Man2b1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man2b1
Entrez Gene ID	17159
Full Name	mannosidase 2, alpha B1
Synonyms	AW107687,LAMAN
General protein information	Preferred Names mannosidase 2, alpha B1 Names lysosomal alpha-mannosidase lysosomal acid alpha-mannosidase mannosidase alpha class 2B member 1 mannosidase alpha-B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 C3\|8 41.55 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530747.3	XP_006530810.1	lysosomal alpha-mannosidase isoform X1
XM_006530749.3	XP_006530812.1	lysosomal alpha-mannosidase isoform X2
XM_006530748.3	XP_006530811.1	lysosomal alpha-mannosidase isoform X2
NM_010764.2	NP_034894.2	lysosomal alpha-mannosidase precursor
XM_006530748.2	XP_006530811.1	lysosomal alpha-mannosidase isoform X2
XM_006530747.2	XP_006530810.1	lysosomal alpha-mannosidase isoform X1
XM_006530749.2	XP_006530812.1	lysosomal alpha-mannosidase isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2B1	XP_003316198.2
Arabidopsis thaliana (thale cress)	AT5G13980	NP_196902.2
Bos taurus (cattle)	MAN2B1	NP_776986.2
Rattus norvegicus (Norway rat)	Man2b1	NP_955436.1
Mus musculus (house mouse)	Man2b1	NP_034894.2
Drosophila melanogaster (fruit fly)	LM408	NP_609408.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008584	XP_557731.3
Xenopus tropicalis (tropical clawed frog)	man2b1	NP_001072469.1
Homo sapiens (human)	MAN2B1	NP_000519.2
	LOC716173	XP_002801133.1
Canis lupus familiaris (dog)	MAN2B1	XP_005632890.1
Danio rerio (zebrafish)	man2b1	NP_001014354.1
Caenorhabditis elegans (roundworm)	aman-1	NP_508811.1
Oryza sativa (rice)	Os11g0525600	NP_001067993.1

Gene Ontology
Bibliography

Related articles in PubMed

DLK1 is a novel regulator of bone mass that mediates estrogen deficiency-induced bone loss in mice.
Abdallah BM, Ditzel N, Mahmood A, Isa A, Traustadottir GA, Schilling AF, Ruiz-Hidalgo MJ, Laborda J, Amling M, Kassem M
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research26(7)1457-71(2011 Jul)

Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis.
Damme M, Morelle W, Schmidt B, Andersson C, Fogh J, Michalski JC, L?bke T
Molecular and cellular biology30(1)273-83(2010 Jan)

Liver sinusoidal endothelial cells depend on mannose receptor-mediated recruitment of lysosomal enzymes for normal degradation capacity.
Elvevold K, Simon-Santamaria J, Hasvold H, McCourt P, Smedsr?d B, S?rensen KK
Hepatology (Baltimore, Md.)48(6)2007-15(2008 Dec)

Characterization of the murine Inpp4b gene and identification of a novel isoform.
Ferron M, Vacher J
Gene376(1)152-61(2006 Jul)

Neurocognitive and psychotiform behavioral alterations and enhanced hippocampal long-term potentiation in transgenic mice displaying neuropathological features of human alpha-mannosidosis.
D'Hooge R, L?llmann-Rauch R, Beckers T, Balschun D, Schwake M, Reiss K, von Figura K, Saftig P
The Journal of neuroscience : the official journal of the Society for Neuroscience25(28)6539-49(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mannose receptor (-/-) liver sinusoidal endothelial cells had markedly and significantly reduced enzyme activities for four out of five lysosomal enzymes tested, i.e., cathepsin-D, alpha-mannosidase, beta-hexosaminidase and arylsulfatase.
Title: Liver sinusoidal endothelial cells depend on mannose receptor-mediated recruitment of lysosomal enzymes for normal degradation capacity.

The following Man2b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man2b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu41273	XM_006530749.3 Latest version!	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu41273	XM_006530748.3 Latest version!	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu41272	XM_006530747.3 Latest version!	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu06823	NM_010764.2 Latest version!	Mus musculus mannosidase 2, alpha B1 (Man2b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$594.30 $849.00
View Old Accession Versions >>
OMu41273	XM_006530748.2	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu41272	XM_006530747.2	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu41273	XM_006530749.2	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man2b1 ( XM_006530748.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530811.1 Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu41273
Clone ID Related Accession (Same CDS sequence)	XM_006530749.3 , XM_006530748.3 , XM_006530748.2 , XM_006530749.2
Accession Version	XM_006530748.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530811.1
CDS	859..3540
Translation	MAFFSRWWKQQTSATQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVDNPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQVNGSLVHVLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNFSFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETYQWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENLEQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQVIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTEPVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSYPEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA

Target ORF information:

RefSeq Version	XM_006530748.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530748.2

ORF Insert Sequence:

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_006530749.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530812.1 Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu41273
Clone ID Related Accession (Same CDS sequence)	XM_006530749.3 , XM_006530748.3 , XM_006530748.2 , XM_006530749.2
Accession Version	XM_006530749.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530812.1
CDS	367..3048
Translation	MAFFSRWWKQQTSATQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVDNPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQVNGSLVHVLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNFSFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETYQWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENLEQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQVIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTEPVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSYPEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA

Target ORF information:

RefSeq Version	XM_006530749.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530749.2

ORF Insert Sequence:

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_006530749.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530812.1 Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X2, mRNA.

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CloneID	OMu41273
Clone ID Related Accession (Same CDS sequence)	XM_006530749.3 , XM_006530748.3 , XM_006530748.2 , XM_006530749.2
Accession Version	XM_006530749.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530812.1
CDS	975..3656
Translation	MAFFSRWWKQQTSATQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVDNPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQVNGSLVHVLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNFSFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETYQWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENLEQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQVIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTEPVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSYPEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA

Target ORF information:

RefSeq Version	XM_006530749.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530749.3

ORF Insert Sequence:

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_006530748.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530811.1 Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu41273
Clone ID Related Accession (Same CDS sequence)	XM_006530749.3 , XM_006530748.3 , XM_006530748.2 , XM_006530749.2
Accession Version	XM_006530748.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530748.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530811.1
CDS	858..3539
Translation	MAFFSRWWKQQTSATQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVDNPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQVNGSLVHVLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNFSFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETYQWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENLEQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQVIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTEPVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSYPEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA

Target ORF information:

RefSeq Version	XM_006530748.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530748.3

ORF Insert Sequence:

ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_006530747.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530810.1 Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X1, mRNA.

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CloneID	OMu41272
Clone ID Related Accession (Same CDS sequence)	XM_006530747.3 , XM_006530747.2
Accession Version	XM_006530747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG GACCGCTCAC CTCTGGTGTC CGTGCTGGTG GCGGGAACAC CGGGTGGCTC 
TGGATGAGCT CCTGCAACCT AGGTTCGCCA GTCCTGCCTA TTTCCTTTCT TTTTTGGCTG 
TTGCTGGCCG CGCCTGGCGC TCGGGCCGCG GGTTACAAGA CATGTCCCCC AACGAAGCCA 
GGCATGCTCA ATGTGCACCT GCTGCCTCAT ACACACGATG ATGTAGGCTG GCTGAAGACA 
GTGGACCAAT ACTATTACGG CATGGACCTT GACTTCCTGA ACTTCATGCC CACAGTCCTG 
AGTGATGTTC AGCACGCATC TGTTCAGTAC ATCCTAGACT CAGTAGTCTC CTCTCTACTG 
GAGAAACCCA CCCGTCGCTT CATCTATGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA GTGCAACCCA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT GATGAACGAC GAAGCAGCCA CCCACTATGG CGCGATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAAGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCATATTGA CCCTTTCGGC CACTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTTGGG CGCATTGACT ATCAAGATAA ACTTAATCGG 
AAGAAAAAGC TGAGGATGGA GGAGCTGTGG AGAGCTAGTG ACAGCTTGGA GCCCCCCGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CCCCGAAATA CCTGTGCTGG 
GATGTGCTAT GTACAGACCC TCCCGTGGTG GATAATCCCC GGAGCCCTGA GTTCAATGCA 
AAAACGTTGG TCAACTACTT CCTGAAGCTC GCTTCTTCCC AGAAAGGGTT TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCATTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTGAT CCGCCTGGTC AACGCGCAGC AGGTAAACGG GAGCCTCGTC 
CACGTTCTCT ACTCCACCCC TACGTGTTAC CTCTGGGAGC TGAACAAGGC CAATCTCACC 
TGGACAGTGA AGGAGGACGA CTTCTTTCCT TATGCCGATG GCCCCCACAT GTTCTGGACT 
GGCTATTTTT CCAGCAGGCC AGCCCTCAAG CGCTATGAGC GCCTCAGTTA CAACTTCCTG 
CAGGTGTGTA ACCAGCTGGA AGCACTGGTG GGTCCCGAAG CCAACGTGGG ACCCTATGGC 
TCGGGAGACA GCGCACCTCT TCAGGAGGCA ATGGCGGTGC TCCAACACCA CGACGCTGTC 
AGTGGTACTG CAAGGCAGAA TGTTGTAAAT GACTACGCGA GGCAGCTGGC AGCAGGCTGG 
GGACCCTGTG AGGTTCTCGT GAGCAACGCT CTGGCGCGAC TCAGCCATTA CAAACAGAAC 
TTCTCATTCT GTCGTGAGCT CAACATTAGC ATCTGCCCAG TCAGCCAGAC TTCGGAGCGT 
TTCCAGGTAA CTCTTTACAA CCCCCTGGGG CGAAAGGTGG ATCAGATGGT TCGGCTGCCT 
GTCTATGAAG GCAACTTCAT TGTGAAGGAC CCTCATGATA AGAACATTTC CAGTAACGTG 
GTGATGGTTC CTAGCTATTA CAGTGAGACT TACCAATGGG AGCTGCTGTT CCCAGCCTCA 
GTGCCTGCCC TGGGCTTCAG CACCTACTCT GTGGCCAAAA TGAGTGACCT TAACCACCAA 
GCCCACAACC TGCTGTCCAG GCCCAGGAAA CACAAGTCCC ATCATGTCTT AGTCATTGAA 
AATAAGTACA TGCGGGCCAC ATTTGACTCT GGCACGGGGT TATTGATGAA GATTGAAAAC 
TTGGAACAGA ACCTCTCGCT CCCTGTGAGC CAAGGCTTCT TTTGGTATAA TGCCAGTGTT 
GGTGATGAGG AGAGTTCTCA GGCCTCTGGG GCCTACATCT TCAGACCCAA TGTCGGTAAA 
CCGATACCTG TGAGCCGCTG GGCTCAGATC AGTTTGGTGA AGACAGCCTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTACAA AGGACAGCGC 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA GAGATGACTG GGGGAAGGAA 
GTCATCAGCC GCTTTGACAC CCCAATGAAA ACCAAGGGAC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGATGAT TATCGACCCA CCTGGACATT GAATCAGACT 
GAGCCGGTGG CTGGAAATTA CTATCCTGTC AACACTCGAA TTTATATCAC GGATGGGCAA 
ATGCAGCTGA CCGTGTTGAC CGACCGCTCC CAGGGGGGCA GCAGTCTGCA GGATGGCTCG 
TTGGAGCTCA TGGTGCACCG TCGGCTGTTG GTAGATGATG ATCGTGGAGT GTCGGAACCC 
TTGTTGGAGA CAGATACCGG GGATAAGGTT CGAGGACGCC ACCTTGTGCT TTTGAGTTCT 
GTCAGTGATG CCGCTGCAAG ACACCGACTG CTGGCGGAAC AAGAAGTCCT GGCCCCTCAG 
GTGGTGCTGT CTCTGGGTGG TAGCAGTCCC TATCACTCCC GGGCGACACC AAAAACGCAG 
TTTTCCGGAC TTCGCCAGGA GCTACCTCCT CAGGTGCATT TGCTCACACT GGCCCGCTGG 
GGGCCAAAGA TGCTGCTGCT GCGCTTGGAG CACCAGTTCG CTCTGAAAGA AGATTCGGAT 
CGCAACCTGA GCTCCCCTGT AACCTTGAAC GTTCAGAACC TGTTCCAGAC CTTCACTATC 
AACTACCTGC AGGAGACCAC ACTGGCAGCC AACCAGCCCC TGTCCAGGGC TTCCAGGCTC 
AAGTGGATGA CAAATACTGG TCCCACCTCC TACCCTGAGC CTTCCAAGTT GGACCCTACC 
TCAGTCACAC TGAAGCCTAT GGAAATACGT ACCTTCCTGG CCTCTGTACA ATGGCAAGAA 
CACAGGCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530810.1
CDS	599..3673
Translation	MGTGPLTSGVRAGGGNTGWLWMSSCNLGSPVLPISFLFWLLLAAPGARAAGYKTCPPTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGMDLDFLNFMPTVLSDVQHASVQYILDSVVSSLLEKPTRRFIYVEMAFFSRWWKQQTSATQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVDNPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQVNGSLVHVLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNFSFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETYQWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENLEQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQVIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTEPVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSYPEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA

Target ORF information:

RefSeq Version	XM_006530747.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530747.3

ORF Insert Sequence:

ATGGGCACCG GACCGCTCAC CTCTGGTGTC CGTGCTGGTG GCGGGAACAC CGGGTGGCTC 
TGGATGAGCT CCTGCAACCT AGGTTCGCCA GTCCTGCCTA TTTCCTTTCT TTTTTGGCTG 
TTGCTGGCCG CGCCTGGCGC TCGGGCCGCG GGTTACAAGA CATGTCCCCC AACGAAGCCA 
GGCATGCTCA ATGTGCACCT GCTGCCTCAT ACACACGATG ATGTAGGCTG GCTGAAGACA 
GTGGACCAAT ACTATTACGG CATGGACCTT GACTTCCTGA ACTTCATGCC CACAGTCCTG 
AGTGATGTTC AGCACGCATC TGTTCAGTAC ATCCTAGACT CAGTAGTCTC CTCTCTACTG 
GAGAAACCCA CCCGTCGCTT CATCTATGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA GTGCAACCCA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT GATGAACGAC GAAGCAGCCA CCCACTATGG CGCGATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAAGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCATATTGA CCCTTTCGGC CACTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTTGGG CGCATTGACT ATCAAGATAA ACTTAATCGG 
AAGAAAAAGC TGAGGATGGA GGAGCTGTGG AGAGCTAGTG ACAGCTTGGA GCCCCCCGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CCCCGAAATA CCTGTGCTGG 
GATGTGCTAT GTACAGACCC TCCCGTGGTG GATAATCCCC GGAGCCCTGA GTTCAATGCA 
AAAACGTTGG TCAACTACTT CCTGAAGCTC GCTTCTTCCC AGAAAGGGTT TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCATTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTGAT CCGCCTGGTC AACGCGCAGC AGGTAAACGG GAGCCTCGTC 
CACGTTCTCT ACTCCACCCC TACGTGTTAC CTCTGGGAGC TGAACAAGGC CAATCTCACC 
TGGACAGTGA AGGAGGACGA CTTCTTTCCT TATGCCGATG GCCCCCACAT GTTCTGGACT 
GGCTATTTTT CCAGCAGGCC AGCCCTCAAG CGCTATGAGC GCCTCAGTTA CAACTTCCTG 
CAGGTGTGTA ACCAGCTGGA AGCACTGGTG GGTCCCGAAG CCAACGTGGG ACCCTATGGC 
TCGGGAGACA GCGCACCTCT TCAGGAGGCA ATGGCGGTGC TCCAACACCA CGACGCTGTC 
AGTGGTACTG CAAGGCAGAA TGTTGTAAAT GACTACGCGA GGCAGCTGGC AGCAGGCTGG 
GGACCCTGTG AGGTTCTCGT GAGCAACGCT CTGGCGCGAC TCAGCCATTA CAAACAGAAC 
TTCTCATTCT GTCGTGAGCT CAACATTAGC ATCTGCCCAG TCAGCCAGAC TTCGGAGCGT 
TTCCAGGTAA CTCTTTACAA CCCCCTGGGG CGAAAGGTGG ATCAGATGGT TCGGCTGCCT 
GTCTATGAAG GCAACTTCAT TGTGAAGGAC CCTCATGATA AGAACATTTC CAGTAACGTG 
GTGATGGTTC CTAGCTATTA CAGTGAGACT TACCAATGGG AGCTGCTGTT CCCAGCCTCA 
GTGCCTGCCC TGGGCTTCAG CACCTACTCT GTGGCCAAAA TGAGTGACCT TAACCACCAA 
GCCCACAACC TGCTGTCCAG GCCCAGGAAA CACAAGTCCC ATCATGTCTT AGTCATTGAA 
AATAAGTACA TGCGGGCCAC ATTTGACTCT GGCACGGGGT TATTGATGAA GATTGAAAAC 
TTGGAACAGA ACCTCTCGCT CCCTGTGAGC CAAGGCTTCT TTTGGTATAA TGCCAGTGTT 
GGTGATGAGG AGAGTTCTCA GGCCTCTGGG GCCTACATCT TCAGACCCAA TGTCGGTAAA 
CCGATACCTG TGAGCCGCTG GGCTCAGATC AGTTTGGTGA AGACAGCCTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTACAA AGGACAGCGC 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA GAGATGACTG GGGGAAGGAA 
GTCATCAGCC GCTTTGACAC CCCAATGAAA ACCAAGGGAC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGATGAT TATCGACCCA CCTGGACATT GAATCAGACT 
GAGCCGGTGG CTGGAAATTA CTATCCTGTC AACACTCGAA TTTATATCAC GGATGGGCAA 
ATGCAGCTGA CCGTGTTGAC CGACCGCTCC CAGGGGGGCA GCAGTCTGCA GGATGGCTCG 
TTGGAGCTCA TGGTGCACCG TCGGCTGTTG GTAGATGATG ATCGTGGAGT GTCGGAACCC 
TTGTTGGAGA CAGATACCGG GGATAAGGTT CGAGGACGCC ACCTTGTGCT TTTGAGTTCT 
GTCAGTGATG CCGCTGCAAG ACACCGACTG CTGGCGGAAC AAGAAGTCCT GGCCCCTCAG 
GTGGTGCTGT CTCTGGGTGG TAGCAGTCCC TATCACTCCC GGGCGACACC AAAAACGCAG 
TTTTCCGGAC TTCGCCAGGA GCTACCTCCT CAGGTGCATT TGCTCACACT GGCCCGCTGG 
GGGCCAAAGA TGCTGCTGCT GCGCTTGGAG CACCAGTTCG CTCTGAAAGA AGATTCGGAT 
CGCAACCTGA GCTCCCCTGT AACCTTGAAC GTTCAGAACC TGTTCCAGAC CTTCACTATC 
AACTACCTGC AGGAGACCAC ACTGGCAGCC AACCAGCCCC TGTCCAGGGC TTCCAGGCTC 
AAGTGGATGA CAAATACTGG TCCCACCTCC TACCCTGAGC CTTCCAAGTT GGACCCTACC 
TCAGTCACAC TGAAGCCTAT GGAAATACGT ACCTTCCTGG CCTCTGTACA ATGGCAAGAA 
CACAGGCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( NM_010764.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034894.2 Mus musculus mannosidase 2, alpha B1 (Man2b1), mRNA.

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CloneID	OMu06823
Clone ID Related Accession (Same CDS sequence)	NM_010764.2
Accession Version	NM_010764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-mannosidase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY289778.1, AC163703.4, AK004817.2 and AI480690.1. On Aug 24, 2006 this sequence version replaced NM_010764.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK035551.1, BC005430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCACCG GACCGCTCAC CTCTGGTGTC CGTGCTGGTG GCGGGAACAC CGGGTGGCTC 
TGGATGAGCT CCTGCAACCT AGGTTCGCCA GTCCTGCCTA TTTCCTTTCT TTTTTGGCTG 
TTGCTGGCCG CGCCTGGCGC TCGGGCCGCG GGTTACAAGA CATGTCCCCC AACGAAGCCA 
GGCATGCTCA ATGTGCACCT GCTGCCTCAT ACACACGATG ATGTAGGCTG GCTGAAGACA 
GTGGACCAAT ACTATTACGG CATCCTGAGT GATGTTCAGC ACGCATCTGT TCAGTACATC 
CTAGACTCAG TAGTCTCCTC TCTACTGGAG AAACCCACCC GTCGCTTCAT CTATGTGGAA 
ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034894.2
CDS	62..3103
Translation	MGTGPLTSGVRAGGGNTGWLWMSSCNLGSPVLPISFLFWLLLAAPGARAAGYKTCPPTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGILSDVQHASVQYILDSVVSSLLEKPTRRFIYVEMAFFSRWWKQQTSATQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVDNPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQVNGSLVHVLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNFSFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETYQWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENLEQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQVIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTEPVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSYPEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA

Target ORF information:

RefSeq Version	NM_010764.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha B1 (Man2b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010764.2

ORF Insert Sequence:

ATGGGCACCG GACCGCTCAC CTCTGGTGTC CGTGCTGGTG GCGGGAACAC CGGGTGGCTC 
TGGATGAGCT CCTGCAACCT AGGTTCGCCA GTCCTGCCTA TTTCCTTTCT TTTTTGGCTG 
TTGCTGGCCG CGCCTGGCGC TCGGGCCGCG GGTTACAAGA CATGTCCCCC AACGAAGCCA 
GGCATGCTCA ATGTGCACCT GCTGCCTCAT ACACACGATG ATGTAGGCTG GCTGAAGACA 
GTGGACCAAT ACTATTACGG CATCCTGAGT GATGTTCAGC ACGCATCTGT TCAGTACATC 
CTAGACTCAG TAGTCTCCTC TCTACTGGAG AAACCCACCC GTCGCTTCAT CTATGTGGAA 
ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAGTG CAACCCAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTGAT GAACGACGAA 
GCAGCCACCC ACTATGGCGC GATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAA 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ATATTGACCC TTTCGGCCAC 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTTGGGCGC 
ATTGACTATC AAGATAAACT TAATCGGAAG AAAAAGCTGA GGATGGAGGA GCTGTGGAGA 
GCTAGTGACA GCTTGGAGCC CCCCGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCCC CGAAATACCT GTGCTGGGAT GTGCTATGTA CAGACCCTCC CGTGGTGGAT 
AATCCCCGGA GCCCTGAGTT CAATGCAAAA ACGTTGGTCA ACTACTTCCT GAAGCTCGCT 
TCTTCCCAGA AAGGGTTTTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CATTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTGATCCG CCTGGTCAAC 
GCGCAGCAGG TAAACGGGAG CCTCGTCCAC GTTCTCTACT CCACCCCTAC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACCTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCCGATGGCC CCCACATGTT CTGGACTGGC TATTTTTCCA GCAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGTTACAA CTTCCTGCAG GTGTGTAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAAGCCA ACGTGGGACC CTATGGCTCG GGAGACAGCG CACCTCTTCA GGAGGCAATG 
GCGGTGCTCC AACACCACGA CGCTGTCAGT GGTACTGCAA GGCAGAATGT TGTAAATGAC 
TACGCGAGGC AGCTGGCAGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCATTACAA ACAGAACTTC TCATTCTGTC GTGAGCTCAA CATTAGCATC 
TGCCCAGTCA GCCAGACTTC GGAGCGTTTC CAGGTAACTC TTTACAACCC CCTGGGGCGA 
AAGGTGGATC AGATGGTTCG GCTGCCTGTC TATGAAGGCA ACTTCATTGT GAAGGACCCT 
CATGATAAGA ACATTTCCAG TAACGTGGTG ATGGTTCCTA GCTATTACAG TGAGACTTAC 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAC CTACTCTGTG 
GCCAAAATGA GTGACCTTAA CCACCAAGCC CACAACCTGC TGTCCAGGCC CAGGAAACAC 
AAGTCCCATC ATGTCTTAGT CATTGAAAAT AAGTACATGC GGGCCACATT TGACTCTGGC 
ACGGGGTTAT TGATGAAGAT TGAAAACTTG GAACAGAACC TCTCGCTCCC TGTGAGCCAA 
GGCTTCTTTT GGTATAATGC CAGTGTTGGT GATGAGGAGA GTTCTCAGGC CTCTGGGGCC 
TACATCTTCA GACCCAATGT CGGTAAACCG ATACCTGTGA GCCGCTGGGC TCAGATCAGT 
TTGGTGAAGA CAGCCTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTACAAAGG ACAGCGCCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAGAG ATGACTGGGG GAAGGAAGTC ATCAGCCGCT TTGACACCCC AATGAAAACC 
AAGGGACAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGATGATTAT 
CGACCCACCT GGACATTGAA TCAGACTGAG CCGGTGGCTG GAAATTACTA TCCTGTCAAC 
ACTCGAATTT ATATCACGGA TGGGCAAATG CAGCTGACCG TGTTGACCGA CCGCTCCCAG 
GGGGGCAGCA GTCTGCAGGA TGGCTCGTTG GAGCTCATGG TGCACCGTCG GCTGTTGGTA 
GATGATGATC GTGGAGTGTC GGAACCCTTG TTGGAGACAG ATACCGGGGA TAAGGTTCGA 
GGACGCCACC TTGTGCTTTT GAGTTCTGTC AGTGATGCCG CTGCAAGACA CCGACTGCTG 
GCGGAACAAG AAGTCCTGGC CCCTCAGGTG GTGCTGTCTC TGGGTGGTAG CAGTCCCTAT 
CACTCCCGGG CGACACCAAA AACGCAGTTT TCCGGACTTC GCCAGGAGCT ACCTCCTCAG 
GTGCATTTGC TCACACTGGC CCGCTGGGGG CCAAAGATGC TGCTGCTGCG CTTGGAGCAC 
CAGTTCGCTC TGAAAGAAGA TTCGGATCGC AACCTGAGCT CCCCTGTAAC CTTGAACGTT 
CAGAACCTGT TCCAGACCTT CACTATCAAC TACCTGCAGG AGACCACACT GGCAGCCAAC 
CAGCCCCTGT CCAGGGCTTC CAGGCTCAAG TGGATGACAA ATACTGGTCC CACCTCCTAC 
CCTGAGCCTT CCAAGTTGGA CCCTACCTCA GTCACACTGA AGCCTATGGA AATACGTACC 
TTCCTGGCCT CTGTACAATG GCAAGAACAC AGGCCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_006530747.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530810.1 Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X1, mRNA.

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CloneID	OMu41272
Clone ID Related Accession (Same CDS sequence)	XM_006530747.3 , XM_006530747.2
Accession Version	XM_006530747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG GACCGCTCAC CTCTGGTGTC CGTGCTGGTG GCGGGAACAC CGGGTGGCTC 
TGGATGAGCT CCTGCAACCT AGGTTCGCCA GTCCTGCCTA TTTCCTTTCT TTTTTGGCTG 
TTGCTGGCCG CGCCTGGCGC TCGGGCCGCG GGTTACAAGA CATGTCCCCC AACGAAGCCA 
GGCATGCTCA ATGTGCACCT GCTGCCTCAT ACACACGATG ATGTAGGCTG GCTGAAGACA 
GTGGACCAAT ACTATTACGG CATGGACCTT GACTTCCTGA ACTTCATGCC CACAGTCCTG 
AGTGATGTTC AGCACGCATC TGTTCAGTAC ATCCTAGACT CAGTAGTCTC CTCTCTACTG 
GAGAAACCCA CCCGTCGCTT CATCTATGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA GTGCAACCCA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT GATGAACGAC GAAGCAGCCA CCCACTATGG CGCGATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAAGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCATATTGA CCCTTTCGGC CACTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTTGGG CGCATTGACT ATCAAGATAA ACTTAATCGG 
AAGAAAAAGC TGAGGATGGA GGAGCTGTGG AGAGCTAGTG ACAGCTTGGA GCCCCCCGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CCCCGAAATA CCTGTGCTGG 
GATGTGCTAT GTACAGACCC TCCCGTGGTG GATAATCCCC GGAGCCCTGA GTTCAATGCA 
AAAACGTTGG TCAACTACTT CCTGAAGCTC GCTTCTTCCC AGAAAGGGTT TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCATTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTGAT CCGCCTGGTC AACGCGCAGC AGGTAAACGG GAGCCTCGTC 
CACGTTCTCT ACTCCACCCC TACGTGTTAC CTCTGGGAGC TGAACAAGGC CAATCTCACC 
TGGACAGTGA AGGAGGACGA CTTCTTTCCT TATGCCGATG GCCCCCACAT GTTCTGGACT 
GGCTATTTTT CCAGCAGGCC AGCCCTCAAG CGCTATGAGC GCCTCAGTTA CAACTTCCTG 
CAGGTGTGTA ACCAGCTGGA AGCACTGGTG GGTCCCGAAG CCAACGTGGG ACCCTATGGC 
TCGGGAGACA GCGCACCTCT TCAGGAGGCA ATGGCGGTGC TCCAACACCA CGACGCTGTC 
AGTGGTACTG CAAGGCAGAA TGTTGTAAAT GACTACGCGA GGCAGCTGGC AGCAGGCTGG 
GGACCCTGTG AGGTTCTCGT GAGCAACGCT CTGGCGCGAC TCAGCCATTA CAAACAGAAC 
TTCTCATTCT GTCGTGAGCT CAACATTAGC ATCTGCCCAG TCAGCCAGAC TTCGGAGCGT 
TTCCAGGTAA CTCTTTACAA CCCCCTGGGG CGAAAGGTGG ATCAGATGGT TCGGCTGCCT 
GTCTATGAAG GCAACTTCAT TGTGAAGGAC CCTCATGATA AGAACATTTC CAGTAACGTG 
GTGATGGTTC CTAGCTATTA CAGTGAGACT TACCAATGGG AGCTGCTGTT CCCAGCCTCA 
GTGCCTGCCC TGGGCTTCAG CACCTACTCT GTGGCCAAAA TGAGTGACCT TAACCACCAA 
GCCCACAACC TGCTGTCCAG GCCCAGGAAA CACAAGTCCC ATCATGTCTT AGTCATTGAA 
AATAAGTACA TGCGGGCCAC ATTTGACTCT GGCACGGGGT TATTGATGAA GATTGAAAAC 
TTGGAACAGA ACCTCTCGCT CCCTGTGAGC CAAGGCTTCT TTTGGTATAA TGCCAGTGTT 
GGTGATGAGG AGAGTTCTCA GGCCTCTGGG GCCTACATCT TCAGACCCAA TGTCGGTAAA 
CCGATACCTG TGAGCCGCTG GGCTCAGATC AGTTTGGTGA AGACAGCCTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTACAA AGGACAGCGC 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA GAGATGACTG GGGGAAGGAA 
GTCATCAGCC GCTTTGACAC CCCAATGAAA ACCAAGGGAC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGATGAT TATCGACCCA CCTGGACATT GAATCAGACT 
GAGCCGGTGG CTGGAAATTA CTATCCTGTC AACACTCGAA TTTATATCAC GGATGGGCAA 
ATGCAGCTGA CCGTGTTGAC CGACCGCTCC CAGGGGGGCA GCAGTCTGCA GGATGGCTCG 
TTGGAGCTCA TGGTGCACCG TCGGCTGTTG GTAGATGATG ATCGTGGAGT GTCGGAACCC 
TTGTTGGAGA CAGATACCGG GGATAAGGTT CGAGGACGCC ACCTTGTGCT TTTGAGTTCT 
GTCAGTGATG CCGCTGCAAG ACACCGACTG CTGGCGGAAC AAGAAGTCCT GGCCCCTCAG 
GTGGTGCTGT CTCTGGGTGG TAGCAGTCCC TATCACTCCC GGGCGACACC AAAAACGCAG 
TTTTCCGGAC TTCGCCAGGA GCTACCTCCT CAGGTGCATT TGCTCACACT GGCCCGCTGG 
GGGCCAAAGA TGCTGCTGCT GCGCTTGGAG CACCAGTTCG CTCTGAAAGA AGATTCGGAT 
CGCAACCTGA GCTCCCCTGT AACCTTGAAC GTTCAGAACC TGTTCCAGAC CTTCACTATC 
AACTACCTGC AGGAGACCAC ACTGGCAGCC AACCAGCCCC TGTCCAGGGC TTCCAGGCTC 
AAGTGGATGA CAAATACTGG TCCCACCTCC TACCCTGAGC CTTCCAAGTT GGACCCTACC 
TCAGTCACAC TGAAGCCTAT GGAAATACGT ACCTTCCTGG CCTCTGTACA ATGGCAAGAA 
CACAGGCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530810.1
CDS	600..3674
Translation	MGTGPLTSGVRAGGGNTGWLWMSSCNLGSPVLPISFLFWLLLAAPGARAAGYKTCPPTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGMDLDFLNFMPTVLSDVQHASVQYILDSVVSSLLEKPTRRFIYVEMAFFSRWWKQQTSATQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVDNPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQVNGSLVHVLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNFSFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETYQWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENLEQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQVIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTEPVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSYPEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA

Target ORF information:

RefSeq Version	XM_006530747.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha B1 (Man2b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530747.2

ORF Insert Sequence:

ATGGGCACCG GACCGCTCAC CTCTGGTGTC CGTGCTGGTG GCGGGAACAC CGGGTGGCTC 
TGGATGAGCT CCTGCAACCT AGGTTCGCCA GTCCTGCCTA TTTCCTTTCT TTTTTGGCTG 
TTGCTGGCCG CGCCTGGCGC TCGGGCCGCG GGTTACAAGA CATGTCCCCC AACGAAGCCA 
GGCATGCTCA ATGTGCACCT GCTGCCTCAT ACACACGATG ATGTAGGCTG GCTGAAGACA 
GTGGACCAAT ACTATTACGG CATGGACCTT GACTTCCTGA ACTTCATGCC CACAGTCCTG 
AGTGATGTTC AGCACGCATC TGTTCAGTAC ATCCTAGACT CAGTAGTCTC CTCTCTACTG 
GAGAAACCCA CCCGTCGCTT CATCTATGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA GTGCAACCCA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT GATGAACGAC GAAGCAGCCA CCCACTATGG CGCGATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAAGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCATATTGA CCCTTTCGGC CACTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTTGGG CGCATTGACT ATCAAGATAA ACTTAATCGG 
AAGAAAAAGC TGAGGATGGA GGAGCTGTGG AGAGCTAGTG ACAGCTTGGA GCCCCCCGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CCCCGAAATA CCTGTGCTGG 
GATGTGCTAT GTACAGACCC TCCCGTGGTG GATAATCCCC GGAGCCCTGA GTTCAATGCA 
AAAACGTTGG TCAACTACTT CCTGAAGCTC GCTTCTTCCC AGAAAGGGTT TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCATTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTGAT CCGCCTGGTC AACGCGCAGC AGGTAAACGG GAGCCTCGTC 
CACGTTCTCT ACTCCACCCC TACGTGTTAC CTCTGGGAGC TGAACAAGGC CAATCTCACC 
TGGACAGTGA AGGAGGACGA CTTCTTTCCT TATGCCGATG GCCCCCACAT GTTCTGGACT 
GGCTATTTTT CCAGCAGGCC AGCCCTCAAG CGCTATGAGC GCCTCAGTTA CAACTTCCTG 
CAGGTGTGTA ACCAGCTGGA AGCACTGGTG GGTCCCGAAG CCAACGTGGG ACCCTATGGC 
TCGGGAGACA GCGCACCTCT TCAGGAGGCA ATGGCGGTGC TCCAACACCA CGACGCTGTC 
AGTGGTACTG CAAGGCAGAA TGTTGTAAAT GACTACGCGA GGCAGCTGGC AGCAGGCTGG 
GGACCCTGTG AGGTTCTCGT GAGCAACGCT CTGGCGCGAC TCAGCCATTA CAAACAGAAC 
TTCTCATTCT GTCGTGAGCT CAACATTAGC ATCTGCCCAG TCAGCCAGAC TTCGGAGCGT 
TTCCAGGTAA CTCTTTACAA CCCCCTGGGG CGAAAGGTGG ATCAGATGGT TCGGCTGCCT 
GTCTATGAAG GCAACTTCAT TGTGAAGGAC CCTCATGATA AGAACATTTC CAGTAACGTG 
GTGATGGTTC CTAGCTATTA CAGTGAGACT TACCAATGGG AGCTGCTGTT CCCAGCCTCA 
GTGCCTGCCC TGGGCTTCAG CACCTACTCT GTGGCCAAAA TGAGTGACCT TAACCACCAA 
GCCCACAACC TGCTGTCCAG GCCCAGGAAA CACAAGTCCC ATCATGTCTT AGTCATTGAA 
AATAAGTACA TGCGGGCCAC ATTTGACTCT GGCACGGGGT TATTGATGAA GATTGAAAAC 
TTGGAACAGA ACCTCTCGCT CCCTGTGAGC CAAGGCTTCT TTTGGTATAA TGCCAGTGTT 
GGTGATGAGG AGAGTTCTCA GGCCTCTGGG GCCTACATCT TCAGACCCAA TGTCGGTAAA 
CCGATACCTG TGAGCCGCTG GGCTCAGATC AGTTTGGTGA AGACAGCCTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTACAA AGGACAGCGC 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA GAGATGACTG GGGGAAGGAA 
GTCATCAGCC GCTTTGACAC CCCAATGAAA ACCAAGGGAC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGATGAT TATCGACCCA CCTGGACATT GAATCAGACT 
GAGCCGGTGG CTGGAAATTA CTATCCTGTC AACACTCGAA TTTATATCAC GGATGGGCAA 
ATGCAGCTGA CCGTGTTGAC CGACCGCTCC CAGGGGGGCA GCAGTCTGCA GGATGGCTCG 
TTGGAGCTCA TGGTGCACCG TCGGCTGTTG GTAGATGATG ATCGTGGAGT GTCGGAACCC 
TTGTTGGAGA CAGATACCGG GGATAAGGTT CGAGGACGCC ACCTTGTGCT TTTGAGTTCT 
GTCAGTGATG CCGCTGCAAG ACACCGACTG CTGGCGGAAC AAGAAGTCCT GGCCCCTCAG 
GTGGTGCTGT CTCTGGGTGG TAGCAGTCCC TATCACTCCC GGGCGACACC AAAAACGCAG 
TTTTCCGGAC TTCGCCAGGA GCTACCTCCT CAGGTGCATT TGCTCACACT GGCCCGCTGG 
GGGCCAAAGA TGCTGCTGCT GCGCTTGGAG CACCAGTTCG CTCTGAAAGA AGATTCGGAT 
CGCAACCTGA GCTCCCCTGT AACCTTGAAC GTTCAGAACC TGTTCCAGAC CTTCACTATC 
AACTACCTGC AGGAGACCAC ACTGGCAGCC AACCAGCCCC TGTCCAGGGC TTCCAGGCTC 
AAGTGGATGA CAAATACTGG TCCCACCTCC TACCCTGAGC CTTCCAAGTT GGACCCTACC 
TCAGTCACAC TGAAGCCTAT GGAAATACGT ACCTTCCTGG CCTCTGTACA ATGGCAAGAA 
CACAGGCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DLK1 is a novel regulator of bone mass that mediates estrogen deficiency-induced bone loss in mice.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research26(7)1457-71(2011 Jul)
Abdallah BM,Ditzel N,Mahmood A,Isa A,Traustadottir GA,Schilling AF,Ruiz-Hidalgo MJ,Laborda J,Amling M,Kassem M

Impaired lysosomal trimming of N-linked oligosaccharides leads to hyperglycosylation of native lysosomal proteins in mice with alpha-mannosidosis.
Molecular and cellular biology30(1)273-83(2010 Jan)
Damme M,Morelle W,Schmidt B,Andersson C,Fogh J,Michalski JC,L?bke T

Liver sinusoidal endothelial cells depend on mannose receptor-mediated recruitment of lysosomal enzymes for normal degradation capacity.
Hepatology (Baltimore, Md.)48(6)2007-15(2008 Dec)
Elvevold K,Simon-Santamaria J,Hasvold H,McCourt P,Smedsr?d B,S?rensen KK

Characterization of the murine Inpp4b gene and identification of a novel isoform.
Gene376(1)152-61(2006 Jul)
Ferron M,Vacher J

Neurocognitive and psychotiform behavioral alterations and enhanced hippocampal long-term potentiation in transgenic mice displaying neuropathological features of human alpha-mannosidosis.
The Journal of neuroscience : the official journal of the Society for Neuroscience25(28)6539-49(2005 Jul)
D'Hooge R,L?llmann-Rauch R,Beckers T,Balschun D,Schwake M,Reiss K,von Figura K,Saftig P

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Man2b1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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