Anapc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Anapc1
Entrez Gene ID	17222
Full Name	anaphase promoting complex subunit 1
Synonyms	2610021O03Rik,AI047775,AI853536,AW547281,Apc1,Mcpr,tsg24
General protein information	Preferred Names anaphase promoting complex subunit 1 Names anaphase-promoting complex subunit 1 cyclosome subunit 1 meiotic check point regulator mitotic checkpoint regulator testis-specific gene 24 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030248086.1	XP_030103946.1	anaphase-promoting complex subunit 1 isoform X1
XM_011239333.3	XP_011237635.1	anaphase-promoting complex subunit 1 isoform X3
XM_011239332.3	XP_011237634.1	anaphase-promoting complex subunit 1 isoform X2
NM_008569.2	NP_032595.2	anaphase-promoting complex subunit 1
XM_011239332.2	XP_011237634.1	anaphase-promoting complex subunit 1 isoform X1
XM_011239333.2	XP_011237635.1	anaphase-promoting complex subunit 1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANAPC1	XP_005630492.1
Rattus norvegicus (Norway rat)	Anapc1	NP_001101241.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000374	XP_310737.5
Arabidopsis thaliana (thale cress)	EMB2771	NP_196175.1
Oryza sativa (rice)	Os05g0354300	NP_001055285.1
Drosophila melanogaster (fruit fly)	shtd	NP_573025.2
Xenopus tropicalis (tropical clawed frog)	anapc1	NP_001072866.1
Homo sapiens (human)	ANAPC1	NP_073153.1
Mus musculus (house mouse)	Anapc1	NP_032595.2
Danio rerio (zebrafish)	anapc1	XP_001921168.2
Bos taurus (cattle)	ANAPC1	XP_005212426.1
Gallus gallus (chicken)	ANAPC1	XP_419303.3

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Steinmetz CC, Tatavarty V, Sugino K, Shima Y, Joseph A, Lin H, Rutlin M, Lambo M, Hempel CM, Okaty BW, Paradis S, Nelson SB, Turrigiano GG
Cell reports16(10)2711-2722(2016 09)

The E3 ligase APC/C(Cdh1) promotes ubiquitylation-mediated proteolysis of PAX3 to suppress melanocyte proliferation and melanoma growth.
Cao J, Dai X, Wan L, Wang H, Zhang J, Goff PS, Sviderskaya EV, Xuan Z, Xu Z, Xu X, Hinds P, Flaherty KT, Faller DV, Goding CR, Wang Y, Wei W, Cui R
Science signaling8(392)ra87(2015 Sep)

The phosphatase-transcription activator EYA1 is targeted by anaphase-promoting complex/Cdh1 for degradation at M-to-G1 transition.
Sun J, Karoulia Z, Wong EY, Ahmed M, Itoh K, Xu PX
Molecular and cellular biology33(5)927-36(2013 Mar)

Tbx5-hedgehog molecular networks are essential in the second heart field for atrial septation.
Xie L, Hoffmann AD, Burnicka-Turek O, Friedland-Little JM, Zhang K, Moskowitz IP
Developmental cell23(2)280-91(2012 Aug)

Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK.
Fan M, Rhee J, St-Pierre J, Handschin C, Puigserver P, Lin J, J?eger S, Erdjument-Bromage H, Tempst P, Spiegelman BM
Genes & development18(3)278-89(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggest a tumor suppressor role for APC/C(Cdh1) in melanocytes and that targeting PAX3 may be a strategy for treating melanoma.
Title: The E3 ligase APC/C(Cdh1) promotes ubiquitylation-mediated proteolysis of PAX3 to suppress melanocyte proliferation and melanoma growth.

EYA1 is efficiently degraded during mitotic exit in a ANAPC1-dependent manner and these two proteins physically interact.
Title: The phosphatase-transcription activator EYA1 is targeted by anaphase-promoting complex/Cdh1 for degradation at M-to-G1 transition.

The following Anapc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Anapc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68190	XM_011239332.3 Latest version!	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu68191	XM_011239333.3 Latest version!	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87737	XM_030248086.1 Latest version!	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu13168	NM_008569.2 Latest version!	Mus musculus anaphase promoting complex subunit 1 (Anapc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1427.30 $2039.00
View Old Accession Versions >>
OMu68190	XM_011239332.2	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu68191	XM_011239333.2	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Anapc1 ( XM_011239332.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237634.1 Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68190
Clone ID Related Accession (Same CDS sequence)	XM_011239332.3 , XM_011239332.2
Accession Version	XM_011239332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	anaphase-promoting complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239332.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACT TCTCTGAAGA AAGGGCAACG ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTTG GCCGAGACCA CTGCAAGCAC CACCCTAACG CTCTGAACCT TCAGCTTCGT 
CAGCTGCAGC CAGCCTCTGA GTTATGGTCT TCTGATGGTG CAGCTGGCTT GGTGGGATCT 
CTTCAGGAGG TTACAATCCA TGAGAAACAG AAGGAAAGCT GGCAGTTAAG AAAAGGTGTG 
AGTGAGATTG GAGATGCAGC TGACTACGAT GAAGAGCTCT ATGTGGCTGG GAACATGGTT 
ATCTGGAGCA AAGGGAGTAA AAGCCAGGCA CTGGCAGTGT ACAAAGCGTT CACAGTGGAC 
AGCACTGTCC AGCAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAG 
ATCTATAAGA GCCATGAACT AGAGAAATGC ATATGTATAT TACAGAGTTC ATGTATGAAC 
ATGCATAGCA TAGACGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTT 
TGGGCCACTA AATATGGCTT GCTGTTTGAA CGATGCAGTT CTTCACATGA GGTACCTCCA 
AGTCTACCTA GAGAACCTTT GCCTACTATG TTCAGCATGC TGCACCCACT GGATGAGATA 
ACTCCACTTG TTTGTAAATC CGGAAGTCTT TTTGGCTCAT CACGGGTACA GTATGTTGTA 
GATCCAGCAG TGAAAATTGT GTTTCTGAAC ATTGACCCCT CCATTGTCAT GACCTATGAT 
GCTGTTCAGA ATGTGCATTC TGTGTGGACT CTGCGGAGGG TAAAGCCAGA GGAAGAGAAT 
GCTGTTTTAA AGTTCCCTGA GCAGGCAGGG ACCCTGCAGA ATGCAACTAC TAGCAGCTCC 
CTGACAGCAC ATCTCAGAAG CCTCTCCAAA GGCGAATCCC CTGTGGCTTC GCCGTTCCAG 
AATTACTCTT CCATTCACAG CCAGAGTCGC TCAACCTCTT CGCCCAGTCT ACACTCTCGG 
TCGCCTTCCA TTTCCAACAT GGCGGCTCTA AGTCGTGCTC ACTCTCCTGC CTTAGGTGTG 
CACTCTTTCT CAGGGGCGCA AAGATTCAAC CTTTCAAGCC ACAGTCAGTC ACCAAAGAGA 
CACAGCATTT CTCACTCTCC GAGTGGCAGT TTCAACGACT CATTTTTGGC ACCAGAAACA 
GAGCCAATTG TTCCTGAGCT GTGCATTGAT CACTTATGGA CTGAGACACT TCCGAATATA 
AGAGAGAAAA ATTCTCAAGC CTCCAAGGTA TTTATAACGA CTGACCTGTG TGGACAGAAG 
TTCTTGTGCT TTTTAGTGGA GGCCCAGCTT CAGTTACGCT GTGTAAAGTT TCAAGAGAGT 
AATGACAAGA CTCAGCTTAT CTTTGGCTCT GTCACCAACA TACATGCAAA AGATGCAGCC 
CCAGTGGAGA AGATACACAC CATGCTGGTC CTAGAAGGCA ACGGGAACCT GGTGCTGTAC 
ACAGGAGTGG TGCGGGTGGG GAAAGTCTTT ATCCCTGGAC TTCCGGCTCC TTCCCTGACA 
ATGTCCAACA TGATGCCTCG GCCCAGTACC CCGCTCGACG GTGTTGGTAC TCCAAAGCCT 
CTGAGTAAAT TGCTTGGATC GATGGATGAG GTGGTTCTGT TGTCTCCAGT TCCAGAATTG 
AGGGATTCTT CAAAACTTAA TGATTCTCTC TACAATGAGG ATTGCACATT CCAGCAGCTT 
GGAACTTACA TTCATTCTGT GCGAGACCCT GTTCATAACA GAGTCACTCT AGAACTGAGC 
AATGGCTCCA TGGTTCGGAT CACTATCCCT GAAGTGGCCA CCTCGGAATT AGTACAAACG 
TGTCTGCAAG CAATTAAGTT CATCCTGCCA AAAGAAGTAG CCATTCAGGT GCTCGTCAAG 
TGGTACAATG TGCACAGTGC TCCAGGAGGA CCCAGCTGTC ACTCAGAGTG GAGTTTATTT 
GTGATTTGTC TCTTGAACAT GATGGGTTAT AACACAGACC GCTTAGCATG GACCCGAAGT 
TTTGACTTTG AAGGATCACT TTCCCCAGTC ATTGCACCCA AAAAAGCAAG GCCTTCTGAT 
ACTGGATCTG ACGAGGACTG GGAGTACTTA CTGAACTCAG AGTACCATCG CAATGTTGAG 
TCTCATCTTT TGAACAAATC TCTATGTTTG ACTGCTTTGG AAGTTTCAAA TGCAAAGGAT 
GAAGATTTTT CGCAGAACCT CAGTCTGGAT TCTTCTACCC TTCTCTTTGC TCACATTCCT 
GCAATTTTCT TTGTTCTTCA CCTGGTTTAT GAAGAGCTCA AGTTGAATAC TCTAATGGGA 
GAAGGAATTT GTTCTCTTAT CGACCTCCTT GTTCAGTTGG CAAGGGACTT AAAATTGGAC 
TCTTACTTGG ACCATTACTA TCGAGATTCC CCAACTCTTG TCAAAACCAC TGGACAAGTA 
TGCACCATTG ACCAAGGTCA GATGGGATTT ATGCATCATC CCCCATTTTT TACTTCTGAG 
CCACCAAGTA TTTATCAGTG GGTGAGTTCA TGCCTGAAGG GTGAAGGGAT GCCACCCTAT 
CCTTACCTTC CTGGGATTTG CGAGAGGAGC AGGCTAGTGG TCTTGAGCAT TGCCCTCTAT 
ACCCTTGGTG ATGAGAGCTG TGTGTCTGAT GAAACTTGCC AGTACTTATC CAAAGTAACC 
TCAACTCCCC AAAAGCCACA AGCAGAGCAA GAAGAAAACA GGTTTACTTT CCGGCATTCT 
GCTTCCGTTT CTGTTCTGGC CGAGAGATTA GTTGTCTGGA TGGCCAGTGT AGGATTCACT 
TTAAGAGATC TGGAGACTCT TCCCTTTGGG ATTGCTCTTC CCATCAGAGA TGCGATTTAC 
CACTGTCGGG AGCAGCCTGA TTCAGATTGG TCAGAAGCGG TCTGTCTCTT GATTGGACGT 
CAGGATCTTT CCAAGCAGGC TTGTGAAGGA AATTTACCCA GAGGCAAATC TGTGCTCTCA 
TCAGAGGTGT CTTCAGGAAC TGAGGCAGAG GAGGAAGATG ATGGCATGAA TGACTTGAAC 
CATGAGGTTA TGTCATTAAT ATGGAGTGAA GATTTACGGG TGCAGGATGT GCGAAGGCTG 
CTTCAAAGTG CACAGCCTGT CCGTGTCAAT GTGGTGCAGT ACCCAGAACT CAGTGACCAT 
GAGTTCATTG AAGAAAAGGA AAACAGACTG CTCCAGTTGT GTCAGCGAAC TATGGCCCTT 
CCAGTAGGAC GAGGAATGTT TACCTTGTTC TCATATCATC CTGTTCCAAC AGAGCCGTTG 
CCTGTTCCTA AATTGAATCT GACAGGGCGA GCCCCTCCAC GAAACACAAC TGTAGATCTT 
AATAGCGGAA ACATCGACGT GCCTCCCAAC ATGGCCAGCT GGGCCAGCTT TCATAATGGT 
GTGGCTGCCG GCCTGAAGAT AGCCCCAGCC TCCCAGATAG ACTCAGCTTG GATCGTTTAC 
AACAAGCCAA AGCATGCTGA GTTAGCCAAT GAGTATGCCG GCTTTCTCAT GGCCCTGGGT 
CTGAATGGGC ACCTTACCAA GCTGGCTACT CTCAATATCC ATGACTATTT GACCAAGGGC 
CATGAAATGA CAAGCATTGG ATTGCTACTT GGTGTTTCTG CTGCAAAGCT TGGCACCATG 
GACATGTCAA TTACCCGGCT CCTCAGCATT CACGTTCCTG CTCTCTTACC CCCGACATCC 
ACAGAGCTTG ACGTGCCTCA CAATGTCCAA GTGGCGGCTG TGGTTGGCAT TGGCCTTGTG 
TATCAGGGCA CAGCTCACAG ACACACTGCG GAAGTCCTGT TGGCTGAAAT AGGTTCTTTT 
ACCTCGAACT CTTCTCTACC GATATCCCCT CCAACCCTAC CCACAACACT AGGTAGGAGA 
GAAAGAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237634.1
CDS	279..4190
Translation	MSNFSEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGDAADYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSTVQQALWCDFIISQDKSEKIYKSHELEKCICILQSSCMNMHSIDGKDYIASLPFQVANVWATKYGLLFERCSSSHEVPPSLPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKPEEENAVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFNDSFLAPETEPIVPELCIDHLWTETLPNIREKNSQASKVFITTDLCGQKFLCFLVEAQLQLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLVLEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQAIKFILPKEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSFDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHRNVESHLLNKSLCLTALEVSNAKDEDFSQNLSLDSSTLLFAHIPAIFFVLHLVYEELKLNTLMGEGICSLIDLLVQLARDLKLDSYLDHYYRDSPTLVKTTGQVCTIDQGQMGFMHHPPFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYTLGDESCVSDETCQYLSKVTSTPQKPQAEQEENRFTFRHSASVSVLAERLVVWMASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGSFTSNSSLPISPPTLPTTLGRRERR

Target ORF information:

RefSeq Version	XM_011239332.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239332.3

ORF Insert Sequence:

ATGTCGAACT TCTCTGAAGA AAGGGCAACG ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTTG GCCGAGACCA CTGCAAGCAC CACCCTAACG CTCTGAACCT TCAGCTTCGT 
CAGCTGCAGC CAGCCTCTGA GTTATGGTCT TCTGATGGTG CAGCTGGCTT GGTGGGATCT 
CTTCAGGAGG TTACAATCCA TGAGAAACAG AAGGAAAGCT GGCAGTTAAG AAAAGGTGTG 
AGTGAGATTG GAGATGCAGC TGACTACGAT GAAGAGCTCT ATGTGGCTGG GAACATGGTT 
ATCTGGAGCA AAGGGAGTAA AAGCCAGGCA CTGGCAGTGT ACAAAGCGTT CACAGTGGAC 
AGCACTGTCC AGCAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAG 
ATCTATAAGA GCCATGAACT AGAGAAATGC ATATGTATAT TACAGAGTTC ATGTATGAAC 
ATGCATAGCA TAGACGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTT 
TGGGCCACTA AATATGGCTT GCTGTTTGAA CGATGCAGTT CTTCACATGA GGTACCTCCA 
AGTCTACCTA GAGAACCTTT GCCTACTATG TTCAGCATGC TGCACCCACT GGATGAGATA 
ACTCCACTTG TTTGTAAATC CGGAAGTCTT TTTGGCTCAT CACGGGTACA GTATGTTGTA 
GATCCAGCAG TGAAAATTGT GTTTCTGAAC ATTGACCCCT CCATTGTCAT GACCTATGAT 
GCTGTTCAGA ATGTGCATTC TGTGTGGACT CTGCGGAGGG TAAAGCCAGA GGAAGAGAAT 
GCTGTTTTAA AGTTCCCTGA GCAGGCAGGG ACCCTGCAGA ATGCAACTAC TAGCAGCTCC 
CTGACAGCAC ATCTCAGAAG CCTCTCCAAA GGCGAATCCC CTGTGGCTTC GCCGTTCCAG 
AATTACTCTT CCATTCACAG CCAGAGTCGC TCAACCTCTT CGCCCAGTCT ACACTCTCGG 
TCGCCTTCCA TTTCCAACAT GGCGGCTCTA AGTCGTGCTC ACTCTCCTGC CTTAGGTGTG 
CACTCTTTCT CAGGGGCGCA AAGATTCAAC CTTTCAAGCC ACAGTCAGTC ACCAAAGAGA 
CACAGCATTT CTCACTCTCC GAGTGGCAGT TTCAACGACT CATTTTTGGC ACCAGAAACA 
GAGCCAATTG TTCCTGAGCT GTGCATTGAT CACTTATGGA CTGAGACACT TCCGAATATA 
AGAGAGAAAA ATTCTCAAGC CTCCAAGGTA TTTATAACGA CTGACCTGTG TGGACAGAAG 
TTCTTGTGCT TTTTAGTGGA GGCCCAGCTT CAGTTACGCT GTGTAAAGTT TCAAGAGAGT 
AATGACAAGA CTCAGCTTAT CTTTGGCTCT GTCACCAACA TACATGCAAA AGATGCAGCC 
CCAGTGGAGA AGATACACAC CATGCTGGTC CTAGAAGGCA ACGGGAACCT GGTGCTGTAC 
ACAGGAGTGG TGCGGGTGGG GAAAGTCTTT ATCCCTGGAC TTCCGGCTCC TTCCCTGACA 
ATGTCCAACA TGATGCCTCG GCCCAGTACC CCGCTCGACG GTGTTGGTAC TCCAAAGCCT 
CTGAGTAAAT TGCTTGGATC GATGGATGAG GTGGTTCTGT TGTCTCCAGT TCCAGAATTG 
AGGGATTCTT CAAAACTTAA TGATTCTCTC TACAATGAGG ATTGCACATT CCAGCAGCTT 
GGAACTTACA TTCATTCTGT GCGAGACCCT GTTCATAACA GAGTCACTCT AGAACTGAGC 
AATGGCTCCA TGGTTCGGAT CACTATCCCT GAAGTGGCCA CCTCGGAATT AGTACAAACG 
TGTCTGCAAG CAATTAAGTT CATCCTGCCA AAAGAAGTAG CCATTCAGGT GCTCGTCAAG 
TGGTACAATG TGCACAGTGC TCCAGGAGGA CCCAGCTGTC ACTCAGAGTG GAGTTTATTT 
GTGATTTGTC TCTTGAACAT GATGGGTTAT AACACAGACC GCTTAGCATG GACCCGAAGT 
TTTGACTTTG AAGGATCACT TTCCCCAGTC ATTGCACCCA AAAAAGCAAG GCCTTCTGAT 
ACTGGATCTG ACGAGGACTG GGAGTACTTA CTGAACTCAG AGTACCATCG CAATGTTGAG 
TCTCATCTTT TGAACAAATC TCTATGTTTG ACTGCTTTGG AAGTTTCAAA TGCAAAGGAT 
GAAGATTTTT CGCAGAACCT CAGTCTGGAT TCTTCTACCC TTCTCTTTGC TCACATTCCT 
GCAATTTTCT TTGTTCTTCA CCTGGTTTAT GAAGAGCTCA AGTTGAATAC TCTAATGGGA 
GAAGGAATTT GTTCTCTTAT CGACCTCCTT GTTCAGTTGG CAAGGGACTT AAAATTGGAC 
TCTTACTTGG ACCATTACTA TCGAGATTCC CCAACTCTTG TCAAAACCAC TGGACAAGTA 
TGCACCATTG ACCAAGGTCA GATGGGATTT ATGCATCATC CCCCATTTTT TACTTCTGAG 
CCACCAAGTA TTTATCAGTG GGTGAGTTCA TGCCTGAAGG GTGAAGGGAT GCCACCCTAT 
CCTTACCTTC CTGGGATTTG CGAGAGGAGC AGGCTAGTGG TCTTGAGCAT TGCCCTCTAT 
ACCCTTGGTG ATGAGAGCTG TGTGTCTGAT GAAACTTGCC AGTACTTATC CAAAGTAACC 
TCAACTCCCC AAAAGCCACA AGCAGAGCAA GAAGAAAACA GGTTTACTTT CCGGCATTCT 
GCTTCCGTTT CTGTTCTGGC CGAGAGATTA GTTGTCTGGA TGGCCAGTGT AGGATTCACT 
TTAAGAGATC TGGAGACTCT TCCCTTTGGG ATTGCTCTTC CCATCAGAGA TGCGATTTAC 
CACTGTCGGG AGCAGCCTGA TTCAGATTGG TCAGAAGCGG TCTGTCTCTT GATTGGACGT 
CAGGATCTTT CCAAGCAGGC TTGTGAAGGA AATTTACCCA GAGGCAAATC TGTGCTCTCA 
TCAGAGGTGT CTTCAGGAAC TGAGGCAGAG GAGGAAGATG ATGGCATGAA TGACTTGAAC 
CATGAGGTTA TGTCATTAAT ATGGAGTGAA GATTTACGGG TGCAGGATGT GCGAAGGCTG 
CTTCAAAGTG CACAGCCTGT CCGTGTCAAT GTGGTGCAGT ACCCAGAACT CAGTGACCAT 
GAGTTCATTG AAGAAAAGGA AAACAGACTG CTCCAGTTGT GTCAGCGAAC TATGGCCCTT 
CCAGTAGGAC GAGGAATGTT TACCTTGTTC TCATATCATC CTGTTCCAAC AGAGCCGTTG 
CCTGTTCCTA AATTGAATCT GACAGGGCGA GCCCCTCCAC GAAACACAAC TGTAGATCTT 
AATAGCGGAA ACATCGACGT GCCTCCCAAC ATGGCCAGCT GGGCCAGCTT TCATAATGGT 
GTGGCTGCCG GCCTGAAGAT AGCCCCAGCC TCCCAGATAG ACTCAGCTTG GATCGTTTAC 
AACAAGCCAA AGCATGCTGA GTTAGCCAAT GAGTATGCCG GCTTTCTCAT GGCCCTGGGT 
CTGAATGGGC ACCTTACCAA GCTGGCTACT CTCAATATCC ATGACTATTT GACCAAGGGC 
CATGAAATGA CAAGCATTGG ATTGCTACTT GGTGTTTCTG CTGCAAAGCT TGGCACCATG 
GACATGTCAA TTACCCGGCT CCTCAGCATT CACGTTCCTG CTCTCTTACC CCCGACATCC 
ACAGAGCTTG ACGTGCCTCA CAATGTCCAA GTGGCGGCTG TGGTTGGCAT TGGCCTTGTG 
TATCAGGGCA CAGCTCACAG ACACACTGCG GAAGTCCTGT TGGCTGAAAT AGGTTCTTTT 
ACCTCGAACT CTTCTCTACC GATATCCCCT CCAACCCTAC CCACAACACT AGGTAGGAGA 
GAAAGAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Anapc1 ( XM_011239333.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237635.1 Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu68191
Clone ID Related Accession (Same CDS sequence)	XM_011239333.3 , XM_011239333.2
Accession Version	XM_011239333.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	anaphase-promoting complex subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTG TAGGATTCAC TTTAAGAGAT CTGGAGACTC TTCCCTTTGG GATTGCTCTT 
CCCATCAGAG ATGCGATTTA CCACTGTCGG GAGCAGCCTG ATTCAGATTG GTCAGAAGCG 
GTCTGTCTCT TGATTGGACG TCAGGATCTT TCCAAGCAGG CTTGTGAAGG AAATTTACCC 
AGAGGCAAAT CTGTGCTCTC ATCAGAGGTG TCTTCAGGAA CTGAGGCAGA GGAGGAAGAT 
GATGGCATGA ATGACTTGAA CCATGAGGTT ATGTCATTAA TATGGAGTGA AGATTTACGG 
GTGCAGGATG TGCGAAGGCT GCTTCAAAGT GCACAGCCTG TCCGTGTCAA TGTGGTGCAG 
TACCCAGAAC TCAGTGACCA TGAGTTCATT GAAGAAAAGG AAAACAGACT GCTCCAGTTG 
TGTCAGCGAA CTATGGCCCT TCCAGTAGGA CGAGGAATGT TTACCTTGTT CTCATATCAT 
CCTGTTCCAA CAGAGCCGTT GCCTGTTCCT AAATTGAATC TGACAGGGCG AGCCCCTCCA 
CGAAACACAA CTGTAGATCT TAATAGCGGA AACATCGACG TGCCTCCCAA CATGGCCAGC 
TGGGCCAGCT TTCATAATGG TGTGGCTGCC GGCCTGAAGA TAGCCCCAGC CTCCCAGATA 
GACTCAGCTT GGATCGTTTA CAACAAGCCA AAGCATGCTG AGTTAGCCAA TGAGTATGCC 
GGCTTTCTCA TGGCCCTGGG TCTGAATGGG CACCTTACCA AGCTGGCTAC TCTCAATATC 
CATGACTATT TGACCAAGGG CCATGAAATG ACAAGCATTG GATTGCTACT TGGTGTTTCT 
GCTGCAAAGC TTGGCACCAT GGACATGTCA ATTACCCGGC TCCTCAGCAT TCACGTTCCT 
GCTCTCTTAC CCCCGACATC CACAGAGCTT GACGTGCCTC ACAATGTCCA AGTGGCGGCT 
GTGGTTGGCA TTGGCCTTGT GTATCAGGGC ACAGCTCACA GACACACTGC GGAAGTCCTG 
TTGGCTGAAA TAGGGCGGCC CCCTGGTCCA GAAATGGAAT ACTGCACTGA CAGAGAGTCG 
TACTCCTTAG CTGCTGGCCT GGCCCTGGGC ATGGTTTGCT TGGGGCATGG CAGCAATTTG 
ATTGGCATGT CTGATCTCAA TGTGCCTGAG CAGCTGTATC AGTACATGGT TGGAGGCCAT 
AGGCGATTTC AAACTGGAAT GCACAGGGAG AAACATAAGT CTCCAAGTTA TCAGATCAAA 
GAAGGAGACA CCATAAACGT GGATGTGACT TGTCCCGGTG CTACTCTGGC GTTGGCTATG 
ATCTACTTAA AAACCAATAA CAGGTCCATT GCTGATTGGC TGCGTGCTCC TGATACCATG 
TATTTGCTAG ACTTTGTGAA ACCAGAATTC CTTTTGCTTA GGACACTTGC TCGGTGCTTG 
ATTTTGTGGG ATGATATCTT ACCAAATTCC AAGTGGGTTG ACAGCAATGT TCCTCAGATT 
ATAAGAGAAA ATAGTATCTC TCTGAGTGAA ATTGAATTGC CTTGTTCAGA GGACTTGAAT 
TTGGAAACCT TGTCGCAAGC ACACGTCTAC ATCATTGCAG GAGCCTGCTT GTCTCTAGGG 
TTTCGATTTG CTGGCTCAGA AAACTTATCA GCATTTAGCT GTCTGCATAA ATTTGCAAAA 
GATTTTATGA ATTATTTATC TGCACCCAAT GCTTCTGTAA CAGGGCCCTA TAACCTCGAA 
ACCTGCCTGA GTGTGGTCCT GCTGTCTCTT GCCATGGTGA TGGCTGGCTC TGGGAACCTG 
AAGGTGTTGC AGCTCTGTCG CTTTCTGCAC ATGAAGACTG GTGGAGAGAT GAACTATGGC 
TTCCACTTGG CCCACCACAT GGCCCTGGGC CTTCTCTTTT TGGGAGGAGG AAGGTACTCT 
TTGAGCACAT CCAACTCATC CATTGCTGCC CTTCTCTGCG CCCTTTACCC ACATTTCCCA 
GCCCACAGCA CTGACAACCG GTATCATCTC CAGGCCCTTC GGCACCTCTA TGTGCTAGCT 
GCAGAACCAA GGCTCCTGGT ACCTGTGGAT GTGGATACAA ACACACCCTG CTATGCCCTT 
ATAGAAGTTA CTTACAAGGG CACTCAGTGG TATGAACAGA CCAAAGAAGA ACTGATGGCT 
CCAACCCTTC TTCCAGAACT TCATCTTTTA AAGCAGATGA AAGTTAAAGG GCCAAGATAC 
TGGGAACTGC TCATAGATTT AAGCAAGGGA GAACAGCACT TGAGGTCTAT TCTTTCGAAG 
GATGGAGTTT TATATGTAAA GCTCAGGGCA GGACAGCTCT CCTACAAAGA AGACCCAATG 
GGGTGGCAGA GTTTGTTGGC ACAAACTGTT GCTAATAGGA ACTCTGAAGC CCGGGCTTTC 
AAGCCTGAAA CAATTTCATC ATTCACTTCT GATCCAGCAC TTCTGTCATT TGCTGAATAT 
TTCTGCAAGC CGACTGTGAG CATGGGTCCA AAACAGGAGA TTCTGGACCT CTTTTCTTCG 
ATACTTTATG AGTGTGTTGC TCAGGAAACC CCAGAGATGT TGCCTGCCTA CATAGCAATG 
GATCAGGCTC TAAGAAGTCT TAAAAAGAGA GACATGTCAG ACACATCTGA TCTTTGGCAG 
ATAAAGTTGA TATTAGAATT TTTCAGTTCC CGAAGCCACC AGGATAGGCA GCATACCTAT 
CCTAAGCGAG GGCTCTTCAT AAATTCTGAG TTCCTACCTG TGGTGAAGTG CACTGTTGAT 
GCTACCCTGG ACCAGTGGCT ACAAGCTGGC GGTGATGTGT GTGTGCATGC CTACCTCAGT 
GGGCAGCCTG TTGAGAAGTC TCAGCTAAAC ATGCTGGCCT GCTTCCTTGT CTACCACTCT 
GTGCCAGCAC CACGACACTT GCCCCCCATG GGACTGGAAG GGAGCACAAG CTTTGCTGAA 
CTCCTCTACA GGTTCCGACA CCTGAAGATG CCCGTCCGGG CTTTGCTAAG ACTGGCTCCT 
GTGCTGCTTG GGAACCCACA GCCGATGGTC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237635.1
CDS	1187..4222
Translation	MASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFSCLHKFAKDFMNYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALIEVTYKGTQWYEQTKEELMAPTLLPELHLLKQMKVKGPRYWELLIDLSKGEQHLRSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISSFTSDPALLSFAEYFCKPTVSMGPKQEILDLFSSILYECVAQETPEMLPAYIAMDQALRSLKKRDMSDTSDLWQIKLILEFFSSRSHQDRQHTYPKRGLFINSEFLPVVKCTVDATLDQWLQAGGDVCVHAYLSGQPVEKSQLNMLACFLVYHSVPAPRHLPPMGLEGSTSFAELLYRFRHLKMPVRALLRLAPVLLGNPQPMVM

Target ORF information:

RefSeq Version	XM_011239333.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239333.3

ORF Insert Sequence:

ATGGCCAGTG TAGGATTCAC TTTAAGAGAT CTGGAGACTC TTCCCTTTGG GATTGCTCTT 
CCCATCAGAG ATGCGATTTA CCACTGTCGG GAGCAGCCTG ATTCAGATTG GTCAGAAGCG 
GTCTGTCTCT TGATTGGACG TCAGGATCTT TCCAAGCAGG CTTGTGAAGG AAATTTACCC 
AGAGGCAAAT CTGTGCTCTC ATCAGAGGTG TCTTCAGGAA CTGAGGCAGA GGAGGAAGAT 
GATGGCATGA ATGACTTGAA CCATGAGGTT ATGTCATTAA TATGGAGTGA AGATTTACGG 
GTGCAGGATG TGCGAAGGCT GCTTCAAAGT GCACAGCCTG TCCGTGTCAA TGTGGTGCAG 
TACCCAGAAC TCAGTGACCA TGAGTTCATT GAAGAAAAGG AAAACAGACT GCTCCAGTTG 
TGTCAGCGAA CTATGGCCCT TCCAGTAGGA CGAGGAATGT TTACCTTGTT CTCATATCAT 
CCTGTTCCAA CAGAGCCGTT GCCTGTTCCT AAATTGAATC TGACAGGGCG AGCCCCTCCA 
CGAAACACAA CTGTAGATCT TAATAGCGGA AACATCGACG TGCCTCCCAA CATGGCCAGC 
TGGGCCAGCT TTCATAATGG TGTGGCTGCC GGCCTGAAGA TAGCCCCAGC CTCCCAGATA 
GACTCAGCTT GGATCGTTTA CAACAAGCCA AAGCATGCTG AGTTAGCCAA TGAGTATGCC 
GGCTTTCTCA TGGCCCTGGG TCTGAATGGG CACCTTACCA AGCTGGCTAC TCTCAATATC 
CATGACTATT TGACCAAGGG CCATGAAATG ACAAGCATTG GATTGCTACT TGGTGTTTCT 
GCTGCAAAGC TTGGCACCAT GGACATGTCA ATTACCCGGC TCCTCAGCAT TCACGTTCCT 
GCTCTCTTAC CCCCGACATC CACAGAGCTT GACGTGCCTC ACAATGTCCA AGTGGCGGCT 
GTGGTTGGCA TTGGCCTTGT GTATCAGGGC ACAGCTCACA GACACACTGC GGAAGTCCTG 
TTGGCTGAAA TAGGGCGGCC CCCTGGTCCA GAAATGGAAT ACTGCACTGA CAGAGAGTCG 
TACTCCTTAG CTGCTGGCCT GGCCCTGGGC ATGGTTTGCT TGGGGCATGG CAGCAATTTG 
ATTGGCATGT CTGATCTCAA TGTGCCTGAG CAGCTGTATC AGTACATGGT TGGAGGCCAT 
AGGCGATTTC AAACTGGAAT GCACAGGGAG AAACATAAGT CTCCAAGTTA TCAGATCAAA 
GAAGGAGACA CCATAAACGT GGATGTGACT TGTCCCGGTG CTACTCTGGC GTTGGCTATG 
ATCTACTTAA AAACCAATAA CAGGTCCATT GCTGATTGGC TGCGTGCTCC TGATACCATG 
TATTTGCTAG ACTTTGTGAA ACCAGAATTC CTTTTGCTTA GGACACTTGC TCGGTGCTTG 
ATTTTGTGGG ATGATATCTT ACCAAATTCC AAGTGGGTTG ACAGCAATGT TCCTCAGATT 
ATAAGAGAAA ATAGTATCTC TCTGAGTGAA ATTGAATTGC CTTGTTCAGA GGACTTGAAT 
TTGGAAACCT TGTCGCAAGC ACACGTCTAC ATCATTGCAG GAGCCTGCTT GTCTCTAGGG 
TTTCGATTTG CTGGCTCAGA AAACTTATCA GCATTTAGCT GTCTGCATAA ATTTGCAAAA 
GATTTTATGA ATTATTTATC TGCACCCAAT GCTTCTGTAA CAGGGCCCTA TAACCTCGAA 
ACCTGCCTGA GTGTGGTCCT GCTGTCTCTT GCCATGGTGA TGGCTGGCTC TGGGAACCTG 
AAGGTGTTGC AGCTCTGTCG CTTTCTGCAC ATGAAGACTG GTGGAGAGAT GAACTATGGC 
TTCCACTTGG CCCACCACAT GGCCCTGGGC CTTCTCTTTT TGGGAGGAGG AAGGTACTCT 
TTGAGCACAT CCAACTCATC CATTGCTGCC CTTCTCTGCG CCCTTTACCC ACATTTCCCA 
GCCCACAGCA CTGACAACCG GTATCATCTC CAGGCCCTTC GGCACCTCTA TGTGCTAGCT 
GCAGAACCAA GGCTCCTGGT ACCTGTGGAT GTGGATACAA ACACACCCTG CTATGCCCTT 
ATAGAAGTTA CTTACAAGGG CACTCAGTGG TATGAACAGA CCAAAGAAGA ACTGATGGCT 
CCAACCCTTC TTCCAGAACT TCATCTTTTA AAGCAGATGA AAGTTAAAGG GCCAAGATAC 
TGGGAACTGC TCATAGATTT AAGCAAGGGA GAACAGCACT TGAGGTCTAT TCTTTCGAAG 
GATGGAGTTT TATATGTAAA GCTCAGGGCA GGACAGCTCT CCTACAAAGA AGACCCAATG 
GGGTGGCAGA GTTTGTTGGC ACAAACTGTT GCTAATAGGA ACTCTGAAGC CCGGGCTTTC 
AAGCCTGAAA CAATTTCATC ATTCACTTCT GATCCAGCAC TTCTGTCATT TGCTGAATAT 
TTCTGCAAGC CGACTGTGAG CATGGGTCCA AAACAGGAGA TTCTGGACCT CTTTTCTTCG 
ATACTTTATG AGTGTGTTGC TCAGGAAACC CCAGAGATGT TGCCTGCCTA CATAGCAATG 
GATCAGGCTC TAAGAAGTCT TAAAAAGAGA GACATGTCAG ACACATCTGA TCTTTGGCAG 
ATAAAGTTGA TATTAGAATT TTTCAGTTCC CGAAGCCACC AGGATAGGCA GCATACCTAT 
CCTAAGCGAG GGCTCTTCAT AAATTCTGAG TTCCTACCTG TGGTGAAGTG CACTGTTGAT 
GCTACCCTGG ACCAGTGGCT ACAAGCTGGC GGTGATGTGT GTGTGCATGC CTACCTCAGT 
GGGCAGCCTG TTGAGAAGTC TCAGCTAAAC ATGCTGGCCT GCTTCCTTGT CTACCACTCT 
GTGCCAGCAC CACGACACTT GCCCCCCATG GGACTGGAAG GGAGCACAAG CTTTGCTGAA 
CTCCTCTACA GGTTCCGACA CCTGAAGATG CCCGTCCGGG CTTTGCTAAG ACTGGCTCCT 
GTGCTGCTTG GGAACCCACA GCCGATGGTC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Anapc1 ( XM_011239332.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237634.1 Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X1, mRNA.

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CloneID	OMu68190
Clone ID Related Accession (Same CDS sequence)	XM_011239332.3 , XM_011239332.2
Accession Version	XM_011239332.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	anaphase-promoting complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239332.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACT TCTCTGAAGA AAGGGCAACG ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTTG GCCGAGACCA CTGCAAGCAC CACCCTAACG CTCTGAACCT TCAGCTTCGT 
CAGCTGCAGC CAGCCTCTGA GTTATGGTCT TCTGATGGTG CAGCTGGCTT GGTGGGATCT 
CTTCAGGAGG TTACAATCCA TGAGAAACAG AAGGAAAGCT GGCAGTTAAG AAAAGGTGTG 
AGTGAGATTG GAGATGCAGC TGACTACGAT GAAGAGCTCT ATGTGGCTGG GAACATGGTT 
ATCTGGAGCA AAGGGAGTAA AAGCCAGGCA CTGGCAGTGT ACAAAGCGTT CACAGTGGAC 
AGCACTGTCC AGCAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAG 
ATCTATAAGA GCCATGAACT AGAGAAATGC ATATGTATAT TACAGAGTTC ATGTATGAAC 
ATGCATAGCA TAGACGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTT 
TGGGCCACTA AATATGGCTT GCTGTTTGAA CGATGCAGTT CTTCACATGA GGTACCTCCA 
AGTCTACCTA GAGAACCTTT GCCTACTATG TTCAGCATGC TGCACCCACT GGATGAGATA 
ACTCCACTTG TTTGTAAATC CGGAAGTCTT TTTGGCTCAT CACGGGTACA GTATGTTGTA 
GATCCAGCAG TGAAAATTGT GTTTCTGAAC ATTGACCCCT CCATTGTCAT GACCTATGAT 
GCTGTTCAGA ATGTGCATTC TGTGTGGACT CTGCGGAGGG TAAAGCCAGA GGAAGAGAAT 
GCTGTTTTAA AGTTCCCTGA GCAGGCAGGG ACCCTGCAGA ATGCAACTAC TAGCAGCTCC 
CTGACAGCAC ATCTCAGAAG CCTCTCCAAA GGCGAATCCC CTGTGGCTTC GCCGTTCCAG 
AATTACTCTT CCATTCACAG CCAGAGTCGC TCAACCTCTT CGCCCAGTCT ACACTCTCGG 
TCGCCTTCCA TTTCCAACAT GGCGGCTCTA AGTCGTGCTC ACTCTCCTGC CTTAGGTGTG 
CACTCTTTCT CAGGGGCGCA AAGATTCAAC CTTTCAAGCC ACAGTCAGTC ACCAAAGAGA 
CACAGCATTT CTCACTCTCC GAGTGGCAGT TTCAACGACT CATTTTTGGC ACCAGAAACA 
GAGCCAATTG TTCCTGAGCT GTGCATTGAT CACTTATGGA CTGAGACACT TCCGAATATA 
AGAGAGAAAA ATTCTCAAGC CTCCAAGGTA TTTATAACGA CTGACCTGTG TGGACAGAAG 
TTCTTGTGCT TTTTAGTGGA GGCCCAGCTT CAGTTACGCT GTGTAAAGTT TCAAGAGAGT 
AATGACAAGA CTCAGCTTAT CTTTGGCTCT GTCACCAACA TACATGCAAA AGATGCAGCC 
CCAGTGGAGA AGATACACAC CATGCTGGTC CTAGAAGGCA ACGGGAACCT GGTGCTGTAC 
ACAGGAGTGG TGCGGGTGGG GAAAGTCTTT ATCCCTGGAC TTCCGGCTCC TTCCCTGACA 
ATGTCCAACA TGATGCCTCG GCCCAGTACC CCGCTCGACG GTGTTGGTAC TCCAAAGCCT 
CTGAGTAAAT TGCTTGGATC GATGGATGAG GTGGTTCTGT TGTCTCCAGT TCCAGAATTG 
AGGGATTCTT CAAAACTTAA TGATTCTCTC TACAATGAGG ATTGCACATT CCAGCAGCTT 
GGAACTTACA TTCATTCTGT GCGAGACCCT GTTCATAACA GAGTCACTCT AGAACTGAGC 
AATGGCTCCA TGGTTCGGAT CACTATCCCT GAAGTGGCCA CCTCGGAATT AGTACAAACG 
TGTCTGCAAG CAATTAAGTT CATCCTGCCA AAAGAAGTAG CCATTCAGGT GCTCGTCAAG 
TGGTACAATG TGCACAGTGC TCCAGGAGGA CCCAGCTGTC ACTCAGAGTG GAGTTTATTT 
GTGATTTGTC TCTTGAACAT GATGGGTTAT AACACAGACC GCTTAGCATG GACCCGAAGT 
TTTGACTTTG AAGGATCACT TTCCCCAGTC ATTGCACCCA AAAAAGCAAG GCCTTCTGAT 
ACTGGATCTG ACGAGGACTG GGAGTACTTA CTGAACTCAG AGTACCATCG CAATGTTGAG 
TCTCATCTTT TGAACAAATC TCTATGTTTG ACTGCTTTGG AAGTTTCAAA TGCAAAGGAT 
GAAGATTTTT CGCAGAACCT CAGTCTGGAT TCTTCTACCC TTCTCTTTGC TCACATTCCT 
GCAATTTTCT TTGTTCTTCA CCTGGTTTAT GAAGAGCTCA AGTTGAATAC TCTAATGGGA 
GAAGGAATTT GTTCTCTTAT CGACCTCCTT GTTCAGTTGG CAAGGGACTT AAAATTGGAC 
TCTTACTTGG ACCATTACTA TCGAGATTCC CCAACTCTTG TCAAAACCAC TGGACAAGTA 
TGCACCATTG ACCAAGGTCA GATGGGATTT ATGCATCATC CCCCATTTTT TACTTCTGAG 
CCACCAAGTA TTTATCAGTG GGTGAGTTCA TGCCTGAAGG GTGAAGGGAT GCCACCCTAT 
CCTTACCTTC CTGGGATTTG CGAGAGGAGC AGGCTAGTGG TCTTGAGCAT TGCCCTCTAT 
ACCCTTGGTG ATGAGAGCTG TGTGTCTGAT GAAACTTGCC AGTACTTATC CAAAGTAACC 
TCAACTCCCC AAAAGCCACA AGCAGAGCAA GAAGAAAACA GGTTTACTTT CCGGCATTCT 
GCTTCCGTTT CTGTTCTGGC CGAGAGATTA GTTGTCTGGA TGGCCAGTGT AGGATTCACT 
TTAAGAGATC TGGAGACTCT TCCCTTTGGG ATTGCTCTTC CCATCAGAGA TGCGATTTAC 
CACTGTCGGG AGCAGCCTGA TTCAGATTGG TCAGAAGCGG TCTGTCTCTT GATTGGACGT 
CAGGATCTTT CCAAGCAGGC TTGTGAAGGA AATTTACCCA GAGGCAAATC TGTGCTCTCA 
TCAGAGGTGT CTTCAGGAAC TGAGGCAGAG GAGGAAGATG ATGGCATGAA TGACTTGAAC 
CATGAGGTTA TGTCATTAAT ATGGAGTGAA GATTTACGGG TGCAGGATGT GCGAAGGCTG 
CTTCAAAGTG CACAGCCTGT CCGTGTCAAT GTGGTGCAGT ACCCAGAACT CAGTGACCAT 
GAGTTCATTG AAGAAAAGGA AAACAGACTG CTCCAGTTGT GTCAGCGAAC TATGGCCCTT 
CCAGTAGGAC GAGGAATGTT TACCTTGTTC TCATATCATC CTGTTCCAAC AGAGCCGTTG 
CCTGTTCCTA AATTGAATCT GACAGGGCGA GCCCCTCCAC GAAACACAAC TGTAGATCTT 
AATAGCGGAA ACATCGACGT GCCTCCCAAC ATGGCCAGCT GGGCCAGCTT TCATAATGGT 
GTGGCTGCCG GCCTGAAGAT AGCCCCAGCC TCCCAGATAG ACTCAGCTTG GATCGTTTAC 
AACAAGCCAA AGCATGCTGA GTTAGCCAAT GAGTATGCCG GCTTTCTCAT GGCCCTGGGT 
CTGAATGGGC ACCTTACCAA GCTGGCTACT CTCAATATCC ATGACTATTT GACCAAGGGC 
CATGAAATGA CAAGCATTGG ATTGCTACTT GGTGTTTCTG CTGCAAAGCT TGGCACCATG 
GACATGTCAA TTACCCGGCT CCTCAGCATT CACGTTCCTG CTCTCTTACC CCCGACATCC 
ACAGAGCTTG ACGTGCCTCA CAATGTCCAA GTGGCGGCTG TGGTTGGCAT TGGCCTTGTG 
TATCAGGGCA CAGCTCACAG ACACACTGCG GAAGTCCTGT TGGCTGAAAT AGGTTCTTTT 
ACCTCGAACT CTTCTCTACC GATATCCCCT CCAACCCTAC CCACAACACT AGGTAGGAGA 
GAAAGAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237634.1
CDS	268..4179
Translation	MSNFSEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGDAADYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSTVQQALWCDFIISQDKSEKIYKSHELEKCICILQSSCMNMHSIDGKDYIASLPFQVANVWATKYGLLFERCSSSHEVPPSLPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKPEEENAVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFNDSFLAPETEPIVPELCIDHLWTETLPNIREKNSQASKVFITTDLCGQKFLCFLVEAQLQLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLVLEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQAIKFILPKEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSFDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHRNVESHLLNKSLCLTALEVSNAKDEDFSQNLSLDSSTLLFAHIPAIFFVLHLVYEELKLNTLMGEGICSLIDLLVQLARDLKLDSYLDHYYRDSPTLVKTTGQVCTIDQGQMGFMHHPPFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYTLGDESCVSDETCQYLSKVTSTPQKPQAEQEENRFTFRHSASVSVLAERLVVWMASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGSFTSNSSLPISPPTLPTTLGRRERR

Target ORF information:

RefSeq Version	XM_011239332.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239332.2

ORF Insert Sequence:

ATGTCGAACT TCTCTGAAGA AAGGGCAACG ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTTG GCCGAGACCA CTGCAAGCAC CACCCTAACG CTCTGAACCT TCAGCTTCGT 
CAGCTGCAGC CAGCCTCTGA GTTATGGTCT TCTGATGGTG CAGCTGGCTT GGTGGGATCT 
CTTCAGGAGG TTACAATCCA TGAGAAACAG AAGGAAAGCT GGCAGTTAAG AAAAGGTGTG 
AGTGAGATTG GAGATGCAGC TGACTACGAT GAAGAGCTCT ATGTGGCTGG GAACATGGTT 
ATCTGGAGCA AAGGGAGTAA AAGCCAGGCA CTGGCAGTGT ACAAAGCGTT CACAGTGGAC 
AGCACTGTCC AGCAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAG 
ATCTATAAGA GCCATGAACT AGAGAAATGC ATATGTATAT TACAGAGTTC ATGTATGAAC 
ATGCATAGCA TAGACGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTT 
TGGGCCACTA AATATGGCTT GCTGTTTGAA CGATGCAGTT CTTCACATGA GGTACCTCCA 
AGTCTACCTA GAGAACCTTT GCCTACTATG TTCAGCATGC TGCACCCACT GGATGAGATA 
ACTCCACTTG TTTGTAAATC CGGAAGTCTT TTTGGCTCAT CACGGGTACA GTATGTTGTA 
GATCCAGCAG TGAAAATTGT GTTTCTGAAC ATTGACCCCT CCATTGTCAT GACCTATGAT 
GCTGTTCAGA ATGTGCATTC TGTGTGGACT CTGCGGAGGG TAAAGCCAGA GGAAGAGAAT 
GCTGTTTTAA AGTTCCCTGA GCAGGCAGGG ACCCTGCAGA ATGCAACTAC TAGCAGCTCC 
CTGACAGCAC ATCTCAGAAG CCTCTCCAAA GGCGAATCCC CTGTGGCTTC GCCGTTCCAG 
AATTACTCTT CCATTCACAG CCAGAGTCGC TCAACCTCTT CGCCCAGTCT ACACTCTCGG 
TCGCCTTCCA TTTCCAACAT GGCGGCTCTA AGTCGTGCTC ACTCTCCTGC CTTAGGTGTG 
CACTCTTTCT CAGGGGCGCA AAGATTCAAC CTTTCAAGCC ACAGTCAGTC ACCAAAGAGA 
CACAGCATTT CTCACTCTCC GAGTGGCAGT TTCAACGACT CATTTTTGGC ACCAGAAACA 
GAGCCAATTG TTCCTGAGCT GTGCATTGAT CACTTATGGA CTGAGACACT TCCGAATATA 
AGAGAGAAAA ATTCTCAAGC CTCCAAGGTA TTTATAACGA CTGACCTGTG TGGACAGAAG 
TTCTTGTGCT TTTTAGTGGA GGCCCAGCTT CAGTTACGCT GTGTAAAGTT TCAAGAGAGT 
AATGACAAGA CTCAGCTTAT CTTTGGCTCT GTCACCAACA TACATGCAAA AGATGCAGCC 
CCAGTGGAGA AGATACACAC CATGCTGGTC CTAGAAGGCA ACGGGAACCT GGTGCTGTAC 
ACAGGAGTGG TGCGGGTGGG GAAAGTCTTT ATCCCTGGAC TTCCGGCTCC TTCCCTGACA 
ATGTCCAACA TGATGCCTCG GCCCAGTACC CCGCTCGACG GTGTTGGTAC TCCAAAGCCT 
CTGAGTAAAT TGCTTGGATC GATGGATGAG GTGGTTCTGT TGTCTCCAGT TCCAGAATTG 
AGGGATTCTT CAAAACTTAA TGATTCTCTC TACAATGAGG ATTGCACATT CCAGCAGCTT 
GGAACTTACA TTCATTCTGT GCGAGACCCT GTTCATAACA GAGTCACTCT AGAACTGAGC 
AATGGCTCCA TGGTTCGGAT CACTATCCCT GAAGTGGCCA CCTCGGAATT AGTACAAACG 
TGTCTGCAAG CAATTAAGTT CATCCTGCCA AAAGAAGTAG CCATTCAGGT GCTCGTCAAG 
TGGTACAATG TGCACAGTGC TCCAGGAGGA CCCAGCTGTC ACTCAGAGTG GAGTTTATTT 
GTGATTTGTC TCTTGAACAT GATGGGTTAT AACACAGACC GCTTAGCATG GACCCGAAGT 
TTTGACTTTG AAGGATCACT TTCCCCAGTC ATTGCACCCA AAAAAGCAAG GCCTTCTGAT 
ACTGGATCTG ACGAGGACTG GGAGTACTTA CTGAACTCAG AGTACCATCG CAATGTTGAG 
TCTCATCTTT TGAACAAATC TCTATGTTTG ACTGCTTTGG AAGTTTCAAA TGCAAAGGAT 
GAAGATTTTT CGCAGAACCT CAGTCTGGAT TCTTCTACCC TTCTCTTTGC TCACATTCCT 
GCAATTTTCT TTGTTCTTCA CCTGGTTTAT GAAGAGCTCA AGTTGAATAC TCTAATGGGA 
GAAGGAATTT GTTCTCTTAT CGACCTCCTT GTTCAGTTGG CAAGGGACTT AAAATTGGAC 
TCTTACTTGG ACCATTACTA TCGAGATTCC CCAACTCTTG TCAAAACCAC TGGACAAGTA 
TGCACCATTG ACCAAGGTCA GATGGGATTT ATGCATCATC CCCCATTTTT TACTTCTGAG 
CCACCAAGTA TTTATCAGTG GGTGAGTTCA TGCCTGAAGG GTGAAGGGAT GCCACCCTAT 
CCTTACCTTC CTGGGATTTG CGAGAGGAGC AGGCTAGTGG TCTTGAGCAT TGCCCTCTAT 
ACCCTTGGTG ATGAGAGCTG TGTGTCTGAT GAAACTTGCC AGTACTTATC CAAAGTAACC 
TCAACTCCCC AAAAGCCACA AGCAGAGCAA GAAGAAAACA GGTTTACTTT CCGGCATTCT 
GCTTCCGTTT CTGTTCTGGC CGAGAGATTA GTTGTCTGGA TGGCCAGTGT AGGATTCACT 
TTAAGAGATC TGGAGACTCT TCCCTTTGGG ATTGCTCTTC CCATCAGAGA TGCGATTTAC 
CACTGTCGGG AGCAGCCTGA TTCAGATTGG TCAGAAGCGG TCTGTCTCTT GATTGGACGT 
CAGGATCTTT CCAAGCAGGC TTGTGAAGGA AATTTACCCA GAGGCAAATC TGTGCTCTCA 
TCAGAGGTGT CTTCAGGAAC TGAGGCAGAG GAGGAAGATG ATGGCATGAA TGACTTGAAC 
CATGAGGTTA TGTCATTAAT ATGGAGTGAA GATTTACGGG TGCAGGATGT GCGAAGGCTG 
CTTCAAAGTG CACAGCCTGT CCGTGTCAAT GTGGTGCAGT ACCCAGAACT CAGTGACCAT 
GAGTTCATTG AAGAAAAGGA AAACAGACTG CTCCAGTTGT GTCAGCGAAC TATGGCCCTT 
CCAGTAGGAC GAGGAATGTT TACCTTGTTC TCATATCATC CTGTTCCAAC AGAGCCGTTG 
CCTGTTCCTA AATTGAATCT GACAGGGCGA GCCCCTCCAC GAAACACAAC TGTAGATCTT 
AATAGCGGAA ACATCGACGT GCCTCCCAAC ATGGCCAGCT GGGCCAGCTT TCATAATGGT 
GTGGCTGCCG GCCTGAAGAT AGCCCCAGCC TCCCAGATAG ACTCAGCTTG GATCGTTTAC 
AACAAGCCAA AGCATGCTGA GTTAGCCAAT GAGTATGCCG GCTTTCTCAT GGCCCTGGGT 
CTGAATGGGC ACCTTACCAA GCTGGCTACT CTCAATATCC ATGACTATTT GACCAAGGGC 
CATGAAATGA CAAGCATTGG ATTGCTACTT GGTGTTTCTG CTGCAAAGCT TGGCACCATG 
GACATGTCAA TTACCCGGCT CCTCAGCATT CACGTTCCTG CTCTCTTACC CCCGACATCC 
ACAGAGCTTG ACGTGCCTCA CAATGTCCAA GTGGCGGCTG TGGTTGGCAT TGGCCTTGTG 
TATCAGGGCA CAGCTCACAG ACACACTGCG GAAGTCCTGT TGGCTGAAAT AGGTTCTTTT 
ACCTCGAACT CTTCTCTACC GATATCCCCT CCAACCCTAC CCACAACACT AGGTAGGAGA 
GAAAGAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Anapc1 ( XM_011239333.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237635.1 Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu68191
Clone ID Related Accession (Same CDS sequence)	XM_011239333.3 , XM_011239333.2
Accession Version	XM_011239333.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	anaphase-promoting complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTG TAGGATTCAC TTTAAGAGAT CTGGAGACTC TTCCCTTTGG GATTGCTCTT 
CCCATCAGAG ATGCGATTTA CCACTGTCGG GAGCAGCCTG ATTCAGATTG GTCAGAAGCG 
GTCTGTCTCT TGATTGGACG TCAGGATCTT TCCAAGCAGG CTTGTGAAGG AAATTTACCC 
AGAGGCAAAT CTGTGCTCTC ATCAGAGGTG TCTTCAGGAA CTGAGGCAGA GGAGGAAGAT 
GATGGCATGA ATGACTTGAA CCATGAGGTT ATGTCATTAA TATGGAGTGA AGATTTACGG 
GTGCAGGATG TGCGAAGGCT GCTTCAAAGT GCACAGCCTG TCCGTGTCAA TGTGGTGCAG 
TACCCAGAAC TCAGTGACCA TGAGTTCATT GAAGAAAAGG AAAACAGACT GCTCCAGTTG 
TGTCAGCGAA CTATGGCCCT TCCAGTAGGA CGAGGAATGT TTACCTTGTT CTCATATCAT 
CCTGTTCCAA CAGAGCCGTT GCCTGTTCCT AAATTGAATC TGACAGGGCG AGCCCCTCCA 
CGAAACACAA CTGTAGATCT TAATAGCGGA AACATCGACG TGCCTCCCAA CATGGCCAGC 
TGGGCCAGCT TTCATAATGG TGTGGCTGCC GGCCTGAAGA TAGCCCCAGC CTCCCAGATA 
GACTCAGCTT GGATCGTTTA CAACAAGCCA AAGCATGCTG AGTTAGCCAA TGAGTATGCC 
GGCTTTCTCA TGGCCCTGGG TCTGAATGGG CACCTTACCA AGCTGGCTAC TCTCAATATC 
CATGACTATT TGACCAAGGG CCATGAAATG ACAAGCATTG GATTGCTACT TGGTGTTTCT 
GCTGCAAAGC TTGGCACCAT GGACATGTCA ATTACCCGGC TCCTCAGCAT TCACGTTCCT 
GCTCTCTTAC CCCCGACATC CACAGAGCTT GACGTGCCTC ACAATGTCCA AGTGGCGGCT 
GTGGTTGGCA TTGGCCTTGT GTATCAGGGC ACAGCTCACA GACACACTGC GGAAGTCCTG 
TTGGCTGAAA TAGGGCGGCC CCCTGGTCCA GAAATGGAAT ACTGCACTGA CAGAGAGTCG 
TACTCCTTAG CTGCTGGCCT GGCCCTGGGC ATGGTTTGCT TGGGGCATGG CAGCAATTTG 
ATTGGCATGT CTGATCTCAA TGTGCCTGAG CAGCTGTATC AGTACATGGT TGGAGGCCAT 
AGGCGATTTC AAACTGGAAT GCACAGGGAG AAACATAAGT CTCCAAGTTA TCAGATCAAA 
GAAGGAGACA CCATAAACGT GGATGTGACT TGTCCCGGTG CTACTCTGGC GTTGGCTATG 
ATCTACTTAA AAACCAATAA CAGGTCCATT GCTGATTGGC TGCGTGCTCC TGATACCATG 
TATTTGCTAG ACTTTGTGAA ACCAGAATTC CTTTTGCTTA GGACACTTGC TCGGTGCTTG 
ATTTTGTGGG ATGATATCTT ACCAAATTCC AAGTGGGTTG ACAGCAATGT TCCTCAGATT 
ATAAGAGAAA ATAGTATCTC TCTGAGTGAA ATTGAATTGC CTTGTTCAGA GGACTTGAAT 
TTGGAAACCT TGTCGCAAGC ACACGTCTAC ATCATTGCAG GAGCCTGCTT GTCTCTAGGG 
TTTCGATTTG CTGGCTCAGA AAACTTATCA GCATTTAGCT GTCTGCATAA ATTTGCAAAA 
GATTTTATGA ATTATTTATC TGCACCCAAT GCTTCTGTAA CAGGGCCCTA TAACCTCGAA 
ACCTGCCTGA GTGTGGTCCT GCTGTCTCTT GCCATGGTGA TGGCTGGCTC TGGGAACCTG 
AAGGTGTTGC AGCTCTGTCG CTTTCTGCAC ATGAAGACTG GTGGAGAGAT GAACTATGGC 
TTCCACTTGG CCCACCACAT GGCCCTGGGC CTTCTCTTTT TGGGAGGAGG AAGGTACTCT 
TTGAGCACAT CCAACTCATC CATTGCTGCC CTTCTCTGCG CCCTTTACCC ACATTTCCCA 
GCCCACAGCA CTGACAACCG GTATCATCTC CAGGCCCTTC GGCACCTCTA TGTGCTAGCT 
GCAGAACCAA GGCTCCTGGT ACCTGTGGAT GTGGATACAA ACACACCCTG CTATGCCCTT 
ATAGAAGTTA CTTACAAGGG CACTCAGTGG TATGAACAGA CCAAAGAAGA ACTGATGGCT 
CCAACCCTTC TTCCAGAACT TCATCTTTTA AAGCAGATGA AAGTTAAAGG GCCAAGATAC 
TGGGAACTGC TCATAGATTT AAGCAAGGGA GAACAGCACT TGAGGTCTAT TCTTTCGAAG 
GATGGAGTTT TATATGTAAA GCTCAGGGCA GGACAGCTCT CCTACAAAGA AGACCCAATG 
GGGTGGCAGA GTTTGTTGGC ACAAACTGTT GCTAATAGGA ACTCTGAAGC CCGGGCTTTC 
AAGCCTGAAA CAATTTCATC ATTCACTTCT GATCCAGCAC TTCTGTCATT TGCTGAATAT 
TTCTGCAAGC CGACTGTGAG CATGGGTCCA AAACAGGAGA TTCTGGACCT CTTTTCTTCG 
ATACTTTATG AGTGTGTTGC TCAGGAAACC CCAGAGATGT TGCCTGCCTA CATAGCAATG 
GATCAGGCTC TAAGAAGTCT TAAAAAGAGA GACATGTCAG ACACATCTGA TCTTTGGCAG 
ATAAAGTTGA TATTAGAATT TTTCAGTTCC CGAAGCCACC AGGATAGGCA GCATACCTAT 
CCTAAGCGAG GGCTCTTCAT AAATTCTGAG TTCCTACCTG TGGTGAAGTG CACTGTTGAT 
GCTACCCTGG ACCAGTGGCT ACAAGCTGGC GGTGATGTGT GTGTGCATGC CTACCTCAGT 
GGGCAGCCTG TTGAGAAGTC TCAGCTAAAC ATGCTGGCCT GCTTCCTTGT CTACCACTCT 
GTGCCAGCAC CACGACACTT GCCCCCCATG GGACTGGAAG GGAGCACAAG CTTTGCTGAA 
CTCCTCTACA GGTTCCGACA CCTGAAGATG CCCGTCCGGG CTTTGCTAAG ACTGGCTCCT 
GTGCTGCTTG GGAACCCACA GCCGATGGTC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237635.1
CDS	1186..4221
Translation	MASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFSCLHKFAKDFMNYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALIEVTYKGTQWYEQTKEELMAPTLLPELHLLKQMKVKGPRYWELLIDLSKGEQHLRSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISSFTSDPALLSFAEYFCKPTVSMGPKQEILDLFSSILYECVAQETPEMLPAYIAMDQALRSLKKRDMSDTSDLWQIKLILEFFSSRSHQDRQHTYPKRGLFINSEFLPVVKCTVDATLDQWLQAGGDVCVHAYLSGQPVEKSQLNMLACFLVYHSVPAPRHLPPMGLEGSTSFAELLYRFRHLKMPVRALLRLAPVLLGNPQPMVM

Target ORF information:

RefSeq Version	XM_011239333.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239333.2

ORF Insert Sequence:

ATGGCCAGTG TAGGATTCAC TTTAAGAGAT CTGGAGACTC TTCCCTTTGG GATTGCTCTT 
CCCATCAGAG ATGCGATTTA CCACTGTCGG GAGCAGCCTG ATTCAGATTG GTCAGAAGCG 
GTCTGTCTCT TGATTGGACG TCAGGATCTT TCCAAGCAGG CTTGTGAAGG AAATTTACCC 
AGAGGCAAAT CTGTGCTCTC ATCAGAGGTG TCTTCAGGAA CTGAGGCAGA GGAGGAAGAT 
GATGGCATGA ATGACTTGAA CCATGAGGTT ATGTCATTAA TATGGAGTGA AGATTTACGG 
GTGCAGGATG TGCGAAGGCT GCTTCAAAGT GCACAGCCTG TCCGTGTCAA TGTGGTGCAG 
TACCCAGAAC TCAGTGACCA TGAGTTCATT GAAGAAAAGG AAAACAGACT GCTCCAGTTG 
TGTCAGCGAA CTATGGCCCT TCCAGTAGGA CGAGGAATGT TTACCTTGTT CTCATATCAT 
CCTGTTCCAA CAGAGCCGTT GCCTGTTCCT AAATTGAATC TGACAGGGCG AGCCCCTCCA 
CGAAACACAA CTGTAGATCT TAATAGCGGA AACATCGACG TGCCTCCCAA CATGGCCAGC 
TGGGCCAGCT TTCATAATGG TGTGGCTGCC GGCCTGAAGA TAGCCCCAGC CTCCCAGATA 
GACTCAGCTT GGATCGTTTA CAACAAGCCA AAGCATGCTG AGTTAGCCAA TGAGTATGCC 
GGCTTTCTCA TGGCCCTGGG TCTGAATGGG CACCTTACCA AGCTGGCTAC TCTCAATATC 
CATGACTATT TGACCAAGGG CCATGAAATG ACAAGCATTG GATTGCTACT TGGTGTTTCT 
GCTGCAAAGC TTGGCACCAT GGACATGTCA ATTACCCGGC TCCTCAGCAT TCACGTTCCT 
GCTCTCTTAC CCCCGACATC CACAGAGCTT GACGTGCCTC ACAATGTCCA AGTGGCGGCT 
GTGGTTGGCA TTGGCCTTGT GTATCAGGGC ACAGCTCACA GACACACTGC GGAAGTCCTG 
TTGGCTGAAA TAGGGCGGCC CCCTGGTCCA GAAATGGAAT ACTGCACTGA CAGAGAGTCG 
TACTCCTTAG CTGCTGGCCT GGCCCTGGGC ATGGTTTGCT TGGGGCATGG CAGCAATTTG 
ATTGGCATGT CTGATCTCAA TGTGCCTGAG CAGCTGTATC AGTACATGGT TGGAGGCCAT 
AGGCGATTTC AAACTGGAAT GCACAGGGAG AAACATAAGT CTCCAAGTTA TCAGATCAAA 
GAAGGAGACA CCATAAACGT GGATGTGACT TGTCCCGGTG CTACTCTGGC GTTGGCTATG 
ATCTACTTAA AAACCAATAA CAGGTCCATT GCTGATTGGC TGCGTGCTCC TGATACCATG 
TATTTGCTAG ACTTTGTGAA ACCAGAATTC CTTTTGCTTA GGACACTTGC TCGGTGCTTG 
ATTTTGTGGG ATGATATCTT ACCAAATTCC AAGTGGGTTG ACAGCAATGT TCCTCAGATT 
ATAAGAGAAA ATAGTATCTC TCTGAGTGAA ATTGAATTGC CTTGTTCAGA GGACTTGAAT 
TTGGAAACCT TGTCGCAAGC ACACGTCTAC ATCATTGCAG GAGCCTGCTT GTCTCTAGGG 
TTTCGATTTG CTGGCTCAGA AAACTTATCA GCATTTAGCT GTCTGCATAA ATTTGCAAAA 
GATTTTATGA ATTATTTATC TGCACCCAAT GCTTCTGTAA CAGGGCCCTA TAACCTCGAA 
ACCTGCCTGA GTGTGGTCCT GCTGTCTCTT GCCATGGTGA TGGCTGGCTC TGGGAACCTG 
AAGGTGTTGC AGCTCTGTCG CTTTCTGCAC ATGAAGACTG GTGGAGAGAT GAACTATGGC 
TTCCACTTGG CCCACCACAT GGCCCTGGGC CTTCTCTTTT TGGGAGGAGG AAGGTACTCT 
TTGAGCACAT CCAACTCATC CATTGCTGCC CTTCTCTGCG CCCTTTACCC ACATTTCCCA 
GCCCACAGCA CTGACAACCG GTATCATCTC CAGGCCCTTC GGCACCTCTA TGTGCTAGCT 
GCAGAACCAA GGCTCCTGGT ACCTGTGGAT GTGGATACAA ACACACCCTG CTATGCCCTT 
ATAGAAGTTA CTTACAAGGG CACTCAGTGG TATGAACAGA CCAAAGAAGA ACTGATGGCT 
CCAACCCTTC TTCCAGAACT TCATCTTTTA AAGCAGATGA AAGTTAAAGG GCCAAGATAC 
TGGGAACTGC TCATAGATTT AAGCAAGGGA GAACAGCACT TGAGGTCTAT TCTTTCGAAG 
GATGGAGTTT TATATGTAAA GCTCAGGGCA GGACAGCTCT CCTACAAAGA AGACCCAATG 
GGGTGGCAGA GTTTGTTGGC ACAAACTGTT GCTAATAGGA ACTCTGAAGC CCGGGCTTTC 
AAGCCTGAAA CAATTTCATC ATTCACTTCT GATCCAGCAC TTCTGTCATT TGCTGAATAT 
TTCTGCAAGC CGACTGTGAG CATGGGTCCA AAACAGGAGA TTCTGGACCT CTTTTCTTCG 
ATACTTTATG AGTGTGTTGC TCAGGAAACC CCAGAGATGT TGCCTGCCTA CATAGCAATG 
GATCAGGCTC TAAGAAGTCT TAAAAAGAGA GACATGTCAG ACACATCTGA TCTTTGGCAG 
ATAAAGTTGA TATTAGAATT TTTCAGTTCC CGAAGCCACC AGGATAGGCA GCATACCTAT 
CCTAAGCGAG GGCTCTTCAT AAATTCTGAG TTCCTACCTG TGGTGAAGTG CACTGTTGAT 
GCTACCCTGG ACCAGTGGCT ACAAGCTGGC GGTGATGTGT GTGTGCATGC CTACCTCAGT 
GGGCAGCCTG TTGAGAAGTC TCAGCTAAAC ATGCTGGCCT GCTTCCTTGT CTACCACTCT 
GTGCCAGCAC CACGACACTT GCCCCCCATG GGACTGGAAG GGAGCACAAG CTTTGCTGAA 
CTCCTCTACA GGTTCCGACA CCTGAAGATG CCCGTCCGGG CTTTGCTAAG ACTGGCTCCT 
GTGCTGCTTG GGAACCCACA GCCGATGGTC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Anapc1 ( XM_030248086.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103946.1 Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87737
Clone ID Related Accession (Same CDS sequence)	XM_030248086.1
Accession Version	XM_030248086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	anaphase-promoting complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTGA CTACGATGAA GAGCTCTATG TGGCTGGGAA CATGGTTATC TGGAGCAAAG 
GGAGTAAAAG CCAGGCACTG GCAGTGTACA AAGCGTTCAC AGTGGACAGC ACTGTCCAGC 
AGGCATTGTG GTGTGACTTC ATTATATCAC AGGATAAGTC TGAAAAGATC TATAGTGGCA 
AATGTTTGGG CCACTAAATA TGGCTTGCTG TTTGAACGAT GCAGTTCTTC ACATGAGGTA 
CCTCCAAGTC TACCTAGAGA ACCTTTGCCT ACTATGTTCA GCATGCTGCA CCCACTGGAT 
GAGATAACTC CACTTGTTTG TAAATCCGGA AGTCTTTTTG GCTCATCACG GGTACAGTAT 
GTTGTAGATC CAGCAGTGAA AATTGTGTTT CTGAACATTG ACCCCTCCAT TGTCATGACC 
TATGATGCTG TTCAGAATGT GCATTCTGTG TGGACTCTGC GGAGGGTAAA GCCAGAGGAA 
GAGAATGCTG TTTTAAAGTT CCCTGAGCAG GCAGGGACCC TGCAGAATGC AACTACTAGC 
AGCTCCCTGA CAGCACATCT CAGAAGCCTC TCCAAAGGCG AATCCCCTGT GGCTTCGCCG 
TTCCAGAATT ACTCTTCCAT TCACAGCCAG AGTCGCTCAA CCTCTTCGCC CAGTCTACAC 
TCTCGGTCGC CTTCCATTTC CAACATGGCG GCTCTAAGTC GTGCTCACTC TCCTGCCTTA 
GGTGTGCACT CTTTCTCAGG GGCGCAAAGA TTCAACCTTT CAAGCCACAG TCAGTCACCA 
AAGAGACACA GCATTTCTCA CTCTCCGAGT GGCAGTTTCA ACGACTCATT TTTGGCACCA 
GAAACAGAGC CAATTGTTCC TGAGCTGTGC ATTGATCACT TATGGACTGA GACACTTCCG 
AATATAAGAG AGAAAAATTC TCAAGCCTCC AAGGTATTTA TAACGACTGA CCTGTGTGGA 
CAGAAGTTCT TGTGCTTTTT AGTGGAGGCC CAGCTTCAGT TACGCTGTGT AAAGTTTCAA 
GAGAGTAATG ACAAGACTCA GCTTATCTTT GGCTCTGTCA CCAACATACA TGCAAAAGAT 
GCAGCCCCAG TGGAGAAGAT ACACACCATG CTGGTCCTAG AAGGCAACGG GAACCTGGTG 
CTGTACACAG GAGTGGTGCG GGTGGGGAAA GTCTTTATCC CTGGACTTCC GGCTCCTTCC 
CTGACAATGT CCAACATGAT GCCTCGGCCC AGTACCCCGC TCGACGGTGT TGGTACTCCA 
AAGCCTCTGA GTAAATTGCT TGGATCGATG GATGAGGTGG TTCTGTTGTC TCCAGTTCCA 
GAATTGAGGG ATTCTTCAAA ACTTAATGAT TCTCTCTACA ATGAGGATTG CACATTCCAG 
CAGCTTGGAA CTTACATTCA TTCTGTGCGA GACCCTGTTC ATAACAGAGT CACTCTAGAA 
CTGAGCAATG GCTCCATGGT TCGGATCACT ATCCCTGAAG TGGCCACCTC GGAATTAGTA 
CAAACGTGTC TGCAAGCAAT TAAGTTCATC CTGCCAAAAG AAGTAGCCAT TCAGGTGCTC 
GTCAAGTGGT ACAATGTGCA CAGTGCTCCA GGAGGACCCA GCTGTCACTC AGAGTGGAGT 
TTATTTGTGA TTTGTCTCTT GAACATGATG GGTTATAACA CAGACCGCTT AGCATGGACC 
CGAAGTTTTG ACTTTGAAGG ATCACTTTCC CCAGTCATTG CACCCAAAAA AGCAAGGCCT 
TCTGATACTG GATCTGACGA GGACTGGGAG TACTTACTGA ACTCAGAGTA CCATCGCAAT 
GTTGAGTCTC ATCTTTTGAA CAAATCTCTA TGTTTGACTG CTTTGGAAGT TTCAAATGCA 
AAGGATGAAG ATTTTTCGCA GAACCTCAGT CTGGATTCTT CTACCCTTCT CTTTGCTCAC 
ATTCCTGCAA TTTTCTTTGT TCTTCACCTG GTTTATGAAG AGCTCAAGTT GAATACTCTA 
ATGGGAGAAG GAATTTGTTC TCTTATCGAC CTCCTTGTTC AGTTGGCAAG GGACTTAAAA 
TTGGACTCTT ACTTGGACCA TTACTATCGA GATTCCCCAA CTCTTGTCAA AACCACTGGA 
CAAGTATGCA CCATTGACCA AGGTCAGATG GGATTTATGC ATCATCCCCC ATTTTTTACT 
TCTGAGCCAC CAAGTATTTA TCAGTGGGTG AGTTCATGCC TGAAGGGTGA AGGGATGCCA 
CCCTATCCTT ACCTTCCTGG GATTTGCGAG AGGAGCAGGC TAGTGGTCTT GAGCATTGCC 
CTCTATACCC TTGGTGATGA GAGCTGTGTG TCTGATGAAA CTTGCCAGTA CTTATCCAAA 
GTAACCTCAA CTCCCCAAAA GCCACAAGCA GAGCAAGAAG AAAACAGGTT TACTTTCCGG 
CATTCTGCTT CCGTTTCTGT TCTGGCCGAG AGATTAGTTG TCTGGATGGC CAGTGTAGGA 
TTCACTTTAA GAGATCTGGA GACTCTTCCC TTTGGGATTG CTCTTCCCAT CAGAGATGCG 
ATTTACCACT GTCGGGAGCA GCCTGATTCA GATTGGTCAG AAGCGGTCTG TCTCTTGATT 
GGACGTCAGG ATCTTTCCAA GCAGGCTTGT GAAGGAAATT TACCCAGAGG CAAATCTGTG 
CTCTCATCAG AGGTGTCTTC AGGAACTGAG GCAGAGGAGG AAGATGATGG CATGAATGAC 
TTGAACCATG AGGTTATGTC ATTAATATGG AGTGAAGATT TACGGGTGCA GGATGTGCGA 
AGGCTGCTTC AAAGTGCACA GCCTGTCCGT GTCAATGTGG TGCAGTACCC AGAACTCAGT 
GACCATGAGT TCATTGAAGA AAAGGAAAAC AGACTGCTCC AGTTGTGTCA GCGAACTATG 
GCCCTTCCAG TAGGACGAGG AATGTTTACC TTGTTCTCAT ATCATCCTGT TCCAACAGAG 
CCGTTGCCTG TTCCTAAATT GAATCTGACA GGGCGAGCCC CTCCACGAAA CACAACTGTA 
GATCTTAATA GCGGAAACAT CGACGTGCCT CCCAACATGG CCAGCTGGGC CAGCTTTCAT 
AATGGTGTGG CTGCCGGCCT GAAGATAGCC CCAGCCTCCC AGATAGACTC AGCTTGGATC 
GTTTACAACA AGCCAAAGCA TGCTGAGTTA GCCAATGAGT ATGCCGGCTT TCTCATGGCC 
CTGGGTCTGA ATGGGCACCT TACCAAGCTG GCTACTCTCA ATATCCATGA CTATTTGACC 
AAGGGCCATG AAATGACAAG CATTGGATTG CTACTTGGTG TTTCTGCTGC AAAGCTTGGC 
ACCATGGACA TGTCAATTAC CCGGCTCCTC AGCATTCACG TTCCTGCTCT CTTACCCCCG 
ACATCCACAG AGCTTGACGT GCCTCACAAT GTCCAAGTGG CGGCTGTGGT TGGCATTGGC 
CTTGTGTATC AGGGCACAGC TCACAGACAC ACTGCGGAAG TCCTGTTGGC TGAAATAGGG 
CGGCCCCCTG GTCCAGAAAT GGAATACTGC ACTGACAGAG AGTCGTACTC CTTAGCTGCT 
GGCCTGGCCC TGGGCATGGT TTGCTTGGGG CATGGCAGCA ATTTGATTGG CATGTCTGAT 
CTCAATGTGC CTGAGCAGCT GTATCAGTAC ATGGTTGGAG GCCATAGGCG ATTTCAAACT 
GGAATGCACA GGGAGAAACA TAAGTCTCCA AGTTATCAGA TCAAAGAAGG AGACACCATA 
AACGTGGATG TGACTTGTCC CGGTGCTACT CTGGCGTTGG CTATGATCTA CTTAAAAACC 
AATAACAGGT CCATTGCTGA TTGGCTGCGT GCTCCTGATA CCATGTATTT GCTAGACTTT 
GTGAAACCAG AATTCCTTTT GCTTAGGACA CTTGCTCGGT GCTTGATTTT GTGGGATGAT 
ATCTTACCAA ATTCCAAGTG GGTTGACAGC AATGTTCCTC AGATTATAAG AGAAAATAGT 
ATCTCTCTGA GTGAAATTGA ATTGCCTTGT TCAGAGGACT TGAATTTGGA AACCTTGTCG 
CAAGCACACG TCTACATCAT TGCAGGAGCC TGCTTGTCTC TAGGGTTTCG ATTTGCTGGC 
TCAGAAAACT TATCAGCATT TAGCTGTCTG CATAAATTTG CAAAAGATTT TATGAATTAT 
TTATCTGCAC CCAATGCTTC TGTAACAGGG CCCTATAACC TCGAAACCTG CCTGAGTGTG 
GTCCTGCTGT CTCTTGCCAT GGTGATGGCT GGCTCTGGGA ACCTGAAGGT GTTGCAGCTC 
TGTCGCTTTC TGCACATGAA GACTGGTGGA GAGATGAACT ATGGCTTCCA CTTGGCCCAC 
CACATGGCCC TGGGCCTTCT CTTTTTGGGA GGAGGAAGGT ACTCTTTGAG CACATCCAAC 
TCATCCATTG CTGCCCTTCT CTGCGCCCTT TACCCACATT TCCCAGCCCA CAGCACTGAC 
AACCGGTATC ATCTCCAGGC CCTTCGGCAC CTCTATGTGC TAGCTGCAGA ACCAAGGCTC 
CTGGTACCTG TGGATGTGGA TACAAACACA CCCTGCTATG CCCTTATAGA AGTTACTTAC 
AAGGGCACTC AGTGGTATGA ACAGACCAAA GAAGAACTGA TGGCTCCAAC CCTTCTTCCA 
GAACTTCATC TTTTAAAGCA GATGAAAGTT AAAGGGCCAA GATACTGGGA ACTGCTCATA 
GATTTAAGCA AGGGAGAACA GCACTTGAGG TCTATTCTTT CGAAGGATGG AGTTTTATAT 
GTAAAGCTCA GGGCAGGACA GCTCTCCTAC AAAGAAGACC CAATGGGGTG GCAGAGTTTG 
TTGGCACAAA CTGTTGCTAA TAGGAACTCT GAAGCCCGGG CTTTCAAGCC TGAAACAATT 
TCATCATTCA CTTCTGATCC AGCACTTCTG TCATTTGCTG AATATTTCTG CAAGCCGACT 
GTGAGCATGG GTCCAAAACA GGAGATTCTG GACCTCTTTT CTTCGATACT TTATGAGTGT 
GTTGCTCAGG AAACCCCAGA GATGTTGCCT GCCTACATAG CAATGGATCA GGCTCTAAGA 
AGTCTTAAAA AGAGAGACAT GTCAGACACA TCTGATCTTT GGCAGATAAA GTTGATATTA 
GAATTTTTCA GTTCCCGAAG CCACCAGGAT AGGCAGCATA CCTATCCTAA GCGAGGGCTC 
TTCATAAATT CTGAGTTCCT ACCTGTGGTG AAGTGCACTG TTGATGCTAC CCTGGACCAG 
TGGCTACAAG CTGGCGGTGA TGTGTGTGTG CATGCCTACC TCAGTGGGCA GCCTGTTGAG 
AAGTCTCAGC TAAACATGCT GGCCTGCTTC CTTGTCTACC ACTCTGTGCC AGCACCACGA 
CACTTGCCCC CCATGGGACT GGAAGGGAGC ACAAGCTTTG CTGAACTCCT CTACAGGTTC 
CGACACCTGA AGATGCCCGT CCGGGCTTTG CTAAGACTGG CTCCTGTGCT GCTTGGGAAC 
CCACAGCCGA TGGTCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103946.1
CDS	513..5993
Translation	MQLTTMKSSMWLGTWLSGAKGVKARHWQCTKRSQWTALSSRHCGVTSLYHRISLKRSIVANVWATKYGLLFERCSSSHEVPPSLPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKPEEENAVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFNDSFLAPETEPIVPELCIDHLWTETLPNIREKNSQASKVFITTDLCGQKFLCFLVEAQLQLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLVLEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQAIKFILPKEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSFDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHRNVESHLLNKSLCLTALEVSNAKDEDFSQNLSLDSSTLLFAHIPAIFFVLHLVYEELKLNTLMGEGICSLIDLLVQLARDLKLDSYLDHYYRDSPTLVKTTGQVCTIDQGQMGFMHHPPFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYTLGDESCVSDETCQYLSKVTSTPQKPQAEQEENRFTFRHSASVSVLAERLVVWMASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFSCLHKFAKDFMNYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALIEVTYKGTQWYEQTKEELMAPTLLPELHLLKQMKVKGPRYWELLIDLSKGEQHLRSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISSFTSDPALLSFAEYFCKPTVSMGPKQEILDLFSSILYECVAQETPEMLPAYIAMDQALRSLKKRDMSDTSDLWQIKLILEFFSSRSHQDRQHTYPKRGLFINSEFLPVVKCTVDATLDQWLQAGGDVCVHAYLSGQPVEKSQLNMLACFLVYHSVPAPRHLPPMGLEGSTSFAELLYRFRHLKMPVRALLRLAPVLLGNPQPMVM

Target ORF information:

RefSeq Version	XM_030248086.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus anaphase promoting complex subunit 1 (Anapc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248086.1

ORF Insert Sequence:

ATGCAGCTGA CTACGATGAA GAGCTCTATG TGGCTGGGAA CATGGTTATC TGGAGCAAAG 
GGAGTAAAAG CCAGGCACTG GCAGTGTACA AAGCGTTCAC AGTGGACAGC ACTGTCCAGC 
AGGCATTGTG GTGTGACTTC ATTATATCAC AGGATAAGTC TGAAAAGATC TATAGTGGCA 
AATGTTTGGG CCACTAAATA TGGCTTGCTG TTTGAACGAT GCAGTTCTTC ACATGAGGTA 
CCTCCAAGTC TACCTAGAGA ACCTTTGCCT ACTATGTTCA GCATGCTGCA CCCACTGGAT 
GAGATAACTC CACTTGTTTG TAAATCCGGA AGTCTTTTTG GCTCATCACG GGTACAGTAT 
GTTGTAGATC CAGCAGTGAA AATTGTGTTT CTGAACATTG ACCCCTCCAT TGTCATGACC 
TATGATGCTG TTCAGAATGT GCATTCTGTG TGGACTCTGC GGAGGGTAAA GCCAGAGGAA 
GAGAATGCTG TTTTAAAGTT CCCTGAGCAG GCAGGGACCC TGCAGAATGC AACTACTAGC 
AGCTCCCTGA CAGCACATCT CAGAAGCCTC TCCAAAGGCG AATCCCCTGT GGCTTCGCCG 
TTCCAGAATT ACTCTTCCAT TCACAGCCAG AGTCGCTCAA CCTCTTCGCC CAGTCTACAC 
TCTCGGTCGC CTTCCATTTC CAACATGGCG GCTCTAAGTC GTGCTCACTC TCCTGCCTTA 
GGTGTGCACT CTTTCTCAGG GGCGCAAAGA TTCAACCTTT CAAGCCACAG TCAGTCACCA 
AAGAGACACA GCATTTCTCA CTCTCCGAGT GGCAGTTTCA ACGACTCATT TTTGGCACCA 
GAAACAGAGC CAATTGTTCC TGAGCTGTGC ATTGATCACT TATGGACTGA GACACTTCCG 
AATATAAGAG AGAAAAATTC TCAAGCCTCC AAGGTATTTA TAACGACTGA CCTGTGTGGA 
CAGAAGTTCT TGTGCTTTTT AGTGGAGGCC CAGCTTCAGT TACGCTGTGT AAAGTTTCAA 
GAGAGTAATG ACAAGACTCA GCTTATCTTT GGCTCTGTCA CCAACATACA TGCAAAAGAT 
GCAGCCCCAG TGGAGAAGAT ACACACCATG CTGGTCCTAG AAGGCAACGG GAACCTGGTG 
CTGTACACAG GAGTGGTGCG GGTGGGGAAA GTCTTTATCC CTGGACTTCC GGCTCCTTCC 
CTGACAATGT CCAACATGAT GCCTCGGCCC AGTACCCCGC TCGACGGTGT TGGTACTCCA 
AAGCCTCTGA GTAAATTGCT TGGATCGATG GATGAGGTGG TTCTGTTGTC TCCAGTTCCA 
GAATTGAGGG ATTCTTCAAA ACTTAATGAT TCTCTCTACA ATGAGGATTG CACATTCCAG 
CAGCTTGGAA CTTACATTCA TTCTGTGCGA GACCCTGTTC ATAACAGAGT CACTCTAGAA 
CTGAGCAATG GCTCCATGGT TCGGATCACT ATCCCTGAAG TGGCCACCTC GGAATTAGTA 
CAAACGTGTC TGCAAGCAAT TAAGTTCATC CTGCCAAAAG AAGTAGCCAT TCAGGTGCTC 
GTCAAGTGGT ACAATGTGCA CAGTGCTCCA GGAGGACCCA GCTGTCACTC AGAGTGGAGT 
TTATTTGTGA TTTGTCTCTT GAACATGATG GGTTATAACA CAGACCGCTT AGCATGGACC 
CGAAGTTTTG ACTTTGAAGG ATCACTTTCC CCAGTCATTG CACCCAAAAA AGCAAGGCCT 
TCTGATACTG GATCTGACGA GGACTGGGAG TACTTACTGA ACTCAGAGTA CCATCGCAAT 
GTTGAGTCTC ATCTTTTGAA CAAATCTCTA TGTTTGACTG CTTTGGAAGT TTCAAATGCA 
AAGGATGAAG ATTTTTCGCA GAACCTCAGT CTGGATTCTT CTACCCTTCT CTTTGCTCAC 
ATTCCTGCAA TTTTCTTTGT TCTTCACCTG GTTTATGAAG AGCTCAAGTT GAATACTCTA 
ATGGGAGAAG GAATTTGTTC TCTTATCGAC CTCCTTGTTC AGTTGGCAAG GGACTTAAAA 
TTGGACTCTT ACTTGGACCA TTACTATCGA GATTCCCCAA CTCTTGTCAA AACCACTGGA 
CAAGTATGCA CCATTGACCA AGGTCAGATG GGATTTATGC ATCATCCCCC ATTTTTTACT 
TCTGAGCCAC CAAGTATTTA TCAGTGGGTG AGTTCATGCC TGAAGGGTGA AGGGATGCCA 
CCCTATCCTT ACCTTCCTGG GATTTGCGAG AGGAGCAGGC TAGTGGTCTT GAGCATTGCC 
CTCTATACCC TTGGTGATGA GAGCTGTGTG TCTGATGAAA CTTGCCAGTA CTTATCCAAA 
GTAACCTCAA CTCCCCAAAA GCCACAAGCA GAGCAAGAAG AAAACAGGTT TACTTTCCGG 
CATTCTGCTT CCGTTTCTGT TCTGGCCGAG AGATTAGTTG TCTGGATGGC CAGTGTAGGA 
TTCACTTTAA GAGATCTGGA GACTCTTCCC TTTGGGATTG CTCTTCCCAT CAGAGATGCG 
ATTTACCACT GTCGGGAGCA GCCTGATTCA GATTGGTCAG AAGCGGTCTG TCTCTTGATT 
GGACGTCAGG ATCTTTCCAA GCAGGCTTGT GAAGGAAATT TACCCAGAGG CAAATCTGTG 
CTCTCATCAG AGGTGTCTTC AGGAACTGAG GCAGAGGAGG AAGATGATGG CATGAATGAC 
TTGAACCATG AGGTTATGTC ATTAATATGG AGTGAAGATT TACGGGTGCA GGATGTGCGA 
AGGCTGCTTC AAAGTGCACA GCCTGTCCGT GTCAATGTGG TGCAGTACCC AGAACTCAGT 
GACCATGAGT TCATTGAAGA AAAGGAAAAC AGACTGCTCC AGTTGTGTCA GCGAACTATG 
GCCCTTCCAG TAGGACGAGG AATGTTTACC TTGTTCTCAT ATCATCCTGT TCCAACAGAG 
CCGTTGCCTG TTCCTAAATT GAATCTGACA GGGCGAGCCC CTCCACGAAA CACAACTGTA 
GATCTTAATA GCGGAAACAT CGACGTGCCT CCCAACATGG CCAGCTGGGC CAGCTTTCAT 
AATGGTGTGG CTGCCGGCCT GAAGATAGCC CCAGCCTCCC AGATAGACTC AGCTTGGATC 
GTTTACAACA AGCCAAAGCA TGCTGAGTTA GCCAATGAGT ATGCCGGCTT TCTCATGGCC 
CTGGGTCTGA ATGGGCACCT TACCAAGCTG GCTACTCTCA ATATCCATGA CTATTTGACC 
AAGGGCCATG AAATGACAAG CATTGGATTG CTACTTGGTG TTTCTGCTGC AAAGCTTGGC 
ACCATGGACA TGTCAATTAC CCGGCTCCTC AGCATTCACG TTCCTGCTCT CTTACCCCCG 
ACATCCACAG AGCTTGACGT GCCTCACAAT GTCCAAGTGG CGGCTGTGGT TGGCATTGGC 
CTTGTGTATC AGGGCACAGC TCACAGACAC ACTGCGGAAG TCCTGTTGGC TGAAATAGGG 
CGGCCCCCTG GTCCAGAAAT GGAATACTGC ACTGACAGAG AGTCGTACTC CTTAGCTGCT 
GGCCTGGCCC TGGGCATGGT TTGCTTGGGG CATGGCAGCA ATTTGATTGG CATGTCTGAT 
CTCAATGTGC CTGAGCAGCT GTATCAGTAC ATGGTTGGAG GCCATAGGCG ATTTCAAACT 
GGAATGCACA GGGAGAAACA TAAGTCTCCA AGTTATCAGA TCAAAGAAGG AGACACCATA 
AACGTGGATG TGACTTGTCC CGGTGCTACT CTGGCGTTGG CTATGATCTA CTTAAAAACC 
AATAACAGGT CCATTGCTGA TTGGCTGCGT GCTCCTGATA CCATGTATTT GCTAGACTTT 
GTGAAACCAG AATTCCTTTT GCTTAGGACA CTTGCTCGGT GCTTGATTTT GTGGGATGAT 
ATCTTACCAA ATTCCAAGTG GGTTGACAGC AATGTTCCTC AGATTATAAG AGAAAATAGT 
ATCTCTCTGA GTGAAATTGA ATTGCCTTGT TCAGAGGACT TGAATTTGGA AACCTTGTCG 
CAAGCACACG TCTACATCAT TGCAGGAGCC TGCTTGTCTC TAGGGTTTCG ATTTGCTGGC 
TCAGAAAACT TATCAGCATT TAGCTGTCTG CATAAATTTG CAAAAGATTT TATGAATTAT 
TTATCTGCAC CCAATGCTTC TGTAACAGGG CCCTATAACC TCGAAACCTG CCTGAGTGTG 
GTCCTGCTGT CTCTTGCCAT GGTGATGGCT GGCTCTGGGA ACCTGAAGGT GTTGCAGCTC 
TGTCGCTTTC TGCACATGAA GACTGGTGGA GAGATGAACT ATGGCTTCCA CTTGGCCCAC 
CACATGGCCC TGGGCCTTCT CTTTTTGGGA GGAGGAAGGT ACTCTTTGAG CACATCCAAC 
TCATCCATTG CTGCCCTTCT CTGCGCCCTT TACCCACATT TCCCAGCCCA CAGCACTGAC 
AACCGGTATC ATCTCCAGGC CCTTCGGCAC CTCTATGTGC TAGCTGCAGA ACCAAGGCTC 
CTGGTACCTG TGGATGTGGA TACAAACACA CCCTGCTATG CCCTTATAGA AGTTACTTAC 
AAGGGCACTC AGTGGTATGA ACAGACCAAA GAAGAACTGA TGGCTCCAAC CCTTCTTCCA 
GAACTTCATC TTTTAAAGCA GATGAAAGTT AAAGGGCCAA GATACTGGGA ACTGCTCATA 
GATTTAAGCA AGGGAGAACA GCACTTGAGG TCTATTCTTT CGAAGGATGG AGTTTTATAT 
GTAAAGCTCA GGGCAGGACA GCTCTCCTAC AAAGAAGACC CAATGGGGTG GCAGAGTTTG 
TTGGCACAAA CTGTTGCTAA TAGGAACTCT GAAGCCCGGG CTTTCAAGCC TGAAACAATT 
TCATCATTCA CTTCTGATCC AGCACTTCTG TCATTTGCTG AATATTTCTG CAAGCCGACT 
GTGAGCATGG GTCCAAAACA GGAGATTCTG GACCTCTTTT CTTCGATACT TTATGAGTGT 
GTTGCTCAGG AAACCCCAGA GATGTTGCCT GCCTACATAG CAATGGATCA GGCTCTAAGA 
AGTCTTAAAA AGAGAGACAT GTCAGACACA TCTGATCTTT GGCAGATAAA GTTGATATTA 
GAATTTTTCA GTTCCCGAAG CCACCAGGAT AGGCAGCATA CCTATCCTAA GCGAGGGCTC 
TTCATAAATT CTGAGTTCCT ACCTGTGGTG AAGTGCACTG TTGATGCTAC CCTGGACCAG 
TGGCTACAAG CTGGCGGTGA TGTGTGTGTG CATGCCTACC TCAGTGGGCA GCCTGTTGAG 
AAGTCTCAGC TAAACATGCT GGCCTGCTTC CTTGTCTACC ACTCTGTGCC AGCACCACGA 
CACTTGCCCC CCATGGGACT GGAAGGGAGC ACAAGCTTTG CTGAACTCCT CTACAGGTTC 
CGACACCTGA AGATGCCCGT CCGGGCTTTG CTAAGACTGG CTCCTGTGCT GCTTGGGAAC 
CCACAGCCGA TGGTCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Anapc1 ( NM_008569.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032595.2 Mus musculus anaphase promoting complex subunit 1 (Anapc1), mRNA.

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CloneID	OMu13168
Clone ID Related Accession (Same CDS sequence)	NM_008569.2
Accession Version	NM_008569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	anaphase-promoting complex subunit 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077847.1, BC139002.1, AK034858.1, BE987509.1 and BB676785.1. On Apr 29, 2009 this sequence version replaced NM_008569.1. ##Evidence-Data-START## Transcript exon combination :: AK090134.1, X80169.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAACT TCTCTGAAGA AAGGGCAACG ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTTG GCCGAGACCA CTGCAAGCAC CACCCTAACG CTCTGAACCT TCAGCTTCGT 
CAGCTGCAGC CAGCCTCTGA GTTATGGTCT TCTGATGGTG CAGCTGGCTT GGTGGGATCT 
CTTCAGGAGG TTACAATCCA TGAGAAACAG AAGGAAAGCT GGCAGTTAAG AAAAGGTGTG 
AGTGAGATTG GAGATGCAGC TGACTACGAT GAAGAGCTCT ATGTGGCTGG GAACATGGTT 
ATCTGGAGCA AAGGGAGTAA AAGCCAGGCA CTGGCAGTGT ACAAAGCGTT CACAGTGGAC 
AGCACTGTCC AGCAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAG 
ATCTATAAGA GCCATGAACT AGAGAAATGC ATATGTATAT TACAGAGTTC ATGTATGAAC 
ATGCATAGCA TAGACGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTT 
TGGGCCACTA AATATGGCTT GCTGTTTGAA CGATGCAGTT CTTCACATGA GGTACCTCCA 
AGTCTACCTA GAGAACCTTT GCCTACTATG TTCAGCATGC TGCACCCACT GGATGAGATA 
ACTCCACTTG TTTGTAAATC CGGAAGTCTT TTTGGCTCAT CACGGGTACA GTATGTTGTA 
GATCCAGCAG TGAAAATTGT GTTTCTGAAC ATTGACCCCT CCATTGTCAT GACCTATGAT 
GCTGTTCAGA ATGTGCATTC TGTGTGGACT CTGCGGAGGG TAAAGCCAGA GGAAGAGAAT 
GCTGTTTTAA AGTTCCCTGA GCAGGCAGGG ACCCTGCAGA ATGCAACTAC TAGCAGCTCC 
CTGACAGCAC ATCTCAGAAG CCTCTCCAAA GGCGAATCCC CTGTGGCTTC GCCGTTCCAG 
AATTACTCTT CCATTCACAG CCAGAGTCGC TCAACCTCTT CGCCCAGTCT ACACTCTCGG 
TCGCCTTCCA TTTCCAACAT GGCGGCTCTA AGTCGTGCTC ACTCTCCTGC CTTAGGTGTG 
CACTCTTTCT CAGGGGCGCA AAGATTCAAC CTTTCAAGCC ACAGTCAGTC ACCAAAGAGA 
CACAGCATTT CTCACTCTCC GAGTGGCAGT TTCAACGACT CATTTTTGGC ACCAGAAACA 
GAGCCAATTG TTCCTGAGCT GTGCATTGAT CACTTATGGA CTGAGACACT TCCGAATATA 
AGAGAGAAAA ATTCTCAAGC CTCCAAGGTA TTTATAACGA CTGACCTGTG TGGACAGAAG 
TTCTTGTGCT TTTTAGTGGA GGCCCAGCTT CAGTTACGCT GTGTAAAGTT TCAAGAGAGT 
AATGACAAGA CTCAGCTTAT CTTTGGCTCT GTCACCAACA TACATGCAAA AGATGCAGCC 
CCAGTGGAGA AGATACACAC CATGCTGGTC CTAGAAGGCA ACGGGAACCT GGTGCTGTAC 
ACAGGAGTGG TGCGGGTGGG GAAAGTCTTT ATCCCTGGAC TTCCGGCTCC TTCCCTGACA 
ATGTCCAACA TGATGCCTCG GCCCAGTACC CCGCTCGACG GTGTTGGTAC TCCAAAGCCT 
CTGAGTAAAT TGCTTGGATC GATGGATGAG GTGGTTCTGT TGTCTCCAGT TCCAGAATTG 
AGGGATTCTT CAAAACTTAA TGATTCTCTC TACAATGAGG ATTGCACATT CCAGCAGCTT 
GGAACTTACA TTCATTCTGT GCGAGACCCT GTTCATAACA GAGTCACTCT AGAACTGAGC 
AATGGCTCCA TGGTTCGGAT CACTATCCCT GAAGTGGCCA CCTCGGAATT AGTACAAACG 
TGTCTGCAAG CAATTAAGTT CATCCTGCCA AAAGAAGTAG CCATTCAGGT GCTCGTCAAG 
TGGTACAATG TGCACAGTGC TCCAGGAGGA CCCAGCTGTC ACTCAGAGTG GAGTTTATTT 
GTGATTTGTC TCTTGAACAT GATGGGTTAT AACACAGACC GCTTAGCATG GACCCGAAGT 
TTTGACTTTG AAGGATCACT TTCCCCAGTC ATTGCACCCA AAAAAGCAAG GCCTTCTGAT 
ACTGGATCTG ACGAGGACTG GGAGTACTTA CTGAACTCAG AGTACCATCG CAATGTTGAG 
TCTCATCTTT TGAACAAATC TCTATGTTTG ACTGCTTTGG AAGTTTCAAA TGCAAAGGAT 
GAAGATTTTT CGCAGAACCT CAGTCTGGAT TCTTCTACCC TTCTCTTTGC TCACATTCCT 
GCAATTTTCT TTGTTCTTCA CCTGGTTTAT GAAGAGCTCA AGTTGAATAC TCTAATGGGA 
GAAGGAATTT GTTCTCTTAT CGACCTCCTT GTTCAGTTGG CAAGGGACTT AAAATTGGAC 
TCTTACTTGG ACCATTACTA TCGAGATTCC CCAACTCTTG TCAAAACCAC TGGACAAGTA 
TGCACCATTG ACCAAGGTCA GATGGGATTT ATGCATCATC CCCCATTTTT TACTTCTGAG 
CCACCAAGTA TTTATCAGTG GGTGAGTTCA TGCCTGAAGG GTGAAGGGAT GCCACCCTAT 
CCTTACCTTC CTGGGATTTG CGAGAGGAGC AGGCTAGTGG TCTTGAGCAT TGCCCTCTAT 
ACCCTTGGTG ATGAGAGCTG TGTGTCTGAT GAAACTTGCC AGTACTTATC CAAAGTAACC 
TCAACTCCCC AAAAGCCACA AGCAGAGCAA GAAGAAAACA GGTTTACTTT CCGGCATTCT 
GCTTCCGTTT CTGTTCTGGC CGAGAGATTA GTTGTCTGGA TGGCCAGTGT AGGATTCACT 
TTAAGAGATC TGGAGACTCT TCCCTTTGGG ATTGCTCTTC CCATCAGAGA TGCGATTTAC 
CACTGTCGGG AGCAGCCTGA TTCAGATTGG TCAGAAGCGG TCTGTCTCTT GATTGGACGT 
CAGGATCTTT CCAAGCAGGC TTGTGAAGGA AATTTACCCA GAGGCAAATC TGTGCTCTCA 
TCAGAGGTGT CTTCAGGAAC TGAGGCAGAG GAGGAAGATG ATGGCATGAA TGACTTGAAC 
CATGAGGTTA TGTCATTAAT ATGGAGTGAA GATTTACGGG TGCAGGATGT GCGAAGGCTG 
CTTCAAAGTG CACAGCCTGT CCGTGTCAAT GTGGTGCAGT ACCCAGAACT CAGTGACCAT 
GAGTTCATTG AAGAAAAGGA AAACAGACTG CTCCAGTTGT GTCAGCGAAC TATGGCCCTT 
CCAGTAGGAC GAGGAATGTT TACCTTGTTC TCATATCATC CTGTTCCAAC AGAGCCGTTG 
CCTGTTCCTA AATTGAATCT GACAGGGCGA GCCCCTCCAC GAAACACAAC TGTAGATCTT 
AATAGCGGAA ACATCGACGT GCCTCCCAAC ATGGCCAGCT GGGCCAGCTT TCATAATGGT 
GTGGCTGCCG GCCTGAAGAT AGCCCCAGCC TCCCAGATAG ACTCAGCTTG GATCGTTTAC 
AACAAGCCAA AGCATGCTGA GTTAGCCAAT GAGTATGCCG GCTTTCTCAT GGCCCTGGGT 
CTGAATGGGC ACCTTACCAA GCTGGCTACT CTCAATATCC ATGACTATTT GACCAAGGGC 
CATGAAATGA CAAGCATTGG ATTGCTACTT GGTGTTTCTG CTGCAAAGCT TGGCACCATG 
GACATGTCAA TTACCCGGCT CCTCAGCATT CACGTTCCTG CTCTCTTACC CCCGACATCC 
ACAGAGCTTG ACGTGCCTCA CAATGTCCAA GTGGCGGCTG TGGTTGGCAT TGGCCTTGTG 
TATCAGGGCA CAGCTCACAG ACACACTGCG GAAGTCCTGT TGGCTGAAAT AGGGCGGCCC 
CCTGGTCCAG AAATGGAATA CTGCACTGAC AGAGAGTCGT ACTCCTTAGC TGCTGGCCTG 
GCCCTGGGCA TGGTTTGCTT GGGGCATGGC AGCAATTTGA TTGGCATGTC TGATCTCAAT 
GTGCCTGAGC AGCTGTATCA GTACATGGTT GGAGGCCATA GGCGATTTCA AACTGGAATG 
CACAGGGAGA AACATAAGTC TCCAAGTTAT CAGATCAAAG AAGGAGACAC CATAAACGTG 
GATGTGACTT GTCCCGGTGC TACTCTGGCG TTGGCTATGA TCTACTTAAA AACCAATAAC 
AGGTCCATTG CTGATTGGCT GCGTGCTCCT GATACCATGT ATTTGCTAGA CTTTGTGAAA 
CCAGAATTCC TTTTGCTTAG GACACTTGCT CGGTGCTTGA TTTTGTGGGA TGATATCTTA 
CCAAATTCCA AGTGGGTTGA CAGCAATGTT CCTCAGATTA TAAGAGAAAA TAGTATCTCT 
CTGAGTGAAA TTGAATTGCC TTGTTCAGAG GACTTGAATT TGGAAACCTT GTCGCAAGCA 
CACGTCTACA TCATTGCAGG AGCCTGCTTG TCTCTAGGGT TTCGATTTGC TGGCTCAGAA 
AACTTATCAG CATTTAGCTG TCTGCATAAA TTTGCAAAAG ATTTTATGAA TTATTTATCT 
GCACCCAATG CTTCTGTAAC AGGGCCCTAT AACCTCGAAA CCTGCCTGAG TGTGGTCCTG 
CTGTCTCTTG CCATGGTGAT GGCTGGCTCT GGGAACCTGA AGGTGTTGCA GCTCTGTCGC 
TTTCTGCACA TGAAGACTGG TGGAGAGATG AACTATGGCT TCCACTTGGC CCACCACATG 
GCCCTGGGCC TTCTCTTTTT GGGAGGAGGA AGGTACTCTT TGAGCACATC CAACTCATCC 
ATTGCTGCCC TTCTCTGCGC CCTTTACCCA CATTTCCCAG CCCACAGCAC TGACAACCGG 
TATCATCTCC AGGCCCTTCG GCACCTCTAT GTGCTAGCTG CAGAACCAAG GCTCCTGGTA 
CCTGTGGATG TGGATACAAA CACACCCTGC TATGCCCTTA TAGAAGTTAC TTACAAGGGC 
ACTCAGTGGT ATGAACAGAC CAAAGAAGAA CTGATGGCTC CAACCCTTCT TCCAGAACTT 
CATCTTTTAA AGCAGATGAA AGTTAAAGGG CCAAGATACT GGGAACTGCT CATAGATTTA 
AGCAAGGGAG AACAGCACTT GAGGTCTATT CTTTCGAAGG ATGGAGTTTT ATATGTAAAG 
CTCAGGGCAG GACAGCTCTC CTACAAAGAA GACCCAATGG GGTGGCAGAG TTTGTTGGCA 
CAAACTGTTG CTAATAGGAA CTCTGAAGCC CGGGCTTTCA AGCCTGAAAC AATTTCATCA 
TTCACTTCTG ATCCAGCACT TCTGTCATTT GCTGAATATT TCTGCAAGCC GACTGTGAGC 
ATGGGTCCAA AACAGGAGAT TCTGGACCTC TTTTCTTCGA TACTTTATGA GTGTGTTGCT 
CAGGAAACCC CAGAGATGTT GCCTGCCTAC ATAGCAATGG ATCAGGCTCT AAGAAGTCTT 
AAAAAGAGAG ACATGTCAGA CACATCTGAT CTTTGGCAGA TAAAGTTGAT ATTAGAATTT 
TTCAGTTCCC GAAGCCACCA GGATAGGCAG CATACCTATC CTAAGCGAGG GCTCTTCATA 
AATTCTGAGT TCCTACCTGT GGTGAAGTGC ACTGTTGATG CTACCCTGGA CCAGTGGCTA 
CAAGCTGGCG GTGATGTGTG TGTGCATGCC TACCTCAGTG GGCAGCCTGT TGAGAAGTCT 
CAGCTAAACA TGCTGGCCTG CTTCCTTGTC TACCACTCTG TGCCAGCACC ACGACACTTG 
CCCCCCATGG GACTGGAAGG GAGCACAAGC TTTGCTGAAC TCCTCTACAG GTTCCGACAC 
CTGAAGATGC CCGTCCGGGC TTTGCTAAGA CTGGCTCCTG TGCTGCTTGG GAACCCACAG 
CCGATGGTCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032595.2
CDS	268..6102
Translation	MSNFSEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGDAADYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSTVQQALWCDFIISQDKSEKIYKSHELEKCICILQSSCMNMHSIDGKDYIASLPFQVANVWATKYGLLFERCSSSHEVPPSLPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKPEEENAVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFNDSFLAPETEPIVPELCIDHLWTETLPNIREKNSQASKVFITTDLCGQKFLCFLVEAQLQLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLVLEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPELRDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQAIKFILPKEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSFDFEGSLSPVIAPKKARPSDTGSDEDWEYLLNSEYHRNVESHLLNKSLCLTALEVSNAKDEDFSQNLSLDSSTLLFAHIPAIFFVLHLVYEELKLNTLMGEGICSLIDLLVQLARDLKLDSYLDHYYRDSPTLVKTTGQVCTIDQGQMGFMHHPPFFTSEPPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYTLGDESCVSDETCQYLSKVTSTPQKPQAEQEENRFTFRHSASVSVLAERLVVWMASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGRQDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDLNSGNIDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLVYQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFSCLHKFAKDFMNYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALIEVTYKGTQWYEQTKEELMAPTLLPELHLLKQMKVKGPRYWELLIDLSKGEQHLRSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISSFTSDPALLSFAEYFCKPTVSMGPKQEILDLFSSILYECVAQETPEMLPAYIAMDQALRSLKKRDMSDTSDLWQIKLILEFFSSRSHQDRQHTYPKRGLFINSEFLPVVKCTVDATLDQWLQAGGDVCVHAYLSGQPVEKSQLNMLACFLVYHSVPAPRHLPPMGLEGSTSFAELLYRFRHLKMPVRALLRLAPVLLGNPQPMVM

Target ORF information:

RefSeq Version	NM_008569.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus anaphase promoting complex subunit 1 (Anapc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008569.2

ORF Insert Sequence:

ATGTCGAACT TCTCTGAAGA AAGGGCAACG ATGATTGCAG CCGGGGATTT GCAGGAATTT 
GTTCCTTTTG GCCGAGACCA CTGCAAGCAC CACCCTAACG CTCTGAACCT TCAGCTTCGT 
CAGCTGCAGC CAGCCTCTGA GTTATGGTCT TCTGATGGTG CAGCTGGCTT GGTGGGATCT 
CTTCAGGAGG TTACAATCCA TGAGAAACAG AAGGAAAGCT GGCAGTTAAG AAAAGGTGTG 
AGTGAGATTG GAGATGCAGC TGACTACGAT GAAGAGCTCT ATGTGGCTGG GAACATGGTT 
ATCTGGAGCA AAGGGAGTAA AAGCCAGGCA CTGGCAGTGT ACAAAGCGTT CACAGTGGAC 
AGCACTGTCC AGCAGGCATT GTGGTGTGAC TTCATTATAT CACAGGATAA GTCTGAAAAG 
ATCTATAAGA GCCATGAACT AGAGAAATGC ATATGTATAT TACAGAGTTC ATGTATGAAC 
ATGCATAGCA TAGACGGGAA GGATTATATA GCTTCCTTAC CTTTTCAGGT GGCAAATGTT 
TGGGCCACTA AATATGGCTT GCTGTTTGAA CGATGCAGTT CTTCACATGA GGTACCTCCA 
AGTCTACCTA GAGAACCTTT GCCTACTATG TTCAGCATGC TGCACCCACT GGATGAGATA 
ACTCCACTTG TTTGTAAATC CGGAAGTCTT TTTGGCTCAT CACGGGTACA GTATGTTGTA 
GATCCAGCAG TGAAAATTGT GTTTCTGAAC ATTGACCCCT CCATTGTCAT GACCTATGAT 
GCTGTTCAGA ATGTGCATTC TGTGTGGACT CTGCGGAGGG TAAAGCCAGA GGAAGAGAAT 
GCTGTTTTAA AGTTCCCTGA GCAGGCAGGG ACCCTGCAGA ATGCAACTAC TAGCAGCTCC 
CTGACAGCAC ATCTCAGAAG CCTCTCCAAA GGCGAATCCC CTGTGGCTTC GCCGTTCCAG 
AATTACTCTT CCATTCACAG CCAGAGTCGC TCAACCTCTT CGCCCAGTCT ACACTCTCGG 
TCGCCTTCCA TTTCCAACAT GGCGGCTCTA AGTCGTGCTC ACTCTCCTGC CTTAGGTGTG 
CACTCTTTCT CAGGGGCGCA AAGATTCAAC CTTTCAAGCC ACAGTCAGTC ACCAAAGAGA 
CACAGCATTT CTCACTCTCC GAGTGGCAGT TTCAACGACT CATTTTTGGC ACCAGAAACA 
GAGCCAATTG TTCCTGAGCT GTGCATTGAT CACTTATGGA CTGAGACACT TCCGAATATA 
AGAGAGAAAA ATTCTCAAGC CTCCAAGGTA TTTATAACGA CTGACCTGTG TGGACAGAAG 
TTCTTGTGCT TTTTAGTGGA GGCCCAGCTT CAGTTACGCT GTGTAAAGTT TCAAGAGAGT 
AATGACAAGA CTCAGCTTAT CTTTGGCTCT GTCACCAACA TACATGCAAA AGATGCAGCC 
CCAGTGGAGA AGATACACAC CATGCTGGTC CTAGAAGGCA ACGGGAACCT GGTGCTGTAC 
ACAGGAGTGG TGCGGGTGGG GAAAGTCTTT ATCCCTGGAC TTCCGGCTCC TTCCCTGACA 
ATGTCCAACA TGATGCCTCG GCCCAGTACC CCGCTCGACG GTGTTGGTAC TCCAAAGCCT 
CTGAGTAAAT TGCTTGGATC GATGGATGAG GTGGTTCTGT TGTCTCCAGT TCCAGAATTG 
AGGGATTCTT CAAAACTTAA TGATTCTCTC TACAATGAGG ATTGCACATT CCAGCAGCTT 
GGAACTTACA TTCATTCTGT GCGAGACCCT GTTCATAACA GAGTCACTCT AGAACTGAGC 
AATGGCTCCA TGGTTCGGAT CACTATCCCT GAAGTGGCCA CCTCGGAATT AGTACAAACG 
TGTCTGCAAG CAATTAAGTT CATCCTGCCA AAAGAAGTAG CCATTCAGGT GCTCGTCAAG 
TGGTACAATG TGCACAGTGC TCCAGGAGGA CCCAGCTGTC ACTCAGAGTG GAGTTTATTT 
GTGATTTGTC TCTTGAACAT GATGGGTTAT AACACAGACC GCTTAGCATG GACCCGAAGT 
TTTGACTTTG AAGGATCACT TTCCCCAGTC ATTGCACCCA AAAAAGCAAG GCCTTCTGAT 
ACTGGATCTG ACGAGGACTG GGAGTACTTA CTGAACTCAG AGTACCATCG CAATGTTGAG 
TCTCATCTTT TGAACAAATC TCTATGTTTG ACTGCTTTGG AAGTTTCAAA TGCAAAGGAT 
GAAGATTTTT CGCAGAACCT CAGTCTGGAT TCTTCTACCC TTCTCTTTGC TCACATTCCT 
GCAATTTTCT TTGTTCTTCA CCTGGTTTAT GAAGAGCTCA AGTTGAATAC TCTAATGGGA 
GAAGGAATTT GTTCTCTTAT CGACCTCCTT GTTCAGTTGG CAAGGGACTT AAAATTGGAC 
TCTTACTTGG ACCATTACTA TCGAGATTCC CCAACTCTTG TCAAAACCAC TGGACAAGTA 
TGCACCATTG ACCAAGGTCA GATGGGATTT ATGCATCATC CCCCATTTTT TACTTCTGAG 
CCACCAAGTA TTTATCAGTG GGTGAGTTCA TGCCTGAAGG GTGAAGGGAT GCCACCCTAT 
CCTTACCTTC CTGGGATTTG CGAGAGGAGC AGGCTAGTGG TCTTGAGCAT TGCCCTCTAT 
ACCCTTGGTG ATGAGAGCTG TGTGTCTGAT GAAACTTGCC AGTACTTATC CAAAGTAACC 
TCAACTCCCC AAAAGCCACA AGCAGAGCAA GAAGAAAACA GGTTTACTTT CCGGCATTCT 
GCTTCCGTTT CTGTTCTGGC CGAGAGATTA GTTGTCTGGA TGGCCAGTGT AGGATTCACT 
TTAAGAGATC TGGAGACTCT TCCCTTTGGG ATTGCTCTTC CCATCAGAGA TGCGATTTAC 
CACTGTCGGG AGCAGCCTGA TTCAGATTGG TCAGAAGCGG TCTGTCTCTT GATTGGACGT 
CAGGATCTTT CCAAGCAGGC TTGTGAAGGA AATTTACCCA GAGGCAAATC TGTGCTCTCA 
TCAGAGGTGT CTTCAGGAAC TGAGGCAGAG GAGGAAGATG ATGGCATGAA TGACTTGAAC 
CATGAGGTTA TGTCATTAAT ATGGAGTGAA GATTTACGGG TGCAGGATGT GCGAAGGCTG 
CTTCAAAGTG CACAGCCTGT CCGTGTCAAT GTGGTGCAGT ACCCAGAACT CAGTGACCAT 
GAGTTCATTG AAGAAAAGGA AAACAGACTG CTCCAGTTGT GTCAGCGAAC TATGGCCCTT 
CCAGTAGGAC GAGGAATGTT TACCTTGTTC TCATATCATC CTGTTCCAAC AGAGCCGTTG 
CCTGTTCCTA AATTGAATCT GACAGGGCGA GCCCCTCCAC GAAACACAAC TGTAGATCTT 
AATAGCGGAA ACATCGACGT GCCTCCCAAC ATGGCCAGCT GGGCCAGCTT TCATAATGGT 
GTGGCTGCCG GCCTGAAGAT AGCCCCAGCC TCCCAGATAG ACTCAGCTTG GATCGTTTAC 
AACAAGCCAA AGCATGCTGA GTTAGCCAAT GAGTATGCCG GCTTTCTCAT GGCCCTGGGT 
CTGAATGGGC ACCTTACCAA GCTGGCTACT CTCAATATCC ATGACTATTT GACCAAGGGC 
CATGAAATGA CAAGCATTGG ATTGCTACTT GGTGTTTCTG CTGCAAAGCT TGGCACCATG 
GACATGTCAA TTACCCGGCT CCTCAGCATT CACGTTCCTG CTCTCTTACC CCCGACATCC 
ACAGAGCTTG ACGTGCCTCA CAATGTCCAA GTGGCGGCTG TGGTTGGCAT TGGCCTTGTG 
TATCAGGGCA CAGCTCACAG ACACACTGCG GAAGTCCTGT TGGCTGAAAT AGGGCGGCCC 
CCTGGTCCAG AAATGGAATA CTGCACTGAC AGAGAGTCGT ACTCCTTAGC TGCTGGCCTG 
GCCCTGGGCA TGGTTTGCTT GGGGCATGGC AGCAATTTGA TTGGCATGTC TGATCTCAAT 
GTGCCTGAGC AGCTGTATCA GTACATGGTT GGAGGCCATA GGCGATTTCA AACTGGAATG 
CACAGGGAGA AACATAAGTC TCCAAGTTAT CAGATCAAAG AAGGAGACAC CATAAACGTG 
GATGTGACTT GTCCCGGTGC TACTCTGGCG TTGGCTATGA TCTACTTAAA AACCAATAAC 
AGGTCCATTG CTGATTGGCT GCGTGCTCCT GATACCATGT ATTTGCTAGA CTTTGTGAAA 
CCAGAATTCC TTTTGCTTAG GACACTTGCT CGGTGCTTGA TTTTGTGGGA TGATATCTTA 
CCAAATTCCA AGTGGGTTGA CAGCAATGTT CCTCAGATTA TAAGAGAAAA TAGTATCTCT 
CTGAGTGAAA TTGAATTGCC TTGTTCAGAG GACTTGAATT TGGAAACCTT GTCGCAAGCA 
CACGTCTACA TCATTGCAGG AGCCTGCTTG TCTCTAGGGT TTCGATTTGC TGGCTCAGAA 
AACTTATCAG CATTTAGCTG TCTGCATAAA TTTGCAAAAG ATTTTATGAA TTATTTATCT 
GCACCCAATG CTTCTGTAAC AGGGCCCTAT AACCTCGAAA CCTGCCTGAG TGTGGTCCTG 
CTGTCTCTTG CCATGGTGAT GGCTGGCTCT GGGAACCTGA AGGTGTTGCA GCTCTGTCGC 
TTTCTGCACA TGAAGACTGG TGGAGAGATG AACTATGGCT TCCACTTGGC CCACCACATG 
GCCCTGGGCC TTCTCTTTTT GGGAGGAGGA AGGTACTCTT TGAGCACATC CAACTCATCC 
ATTGCTGCCC TTCTCTGCGC CCTTTACCCA CATTTCCCAG CCCACAGCAC TGACAACCGG 
TATCATCTCC AGGCCCTTCG GCACCTCTAT GTGCTAGCTG CAGAACCAAG GCTCCTGGTA 
CCTGTGGATG TGGATACAAA CACACCCTGC TATGCCCTTA TAGAAGTTAC TTACAAGGGC 
ACTCAGTGGT ATGAACAGAC CAAAGAAGAA CTGATGGCTC CAACCCTTCT TCCAGAACTT 
CATCTTTTAA AGCAGATGAA AGTTAAAGGG CCAAGATACT GGGAACTGCT CATAGATTTA 
AGCAAGGGAG AACAGCACTT GAGGTCTATT CTTTCGAAGG ATGGAGTTTT ATATGTAAAG 
CTCAGGGCAG GACAGCTCTC CTACAAAGAA GACCCAATGG GGTGGCAGAG TTTGTTGGCA 
CAAACTGTTG CTAATAGGAA CTCTGAAGCC CGGGCTTTCA AGCCTGAAAC AATTTCATCA 
TTCACTTCTG ATCCAGCACT TCTGTCATTT GCTGAATATT TCTGCAAGCC GACTGTGAGC 
ATGGGTCCAA AACAGGAGAT TCTGGACCTC TTTTCTTCGA TACTTTATGA GTGTGTTGCT 
CAGGAAACCC CAGAGATGTT GCCTGCCTAC ATAGCAATGG ATCAGGCTCT AAGAAGTCTT 
AAAAAGAGAG ACATGTCAGA CACATCTGAT CTTTGGCAGA TAAAGTTGAT ATTAGAATTT 
TTCAGTTCCC GAAGCCACCA GGATAGGCAG CATACCTATC CTAAGCGAGG GCTCTTCATA 
AATTCTGAGT TCCTACCTGT GGTGAAGTGC ACTGTTGATG CTACCCTGGA CCAGTGGCTA 
CAAGCTGGCG GTGATGTGTG TGTGCATGCC TACCTCAGTG GGCAGCCTGT TGAGAAGTCT 
CAGCTAAACA TGCTGGCCTG CTTCCTTGTC TACCACTCTG TGCCAGCACC ACGACACTTG 
CCCCCCATGG GACTGGAAGG GAGCACAAGC TTTGCTGAAC TCCTCTACAG GTTCCGACAC 
CTGAAGATGC CCGTCCGGGC TTTGCTAAGA CTGGCTCCTG TGCTGCTTGG GAACCCACAG 
CCGATGGTCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Cell reports16(10)2711-2722(2016 09)
Steinmetz CC,Tatavarty V,Sugino K,Shima Y,Joseph A,Lin H,Rutlin M,Lambo M,Hempel CM,Okaty BW,Paradis S,Nelson SB,Turrigiano GG

The E3 ligase APC/C(Cdh1) promotes ubiquitylation-mediated proteolysis of PAX3 to suppress melanocyte proliferation and melanoma growth.
Science signaling8(392)ra87(2015 Sep)
Cao J,Dai X,Wan L,Wang H,Zhang J,Goff PS,Sviderskaya EV,Xuan Z,Xu Z,Xu X,Hinds P,Flaherty KT,Faller DV,Goding CR,Wang Y,Wei W,Cui R

The phosphatase-transcription activator EYA1 is targeted by anaphase-promoting complex/Cdh1 for degradation at M-to-G1 transition.
Molecular and cellular biology33(5)927-36(2013 Mar)
Sun J,Karoulia Z,Wong EY,Ahmed M,Itoh K,Xu PX

Tbx5-hedgehog molecular networks are essential in the second heart field for atrial septation.
Developmental cell23(2)280-91(2012 Aug)
Xie L,Hoffmann AD,Burnicka-Turek O,Friedland-Little JM,Zhang K,Moskowitz IP

Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK.
Genes & development18(3)278-89(2004 Feb)
Fan M,Rhee J,St-Pierre J,Handschin C,Puigserver P,Lin J,J?eger S,Erdjument-Bromage H,Tempst P,Spiegelman BM

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Anapc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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