Mc3r cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mc3r
Entrez Gene ID	17201
Full Name	melanocortin 3 receptor
Synonyms	MC3-R
General protein information	Preferred Names melanocortin 3 receptor Names melanocortin receptor 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H3\|2 94.8 cM
Summary	This gene encodes a member of the melanocortin receptor family. Melanocortin receptors are transmembrane G-protein coupled receptors, which respond to small peptide hormones and exhibit diverse functions and tissue type localization. As part of the central nervous melanocortin system, the encoded protein is competitively bound by either melanocyte stimulating hormone or agouti-related protein to regulate energy homeostasis and adaptation to food restriction. Disruption of this gene results in an increased ratio of weight gain to food intake, increased fat mass, and decreased lean mass, without having a large effect on insulin sensitivity or glucose metabolism. [provided by RefSeq, Dec 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008561.3	NP_032587.1	melanocortin receptor 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	MC3R	NP_063941.3
Pan troglodytes (chimpanzee)	MC3R	XP_525362.1
Mus musculus (house mouse)	Mc3r	NP_032587.1
Rattus norvegicus (Norway rat)	Mc3r	NP_001020441.3
Gallus gallus (chicken)	LOC100857164	XP_004947293.1
Danio rerio (zebrafish)	mc3r	NP_851303.1
Xenopus tropicalis (tropical clawed frog)	mc3r	XP_002935436.1
Canis lupus familiaris (dog)	MC3R	NP_001128596.1
	LOC698439	XP_001091108.1
Bos taurus (cattle)	MC3R	XP_002692472.1

Gene Ontology
Bibliography

Related articles in PubMed

Melanocortin-3 receptors expressed in Nkx2.1(+ve) neurons are sufficient for controlling appetitive responses to hypocaloric conditioning.
Girardet C, Mavrikaki MM, Stevens JR, Miller CA, Marks DL, Butler AA
Scientific reports744444(2017 Mar)

Melanocortin-3 receptors in the limbic system mediate feeding-related motivational responses during weight loss.
Mavrikaki M, Girardet C, Kern A, Faruzzi Brantley A, Miller CA, Macarthur H, Marks DL, Butler AA
Molecular metabolism5(7)566-79(2016 Jul)

A mouse model for a partially inactive obesity-associated human MC3R variant.
Lee B, Koo J, Yun Jun J, Gavrilova O, Lee Y, Seo AY, Taylor-Douglas DC, Adler-Wailes DC, Chen F, Gardner R, Koutzoumis D, Sherafat Kazemzadeh R, Roberson RB, Yanovski JA
Nature communications710522(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Replacing murine Mc3r with and double-mutant (C17A+G241A) human MC3R showed greater weight and fat mass, increased energy intake and feeding efficiency.
Title: A mouse model for a partially inactive obesity-associated human MC3R variant.

MC1-mediated effects were reduced, and MC3 anti-inflammatory circuits predominated. Mice bearing a nonfunctional MC1 displayed a transient exacerbation of neutrophil recruitment after global I/R, which diminished by 2 hours.
Title: Both MC1 and MC3 Receptors Provide Protection From Cerebral Ischemia-Reperfusion-Induced Neutrophil Recruitment.

The minimum promoter region required for Mc3r expression has been identified, along with two binding sites for AP-1 and ATF4 and in the 5' upstream-flanking region of Mc3r that are essential for Mc3r expression.
Title: Functional characterization of the mouse melanocortin 3 receptor gene promoter.

Mc3rs are stronger modulators of food anticipatory activity compared to Ghsrs.
Title: Assessing interactions between Ghsr and Mc3r reveals a role for AgRP in the expression of food anticipatory activity in male mice.

3' RACE experiments using hypothalamic RNA indicated the 3' UTR terminates approximately 1286 bases after the translational stop codon, with a previously unknown 787 base splice between consensus splice donor and acceptor sites.
Title: Evaluation of hypothalamic murine and human melanocortin 3 receptor transcript structure.

The following Mc3r gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mc3r cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13338	NM_008561.3 Latest version!	Mus musculus melanocortin 3 receptor (Mc3r), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mc3r ( NM_008561.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032587.1 Mus musculus melanocortin 3 receptor (Mc3r), mRNA.

Price & Availability↑

CloneID	OMu13338
Clone ID Related Accession (Same CDS sequence)	NM_008561.3
Accession Version	NM_008561.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	melanocortin receptor 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK038677.1. On Apr 6, 2007 this sequence version replaced NM_008561.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AK038677.1 [ECO:0000345] ##Evidence-Data-END##

ATGAACTCTT CCTGCTGCCT GTCTTCTGTT TCTCCGATGC TGCCTAACCT CTCTGAGCAC 
CCTGCAGCCC CTCCTGCCAG CAACCGGAGC GGCAGTGGGT TCTGTGAGCA GGTCTTCATC 
AAGCCGGAGG TCTTCCTGGC TCTGGGCATC GTCAGTCTGA TGGAAAACAT CCTGGTGATC 
CTGGCTGTGG TCAGGAATGG CAACCTGCAC TCTCCCATGT ACTTCTTCCT GTGCAGCCTG 
GCTGCAGCCG ACATGCTGGT GAGCCTGTCC AACTCCCTGG AGACCATCAT GATCGCCGTG 
ATCAACAGCG ACTCCCTGAC CTTGGAGGAC CAGTTTATCC AGCACATGGA TAATATCTTC 
GACTCTATGA TTTGCATCTC CCTGGTGGCC TCCATCTGCA ACCTCCTGGC CATTGCCATC 
GACAGGTACG TCACCATCTT CTATGCCCTT CGGTACCACA GCATCATGAC AGTGAGGAAA 
GCCCTCACCT TGATCGGGGT CATCTGGGTC TGCTGCGGCA TCTGCGGCGT GATGTTCATC 
ATCTACTCCG AGAGCAAGAT GGTCATCGTG TGTCTCATCA CCATGTTCTT CGCCATGGTG 
CTCCTCATGG GCACCCTATA TATCCACATG TTCCTCTTCG CCAGGCTCCA CGTCCAGCGC 
ATCGCAGTGC TGCCCCCTGC TGGCGTGGTG GCCCCACAGC AGCACTCCTG CATGAAGGGG 
GCTGTCACCA TCACTATCCT GCTGGGTGTT TTCATCTTCT GCTGGGCGCC TTTCTTCCTC 
CACCTGGTCC TCATCATCAC CTGCCCCACC AATCCCTACT GCATCTGCTA CACGGCCCAT 
TTCAACACCT ACCTGGTTCT CATCATGTGC AACTCCGTCA TCGACCCCCT CATCTACGCC 
TTCCGCAGCC TGGAGCTGCG CAACACGTTC AAGGAGATTC TCTGCGGCTG CAACAGCATG 
AACTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032587.1
CDS	369..1340
Translation	MNSSCCLSSVSPMLPNLSEHPAAPPASNRSGSGFCEQVFIKPEVFLALGIVSLMENILVILAVVRNGNLHSPMYFFLCSLAAADMLVSLSNSLETIMIAVINSDSLTLEDQFIQHMDNIFDSMICISLVASICNLLAIAIDRYVTIFYALRYHSIMTVRKALTLIGVIWVCCGICGVMFIIYSESKMVIVCLITMFFAMVLLMGTLYIHMFLFARLHVQRIAVLPPAGVVAPQQHSCMKGAVTITILLGVFIFCWAPFFLHLVLIITCPTNPYCICYTAHFNTYLVLIMCNSVIDPLIYAFRSLELRNTFKEILCGCNSMNLG

Target ORF information:

RefSeq Version	NM_008561.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanocortin 3 receptor (Mc3r), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008561.3

ORF Insert Sequence:

ATGAACTCTT CCTGCTGCCT GTCTTCTGTT TCTCCGATGC TGCCTAACCT CTCTGAGCAC 
CCTGCAGCCC CTCCTGCCAG CAACCGGAGC GGCAGTGGGT TCTGTGAGCA GGTCTTCATC 
AAGCCGGAGG TCTTCCTGGC TCTGGGCATC GTCAGTCTGA TGGAAAACAT CCTGGTGATC 
CTGGCTGTGG TCAGGAATGG CAACCTGCAC TCTCCCATGT ACTTCTTCCT GTGCAGCCTG 
GCTGCAGCCG ACATGCTGGT GAGCCTGTCC AACTCCCTGG AGACCATCAT GATCGCCGTG 
ATCAACAGCG ACTCCCTGAC CTTGGAGGAC CAGTTTATCC AGCACATGGA TAATATCTTC 
GACTCTATGA TTTGCATCTC CCTGGTGGCC TCCATCTGCA ACCTCCTGGC CATTGCCATC 
GACAGGTACG TCACCATCTT CTATGCCCTT CGGTACCACA GCATCATGAC AGTGAGGAAA 
GCCCTCACCT TGATCGGGGT CATCTGGGTC TGCTGCGGCA TCTGCGGCGT GATGTTCATC 
ATCTACTCCG AGAGCAAGAT GGTCATCGTG TGTCTCATCA CCATGTTCTT CGCCATGGTG 
CTCCTCATGG GCACCCTATA TATCCACATG TTCCTCTTCG CCAGGCTCCA CGTCCAGCGC 
ATCGCAGTGC TGCCCCCTGC TGGCGTGGTG GCCCCACAGC AGCACTCCTG CATGAAGGGG 
GCTGTCACCA TCACTATCCT GCTGGGTGTT TTCATCTTCT GCTGGGCGCC TTTCTTCCTC 
CACCTGGTCC TCATCATCAC CTGCCCCACC AATCCCTACT GCATCTGCTA CACGGCCCAT 
TTCAACACCT ACCTGGTTCT CATCATGTGC AACTCCGTCA TCGACCCCCT CATCTACGCC 
TTCCGCAGCC TGGAGCTGCG CAACACGTTC AAGGAGATTC TCTGCGGCTG CAACAGCATG 
AACTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Melanocortin-3 receptors expressed in Nkx2.1(+ve) neurons are sufficient for controlling appetitive responses to hypocaloric conditioning.
Scientific reports744444(2017 Mar)
Girardet C,Mavrikaki MM,Stevens JR,Miller CA,Marks DL,Butler AA

Melanocortin-3 receptors in the limbic system mediate feeding-related motivational responses during weight loss.
Molecular metabolism5(7)566-79(2016 Jul)
Mavrikaki M,Girardet C,Kern A,Faruzzi Brantley A,Miller CA,Macarthur H,Marks DL,Butler AA

A mouse model for a partially inactive obesity-associated human MC3R variant.
Nature communications710522(2016 Jan)
Lee B,Koo J,Yun Jun J,Gavrilova O,Lee Y,Seo AY,Taylor-Douglas DC,Adler-Wailes DC,Chen F,Gardner R,Koutzoumis D,Sherafat Kazemzadeh R,Roberson RB,Yanovski JA

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Mc3r cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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