Mep1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mep1b
Entrez Gene ID	17288
Full Name	meprin 1 beta
Synonyms	Mep-1b
General protein information	Preferred Names meprin 1 beta Names meprin A subunit beta endopeptidase-2 meprin B meprin beta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 A2\|18 11.81 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008586.2	NP_032612.2	meprin A subunit beta precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MEP1B	NP_001137570.1
Gallus gallus (chicken)	MEP1B	XP_419181.3
Danio rerio (zebrafish)	si:ch73-40a17.3	XP_003197854.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012881	XP_306106.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010764	XP_318543.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011475	XP_317825.4
Homo sapiens (human)	MEP1B	NP_005916.2
	MEP1B	XP_001100668.1
Mus musculus (house mouse)	Mep1b	NP_032612.2
Drosophila melanogaster (fruit fly)	CG6696	NP_573318.1
Xenopus tropicalis (tropical clawed frog)	mep1b	NP_001096352.1
Canis lupus familiaris (dog)	MEP1B	XP_005623043.1
Rattus norvegicus (Norway rat)	Mep1b	NP_037315.1
Pan troglodytes (chimpanzee)	MEP1B	XP_523904.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010758	XP_318553.4

Gene Ontology
Bibliography

Related articles in PubMed

Mucus Detachment by Host Metalloprotease Meprin ? Requires Shedding of Its Inactive Pro-form, which Is Abrogated by the Pathogenic Protease RgpB.
Wichert R, Ermund A, Schmidt S, Schweinlin M, Ksiazek M, Arnold P, Knittler K, Wilkens F, Potempa B, Rabe B, Stirnberg M, Lucius R, Bartsch JW, Nikolaus S, Falk-Paulsen M, Rosenstiel P, Metzger M, Rose-John S, Potempa J, Hansson GC, Dempsey PJ, Becker-Pauly C
Cell reports21(8)2090-2103(2017 Nov)

Deficiency of the DSPP-cleaving enzymes meprin ? and meprin ? does not result in dentin malformation in mice.
Arnold P, Koopmann L, Peters F, Birkenfeld F, Goff SV, Damm T, Qin C, Moali C, Lucius R, Becker-Pauly C
Cell and tissue research367(2)351-358(2017 Feb)

Meprin ? contributes to collagen deposition in lung fibrosis.
Biasin V, Wygrecka M, Marsh LM, Becker-Pauly C, Brcic L, Ghanim B, Klepetko W, Olschewski A, Kwapiszewska G
Scientific reports739969(2017 Jan)

Meprin Metalloprotease Deficiency Associated with Higher Mortality Rates and More Severe Diabetic Kidney Injury in Mice with STZ-Induced Type 1 Diabetes.
Bylander JE, Ahmed F, Conley SM, Mwiza JM, Ongeri EM
Journal of diabetes research20179035038(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

no significant dentin malformation was observed in Mep1b (-/-) or Mep1a (-/-) deficient mice.
Title: Deficiency of the DSPP-cleaving enzymes meprin ? and meprin ? does not result in dentin malformation in mice.

meprin alpha and meprin beta join the modulators of Reelin signalling as they cleave Reelin at a specific site and are upregulated under specific pathological conditions.
Title: Determination of cleavage site of Reelin between its sixth and seventh repeat and contribution of meprin metalloproteases to the cleavage.

These studies provide strong evidence for a pathophysiological link between meprin beta and urinary excretion of cleaved nidogen-1 during cisplatin-induced acute kidney injury.
Title: Basement membrane protein nidogen-1 is a target of meprin ? in cisplatin nephrotoxicity.

While meprin A only cleaved protein kinase A (PKA) catalytic subunit beta1, meprin B cleaved all three PKA catalytic isoforms.
Title: Isoform-specific interactions between meprin metalloproteases and the catalytic subunit of protein kinase A: significance in acute and chronic kidney injury.

Meprin beta is an endogenous zinc-dependent metalloprotease now shown to cleave the N-terminal region of the MUC2 mucin at two specific sites.
Title: Microbial-induced meprin ? cleavage in MUC2 mucin and a functional CFTR channel are required to release anchored small intestinal mucus.

The following Mep1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mep1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02281	NM_008586.2 Latest version!	Mus musculus meprin 1 beta (Mep1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$419.30 $599.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mep1b ( NM_008586.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032612.2 Mus musculus meprin 1 beta (Mep1b), mRNA.

Price & Availability↑

CloneID	OMu02281
Clone ID Related Accession (Same CDS sequence)	NM_008586.2
Accession Version	NM_008586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	meprin A subunit beta precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK098127.1. On May 22, 2007 this sequence version replaced NM_008586.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK098127.1, BC145979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END##

ATGGATGCCC GGCATCAGCC TTGGTTTCTG GTTTTTGCCA CATTTCTCCT GGTTTCTGGT 
TTGCCAGCTC CGGAAAAGTT TGTCAAAGAC ATAGATGGAG GAATTGACCA AGACATATTT 
GATATTAACC AAGGTTTGGG TCTGGACCTT TTTGAGGGAG ACATCAAACT CGAGGCAAAT 
GGGAAGAATT CCATCATTGG AGACCACAAG AGATGGCCAC ATACCATTCC ATATGTTCTA 
GAGGACAGCT TGGAAATGAA TGCTAAAGGA GTTATCCTCA ATGCTTTTGA GCGCTATCGC 
CTTAAAACAT GCATTGACTT CAAGCCTTGG TCCGGAGAAG CTAACTATAT CTCAGTGTTC 
AAGGGCAGTG GGTGCTGGTC TTCAGTGGGA AACATTCATG CTGGGAAGCA GGAGTTGTCC 
ATTGGGACAA ACTGTGACAG AATAGCAACA GTTCAGCATG AGTTCCTCCA CGCCCTGGGA 
TTCTGGCATG AGCAGTCACG TGCTGACCGG GATGATTACG TCATTATAGT TTGGGACAGG 
ATTCAGCCAG GCAAGGAACA CAACTTCAAC ATCTACAATG ACAGTGTGTC TGATTCCCTG 
AATGTCCCAT ATGACTACAC CTCAGTAATG CACTACAGTA AAACCGCTTT CCAGAACGGG 
ACAGAGTCTA CCATCGTCAC GAGAATCTCA GAGTTCGAGG ACGTGATTGG CCAACGAATG 
GACTTCAGTG ACTATGACCT TCTGAAGCTA AATCAGCTGT ATAACTGCAC TTCTTCCTTG 
AGTTTTATGG ACTCCTGTGA TTTTGAATTG GAAAATATCT GTGGCATGAT CCAAAGTTCG 
GGGGATAGTG CTGACTGGCA GCGGGTTTCA CAGGTTCTCA GTGGCCCAGA GAGCGACCAC 
TCCAAGATGG GCCAGTGCAA AGACTCTGGC TTCTTCATGC ATTTCAACAC TAGCATTTTG 
AATGAGGGGG CCACGGCGAT GTTGGAGAGC AGACTGTTGT ACCCCAAGAG AGGGTTTCAG 
TGCTTGGAGT TTTATCTGTA CAACAGTGGA AGTGGAAATG ACCAACTGAA CATTTACACC 
CGGGAGTACA CTACAGGCCA GCAGGGTGGT GTTTTAACCC TTCAGAGACA AATAAAAGAG 
GTACCCATTG GGAGCTGGCA ACTTCACTAT GTAACACTGC AAGTGACTAA AAAGTTTCGA 
GTGGTGTTTG AAGGACTCAG AGGCCCTGGC ACGTCATCAG GTGGTCTGTC TATCGATGAC 
ATCAATCTCT CAGAAACAAG GTGTCCTCAC CATATCTGGC ACATACAGAA TTTCACACAG 
ATTCTAGGCG GCCAGGACAC ATCTGTATAC AGTCCTCCAT TTTATTCTTC TAAAGGTTAC 
GCTTTTCAGA TCTACATGGA TCTAAGATCC TCAACAAATG TAGGAATTTA TTTCCACCTG 
ATCTCCGGTG CCAATGATGA TCAATTACAG TGGCCATGTC CTTGGCAACA AGCTACAATG 
ACACTCTTGG ATCAAAATCC TGACATCCGA CAGCGTATGT TCAACCAGCG GAGTATAACC 
ACAGACCCAA CGATGACCAG TGATAATGGA AGCTACTTTT GGGACAGGCC TTCCAAGGTG 
GGAGTGACGG ATGTTTTCCC TAACGGAACT CAGTTTAGCA GAGGTATAGG CTATGGAACG 
ACTGTCTTCA TAACCCGAGA GAGGCTGAAG AGCAGAGAGT TCATAAAAGG AGATGACATT 
TACATCTTAC TGACTGTTGA AGACATATCT CACCTCAATT CTACATCAGC TGTCCCCGAC 
CCAGTCCCCA CCTTGGCTGT CCATAATGCC TGCTCTGAGG TTGTATGTCA GAACGGTGGC 
ATCTGTGTTG TCCAAGATGG CAGAGCTGAG TGCAAGTGTC CTGCAGGAGA AGACTGGTGG 
TACATGGGCA AAAGGTGTGA GAAGAGAGGG TCCACCCGAG ACACTGTTAT CATCGCTGTT 
TCTTCCACGG TCACCGTGTT TGCTGTGATG CTAATCATCA CTCTCGTCAG TGTCTACTGC 
ACCAGGAGGA AATATCGTAA GAAGGCTAGA GCAAATACGG CAGCCATGAC TCTAGAAAAC 
CAACATGCGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032612.2
CDS	31..2145
Translation	MDARHQPWFLVFATFLLVSGLPAPEKFVKDIDGGIDQDIFDINQGLGLDLFEGDIKLEANGKNSIIGDHKRWPHTIPYVLEDSLEMNAKGVILNAFERYRLKTCIDFKPWSGEANYISVFKGSGCWSSVGNIHAGKQELSIGTNCDRIATVQHEFLHALGFWHEQSRADRDDYVIIVWDRIQPGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAFQNGTESTIVTRISEFEDVIGQRMDFSDYDLLKLNQLYNCTSSLSFMDSCDFELENICGMIQSSGDSADWQRVSQVLSGPESDHSKMGQCKDSGFFMHFNTSILNEGATAMLESRLLYPKRGFQCLEFYLYNSGSGNDQLNIYTREYTTGQQGGVLTLQRQIKEVPIGSWQLHYVTLQVTKKFRVVFEGLRGPGTSSGGLSIDDINLSETRCPHHIWHIQNFTQILGGQDTSVYSPPFYSSKGYAFQIYMDLRSSTNVGIYFHLISGANDDQLQWPCPWQQATMTLLDQNPDIRQRMFNQRSITTDPTMTSDNGSYFWDRPSKVGVTDVFPNGTQFSRGIGYGTTVFITRERLKSREFIKGDDIYILLTVEDISHLNSTSAVPDPVPTLAVHNACSEVVCQNGGICVVQDGRAECKCPAGEDWWYMGKRCEKRGSTRDTVIIAVSSTVTVFAVMLIITLVSVYCTRRKYRKKARANTAAMTLENQHAF

Target ORF information:

RefSeq Version	NM_008586.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus meprin 1 beta (Mep1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008586.2

ORF Insert Sequence:

ATGGATGCCC GGCATCAGCC TTGGTTTCTG GTTTTTGCCA CATTTCTCCT GGTTTCTGGT 
TTGCCAGCTC CGGAAAAGTT TGTCAAAGAC ATAGATGGAG GAATTGACCA AGACATATTT 
GATATTAACC AAGGTTTGGG TCTGGACCTT TTTGAGGGAG ACATCAAACT CGAGGCAAAT 
GGGAAGAATT CCATCATTGG AGACCACAAG AGATGGCCAC ATACCATTCC ATATGTTCTA 
GAGGACAGCT TGGAAATGAA TGCTAAAGGA GTTATCCTCA ATGCTTTTGA GCGCTATCGC 
CTTAAAACAT GCATTGACTT CAAGCCTTGG TCCGGAGAAG CTAACTATAT CTCAGTGTTC 
AAGGGCAGTG GGTGCTGGTC TTCAGTGGGA AACATTCATG CTGGGAAGCA GGAGTTGTCC 
ATTGGGACAA ACTGTGACAG AATAGCAACA GTTCAGCATG AGTTCCTCCA CGCCCTGGGA 
TTCTGGCATG AGCAGTCACG TGCTGACCGG GATGATTACG TCATTATAGT TTGGGACAGG 
ATTCAGCCAG GCAAGGAACA CAACTTCAAC ATCTACAATG ACAGTGTGTC TGATTCCCTG 
AATGTCCCAT ATGACTACAC CTCAGTAATG CACTACAGTA AAACCGCTTT CCAGAACGGG 
ACAGAGTCTA CCATCGTCAC GAGAATCTCA GAGTTCGAGG ACGTGATTGG CCAACGAATG 
GACTTCAGTG ACTATGACCT TCTGAAGCTA AATCAGCTGT ATAACTGCAC TTCTTCCTTG 
AGTTTTATGG ACTCCTGTGA TTTTGAATTG GAAAATATCT GTGGCATGAT CCAAAGTTCG 
GGGGATAGTG CTGACTGGCA GCGGGTTTCA CAGGTTCTCA GTGGCCCAGA GAGCGACCAC 
TCCAAGATGG GCCAGTGCAA AGACTCTGGC TTCTTCATGC ATTTCAACAC TAGCATTTTG 
AATGAGGGGG CCACGGCGAT GTTGGAGAGC AGACTGTTGT ACCCCAAGAG AGGGTTTCAG 
TGCTTGGAGT TTTATCTGTA CAACAGTGGA AGTGGAAATG ACCAACTGAA CATTTACACC 
CGGGAGTACA CTACAGGCCA GCAGGGTGGT GTTTTAACCC TTCAGAGACA AATAAAAGAG 
GTACCCATTG GGAGCTGGCA ACTTCACTAT GTAACACTGC AAGTGACTAA AAAGTTTCGA 
GTGGTGTTTG AAGGACTCAG AGGCCCTGGC ACGTCATCAG GTGGTCTGTC TATCGATGAC 
ATCAATCTCT CAGAAACAAG GTGTCCTCAC CATATCTGGC ACATACAGAA TTTCACACAG 
ATTCTAGGCG GCCAGGACAC ATCTGTATAC AGTCCTCCAT TTTATTCTTC TAAAGGTTAC 
GCTTTTCAGA TCTACATGGA TCTAAGATCC TCAACAAATG TAGGAATTTA TTTCCACCTG 
ATCTCCGGTG CCAATGATGA TCAATTACAG TGGCCATGTC CTTGGCAACA AGCTACAATG 
ACACTCTTGG ATCAAAATCC TGACATCCGA CAGCGTATGT TCAACCAGCG GAGTATAACC 
ACAGACCCAA CGATGACCAG TGATAATGGA AGCTACTTTT GGGACAGGCC TTCCAAGGTG 
GGAGTGACGG ATGTTTTCCC TAACGGAACT CAGTTTAGCA GAGGTATAGG CTATGGAACG 
ACTGTCTTCA TAACCCGAGA GAGGCTGAAG AGCAGAGAGT TCATAAAAGG AGATGACATT 
TACATCTTAC TGACTGTTGA AGACATATCT CACCTCAATT CTACATCAGC TGTCCCCGAC 
CCAGTCCCCA CCTTGGCTGT CCATAATGCC TGCTCTGAGG TTGTATGTCA GAACGGTGGC 
ATCTGTGTTG TCCAAGATGG CAGAGCTGAG TGCAAGTGTC CTGCAGGAGA AGACTGGTGG 
TACATGGGCA AAAGGTGTGA GAAGAGAGGG TCCACCCGAG ACACTGTTAT CATCGCTGTT 
TCTTCCACGG TCACCGTGTT TGCTGTGATG CTAATCATCA CTCTCGTCAG TGTCTACTGC 
ACCAGGAGGA AATATCGTAA GAAGGCTAGA GCAAATACGG CAGCCATGAC TCTAGAAAAC 
CAACATGCGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mucus Detachment by Host Metalloprotease Meprin ? Requires Shedding of Its Inactive Pro-form, which Is Abrogated by the Pathogenic Protease RgpB.
Cell reports21(8)2090-2103(2017 Nov)
Wichert R,Ermund A,Schmidt S,Schweinlin M,Ksiazek M,Arnold P,Knittler K,Wilkens F,Potempa B,Rabe B,Stirnberg M,Lucius R,Bartsch JW,Nikolaus S,Falk-Paulsen M,Rosenstiel P,Metzger M,Rose-John S,Potempa J,Hansson GC,Dempsey PJ,Becker-Pauly C

Deficiency of the DSPP-cleaving enzymes meprin ? and meprin ? does not result in dentin malformation in mice.
Cell and tissue research367(2)351-358(2017 Feb)
Arnold P,Koopmann L,Peters F,Birkenfeld F,Goff SV,Damm T,Qin C,Moali C,Lucius R,Becker-Pauly C

Meprin ? contributes to collagen deposition in lung fibrosis.
Scientific reports739969(2017 Jan)
Biasin V,Wygrecka M,Marsh LM,Becker-Pauly C,Brcic L,Ghanim B,Klepetko W,Olschewski A,Kwapiszewska G

Meprin Metalloprotease Deficiency Associated with Higher Mortality Rates and More Severe Diabetic Kidney Injury in Mice with STZ-Induced Type 1 Diabetes.
Journal of diabetes research20179035038(2017)
Bylander JE,Ahmed F,Conley SM,Mwiza JM,Ongeri EM

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Mep1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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