Mapkapk5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mapkapk5
Entrez Gene ID	17165
Full Name	MAP kinase-activated protein kinase 5
Synonyms	MK5,PRAK
General protein information	Preferred Names MAP kinase-activated protein kinase 5 Names MAP kinase-activated protein kinase 5 MAPK-activated protein kinase 5 MAPKAP kinase 5 MAPKAPK-5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_010765.2	NP_034895.1	MAP kinase-activated protein kinase 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAPKAPK5	NP_620777.1
Pan troglodytes (chimpanzee)	MAPKAPK5	XP_003313994.2
Mus musculus (house mouse)	Mapkapk5	NP_034895.1
Rattus norvegicus (Norway rat)	Mapkapk5	NP_001157515.1
Gallus gallus (chicken)	MAPKAPK5	NP_001186736.1
	MAPKAPK5	XP_001102910.2
Canis lupus familiaris (dog)	MAPKAPK5	XP_005636302.1
Bos taurus (cattle)	MAPKAPK5	NP_001193787.1
Xenopus tropicalis (tropical clawed frog)	mapkapk5	NP_001006873.1
Danio rerio (zebrafish)	mapkapk5	NP_001002336.1

Gene Ontology
Bibliography

Related articles in PubMed

MK5: A novel regulator of cardiac fibroblast function?
Sahadevan P, Allen BG
IUBMB life69(10)785-794(2017 Oct)

MK5 haplodeficiency attenuates hypertrophy and preserves diastolic function during remodeling induced by chronic pressure overload in the mouse heart.
Nawaito SA, Dingar D, Sahadevan P, Hussein B, Sahmi F, Shi Y, Gillis MA, Gaestel M, Tardif JC, Allen BG
American journal of physiology. Heart and circulatory physiology313(1)H46-H58(2017 Jul)

Comparative Analysis of Two Gene-Targeting Approaches Challenges the Tumor-Suppressive Role of the Protein Kinase MK5/PRAK.
Ronkina N, Johansen C, Bohlmann L, Lafera J, Menon MB, Tiedje C, Laa? K, Turk BE, Iversen L, Kotlyarov A, Gaestel M
PloS one10(8)e0136138(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MK5 haplodeficiency attenuated the progression of hypertrophy, reduced collagen type 1 mRNA, and protected diastolic function in response to chronic pressure overload.
Title: MK5 haplodeficiency attenuates hypertrophy and preserves diastolic function during remodeling induced by chronic pressure overload in the mouse heart.

An alternative targeting strategy of MK5/PRAK (termed MK5/PRAK-Deltaex6) increased neither tumor incidence in the one step skin carcinogenesis model, nor Ras-induced transformation in primary cells.
Title: Comparative Analysis of Two Gene-Targeting Approaches Challenges the Tumor-Suppressive Role of the Protein Kinase MK5/PRAK.

MK5 positively regulates Rag transcription via phosphorylation of Foxo1 in developing B cells.
Title: MK5 activates Rag transcription via Foxo1 in developing B cells.

Results suggest that PRAK may function as a tumor suppressor in multiple types of cancers.
Title: PRAK suppresses oncogenic ras-induced hematopoietic cancer development by antagonizing the JNK pathway.

observations have demonstrated that PRAK is not required for either the rottlerin-mediated anti-chlamydial activity or rottlerin inhibition of sphingolipid trafficking, suggesting rottlerin may achieve its inhibitory role by targeting other host factors
Title: Rottlerin-mediated inhibition of Chlamydia trachomatis growth and uptake of sphingolipids is independent of p38-regulated/activated protein kinase (PRAK).

The following Mapkapk5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mapkapk5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06826	NM_010765.2 Latest version!	Mus musculus MAP kinase-activated protein kinase 5 (Mapkapk5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$279.30 $399.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mapkapk5 ( NM_010765.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034895.1 Mus musculus MAP kinase-activated protein kinase 5 (Mapkapk5), mRNA.

Price & Availability↑

CloneID	OMu06826
Clone ID Related Accession (Same CDS sequence)	NM_010765.2
Accession Version	NM_010765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	MAP kinase-activated protein kinase 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF039840.1 and BY722898.1. On Aug 14, 2009 this sequence version replaced NM_010765.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF039840.1, SRR1660811.8468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGG ACAGCGACAT GGAGAAAGCC ATCAAGGAGA CCTCCATTTT AGAAGAATAT 
AGTATCAATT GGACTCAGAA ACTGGGAGCC GGAATTAGTG GTCCAGTTAG AGTCTGTGTG 
AAGAAATCCA CTCAAGAACG GTTTGCACTG AAAATTCTTC TTGATCGTCC AAAAGCTAGA 
AATGAGGTGC GCCTGCACAT GATGTGTGCC ACACACCCCA ACATAGTTCA GATTATTGAA 
GTGTTTGCTA ACAGTGTACA GTTCCCTCAT GAGTCCAGCC CCAGGGCTCG ACTCTTAATT 
GTAATGGAGA TGATGGAAGG GGGAGAGCTA TTTCACAGAA TCAGCCAGCA CCGGCACTTT 
ACAGAGAAGC AAGCCAGCCA AGTAACAAAG CAGATAGCCC TGGCTCTACA GCACTGTCAC 
TTGCTAAACA TTGCGCACAG AGACCTCAAG CCTGAAAATC TGCTTTTCAA GGATAACTCT 
CTGGACGCCC CTGTGAAATT ATGTGACTTT GGGTTTGCTA AAGTTGACCA AGGTGATTTG 
ATGACACCCC AGTTTACCCC TTACTATGTA GCACCTCAGG TACTGGAAGC GCAGAGACGG 
CACCAGAAGG AGAAGTCTGG CATCATACCT ACCTCGCCAA CACCCTACAC TTACAACAAG 
AGCTGTGACT TGTGGTCCCT AGGGGTGATA ATTTATGTGA TGCTGTGCGG ATATCCTCCT 
TTTTACTCCA AACACCATAG TCGGACTATC CCAAAGGATA TGCGGAAAAA GATCATGACA 
GGAAGTTTCG AGTTCCCAGA AGAAGAGTGG AGCCAGATCT CAGAGATGGC TAAAGATGTT 
GTGAGGAAGC TTCTGAAGGT CAAACCAGAG GAAAGACTCA CAATCGAGGG AGTGTTGGAC 
CATCCCTGGC TCAACTCGAC AGAGGCCCTG GATAATGTGC TACCCTCTGC CCAGCTGATG 
ATGGATAAGG CGGTGGTTGC GGGGATCCAG CAGGCGCACG CCGAGCAGCT GGCAAACATG 
AGGATCCAGG ACCTCAAGGT CAGCCTCAAA CCCCTGCACT CTGTCAACAA CCCCATTCTC 
AGGAAGAGGA AGCTGCTGGG CACCAAGCCA AAGGACGGTA TTTATATACA CGACCATGAG 
AATGGAACTG AGGACTCAAA TGTTGCCTTG GAAAAGCTTC GAGATGTCAT TGCCCAGTGT 
ATCCTCCCCC AGGCTGGTAA AGGAGAGAAT GAAGATGAGA AGCTGAATGA GGTAATGCAG 
GAGGCCTGGA AGTACAACCG CGAATGCAAG CTCCTGAGGG ATGCTCTGCA GAGTTTTAGC 
TGGAATGGCC GTGGATTCAC AGATAAAGTT GACCGATTGA AGCTGGCAGA GGTGGTAAAG 
CAGGTGATCG AAGAGCAGAC CCTTCCCCAC GAGCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034895.1
CDS	693..2114
Translation	MSEDSDMEKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDGIYIHDHENGTEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ

Target ORF information:

RefSeq Version	NM_010765.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAP kinase-activated protein kinase 5 (Mapkapk5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010765.2

ORF Insert Sequence:

ATGTCGGAGG ACAGCGACAT GGAGAAAGCC ATCAAGGAGA CCTCCATTTT AGAAGAATAT 
AGTATCAATT GGACTCAGAA ACTGGGAGCC GGAATTAGTG GTCCAGTTAG AGTCTGTGTG 
AAGAAATCCA CTCAAGAACG GTTTGCACTG AAAATTCTTC TTGATCGTCC AAAAGCTAGA 
AATGAGGTGC GCCTGCACAT GATGTGTGCC ACACACCCCA ACATAGTTCA GATTATTGAA 
GTGTTTGCTA ACAGTGTACA GTTCCCTCAT GAGTCCAGCC CCAGGGCTCG ACTCTTAATT 
GTAATGGAGA TGATGGAAGG GGGAGAGCTA TTTCACAGAA TCAGCCAGCA CCGGCACTTT 
ACAGAGAAGC AAGCCAGCCA AGTAACAAAG CAGATAGCCC TGGCTCTACA GCACTGTCAC 
TTGCTAAACA TTGCGCACAG AGACCTCAAG CCTGAAAATC TGCTTTTCAA GGATAACTCT 
CTGGACGCCC CTGTGAAATT ATGTGACTTT GGGTTTGCTA AAGTTGACCA AGGTGATTTG 
ATGACACCCC AGTTTACCCC TTACTATGTA GCACCTCAGG TACTGGAAGC GCAGAGACGG 
CACCAGAAGG AGAAGTCTGG CATCATACCT ACCTCGCCAA CACCCTACAC TTACAACAAG 
AGCTGTGACT TGTGGTCCCT AGGGGTGATA ATTTATGTGA TGCTGTGCGG ATATCCTCCT 
TTTTACTCCA AACACCATAG TCGGACTATC CCAAAGGATA TGCGGAAAAA GATCATGACA 
GGAAGTTTCG AGTTCCCAGA AGAAGAGTGG AGCCAGATCT CAGAGATGGC TAAAGATGTT 
GTGAGGAAGC TTCTGAAGGT CAAACCAGAG GAAAGACTCA CAATCGAGGG AGTGTTGGAC 
CATCCCTGGC TCAACTCGAC AGAGGCCCTG GATAATGTGC TACCCTCTGC CCAGCTGATG 
ATGGATAAGG CGGTGGTTGC GGGGATCCAG CAGGCGCACG CCGAGCAGCT GGCAAACATG 
AGGATCCAGG ACCTCAAGGT CAGCCTCAAA CCCCTGCACT CTGTCAACAA CCCCATTCTC 
AGGAAGAGGA AGCTGCTGGG CACCAAGCCA AAGGACGGTA TTTATATACA CGACCATGAG 
AATGGAACTG AGGACTCAAA TGTTGCCTTG GAAAAGCTTC GAGATGTCAT TGCCCAGTGT 
ATCCTCCCCC AGGCTGGTAA AGGAGAGAAT GAAGATGAGA AGCTGAATGA GGTAATGCAG 
GAGGCCTGGA AGTACAACCG CGAATGCAAG CTCCTGAGGG ATGCTCTGCA GAGTTTTAGC 
TGGAATGGCC GTGGATTCAC AGATAAAGTT GACCGATTGA AGCTGGCAGA GGTGGTAAAG 
CAGGTGATCG AAGAGCAGAC CCTTCCCCAC GAGCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MK5: A novel regulator of cardiac fibroblast function?
IUBMB life69(10)785-794(2017 Oct)
Sahadevan P,Allen BG

MK5 haplodeficiency attenuates hypertrophy and preserves diastolic function during remodeling induced by chronic pressure overload in the mouse heart.
American journal of physiology. Heart and circulatory physiology313(1)H46-H58(2017 Jul)
Nawaito SA,Dingar D,Sahadevan P,Hussein B,Sahmi F,Shi Y,Gillis MA,Gaestel M,Tardif JC,Allen BG

Comparative Analysis of Two Gene-Targeting Approaches Challenges the Tumor-Suppressive Role of the Protein Kinase MK5/PRAK.
PloS one10(8)e0136138(2015)
Ronkina N,Johansen C,Bohlmann L,Lafera J,Menon MB,Tiedje C,Laa? K,Turk BE,Iversen L,Kotlyarov A,Gaestel M

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Mapkapk5 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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