Man1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man1a
Entrez Gene ID	17155
Full Name	mannosidase 1, alpha
Synonyms	Man1a1,PCR1
General protein information	Preferred Names mannosidase 1, alpha Names mannosyl-oligosaccharide 1,2-alpha-mannosidase IA alpha-1,2-mannosidase IA man(9)-alpha-mannosidase man9-mannosidase mannosidase alpha class 1A member 1 mannosyl-oligosaccharide alpha-1,2-mannosidase processing alpha-1,2-mannosidase IA
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512569.4	XP_006512632.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X2
XM_011243136.1	XP_011241438.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X3
NM_008548.4	NP_032574.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
XM_006512568.2	XP_006512631.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X1
XM_030244925.1	XP_030100785.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X4
XM_006512569.3	XP_006512632.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X2
XM_006512568.3	XP_006512631.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X1
NM_008548.5	NP_032574.1	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MAN1A1	XP_419762.4
Danio rerio (zebrafish)	man1a1	XP_694435.2
Arabidopsis thaliana (thale cress)	MNS1	NP_175570.1
Oryza sativa (rice)	Os04g0606400	NP_001053800.1
Canis lupus familiaris (dog)	MAN1A1	XP_533481.3
Xenopus tropicalis (tropical clawed frog)	man1a1	XP_002932159.1
Rattus norvegicus (Norway rat)	Man1a1	NP_001028828.1
Arabidopsis thaliana (thale cress)	MNS2	NP_566675.1
Homo sapiens (human)	MAN1A1	NP_005898.2
Pan troglodytes (chimpanzee)	MAN1A1	XP_518868.3
Bos taurus (cattle)	LOC530027	XP_005210790.1
Mus musculus (house mouse)	Man1a	NP_032574.1
Caenorhabditis elegans (roundworm)	D2030.1	NP_492116.1

Gene Ontology
Bibliography

Related articles in PubMed

Substrate recognition and catalysis by GH47 ?-mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway.
Xiang Y, Karaveg K, Moremen KW
Proceedings of the National Academy of Sciences of the United States of America113(49)E7890-E7899(2016 Dec)

Differential expression and function of ?-mannosidase I in stimulated naive and memory CD4+ T cells.
Gebuhr I, Keeren K, Vogt K, H?flich C, Appelt C, Schlieer U, Brandt C, Meisel C, Volk HD, Sawitzki B
Journal of immunotherapy (Hagerstown, Md. : 1997)34(5)428-37(2011 Jun)

Zbtb20 is essential for the specification of CA1 field identity in the developing hippocampus.
Xie Z, Ma X, Ji W, Zhou G, Lu Y, Xiang Z, Wang YX, Zhang L, Hu Y, Ding YQ, Zhang WJ
Proceedings of the National Academy of Sciences of the United States of America107(14)6510-5(2010 Apr)

Identification of gene markers for the prediction of allograft rejection or permanent acceptance.
Sawitzki B, Bushell A, Steger U, Jones N, Risch K, Siepert A, Lehmann M, Schmitt-Knosalla I, Vogt K, Gebuhr I, Wood K, Volk HD
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons7(5)1091-102(2007 May)

Respiratory distress and neonatal lethality in mice lacking Golgi alpha1,2-mannosidase IB involved in N-glycan maturation.
Tremblay LO, Nagy Kov?cs E, Daniels E, Wong NK, Sutton-Smith M, Morris HR, Dell A, Marcinkiewicz E, Seidah NG, McKerlie C, Herscovics A
The Journal of biological chemistry282(4)2558-66(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Complex N-glycans generated by enzymes such as a-mannosidase I inhibit the activation of naive T cells.
Title: Differential expression and function of ?-mannosidase I in stimulated naive and memory CD4+ T cells.

Downregulation of TOAG-1 and alpha-1,2-mannosidase expression occurred in graft infiltrating cells and expression of both genes was also downregulated after T-cell activation in vitro
Title: Identification of gene markers for the prediction of allograft rejection or permanent acceptance.

Data report the crystallization of murine Golgi mannosidase IA and the structural solution to 1.5-A resolution by molecular replacement
Title: Structure of mouse Golgi alpha-mannosidase IA reveals the molecular basis for substrate specificity among class 1 (family 47 glycosylhydrolase) alpha1,2-mannosidases.

The following Man1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02201	NM_008548.5 Latest version!	Mus musculus mannosidase 1, alpha (Man1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu68175	XM_011243136.1 Latest version!	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu85373	XM_030244925.1 Latest version!	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu02201	XM_006512569.4 Latest version!	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu43740	XM_006512568.3 Latest version!	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu02201	XM_006512569.3	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu43740	XM_006512568.2	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu02201	NM_008548.4	Mus musculus mannosidase 1, alpha (Man1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man1a ( NM_008548.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032574.1 Mus musculus mannosidase 1, alpha (Man1a), mRNA.

Price & Availability↑

CloneID	OMu02201
Clone ID Related Accession (Same CDS sequence)	XM_006512569.4 , NM_008548.4 , XM_006512569.3 , NM_008548.5
Accession Version	NM_008548.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-22
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC158642.8, AC164624.3 and AC153816.4. On Apr 27, 2007 this sequence version replaced NM_008548.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031038.1, AK154820.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032574.1
CDS	518..2485
Translation	MPVGGLLPLFSSPGGGGLGSGLGGGLGGGRKGSGPAAFRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSNPALQPPAEHKPGLGARAEDAAEGRVRHREEGAPGDPGAGLEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKEKVAQDQLRDKDLFRGLPKVDFLPPVGVENREPADATIREKRAKIKEMMTHAWNNYKRYAWGLNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNAEVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	NM_008548.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008548.4

ORF Insert Sequence:

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man1a ( NM_008548.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032574.1 Mus musculus mannosidase 1, alpha (Man1a), mRNA.

Price & Availability↑

CloneID	OMu02201
Clone ID Related Accession (Same CDS sequence)	XM_006512569.4 , NM_008548.4 , XM_006512569.3 , NM_008548.5
Accession Version	NM_008548.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158642.8, AC164624.3 and AC153816.4. On Feb 7, 2019 this sequence version replaced NM_008548.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7974084.6618.1, AK154820.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032574.1
CDS	459..2426
Translation	MPVGGLLPLFSSPGGGGLGSGLGGGLGGGRKGSGPAAFRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSNPALQPPAEHKPGLGARAEDAAEGRVRHREEGAPGDPGAGLEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKEKVAQDQLRDKDLFRGLPKVDFLPPVGVENREPADATIREKRAKIKEMMTHAWNNYKRYAWGLNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNAEVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	NM_008548.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008548.5

ORF Insert Sequence:

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man1a ( XM_006512569.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512632.1 Mus musculus mannosidase 1, alpha (Man1a), transcript variant X2, mRNA.

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CloneID	OMu02201
Clone ID Related Accession (Same CDS sequence)	XM_006512569.4 , NM_008548.4 , XM_006512569.3 , NM_008548.5
Accession Version	XM_006512569.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512632.1
CDS	510..2477
Translation	MPVGGLLPLFSSPGGGGLGSGLGGGLGGGRKGSGPAAFRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSNPALQPPAEHKPGLGARAEDAAEGRVRHREEGAPGDPGAGLEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKEKVAQDQLRDKDLFRGLPKVDFLPPVGVENREPADATIREKRAKIKEMMTHAWNNYKRYAWGLNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNAEVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	XM_006512569.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512569.3

ORF Insert Sequence:

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man1a ( XM_011243136.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241438.1 Mus musculus mannosidase 1, alpha (Man1a), transcript variant X3, mRNA.

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CloneID	OMu68175
Clone ID Related Accession (Same CDS sequence)	XM_011243136.1
Accession Version	XM_011243136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGA CGGATGACTG GGCTCTGTCG CCTGAGTTGC TTTTTCAGTG GGACGAGATG 
ATGACCCATG CTTGGAATAA TTATAAACGC TATGCGTGGG GCTTGAACGA ACTGAAACCT 
ATATCAAAAG AAGGCCATTC AAGCAGTTTG TTTGGCAACA TCAAAGGAGC TACAATAGTA 
GATGCCCTGG ATACCCTTTT CATTATGGGC ATGAAGACTG AATTTCAAGA AGCTAAATCG 
TGGATTAAAA AATATTTAGA TTTTAATGTG AATGCTGAAG TTTCTGTTTT TGAAGTCAAC 
ATACGCTTCG TCGGTGGACT GCTGTCAGCC TACTATTTGT CCGGAGAGGA GATATTTCGA 
AAGAAAGCAG TGGAACTTGG GGTAAAATTG CTACCTGCAT TTCATACTCC CTCTGGAATA 
CCTTGGGCAT TGCTGAATAT GAAAAGTGGG ATCGGGCGGA ACTGGCCCTG GGCCTCTGGA 
GGCAGCAGTA TCCTGGCCGA ATTTGGAACT CTGCATTTAG AGTTTATGCA CTTGTCCCAC 
TTATCAGGAG ACCCAGTCTT TGCCGAAAAG GTTATGAAAA TTCGAACAGT GTTGAACAAA 
CTGGACAAAC CAGAAGGCCT TTATCCTAAC TATCTGAACC CCAGTAGTGG ACAGTGGGGT 
CAACATCATG TGTCGGTTGG AGGACTTGGA GACAGCTTTT ATGAATATTT GCTTAAGGCG 
TGGTTAATGT CTGACAAGAC AGATCTCGAA GCCAAGAAGA TGTATTTTGA TGCTGTTCAG 
GCCATCGAGA CTCACTTGAT CCGCAAGTCA AGTGGGGGAC TAACGTACAT CGCAGAGTGG 
AAGGGGGGCC TCCTGGAACA CAAGATGGGC CACCTGACGT GCTTTGCAGG AGGCATGTTT 
GCACTTGGGG CAGATGGAGC TCCGGAAGCC CGGGCCCAAC ACTACCTTGA ACTCGGAGCT 
GAAATTGCCC GCACTTGTCA TGAATCTTAT AATCGTACAT ATGTGAAGTT GGGACCGGAA 
GCGTTTCGAT TTGATGGCGG TGTGGAAGCT ATTGCCACGA GGCAAAATGA AAAGTATTAC 
ATCTTACGGC CCGAGGTCAT CGAGACATAC ATGTACATGT GGCGACTGAC TCACGACCCC 
AAGTACAGGA CCTGGGCCTG GGAAGCCGTG GAGGCTCTAG AAAGTCACTG CAGAGTGAAC 
GGAGGCTACT CAGGCTTACG GGATGTTTAC ATTGCCCGTG AGAGTTATGA CGATGTCCAG 
CAAAGTTTCT TCCTGGCAGA GACACTGAAG TATTTGTACT TGATATTTTC CGATGATGAC 
CTTCTTCCAC TAGAACACTG GATCTTCAAC ACCGAGGCTC ATCCTTTCCC TATACTCCGT 
GAACAGAAGA AGGAAATTGA TGGCAAAGAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241438.1
CDS	16..1431
Translation	MAGTDDWALSPELLFQWDEMMTHAWNNYKRYAWGLNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNAEVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	XM_011243136.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243136.1

ORF Insert Sequence:

ATGGCAGGGA CGGATGACTG GGCTCTGTCG CCTGAGTTGC TTTTTCAGTG GGACGAGATG 
ATGACCCATG CTTGGAATAA TTATAAACGC TATGCGTGGG GCTTGAACGA ACTGAAACCT 
ATATCAAAAG AAGGCCATTC AAGCAGTTTG TTTGGCAACA TCAAAGGAGC TACAATAGTA 
GATGCCCTGG ATACCCTTTT CATTATGGGC ATGAAGACTG AATTTCAAGA AGCTAAATCG 
TGGATTAAAA AATATTTAGA TTTTAATGTG AATGCTGAAG TTTCTGTTTT TGAAGTCAAC 
ATACGCTTCG TCGGTGGACT GCTGTCAGCC TACTATTTGT CCGGAGAGGA GATATTTCGA 
AAGAAAGCAG TGGAACTTGG GGTAAAATTG CTACCTGCAT TTCATACTCC CTCTGGAATA 
CCTTGGGCAT TGCTGAATAT GAAAAGTGGG ATCGGGCGGA ACTGGCCCTG GGCCTCTGGA 
GGCAGCAGTA TCCTGGCCGA ATTTGGAACT CTGCATTTAG AGTTTATGCA CTTGTCCCAC 
TTATCAGGAG ACCCAGTCTT TGCCGAAAAG GTTATGAAAA TTCGAACAGT GTTGAACAAA 
CTGGACAAAC CAGAAGGCCT TTATCCTAAC TATCTGAACC CCAGTAGTGG ACAGTGGGGT 
CAACATCATG TGTCGGTTGG AGGACTTGGA GACAGCTTTT ATGAATATTT GCTTAAGGCG 
TGGTTAATGT CTGACAAGAC AGATCTCGAA GCCAAGAAGA TGTATTTTGA TGCTGTTCAG 
GCCATCGAGA CTCACTTGAT CCGCAAGTCA AGTGGGGGAC TAACGTACAT CGCAGAGTGG 
AAGGGGGGCC TCCTGGAACA CAAGATGGGC CACCTGACGT GCTTTGCAGG AGGCATGTTT 
GCACTTGGGG CAGATGGAGC TCCGGAAGCC CGGGCCCAAC ACTACCTTGA ACTCGGAGCT 
GAAATTGCCC GCACTTGTCA TGAATCTTAT AATCGTACAT ATGTGAAGTT GGGACCGGAA 
GCGTTTCGAT TTGATGGCGG TGTGGAAGCT ATTGCCACGA GGCAAAATGA AAAGTATTAC 
ATCTTACGGC CCGAGGTCAT CGAGACATAC ATGTACATGT GGCGACTGAC TCACGACCCC 
AAGTACAGGA CCTGGGCCTG GGAAGCCGTG GAGGCTCTAG AAAGTCACTG CAGAGTGAAC 
GGAGGCTACT CAGGCTTACG GGATGTTTAC ATTGCCCGTG AGAGTTATGA CGATGTCCAG 
CAAAGTTTCT TCCTGGCAGA GACACTGAAG TATTTGTACT TGATATTTTC CGATGATGAC 
CTTCTTCCAC TAGAACACTG GATCTTCAAC ACCGAGGCTC ATCCTTTCCC TATACTCCGT 
GAACAGAAGA AGGAAATTGA TGGCAAAGAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man1a ( XM_030244925.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100785.1 Mus musculus mannosidase 1, alpha (Man1a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu85373
Clone ID Related Accession (Same CDS sequence)	XM_030244925.1
Accession Version	XM_030244925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TTCTGTTTTT GAAGTCAACA TACGCTTCGT CGGTGGACTG CTGTCAGCCT 
ACTATTTGTC CGGAGAGGAG TGGGATCGGG CGGAACTGGC CCTGGGCCTC TGGAGGCAGC 
AGTATCCTGG CCGAATTTGG AACTCTGCAT TTAGAGTTTA TGCACTTGTC CCACTTATCA 
GGAGACCCAG TCTTTGCCGA AAAGGTTATG AAAATTCGAA CAGTGTTGAA CAAACTGGAC 
AAACCAGAAG GCCTTTATCC TAACTATCTG AACCCCAGTA GTGGACAGTG GGGTCAACAT 
CATGTGTCGG TTGGAGGACT TGGAGACAGC TTTTATGAAT ATTTGCTTAA GGCGTGGTTA 
ATGTCTGACA AGACAGATCT CGAAGCCAAG AAGATGTATT TTGATGCTGT TCAGGCCATC 
GAGACTCACT TGATCCGCAA GTCAAGTGGG GGACTAACGT ACATCGCAGA GTGGAAGGGG 
GGCCTCCTGG AACACAAGAT GGGCCACCTG ACGTGCTTTG CAGGAGGCAT GTTTGCACTT 
GGGGCAGATG GAGCTCCGGA AGCCCGGGCC CAACACTACC TTGAACTCGG AGCTGAAATT 
GCCCGCACTT GTCATGAATC TTATAATCGT ACATATGTGA AGTTGGGACC GGAAGCGTTT 
CGATTTGATG GCGGTGTGGA AGCTATTGCC ACGAGGCAAA ATGAAAAGTA TTACATCTTA 
CGGCCCGAGG TCATCGAGAC ATACATGTAC ATGTGGCGAC TGACTCACGA CCCCAAGTAC 
AGGACCTGGG CCTGGGAAGC CGTGGAGGCT CTAGAAAGTC ACTGCAGAGT GAACGGAGGC 
TACTCAGGCT TACGGGATGT TTACATTGCC CGTGAGAGTT ATGACGATGT CCAGCAAAGT 
TTCTTCCTGG CAGAGACACT GAAGTATTTG TACTTGATAT TTTCCGATGA TGACCTTCTT 
CCACTAGAAC ACTGGATCTT CAACACCGAG GCTCATCCTT TCCCTATACT CCGTGAACAG 
AAGAAGGAAA TTGATGGCAA AGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100785.1
CDS	1353..2402
Translation	MLKFLFLKSTYASSVDCCQPTICPERSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	XM_030244925.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244925.1

ORF Insert Sequence:

ATGCTGAAGT TTCTGTTTTT GAAGTCAACA TACGCTTCGT CGGTGGACTG CTGTCAGCCT 
ACTATTTGTC CGGAGAGGAG TGGGATCGGG CGGAACTGGC CCTGGGCCTC TGGAGGCAGC 
AGTATCCTGG CCGAATTTGG AACTCTGCAT TTAGAGTTTA TGCACTTGTC CCACTTATCA 
GGAGACCCAG TCTTTGCCGA AAAGGTTATG AAAATTCGAA CAGTGTTGAA CAAACTGGAC 
AAACCAGAAG GCCTTTATCC TAACTATCTG AACCCCAGTA GTGGACAGTG GGGTCAACAT 
CATGTGTCGG TTGGAGGACT TGGAGACAGC TTTTATGAAT ATTTGCTTAA GGCGTGGTTA 
ATGTCTGACA AGACAGATCT CGAAGCCAAG AAGATGTATT TTGATGCTGT TCAGGCCATC 
GAGACTCACT TGATCCGCAA GTCAAGTGGG GGACTAACGT ACATCGCAGA GTGGAAGGGG 
GGCCTCCTGG AACACAAGAT GGGCCACCTG ACGTGCTTTG CAGGAGGCAT GTTTGCACTT 
GGGGCAGATG GAGCTCCGGA AGCCCGGGCC CAACACTACC TTGAACTCGG AGCTGAAATT 
GCCCGCACTT GTCATGAATC TTATAATCGT ACATATGTGA AGTTGGGACC GGAAGCGTTT 
CGATTTGATG GCGGTGTGGA AGCTATTGCC ACGAGGCAAA ATGAAAAGTA TTACATCTTA 
CGGCCCGAGG TCATCGAGAC ATACATGTAC ATGTGGCGAC TGACTCACGA CCCCAAGTAC 
AGGACCTGGG CCTGGGAAGC CGTGGAGGCT CTAGAAAGTC ACTGCAGAGT GAACGGAGGC 
TACTCAGGCT TACGGGATGT TTACATTGCC CGTGAGAGTT ATGACGATGT CCAGCAAAGT 
TTCTTCCTGG CAGAGACACT GAAGTATTTG TACTTGATAT TTTCCGATGA TGACCTTCTT 
CCACTAGAAC ACTGGATCTT CAACACCGAG GCTCATCCTT TCCCTATACT CCGTGAACAG 
AAGAAGGAAA TTGATGGCAA AGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man1a ( XM_006512568.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512631.1 Mus musculus mannosidase 1, alpha (Man1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43740
Clone ID Related Accession (Same CDS sequence)	XM_006512568.2 , XM_006512568.3
Accession Version	XM_006512568.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATT TGACCAACTG CGATGAGGGG CTGCAGCCAG CGGTTTGCCG CGCGTGCCTG 
TTTGCCAAAG GAGCCGCCCC GGCGAGGATC GCCGGAGGGG AGCCGCTTCA AGCGGGGAGA 
CCCGCAGCCC AGACACGCGC TCTGGGCGGC CTGGATGCGC AAGAGCCGGC CCCGCAGCCG 
CTGACAGCCG GCGGCAGCGA AGTTTGGCTG CTGAGCGGCT CGGCGCCGGG CCCCTGGACA 
GACTGTCAGC CGGCGGCTAC AACCTCCGGA GCGATGCCCG TGGGGGGCCT GTTGCCGCTC 
TTCAGTAGCC CTGGGGGCGG CGGCCTGGGC AGTGGCCTGG GCGGGGGGCT TGGCGGCGGG 
AGGAAGGGGT CTGGCCCCGC TGCCTTCCGC CTCACCGAGA AGTTCGTGCT GCTGCTGGTG 
TTCAGCGCCT TCATCACGCT CTGCTTCGGG GCAATCTTCT TCCTGCCTGA CTCCTCCAAG 
CTGCTCAGCG GGGTCCTGTT CCACTCCAAC CCTGCCTTGC AGCCGCCGGC GGAGCACAAG 
CCCGGGCTCG GGGCGCGTGC GGAGGATGCC GCCGAGGGGA GAGTCCGGCA CCGCGAGGAA 
GGCGCGCCTG GGGACCCTGG AGCTGGACTG GAAGACAACT TAGCCAGGAT CCGCGAAAAC 
CACGAGCGGG CTCTCAGGGA AGCCAAGGAG ACCCTGCAGA AGCTGCCGGA GGAGATCCAA 
AGAGACATTC TGCTGGAGAA GGAAAAGGTG GCCCAGGACC AGCTGCGTGA CAAGGATCTG 
TTTAGGGGCT TGCCCAAGGT GGACTTCCTG CCCCCCGTCG GGGTAGAGAA CCGGGAGCCC 
GCTGACGCCA CCATCCGTGA GAAGAGGGCA AAGATCAAAG AGATGATGAC CCATGCTTGG 
AATAATTATA AACGCTATGC GTGGGGCTTG AACGAACTGA AACCTATATC AAAAGAAGGC 
CATTCAAGCA GTTTGTTTGG CAACATCAAA GGAGCTACAA TAGTAGATGC CCTGGATACC 
CTTTTCATTA TGGGCATGAA GACTGAATTT CAAGAAGCTA AATCGTGGAT TAAAAAATAT 
TTAGATTTTA ATGTGAATGC TGAAGTTTCT GTTTTTGAAG TCAACATACG CTTCGTCGGT 
GGACTGCTGT CAGCCTACTA TTTGTCCGGA GAGGAGATAT TTCGAAAGAA AGCAGTGGAA 
CTTGGGGTAA AATTGCTACC TGCATTTCAT ACTCCCTCTG GAATACCTTG GGCATTGCTG 
AATATGAAAA GTGGGATCGG GCGGAACTGG CCCTGGGCCT CTGGAGGCAG CAGTATCCTG 
GCCGAATTTG GAACTCTGCA TTTAGAGTTT ATGCACTTGT CCCACTTATC AGGAGACCCA 
GTCTTTGCCG AAAAGGTTAT GAAAATTCGA ACAGTGTTGA ACAAACTGGA CAAACCAGAA 
GGCCTTTATC CTAACTATCT GAACCCCAGT AGTGGACAGT GGGGTCAACA TCATGTGTCG 
GTTGGAGGAC TTGGAGACAG CTTTTATGAA TATTTGCTTA AGGCGTGGTT AATGTCTGAC 
AAGACAGATC TCGAAGCCAA GAAGATGTAT TTTGATGCTG TTCAGGCCAT CGAGACTCAC 
TTGATCCGCA AGTCAAGTGG GGGACTAACG TACATCGCAG AGTGGAAGGG GGGCCTCCTG 
GAACACAAGA TGGGCCACCT GACGTGCTTT GCAGGAGGCA TGTTTGCACT TGGGGCAGAT 
GGAGCTCCGG AAGCCCGGGC CCAACACTAC CTTGAACTCG GAGCTGAAAT TGCCCGCACT 
TGTCATGAAT CTTATAATCG TACATATGTG AAGTTGGGAC CGGAAGCGTT TCGATTTGAT 
GGCGGTGTGG AAGCTATTGC CACGAGGCAA AATGAAAAGT ATTACATCTT ACGGCCCGAG 
GTCATCGAGA CATACATGTA CATGTGGCGA CTGACTCACG ACCCCAAGTA CAGGACCTGG 
GCCTGGGAAG CCGTGGAGGC TCTAGAAAGT CACTGCAGAG TGAACGGAGG CTACTCAGGC 
TTACGGGATG TTTACATTGC CCGTGAGAGT TATGACGATG TCCAGCAAAG TTTCTTCCTG 
GCAGAGACAC TGAAGTATTT GTACTTGATA TTTTCCGATG ATGACCTTCT TCCACTAGAA 
CACTGGATCT TCAACACCGA GGCTCATCCT TTCCCTATAC TCCGTGAACA GAAGAAGGAA 
ATTGATGGCA AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512631.1
CDS	52..2292
Translation	MENLTNCDEGLQPAVCRACLFAKGAAPARIAGGEPLQAGRPAAQTRALGGLDAQEPAPQPLTAGGSEVWLLSGSAPGPWTDCQPAATTSGAMPVGGLLPLFSSPGGGGLGSGLGGGLGGGRKGSGPAAFRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSNPALQPPAEHKPGLGARAEDAAEGRVRHREEGAPGDPGAGLEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKEKVAQDQLRDKDLFRGLPKVDFLPPVGVENREPADATIREKRAKIKEMMTHAWNNYKRYAWGLNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNAEVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	XM_006512568.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512568.2

ORF Insert Sequence:

ATGGAAAATT TGACCAACTG CGATGAGGGG CTGCAGCCAG CGGTTTGCCG CGCGTGCCTG 
TTTGCCAAAG GAGCCGCCCC GGCGAGGATC GCCGGAGGGG AGCCGCTTCA AGCGGGGAGA 
CCCGCAGCCC AGACACGCGC TCTGGGCGGC CTGGATGCGC AAGAGCCGGC CCCGCAGCCG 
CTGACAGCCG GCGGCAGCGA AGTTTGGCTG CTGAGCGGCT CGGCGCCGGG CCCCTGGACA 
GACTGTCAGC CGGCGGCTAC AACCTCCGGA GCGATGCCCG TGGGGGGCCT GTTGCCGCTC 
TTCAGTAGCC CTGGGGGCGG CGGCCTGGGC AGTGGCCTGG GCGGGGGGCT TGGCGGCGGG 
AGGAAGGGGT CTGGCCCCGC TGCCTTCCGC CTCACCGAGA AGTTCGTGCT GCTGCTGGTG 
TTCAGCGCCT TCATCACGCT CTGCTTCGGG GCAATCTTCT TCCTGCCTGA CTCCTCCAAG 
CTGCTCAGCG GGGTCCTGTT CCACTCCAAC CCTGCCTTGC AGCCGCCGGC GGAGCACAAG 
CCCGGGCTCG GGGCGCGTGC GGAGGATGCC GCCGAGGGGA GAGTCCGGCA CCGCGAGGAA 
GGCGCGCCTG GGGACCCTGG AGCTGGACTG GAAGACAACT TAGCCAGGAT CCGCGAAAAC 
CACGAGCGGG CTCTCAGGGA AGCCAAGGAG ACCCTGCAGA AGCTGCCGGA GGAGATCCAA 
AGAGACATTC TGCTGGAGAA GGAAAAGGTG GCCCAGGACC AGCTGCGTGA CAAGGATCTG 
TTTAGGGGCT TGCCCAAGGT GGACTTCCTG CCCCCCGTCG GGGTAGAGAA CCGGGAGCCC 
GCTGACGCCA CCATCCGTGA GAAGAGGGCA AAGATCAAAG AGATGATGAC CCATGCTTGG 
AATAATTATA AACGCTATGC GTGGGGCTTG AACGAACTGA AACCTATATC AAAAGAAGGC 
CATTCAAGCA GTTTGTTTGG CAACATCAAA GGAGCTACAA TAGTAGATGC CCTGGATACC 
CTTTTCATTA TGGGCATGAA GACTGAATTT CAAGAAGCTA AATCGTGGAT TAAAAAATAT 
TTAGATTTTA ATGTGAATGC TGAAGTTTCT GTTTTTGAAG TCAACATACG CTTCGTCGGT 
GGACTGCTGT CAGCCTACTA TTTGTCCGGA GAGGAGATAT TTCGAAAGAA AGCAGTGGAA 
CTTGGGGTAA AATTGCTACC TGCATTTCAT ACTCCCTCTG GAATACCTTG GGCATTGCTG 
AATATGAAAA GTGGGATCGG GCGGAACTGG CCCTGGGCCT CTGGAGGCAG CAGTATCCTG 
GCCGAATTTG GAACTCTGCA TTTAGAGTTT ATGCACTTGT CCCACTTATC AGGAGACCCA 
GTCTTTGCCG AAAAGGTTAT GAAAATTCGA ACAGTGTTGA ACAAACTGGA CAAACCAGAA 
GGCCTTTATC CTAACTATCT GAACCCCAGT AGTGGACAGT GGGGTCAACA TCATGTGTCG 
GTTGGAGGAC TTGGAGACAG CTTTTATGAA TATTTGCTTA AGGCGTGGTT AATGTCTGAC 
AAGACAGATC TCGAAGCCAA GAAGATGTAT TTTGATGCTG TTCAGGCCAT CGAGACTCAC 
TTGATCCGCA AGTCAAGTGG GGGACTAACG TACATCGCAG AGTGGAAGGG GGGCCTCCTG 
GAACACAAGA TGGGCCACCT GACGTGCTTT GCAGGAGGCA TGTTTGCACT TGGGGCAGAT 
GGAGCTCCGG AAGCCCGGGC CCAACACTAC CTTGAACTCG GAGCTGAAAT TGCCCGCACT 
TGTCATGAAT CTTATAATCG TACATATGTG AAGTTGGGAC CGGAAGCGTT TCGATTTGAT 
GGCGGTGTGG AAGCTATTGC CACGAGGCAA AATGAAAAGT ATTACATCTT ACGGCCCGAG 
GTCATCGAGA CATACATGTA CATGTGGCGA CTGACTCACG ACCCCAAGTA CAGGACCTGG 
GCCTGGGAAG CCGTGGAGGC TCTAGAAAGT CACTGCAGAG TGAACGGAGG CTACTCAGGC 
TTACGGGATG TTTACATTGC CCGTGAGAGT TATGACGATG TCCAGCAAAG TTTCTTCCTG 
GCAGAGACAC TGAAGTATTT GTACTTGATA TTTTCCGATG ATGACCTTCT TCCACTAGAA 
CACTGGATCT TCAACACCGA GGCTCATCCT TTCCCTATAC TCCGTGAACA GAAGAAGGAA 
ATTGATGGCA AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man1a ( XM_006512569.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512632.1 Mus musculus mannosidase 1, alpha (Man1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu02201
Clone ID Related Accession (Same CDS sequence)	XM_006512569.4 , NM_008548.4 , XM_006512569.3 , NM_008548.5
Accession Version	XM_006512569.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512569.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512632.1
CDS	555..2522
Translation	MPVGGLLPLFSSPGGGGLGSGLGGGLGGGRKGSGPAAFRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSNPALQPPAEHKPGLGARAEDAAEGRVRHREEGAPGDPGAGLEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKEKVAQDQLRDKDLFRGLPKVDFLPPVGVENREPADATIREKRAKIKEMMTHAWNNYKRYAWGLNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNAEVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	XM_006512569.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512569.4

ORF Insert Sequence:

ATGCCCGTGG GGGGCCTGTT GCCGCTCTTC AGTAGCCCTG GGGGCGGCGG CCTGGGCAGT 
GGCCTGGGCG GGGGGCTTGG CGGCGGGAGG AAGGGGTCTG GCCCCGCTGC CTTCCGCCTC 
ACCGAGAAGT TCGTGCTGCT GCTGGTGTTC AGCGCCTTCA TCACGCTCTG CTTCGGGGCA 
ATCTTCTTCC TGCCTGACTC CTCCAAGCTG CTCAGCGGGG TCCTGTTCCA CTCCAACCCT 
GCCTTGCAGC CGCCGGCGGA GCACAAGCCC GGGCTCGGGG CGCGTGCGGA GGATGCCGCC 
GAGGGGAGAG TCCGGCACCG CGAGGAAGGC GCGCCTGGGG ACCCTGGAGC TGGACTGGAA 
GACAACTTAG CCAGGATCCG CGAAAACCAC GAGCGGGCTC TCAGGGAAGC CAAGGAGACC 
CTGCAGAAGC TGCCGGAGGA GATCCAAAGA GACATTCTGC TGGAGAAGGA AAAGGTGGCC 
CAGGACCAGC TGCGTGACAA GGATCTGTTT AGGGGCTTGC CCAAGGTGGA CTTCCTGCCC 
CCCGTCGGGG TAGAGAACCG GGAGCCCGCT GACGCCACCA TCCGTGAGAA GAGGGCAAAG 
ATCAAAGAGA TGATGACCCA TGCTTGGAAT AATTATAAAC GCTATGCGTG GGGCTTGAAC 
GAACTGAAAC CTATATCAAA AGAAGGCCAT TCAAGCAGTT TGTTTGGCAA CATCAAAGGA 
GCTACAATAG TAGATGCCCT GGATACCCTT TTCATTATGG GCATGAAGAC TGAATTTCAA 
GAAGCTAAAT CGTGGATTAA AAAATATTTA GATTTTAATG TGAATGCTGA AGTTTCTGTT 
TTTGAAGTCA ACATACGCTT CGTCGGTGGA CTGCTGTCAG CCTACTATTT GTCCGGAGAG 
GAGATATTTC GAAAGAAAGC AGTGGAACTT GGGGTAAAAT TGCTACCTGC ATTTCATACT 
CCCTCTGGAA TACCTTGGGC ATTGCTGAAT ATGAAAAGTG GGATCGGGCG GAACTGGCCC 
TGGGCCTCTG GAGGCAGCAG TATCCTGGCC GAATTTGGAA CTCTGCATTT AGAGTTTATG 
CACTTGTCCC ACTTATCAGG AGACCCAGTC TTTGCCGAAA AGGTTATGAA AATTCGAACA 
GTGTTGAACA AACTGGACAA ACCAGAAGGC CTTTATCCTA ACTATCTGAA CCCCAGTAGT 
GGACAGTGGG GTCAACATCA TGTGTCGGTT GGAGGACTTG GAGACAGCTT TTATGAATAT 
TTGCTTAAGG CGTGGTTAAT GTCTGACAAG ACAGATCTCG AAGCCAAGAA GATGTATTTT 
GATGCTGTTC AGGCCATCGA GACTCACTTG ATCCGCAAGT CAAGTGGGGG ACTAACGTAC 
ATCGCAGAGT GGAAGGGGGG CCTCCTGGAA CACAAGATGG GCCACCTGAC GTGCTTTGCA 
GGAGGCATGT TTGCACTTGG GGCAGATGGA GCTCCGGAAG CCCGGGCCCA ACACTACCTT 
GAACTCGGAG CTGAAATTGC CCGCACTTGT CATGAATCTT ATAATCGTAC ATATGTGAAG 
TTGGGACCGG AAGCGTTTCG ATTTGATGGC GGTGTGGAAG CTATTGCCAC GAGGCAAAAT 
GAAAAGTATT ACATCTTACG GCCCGAGGTC ATCGAGACAT ACATGTACAT GTGGCGACTG 
ACTCACGACC CCAAGTACAG GACCTGGGCC TGGGAAGCCG TGGAGGCTCT AGAAAGTCAC 
TGCAGAGTGA ACGGAGGCTA CTCAGGCTTA CGGGATGTTT ACATTGCCCG TGAGAGTTAT 
GACGATGTCC AGCAAAGTTT CTTCCTGGCA GAGACACTGA AGTATTTGTA CTTGATATTT 
TCCGATGATG ACCTTCTTCC ACTAGAACAC TGGATCTTCA ACACCGAGGC TCATCCTTTC 
CCTATACTCC GTGAACAGAA GAAGGAAATT GATGGCAAAG AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man1a ( XM_006512568.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512631.1 Mus musculus mannosidase 1, alpha (Man1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43740
Clone ID Related Accession (Same CDS sequence)	XM_006512568.2 , XM_006512568.3
Accession Version	XM_006512568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mannosyl-oligosaccharide 1,2-alpha-mannosidase IA isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512568.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATT TGACCAACTG CGATGAGGGG CTGCAGCCAG CGGTTTGCCG CGCGTGCCTG 
TTTGCCAAAG GAGCCGCCCC GGCGAGGATC GCCGGAGGGG AGCCGCTTCA AGCGGGGAGA 
CCCGCAGCCC AGACACGCGC TCTGGGCGGC CTGGATGCGC AAGAGCCGGC CCCGCAGCCG 
CTGACAGCCG GCGGCAGCGA AGTTTGGCTG CTGAGCGGCT CGGCGCCGGG CCCCTGGACA 
GACTGTCAGC CGGCGGCTAC AACCTCCGGA GCGATGCCCG TGGGGGGCCT GTTGCCGCTC 
TTCAGTAGCC CTGGGGGCGG CGGCCTGGGC AGTGGCCTGG GCGGGGGGCT TGGCGGCGGG 
AGGAAGGGGT CTGGCCCCGC TGCCTTCCGC CTCACCGAGA AGTTCGTGCT GCTGCTGGTG 
TTCAGCGCCT TCATCACGCT CTGCTTCGGG GCAATCTTCT TCCTGCCTGA CTCCTCCAAG 
CTGCTCAGCG GGGTCCTGTT CCACTCCAAC CCTGCCTTGC AGCCGCCGGC GGAGCACAAG 
CCCGGGCTCG GGGCGCGTGC GGAGGATGCC GCCGAGGGGA GAGTCCGGCA CCGCGAGGAA 
GGCGCGCCTG GGGACCCTGG AGCTGGACTG GAAGACAACT TAGCCAGGAT CCGCGAAAAC 
CACGAGCGGG CTCTCAGGGA AGCCAAGGAG ACCCTGCAGA AGCTGCCGGA GGAGATCCAA 
AGAGACATTC TGCTGGAGAA GGAAAAGGTG GCCCAGGACC AGCTGCGTGA CAAGGATCTG 
TTTAGGGGCT TGCCCAAGGT GGACTTCCTG CCCCCCGTCG GGGTAGAGAA CCGGGAGCCC 
GCTGACGCCA CCATCCGTGA GAAGAGGGCA AAGATCAAAG AGATGATGAC CCATGCTTGG 
AATAATTATA AACGCTATGC GTGGGGCTTG AACGAACTGA AACCTATATC AAAAGAAGGC 
CATTCAAGCA GTTTGTTTGG CAACATCAAA GGAGCTACAA TAGTAGATGC CCTGGATACC 
CTTTTCATTA TGGGCATGAA GACTGAATTT CAAGAAGCTA AATCGTGGAT TAAAAAATAT 
TTAGATTTTA ATGTGAATGC TGAAGTTTCT GTTTTTGAAG TCAACATACG CTTCGTCGGT 
GGACTGCTGT CAGCCTACTA TTTGTCCGGA GAGGAGATAT TTCGAAAGAA AGCAGTGGAA 
CTTGGGGTAA AATTGCTACC TGCATTTCAT ACTCCCTCTG GAATACCTTG GGCATTGCTG 
AATATGAAAA GTGGGATCGG GCGGAACTGG CCCTGGGCCT CTGGAGGCAG CAGTATCCTG 
GCCGAATTTG GAACTCTGCA TTTAGAGTTT ATGCACTTGT CCCACTTATC AGGAGACCCA 
GTCTTTGCCG AAAAGGTTAT GAAAATTCGA ACAGTGTTGA ACAAACTGGA CAAACCAGAA 
GGCCTTTATC CTAACTATCT GAACCCCAGT AGTGGACAGT GGGGTCAACA TCATGTGTCG 
GTTGGAGGAC TTGGAGACAG CTTTTATGAA TATTTGCTTA AGGCGTGGTT AATGTCTGAC 
AAGACAGATC TCGAAGCCAA GAAGATGTAT TTTGATGCTG TTCAGGCCAT CGAGACTCAC 
TTGATCCGCA AGTCAAGTGG GGGACTAACG TACATCGCAG AGTGGAAGGG GGGCCTCCTG 
GAACACAAGA TGGGCCACCT GACGTGCTTT GCAGGAGGCA TGTTTGCACT TGGGGCAGAT 
GGAGCTCCGG AAGCCCGGGC CCAACACTAC CTTGAACTCG GAGCTGAAAT TGCCCGCACT 
TGTCATGAAT CTTATAATCG TACATATGTG AAGTTGGGAC CGGAAGCGTT TCGATTTGAT 
GGCGGTGTGG AAGCTATTGC CACGAGGCAA AATGAAAAGT ATTACATCTT ACGGCCCGAG 
GTCATCGAGA CATACATGTA CATGTGGCGA CTGACTCACG ACCCCAAGTA CAGGACCTGG 
GCCTGGGAAG CCGTGGAGGC TCTAGAAAGT CACTGCAGAG TGAACGGAGG CTACTCAGGC 
TTACGGGATG TTTACATTGC CCGTGAGAGT TATGACGATG TCCAGCAAAG TTTCTTCCTG 
GCAGAGACAC TGAAGTATTT GTACTTGATA TTTTCCGATG ATGACCTTCT TCCACTAGAA 
CACTGGATCT TCAACACCGA GGCTCATCCT TTCCCTATAC TCCGTGAACA GAAGAAGGAA 
ATTGATGGCA AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512631.1
CDS	128..2368
Translation	MENLTNCDEGLQPAVCRACLFAKGAAPARIAGGEPLQAGRPAAQTRALGGLDAQEPAPQPLTAGGSEVWLLSGSAPGPWTDCQPAATTSGAMPVGGLLPLFSSPGGGGLGSGLGGGLGGGRKGSGPAAFRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSNPALQPPAEHKPGLGARAEDAAEGRVRHREEGAPGDPGAGLEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKEKVAQDQLRDKDLFRGLPKVDFLPPVGVENREPADATIREKRAKIKEMMTHAWNNYKRYAWGLNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNAEVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIARTCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKYRTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNTEAHPFPILREQKKEIDGKEK

Target ORF information:

RefSeq Version	XM_006512568.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 1, alpha (Man1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512568.3

ORF Insert Sequence:

ATGGAAAATT TGACCAACTG CGATGAGGGG CTGCAGCCAG CGGTTTGCCG CGCGTGCCTG 
TTTGCCAAAG GAGCCGCCCC GGCGAGGATC GCCGGAGGGG AGCCGCTTCA AGCGGGGAGA 
CCCGCAGCCC AGACACGCGC TCTGGGCGGC CTGGATGCGC AAGAGCCGGC CCCGCAGCCG 
CTGACAGCCG GCGGCAGCGA AGTTTGGCTG CTGAGCGGCT CGGCGCCGGG CCCCTGGACA 
GACTGTCAGC CGGCGGCTAC AACCTCCGGA GCGATGCCCG TGGGGGGCCT GTTGCCGCTC 
TTCAGTAGCC CTGGGGGCGG CGGCCTGGGC AGTGGCCTGG GCGGGGGGCT TGGCGGCGGG 
AGGAAGGGGT CTGGCCCCGC TGCCTTCCGC CTCACCGAGA AGTTCGTGCT GCTGCTGGTG 
TTCAGCGCCT TCATCACGCT CTGCTTCGGG GCAATCTTCT TCCTGCCTGA CTCCTCCAAG 
CTGCTCAGCG GGGTCCTGTT CCACTCCAAC CCTGCCTTGC AGCCGCCGGC GGAGCACAAG 
CCCGGGCTCG GGGCGCGTGC GGAGGATGCC GCCGAGGGGA GAGTCCGGCA CCGCGAGGAA 
GGCGCGCCTG GGGACCCTGG AGCTGGACTG GAAGACAACT TAGCCAGGAT CCGCGAAAAC 
CACGAGCGGG CTCTCAGGGA AGCCAAGGAG ACCCTGCAGA AGCTGCCGGA GGAGATCCAA 
AGAGACATTC TGCTGGAGAA GGAAAAGGTG GCCCAGGACC AGCTGCGTGA CAAGGATCTG 
TTTAGGGGCT TGCCCAAGGT GGACTTCCTG CCCCCCGTCG GGGTAGAGAA CCGGGAGCCC 
GCTGACGCCA CCATCCGTGA GAAGAGGGCA AAGATCAAAG AGATGATGAC CCATGCTTGG 
AATAATTATA AACGCTATGC GTGGGGCTTG AACGAACTGA AACCTATATC AAAAGAAGGC 
CATTCAAGCA GTTTGTTTGG CAACATCAAA GGAGCTACAA TAGTAGATGC CCTGGATACC 
CTTTTCATTA TGGGCATGAA GACTGAATTT CAAGAAGCTA AATCGTGGAT TAAAAAATAT 
TTAGATTTTA ATGTGAATGC TGAAGTTTCT GTTTTTGAAG TCAACATACG CTTCGTCGGT 
GGACTGCTGT CAGCCTACTA TTTGTCCGGA GAGGAGATAT TTCGAAAGAA AGCAGTGGAA 
CTTGGGGTAA AATTGCTACC TGCATTTCAT ACTCCCTCTG GAATACCTTG GGCATTGCTG 
AATATGAAAA GTGGGATCGG GCGGAACTGG CCCTGGGCCT CTGGAGGCAG CAGTATCCTG 
GCCGAATTTG GAACTCTGCA TTTAGAGTTT ATGCACTTGT CCCACTTATC AGGAGACCCA 
GTCTTTGCCG AAAAGGTTAT GAAAATTCGA ACAGTGTTGA ACAAACTGGA CAAACCAGAA 
GGCCTTTATC CTAACTATCT GAACCCCAGT AGTGGACAGT GGGGTCAACA TCATGTGTCG 
GTTGGAGGAC TTGGAGACAG CTTTTATGAA TATTTGCTTA AGGCGTGGTT AATGTCTGAC 
AAGACAGATC TCGAAGCCAA GAAGATGTAT TTTGATGCTG TTCAGGCCAT CGAGACTCAC 
TTGATCCGCA AGTCAAGTGG GGGACTAACG TACATCGCAG AGTGGAAGGG GGGCCTCCTG 
GAACACAAGA TGGGCCACCT GACGTGCTTT GCAGGAGGCA TGTTTGCACT TGGGGCAGAT 
GGAGCTCCGG AAGCCCGGGC CCAACACTAC CTTGAACTCG GAGCTGAAAT TGCCCGCACT 
TGTCATGAAT CTTATAATCG TACATATGTG AAGTTGGGAC CGGAAGCGTT TCGATTTGAT 
GGCGGTGTGG AAGCTATTGC CACGAGGCAA AATGAAAAGT ATTACATCTT ACGGCCCGAG 
GTCATCGAGA CATACATGTA CATGTGGCGA CTGACTCACG ACCCCAAGTA CAGGACCTGG 
GCCTGGGAAG CCGTGGAGGC TCTAGAAAGT CACTGCAGAG TGAACGGAGG CTACTCAGGC 
TTACGGGATG TTTACATTGC CCGTGAGAGT TATGACGATG TCCAGCAAAG TTTCTTCCTG 
GCAGAGACAC TGAAGTATTT GTACTTGATA TTTTCCGATG ATGACCTTCT TCCACTAGAA 
CACTGGATCT TCAACACCGA GGCTCATCCT TTCCCTATAC TCCGTGAACA GAAGAAGGAA 
ATTGATGGCA AAGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Substrate recognition and catalysis by GH47 ?-mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway.
Proceedings of the National Academy of Sciences of the United States of America113(49)E7890-E7899(2016 Dec)
Xiang Y,Karaveg K,Moremen KW

Differential expression and function of ?-mannosidase I in stimulated naive and memory CD4+ T cells.
Journal of immunotherapy (Hagerstown, Md. : 1997)34(5)428-37(2011 Jun)
Gebuhr I,Keeren K,Vogt K,H?flich C,Appelt C,Schlieer U,Brandt C,Meisel C,Volk HD,Sawitzki B

Zbtb20 is essential for the specification of CA1 field identity in the developing hippocampus.
Proceedings of the National Academy of Sciences of the United States of America107(14)6510-5(2010 Apr)
Xie Z,Ma X,Ji W,Zhou G,Lu Y,Xiang Z,Wang YX,Zhang L,Hu Y,Ding YQ,Zhang WJ

Identification of gene markers for the prediction of allograft rejection or permanent acceptance.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons7(5)1091-102(2007 May)
Sawitzki B,Bushell A,Steger U,Jones N,Risch K,Siepert A,Lehmann M,Schmitt-Knosalla I,Vogt K,Gebuhr I,Wood K,Volk HD

Respiratory distress and neonatal lethality in mice lacking Golgi alpha1,2-mannosidase IB involved in N-glycan maturation.
The Journal of biological chemistry282(4)2558-66(2007 Jan)
Tremblay LO,Nagy Kov?cs E,Daniels E,Wong NK,Sutton-Smith M,Morris HR,Dell A,Marcinkiewicz E,Seidah NG,McKerlie C,Herscovics A

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Man1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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