Mc5r cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mc5r
Entrez Gene ID	17203
Full Name	melanocortin 5 receptor
General protein information	Preferred Names melanocortin 5 receptor Names melanocortin receptor 5 MC5-R
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 E2\|18 41.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013596.2	NP_038624.2	melanocortin receptor 5
NM_013596.3	NP_038624.3	melanocortin receptor 5
XM_030250359.1	XP_030106219.1	melanocortin receptor 5 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MC5R	XP_005615457.1
Mus musculus (house mouse)	Mc5r	NP_038624.2
Pan troglodytes (chimpanzee)	MC5R	NP_001009119.1
Bos taurus (cattle)	MC5R	NP_001015542.1
Rattus norvegicus (Norway rat)	Mc5r	NP_037314.1
Gallus gallus (chicken)	MC5R	NP_001026186.1
Danio rerio (zebrafish)	mc5ra	NP_775386.1
Xenopus tropicalis (tropical clawed frog)	mc5r	NP_001096392.1
Homo sapiens (human)	MC5R	NP_005904.1
	MC5R	XP_001090783.1

Gene Ontology
Bibliography

Related articles in PubMed

MC5r and A2Ar Deficiencies During Experimental Autoimmune Uveitis Identifies Distinct T cell Polarization Programs and a Biphasic Regulatory Response.
Lee DJ, Preble J, Lee S, Foster CS, Taylor AW
Scientific reports637790(2016 Nov)

Biphasic effect of melanocortin agonists on metabolic rate and body temperature.
Lute B, Jou W, Lateef DM, Goldgof M, Xiao C, Pi?ol RA, Kravitz AV, Miller NR, Huang YG, Girardet C, Butler AA, Gavrilova O, Reitman ML
Cell metabolism20(2)333-45(2014 Aug)

Both MC5r and A2Ar are required for protective regulatory immunity in the spleen of post-experimental autoimmune uveitis in mice.
Lee DJ, Taylor AW
Journal of immunology (Baltimore, Md. : 1950)191(8)4103-11(2013 Oct)

Alpha-MSH signalling via melanocortin 5 receptor promotes lipolysis and impairs re-esterification in adipocytes.
Rodrigues AR, Almeida H, Gouveia AM
Biochimica et biophysica acta1831(7)1267-75(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MC5r-dependent regulatory immunity increased CD11b(+)F4/80(+)Ly-6C(low)Ly-6G(+)CD39(+)CD73(+) APCs in the spleen of post-experimental autoimmune uveoretinitis mice.
Title: Both MC5r and A2Ar are required for protective regulatory immunity in the spleen of post-experimental autoimmune uveitis in mice.

In 3T3-L1 adipocytes, alpha-MSH-activated MC5-R signals through the cAMP/PKA and MAPK/ERK1/2 pathways.
Title: Alpha-MSH signalling via melanocortin 5 receptor promotes lipolysis and impairs re-esterification in adipocytes.

Melanocortin receptors 1 and 5 might mediate inhibitory effects of alpha-melanocyte-stimulating hormone on antigen-induced chronic allergic skin inflammation in IgE transgenic mice.
Title: Melanocortin receptors 1 and 5 might mediate inhibitory effects of ?-melanocyte-stimulating hormone on antigen-induced chronic allergic skin inflammation in IgE transgenic mice.

Dependence on MC5r expression promotes or selectively activates IRBP-specific FoxP3+ TGF-beta+ CD25+CD4+ Treg cells in the spleens of experimental uveitis-recovered mice.
Title: Following EAU recovery there is an associated MC5r-dependent APC induction of regulatory immunity in the spleen.

melanocortin 2 receptor and melanocortin 5 receptor are lipolytic mediators in differentiated 3T3-L1 adipocytes
Title: Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved.

The following Mc5r gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mc5r cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09667	NM_013596.2 Latest version!	Mus musculus melanocortin 5 receptor (Mc5r), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu84809	NM_013596.3 Latest version!	Mus musculus melanocortin 5 receptor (Mc5r), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu09667	XM_030250359.1 Latest version!	Mus musculus melanocortin 5 receptor (Mc5r), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mc5r ( NM_013596.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038624.2 Mus musculus melanocortin 5 receptor (Mc5r), mRNA.

Price & Availability↑

CloneID	OMu09667
Clone ID Related Accession (Same CDS sequence)	NM_013596.2 , XM_030250359.1
Accession Version	NM_013596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Mus musculus(house mouse)
Product	melanocortin receptor 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB650258.1 and BC100723.1. On Oct 23, 2007 this sequence version replaced NM_013596.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100720.1, U08354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCAAGACC AGAGCCCGGT AAACAGAAGA TTCAACTCCC AGAAACCGCC TGGAACCCGT 
GAAGAATCAT GCTTACCTTT GCGCGGAGCA GAGCAGAATG GTAAATCCGA TGCCAAGAAG 
TGGGGGCATT CTCTTCCAGC AATGAACTCC TCCTCCACCC TGACTGTATT GAATCTTACC 
CTGAACGCCT CAGAGGATGG CATTTTAGGA TCAAATGTCA AGAACAAGTC TTTGGCCTGT 
GAAGAAATGG GCATTGCCGT GGAGGTGTTC CTGACCCTGG GTCTCGTCAG CCTCTTAGAG 
AACATCCTGG TCATTGGGGC CATAGTAAAG AACAAAAACC TGCACTCACC CATGTACTTC 
TTTGTGGGCA GCTTAGCCGT GGCCGACATG CTGGTGAGCA TGTCCAATGC CTGGGAGACT 
GTCACCATAT ACTTGCTAAA TAATAAACAC CTGGTGATAG CCGACACCTT TGTGCGACAC 
ATCGACAACG TGTTCGACTC CATGATCTGC ATCTCTGTGG TGGCCTCGAT GTGCAGTTTG 
CTGGCCATTG CGGTGGACAG GTACATCACC ATCTTCTATG CCTTGCGCTA CCACCACATC 
ATGACCGCGA GGCGCTCGGG GGTGATCATC GCCTGCATCT GGACCTTCTG CATAAGCTGC 
GGCATTGTTT TCATCATCTA CTATGAGTCC AAGTATGTGA TCATTTGCCT CATCTCCATG 
TTCTTCACCA TGCTGTTCTT CATGGTGTCT CTGTATATAC ACATGTTCCT CCTGGCCCGG 
AACCATGTCA AGCGGATAGC AGCTTCCCCC AGATACAACT CCGTGAGGCA AAGGACCAGC 
ATGAAGGGGG CTATTACCCT CACCATGCTA CTGGGGATTT TCATTGTCTG CTGGTCTCCC 
TTCTTTCTTC ACCTTATCTT AATGATCTCC TGCCCTCAGA ACGTCTACTG CTCTTGCTTT 
ATGTCTTACT TCAACATGTA CCTTATACTC ATCATGTGCA ACTCCGTGAT CGATCCTCTC 
ATCTACGCCC TCCGCAGCCA AGAGATGCGG AGGACCTTTA AGGAGATCGT CTGTTGTCAC 
GGATTCCGGC GACCTTGTAG GCTCCTTGGC GGGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038624.2
CDS	23..1141
Translation	MQDQSPVNRRFNSQKPPGTREESCLPLRGAEQNGKSDAKKWGHSLPAMNSSSTLTVLNLTLNASEDGILGSNVKNKSLACEEMGIAVEVFLTLGLVSLLENILVIGAIVKNKNLHSPMYFFVGSLAVADMLVSMSNAWETVTIYLLNNKHLVIADTFVRHIDNVFDSMICISVVASMCSLLAIAVDRYITIFYALRYHHIMTARRSGVIIACIWTFCISCGIVFIIYYESKYVIICLISMFFTMLFFMVSLYIHMFLLARNHVKRIAASPRYNSVRQRTSMKGAITLTMLLGIFIVCWSPFFLHLILMISCPQNVYCSCFMSYFNMYLILIMCNSVIDPLIYALRSQEMRRTFKEIVCCHGFRRPCRLLGGY

Target ORF information:

RefSeq Version	NM_013596.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanocortin 5 receptor (Mc5r), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013596.2

ORF Insert Sequence:

ATGCAAGACC AGAGCCCGGT AAACAGAAGA TTCAACTCCC AGAAACCGCC TGGAACCCGT 
GAAGAATCAT GCTTACCTTT GCGCGGAGCA GAGCAGAATG GTAAATCCGA TGCCAAGAAG 
TGGGGGCATT CTCTTCCAGC AATGAACTCC TCCTCCACCC TGACTGTATT GAATCTTACC 
CTGAACGCCT CAGAGGATGG CATTTTAGGA TCAAATGTCA AGAACAAGTC TTTGGCCTGT 
GAAGAAATGG GCATTGCCGT GGAGGTGTTC CTGACCCTGG GTCTCGTCAG CCTCTTAGAG 
AACATCCTGG TCATTGGGGC CATAGTAAAG AACAAAAACC TGCACTCACC CATGTACTTC 
TTTGTGGGCA GCTTAGCCGT GGCCGACATG CTGGTGAGCA TGTCCAATGC CTGGGAGACT 
GTCACCATAT ACTTGCTAAA TAATAAACAC CTGGTGATAG CCGACACCTT TGTGCGACAC 
ATCGACAACG TGTTCGACTC CATGATCTGC ATCTCTGTGG TGGCCTCGAT GTGCAGTTTG 
CTGGCCATTG CGGTGGACAG GTACATCACC ATCTTCTATG CCTTGCGCTA CCACCACATC 
ATGACCGCGA GGCGCTCGGG GGTGATCATC GCCTGCATCT GGACCTTCTG CATAAGCTGC 
GGCATTGTTT TCATCATCTA CTATGAGTCC AAGTATGTGA TCATTTGCCT CATCTCCATG 
TTCTTCACCA TGCTGTTCTT CATGGTGTCT CTGTATATAC ACATGTTCCT CCTGGCCCGG 
AACCATGTCA AGCGGATAGC AGCTTCCCCC AGATACAACT CCGTGAGGCA AAGGACCAGC 
ATGAAGGGGG CTATTACCCT CACCATGCTA CTGGGGATTT TCATTGTCTG CTGGTCTCCC 
TTCTTTCTTC ACCTTATCTT AATGATCTCC TGCCCTCAGA ACGTCTACTG CTCTTGCTTT 
ATGTCTTACT TCAACATGTA CCTTATACTC ATCATGTGCA ACTCCGTGAT CGATCCTCTC 
ATCTACGCCC TCCGCAGCCA AGAGATGCGG AGGACCTTTA AGGAGATCGT CTGTTGTCAC 
GGATTCCGGC GACCTTGTAG GCTCCTTGGC GGGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mc5r ( NM_013596.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038624.3 Mus musculus melanocortin 5 receptor (Mc5r), mRNA.

Price & Availability↑

CloneID	OMu84809
Clone ID Related Accession (Same CDS sequence)	NM_013596.3
Accession Version	NM_013596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	melanocortin receptor 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC111069.9. On May 31, 2019 this sequence version replaced NM_013596.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5314783.24371.1, SRR5314783.24371.7 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGAACTCCT CCTCCACCCT GACTGTATTG AATCTTACCC TGAACGCCTC AGAGGATGGC 
ATTTTAGGAT CAAATGTCAA GAACAAGTCT TTGGCCTGTG AAGAAATGGG CATTGCCGTG 
GAGGTGTTCC TGACCCTGGG TCTCGTCAGC CTCTTAGAGA ACATCCTGGT CATTGGGGCC 
ATAGTAAAGA ACAAAAACCT GCACTCACCC ATGTACTTCT TTGTGGGCAG CTTAGCCGTG 
GCCGACATGC TGGTGAGCAT GTCCAATGCC TGGGAGACTG TCACCATATA CTTGCTAAAT 
AATAAACACC TGGTGATAGC CGACACCTTT GTGCGACACA TCGACAACGT GTTCGACTCC 
ATGATCTGCA TCTCTGTGGT GGCCTCGATG TGCAGTTTGC TGGCCATTGC GGTGGACAGG 
TACATCACCA TCTTCTATGC CTTGCGCTAC CACCACATCA TGACCGCGAG GCGCTCGGGG 
GTGATCATCG CCTGCATCTG GACCTTCTGC ATAAGCTGCG GCATTGTTTT CATCATCTAC 
TATGAGTCCA AGTATGTGAT CATTTGCCTC ATCTCCATGT TCTTCACCAT GCTGTTCTTC 
ATGGTGTCTC TGTATATACA CATGTTCCTC CTGGCCCGGA ACCATGTCAA GCGGATAGCA 
GCTTCCCCCA GATACAACTC CGTGAGGCAA AGGACCAGCA TGAAGGGGGC TATTACCCTC 
ACCATGCTAC TGGGGATTTT CATTGTCTGC TGGTCTCCCT TCTTTCTTCA CCTTATCTTA 
ATGATCTCCT GCCCTCAGAA CGTCTACTGC TCTTGCTTTA TGTCTTACTT CAACATGTAC 
CTTATACTCA TCATGTGCAA CTCCGTGATC GATCCTCTCA TCTACGCCCT CCGCAGCCAA 
GAGATGCGGA GGACCTTTAA GGAGATCGTC TGTTGTCACG GATTCCGGCG ACCTTGTAGG 
CTCCTTGGCG GGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038624.3
CDS	256..1233
Translation	MNSSSTLTVLNLTLNASEDGILGSNVKNKSLACEEMGIAVEVFLTLGLVSLLENILVIGAIVKNKNLHSPMYFFVGSLAVADMLVSMSNAWETVTIYLLNNKHLVIADTFVRHIDNVFDSMICISVVASMCSLLAIAVDRYITIFYALRYHHIMTARRSGVIIACIWTFCISCGIVFIIYYESKYVIICLISMFFTMLFFMVSLYIHMFLLARNHVKRIAASPRYNSVRQRTSMKGAITLTMLLGIFIVCWSPFFLHLILMISCPQNVYCSCFMSYFNMYLILIMCNSVIDPLIYALRSQEMRRTFKEIVCCHGFRRPCRLLGGY

Target ORF information:

RefSeq Version	NM_013596.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanocortin 5 receptor (Mc5r), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013596.3

ORF Insert Sequence:

ATGAACTCCT CCTCCACCCT GACTGTATTG AATCTTACCC TGAACGCCTC AGAGGATGGC 
ATTTTAGGAT CAAATGTCAA GAACAAGTCT TTGGCCTGTG AAGAAATGGG CATTGCCGTG 
GAGGTGTTCC TGACCCTGGG TCTCGTCAGC CTCTTAGAGA ACATCCTGGT CATTGGGGCC 
ATAGTAAAGA ACAAAAACCT GCACTCACCC ATGTACTTCT TTGTGGGCAG CTTAGCCGTG 
GCCGACATGC TGGTGAGCAT GTCCAATGCC TGGGAGACTG TCACCATATA CTTGCTAAAT 
AATAAACACC TGGTGATAGC CGACACCTTT GTGCGACACA TCGACAACGT GTTCGACTCC 
ATGATCTGCA TCTCTGTGGT GGCCTCGATG TGCAGTTTGC TGGCCATTGC GGTGGACAGG 
TACATCACCA TCTTCTATGC CTTGCGCTAC CACCACATCA TGACCGCGAG GCGCTCGGGG 
GTGATCATCG CCTGCATCTG GACCTTCTGC ATAAGCTGCG GCATTGTTTT CATCATCTAC 
TATGAGTCCA AGTATGTGAT CATTTGCCTC ATCTCCATGT TCTTCACCAT GCTGTTCTTC 
ATGGTGTCTC TGTATATACA CATGTTCCTC CTGGCCCGGA ACCATGTCAA GCGGATAGCA 
GCTTCCCCCA GATACAACTC CGTGAGGCAA AGGACCAGCA TGAAGGGGGC TATTACCCTC 
ACCATGCTAC TGGGGATTTT CATTGTCTGC TGGTCTCCCT TCTTTCTTCA CCTTATCTTA 
ATGATCTCCT GCCCTCAGAA CGTCTACTGC TCTTGCTTTA TGTCTTACTT CAACATGTAC 
CTTATACTCA TCATGTGCAA CTCCGTGATC GATCCTCTCA TCTACGCCCT CCGCAGCCAA 
GAGATGCGGA GGACCTTTAA GGAGATCGTC TGTTGTCACG GATTCCGGCG ACCTTGTAGG 
CTCCTTGGCG GGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mc5r ( XM_030250359.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106219.1 Mus musculus melanocortin 5 receptor (Mc5r), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09667
Clone ID Related Accession (Same CDS sequence)	NM_013596.2 , XM_030250359.1
Accession Version	XM_030250359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	melanocortin receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGACC AGAGCCCGGT AAACAGAAGA TTCAACTCCC AGAAACCGCC TGGAACCCGT 
GAAGAATCAT GCTTACCTTT GCGCGGAGCA GAGCAGAATG GTAAATCCGA TGCCAAGAAG 
TGGGGGCATT CTCTTCCAGC AATGAACTCC TCCTCCACCC TGACTGTATT GAATCTTACC 
CTGAACGCCT CAGAGGATGG CATTTTAGGA TCAAATGTCA AGAACAAGTC TTTGGCCTGT 
GAAGAAATGG GCATTGCCGT GGAGGTGTTC CTGACCCTGG GTCTCGTCAG CCTCTTAGAG 
AACATCCTGG TCATTGGGGC CATAGTAAAG AACAAAAACC TGCACTCACC CATGTACTTC 
TTTGTGGGCA GCTTAGCCGT GGCCGACATG CTGGTGAGCA TGTCCAATGC CTGGGAGACT 
GTCACCATAT ACTTGCTAAA TAATAAACAC CTGGTGATAG CCGACACCTT TGTGCGACAC 
ATCGACAACG TGTTCGACTC CATGATCTGC ATCTCTGTGG TGGCCTCGAT GTGCAGTTTG 
CTGGCCATTG CGGTGGACAG GTACATCACC ATCTTCTATG CCTTGCGCTA CCACCACATC 
ATGACCGCGA GGCGCTCGGG GGTGATCATC GCCTGCATCT GGACCTTCTG CATAAGCTGC 
GGCATTGTTT TCATCATCTA CTATGAGTCC AAGTATGTGA TCATTTGCCT CATCTCCATG 
TTCTTCACCA TGCTGTTCTT CATGGTGTCT CTGTATATAC ACATGTTCCT CCTGGCCCGG 
AACCATGTCA AGCGGATAGC AGCTTCCCCC AGATACAACT CCGTGAGGCA AAGGACCAGC 
ATGAAGGGGG CTATTACCCT CACCATGCTA CTGGGGATTT TCATTGTCTG CTGGTCTCCC 
TTCTTTCTTC ACCTTATCTT AATGATCTCC TGCCCTCAGA ACGTCTACTG CTCTTGCTTT 
ATGTCTTACT TCAACATGTA CCTTATACTC ATCATGTGCA ACTCCGTGAT CGATCCTCTC 
ATCTACGCCC TCCGCAGCCA AGAGATGCGG AGGACCTTTA AGGAGATCGT CTGTTGTCAC 
GGATTCCGGC GACCTTGTAG GCTCCTTGGC GGGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106219.1
CDS	882..2000
Translation	MQDQSPVNRRFNSQKPPGTREESCLPLRGAEQNGKSDAKKWGHSLPAMNSSSTLTVLNLTLNASEDGILGSNVKNKSLACEEMGIAVEVFLTLGLVSLLENILVIGAIVKNKNLHSPMYFFVGSLAVADMLVSMSNAWETVTIYLLNNKHLVIADTFVRHIDNVFDSMICISVVASMCSLLAIAVDRYITIFYALRYHHIMTARRSGVIIACIWTFCISCGIVFIIYYESKYVIICLISMFFTMLFFMVSLYIHMFLLARNHVKRIAASPRYNSVRQRTSMKGAITLTMLLGIFIVCWSPFFLHLILMISCPQNVYCSCFMSYFNMYLILIMCNSVIDPLIYALRSQEMRRTFKEIVCCHGFRRPCRLLGGY

Target ORF information:

RefSeq Version	XM_030250359.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanocortin 5 receptor (Mc5r), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250359.1

ORF Insert Sequence:

ATGCAAGACC AGAGCCCGGT AAACAGAAGA TTCAACTCCC AGAAACCGCC TGGAACCCGT 
GAAGAATCAT GCTTACCTTT GCGCGGAGCA GAGCAGAATG GTAAATCCGA TGCCAAGAAG 
TGGGGGCATT CTCTTCCAGC AATGAACTCC TCCTCCACCC TGACTGTATT GAATCTTACC 
CTGAACGCCT CAGAGGATGG CATTTTAGGA TCAAATGTCA AGAACAAGTC TTTGGCCTGT 
GAAGAAATGG GCATTGCCGT GGAGGTGTTC CTGACCCTGG GTCTCGTCAG CCTCTTAGAG 
AACATCCTGG TCATTGGGGC CATAGTAAAG AACAAAAACC TGCACTCACC CATGTACTTC 
TTTGTGGGCA GCTTAGCCGT GGCCGACATG CTGGTGAGCA TGTCCAATGC CTGGGAGACT 
GTCACCATAT ACTTGCTAAA TAATAAACAC CTGGTGATAG CCGACACCTT TGTGCGACAC 
ATCGACAACG TGTTCGACTC CATGATCTGC ATCTCTGTGG TGGCCTCGAT GTGCAGTTTG 
CTGGCCATTG CGGTGGACAG GTACATCACC ATCTTCTATG CCTTGCGCTA CCACCACATC 
ATGACCGCGA GGCGCTCGGG GGTGATCATC GCCTGCATCT GGACCTTCTG CATAAGCTGC 
GGCATTGTTT TCATCATCTA CTATGAGTCC AAGTATGTGA TCATTTGCCT CATCTCCATG 
TTCTTCACCA TGCTGTTCTT CATGGTGTCT CTGTATATAC ACATGTTCCT CCTGGCCCGG 
AACCATGTCA AGCGGATAGC AGCTTCCCCC AGATACAACT CCGTGAGGCA AAGGACCAGC 
ATGAAGGGGG CTATTACCCT CACCATGCTA CTGGGGATTT TCATTGTCTG CTGGTCTCCC 
TTCTTTCTTC ACCTTATCTT AATGATCTCC TGCCCTCAGA ACGTCTACTG CTCTTGCTTT 
ATGTCTTACT TCAACATGTA CCTTATACTC ATCATGTGCA ACTCCGTGAT CGATCCTCTC 
ATCTACGCCC TCCGCAGCCA AGAGATGCGG AGGACCTTTA AGGAGATCGT CTGTTGTCAC 
GGATTCCGGC GACCTTGTAG GCTCCTTGGC GGGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MC5r and A2Ar Deficiencies During Experimental Autoimmune Uveitis Identifies Distinct T cell Polarization Programs and a Biphasic Regulatory Response.
Scientific reports637790(2016 Nov)
Lee DJ,Preble J,Lee S,Foster CS,Taylor AW

Biphasic effect of melanocortin agonists on metabolic rate and body temperature.
Cell metabolism20(2)333-45(2014 Aug)
Lute B,Jou W,Lateef DM,Goldgof M,Xiao C,Pi?ol RA,Kravitz AV,Miller NR,Huang YG,Girardet C,Butler AA,Gavrilova O,Reitman ML

Both MC5r and A2Ar are required for protective regulatory immunity in the spleen of post-experimental autoimmune uveitis in mice.
Journal of immunology (Baltimore, Md. : 1950)191(8)4103-11(2013 Oct)
Lee DJ,Taylor AW

Alpha-MSH signalling via melanocortin 5 receptor promotes lipolysis and impairs re-esterification in adipocytes.
Biochimica et biophysica acta1831(7)1267-75(2013 Jul)
Rodrigues AR,Almeida H,Gouveia AM