Mep1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mep1a
Entrez Gene ID	17287
Full Name	meprin 1 alpha
Synonyms	AI098089,AW107200,Mep-1,Mep-1a,Mep1
General protein information	Preferred Names meprin 1 alpha Names meprin A subunit alpha endopeptidase-2 meprin A alpha-subunit meprin alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B3\|17 19.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008585.2	NP_032611.2	meprin A subunit alpha
XM_006523751.4	XP_006523814.1	meprin A subunit alpha isoform X1
XM_006523751.3	XP_006523814.1	meprin A subunit alpha isoform X1
NM_008585.3	NP_032611.2	meprin A subunit alpha

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MEP1A	XP_003587735.2
Mus musculus (house mouse)	Mep1a	NP_032611.2
Xenopus tropicalis (tropical clawed frog)	mep1a	NP_001015767.1
Gallus gallus (chicken)	MEP1A	NP_001264650.1
	MEP1A	XP_001103444.1
Danio rerio (zebrafish)	mep1a.2	NP_001122199.1
Canis lupus familiaris (dog)	MEP1A	XP_538947.3
Rattus norvegicus (Norway rat)	Mep1a	NP_037275.1
Homo sapiens (human)	MEP1A	NP_005579.2
Pan troglodytes (chimpanzee)	MEP1A	XP_518520.3

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency of the DSPP-cleaving enzymes meprin ? and meprin ? does not result in dentin malformation in mice.
Arnold P, Koopmann L, Peters F, Birkenfeld F, Goff SV, Damm T, Qin C, Moali C, Lucius R, Becker-Pauly C
Cell and tissue research367(2)351-358(2017 Feb)

Meprin ? contributes to collagen deposition in lung fibrosis.
Biasin V, Wygrecka M, Marsh LM, Becker-Pauly C, Brcic L, Ghanim B, Klepetko W, Olschewski A, Kwapiszewska G
Scientific reports739969(2017 Jan)

The anti-inflammatory peptide Ac-SDKP is released from thymosin-?4 by renal meprin-? and prolyl oligopeptidase.
Kumar N, Nakagawa P, Janic B, Romero CA, Worou ME, Monu SR, Peterson EL, Shaw J, Valeriote F, Ongeri EM, Niyitegeka JM, Rhaleb NE, Carretero OA
American journal of physiology. Renal physiology310(10)F1026-34(2016 May)

Determination of cleavage site of Reelin between its sixth and seventh repeat and contribution of meprin metalloproteases to the cleavage.
Sato Y, Kobayashi D, Kohno T, Kidani Y, Prox J, Becker-Pauly C, Hattori M
Journal of biochemistry159(3)305-12(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

no significant dentin malformation was observed in Mep1b (-/-) or Mep1a (-/-) deficient mice.
Title: Deficiency of the DSPP-cleaving enzymes meprin ? and meprin ? does not result in dentin malformation in mice.

meprin alpha and meprin beta join the modulators of Reelin signalling as they cleave Reelin at a specific site and are upregulated under specific pathological conditions.
Title: Determination of cleavage site of Reelin between its sixth and seventh repeat and contribution of meprin metalloproteases to the cleavage.

While meprin A only cleaved protein kinase A (PKA) catalytic subunit beta1, meprin B cleaved all three PKA catalytic isoforms.
Title: Isoform-specific interactions between meprin metalloproteases and the catalytic subunit of protein kinase A: significance in acute and chronic kidney injury.

meprin alpha and meprin beta are unique in their ability to process and release both C- and N-propeptides from type I procollagen in vitro and in vivo
Title: Metalloproteases meprin ? and meprin ? are C- and N-procollagen proteinases important for collagen assembly and tensile strength.

the binding of S-MBP to meprins triggers the complement activation through the lectin pathway and may cause the acute renal failure due to ischemia/reperfusion injury on kidney transplantation and hemorrhagic shock
Title: Role of interaction of mannan-binding protein with meprins at the initial step of complement activation in ischemia/reperfusion injury to mouse kidney.

The following Mep1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mep1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22315	NM_008585.3 Latest version!	Mus musculus meprin 1 alpha (Mep1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22315	XM_006523751.4 Latest version!	Mus musculus meprin 1 alpha (Mep1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu22315	XM_006523751.3	Mus musculus meprin 1 alpha (Mep1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22315	NM_008585.2	Mus musculus meprin 1 alpha (Mep1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mep1a ( NM_008585.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032611.2 Mus musculus meprin 1 alpha (Mep1a), mRNA.

Price & Availability↑

CloneID	OMu22315
Clone ID Related Accession (Same CDS sequence)	NM_008585.2 , XM_006523751.4 , XM_006523751.3 , NM_008585.3
Accession Version	NM_008585.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-20
Organism	Mus musculus(house mouse)
Product	meprin A subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC015258.1. On Jun 19, 2003 this sequence version replaced NM_008585.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015258.1, AK093914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032611.2
CDS	17..2299
Translation	MARRLGRSSSFAIMLWIQPACLLSLIFSAHIAAVSIKHLLNGSDHDTDVGEQKDIFEINLAAGLNLFQGDILLPRTRNAMRDPSSRWKLPIPYILADNLELNAKGAILHAFEMFRLKSCVDFKPYEGESSYIIFQKLSGCWSMIGDQQVGQNISIGEGCDFKATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSFNKNESIPTITTKIPEFNTIIGQLPDFSAIDLIRLNRMYNCTATHTLLDHCDFEKTNVCGMIQGTRDDADWAHGDSSQPEQVDHTLVGQCKGAGYFMFFNTSLGARGEAALLESRILYPKRKQQCLQFFYKMTGSPADRFEVWVRRDDNAGKVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFLGTKGDPGNSSGGIYLDDITLTETPCPAGVWTIRNISQILENTVKGDKLVSPRFYNSEGYGVGVTLYPNGRITSNSGFLGLTFHLYSGDNDAILEWPVENRQAIMTILDQEADTRNRMSLTLMFTTSKNQTSSAINGSVIWDRPSKVGVYDKDCDCFRSLDWGWGQAISHQLLKRRNFLKGDSLIIFVDFKDLTHLNRTEVPASARSTMPRGLLLQGQESPALGESSRKAMLEESLPSSLGQRHPSRQKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYAGERCQAMHVHGSLLGLLIGCIAGLIFLTFVTFSTTNGKLRQ

Target ORF information:

RefSeq Version	NM_008585.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus meprin 1 alpha (Mep1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008585.2

ORF Insert Sequence:

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mep1a ( NM_008585.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032611.2 Mus musculus meprin 1 alpha (Mep1a), mRNA.

Price & Availability↑

CloneID	OMu22315
Clone ID Related Accession (Same CDS sequence)	NM_008585.2 , XM_006523751.4 , XM_006523751.3 , NM_008585.3
Accession Version	NM_008585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	meprin A subunit alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT010585.12. On Jan 22, 2019 this sequence version replaced NM_008585.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015258.1, AK143906.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032611.2
CDS	17..2299
Translation	MARRLGRSSSFAIMLWIQPACLLSLIFSAHIAAVSIKHLLNGSDHDTDVGEQKDIFEINLAAGLNLFQGDILLPRTRNAMRDPSSRWKLPIPYILADNLELNAKGAILHAFEMFRLKSCVDFKPYEGESSYIIFQKLSGCWSMIGDQQVGQNISIGEGCDFKATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSFNKNESIPTITTKIPEFNTIIGQLPDFSAIDLIRLNRMYNCTATHTLLDHCDFEKTNVCGMIQGTRDDADWAHGDSSQPEQVDHTLVGQCKGAGYFMFFNTSLGARGEAALLESRILYPKRKQQCLQFFYKMTGSPADRFEVWVRRDDNAGKVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFLGTKGDPGNSSGGIYLDDITLTETPCPAGVWTIRNISQILENTVKGDKLVSPRFYNSEGYGVGVTLYPNGRITSNSGFLGLTFHLYSGDNDAILEWPVENRQAIMTILDQEADTRNRMSLTLMFTTSKNQTSSAINGSVIWDRPSKVGVYDKDCDCFRSLDWGWGQAISHQLLKRRNFLKGDSLIIFVDFKDLTHLNRTEVPASARSTMPRGLLLQGQESPALGESSRKAMLEESLPSSLGQRHPSRQKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYAGERCQAMHVHGSLLGLLIGCIAGLIFLTFVTFSTTNGKLRQ

Target ORF information:

RefSeq Version	NM_008585.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus meprin 1 alpha (Mep1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008585.3

ORF Insert Sequence:

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mep1a ( XM_006523751.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523814.1 Mus musculus meprin 1 alpha (Mep1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22315
Clone ID Related Accession (Same CDS sequence)	NM_008585.2 , XM_006523751.4 , XM_006523751.3 , NM_008585.3
Accession Version	XM_006523751.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	meprin A subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523751.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523814.1
CDS	142..2424
Translation	MARRLGRSSSFAIMLWIQPACLLSLIFSAHIAAVSIKHLLNGSDHDTDVGEQKDIFEINLAAGLNLFQGDILLPRTRNAMRDPSSRWKLPIPYILADNLELNAKGAILHAFEMFRLKSCVDFKPYEGESSYIIFQKLSGCWSMIGDQQVGQNISIGEGCDFKATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSFNKNESIPTITTKIPEFNTIIGQLPDFSAIDLIRLNRMYNCTATHTLLDHCDFEKTNVCGMIQGTRDDADWAHGDSSQPEQVDHTLVGQCKGAGYFMFFNTSLGARGEAALLESRILYPKRKQQCLQFFYKMTGSPADRFEVWVRRDDNAGKVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFLGTKGDPGNSSGGIYLDDITLTETPCPAGVWTIRNISQILENTVKGDKLVSPRFYNSEGYGVGVTLYPNGRITSNSGFLGLTFHLYSGDNDAILEWPVENRQAIMTILDQEADTRNRMSLTLMFTTSKNQTSSAINGSVIWDRPSKVGVYDKDCDCFRSLDWGWGQAISHQLLKRRNFLKGDSLIIFVDFKDLTHLNRTEVPASARSTMPRGLLLQGQESPALGESSRKAMLEESLPSSLGQRHPSRQKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYAGERCQAMHVHGSLLGLLIGCIAGLIFLTFVTFSTTNGKLRQ

Target ORF information:

RefSeq Version	XM_006523751.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus meprin 1 alpha (Mep1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523751.3

ORF Insert Sequence:

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mep1a ( XM_006523751.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523814.1 Mus musculus meprin 1 alpha (Mep1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22315
Clone ID Related Accession (Same CDS sequence)	NM_008585.2 , XM_006523751.4 , XM_006523751.3 , NM_008585.3
Accession Version	XM_006523751.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	meprin A subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523751.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523814.1
CDS	144..2426
Translation	MARRLGRSSSFAIMLWIQPACLLSLIFSAHIAAVSIKHLLNGSDHDTDVGEQKDIFEINLAAGLNLFQGDILLPRTRNAMRDPSSRWKLPIPYILADNLELNAKGAILHAFEMFRLKSCVDFKPYEGESSYIIFQKLSGCWSMIGDQQVGQNISIGEGCDFKATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSFNKNESIPTITTKIPEFNTIIGQLPDFSAIDLIRLNRMYNCTATHTLLDHCDFEKTNVCGMIQGTRDDADWAHGDSSQPEQVDHTLVGQCKGAGYFMFFNTSLGARGEAALLESRILYPKRKQQCLQFFYKMTGSPADRFEVWVRRDDNAGKVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFLGTKGDPGNSSGGIYLDDITLTETPCPAGVWTIRNISQILENTVKGDKLVSPRFYNSEGYGVGVTLYPNGRITSNSGFLGLTFHLYSGDNDAILEWPVENRQAIMTILDQEADTRNRMSLTLMFTTSKNQTSSAINGSVIWDRPSKVGVYDKDCDCFRSLDWGWGQAISHQLLKRRNFLKGDSLIIFVDFKDLTHLNRTEVPASARSTMPRGLLLQGQESPALGESSRKAMLEESLPSSLGQRHPSRQKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYAGERCQAMHVHGSLLGLLIGCIAGLIFLTFVTFSTTNGKLRQ

Target ORF information:

RefSeq Version	XM_006523751.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus meprin 1 alpha (Mep1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523751.4

ORF Insert Sequence:

ATGGCAAGGA GGCTTGGCAG ATCCAGTTCA TTTGCAATAA TGCTTTGGAT ACAACCTGCG 
TGTCTTCTCT CACTGATTTT TTCTGCCCAC ATAGCAGCTG TATCGATTAA GCATCTTTTG 
AATGGATCTG ATCACGATAC AGATGTTGGT GAACAGAAGG ATATTTTCGA AATCAATTTA 
GCTGCAGGCC TGAACCTCTT TCAAGGGGAC ATCCTTCTGC CCAGAACCAG AAATGCCATG 
CGAGATCCCT CAAGCAGATG GAAGCTCCCC ATTCCATACA TTCTGGCTGA CAATCTGGAG 
CTGAATGCCA AAGGAGCCAT CCTCCATGCC TTTGAGATGT TCCGCCTCAA GTCTTGTGTG 
GATTTCAAGC CCTACGAAGG GGAGAGTTCG TATATCATCT TCCAGAAACT TTCTGGGTGC 
TGGTCTATGA TTGGTGATCA ACAGGTGGGA CAGAACATTT CCATTGGTGA GGGATGTGAC 
TTTAAGGCCA CCATTGAACA TGAAATCCTG CATGCTCTGG GATTCTTCCA TGAGCAGTCA 
AGGACTGACC GGGATGATTA TGTGAACATT TGGTGGGATC AAATCATTAC AGACTATGAA 
CATAATTTCA ACACATATGA CGACAACACC ATCACAGACC TCAACACACC CTATGATTAT 
GAGTCCCTGA TGCACTACGG GCCATTTTCG TTTAACAAGA ACGAAAGCAT CCCTACCATT 
ACTACCAAGA TCCCTGAGTT TAACACCATC ATTGGACAAC TCCCGGACTT CAGTGCCATT 
GATCTAATAA GACTGAATCG AATGTACAAC TGCACCGCAA CACATACTCT GTTGGACCAC 
TGTGACTTTG AGAAGACCAA CGTCTGTGGG ATGATCCAGG GAACCAGAGA TGATGCTGAT 
TGGGCTCATG GGGACAGCAG TCAGCCTGAA CAAGTGGATC ACACCTTGGT GGGACAGTGT 
AAAGGTGCCG GCTACTTCAT GTTCTTCAAT ACCAGCTTGG GGGCAAGAGG AGAGGCAGCC 
CTTCTGGAGT CTCGAATCCT TTATCCCAAG AGGAAGCAGC AGTGTTTGCA GTTTTTTTAT 
AAGATGACTG GAAGCCCTGC AGACAGATTT GAAGTCTGGG TGAGAAGGGA CGACAACGCG 
GGCAAGGTGC GACAGCTGGC CAAGATCCAG ACCTTTCAAG GAGACTCTGA TCACAACTGG 
AAAATTGCTC ATGTGACACT CAATGAAGAA AAGAAATTTC GCTATGTTTT CCTGGGTACC 
AAAGGGGACC CTGGTAACTC AAGTGGTGGT ATTTACCTTG ATGACATCAC TCTGACAGAA 
ACCCCCTGCC CTGCAGGGGT TTGGACCATC CGGAATATCT CCCAGATCCT TGAGAACACA 
GTGAAAGGGG ACAAGCTCGT GAGCCCTCGG TTCTACAATT CTGAGGGTTA CGGTGTGGGG 
GTGACCTTGT ACCCCAATGG TAGAATCACC AGTAACTCTG GCTTCCTGGG ACTCACTTTT 
CACTTGTACA GTGGGGATAA CGATGCGATT CTGGAGTGGC CAGTGGAAAA CAGACAGGCC 
ATAATGACCA TCCTGGACCA GGAAGCTGAT ACCAGGAACA GGATGTCCCT GACCTTGATG 
TTCACTACCT CCAAGAACCA AACATCTTCA GCTATAAATG GCTCTGTCAT CTGGGACAGG 
CCATCCAAGG TGGGAGTCTA TGATAAGGAC TGTGATTGTT TCAGAAGCCT TGACTGGGGC 
TGGGGTCAGG CCATCTCCCA CCAGCTGCTG AAGAGAAGAA ATTTTCTTAA AGGCGACAGC 
CTCATCATTT TCGTGGACTT TAAAGACCTC ACCCACCTGA ACCGGACTGA AGTTCCAGCT 
TCAGCCAGAA GCACGATGCC CAGAGGCCTC CTTCTGCAAG GCCAGGAGTC TCCTGCCTTG 
GGAGAAAGCT CCAGAAAAGC CATGCTGGAG GAATCCCTGC CAAGCAGCCT GGGCCAGAGA 
CATCCCAGCA GACAGAAGCG CTCGGTGGAG AACACTGGCC CCATGGAGGA CCACAACTGG 
CCACAGTACT TCAGAGACCC CTGTGACCCA AACCCTTGCC AGAATGAAGG CACCTGTGTG 
AACGTAAAGG GAATGGCCAG CTGCAGGTGC GTCTCTGGGC ACGCCTTTTT CTACGCAGGG 
GAGCGCTGTC AGGCCATGCA CGTGCACGGC AGCTTGCTGG GCCTGCTCAT TGGCTGCATC 
GCTGGCTTGA TCTTCTTGAC CTTCGTCACA TTCTCTACCA CCAACGGAAA GCTGCGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficiency of the DSPP-cleaving enzymes meprin ? and meprin ? does not result in dentin malformation in mice.
Cell and tissue research367(2)351-358(2017 Feb)
Arnold P,Koopmann L,Peters F,Birkenfeld F,Goff SV,Damm T,Qin C,Moali C,Lucius R,Becker-Pauly C

Meprin ? contributes to collagen deposition in lung fibrosis.
Scientific reports739969(2017 Jan)
Biasin V,Wygrecka M,Marsh LM,Becker-Pauly C,Brcic L,Ghanim B,Klepetko W,Olschewski A,Kwapiszewska G

The anti-inflammatory peptide Ac-SDKP is released from thymosin-?4 by renal meprin-? and prolyl oligopeptidase.
American journal of physiology. Renal physiology310(10)F1026-34(2016 May)
Kumar N,Nakagawa P,Janic B,Romero CA,Worou ME,Monu SR,Peterson EL,Shaw J,Valeriote F,Ongeri EM,Niyitegeka JM,Rhaleb NE,Carretero OA

Determination of cleavage site of Reelin between its sixth and seventh repeat and contribution of meprin metalloproteases to the cleavage.
Journal of biochemistry159(3)305-12(2016 Mar)
Sato Y,Kobayashi D,Kohno T,Kidani Y,Prox J,Becker-Pauly C,Hattori M

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Mep1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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