCAGAA CTTCAAAAGA
    ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAAAG AGAAGGAGGC ATTGAGACAA
    GCCTCTGATA AGGAAGAAAG GCCTTCTCAA GGGGATGACA GTGAGAACTC ACGACTGGCT
    GCTGAAGTTT ACAAAGACAT GCCTGAGACC AGCTTTACTA GGACCATTTC TAACCCTGAA
    GTTGTCATGA AACGGCGGCG GCAGCAAAAA CTGGAGAAGA GAATGCAAGA GTTTCGGAGC
    TCAGATGGGC GGCCTGACTC AGGTGGAACA CTGAGGATAT ATGCAGATAG TTTAAAACCA
    AATATTCCAT ACAAGACAAT CCTGCTGTCT ACTACCGATA CCGCAGACTT TGCTGTAGCC
    GAATCTTTGG AGAAGTATGG TCTGGAAAAA GAGAATCCCA AGGACTACTG TATTGCCCGG
    GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAGAGAG GTGCTAAGGA AATAATTCTT
    GACGATGATG AATGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA
    GTCTTTCAGT TGAAGAGGAG ACCACCGGAC TACATCCCAA AGAAAATGAA GAAACATGTT
    GAAGGGAAGT CGTTGAAGGG GAAAGACAGA GCTGACGGGT CTGGCTACGG CTCCGCTCTT
    CCTCCCGAGA AGCTGCCCTA CTTAGTAGAG CTAAGCCCAG GGAGAAGGAA TCACTTTGCC
    TACTACAGCT ATCACACTTA CGAAGATGGC TCTGACTCCA GAGACAAGCC AAAGTTATAC
    CGCCTTCAAT TAAGTGTGAC TGAAGTTGGG ACAGAGAAAT TTGATGACAA TTCTATCCAG
    CTGTTTGGCC CCGGAATTCA GCCCCATCAC TGTGATCTCA CCAACATGGA TGGAGTGGTC
    ACTGTGACAC CCAGAAGTAT GGATGCAGAG ACCTACGTGG ATGGTCAGCG CATCTCAGAG
    ACCACAATGC TGCAGAGTGG CATGAGACTG CAGTTTGGCA CCTCGCATGT GTTTAAGTTT
    GTGGACCCCA TCCAGGACCA CGTTCTTTCC AAGAGATCTG TGGATGGAGG CCTGATGGTC
    AAGGGCCCAA GACATAAACC TGGAGCTGTT CAGGAGACAA CCTTTGAATT GGGAGGAGAT
    GTCCACAGTG GGACAGCATT GCCGGCAAGC AGGAGCACTA CTAGACTGGA CAGTGACAGG
    GTGTCCTCTG CCTCTAGCAC AGCTGAGCGA GGGATGGTGA AGCCGATGAT CCGACTGGAC
    CAGGAGCAGG AATATCGACG ACGAGAAAAC AGGACTCAGG ATGCTACTGG GCCTGAACTG
    ATACTGCCTG CCAGCATTGA ATTCAGGGAA AGCTCTGAAG ACTCTTTTTT ATCTGCCATT
    ATAAACTATA CCAATAGTTC TACAGTTCAT TTTAAGTTGT CACCTACATA TGTGTTATAC
    ATGGCATGCC GGTATGTATT GTCCAGCCAG CACAGGCCCG ACATCAGTCC TACAGAGCGT
    ACACATAAGG CCATTGCTGT TGTCAACAAG ATGGTGAGCA TGATGGAAGG GGTCATCCAG
    AAACAGAAGA ACATTGCAGG AGCACTTGCT TTTTGGATGG CAAATGCATC TGAGCTTCTC
    AACTTCATCA AGCAGGACCG AGACCTTAGT CGGATCACGC TGGATGCCCA GGATGTCCTA
    GCACACTTGG TTCAGATGGC ATTTAAGTAC TTGGTTCATT GCCTTCAGTC AGAACTTAAT
    AACTACATGC CAGCCTTTCT GGATGATCCT GAGGAGAACA GTCTGCAAAG ACCAAAAATA
    GATGATGTGC TGCACACACT CACAGGAGCC ATGTCCTTGC TGCGGCGCTG CAGAGTCAAT
    GCTGCCTTGA CCATCCAGCT CTTCTCTCAG CTTTTCCACT TCATCAATAT GTGGCTGTTC
    AACAGACTGG TGACTGACCC AGATTCAGGC CTGTGCTCCC ACTACTGGGG TGCCATTATC
    CGCCAGCAGC TGGGGCATAT TGAAGCGTGG GCAGAGAAGC AGGGGCTAGA GCTGGCAGCT
    GACTGTCACT TGAGCAGAAT TGTACAGGCT ACAACATTGC TTACGATGGA TAAGTATGTA
    CCTGATGACA TTCCAAATAT AAACAGCACA TGCTTTAAGT TAAATTCTCT GCAGCTTCAA
    GCCTTGTTAC AAAATTACCA CTGTGCACCC GATGAGCCTT TTATTCCTAC GGATCTCATA
    GAAAATGTTG TGGCTGTTGC TGAGAACACA GCTGATGAGC TCGCCCGCAG TGATGGCAGA
    GACGTGCAGC TGGAGGAAGA CCCAGACCTA CAGCTGCCTT TTCTTCTGCC AGAAGATGGC
    TATTCCTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG
    TGCCAGAGAG GATTTTGTAG ATTGGTTCCT CACACACGTT CACCAGGTAC CTGGACAATA
    TATTTTGAAG GAGCAGATTA TGAAAGTCAC CTTATGCGTG AGAACGCAGA ACTGGCCCAG
    CCTCTGAGGA AGGAGCCTGA GATAATCACT GTGACTCTGA AGAAGCAGAA TGGAATGGGC
    CTCAGCATCG TTGCAGCCAA GGGTGCTGGC CAGGATAAAC TGGGAATCTA CGTTAAGTCG
    GTTGTCAAAG GAGGTGCTGC AGATGTGGAT GGACGATTAG CTGCTGGTGA CCAACTCCTC
    AGTGTGGATG GACGAAGTCT GGTTGGACTT TCTCAGGAAA GGGCAGCAGA ACTCATGACA
    AGAACCAGTT CCGTGGTTAC ACTGGAAGTT GCAAAGCAAG GTGCCATCTA TCACGGCCTG
    GCCACCCTCC TTAACCAGCC GTCCCCCATG ATGCAAAGAA TTTCAGATCG TCGTGGCTCA
    GGTAAACCCA GACCAAAGAG TGAAGGTTTT GAGCTCTATA ATAATTCAGC TCAAAATGGC
    TCCCCAGAGA GTCCTCAGAT GCCTTGGACA GAGTACAGTG AGCCAAAGAA ACTGCCTGGG
    GATGACAGGC TGATGAAAAA CAGAGCGGAC CACCGTTCCA GCCCCAATGT GGCAAATCAG
    CCTCCTAGCC CTGGAGGGAA GGGTCCATAT ACATCTGGAA CAGCAGCTAA GATAACATCT
    GTCTCCACTG GAAACCTCTG CACTGAGGAG CAGTCCCCTC CACCCAGACC TGAAGCCTAT
    CCTATCCCTA CTCAGACATA CACCAGAGAG TATTTTACCT TTCCAGCTTC AAAGTCGCAG
    GATCGGATGG CTCCTCCTCA GAGCCAGTGG CCAAATTATG AGGAAAAACC ACATGTGCAC
    ACAGAAAGTA ATCATTCCAG TATTGCAATC CAGCGTGTTA CCCGTTCCCA GGAAGAGCTT
    CGGGAAGAAA AGGTTTACCA ACTTGAGCGG CATCGAGTAG AGGCAGGCAT GGATCGCAAG
    TGTGACAGTG ATATGTGGAT CAATCAGAGC TCCTCAGTGG AATCCAGCAC ATCCAGCCAG
    GAACACCTGA ACCATTCTTC CAAGTCAGTT ACCCCTGCTT CCACTCTGAC CAAGAGTGGT
    CCTGGCCGTT GGAAAACACC AGCAGCGGTG CTGCCCACCC CTGTGGCTGT CTCTCAGCCC
    ATTCGAACAG ACCTCCCTCC ACCACCACCT CCACCGCCGG TCCACTACAC CAGTGAGTTT
    GATGGTATTC CAATGGATTT GCCCCTCCCA CCTCCTCCTG CCAACCAGGC AGGACCCCAG
    TCTGCTCAGG TGGCTGCTGC AGAGTGGAAA AAGAGAGAGG AGCACCAGAG GTGGTATGAG
    AAAGAGAAGG CCCGCCTGGA GGAAGAACGA GAGAGAAAGC GCAGGGAGCA GGAGCGGAAG
    TTGGGGCAGA TGCGCTCTCA GACCCTGAAC CCTGCTTCCT TCTCACCTTT GGCCACACAG
    GCCAAGCCAG AAAAGCCTTC CACACTGCAG AGGCCCCAAG AAACAGTCAT TCGGGAGCTT
    CAGCCCCAGC AGCAGCCCCG CACGATCGAG CGCAAGGATC TGCAGTATAT AACCATCAGT
    AAAGAGGAGC TGTCCTCTGG TGATAGTCTG TCTCCAGACC CCTGGAAACG AGATGCCAGG
    GAGAAGCTGG AGAAGCAACA GCAAATGCAC ATTGTGGACA TGCTAAGCAA GGAGATCCAC
    GAGCTGCAAA ACAAGGTGGA CCGTACTGCG GAGGAGAGTG ACCGCCTGCG GAAGCTTATG
    CTGGAGTGGC AGTTCCAGAA GAGACTACAG GAGTCCAAAC AGAAGGATGA GGATGATGAC
    GAAGAGGAAG ATGATGATGT GGACACCATG CTGATCATGC AGCGTCTGGA GGCCGAGCGG
    AGAGCCAGGC AGACTGCTAT GCCAGCAATC TCTGTTCTAG ATTTGATGCA GGATGAGGAG
    CGCAGGCGCC AGCAACAGTT GGAAGAGATG CGGAAACGAG AAGCAGAAGA TCGAGTAAGA
    CAAGAGGAAG ATGGACGCCA TCAGGAGGAG GAGCGAGTAA AGAGAGATGC TGAAGAAAAG
    AGGCGACAGG AGGAAGGGTA TTACAGCCGC CTAGAAGCTG AGAGGCGCCG ACAGCACGAA
    GAGGCAGCAC GCAGGCTGCT GGAGCCTGAA GAGCCCGGGC TGAGCCGACC TCCACTTCCA
    CGGGACTATG AGCCCCCGTC CTTGTCCTCA GCACCCTGTG CCCCTCCTCC CCCACCTCAG
    CGAAACGCAT CCTACCTCAA AACACAGGTC CTCTCCCCAG ACTCGCTGTT CACTGCCAAG
    TTTGTTGCGT ATGATGAGGA GGAGGAGGAC TACGGCCCAG CAGGACCAAA CTCTTACTCG
    GGATCTGCAG GCACAGCTGT GGGAGCCTAT GATGCCCCTC GGGAAGCGAG AGAGAAACTG
    ACTAGGAGCC AAGACGCAGA CTTACCTGGC AGTTCTGGAG CCCCTGAAAA TCTGACATTT
    AAAGAGCGTC AGCGCCTTTT TTCACAAGGG CAGGATGTGT CTGACAAAGT GAAAGCTTCT
    CGTAAATTAA CAGAGCTCGA GAATGAACTG AACACAAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53221
    Clone ID Related Accession (Same CDS sequence) XM_006523767.2 , XM_006523767.3
    Accession Version XM_006523767.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product afadin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGAT GCAGGATGAG GAGCGCAGGC GCCAGCAACA GTTGGAAGAG
    ATGCGGAAAC GAGAAGCAGA AGATCGAGTA AGACAAGAGG AAGATGGACG CCATCAGGAG
    GAGGAGCGAG TAAAGAGAGA TGCTGAAGAA AAGAGGCGAC AGGAGGAAGG GTATTACAGC
    CGCCTAGAAG CTGAGAGGCG CCGACAGCAC GAAGAGGCAG CACGCAGGCT GCTGGAGCCT
    GAAGAGCCCG GGCTGAGCCG ACCTCCACTT CCACGGGACT ATGAGCCCCC GTCCTTGTCC
    TCAGCACCCT GTGCCCCTCC TCCCCCACCT CAGCGAAACG CATCCTACCT CAAAACACAG
    GTCCTCTCCC CAGACTCGCT GTTCACTGCC AAGTTTGTTG CGTATGATGA GGAGGAGGAG
    GACTACGGCC CAGCAGGTCA GGATAAGTAC CCCAGCACAC GGAAGTCTCA TGGGCACCTT
    CCTCTTGCCA CCCTTAAGCC GCAGCAGCCC CCCTGCCAGC CACGCCCAGC CTCGGATGGC
    ATCTCCCTCT GCAACTCATT CCAGCCACCC TTGGCCAAAG CCAACAGTAC AGCTTACAAA
    GCAGGCCAGC CCCCGCCCCT GCCAGGCAAA CCAAGCCTGT ACCGTCCTGG CCACTGGAAA
    GGTCGAGGAC CAAACTCTTA CTCGGGATCT GCAGGCACAG CTGTGGGAGC CTATGATGCC
    CCTCGGGAAG CGAGAGAGAA ACTGACTAGG AGCCAAGACG CAGACTTACC TGGCAGTTCT
    GGAGCCCCTG AAAATCTGAC ATTTAAAGAG CGTCAGCGCC TTTTTTCACA AGGGCAGGAT
    GTGTCTGACA AAGTGAAAGC TTCTCGTAAA TTAACAGAGC TCGAGAATGA ACTGAACACA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523830.1
    CDS131..5776
    Translation

    Target ORF information:

    RefSeq Version XM_006523767.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (Mllt4), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006523767.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
    AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT
    GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT
    CGAGTCTCTA GCACAGCCAC CACTCAAGAT GTGATTGAAA CACTGGCAGA GAAGTTCCGC
    CCTGACATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA
    GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT
    GATCGAGAGG GTCGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGGCTCAG
    AGTAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATTC AGAACTTCAA AAGAACTCTC
    TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AAAGAGAAGG AGGCATTGAG ACAAGCCTCT
    GATAAGGAAG AAAGGCCTTC TCAAGGGGAT GACAGTGAGA ACTCACGACT GGCTGCTGAA
    GTTTACAAAG ACATGCCTGA GACCAGCTTT ACTAGGACCA TTTCTAACCC TGAAGTTGTC
    ATGAAACGGC GGCGGCAGCA AAAACTGGAG AAGAGAATGC AAGAGTTTCG GAGCTCAGAT
    GGGCGGCCTG ACTCAGGTGG AACACTGAGG ATATATGCAG ATAGTTTAAA ACCAAATATT
    CCATACAAGA CAATCCTGCT GTCTACTACC GATACCGCAG ACTTTGCTGT AGCCGAATCT
    TTGGAGAAGT ATGGTCTGGA AAAAGAGAAT CCCAAGGACT ACTGTATTGC CCGGGTTATG
    CTTCCTCCTG GAGCCCAGCA TTCTGATGAG AGAGGTGCTA AGGAAATAAT TCTTGACGAT
    GATGAATGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT
    CAGTTGAAGA GGAGACCACC GGACTACATC CCAAAGAAAA TGAAGAAACA TGTTGAAGGG
    AAGTCGTTGA AGGGGAAAGA CAGAGCTGAC GGGTCTGGCT ACGGCTCCGC TCTTCCTCCC
    GAGAAGCTGC CCTACTTAGT AGAGCTAAGC CCAGATGGCT CTGACTCCAG AGACAAGCCA
    AAGTTATACC GCCTTCAATT AAGTGTGACT GAAGTTGGGA CAGAGAAATT TGATGACAAT
    TCTATCCAGC TGTTTGGCCC CGGAATTCAG CCCCATCACT GTGATCTCAC CAACATGGAT
    GGAGTGGTCA CTGTGACACC CAGAAGTATG GATGCAGAGA CCTACGTGGA TGGTCAGCGC
    ATCTCAGAGA CCACAATGCT GCAGAGTGGC ATGAGACTGC AGTTTGGCAC CTCGCATGTG
    TTTAAGTTTG TGGACCCCAT CCAGGACCAC GTTCTTTCCA AGAGATCTGT GGATGGAGGC
    CTGATGGTCA AGGGCCCAAG ACATAAACCT GGAGCTGTTC AGGAGACAAC CTTTGAATTG
    GGAGGAGATG TCCACAGTGG GACAGCATTG CCGGCAAGCA GGAGCACTAC TAGACTGGAC
    AGTGACAGGG TGTCCTCTGC CTCTAGCACA GCTGAGCGAG GGATGGTGAA GCCGATGATC
    CGACTGGACC AGGAGCAGGA ATATCGACGA CGAGAAAACA GGACTCAGGA TGCTACTGGG
    CCTGAACTGA TACTGCCTGC CAGCATTGAA TTCAGGGAAA GCTCTGAAGA CTCTTTTTTA
    TCTGCCATTA TAAACTATAC CAATAGTTCT ACAGTTCATT TTAAGTTGTC ACCTACATAT
    GTGTTATACA TGGCATGCCG GTATGTATTG TCCAGCCAGC ACAGGCCCGA CATCAGTCCT
    ACAGAGCGTA CACATAAGGC CATTGCTGTT GTCAACAAGA TGGTGAGCAT GATGGAAGGG
    GTCATCCAGA AACAGAAGAA CATTGCAGGA GCACTTGCTT TTTGGATGGC AAATGCATCT
    GAGCTTCTCA ACTTCATCAA GCAGGACCGA GACCTTAGTC GGATCACGCT GGATGCCCAG
    GATGTCCTAG CACACTTGGT TCAGATGGCA TTTAAGTACT TGGTTCATTG CCTTCAGTCA
    GAACTTAATA ACTACATGCC AGCCTTTCTG GATGATCCTG AGGAGAACAG TCTGCAAAGA
    CCAAAAATAG ATGATGTGCT GCACACACTC ACAGGAGCCA TGTCCTTGCT GCGGCGCTGC
    AGAGTCAATG CTGCCTTGAC CATCCAGCTC TTCTCTCAGC TTTTCCACTT CATCAATATG
    TGGCTGTTCA ACAGACTGGT GACTGACCCA GATTCAGGCC TGTGCTCCCA CTACTGGGGT
    GCCATTATCC GCCAGCAGCT GGGGCATATT GAAGCGTGGG CAGAGAAGCA GGGGCTAGAG
    CTGGCAGCTG ACTGTCACTT GAGCAGAATT GTACAGGCTA CAACATTGCT TACGATGGAT
    AAGTATGTAC CTGATGACAT TCCAAATATA AACAGCACAT GCTTTAAGTT AAATTCTCTG
    CAGCTTCAAG CCTTGTTACA AAATTACCAC TGTGCACCCG ATGAGCCTTT TATTCCTACG
    GATCTCATAG AAAATGTTGT GGCTGTTGCT GAGAACACAG CTGATGAGCT CGCCCGCAGT
    GATGGCAGAG ACGTGCAGCT GGAGGAAGAC CCAGACCTAC AGCTGCCTTT TCTTCTGCCA
    GAAGATGGCT ATTCCTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA
    GACCCTCTGT GCCAGAGAGG ATTTTGTAGA TTGGTTCCTC ACACACGTTC ACCAGGTACC
    TGGACAATAT ATTTTGAAGG AGCAGATTAT GAAAGTCACC TTATGCGTGA GAACGCAGAA
    CTGGCCCAGC CTCTGAGGAA GGAGCCTGAG ATAATCACTG TGACTCTGAA GAAGCAGAAT
    GGAATGGGCC TCAGCATCGT TGCAGCCAAG GGTGCTGGCC AGGATAAACT GGGAATCTAC
    GTTAAGTCGG TTGTCAAAGG AGGTGCTGCA GATGTGGATG GACGATTAGC TGCTGGTGAC
    CAACTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTTT CTCAGGAAAG GGCAGCAGAA
    CTCATGACAA GAACCAGTTC CGTGGTTACA CTGGAAGTTG CAAAGCAAGG TGCCATCTAT
    CACGGCCTGG CCACCCTCCT TAACCAGCCG TCCCCCATGA TGCAAAGAAT TTCAGATCGT
    CGTGGCTCAG GTAAACCCAG ACCAAAGAGT GAAGGTTTTG AGCTCTATAA TAATTCAGCT
    CAAAATGGCT CCCCAGAGAG TCCTCAGATG CCTTGGACAG AGTACAGTGA GCCAAAGAAA
    CTGCCTGGGG ATGACAGGCT GATGAAAAAC AGAGCGGACC ACCGTTCCAG CCCCAATGTG
    GCAAATCAGC CTCCTAGCCC TGGAGGGAAG GGTCCATATA CATCTGGAAC AGCAGCTAAG
    ATAACATCTG TCTCCACTGG AAACCTCTGC ACTGAGGAGC AGTCCCCTCC ACCCAGACCT
    GAAGCCTATC CTATCCCTAC TCAGACATAC ACCAGAGAGT ATTTTACCTT TCCAGCTTCA
    AAGTCGCAGG ATCGGATGGC TCCTCCTCAG AGCCAGTGGC CAAATTATGA GGAAAAACCA
    CATGTGCACA CAGAAAGTAA TCATTCCAGT ATTGCAATCC AGCGTGTTAC CCGTTCCCAG
    GAAGAGCTTC GGGAAGAAAA GGTTTACCAA CTTGAGCGGC ATCGAGTAGA GGCAGGCATG
    GATCGCAAGT GTGACAGTGA TATGTGGATC AATCAGAGCT CCTCAGTGGA ATCCAGCACA
    TCCAGCCAGG AACACCTGAA CCATTCTTCC AAGTCAGTTA CCCCTGCTTC CACTCTGACC
    AAGAGTGGTC CTGGCCGTTG GAAAACACCA GCAGCGGTGC TGCCCACCCC TGTGGCTGTC
    TCTCAGCCCA TTCGAACAGA CCTCCCTCCA CCACCACCTC CACCGCCGGT CCACTACACC
    AGTGAGTTTG ATGGTATTCC AATGGATTTG CCCCTCCCAC CTCCTCCTGC CAACCAGGCA
    GGACCCCAGT CTGCTCAGGT GGCTGCTGCA GAGTGGAAAA AGAGAGAGGA GCACCAGAGG
    TGGTATGAGA AAGAGAAGGC CCGCCTGGAG GAAGAACGAG AGAGAAAGCG CAGGGAGCAG
    GAGCGGAAGT TGGGGCAGAT GCGCTCTCAG ACCCTGAACC CTGCTTCCTT CTCACCTTTG
    GCCACACAGG CCAAGCCAGA AAAGCCTTCC ACACTGCAGA GGCCCCAAGA AACAGTCATT
    CGGGAGCTTC AGCCCCAGCA GCAGCCCCGC ACGATCGAGC GCAAGGATCT GCAGTATATA
    ACCATCAGTA AAGAGGAGCT GTCCTCTGGT GATAGTCTGT CTCCAGACCC CTGGAAACGA
    GATGCCAGGG AGAAGCTGGA GAAGCAACAG CAAATGCACA TTGTGGACAT GCTAAGCAAG
    GAGATCCACG AGCTGCAAAA CAAGGTGGAC CGTACTGCGG AGGAGAGTGA CCGCCTGCGG
    AAGCTTATGC TGGAGTGGCA GTTCCAGAAG AGACTACAGG AGTCCAAACA GAAGGATGAG
    GATGATGACG AAGAGGAAGA TGATGATGTG GACACCATGC TGATCATGCA GCGTCTGGAG
    GCCGAGCGGA GAGCCAGGAT GCAGGATGAG GAGCGCAGGC GCCAGCAACA GTTGGAAGAG
    ATGCGGAAAC GAGAAGCAGA AGATCGAGTA AGACAAGAGG AAGATGGACG CCATCAGGAG
    GAGGAGCGAG TAAAGAGAGA TGCTGAAGAA AAGAGGCGAC AGGAGGAAGG GTATTACAGC
    CGCCTAGAAG CTGAGAGGCG CCGACAGCAC GAAGAGGCAG CACGCAGGCT GCTGGAGCCT
    GAAGAGCCCG GGCTGAGCCG ACCTCCACTT CCACGGGACT ATGAGCCCCC GTCCTTGTCC
    TCAGCACCCT GTGCCCCTCC TCCCCCACCT CAGCGAAACG CATCCTACCT CAAAACACAG
    GTCCTCTCCC CAGACTCGCT GTTCACTGCC AAGTTTGTTG CGTATGATGA GGAGGAGGAG
    GACTACGGCC CAGCAGGTCA GGATAAGTAC CCCAGCACAC GGAAGTCTCA TGGGCACCTT
    CCTCTTGCCA CCCTTAAGCC GCAGCAGCCC CCCTGCCAGC CACGCCCAGC CTCGGATGGC
    ATCTCCCTCT GCAACTCATT CCAGCCACCC TTGGCCAAAG CCAACAGTAC AGCTTACAAA
    GCAGGCCAGC CCCCGCCCCT GCCAGGCAAA CCAAGCCTGT ACCGTCCTGG CCACTGGAAA
    GGTCGAGGAC CAAACTCTTA CTCGGGATCT GCAGGCACAG CTGTGGGAGC CTATGATGCC
    CCTCGGGAAG CGAGAGAGAA ACTGACTAGG AGCCAAGACG CAGACTTACC TGGCAGTTCT
    GGAGCCCCTG AAAATCTGAC ATTTAAAGAG CGTCAGCGCC TTTTTTCACA AGGGCAGGAT
    GTGTCTGACA AAGTGAAAGC TTCTCGTAAA TTAACAGAGC TCGAGAATGA ACTGAACACA
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.